RchiOBHm_Chr3g0448401

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0010575
Physical Location & Seq
Forward (+)
657296 .. 657904
609 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41584

Sequence Viewer

Length: 609 bp
ATGGGTCCATCAGGCATGTGCATCTGCAGTTCGATCTCATGCTTTGTAATAACCGTAACCGCGCTGATCATCGCTTGCACTACATACTCGATTGTAAGGGCTGAACAACTCCGCACCCCACCAGGTCCCTTAAAGATACACGTAACTGATGCCTCCCTCAACTACACCACCCTTGATCTGGCCCTCAATATCACCCTTGAAAACCCCAACAAGTATTTCCACATCTATTACGACGACTCTATAGTCATCGCTGGTTACAGCAACAAAACCTCTGCTATAGCGAGTGTCGCCGACTTTCACCAACTCGAAAATAATACGACTCTTCTGACGCCGTCGTTGAAAATGCATCGGGGTGATAGTACTGACGATGCAACTGGGTCGGGAAAAGGGAATTACTACGACATTGTCGTCAAGCTCTATCTTCCAAAGTGGTACAAGTCCATCTCCAAGAAGAGGGACAATTGGCAAAGCAGTATACATATGTACACATGTGAATTGAAGGTTCCTTTGAACAATGGATCAAAATCTGTTGATGCTTTCAATACTACCGAGTGCGCGCCAAGAACCGTTGATTTTGGTTTTCGCTATAGCTGGCGTAAGTTAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

202

Amino Acids

22.56

Weight (kDa)

