FvH4_6g06170
MYB Family

Protein ALWAYS EARLY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002363
Physical Location & Seq
Forward (+)
3516174 .. 3527560
11387 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06170.t12

Sequence Viewer

Length: 3471 bp
ATGGCGCCCACCAGAAAAGCTAAAACCGTGAAACGGTATTCAAATTTGAGTGAGGCCTCTCCTGGAGGCAAAAATAAGCAAGGGAAGAGAAAGTTTACTGATCAGTTAGGACCGCAGTGGAGCAAGGGAGAGCTTCAGCGGTTCTATGAAGCCTACCGGAAATATGGGCAGAATTGGAAGAAGGTGGCTGCTGTAGTGCGCAACAGAAATATTGAGATGGTGGAGGCTCTTTACAATATGAACCGAGCGTACTTGTCATTGCCAGAGGGCACAGCTTCTGTTGTTGGCCTTATAGCAATGATGACAGATCACTATAATGTTATGGAAGGCAGTGAAAGTGAACGGGAAAGCAATGATGCTATAAGATATTCTCGGAAACCCCAGAAACGTAAACTTGGTAAAGATAGTGTGTCGAAAGACATGTTCCAGCCTCATTCTACTGCTTCAGTAGATGGATGCCTATCACACTTAAAACGAAGAAGACTTGATGGTAACCAGCCTCGTGCAGTTGGGAAGAGAACACCACGCTTCCCAGTTCAATATTCATCTAAGAAAGATGATGGAGAAAATTATGCTTCTCCAATTAGAAAGGGCCGGAAATCAGAGGCAGATAATGAGTATGATGTCGCCAAGGTTGCAGCATTATTAACTGAGGCTTCTCACAGAGGAGGCTCTCCCCGACTTTCGCAAACACCATATAGAAGATTTACTGTTCAGAGCTCGCAAAGAATGCAGCCACCATCATGGAAAGCTCGAGCCGACCTTCGTGATGCTTCAATGGAAGAAGACTGTCTGGAAGGTAGCGTAGGAAGTAGAGGGGCTGAAACTGGAGATTATACCAGAGATAGTAGCTCCTTGATGGACATGGAAGGTGTAGGTACAGTAGAAATTAGGAGGGGAAAGAAAGTTTATGGAAAGAAAGAGAAAGTTAAAGACATTGGAAACCATCAATTTGATGATGGTGGTGAAGCATGTAGTGGCACTGAAGAAGGACTTCATGTCAGTACCAAGGGAAAAACTGATATTGAAGTTTCAGATGCTAAAGGAGATCAGTTTTACTCACAAGTTCCAAGGCAGGAATGCGGGAAACCATATTTTGGAGATGATAGTGCAGAACTGGATGCTCTGGAAGCTTTGCACACTTTGGCTGATTTGTCATTGATGATGCCAGCGTCCACAATGGAATCTGGGTCCTCAATGCAGTTGAAGGACGAAAGAACAGCTTCCGAGATGGAAGAAAAATCTAATATGCCTGAATCCACATCTACCAGCCAATTTAGAAACAAGAATAAGCTCCCAGGGGGGAAACAGAGAGAACCCATTGCAGATTCTCGAACTGAGGGTACTAATGCCAAAAAATCCAAACTTGCAAGGGACTCGACACCCATTGATATCAATGTTGTCTCCGAATCAGAACAGCTCCACTCTACCAGCAAATCATGGAGAAGGAAGCGGAAACCCACAGTATCAAAGATATCAAATGCCGAAGCAAATATTGATTCTACTGTGAATGAACCTTCATATTCTGAGGTCTTTGGTCAAGAAGAGAATAAATCAGTGGTTAAAGGGAGACGTACTGGTCAAATCTCCACTCCTTCAAAACAATGGAAGTCGGTCAGACCAGGGGACGGCTCTTTGAATAGTGATTTTGGGCAAACAGTGAGTAATGTAATGGTAGCAACTGCAGAAGGTCGACCCACTGCAAAGCAAGTTCATTCCCCAACTAAACAAAGTAGACGTAAACGGTATCTACCCAGAGCAACGGTGCCCAAGTCATCTGAGAACATATTAAAAACTCAACTTCATAAACACTCCAACTCACTGCAGGACAGAATTCTCTATCTAAAGGAGAAGATGTCTTGTTGCTTGTCATCACATCTGGTACGTAGATGGTGCACGTTTGAGTGGTTTTATAGTGCAATTGATTACCCTTGGTTTGCCAAAAGAGAGTTTGAGGAGTACTTAAATCATGTTGGACTTGGACACATTCCGAGGCTAACTCGTGTTGAATGGGGTGTGATTAGAAGTTCCCTGGGAAAACCTCGGAGGTTTTCTGAGCACTTCCTATCTGAAGAAAGAGAGAAACTTAAACAATATCGGGAATCTGTGAGAAAACATTATGCTGAACTCCGCACTGGTGTTAGGGAGGGACTTCCAACAGATTTGGCAAGACCTTTATCAGTTGGACAACGTGTCATCGCTCTTCATCCCAAAACAAGAGAAGTTCACGATGGAAGTGTGCTCACTGTTGATCACGATAAGTGCAGGGTTCAGTTTGATCGTCCAGAGATAGGTGTTGAATTTGTCATGGATGTGGATTGTATGCCTTCAAACCCATTGGATAACATGCCTGAAGCCCTTAAGAGACAGAATATTGCTTTTGACAAATTCCCTCTCACATTACCCCATATGAATGGGAATTTAAATTTTGAAAGACCTGTGATGAATGTTTCAAGTGGGCTTTTGGAGAAAGATCCCAGCCCCATGAATACTTCCTTACCGCAGGGAAAGGGAGATACAAACCATATAATTTCACAACCAAAAGGAGCATCTGTTGATATTGCTGGTGCACAGACAGCGGTTAGTCAACCTGGCGTGGTGGCACACAATCAAGCAAGGGAAGCTGATATACAAGCTCTTTCAGACCTGAATCGTGCTCTTGACAAAAAGAAAGCATTGTTGATGGAGCTTAGCACAATTAATAACATCCTGGAAAACCAGAACAGTGGAGAATGCTCTTTAAAAGATTGCGATAAGAAGCATTACGCCACAGTACTTGTACAGCTAAAGGAAGCCAGTGGCCAGGAATCTTCTGCTTTGCTTAATTTGAGGCACCGAAATACTTACCCTGGAAATACCCTGCCTCCTTCGCTGAAATACCCAACGAATTCCACAGTCTATGGGAGCCTCCCTAGTTCCTTTGATATATCTACTTCTCAAGAGTCAGGATCCAGCGTTGCTGAAATTGTAGAAGTTTCAAGATTGAAAGCACATACTTTGGTAAATGCTGCTGTTCAGGCAATGTCATCAAGGAAGGAAGGGGAAGAAGCTTATGTAAGGGTAAGGAGAGCTCTTGATTCCATCAATATTCAGAATCTGACATCAGACTCTAGGTCAGCAGTCAACAGGACTCAAGAGCAGGTCAATGGCACATTGGGACATCGCAACCAGTTGACTTCCGTTGCATCAGAGCCCAACACTAGTGATTCATCTGGTCCCAAGTTACATACTGAGACGGATAAAAATGAATCACAGATGCTTTCGGAGGTTATCACTGCTTGCGTCATGGCTATGAACATGATACAGACATGTACTGAACGACAATATCCTCCGGCTGTTGTGGCTCAAGTATTAGATTATGCAGTCACAAGCTTGCATCCACGTTGTCCCCAAAATGTTGGAATGTACAGAGAAATTCAAATGTGCATGGGCAGAATCAAGACCCAGATATTAGCACTCGTGCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
DIRP Myb_DNA-binding

Protein Analysis

1157

Amino Acids

128.98

Weight (kDa)

9.2

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIRP PF06584 495 - 596 1.1e-34 DIRP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1578
Acc16I TGCGCA 1 cut(s) 200
Acc36I ACCTGC 1 cut(s) 3130
AccB1I GGYRCC 3 cut(s) 4, 1765, 2832
AccB7I CCANNNNNTGG 1 cut(s) 1097
AccI GTMKAC 2 cut(s) 1693, 1735
AciI CCGC 7 cut(s) 113, 139, 1083, 1453, 2131, 2501, 2579
AclWI GGATC 3 cut(s) 2468, 2943, 2956
AcoI YGGCCR 1 cut(s) 2800
AcsI RAATTY 8 cut(s) 43, 1833, 2300, 2387, 2419, 2425, 2887, 3414
AcuI CTGAAG 5 cut(s) 119, 429, 1005, 2091, 2373
