FvH4_6g02660
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011183
Physical Location & Seq
Reverse (-)
1520191 .. 1522333
2143 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02660.t1

Sequence Viewer

Length: 960 bp
ATGGAGGAGGTTGAAGCAGCTAACAAAGCAGCAGTAGAGAGTTGCCATAGAGTTCTCAACAGTCTCATTTGCAAACACAAAGATGATGTTCAGTGTGAAAATTTAGGTGTGCTTACAGAAGAGGCCGTTGTGAAGTTCAAGAGAGTTGTGTCTCTTCTGAATCATGGAAGGATCAGAAAGTTGAAGAAGAAGCTTGACTCATCTTGGCCTCAAAGCCTCATGTTGGATGGCCCTAATTACAGATCAGACATTTCAACCAAAACCCTCCAATTACTCCCAGCTACTTTCCCTCCTAACCCACACACACAAAACCAGAAAGTGATTCTAGGAAATCCAGTGTTGGATATGAGTCTTAATCTTCCTCTCCAAAATGTTCAACCAAAACCATTTCAGCAGTTGCATAGAATTCCAGTCCATCCCCAGCAGTTCACTGGCATAAACCTTGCATTTAACAAGCCTAGCACCACACCCTCCTCGTCCAACGCTGCGTCTTTCATGTCATGTTTGAGCATGGATGCTGGTCGTGTGGCTAACTATGACAGCAATTCCTTCAGACTAATCAGTGGCTGCGTGCCGCAGCCGATGGATCAAATATCGCAACTGATGAGGAGCGCTGATGTACAGCAGAACAGCAGTGTACAATGTGGAAGCAGTGAGAAGTGTTCCACACCAAGGAAGAGGAGATTGAGGGTGAGAAAATCCTTCAAGGTACCTGAAGGAGGTAAGAATGTAGCACACATTCCAGGTGATGAATATTCATGGAGGAAGTATGGACAGAAGCCAATCAAGGGCTCTCCTTATCCAAGGGGATACTATAAGTGTAGCAGCATGAGAGGCTGCCCAGCAAGGAAACATGTCGAGAGGTGCATCGAAGACCCTGCAATGCTGATAGTCACCTATGAGAGCGAGCACAAGCATTCACAATCTGCCCACAAGCATTCACAATCTGCCCATCAATGA

Protein Analysis

320

Amino Acids

35.77

Weight (kDa)

