RchiOBHm_Chr3g0448391

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0010575
Physical Location & Seq
Forward (+)
645310 .. 645978
669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41583

Sequence Viewer

Length: 555 bp
ATGGCACGCACGAGTGGATGCCAGAGAAGATCTACCACGGCTGCTCAAGATTCCGATCGGGAAACCGAGCACTGCCGCTGTTGGATTATCTTACTCGTGGTGTTCCTTTTCCTTTTACTGGTGTTTTATTTCGTCGAGAGCATAATCAGAAGCGGGCCACCAGATGACATAAAGATCACTGTAACTGATGCCTCCCTCACCGAGTTCAACATAACCAGCTCCAACAACTACAATCTCGACCTCAACATCACCCTTCGAAATCCCAACAAGGATTTTGGAATCTACTACCGGTACAAGGCTCTAGTCGTCGCAGACTACCGCAAGACGTCTGCTATGGCCAGCATTGCTAACTTTCACCAACCAGCAAAGAATACGACTCTTCTGACGCCGTCGTTTAAACTGCAGCGGGATAATATTACTGGCGATGCAGCTGCGTCAGGGAAAGGGAATTACTACGACATTGTCGTTACCCTCTATCTTCCAAAGAAGTACGTACAAGTCCGTCACCTGGTGGAAGAACGATTGGCTAAGCAGTATACAACTCTACGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

184

Amino Acids

21.05

Weight (kDa)

