RLG00000025916

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0010575
Physical Location & Seq
Reverse (-)
49161413 .. 49163910
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025916

Sequence Viewer

Length: 1086 bp
ATGAGTTTCTATATATGTACAGATTCCGACCAGGAAACCGAGCACTGCCCCTGTAGGATTATCTTACTCGCTGTGTTCCTTTTCCTTTTACTGCTGTTTTATATCGTCGAGAGCATAATCAGAAGCGAGCCACCAGATGACATAAAGATCACTGTAACTGATGCCTCCCTCACCGAGTTCAACATAACCAGCTCCAACAACTACAATCTCGACCTCAACATCACCCTTCGAAATTCCAACAAGGATTTTGGCATCTACTTCCGGTACAAGGCTCTAGTCGTCGCAGACTACCGCAAGACGTCTGCTATGGCCAGCATTGCTAACTTTCACCAACCAGCAAAGAATACGACTCTTCTGACGCCGTCGTTTAAACTGCAGCGGGATAATGTTACTGACGATGCAGCTGCGTCAGGGAAAGGGAATTACTACGACATTGTCGTCACCCTCTATCTTCCAAAGAAGTACAAGTCCGTCACCTGGTGGAAGAACGATTGGCTAAGCAGTATACAACTCTACGGATGTGACTTGAAGGTTCCTTTGAACAATGGATCAAAATCAGTCGATGCTTTCAATACTACCCACTGCGCGCCAAATAATGATTACCACCGTTGGTTTTTGGTTTTCAGAGTGACGGTCTTGGTAGACGACGCCATATCGATGAAATGGATTGAGGAGATTGGTGGGTTTGTAATTTTTACCGCGACCACCACCAACAATTTCAGTGCGACTATTCTTCACTACAAGCTTAATCTCAACATCGCAGTCGGAAACCCAAACGAAAACTACGACATCTACCACGACAAAGTTCAAATGCTGGCTTATTATAAGAAGCAGAGGTGTGGCATGGCGAATTTAAGCTCTTTTTACCAAGGGAAGAGGAACATAACTGAAATGACACTGCCGTTGAAAGGACAACAAGTGATGATTTTTGATGGGATTGAGGATCTTTGCAAGCTTAAGCATCGCGACGATGGAGCTGATTACGATTTTGTTGTTGAGCTCTATCGTCAAAACAAGTACCAAGCCACTGCGTCAGCCATGACTAGGTTGATTCGGCCGGGTTGGGAAGATAAGTCCAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

362

Amino Acids

41.48

Weight (kDa)