9.58

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 250 - 306 2.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04670 AT3G04670 AT5G28650
fragaria_vesca FvH4_6g02660 FvH4_6g02660
malus_domestica MD04G1226400.v1.1 MD12G1243400.v1.1
prunus_persica Prupe.6G345100_v2.0.a1
pyrus_communis pycom04g19960 pycom12g22250
rosa_chinensis RchiOBHm_Chr3g0450591
rosa_laevigata RLG00000025718
rosa_multiflora Rmu_sc0000078.1_g000042
rosa_roxburghii Rroxscaffold_6G00428290
rosa_rugosa Rorug02G0629000
rosa_samantha Rh3AG029700 Rh3BG030100 Rh3CG029000 Rh3DG029800
rosa_wichuraiana Rw3G002290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 709
AccB1I GGYRCC 1 cut(s) 709
AciI CCGC 1 cut(s) 575
AclWI GGATC 2 cut(s) 179, 594
AcsI RAATTY 2 cut(s) 100, 405
AcuI CTGAAG 2 cut(s) 535, 735
AfaI GTAC 3 cut(s) 621, 639, 711
AfeI AGCGCT 1 cut(s) 613
AfiI CCNNNNNNNGG 4 cut(s) 223, 672, 719, 788
AflIII ACRYGT 1 cut(s) 853
AgsI TTSAA 6 cut(s) 14, 139, 184, 255, 377, 706
AjnI CCWGG 1 cut(s) 742
AluBI AGCT 3 cut(s) 20, 193, 281
AluI AGCT 3 cut(s) 20, 193, 281
Alw21I GWGCWC 1 cut(s) 912
Alw26I GTCTC 2 cut(s) 68, 156
AlwI GGATC 2 cut(s) 179, 594
AlwNI CAGNNNCTG 1 cut(s) 567
Aor51HI AGCGCT 1 cut(s) 613
AoxI GGCC 3 cut(s) 123, 206, 229
ApeKI GCWGC 7 cut(s) 17, 29, 485, 567, 577, 825, 837
ApoI RAATTY 2 cut(s) 100, 405
Asp718I GGTACC 1 cut(s) 709
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 1 cut(s) 230
AsuHPI GGTGA 3 cut(s) 703, 758, 886
BanI GGYRCC 1 cut(s) 709
BanII GRGCYC 1 cut(s) 794
BbsI GAAGAC 1 cut(s) 879
Bbv12I GWGCWC 1 cut(s) 912
BbvI GCAGC 7 cut(s) 29, 41, 472, 554, 589, 824, 837
BccI CCATC 3 cut(s) 221, 423, 577
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 860, 894
BciT130I CCWGG 1 cut(s) 744
BciVI GTATCC 1 cut(s) 803
BcoDI GTCTC 2 cut(s) 68, 156
BfaI CTAG 2 cut(s) 326, 459
BfoI RGCGCY 1 cut(s) 615
BfuI GTATCC 1 cut(s) 803
BisI GCNGC 8 cut(s) 18, 30, 486, 568, 575, 578, 826, 838
BlsI GCNGC 8 cut(s) 19, 31, 487, 569, 576, 579, 827, 839
Bme1390I CCNGG 1 cut(s) 744
BmgT120I GGNCC 1 cut(s) 230
BmiI GGNNCC 1 cut(s) 711
BmrFI CCNGG 1 cut(s) 744
BmsI GCATC 2 cut(s) 505, 876
BpiI GAAGAC 1 cut(s) 879
BsaJI CCNNGG 2 cut(s) 671, 803
BsaXI ACNNNNNCTCC 2 cut(s) 274, 304
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 672, 719, 788
Bse1I ACTGG 3 cut(s) 335, 410, 436
Bse3DI GCAATG 1 cut(s) 888
BseBI CCWGG 1 cut(s) 744
BseDI CCNNGG 2 cut(s) 671, 803
BseGI GGATG 3 cut(s) 232, 415, 520
BseLI CCNNNNNNNGG 4 cut(s) 223, 672, 719, 788
BseMI GCAATG 1 cut(s) 888
BseNI ACTGG 3 cut(s) 335, 410, 436
BseRI GAGGAG 4 cut(s) 20, 463, 622, 694
BseXI GCAGC 7 cut(s) 29, 41, 472, 554, 589, 824, 837
BseYI CCCAGC 3 cut(s) 277, 420, 841
BshFI GGCC 3 cut(s) 125, 208, 231
BshNI GGYRCC 1 cut(s) 709
BsiHKAI GWGCWC 1 cut(s) 912
BslI CCNNNNNNNGG 4 cut(s) 223, 672, 719, 788
BsmAI GTCTC 2 cut(s) 68, 156