9.24

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 329
AccI GTMKAC 1 cut(s) 536
AciI CCGC 4 cut(s) 76, 153, 319, 406
AcoI YGGCCR 1 cut(s) 336
AcyI GRCGYC 2 cut(s) 326, 386
AdeI CACNNNGTG 1 cut(s) 511
AfaI GTAC 3 cut(s) 293, 491, 495
AfiI CCNNNNNNNGG 3 cut(s) 118, 295, 508
AgeI ACCGGT 1 cut(s) 288
AgsI TTSAA 1 cut(s) 208
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 2 cut(s) 219, 431
AluI AGCT 2 cut(s) 219, 431
Alw21I GWGCWC 1 cut(s) 72
AoxI GGCC 2 cut(s) 155, 336
ApeKI GCWGC 4 cut(s) 41, 403, 428, 431
AsiGI ACCGGT 1 cut(s) 288
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 4 cut(s) 190, 241, 347, 497
AsuII TTCGAA 1 cut(s) 256
BalI TGGCCA 1 cut(s) 338
BauI CACGAG 2 cut(s) 10, 95
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 4 cut(s) 28, 415, 418, 440
BceAI ACGGC 2 cut(s) 54, 373
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 1 cut(s) 509
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 1 cut(s) 401
BglII AGATCT 1 cut(s) 29
BisI GCNGC 5 cut(s) 42, 76, 404, 429, 432
BlpI GCTNAGC 1 cut(s) 528
BlsI GCNGC 5 cut(s) 43, 77, 405, 430, 433
Bme1390I CCNGG 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 155
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 3 cut(s) 8, 178, 415
Bpu1102I GCTNAGC 1 cut(s) 528
Bpu14I TTCGAA 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 493
BsaBI GATNNNNATC 1 cut(s) 54
BsaHI GRCGYC 2 cut(s) 326, 386
BsaJI CCNNGG 1 cut(s) 36
BsaWI WCCGGW 1 cut(s) 288
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 295, 508
Bse118I RCCGGY 1 cut(s) 288
Bse1I ACTGG 2 cut(s) 123, 424
Bse3DI GCAATG 1 cut(s) 342
Bse8I GATNNNNATC 1 cut(s) 54
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 1 cut(s) 23
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 3 cut(s) 118, 295, 508
BseMI GCAATG 1 cut(s) 342
BseNI ACTGG 2 cut(s) 123, 424
BseXI GCAGC 4 cut(s) 28, 415, 418, 440
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 2 cut(s) 157, 338
BshTI ACCGGT 1 cut(s) 288
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 289
BslI CCNNNNNNNGG 3 cut(s) 118, 295, 508
BsnI GGCC 2 cut(s) 157, 338
Bsp119I TTCGAA 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 3 cut(s) 29, 55, 174
Bsp1720I GCTNAGC 1 cut(s) 528
BspACI CCGC 4 cut(s) 76, 153, 319, 406
BspANI GGCC 2 cut(s) 157, 338
BspMAI CTGCAG 1 cut(s) 405
BspT104I TTCGAA 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 288
BsrI ACTGG 2 cut(s) 123, 424
BssAI RCCGGY 1 cut(s) 288
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 3 cut(s) 29, 55, 174
BssNAI GTATAC 1 cut(s) 537
BssNI GRCGYC 2 cut(s) 326, 386
BssSI CACGAG 2 cut(s) 10, 95
Bst1107I GTATAC 1 cut(s) 537
Bst2BI CACGAG 2 cut(s) 10, 95
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 384
BstACI GRCGYC 2 cut(s) 326, 386
BstBAI YACGTR 1 cut(s) 493
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 3 cut(s) 7, 155, 340
BstDEI CTNAG 1 cut(s) 528
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 1 cut(s) 23
BstKTI GATC 3 cut(s) 32, 58, 177
BstMBI GATC 3 cut(s) 29, 55, 174
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 401
BstSNI TACGTA 1 cut(s) 493
BstV1I GCAGC 4 cut(s) 28, 415, 418, 440
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
BstZ17I GTATAC 1 cut(s) 537
BsuRI GGCC 2 cut(s) 157, 338
BtgI CCRYGG 1 cut(s) 36
BtgZI GCGATG 1 cut(s) 438
BtsCI GGATG 1 cut(s) 23
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 70, 177
Cac8I GCNNGC 3 cut(s) 7, 155, 340
Cfr10I RCCGGY 1 cut(s) 288
Cfr13I GGNCC 1 cut(s) 155
CseI GACGC 2 cut(s) 394, 423
CsiI ACCWGGT 1 cut(s) 507
Csp6I GTAC 3 cut(s) 292, 490, 494
CspAI ACCGGT 1 cut(s) 288
CviAII CATG 1 cut(s) 550
CviJI RGCY 7 cut(s) 41, 157, 219, 299, 338, 431, 527
CviKI_1 RGCY 7 cut(s) 41, 157, 219, 299, 338, 431, 527
CviQI GTAC 3 cut(s) 292, 490, 494
DdeI CTNAG 1 cut(s) 528
DpnI GATC 3 cut(s) 31, 57, 176
DpnII GATC 3 cut(s) 29, 55, 174
DraI TTTAAA 1 cut(s) 397
DraIII CACNNNGTG 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