7.03

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 825
AasI GACNNNNNNGTC 1 cut(s) 437
AatII GACGTC 1 cut(s) 302
AccI GTMKAC 2 cut(s) 505, 642
AccII CGCG 3 cut(s) 587, 701, 966
AciI CCGC 3 cut(s) 292, 379, 699
AclWI GGATC 2 cut(s) 556, 951
AcoI YGGCCR 2 cut(s) 309, 1055
AcsI RAATTY 2 cut(s) 232, 850
AcyI GRCGYC 3 cut(s) 299, 359, 648
AdeI CACNNNGTG 1 cut(s) 480
AfaI GTAC 4 cut(s) 19, 266, 464, 1019
AfiI CCNNNNNNNGG 4 cut(s) 268, 477, 908, 1044
AflII CTTAAG 1 cut(s) 956
AgsI TTSAA 6 cut(s) 181, 529, 541, 571, 809, 907
AjnI CCWGG 2 cut(s) 30, 476
AluBI AGCT 7 cut(s) 192, 404, 745, 858, 955, 977, 1000
AluI AGCT 7 cut(s) 192, 404, 745, 858, 955, 977, 1000
Alw21I GWGCWC 2 cut(s) 45, 1002
AlwI GGATC 2 cut(s) 556, 951
AoxI GGCC 2 cut(s) 309, 1055
ApeKI GCWGC 3 cut(s) 376, 401, 404
ApoI RAATTY 2 cut(s) 232, 850
AspLEI GCGC 2 cut(s) 587, 589
AsuC2I CCSGG 1 cut(s) 1059
AsuHPI GGTGA 5 cut(s) 163, 214, 320, 433, 466
AsuII TTCGAA 1 cut(s) 229
BalI TGGCCA 1 cut(s) 311
BanII GRGCYC 1 cut(s) 1002
Bbv12I GWGCWC 2 cut(s) 45, 1002
BbvI GCAGC 3 cut(s) 388, 391, 413
BccI CCATC 2 cut(s) 926, 965
BceAI ACGGC 2 cut(s) 346, 886
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 2 cut(s) 32, 478
BcnI CCSGG 1 cut(s) 1059
BfaI CTAG 2 cut(s) 275, 1044
BfmI CTRYAG 2 cut(s) 52, 374
BfrI CTTAAG 1 cut(s) 956
BisI GCNGC 3 cut(s) 377, 402, 405
BlpI GCTNAGC 1 cut(s) 497
BlsI GCNGC 3 cut(s) 378, 403, 406
Bme1390I CCNGG 3 cut(s) 32, 478, 1059
BmiI GGNNCC 1 cut(s) 534
BmrFI CCNGG 3 cut(s) 32, 478, 1059
BmsI GCATC 5 cut(s) 151, 261, 388, 553, 970
Bpu1102I GCTNAGC 1 cut(s) 497
Bpu14I TTCGAA 1 cut(s) 229
BpuMI CCSGG 1 cut(s) 1059
Bsa29I ATCGAT 1 cut(s) 656
BsaBI GATNNNNATC 1 cut(s) 553
BsaHI GRCGYC 3 cut(s) 299, 359, 648
BsaJI CCNNGG 1 cut(s) 868
BsaWI WCCGGW 1 cut(s) 261
Bsc4I CCNNNNNNNGG 4 cut(s) 268, 477, 908, 1044
Bse3DI GCAATG 1 cut(s) 315
Bse8I GATNNNNATC 1 cut(s) 553
BseBI CCWGG 2 cut(s) 32, 478
BseCI ATCGAT 1 cut(s) 656
BseDI CCNNGG 1 cut(s) 868
BseGI GGATG 1 cut(s) 524
BseJI GATNNNNATC 1 cut(s) 553
BseLI CCNNNNNNNGG 4 cut(s) 268, 477, 908, 1044
BseMI GCAATG 1 cut(s) 315
BsePI GCGCGC 1 cut(s) 585
BseRI GAGGAG 1 cut(s) 686
BseX3I CGGCCG 1 cut(s) 1055
BseXI GCAGC 3 cut(s) 388, 391, 413
Bsh1236I CGCG 3 cut(s) 587, 701, 966
Bsh1285I CGRYCG 1 cut(s) 1058
BshFI GGCC 2 cut(s) 311, 1057
BshVI ATCGAT 1 cut(s) 656
BsiEI CGRYCG 1 cut(s) 1058
BsiHKAI GWGCWC 2 cut(s) 45, 1002
BsiSI CCGG 2 cut(s) 262, 1058
BslI CCNNNNNNNGG 4 cut(s) 268, 477, 908, 1044