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 4 cut(s) 33, 1097, 1628, 2291
AflII CTTAAG 1 cut(s) 2360
AflIII ACRYGT 3 cut(s) 420, 2191, 3308
AhdI GACNNNNNGTC 3 cut(s) 998, 2192, 3112
AhlI ACTAGT 1 cut(s) 3200
AjnI CCWGG 8 cut(s) 61, 1297, 1621, 2031, 2590, 2709, 2802, 2848
Alw21I GWGCWC 7 cut(s) 722, 1898, 2061, 2244, 2572, 2659, 3073
Alw26I GTCTC 4 cut(s) 1408, 1564, 2359, 3227
Alw44I GTGCAC 2 cut(s) 1894, 2568
AlwI GGATC 3 cut(s) 2468, 2943, 2956
AlwNI CAGNNNCTG 2 cut(s) 278, 3097
Ama87I CYCGRG 1 cut(s) 753
AoxI GGCC 4 cut(s) 54, 286, 592, 2800
ApaLI GTGCAC 2 cut(s) 1894, 2568
ApeKI GCWGC 4 cut(s) 188, 638, 733, 3008
ApoI RAATTY 8 cut(s) 43, 1833, 2300, 2387, 2419, 2425, 2887, 3414
AseI ATTAAT 1 cut(s) 2700
Asp700I GAANNNNTTC 2 cut(s) 993, 1222
AspLEI GCGC 2 cut(s) 7, 201
AspS9I GGNCC 4 cut(s) 110, 592, 1191, 3215
AsuHPI GGTGA 1 cut(s) 977
AvaI CYCGRG 1 cut(s) 753
AvaII GGWCC 3 cut(s) 110, 1191, 3215
BaeGI GKGCMC 4 cut(s) 272, 1770, 1898, 2572
BalI TGGCCA 1 cut(s) 2802
BamHI GGATCC 1 cut(s) 2948
BanI GGYRCC 3 cut(s) 4, 1765, 2832
BanII GRGCYC 3 cut(s) 722, 3073, 3195
BarI GAAGNNNNNNTAC 2 cut(s) 2613, 2645
BauI CACGAG 3 cut(s) 501, 2001, 3458
BbsI GAAGAC 2 cut(s) 487, 792
Bbv12I GWGCWC 7 cut(s) 722, 1898, 2061, 2244, 2572, 2659, 3073
BbvI GCAGC 4 cut(s) 175, 650, 745, 2995
BceAI ACGGC 1 cut(s) 1645
BcgI CGANNNNNNTGC 2 cut(s) 1359, 1393
BciT130I CCWGG 8 cut(s) 63, 1299, 1623, 2033, 2592, 2711, 2804, 2850
BclI TGATCA 2 cut(s) 100, 2251
BcoDI GTCTC 4 cut(s) 1408, 1564, 2359, 3227
BcuI ACTAGT 1 cut(s) 3200
BfaI CTAG 3 cut(s) 2913, 3111, 3201
BfmI CTRYAG 3 cut(s) 192, 1683, 1823
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 2360
BfuAI ACCTGC 1 cut(s) 3130
BisI GCNGC 4 cut(s) 189, 639, 734, 3009
BlpI GCTNAGC 1 cut(s) 2690
BlsI GCNGC 4 cut(s) 190, 640, 735, 3010
BmcAI AGTACT 2 cut(s) 1961, 2775
Bme1390I CCNGG 8 cut(s) 63, 1299, 1623, 2033, 2592, 2711, 2804, 2850
Bme18I GGWCC 3 cut(s) 110, 1191, 3215
BmeRI GACNNNNNGTC 3 cut(s) 998, 2192, 3112
BmeT110I CYCGRG 1 cut(s) 753
BmgT120I GGNCC 4 cut(s) 110, 592, 1191, 3215
BmiI GGNNCC 7 cut(s) 6, 1192, 1767, 2834, 2906, 2950, 3217
BmrFI CCNGG 8 cut(s) 63, 1299, 1623, 2033, 2592, 2711, 2804, 2850
