Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011183

Physical Location & Seq

Genomic Coordinates Forward (+)

30603205 .. 30604718

Gene Structure 1514 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1226400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGAGGAGGTTGAGGCAGCTAACAGAGCAGCAGTTGGGAGCTGCCACAGAGTTCTCAATCTCTTGTGCAAACCAAAACCATGGAAAAACCTGCAGGTGGAGACTGAGGTGGCAGTGTTAAAGTTCAAGAGAGTCCTCTCTCTCCTCAGCCATGGAAGAGGAAGAGTGAGAAAGTTGAAGAAGAACCTAAACCCACCACCTTTCCCTCAAAATATCTTCTCAGATGGCCCTAATTATTCAGATATTTCACCAAAACCCCTCCAGTTACTCCCTCCTATTTTTCCTAAAACCCCCCACACACAAAAAAAATTTCTAGGAAACCCAGTTCCGGATGCTAATCTCAAGCTTCCCCTCCAAATTCCCAAAACAATAACTATGAAGCAGCACCAGGAAGAGAGAGAAAAGTTGCAGTTTCACCCCCAGCAGGTGAAATATCAGAGTGAAATGGTGTTTTCTGGGATAGACAGTGGCATCAACCTTGCATTTGATAGGTCTAGCTCTAGCTTCAACCCCTCCATGACATCAGCCAGATCGTTCATGTCATGTTTGAGCGTAGGCGACGGCGATGAGGTCAACACCGGCAGAGATTCTTTTCGGTTGATCGATGGCGTGCCTTGGGTTTCCGGTCAGAGTTCGCAGCAGCAAAGGAGGTGCACTGGTGTGGTGGAAAATGGGAGTGTGAATTGCGGCGGCAGTGGTAAATGCCACTATCCCAAGAAGAGGAAACTGAGGGTGAAAAGAACTTTTAAGGTCCCTGCCATTAGTGACAAGACTTCAGACATTCCTCCTGATGAGTTTTCGTGGCGGAAATACGGGCAGAAGCCGATCAAGGGCTCCCCGTACCCAAGGGGATACTACAAATGTAGCAGCATAAGAGGGTGTCCTGCGAGGAAACATGTAGAGAGATGCTTTGAAGACCCTGCTATGCTCATAGTCACCTACGAAGGCGAGCATAAGCATTCCCAATCCGCCCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Plant_zn_clust WRKY

Protein Analysis

326

Amino Acids

36.59

Weight (kDa)

9.77

Isoelectric Point (pI)