BsnI GGCC 2 cut(s) 311, 1057
Bsp119I TTCGAA 1 cut(s) 229
Bsp1286I GDGCHC 2 cut(s) 45, 1002
Bsp1407I TGTACA 1 cut(s) 17
Bsp143I GATC 3 cut(s) 147, 548, 943
Bsp1720I GCTNAGC 1 cut(s) 497
Bsp68I TCGCGA 1 cut(s) 966
BspACI CCGC 3 cut(s) 292, 379, 699
BspANI GGCC 2 cut(s) 311, 1057
BspDI ATCGAT 1 cut(s) 656
BspFNI CGCG 3 cut(s) 587, 701, 966
BspLI GGNNCC 1 cut(s) 534
BspMAI CTGCAG 1 cut(s) 378
BspPI GGATC 2 cut(s) 556, 951
BspT104I TTCGAA 1 cut(s) 229
BspTI CTTAAG 1 cut(s) 956
BsrDI GCAATG 1 cut(s) 315
BsrGI TGTACA 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 868
BssHII GCGCGC 1 cut(s) 585
BssMI GATC 3 cut(s) 147, 548, 943
BssNAI GTATAC 1 cut(s) 506
BssNI GRCGYC 3 cut(s) 299, 359, 648
BssT1I CCWWGG 1 cut(s) 868
Bst1107I GTATAC 1 cut(s) 506
Bst2UI CCWGG 2 cut(s) 32, 478
Bst4CI ACNGT 3 cut(s) 154, 608, 634
Bst6I CTCTTC 2 cut(s) 357, 869
BstACI GRCGYC 3 cut(s) 299, 359, 648
BstAFI CTTAAG 1 cut(s) 956
BstAUI TGTACA 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 5 cut(s) 128, 313, 587, 816, 953
BstDEI CTNAG 1 cut(s) 497
BstF5I GGATG 1 cut(s) 524
BstFNI CGCG 3 cut(s) 587, 701, 966
BstHHI GCGC 2 cut(s) 587, 589
BstKTI GATC 3 cut(s) 150, 551, 946
BstMBI GATC 3 cut(s) 147, 548, 943
BstMCI CGRYCG 1 cut(s) 1058
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNI CCWGG 2 cut(s) 32, 478
BstSCI CCNGG 3 cut(s) 30, 476, 1057
BstSFI CTRYAG 2 cut(s) 52, 374
BstUI CGCG 3 cut(s) 587, 701, 966
BstV1I GCAGC 3 cut(s) 388, 391, 413
BstX2I RGATCY 1 cut(s) 943
BstYI RGATCY 1 cut(s) 943
BstZ17I GTATAC 1 cut(s) 506
BstZI CGGCCG 1 cut(s) 1055
Bsu15I ATCGAT 1 cut(s) 656
BsuRI GGCC 2 cut(s) 311, 1057
BsuTUI ATCGAT 1 cut(s) 656
BtgZI GCGATG 2 cut(s) 742, 947
BtsCI GGATG 1 cut(s) 524
BtsI GCAGTG 4 cut(s) 43, 580, 896, 1026
BtsIMutI CAGTG 6 cut(s) 43, 150, 580, 727, 896, 1026
BtuMI TCGCGA 1 cut(s) 966
Cac8I GCNNGC 5 cut(s) 128, 313, 587, 816, 953
CfoI GCGC 2 cut(s) 587, 589
ClaI ATCGAT 1 cut(s) 656
CseI GACGC 4 cut(s) 367, 396, 656, 1020
CsiI ACCWGGT 1 cut(s) 476
Csp6I GTAC 4 cut(s) 18, 265, 463, 1018
CviAII CATG 2 cut(s) 844, 1039
CviQI GTAC 4 cut(s) 18, 265, 463, 1018
DdeI CTNAG 1 cut(s) 497
DpnI GATC 3 cut(s) 149, 550, 945
DpnII GATC 3 cut(s) 147, 548, 943
DraI TTTAAA 1 cut(s) 370
DraIII CACNNNGTG 1 cut(s) 480
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 2 cut(s) 309, 1055
EagI CGGCCG 1 cut(s) 1055
Eam1104I CTCTTC 2 cut(s) 357, 869
EarI CTCTTC 2 cut(s) 357, 869
Ecl136II GAGCTC 1 cut(s) 1000