BmrI ACTGGG 1 cut(s) 527
BmuI ACTGGG 1 cut(s) 527
BpiI GAAGAC 2 cut(s) 487, 792
BplI GAGNNNNNCTC 2 cut(s) 1984, 2016
BpmI CTGGAG 2 cut(s) 84, 849
Bpu1102I GCTNAGC 1 cut(s) 2690
BpuEI CTTGAG 3 cut(s) 2922, 3117, 3330
BsaAI YACGTR 1 cut(s) 1886
BsaBI GATNNNNATC 1 cut(s) 460
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 156
BsaXI ACNNNNNCTCC 6 cut(s) 1841, 1871, 2538, 2568, 2848, 2878
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 1097, 1628, 2291
Bse1I ACTGG 7 cut(s) 533, 832, 1122, 1582, 2140, 2796, 3169
Bse3DI GCAATG 5 cut(s) 257, 303, 358, 1320, 3027
Bse8I GATNNNNATC 1 cut(s) 460
BseBI CCWGG 8 cut(s) 63, 1299, 1623, 2033, 2592, 2711, 2804, 2850
BseGI GGATG 6 cut(s) 461, 1126, 2206, 2317, 2706, 3376
BseJI GATNNNNATC 1 cut(s) 460
BseLI CCNNNNNNNGG 4 cut(s) 33, 1097, 1628, 2291
BseMI GCAATG 5 cut(s) 257, 303, 358, 1320, 3027
BseMII CTCAG 6 cut(s) 642, 1329, 1518, 1770, 2046, 3222
BseNI ACTGG 7 cut(s) 533, 832, 1122, 1582, 2140, 2796, 3169
BseRI GAGGAG 2 cut(s) 681, 1970
BseSI GKGCMC 4 cut(s) 272, 1770, 1898, 2572
BseXI GCAGC 4 cut(s) 175, 650, 745, 2995
BseYI CCCAGC 1 cut(s) 2477
BsgI GTGCAG 3 cut(s) 525, 1131, 2284
BshFI GGCC 4 cut(s) 56, 288, 594, 2802
BshNI GGYRCC 3 cut(s) 4, 1765, 2832
BsiHKAI GWGCWC 7 cut(s) 722, 1898, 2061, 2244, 2572, 2659, 3073
BsiHKCI CYCGRG 1 cut(s) 753
BsiSI CCGG 3 cut(s) 157, 595, 3332
BslFI GGGAC 6 cut(s) 1388, 1640, 2163, 3171, 3201, 3372
BslI CCNNNNNNNGG 4 cut(s) 33, 1097, 1628, 2291
BsmAI GTCTC 4 cut(s) 1408, 1564, 2359, 3227
BsmBI CGTCTC 2 cut(s) 1564, 3227
BsmFI GGGAC 6 cut(s) 1388, 1640, 2163, 3171, 3201, 3372
BsmI GAATGC 3 cut(s) 735, 1085, 2738
BsnI GGCC 4 cut(s) 56, 288, 594, 2802
BsoBI CYCGRG 1 cut(s) 753
Bsp1407I TGTACA 2 cut(s) 2779, 3405
Bsp143I GATC 7 cut(s) 100, 307, 1048, 2251, 2278, 2473, 2948
Bsp1720I GCTNAGC 1 cut(s) 2690
BspACI CCGC 7 cut(s) 113, 139, 1083, 1453, 2131, 2501, 2579
BspANI GGCC 4 cut(s) 56, 288, 594, 2802
BspCNI CTCAG 6 cut(s) 643, 1330, 1519, 1771, 2047, 3223
BspLI GGNNCC 7 cut(s) 6, 1192, 1767, 2834, 2906, 2950, 3217
BspMAI CTGCAG 2 cut(s) 1687, 1827
BspMI ACCTGC 1 cut(s) 3130
BspPI GGATC 3 cut(s) 2468, 2943, 2956
BspQI GCTCTTC 1 cut(s) 2208
BspT107I GGYRCC 3 cut(s) 4, 1765, 2832