Eco105I TACGTA 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 507
FaeI CATG 1 cut(s) 553
FaiI YATR 6 cut(s) 143, 170, 212, 335, 537, 551
FatI CATG 1 cut(s) 549
FauI CCCGC 2 cut(s) 146, 399
FblI GTMKAC 1 cut(s) 536
Fnu4HI GCNGC 5 cut(s) 42, 76, 404, 429, 432
FokI GGATG 1 cut(s) 30
Fsp4HI GCNGC 5 cut(s) 42, 76, 404, 429, 432
FspBI CTAG 1 cut(s) 302
GluI GCNGC 5 cut(s) 42, 76, 404, 429, 432
HaeIII GGCC 2 cut(s) 157, 338
HapII CCGG 1 cut(s) 289
HgaI GACGC 2 cut(s) 394, 423
Hin1I GRCGYC 2 cut(s) 326, 386
Hin1II CATG 1 cut(s) 553
HinfI GANTC 3 cut(s) 50, 279, 376
HpaII CCGG 1 cut(s) 289
HphI GGTGA 4 cut(s) 190, 241, 347, 497
Hpy166II GTNNAC 1 cut(s) 537
Hpy188I TCNGA 3 cut(s) 55, 149, 384
Hpy188III TCNNGA 4 cut(s) 47, 59, 136, 236
Hpy8I GTNNAC 1 cut(s) 537
Hpy99I CGWCG 3 cut(s) 137, 311, 394
HpyAV CCTTC 1 cut(s) 263
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 2 cut(s) 326, 492
HpyCH4V TGCA 2 cut(s) 403, 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 1 cut(s) 528
HpySE526I ACGT 2 cut(s) 326, 492
Hsp92I GRCGYC 2 cut(s) 326, 386
Hsp92II CATG 1 cut(s) 553
Kzo9I GATC 3 cut(s) 29, 55, 174
LmnI GCTCC 1 cut(s) 224
Lsp1109I GCAGC 4 cut(s) 28, 415, 418, 440
LweI GCATC 3 cut(s) 8, 178, 415
MabI ACCWGGT 1 cut(s) 507
MaeI CTAG 1 cut(s) 302
MaeII ACGT 2 cut(s) 326, 492
MaeIII GTNAC 3 cut(s) 181, 466, 503
MalI GATC 3 cut(s) 31, 57, 176
MboI GATC 3 cut(s) 29, 55, 174
MboII GAAGA 4 cut(s) 39, 371, 470, 527
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 72
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 1 cut(s) 448
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 1 cut(s) 370
MmeI TCCRAC 2 cut(s) 62, 246
MnlI CCTC 4 cut(s) 202, 206, 251, 482
Mox20I TGGCCA 1 cut(s) 338
MscI TGGCCA 1 cut(s) 338
MseI TTAA 1 cut(s) 396
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 3 cut(s) 78, 406, 431
MspI CCGG 1 cut(s) 289
MspR9I CCNGG 1 cut(s) 509
MssI GTTTAAAC 1 cut(s) 397
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 1 cut(s) 344
NdeII GATC 3 cut(s) 29, 55, 174
NlaIII CATG 1 cut(s) 553
NmuCI GTSAC 1 cut(s) 503
NspI RCATGY 1 cut(s) 553
NspV TTCGAA 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PfeI GAWTC 2 cut(s) 50, 279
PflFI GACNNNGTC 2 cut(s) 388, 461
PinAI ACCGGT 1 cut(s) 288
PkrI GCNGC 5 cut(s) 43, 77, 405, 430, 433
Ple19I CGATCG 1 cut(s) 58
PleI GAGTC 1 cut(s) 370
PmeI GTTTAAAC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 370
Ppu21I YACGTR 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspPI GGNCC 1 cut(s) 155
PstI CTGCAG 1 cut(s) 405
PsuI RGATCY 1 cut(s) 29
PsyI GACNNNGTC 2 cut(s) 388, 461
PvuI CGATCG 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 3 cut(s) 293, 491, 495
RsaNI GTAC 3 cut(s) 292, 490, 494
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 5 cut(s) 42, 76, 404, 429, 432
Sau3AI GATC 3 cut(s) 29, 55, 174
Sau96I GGNCC 1 cut(s) 155
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 1 cut(s) 72
SetI ASST 6 cut(s) 221, 243, 329, 433, 495, 510
SexAI ACCWGGT 1 cut(s) 507
SfaNI GCATC 3 cut(s) 8, 178, 415
SfcI CTRYAG 1 cut(s) 401
SfuI TTCGAA 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 45
SmoI CTYRAG 1 cut(s) 45
SnaBI TACGTA 1 cut(s) 493
Sse9I AATT 1 cut(s) 448
SsiI CCGC 4 cut(s) 76, 153, 319, 406
SspI AATATT 1 cut(s) 415
SspMI CTAG 1 cut(s) 302
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 2 cut(s) 329, 495
TaqI TCGA 3 cut(s) 135, 237, 256
TasI AATT 1 cut(s) 448
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 2 cut(s) 50, 279
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 2 cut(s) 77, 184
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 4 cut(s) 41, 403, 428, 431
Tsp45I GTSAC 1 cut(s) 503
TspGWI ACGGA 1 cut(s) 491
TspRI CASTG 2 cut(s) 77, 184
Tth111I GACNNNGTC 2 cut(s) 388, 461
XceI RCATGY 1 cut(s) 553
XmiI GTMKAC 1 cut(s) 536
XspI CTAG 1 cut(s) 302
ZraI GACGTC 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.