Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011183

Physical Location & Seq

Genomic Coordinates Forward (+)

31414877 .. 31416020

Gene Structure 1144 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1243400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 942 bp

ATGGAGGAGGTTGAGGCAGCTAACAAAGCAGCAGTTGAGAGCTGCCACAGAGTTCTGAATCTCTTGTGGAAACCAAAACCATGTAAACATTTGCAGGCGGAGACTGAGGTGGCAGTGTTCAAGTTCAAGAGAGTTCTCTCTCTCCTCAGCCATGGAAGAGGAAGAGTGAGAAAGTTGAAGAAAAACTTAAATCCACTACCTCTCCCTCACAATATCTTCTTAGATGGCCCTAATTATTCAGATATTTCACCAAAACCCCTCCAGTTACTCCCTCCTACTTTCCCTAAAACCCCTCACAAAGAAAAAGTTTTTCTTGGAAGCCCAGTTCCCAAAACAAAAACTTTGCAACAGCACCAGGAAGAGAGAACAAAATTACAGTTTCACCCCCAGCGGGTGAAATATAGGGATGAAATGGTGTTCTCTGGGAGAAACAGTGGCATCAACCTTGCATTTGATAGGTCTAGCTCTAGCTGCAAAACCTCCACGACGTCGGCCAGATCGTTCATGTCATGTTTGAGCATAGGCGGCTGTGCCGCCACCACCGGCAGAGATTCCTTTCGGTTAATAGGTGGTGTGCCTCGAGTTCCCGGTCAGATTTCCCAGCAGCAAAGGAGGTGCGTTGGTGTGGTGGAAAATGGGAGTGCGGAATGTCGCAGCAGTGGCAAATGCCATTTTCCAAAGAAGAGGAAACTGAAGGAGAAAAGATCTTATAAGGTCCCTGCCATTAGTGACAAGATTTCAGACATTCCATCCGATGAGTATTCGTGGAGGAAGTACGGGCAGAAGCCGATCAAGGGCTCCTTGTATCCGAGGGGATGCTACAAATGCAGCAGCATAAGAGGCTGTCCTGCGAGGAAACGTGTAGAGAGATGCCTTGAAGACCCTTCGATGCTCGTAGTCACCTACCAAGGCGAGCATAAGCATTCACAATCGGCCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Plant_zn_clust WRKY

Protein Analysis

314

Amino Acids

35.3

Weight (kDa)

9.96

Isoelectric Point (pI)