BspTI CTTAAG 1 cut(s) 2360
BsrDI GCAATG 5 cut(s) 257, 303, 358, 1320, 3027
BsrGI TGTACA 2 cut(s) 2779, 3405
BsrI ACTGG 7 cut(s) 533, 832, 1122, 1582, 2140, 2796, 3169
BssMI GATC 7 cut(s) 100, 307, 1048, 2251, 2278, 2473, 2948
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 3 cut(s) 501, 2001, 3458
BssT1I CCWWGG 4 cut(s) 630, 1008, 1070, 1931
Bst2BI CACGAG 3 cut(s) 501, 2001, 3458
Bst2UI CCWGG 8 cut(s) 63, 1299, 1623, 2033, 2592, 2711, 2804, 2850
Bst6I CTCTTC 4 cut(s) 80, 509, 1539, 2208
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 2360
BstAPI GCANNNNNTGC 1 cut(s) 730
BstAUI TGTACA 2 cut(s) 2779, 3405
BstBAI YACGTR 1 cut(s) 1886
BstC8I GCNNGC 4 cut(s) 722, 1170, 3280, 3374
BstDEI CTNAG 8 cut(s) 549, 651, 1338, 1527, 1779, 2055, 2690, 3231
BstEII GGTNACC 1 cut(s) 491
BstF5I GGATG 6 cut(s) 461, 1126, 2206, 2317, 2706, 3376
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 201
BstKTI GATC 7 cut(s) 103, 310, 1051, 2254, 2281, 2476, 2951
BstMAI GTCTC 4 cut(s) 1408, 1564, 2359, 3227
BstMBI GATC 7 cut(s) 100, 307, 1048, 2251, 2278, 2473, 2948
BstMWI GCNNNNNNNGC 8 cut(s) 635, 730, 1130, 2576, 2621, 2869, 3017, 3341
BstNI CCWGG 8 cut(s) 63, 1299, 1623, 2033, 2592, 2711, 2804, 2850
BstNSI RCATGY 4 cut(s) 424, 975, 2350, 3312
BstPI GGTNACC 1 cut(s) 491
BstSCI CCNGG 8 cut(s) 61, 1297, 1621, 2031, 2590, 2709, 2802, 2848
BstSFI CTRYAG 3 cut(s) 192, 1683, 1823
BstSLI GKGCMC 4 cut(s) 272, 1770, 1898, 2572
BstSNI TACGTA 1 cut(s) 1886
BstV1I GCAGC 4 cut(s) 175, 650, 745, 2995
BstV2I GAAGAC 2 cut(s) 487, 792
BstX2I RGATCY 2 cut(s) 2473, 2948
BstXI CCANNNNNNTGG 4 cut(s) 744, 2414, 2726, 3398
BstYI RGATCY 2 cut(s) 2473, 2948
BsuRI GGCC 4 cut(s) 56, 288, 594, 2802
BtgZI GCGATG 2 cut(s) 2182, 3146
BtsCI GGATG 6 cut(s) 461, 1126, 2206, 2317, 2706, 3376
BtsI GCAGTG 5 cut(s) 122, 337, 1698, 1820, 3273
BveI ACCTGC 1 cut(s) 3130
Cac8I GCNNGC 4 cut(s) 722, 1170, 3280, 3374
CaiI CAGNNNCTG 2 cut(s) 278, 3097
CfoI GCGC 2 cut(s) 7, 201
Cfr13I GGNCC 4 cut(s) 110, 592, 1191, 3215
CseI GACGC 2 cut(s) 1161, 3271
CspCI CAANNNNNGTGG 2 cut(s) 2758, 2793
DdeI CTNAG 8 cut(s) 549, 651, 1338, 1527, 1779, 2055, 2690, 3231
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 102, 309, 1050, 2253, 2280, 2475, 2950