51.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Plant_zn_clust	PF10533	216 - 260	1.3e-13	Plant zinc cluster domain
WRKY	PF03106	264 - 320	3.8e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011183)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04670 AT3G04670 AT5G28650
fragaria_vesca	FvH4_6g02660 FvH4_6g02660
malus_domestica	MD04G1226400.v1.1 MD12G1243400.v1.1
prunus_persica	Prupe.6G345100_v2.0.a1
pyrus_communis	pycom04g19960 pycom12g22250
rosa_chinensis	RchiOBHm_Chr3g0450591
rosa_laevigata	RLG00000025718
rosa_multiflora	Rmu_sc0000078.1_g000042
rosa_roxburghii	Rroxscaffold_6G00428290
rosa_rugosa	Rorug02G0629000
rosa_samantha	Rh3AG029700 Rh3BG030100 Rh3CG029000 Rh3DG029800
rosa_wichuraiana	Rw3G002290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	85, 415
Acc36I	ACCTGC	3 cut(s)	85, 99, 415
AccIII	TCCGGA	1 cut(s)	328
AciI	CCGC	5 cut(s)	687, 690, 805, 969, 973
AcsI	RAATTY	2 cut(s)	308, 357
AcuI	CTGAAG	1 cut(s)	759
AfaI	GTAC	1 cut(s)	842
AfiI	CCNNNNNNNGG	5 cut(s)	97, 328, 424, 720, 830
AflIII	ACRYGT	1 cut(s)	895
AgsI	TTSAA	4 cut(s)	127, 178, 508, 914
AjnI	CCWGG	1 cut(s)	387
AleI	CACNNNNGTG	1 cut(s)	659
AloI	GAACNNNNNNTCC	2 cut(s)	309, 341
AluBI	AGCT	5 cut(s)	20, 42, 346, 498, 504
AluI	AGCT	5 cut(s)	20, 42, 346, 498, 504
Alw21I	GWGCWC	1 cut(s)	656
Alw26I	GTCTC	1 cut(s)	95
Alw44I	GTGCAC	1 cut(s)	652
Aor13HI	TCCGGA	1 cut(s)	328
AoxI	GGCC	1 cut(s)	226
ApaLI	GTGCAC	1 cut(s)	652
ApeKI	GCWGC	7 cut(s)	17, 29, 42, 382, 637, 640, 867
ApoI	RAATTY	2 cut(s)	308, 357
AspS9I	GGNCC	2 cut(s)	227, 751
AsuHPI	GGTGA	5 cut(s)	240, 407, 439, 745, 928
AvaII	GGWCC	1 cut(s)	751
BaeGI	GKGCMC	1 cut(s)	656
BanII	GRGCYC	1 cut(s)	836
BbsI	GAAGAC	1 cut(s)	921
Bbv12I	GWGCWC	1 cut(s)	656
BbvCI	CCTCAGC	1 cut(s)	146
BbvI	GCAGC	7 cut(s)	29, 29, 41, 394, 649, 652, 879
BccI	CCATC	2 cut(s)	218, 599
BceAI	ACGGC	1 cut(s)	577
BciT130I	CCWGG	1 cut(s)	389
BciVI	GTATCC	1 cut(s)	845
BcoDI	GTCTC	1 cut(s)	95
BfaI	CTAG	3 cut(s)	314, 495, 501
BfmI	CTRYAG	1 cut(s)	92
BfuAI	ACCTGC	3 cut(s)	85, 99, 415
BfuI	GTATCC	1 cut(s)	845
BisI	GCNGC	9 cut(s)	18, 30, 43, 383, 638, 641, 688, 691, 868
BlsI	GCNGC	9 cut(s)	19, 31, 44, 384, 639, 642, 689, 692, 869
Bme1390I	CCNGG	1 cut(s)	389
Bme18I	GGWCC	1 cut(s)	751
BmgT120I	GGNCC	2 cut(s)	227, 751
BmiI	GGNNCC	2 cut(s)	753, 835
BmrFI	CCNGG	1 cut(s)	389
BmrI	ACTGGG	1 cut(s)	317
BmsI	GCATC	3 cut(s)	322, 480, 896
BmuI	ACTGGG	1 cut(s)	317
BpiI	GAAGAC	1 cut(s)	921