57.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Plant_zn_clust	PF10533	204 - 248	6.4e-11	Plant zinc cluster domain
WRKY	PF03106	252 - 308	5.1e-24	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011183)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04670 AT3G04670 AT5G28650
fragaria_vesca	FvH4_6g02660 FvH4_6g02660
malus_domestica	MD04G1226400.v1.1 MD12G1243400.v1.1
prunus_persica	Prupe.6G345100_v2.0.a1
pyrus_communis	pycom04g19960 pycom12g22250
rosa_chinensis	RchiOBHm_Chr3g0450591
rosa_laevigata	RLG00000025718
rosa_multiflora	Rmu_sc0000078.1_g000042
rosa_roxburghii	Rroxscaffold_6G00428290
rosa_rugosa	Rorug02G0629000
rosa_samantha	Rh3AG029700 Rh3BG030100 Rh3CG029000 Rh3DG029800
rosa_wichuraiana	Rw3G002290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	711
AatII	GACGTC	1 cut(s)	491
AciI	CCGC	5 cut(s)	98, 391, 525, 534, 644
AcoI	YGGCCR	1 cut(s)	492
AcuI	CTGAAG	1 cut(s)	713
AcyI	GRCGYC	1 cut(s)	488
AfaI	GTAC	1 cut(s)	776
AfiI	CCNNNNNNNGG	2 cut(s)	391, 794
AflIII	ACRYGT	1 cut(s)	859
AgsI	TTSAA	4 cut(s)	121, 127, 178, 878
AjnI	CCWGG	2 cut(s)	354, 935
AloI	GAACNNNNNNTCC	2 cut(s)	309, 341
AluBI	AGCT	4 cut(s)	20, 42, 465, 471
AluI	AGCT	4 cut(s)	20, 42, 465, 471
Alw26I	GTCTC	1 cut(s)	95
Ama87I	CYCGRG	1 cut(s)	579
AoxI	GGCC	3 cut(s)	226, 492, 933
ApeKI	GCWGC	8 cut(s)	17, 29, 42, 471, 604, 654, 828, 831
AspS9I	GGNCC	2 cut(s)	227, 715
AsuC2I	CCSGG	1 cut(s)	588
AsuHPI	GGTGA	4 cut(s)	240, 374, 406, 892
AvaI	CYCGRG	1 cut(s)	579
AvaII	GGWCC	1 cut(s)	715
BanII	GRGCYC	1 cut(s)	800
BbsI	GAAGAC	1 cut(s)	885
BbvCI	CCTCAGC	1 cut(s)	146
BbvI	GCAGC	8 cut(s)	29, 29, 41, 458, 616, 666, 840, 843
BccI	CCATC	2 cut(s)	218, 757
BciT130I	CCWGG	2 cut(s)	356, 937
BciVI	GTATCC	1 cut(s)	816
BcnI	CCSGG	1 cut(s)	588
BcoDI	GTCTC	1 cut(s)	95
BfaI	CTAG	2 cut(s)	462, 468
BfuI	GTATCC	1 cut(s)	816
BglII	AGATCT	1 cut(s)	704
Bme1390I	CCNGG	3 cut(s)	356, 588, 937
Bme18I	GGWCC	1 cut(s)	715
BmeT110I	CYCGRG	1 cut(s)	579
BmgT120I	GGNCC	2 cut(s)	227, 715
BmiI	GGNNCC	2 cut(s)	717, 799
BmrFI	CCNGG	3 cut(s)	356, 588, 937
BmrI	ACTGGG	1 cut(s)	317
BmsI	GCATC	4 cut(s)	447, 806, 860, 879
BmuI	ACTGGG	1 cut(s)	317
BpiI	GAAGAC	1 cut(s)	885
BpmI	CTGGAG	1 cut(s)	245
Bpu10I	CCTNAGC	1 cut(s)	146
BpuMI	CCSGG	1 cut(s)	588
BsaHI	GRCGYC	1 cut(s)	488
BsaJI	CCNNGG	3 cut(s)	151, 809, 907
BsaXI	ACNNNNNCTCC	4 cut(s)	186, 216, 418, 448
Bsc4I	CCNNNNNNNGG	2 cut(s)	391, 794
Bse118I	RCCGGY	1 cut(s)	542
Bse1I	ACTGG	2 cut(s)	262, 323
BseBI	CCWGG	2 cut(s)	356, 937
BseDI	CCNNGG	3 cut(s)	151, 809, 907
BseGI	GGATG	3 cut(s)	412, 749, 821
BseLI	CCNNNNNNNGG	2 cut(s)	391, 794
BseMII	CTCAG	2 cut(s)	96, 160
BseNI	ACTGG	2 cut(s)	262, 323
BseRI	GAGGAG	2 cut(s)	20, 134
BseXI	GCAGC	8 cut(s)	29, 29, 41, 458, 616, 666, 840, 843
BseYI	CCCAGC	2 cut(s)	387, 600
BshFI	GGCC	3 cut(s)	228, 494, 935
BsiHKCI	CYCGRG	1 cut(s)	579
BsiSI	CCGG	2 cut(s)	543, 588
