FvH4_6g00941

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010575
Physical Location & Seq
Forward (+)
524595 .. 525148
554 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00941.t1

Sequence Viewer

Length: 429 bp
ATGGCACGCACAATTGGACACGAGAGAAGGTATGATAGCTGCTGGTCGCTCAATTGTAGTGTGGGGGCTATGGTTTGTATTTGTATCATGACTACCGTCGCCGTCGGTGCTTTTGTATGCATAGCTCTTATTTGTGTCGACCTACAACGTAATCCCACACCAGATGACCTAAAGATGGCTGTAACTGATGCATCCTTCACCGACGAGTTCAACATTACTAAGTGCAACAACTACAATCTCGACCTCAACATCACCCTTGGATATCGCAACAAGTATTTCCACATCTACTTCGGGAGGGCTCTAGTCGTCGCAGAATACCGCAACACGTCTGCATTGGCCAGCATGGCTGGCTTTCACCTACCGCCAAAGAACACGACGACTCTTCCGACGCCGTCGTTGAAACTGGAGCGGGAGCGAGGACATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

143

Amino Acids

15.95

Weight (kDa)

8.18

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 409
AccI GTMKAC 1 cut(s) 138
AciI CCGC 3 cut(s) 319, 362, 409
AcoI YGGCCR 1 cut(s) 336
AcyI GRCGYC 1 cut(s) 389
AfiI CCNNNNNNNGG 1 cut(s) 175
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 2 cut(s) 211, 400
AjiI CACGTC 1 cut(s) 327
AluBI AGCT 2 cut(s) 39, 125
AluI AGCT 2 cut(s) 39, 125
AoxI GGCC 1 cut(s) 336
ApeKI GCWGC 1 cut(s) 39
AsuHPI GGTGA 3 cut(s) 190, 244, 347
BalI TGGCCA 1 cut(s) 338
BanII GRGCYC 1 cut(s) 301
BauI CACGAG 1 cut(s) 20
BbvI GCAGC 1 cut(s) 26
BccI CCATC 1 cut(s) 169
BceAI ACGGC 2 cut(s) 86, 376
BfaI CTAG 1 cut(s) 302
BglI GCCNNNNNGGC 1 cut(s) 344
BisI GCNGC 1 cut(s) 40
BlsI GCNGC 1 cut(s) 41
BmgBI CACGTC 1 cut(s) 327
BmsI GCATC 2 cut(s) 178, 200
BoxI GACNNNNGTC 1 cut(s) 95
BpmI CTGGAG 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 256
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 1 cut(s) 191
BseLI CCNNNNNNNGG 1 cut(s) 175
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 1 cut(s) 26
BshFI GGCC 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 175
BsnI GGCC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 301
BspACI CCGC 3 cut(s) 319, 362, 409
BspANI GGCC 1 cut(s) 338
BspHI TCATGA 1 cut(s) 87
BsrBI CCGCTC 1 cut(s) 409
BsrI ACTGG 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 256
BssNI GRCGYC 1 cut(s) 389
BssSI CACGAG 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 256
Bst2BI CACGAG 1 cut(s) 20
Bst4CI ACNGT 1 cut(s) 97
Bst6I CTCTTC 1 cut(s) 387
BstACI GRCGYC 1 cut(s) 389
BstC8I GCNNGC 3 cut(s) 7, 340, 349
BstDEI CTNAG 1 cut(s) 219
BstF5I GGATG 1 cut(s) 191
BstMWI GCNNNNNNNGC 3 cut(s) 107, 344, 348
BstPAI GACNNNNGTC 1 cut(s) 95
BstV1I GCAGC 1 cut(s) 26
BsuRI GGCC 1 cut(s) 338
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 1 cut(s) 191
Cac8I GCNNGC 3 cut(s) 7, 340, 349
CciI TCATGA 1 cut(s) 87
CseI GACGC 1 cut(s) 397
CviAII CATG 2 cut(s) 88, 343
CviJI RGCY 8 cut(s) 39, 68, 125, 179, 299, 338, 347, 351
CviKI_1 RGCY 8 cut(s) 39, 68, 125, 179, 299, 338, 347, 351
DdeI CTNAG 1 cut(s) 219
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 301