EclXI CGGCCG 1 cut(s) 1055
Eco130I CCWWGG 1 cut(s) 868
Eco24I GRGCYC 1 cut(s) 1002
Eco52I CGGCCG 1 cut(s) 1055
Eco53kI GAGCTC 1 cut(s) 1000
EcoICRI GAGCTC 1 cut(s) 1000
EcoRII CCWGG 2 cut(s) 30, 476
EcoT14I CCWWGG 1 cut(s) 868
EcoT38I GRGCYC 1 cut(s) 1002
ErhI CCWWGG 1 cut(s) 868
FaeI CATG 2 cut(s) 847, 1042
FatI CATG 2 cut(s) 843, 1038
FauI CCCGC 1 cut(s) 372
FblI GTMKAC 2 cut(s) 505, 642
Fnu4HI GCNGC 3 cut(s) 377, 402, 405
FokI GGATG 1 cut(s) 531
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 3 cut(s) 377, 402, 405
FspBI CTAG 2 cut(s) 275, 1044
GlaI GCGC 2 cut(s) 586, 588
GluI GCNGC 3 cut(s) 377, 402, 405
HaeIII GGCC 2 cut(s) 311, 1057
HapII CCGG 2 cut(s) 262, 1058
HgaI GACGC 4 cut(s) 367, 396, 656, 1020
HhaI GCGC 2 cut(s) 587, 589
Hin1I GRCGYC 3 cut(s) 299, 359, 648
Hin1II CATG 2 cut(s) 847, 1042
Hin6I GCGC 2 cut(s) 585, 587
HinP1I GCGC 2 cut(s) 585, 587
HindIII AAGCTT 2 cut(s) 743, 953
HinfI GANTC 3 cut(s) 23, 349, 1051
HpaII CCGG 2 cut(s) 262, 1058
HphI GGTGA 5 cut(s) 163, 214, 320, 433, 466
Hpy166II GTNNAC 2 cut(s) 506, 643
Hpy188I TCNGA 5 cut(s) 28, 122, 357, 626, 767
Hpy188III TCNNGA 3 cut(s) 109, 209, 965
Hpy8I GTNNAC 2 cut(s) 506, 643
Hpy99I CGWCG 5 cut(s) 110, 284, 367, 650, 971
HpyAV CCTTC 2 cut(s) 236, 523
HpyCH4III ACNGT 3 cut(s) 154, 608, 634
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 3 cut(s) 376, 401, 951
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 1 cut(s) 497
HpySE526I ACGT 1 cut(s) 299
Hsp92I GRCGYC 3 cut(s) 299, 359, 648
Hsp92II CATG 2 cut(s) 847, 1042
HspAI GCGC 2 cut(s) 585, 587
Kzo9I GATC 3 cut(s) 147, 548, 943
LmnI GCTCC 2 cut(s) 197, 974
Lsp1109I GCAGC 3 cut(s) 388, 391, 413
LweI GCATC 5 cut(s) 151, 261, 388, 553, 970
MabI ACCWGGT 1 cut(s) 476
MaeI CTAG 2 cut(s) 275, 1044
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 6 cut(s) 154, 388, 439, 472, 521, 628
MalI GATC 3 cut(s) 149, 550, 945
MboI GATC 3 cut(s) 147, 548, 943
MboII GAAGA 6 cut(s) 344, 443, 496, 725, 886, 1079
MflI RGATCY 1 cut(s) 943
MhlI GDGCHC 2 cut(s) 45, 1002
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 5 cut(s) 232, 421, 690, 715, 850
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 1 cut(s) 343
MmeI TCCRAC 4 cut(s) 51, 219, 261, 745
MnlI CCTC 8 cut(s) 175, 179, 224, 455, 664, 828, 870, 934
Mox20I TGGCCA 1 cut(s) 311
MscI TGGCCA 1 cut(s) 311
MseI TTAA 4 cut(s) 369, 747, 854, 957
MslI CAYNNNNRTG 1 cut(s) 656
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 2 cut(s) 379, 404