BsmI GAATGC 2 cut(s) 916, 937
BsnI GGCC 3 cut(s) 125, 208, 231
Bsp1286I GDGCHC 2 cut(s) 794, 912
Bsp1407I TGTACA 2 cut(s) 619, 637
Bsp143I GATC 3 cut(s) 171, 242, 586
BspACI CCGC 1 cut(s) 575
BspANI GGCC 3 cut(s) 125, 208, 231
BspLI GGNNCC 1 cut(s) 711
BspPI GGATC 2 cut(s) 179, 594
BspT107I GGYRCC 1 cut(s) 709
BsrDI GCAATG 1 cut(s) 888
BsrGI TGTACA 2 cut(s) 619, 637
BsrI ACTGG 3 cut(s) 335, 410, 436
BssECI CCNNGG 2 cut(s) 671, 803
BssMI GATC 3 cut(s) 171, 242, 586
BssT1I CCWWGG 2 cut(s) 671, 803
Bst2UI CCWGG 1 cut(s) 744
Bst4CI ACNGT 1 cut(s) 62
Bst6I CTCTTC 3 cut(s) 114, 159, 671
BstAUI TGTACA 2 cut(s) 619, 637
BstC8I GCNNGC 2 cut(s) 572, 908
BstENI CCTNNNNNAGG 1 cut(s) 717
BstF5I GGATG 3 cut(s) 232, 415, 520
BstH2I RGCGCY 1 cut(s) 615
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 3 cut(s) 174, 245, 589
BstMAI GTCTC 2 cut(s) 68, 156
BstMBI GATC 3 cut(s) 171, 242, 586
BstMWI GCNNNNNNNGC 2 cut(s) 26, 834
BstNI CCWGG 1 cut(s) 744
BstNSI RCATGY 1 cut(s) 857
BstSCI CCNGG 1 cut(s) 742
BstV1I GCAGC 7 cut(s) 29, 41, 472, 554, 589, 824, 837
BstV2I GAAGAC 1 cut(s) 879
BsuI GTATCC 1 cut(s) 803
BsuRI GGCC 3 cut(s) 125, 208, 231
BtsCI GGATG 3 cut(s) 232, 415, 520
BtsI GCAGTG 2 cut(s) 640, 658
BtsIMutI CAGTG 6 cut(s) 98, 342, 429, 568, 640, 658
Cac8I GCNNGC 2 cut(s) 572, 908
CaiI CAGNNNCTG 1 cut(s) 567
CfoI GCGC 1 cut(s) 614
Cfr13I GGNCC 1 cut(s) 230
CseI GACGC 1 cut(s) 477
Csp6I GTAC 3 cut(s) 620, 638, 710
CviAII CATG 8 cut(s) 164, 220, 496, 501, 511, 759, 829, 854
CviQI GTAC 3 cut(s) 620, 638, 710
DpnI GATC 3 cut(s) 173, 244, 588
DpnII GATC 3 cut(s) 171, 242, 586
Eam1104I CTCTTC 3 cut(s) 114, 159, 671
EarI CTCTTC 3 cut(s) 114, 159, 671
Eco130I CCWWGG 2 cut(s) 671, 803
Eco24I GRGCYC 1 cut(s) 794
Eco47III AGCGCT 1 cut(s) 613
Eco57I CTGAAG 2 cut(s) 535, 735
EcoNI CCTNNNNNAGG 1 cut(s) 717
EcoRI GAATTC 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 742
EcoT14I CCWWGG 2 cut(s) 671, 803
EcoT38I GRGCYC 1 cut(s) 794
ErhI CCWWGG 2 cut(s) 671, 803
FaeI CATG 8 cut(s) 167, 223, 499, 504, 514, 762, 832, 857
FatI CATG 8 cut(s) 163, 219, 495, 500, 510, 758, 828, 853
Fnu4HI GCNGC 8 cut(s) 18, 30, 486, 568, 575, 578, 826, 838
FokI GGATG 3 cut(s) 239, 402, 527
FriOI GRGCYC 1 cut(s) 794
Fsp4HI GCNGC 8 cut(s) 18, 30, 486, 568, 575, 578, 826, 838
FspBI CTAG 2 cut(s) 326, 459
GlaI GCGC 1 cut(s) 613
GluI GCNGC 8 cut(s) 18, 30, 486, 568, 575, 578, 826, 838
GsaI CCCAGC 3 cut(s) 281, 424, 845
HaeII RGCGCY 1 cut(s) 615
HaeIII GGCC 3 cut(s) 125, 208, 231
HgaI GACGC 1 cut(s) 477
HhaI GCGC 1 cut(s) 614
Hin1II CATG 8 cut(s) 167, 223, 499, 504, 514, 762, 832, 857
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 4 cut(s) 160, 197, 322, 349
HphI GGTGA 3 cut(s) 703, 758, 886
Hpy166II GTNNAC 2 cut(s) 429, 638
Hpy188I TCNGA 4 cut(s) 159, 176, 247, 554
Hpy188III TCNNGA 2 cut(s) 139, 859