BslFI	GGGAC	1 cut(s)	701
BslI	CCNNNNNNNGG	2 cut(s)	391, 794
BsmAI	GTCTC	1 cut(s)	95
BsmFI	GGGAC	1 cut(s)	701
BsmI	GAATGC	1 cut(s)	922
BsnI	GGCC	3 cut(s)	228, 494, 935
BsoBI	CYCGRG	1 cut(s)	579
Bsp1286I	GDGCHC	1 cut(s)	800
Bsp143I	GATC	3 cut(s)	497, 704, 789
Bsp19I	CCATGG	1 cut(s)	151
BspACI	CCGC	5 cut(s)	98, 391, 525, 534, 644
BspANI	GGCC	3 cut(s)	228, 494, 935
BspCNI	CTCAG	2 cut(s)	97, 159
BspLI	GGNNCC	2 cut(s)	717, 799
BsrFI	RCCGGY	1 cut(s)	542
BsrI	ACTGG	2 cut(s)	262, 323
BssAI	RCCGGY	1 cut(s)	542
BssECI	CCNNGG	3 cut(s)	151, 809, 907
BssMI	GATC	3 cut(s)	497, 704, 789
BssNI	GRCGYC	1 cut(s)	488
BssT1I	CCWWGG	2 cut(s)	151, 907
Bst2UI	CCWGG	2 cut(s)	356, 937
Bst4CI	ACNGT	2 cut(s)	378, 434
Bst6I	CTCTTC	4 cut(s)	151, 157, 354, 677
BstACI	GRCGYC	1 cut(s)	488
BstC8I	GCNNGC	2 cut(s)	96, 914
BstDEI	CTNAG	3 cut(s)	105, 146, 220
BstDSI	CCRYGG	1 cut(s)	151
BstF5I	GGATG	3 cut(s)	412, 749, 821
BstKTI	GATC	3 cut(s)	500, 707, 792
BstMAI	GTCTC	1 cut(s)	95
BstMBI	GATC	3 cut(s)	497, 704, 789
BstMWI	GCNNNNNNNGC	6 cut(s)	26, 471, 525, 660, 825, 840
BstNI	CCWGG	2 cut(s)	356, 937
BstSCI	CCNGG	3 cut(s)	354, 586, 935
BstV1I	GCAGC	8 cut(s)	29, 29, 41, 458, 616, 666, 840, 843
BstV2I	GAAGAC	1 cut(s)	885
BstX2I	RGATCY	1 cut(s)	704
BstYI	RGATCY	1 cut(s)	704
BsuI	GTATCC	1 cut(s)	816
BsuRI	GGCC	3 cut(s)	228, 494, 935
BtgI	CCRYGG	1 cut(s)	151
BtsCI	GGATG	3 cut(s)	412, 749, 821
BtsI	GCAGTG	2 cut(s)	120, 664
BtsIMutI	CAGTG	3 cut(s)	120, 439, 664
Cac8I	GCNNGC	2 cut(s)	96, 914
Cfr10I	RCCGGY	1 cut(s)	542
Cfr13I	GGNCC	2 cut(s)	227, 715
Csp6I	GTAC	1 cut(s)	775
CviAII	CATG	4 cut(s)	81, 152, 505, 510
CviQI	GTAC	1 cut(s)	775
DdeI	CTNAG	3 cut(s)	105, 146, 220
DpnI	GATC	3 cut(s)	499, 706, 791
DpnII	GATC	3 cut(s)	497, 704, 789
EaeI	YGGCCR	1 cut(s)	492
Eam1104I	CTCTTC	4 cut(s)	151, 157, 354, 677
EarI	CTCTTC	4 cut(s)	151, 157, 354, 677
EciI	GGCGGA	1 cut(s)	113
Eco130I	CCWWGG	2 cut(s)	151, 907
Eco24I	GRGCYC	1 cut(s)	800
Eco47I	GGWCC	1 cut(s)	715
Eco57I	CTGAAG	1 cut(s)	713
Eco88I	CYCGRG	1 cut(s)	579
EcoO109I	RGGNCCY	1 cut(s)	715
EcoRII	CCWGG	2 cut(s)	354, 935
EcoT14I	CCWWGG	2 cut(s)	151, 907
EcoT38I	GRGCYC	1 cut(s)	800
ErhI	CCWWGG	2 cut(s)	151, 907
FaeI	CATG	4 cut(s)	84, 155, 508, 513
FaiI	YATR	9 cut(s)	82, 153, 402, 506, 511, 521, 711, 836, 918
FalI	AAGNNNNNCTT	6 cut(s)	170, 202, 297, 329, 785, 817
FaqI	GGGAC	1 cut(s)	701
FatI	CATG	4 cut(s)	80, 151, 504, 509
FauI	CCCGC	1 cut(s)	384
FokI	GGATG	3 cut(s)	419, 736, 828
FriOI	GRGCYC	1 cut(s)	800
FspBI	CTAG	2 cut(s)	462, 468
GsaI	CCCAGC	2 cut(s)	391, 604
GsuI	CTGGAG	1 cut(s)	245
HaeIII	GGCC	3 cut(s)	228, 494, 935
HapII	CCGG	2 cut(s)	543, 588
Hin1I	GRCGYC	1 cut(s)	488
Hin1II	CATG	4 cut(s)	84, 155, 508, 513
HinfI	GANTC	2 cut(s)	58, 551
HpaII	CCGG	2 cut(s)	543, 588
HphI	GGTGA	4 cut(s)	240, 374, 406, 892
Hpy166II	GTNNAC	1 cut(s)	86
Hpy188I	TCNGA	6 cut(s)	57, 241, 594, 742, 754, 810
Hpy188III	TCNNGA	1 cut(s)	127