MspCI CTTAAG 1 cut(s) 956
MspI CCGG 2 cut(s) 262, 1058
MspR9I CCNGG 3 cut(s) 32, 478, 1059
MssI GTTTAAAC 1 cut(s) 370
MvaI CCWGG 2 cut(s) 32, 478
MvnI CGCG 3 cut(s) 587, 701, 966
MwoI GCNNNNNNNGC 1 cut(s) 317
NciI CCSGG 1 cut(s) 1059
NdeII GATC 3 cut(s) 147, 548, 943
NlaIII CATG 2 cut(s) 847, 1042
NlaIV GGNNCC 1 cut(s) 534
NmuCI GTSAC 4 cut(s) 439, 472, 521, 628
NruI TCGCGA 1 cut(s) 966
NspV TTCGAA 1 cut(s) 229
PauI GCGCGC 1 cut(s) 585
PcsI WCGNNNNNNNCGW 2 cut(s) 435, 783
PfeI GAWTC 2 cut(s) 23, 1051
PflFI GACNNNGTC 2 cut(s) 361, 434
PkrI GCNGC 3 cut(s) 378, 403, 406
PleI GAGTC 1 cut(s) 343
PmeI GTTTAAAC 1 cut(s) 370
PpsI GAGTC 1 cut(s) 343
PsiI TTATAA 1 cut(s) 825
Psp124BI GAGCTC 1 cut(s) 1002
Psp6I CCWGG 2 cut(s) 30, 476
PspGI CCWGG 2 cut(s) 30, 476
PspN4I GGNNCC 1 cut(s) 534
PstI CTGCAG 1 cut(s) 378
PsuI RGATCY 1 cut(s) 943
PsyI GACNNNGTC 2 cut(s) 361, 434
PteI GCGCGC 1 cut(s) 585
PvuII CAGCTG 1 cut(s) 404
RruI TCGCGA 1 cut(s) 966
RsaI GTAC 4 cut(s) 19, 266, 464, 1019
RsaNI GTAC 4 cut(s) 18, 265, 463, 1018
RseI CAYNNNNRTG 1 cut(s) 656
SacI GAGCTC 1 cut(s) 1002
SaqAI TTAA 4 cut(s) 369, 747, 854, 957
SatI GCNGC 3 cut(s) 377, 402, 405
Sau3AI GATC 3 cut(s) 147, 548, 943
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 3 cut(s) 32, 478, 1059
SduI GDGCHC 2 cut(s) 45, 1002
SexAI ACCWGGT 1 cut(s) 476
SfaNI GCATC 5 cut(s) 151, 261, 388, 553, 970
SfcI CTRYAG 2 cut(s) 52, 374
SfuI TTCGAA 1 cut(s) 229
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
Sse9I AATT 5 cut(s) 232, 421, 690, 715, 850
SsiI CCGC 3 cut(s) 292, 379, 699
SspMI CTAG 2 cut(s) 275, 1044
SstI GAGCTC 1 cut(s) 1002
StyD4I CCNGG 3 cut(s) 30, 476, 1057
StyI CCWWGG 1 cut(s) 868
TaaI ACNGT 3 cut(s) 154, 608, 634
TaiI ACGT 1 cut(s) 302
TaqI TCGA 5 cut(s) 108, 210, 229, 561, 656
TasI AATT 5 cut(s) 232, 421, 690, 715, 850
TatI WGTACW 2 cut(s) 17, 462
TfiI GAWTC 2 cut(s) 23, 1051
Tru1I TTAA 4 cut(s) 369, 747, 854, 957
Tru9I TTAA 4 cut(s) 369, 747, 854, 957
TscAI CASTG 6 cut(s) 50, 157, 587, 727, 903, 1033
TseFI GTSAC 4 cut(s) 439, 472, 521, 628
TseI GCWGC 3 cut(s) 376, 401, 404
Tsp45I GTSAC 4 cut(s) 439, 472, 521, 628
TspDTI ATGAA 1 cut(s) 674
TspGWI ACGGA 2 cut(s) 460, 531
TspRI CASTG 6 cut(s) 50, 157, 587, 727, 903, 1033
Tth111I GACNNNGTC 2 cut(s) 361, 434
Vha464I CTTAAG 1 cut(s) 956
XapI RAATTY 2 cut(s) 232, 850
XmiI GTMKAC 2 cut(s) 505, 642
XspI CTAG 2 cut(s) 275, 1044
ZraI GACGTC 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.