BpmI	CTGGAG	1 cut(s)	245
Bpu10I	CCTNAGC	1 cut(s)	146
BpuEI	CTTGAG	1 cut(s)	326
Bsa29I	ATCGAT	1 cut(s)	603
BsaBI	GATNNNNATC	1 cut(s)	336
BsaJI	CCNNGG	4 cut(s)	80, 151, 614, 845
BsaWI	WCCGGW	2 cut(s)	328, 623
Bsc4I	CCNNNNNNNGG	5 cut(s)	97, 328, 424, 720, 830
Bse118I	RCCGGY	1 cut(s)	578
Bse1I	ACTGG	3 cut(s)	262, 323, 661
Bse8I	GATNNNNATC	1 cut(s)	336
BseAI	TCCGGA	1 cut(s)	328
BseBI	CCWGG	1 cut(s)	389
BseCI	ATCGAT	1 cut(s)	603
BseDI	CCNNGG	4 cut(s)	80, 151, 614, 845
BseGI	GGATG	1 cut(s)	337
BseJI	GATNNNNATC	1 cut(s)	336
BseLI	CCNNNNNNNGG	5 cut(s)	97, 328, 424, 720, 830
BseMII	CTCAG	4 cut(s)	96, 160, 234, 719
BseNI	ACTGG	3 cut(s)	262, 323, 661
BseRI	GAGGAG	2 cut(s)	20, 134
BseSI	GKGCMC	1 cut(s)	656
BseXI	GCAGC	7 cut(s)	29, 29, 41, 394, 649, 652, 879
BseYI	CCCAGC	1 cut(s)	420
BshFI	GGCC	1 cut(s)	228
BshVI	ATCGAT	1 cut(s)	603
BsiHKAI	GWGCWC	1 cut(s)	656
BsiSI	CCGG	3 cut(s)	329, 579, 624
BslFI	GGGAC	1 cut(s)	737
BslI	CCNNNNNNNGG	5 cut(s)	97, 328, 424, 720, 830
BsmAI	GTCTC	1 cut(s)	95
BsmFI	GGGAC	1 cut(s)	737
BsmI	GAATGC	1 cut(s)	958
BsnI	GGCC	1 cut(s)	228
Bsp1286I	GDGCHC	2 cut(s)	656, 836
Bsp13I	TCCGGA	1 cut(s)	328
Bsp143I	GATC	3 cut(s)	530, 600, 825
Bsp19I	CCATGG	2 cut(s)	80, 151
BspACI	CCGC	5 cut(s)	687, 690, 805, 969, 973
BspANI	GGCC	1 cut(s)	228
BspCNI	CTCAG	4 cut(s)	97, 159, 233, 720
BspDI	ATCGAT	1 cut(s)	603
BspEI	TCCGGA	1 cut(s)	328
BspLI	GGNNCC	2 cut(s)	753, 835
BspMAI	CTGCAG	1 cut(s)	96
BspMI	ACCTGC	3 cut(s)	85, 99, 415
BsrFI	RCCGGY	1 cut(s)	578
BsrI	ACTGG	3 cut(s)	262, 323, 661
BssAI	RCCGGY	1 cut(s)	578
BssECI	CCNNGG	4 cut(s)	80, 151, 614, 845
BssMI	GATC	3 cut(s)	530, 600, 825
BssT1I	CCWWGG	4 cut(s)	80, 151, 614, 845
Bst2UI	CCWGG	1 cut(s)	389
Bst4CI	ACNGT	1 cut(s)	467
Bst6I	CTCTTC	4 cut(s)	151, 157, 387, 713
BstC8I	GCNNGC	3 cut(s)	611, 950, 973
BstDEI	CTNAG	4 cut(s)	105, 146, 220, 728
BstDSI	CCRYGG	2 cut(s)	80, 151
BstF5I	GGATG	1 cut(s)	337
BstKTI	GATC	3 cut(s)	533, 603, 828
BstMAI	GTCTC	1 cut(s)	95
BstMBI	GATC	3 cut(s)	530, 600, 825
BstMWI	GCNNNNNNNGC	1 cut(s)	26
BstNI	CCWGG	1 cut(s)	389
BstNSI	RCATGY	1 cut(s)	899
BstSCI	CCNGG	1 cut(s)	387
BstSFI	CTRYAG	1 cut(s)	92
BstSLI	GKGCMC	1 cut(s)	656
BstV1I	GCAGC	7 cut(s)	29, 29, 41, 394, 649, 652, 879
BstV2I	GAAGAC	1 cut(s)	921
BstXI	CCANNNNNNTGG	1 cut(s)	81
Bsu15I	ATCGAT	1 cut(s)	603
BsuI	GTATCC	1 cut(s)	845
BsuRI	GGCC	1 cut(s)	228
BsuTUI	ATCGAT	1 cut(s)	603
BtgI	CCRYGG	2 cut(s)	80, 151