Hpy8I	GTNNAC	1 cut(s)	86
Hpy99I	CGWCG	2 cut(s)	490, 493
HpyAV	CCTTC	2 cut(s)	688, 894
HpyCH4III	ACNGT	2 cut(s)	378, 434
HpyCH4IV	ACGT	2 cut(s)	488, 859
HpyCH4V	TGCA	5 cut(s)	94, 346, 449, 474, 828
HpyF10VI	GCNNNNNNNGC	6 cut(s)	26, 471, 525, 660, 825, 840
HpyF3I	CTNAG	3 cut(s)	105, 146, 220
HpySE526I	ACGT	2 cut(s)	488, 859
Hsp92I	GRCGYC	1 cut(s)	488
Hsp92II	CATG	4 cut(s)	84, 155, 508, 513
Kzo9I	GATC	3 cut(s)	497, 704, 789
LmnI	GCTCC	1 cut(s)	803
Lsp1109I	GCAGC	8 cut(s)	29, 29, 41, 458, 616, 666, 840, 843
LweI	GCATC	4 cut(s)	447, 806, 860, 879
MaeI	CTAG	2 cut(s)	462, 468
MaeII	ACGT	2 cut(s)	488, 859
MaeIII	GTNAC	3 cut(s)	264, 728, 898
MalI	GATC	3 cut(s)	499, 706, 791
MboI	GATC	3 cut(s)	497, 704, 789
MboII	GAAGA	7 cut(s)	168, 174, 190, 208, 371, 694, 890
MflI	RGATCY	1 cut(s)	704
MhlI	GDGCHC	1 cut(s)	800
MluCI	AATT	2 cut(s)	232, 371
MseI	TTAA	2 cut(s)	188, 563
MspA1I	CMGCKG	1 cut(s)	391
MspI	CCGG	2 cut(s)	543, 588
MspR9I	CCNGG	3 cut(s)	356, 588, 937
Mva1269I	GAATGC	1 cut(s)	922
MvaI	CCWGG	2 cut(s)	356, 937
MwoI	GCNNNNNNNGC	6 cut(s)	26, 471, 525, 660, 825, 840
NciI	CCSGG	1 cut(s)	588
NcoI	CCATGG	1 cut(s)	151
NdeII	GATC	3 cut(s)	497, 704, 789
NlaIII	CATG	4 cut(s)	84, 155, 508, 513
NlaIV	GGNNCC	2 cut(s)	717, 799
NmuCI	GTSAC	2 cut(s)	728, 898
PaeR7I	CTCGAG	1 cut(s)	579
PcsI	WCGNNNNNNNCGW	1 cut(s)	497
PctI	GAATGC	1 cut(s)	922
PfeI	GAWTC	2 cut(s)	58, 551
PpuMI	RGGWCCY	1 cut(s)	715
PsiI	TTATAA	1 cut(s)	711
Psp5II	RGGWCCY	1 cut(s)	715
Psp6I	CCWGG	2 cut(s)	354, 935
PspFI	CCCAGC	2 cut(s)	387, 600
PspGI	CCWGG	2 cut(s)	354, 935
PspN4I	GGNNCC	2 cut(s)	717, 799
PspPI	GGNCC	2 cut(s)	227, 715
PspPPI	RGGWCCY	1 cut(s)	715
PspXI	VCTCGAGB	1 cut(s)	579
PsuI	RGATCY	1 cut(s)	704
RsaI	GTAC	1 cut(s)	776
RsaNI	GTAC	1 cut(s)	775
SaqAI	TTAA	2 cut(s)	188, 563
Sau3AI	GATC	3 cut(s)	497, 704, 789
Sau96I	GGNCC	2 cut(s)	227, 715
ScrFI	CCNGG	3 cut(s)	356, 588, 937
SduI	GDGCHC	1 cut(s)	800
SfaNI	GCATC	4 cut(s)	447, 806, 860, 879
Sfr274I	CTCGAG	1 cut(s)	579
SinI	GGWCC	1 cut(s)	715
SlaI	CTCGAG	1 cut(s)	579
SmlI	CTYRAG	1 cut(s)	579
SmoI	CTYRAG	1 cut(s)	579
Sse9I	AATT	2 cut(s)	232, 371
SsiI	CCGC	5 cut(s)	98, 391, 525, 534, 644
SspMI	CTAG	2 cut(s)	462, 468
StyD4I	CCNGG	3 cut(s)	354, 586, 935
StyI	CCWWGG	2 cut(s)	151, 907
TaaI	ACNGT	2 cut(s)	378, 434
TaiI	ACGT	2 cut(s)	491, 862
TaqI	TCGA	2 cut(s)	580, 887
TasI	AATT	2 cut(s)	232, 371
TauI	GCSGC	2 cut(s)	528, 536
TfiI	GAWTC	2 cut(s)	58, 551
Tru1I	TTAA	2 cut(s)	188, 563
Tru9I	TTAA	2 cut(s)	188, 563
TscAI	CASTG	3 cut(s)	120, 439, 664
TseFI	GTSAC	2 cut(s)	728, 898
TseI	GCWGC	8 cut(s)	17, 29, 42, 471, 604, 654, 828, 831
Tsp45I	GTSAC	2 cut(s)	728, 898
TspDTI	ATGAA	2 cut(s)	423, 493
TspRI	CASTG	3 cut(s)	120, 439, 664
VpaK11BI	GGWCC	1 cut(s)	715
XhoI	CTCGAG	1 cut(s)	579
XspI	CTAG	2 cut(s)	462, 468
ZraI	GACGTC	1 cut(s)	489

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.