Eco32I GATATC 1 cut(s) 263
EcoRV GATATC 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 256
EcoT22I ATGCAT 2 cut(s) 122, 193
EcoT38I GRGCYC 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 256
FaeI CATG 2 cut(s) 91, 346
FaiI YATR 6 cut(s) 33, 71, 89, 118, 122, 344
FatI CATG 2 cut(s) 87, 342
FauI CCCGC 1 cut(s) 402
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 1 cut(s) 40
FokI GGATG 1 cut(s) 178
FriOI GRGCYC 1 cut(s) 301
Fsp4HI GCNGC 1 cut(s) 40
FspBI CTAG 1 cut(s) 302
GluI GCNGC 1 cut(s) 40
GsuI CTGGAG 1 cut(s) 425
HaeIII GGCC 1 cut(s) 338
HgaI GACGC 1 cut(s) 397
Hin1I GRCGYC 1 cut(s) 389
Hin1II CATG 2 cut(s) 91, 346
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 1 cut(s) 379
HphI GGTGA 3 cut(s) 190, 244, 347
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 3 cut(s) 88, 239, 292
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 7 cut(s) 101, 107, 206, 311, 379, 391, 397
HpyAV CCTTC 2 cut(s) 21, 205
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 2 cut(s) 148, 326
HpyCH4V TGCA 4 cut(s) 120, 191, 225, 332
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 344, 348
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 2 cut(s) 148, 326
Hsp92I GRCGYC 1 cut(s) 389
Hsp92II CATG 2 cut(s) 91, 346
LmnI GCTCC 2 cut(s) 406, 412
LpnPI CCDG 5 cut(s) 28, 174, 333, 352, 389
Lsp1109I GCAGC 1 cut(s) 26
LweI GCATC 2 cut(s) 178, 200
MaeI CTAG 1 cut(s) 302
MaeII ACGT 2 cut(s) 148, 326
MaeIII GTNAC 1 cut(s) 181
MbiI CCGCTC 1 cut(s) 409
MboII GAAGA 1 cut(s) 374
MfeI CAATTG 2 cut(s) 12, 52
MhlI GDGCHC 1 cut(s) 301
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 2 cut(s) 12, 52
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 3 cut(s) 254, 288, 410
Mox20I TGGCCA 1 cut(s) 338
Mph1103I ATGCAT 2 cut(s) 122, 193
MscI TGGCCA 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 338
MunI CAATTG 2 cut(s) 12, 52
MwoI GCNNNNNNNGC 3 cut(s) 107, 344, 348
NlaIII CATG 2 cut(s) 91, 346
NsiI ATGCAT 2 cut(s) 122, 193
PagI TCATGA 1 cut(s) 87
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PflFI GACNNNGTC 1 cut(s) 391
PkrI GCNGC 1 cut(s) 41
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
PshAI GACNNNNGTC 1 cut(s) 95
PsyI GACNNNGTC 1 cut(s) 391
SalI GTCGAC 1 cut(s) 137
SatI GCNGC 1 cut(s) 40
SchI GAGTC 1 cut(s) 373
SduI GDGCHC 1 cut(s) 301
SetI ASST 9 cut(s) 32, 41, 127, 144, 151, 171, 246, 329, 360
SfaNI GCATC 2 cut(s) 178, 200
Sse9I AATT 2 cut(s) 12, 52
SsiI CCGC 3 cut(s) 319, 362, 409
SspMI CTAG 1 cut(s) 302
StyI CCWWGG 1 cut(s) 256
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 2 cut(s) 151, 329
TaqI TCGA 2 cut(s) 138, 240
TasI AATT 2 cut(s) 12, 52
TseI GCWGC 1 cut(s) 39
Tth111I GACNNNGTC 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 1 cut(s) 302
Zsp2I ATGCAT 2 cut(s) 122, 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.