8.46

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 242, 407
AccI GTMKAC 1 cut(s) 475
AccII CGCG 2 cut(s) 62, 557
AciI CCGC 2 cut(s) 60, 112
AclWI GGATC 1 cut(s) 526
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 3 cut(s) 361, 434, 485
AfiI CCNNNNNNNGG 2 cut(s) 178, 453
AflIII ACRYGT 2 cut(s) 139, 488
AgsI TTSAA 5 cut(s) 200, 340, 499, 511, 541
AjnI CCWGG 1 cut(s) 121
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 2 cut(s) 415, 591
AluI AGCT 2 cut(s) 415, 591
AlwI GGATC 1 cut(s) 526
AoxI GGCC 1 cut(s) 180
AspLEI GCGC 3 cut(s) 64, 557, 559
AspS9I GGNCC 3 cut(s) 5, 125, 181
AsuHPI GGTGA 3 cut(s) 184, 290, 365
AvaII GGWCC 2 cut(s) 5, 125
BccI CCATC 2 cut(s) 16, 449
BceAI ACGGC 1 cut(s) 316
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciT130I CCWGG 1 cut(s) 123
BclI TGATCA 1 cut(s) 66
BfmI CTRYAG 4 cut(s) 25, 240, 276, 586
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 2 cut(s) 5, 125
BmgT120I GGNCC 3 cut(s) 5, 125, 181
BmiI GGNNCC 3 cut(s) 6, 127, 504
BmrFI CCNGG 1 cut(s) 123
BmrI ACTGGG 1 cut(s) 384
BmsI GCATC 5 cut(s) 30, 139, 355, 358, 523
BmuI ACTGGG 1 cut(s) 384
BsaAI YACGTR 1 cut(s) 142
BsaBI GATNNNNATC 1 cut(s) 523
BsaHI GRCGYC 1 cut(s) 329
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 453
Bse1I ACTGG 1 cut(s) 379
Bse8I GATNNNNATC 1 cut(s) 523
BseBI CCWGG 1 cut(s) 123
BseJI GATNNNNATC 1 cut(s) 523
BseLI CCNNNNNNNGG 2 cut(s) 178, 453
BseNI ACTGG 1 cut(s) 379
BsePI GCGCGC 1 cut(s) 555
Bsh1236I CGCG 2 cut(s) 62, 557
BshFI GGCC 1 cut(s) 182
BslFI GGGAC 2 cut(s) 111, 470
BslI CCNNNNNNNGG 2 cut(s) 178, 453
BsmFI GGGAC 2 cut(s) 111, 470
BsnI GGCC 1 cut(s) 182
Bsp1407I TGTACA 1 cut(s) 483
Bsp143I GATC 4 cut(s) 33, 66, 175, 518
BspACI CCGC 2 cut(s) 60, 112
BspANI GGCC 1 cut(s) 182
BspFNI CGCG 2 cut(s) 62, 557
BspLI GGNNCC 3 cut(s) 6, 127, 504
BspMAI CTGCAG 1 cut(s) 29
BspPI GGATC 1 cut(s) 526
BsrGI TGTACA 1 cut(s) 483
BsrI ACTGG 1 cut(s) 379
BssHII GCGCGC 1 cut(s) 555
BssMI GATC 4 cut(s) 33, 66, 175, 518
BssNAI GTATAC 1 cut(s) 476
BssNI GRCGYC 1 cut(s) 329
Bst1107I GTATAC 1 cut(s) 476
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 2 cut(s) 55, 568
Bst6I CTCTTC 2 cut(s) 327, 446
BstACI GRCGYC 1 cut(s) 329
BstAUI TGTACA 1 cut(s) 483
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 3 cut(s) 76, 557, 593
BstFNI CGCG 2 cut(s) 62, 557
BstHHI GCGC 3 cut(s) 64, 557, 559
BstKTI GATC 4 cut(s) 36, 69, 178, 521
BstMBI GATC 4 cut(s) 33, 66, 175, 518
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 1 cut(s) 123
BstNSI RCATGY 2 cut(s) 19, 492
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 4 cut(s) 25, 240, 276, 586
BstUI CGCG 2 cut(s) 62, 557
BstZ17I GTATAC 1 cut(s) 476
BsuRI GGCC 1 cut(s) 182
BtgZI GCGATG 2 cut(s) 55, 232
Cac8I GCNNGC 3 cut(s) 76, 557, 593
CfoI GCGC 3 cut(s) 64, 557, 559
Cfr13I GGNCC 3 cut(s) 5, 125, 181
CseI GACGC 1 cut(s) 337
CsiI ACCWGGT 1 cut(s) 121
Csp6I GTAC 3 cut(s) 360, 433, 484
CviAII CATG 3 cut(s) 16, 39, 489
CviJI RGCY 4 cut(s) 101, 182, 415, 591
CviKI_1 RGCY 4 cut(s) 101, 182, 415, 591
CviQI GTAC 3 cut(s) 360, 433, 484
DpnI GATC 4 cut(s) 35, 68, 177, 520
DpnII GATC 4 cut(s) 33, 66, 175, 518
DrdI GACNNNNNNGTC 2 cut(s) 242, 407
DseDI GACNNNNNNGTC 2 cut(s) 242, 407
Eam1104I CTCTTC 2 cut(s) 327, 446
EarI CTCTTC 2 cut(s) 327, 446
Eco47I GGWCC 2 cut(s) 5, 125
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 348