BtgZI	GCGATG	1 cut(s)	579
BtsCI	GGATG	1 cut(s)	337
BtsI	GCAGTG	2 cut(s)	120, 700
BtsIMutI	CAGTG	4 cut(s)	120, 472, 654, 700
BveI	ACCTGC	3 cut(s)	85, 99, 415
Cac8I	GCNNGC	3 cut(s)	611, 950, 973
Cfr10I	RCCGGY	1 cut(s)	578
Cfr13I	GGNCC	2 cut(s)	227, 751
ClaI	ATCGAT	1 cut(s)	603
Csp6I	GTAC	1 cut(s)	841
CviAII	CATG	6 cut(s)	81, 152, 517, 538, 543, 896
CviQI	GTAC	1 cut(s)	841
DdeI	CTNAG	4 cut(s)	105, 146, 220, 728
DpnI	GATC	3 cut(s)	532, 602, 827
DpnII	GATC	3 cut(s)	530, 600, 825
Eam1104I	CTCTTC	4 cut(s)	151, 157, 387, 713
EarI	CTCTTC	4 cut(s)	151, 157, 387, 713
EciI	GGCGGA	2 cut(s)	820, 958
Eco130I	CCWWGG	4 cut(s)	80, 151, 614, 845
Eco24I	GRGCYC	1 cut(s)	836
Eco47I	GGWCC	1 cut(s)	751
Eco57I	CTGAAG	1 cut(s)	759
EcoO109I	RGGNCCY	1 cut(s)	751
EcoRII	CCWGG	1 cut(s)	387
EcoT14I	CCWWGG	4 cut(s)	80, 151, 614, 845
EcoT38I	GRGCYC	1 cut(s)	836
ErhI	CCWWGG	4 cut(s)	80, 151, 614, 845
FaeI	CATG	6 cut(s)	84, 155, 520, 541, 546, 899
FaqI	GGGAC	1 cut(s)	737
FatI	CATG	6 cut(s)	80, 151, 516, 537, 542, 895
Fnu4HI	GCNGC	9 cut(s)	18, 30, 43, 383, 638, 641, 688, 691, 868
FokI	GGATG	1 cut(s)	344
FriOI	GRGCYC	1 cut(s)	836
Fsp4HI	GCNGC	9 cut(s)	18, 30, 43, 383, 638, 641, 688, 691, 868
FspBI	CTAG	3 cut(s)	314, 495, 501
GluI	GCNGC	9 cut(s)	18, 30, 43, 383, 638, 641, 688, 691, 868
GsaI	CCCAGC	1 cut(s)	424
GsuI	CTGGAG	1 cut(s)	245
HaeIII	GGCC	1 cut(s)	228
HapII	CCGG	3 cut(s)	329, 579, 624
Hin1II	CATG	6 cut(s)	84, 155, 520, 541, 546, 899
HincII	GTYRAC	1 cut(s)	574
HindII	GTYRAC	1 cut(s)	574
HindIII	AAGCTT	1 cut(s)	344
HinfI	GANTC	2 cut(s)	132, 587
HpaII	CCGG	3 cut(s)	329, 579, 624
HphI	GGTGA	5 cut(s)	240, 407, 439, 745, 928
Hpy166II	GTNNAC	2 cut(s)	574, 654
Hpy188I	TCNGA	5 cut(s)	223, 241, 438, 630, 778
Hpy188III	TCNNGA	3 cut(s)	127, 329, 788
Hpy8I	GTNNAC	2 cut(s)	574, 654
Hpy99I	CGWCG	1 cut(s)	563
HpyAV	CCTTC	1 cut(s)	938
HpyCH4III	ACNGT	1 cut(s)	467
HpyCH4V	TGCA	5 cut(s)	69, 94, 409, 482, 654
HpyF10VI	GCNNNNNNNGC	1 cut(s)	26
HpyF3I	CTNAG	4 cut(s)	105, 146, 220, 728
Hsp92II	CATG	6 cut(s)	84, 155, 520, 541, 546, 899
Kpn2I	TCCGGA	1 cut(s)	328
Kzo9I	GATC	3 cut(s)	530, 600, 825
LmnI	GCTCC	2 cut(s)	39, 839
Lsp1109I	GCAGC	7 cut(s)	29, 29, 41, 394, 649, 652, 879
LweI	GCATC	3 cut(s)	322, 480, 896
MaeI	CTAG	3 cut(s)	314, 495, 501
MaeIII	GTNAC	3 cut(s)	264, 764, 934
MalI	GATC	3 cut(s)	532, 602, 827
MboI	GATC	3 cut(s)	530, 600, 825
MboII	GAAGA	8 cut(s)	168, 174, 190, 193, 208, 404, 730, 926