Hpy8I GTNNAC 2 cut(s) 429, 638
HpyAV CCTTC 4 cut(s) 162, 559, 710, 712
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4V TGCA 5 cut(s) 72, 400, 446, 867, 881
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 834
Hsp92II CATG 8 cut(s) 167, 223, 499, 504, 514, 762, 832, 857
HspAI GCGC 1 cut(s) 612
KpnI GGTACC 1 cut(s) 713
Kzo9I GATC 3 cut(s) 171, 242, 586
LmnI GCTCC 1 cut(s) 609
Lsp1109I GCAGC 7 cut(s) 29, 41, 472, 554, 589, 824, 837
LweI GCATC 2 cut(s) 505, 876
MaeI CTAG 2 cut(s) 326, 459
MaeIII GTNAC 1 cut(s) 892
MalI GATC 3 cut(s) 173, 244, 588
MboI GATC 3 cut(s) 171, 242, 586
MboII GAAGA 7 cut(s) 131, 146, 196, 199, 350, 688, 884
MhlI GDGCHC 2 cut(s) 794, 912
MluCI AATT 5 cut(s) 100, 235, 269, 405, 544
MlyI GAGTC 2 cut(s) 191, 358
MmeI TCCRAC 3 cut(s) 204, 321, 504
MseI TTAA 2 cut(s) 354, 450
MslI CAYNNNNRTG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 744
Mva1269I GAATGC 2 cut(s) 916, 937
MvaI CCWGG 1 cut(s) 744
MwoI GCNNNNNNNGC 2 cut(s) 26, 834
NdeII GATC 3 cut(s) 171, 242, 586
NlaIII CATG 8 cut(s) 167, 223, 499, 504, 514, 762, 832, 857
NlaIV GGNNCC 1 cut(s) 711
NmuCI GTSAC 1 cut(s) 892
NspI RCATGY 1 cut(s) 857
PciI ACATGT 1 cut(s) 853
PctI GAATGC 2 cut(s) 916, 937
PfeI GAWTC 2 cut(s) 160, 322
PkrI GCNGC 8 cut(s) 19, 31, 487, 569, 576, 579, 827, 839
PleI GAGTC 2 cut(s) 191, 357
PpsI GAGTC 2 cut(s) 191, 357
PscI ACATGT 1 cut(s) 853
Psp6I CCWGG 1 cut(s) 742
PspFI CCCAGC 3 cut(s) 277, 420, 841
PspGI CCWGG 1 cut(s) 742
PspN4I GGNNCC 1 cut(s) 711
PspPI GGNCC 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 567
RsaI GTAC 3 cut(s) 621, 639, 711
RsaNI GTAC 3 cut(s) 620, 638, 710
RseI CAYNNNNRTG 1 cut(s) 81
SaqAI TTAA 2 cut(s) 354, 450
SatI GCNGC 8 cut(s) 18, 30, 486, 568, 575, 578, 826, 838
Sau3AI GATC 3 cut(s) 171, 242, 586
Sau96I GGNCC 1 cut(s) 230
SchI GAGTC 2 cut(s) 191, 358
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 2 cut(s) 794, 912
SfaNI GCATC 2 cut(s) 505, 876
SmiMI CAYNNNNRTG 1 cut(s) 81
Sse9I AATT 5 cut(s) 100, 235, 269, 405, 544
SsiI CCGC 1 cut(s) 575
SspI AATATT 1 cut(s) 755
SspMI CTAG 2 cut(s) 326, 459
StyD4I CCNGG 1 cut(s) 742
StyI CCWWGG 2 cut(s) 671, 803
TaaI ACNGT 1 cut(s) 62
TaqI TCGA 2 cut(s) 858, 870
TasI AATT 5 cut(s) 100, 235, 269, 405, 544
TatI WGTACW 2 cut(s) 619, 637
TauI GCSGC 1 cut(s) 577
TfiI GAWTC 2 cut(s) 160, 322
Tru1I TTAA 2 cut(s) 354, 450
Tru9I TTAA 2 cut(s) 354, 450
TscAI CASTG 6 cut(s) 98, 342, 436, 568, 640, 658
TseFI GTSAC 1 cut(s) 892
TseI GCWGC 7 cut(s) 17, 29, 485, 567, 577, 825, 837
Tsp45I GTSAC 1 cut(s) 892
TspDTI ATGAA 3 cut(s) 484, 747, 765
TspRI CASTG 6 cut(s) 98, 342, 436, 568, 640, 658
XagI CCTNNNNNAGG 1 cut(s) 717
XapI RAATTY 2 cut(s) 100, 405
XceI RCATGY 1 cut(s) 857
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XspI CTAG 2 cut(s) 326, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.