FaeI CATG 3 cut(s) 19, 42, 492
FaqI GGGAC 2 cut(s) 111, 470
FatI CATG 3 cut(s) 15, 38, 488
FauNDI CATATG 1 cut(s) 480
FbaI TGATCA 1 cut(s) 66
FblI GTMKAC 1 cut(s) 475
GlaI GCGC 3 cut(s) 63, 556, 558
HaeIII GGCC 1 cut(s) 182
HgaI GACGC 1 cut(s) 337
HhaI GCGC 3 cut(s) 64, 557, 559
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 3 cut(s) 19, 42, 492
Hin6I GCGC 3 cut(s) 62, 555, 557
HinP1I GCGC 3 cut(s) 62, 555, 557
HinfI GANTC 2 cut(s) 236, 319
HphI GGTGA 3 cut(s) 184, 290, 365
Hpy166II GTNNAC 2 cut(s) 476, 486
Hpy188I TCNGA 1 cut(s) 327
Hpy188III TCNNGA 1 cut(s) 381
Hpy8I GTNNAC 2 cut(s) 476, 486
Hpy99I CGWCG 2 cut(s) 236, 337
HpyAV CCTTC 1 cut(s) 493
HpyCH4III ACNGT 2 cut(s) 55, 568
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 5 cut(s) 21, 27, 78, 346, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 3 cut(s) 19, 42, 492
HspAI GCGC 3 cut(s) 62, 555, 557
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 4 cut(s) 33, 66, 175, 518
LpnPI CCDG 6 cut(s) 108, 135, 164, 237, 360, 577
LweI GCATC 5 cut(s) 30, 139, 355, 358, 523
MabI ACCWGGT 1 cut(s) 121
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 55, 142, 254
MalI GATC 4 cut(s) 35, 68, 177, 520
MboI GATC 4 cut(s) 33, 66, 175, 518
MboII GAAGA 3 cut(s) 314, 413, 463
MfeI CAATTG 1 cut(s) 460
MluCI AATT 3 cut(s) 391, 460, 494
MlyI GAGTC 2 cut(s) 230, 313
MnlI CCTC 5 cut(s) 163, 167, 194, 280, 447
Mph1103I ATGCAT 1 cut(s) 348
MseI TTAA 1 cut(s) 131
MslI CAYNNNNRTG 1 cut(s) 351
MspR9I CCNGG 1 cut(s) 123
MunI CAATTG 1 cut(s) 460
MvaI CCWGG 1 cut(s) 123
MvnI CGCG 2 cut(s) 62, 557
MwoI GCNNNNNNNGC 1 cut(s) 287
NdeI CATATG 1 cut(s) 480
NdeII GATC 4 cut(s) 33, 66, 175, 518
NlaIII CATG 3 cut(s) 19, 42, 492
NlaIV GGNNCC 3 cut(s) 6, 127, 504
NsiI ATGCAT 1 cut(s) 348
NspI RCATGY 2 cut(s) 19, 492
PauI GCGCGC 1 cut(s) 555
PciI ACATGT 1 cut(s) 488
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PflFI GACNNNGTC 2 cut(s) 331, 404
PleI GAGTC 2 cut(s) 230, 313
PpsI GAGTC 2 cut(s) 230, 313
Ppu21I YACGTR 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 125
PscI ACATGT 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 3 cut(s) 6, 127, 504
PspPI GGNCC 3 cut(s) 5, 125, 181
PspPPI RGGWCCY 1 cut(s) 125
PstI CTGCAG 1 cut(s) 29
PsyI GACNNNGTC 2 cut(s) 331, 404
PteI GCGCGC 1 cut(s) 555
RsaI GTAC 3 cut(s) 361, 434, 485
RsaNI GTAC 3 cut(s) 360, 433, 484
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 1 cut(s) 131
Sau3AI GATC 4 cut(s) 33, 66, 175, 518
Sau96I GGNCC 3 cut(s) 5, 125, 181
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 2 cut(s) 230, 313
ScrFI CCNGG 1 cut(s) 123
SetI ASST 6 cut(s) 127, 144, 272, 417, 504, 593
SexAI ACCWGGT 1 cut(s) 121
SfaNI GCATC 5 cut(s) 30, 139, 355, 358, 523
SfcI CTRYAG 4 cut(s) 25, 240, 276, 586
SinI GGWCC 2 cut(s) 5, 125
SmiMI CAYNNNNRTG 1 cut(s) 351
Sse9I AATT 3 cut(s) 391, 460, 494
SsiI CCGC 2 cut(s) 60, 112
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 2 cut(s) 55, 568
TaiI ACGT 1 cut(s) 144
TaqI TCGA 3 cut(s) 32, 89, 306
TasI AATT 3 cut(s) 391, 460, 494
TatI WGTACW 2 cut(s) 359, 483
Tru1I TTAA 1 cut(s) 131
Tru9I TTAA 1 cut(s) 131
Tth111I GACNNNGTC 2 cut(s) 331, 404
VpaK11BI GGWCC 2 cut(s) 5, 125
XceI RCATGY 2 cut(s) 19, 492
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XmiI GTMKAC 1 cut(s) 475
ZrmI AGTACT 1 cut(s) 361
Zsp2I ATGCAT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.