MhlI	GDGCHC	2 cut(s)	656, 836
MluCI	AATT	4 cut(s)	232, 308, 357, 682
MlyI	GAGTC	1 cut(s)	141
MroI	TCCGGA	1 cut(s)	328
MseI	TTAA	2 cut(s)	119, 747
MslI	CAYNNNNRTG	1 cut(s)	659
MspA1I	CMGCKG	1 cut(s)	975
MspI	CCGG	3 cut(s)	329, 579, 624
MspR9I	CCNGG	1 cut(s)	389
Mva1269I	GAATGC	1 cut(s)	958
MvaI	CCWGG	1 cut(s)	389
MwoI	GCNNNNNNNGC	1 cut(s)	26
NcoI	CCATGG	2 cut(s)	80, 151
NdeII	GATC	3 cut(s)	530, 600, 825
NlaIII	CATG	6 cut(s)	84, 155, 520, 541, 546, 899
NlaIV	GGNNCC	2 cut(s)	753, 835
NmuCI	GTSAC	2 cut(s)	764, 934
NspI	RCATGY	1 cut(s)	899
OliI	CACNNNNGTG	1 cut(s)	659
PaqCI	CACCTGC	2 cut(s)	85, 415
PciI	ACATGT	1 cut(s)	895
PctI	GAATGC	1 cut(s)	958
PfeI	GAWTC	1 cut(s)	587
PkrI	GCNGC	9 cut(s)	19, 31, 44, 384, 639, 642, 689, 692, 869
PleI	GAGTC	1 cut(s)	140
PpsI	GAGTC	1 cut(s)	140
PpuMI	RGGWCCY	1 cut(s)	751
PscI	ACATGT	1 cut(s)	895
Psp5II	RGGWCCY	1 cut(s)	751
Psp6I	CCWGG	1 cut(s)	387
PspFI	CCCAGC	1 cut(s)	420
PspGI	CCWGG	1 cut(s)	387
PspN4I	GGNNCC	2 cut(s)	753, 835
PspPI	GGNCC	2 cut(s)	227, 751
PspPPI	RGGWCCY	1 cut(s)	751
PstI	CTGCAG	1 cut(s)	96
RsaI	GTAC	1 cut(s)	842
RsaNI	GTAC	1 cut(s)	841
RseI	CAYNNNNRTG	1 cut(s)	659
SaqAI	TTAA	2 cut(s)	119, 747
SatI	GCNGC	9 cut(s)	18, 30, 43, 383, 638, 641, 688, 691, 868
Sau3AI	GATC	3 cut(s)	530, 600, 825
Sau96I	GGNCC	2 cut(s)	227, 751
SbfI	CCTGCAGG	1 cut(s)	96
SchI	GAGTC	1 cut(s)	141
ScrFI	CCNGG	1 cut(s)	389
SdaI	CCTGCAGG	1 cut(s)	96
SduI	GDGCHC	2 cut(s)	656, 836
SfaNI	GCATC	3 cut(s)	322, 480, 896
SfcI	CTRYAG	1 cut(s)	92
SinI	GGWCC	1 cut(s)	751
SmiMI	CAYNNNNRTG	1 cut(s)	659
SmlI	CTYRAG	1 cut(s)	341
SmoI	CTYRAG	1 cut(s)	341
Sse8387I	CCTGCAGG	1 cut(s)	96
Sse9I	AATT	4 cut(s)	232, 308, 357, 682
SsiI	CCGC	5 cut(s)	687, 690, 805, 969, 973
SspMI	CTAG	3 cut(s)	314, 495, 501
StyD4I	CCNGG	1 cut(s)	387
StyI	CCWWGG	4 cut(s)	80, 151, 614, 845
TaaI	ACNGT	1 cut(s)	467
TaqI	TCGA	1 cut(s)	603
TasI	AATT	4 cut(s)	232, 308, 357, 682
TauI	GCSGC	2 cut(s)	690, 693
TfiI	GAWTC	1 cut(s)	587
Tru1I	TTAA	2 cut(s)	119, 747
Tru9I	TTAA	2 cut(s)	119, 747
TscAI	CASTG	4 cut(s)	120, 472, 661, 700
TseFI	GTSAC	2 cut(s)	764, 934
TseI	GCWGC	7 cut(s)	17, 29, 42, 382, 637, 640, 867
Tsp45I	GTSAC	2 cut(s)	764, 934
TspDTI	ATGAA	2 cut(s)	392, 526
TspRI	CASTG	4 cut(s)	120, 472, 661, 700
VneI	GTGCAC	1 cut(s)	652
VpaK11BI	GGWCC	1 cut(s)	751
XapI	RAATTY	2 cut(s)	308, 357
XceI	RCATGY	1 cut(s)	899
XspI	CTAG	3 cut(s)	314, 495, 501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.