DpnII GATC 7 cut(s) 100, 307, 1048, 2251, 2278, 2473, 2948
DraI TTTAAA 2 cut(s) 2424, 2742
DrdI GACNNNNNNGTC 1 cut(s) 1578
DriI GACNNNNNGTC 3 cut(s) 998, 2192, 3112
DseDI GACNNNNNNGTC 1 cut(s) 1578
EaeI YGGCCR 1 cut(s) 2800
Eam1104I CTCTTC 4 cut(s) 80, 509, 1539, 2208
Eam1105I GACNNNNNGTC 3 cut(s) 998, 2192, 3112
EarI CTCTTC 4 cut(s) 80, 509, 1539, 2208
Ecl136II GAGCTC 2 cut(s) 720, 3071
Eco105I TACGTA 1 cut(s) 1886
Eco130I CCWWGG 4 cut(s) 630, 1008, 1070, 1931
Eco147I AGGCCT 1 cut(s) 56
Eco24I GRGCYC 3 cut(s) 722, 3073, 3195
Eco32I GATATC 2 cut(s) 1393, 1476
Eco47I GGWCC 3 cut(s) 110, 1191, 3215
Eco53kI GAGCTC 2 cut(s) 720, 3071
Eco57I CTGAAG 5 cut(s) 119, 429, 1005, 2091, 2373
Eco88I CYCGRG 1 cut(s) 753
Eco91I GGTNACC 1 cut(s) 491
EcoICRI GAGCTC 2 cut(s) 720, 3071
EcoO109I RGGNCCY 1 cut(s) 1191
EcoO65I GGTNACC 1 cut(s) 491
EcoRI GAATTC 2 cut(s) 1833, 2887
EcoRII CCWGG 8 cut(s) 61, 1297, 1621, 2031, 2590, 2709, 2802, 2848
EcoRV GATATC 2 cut(s) 1393, 1476
EcoT14I CCWWGG 4 cut(s) 630, 1008, 1070, 1931
EcoT38I GRGCYC 3 cut(s) 722, 3073, 3195
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 630, 1008, 1070, 1931
Esp3I CGTCTC 2 cut(s) 1564, 3227
FalI AAGNNNNNCTT 4 cut(s) 978, 1010, 1207, 1239
FaqI GGGAC 6 cut(s) 1388, 1640, 2163, 3171, 3201, 3372
FauI CCCGC 1 cut(s) 1076
FauNDI CATATG 1 cut(s) 2409
FbaI TGATCA 2 cut(s) 100, 2251
FblI GTMKAC 2 cut(s) 1693, 1735
Fnu4HI GCNGC 4 cut(s) 189, 639, 734, 3009
FokI GGATG 6 cut(s) 468, 1133, 2193, 2324, 2693, 3363
FriOI GRGCYC 3 cut(s) 722, 3073, 3195
Fsp4HI GCNGC 4 cut(s) 189, 639, 734, 3009
FspBI CTAG 3 cut(s) 2913, 3111, 3201
FspI TGCGCA 1 cut(s) 200
GlaI GCGC 2 cut(s) 6, 200
GluI GCNGC 4 cut(s) 189, 639, 734, 3009
GsaI CCCAGC 1 cut(s) 2481
GsuI CTGGAG 2 cut(s) 84, 849
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 56, 288, 594, 2802
HapII CCGG 3 cut(s) 157, 595, 3332
HgaI GACGC 2 cut(s) 1161, 3271
HhaI GCGC 2 cut(s) 7, 201
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 5, 199
HinP1I GCGC 2 cut(s) 5, 199
HincII GTYRAC 4 cut(s) 1694, 2588, 3124, 3174
HindII GTYRAC 4 cut(s) 1694, 2588, 3124, 3174
HindIII AAGCTT 3 cut(s) 1131, 3048, 3370
HpaII CCGG 3 cut(s) 157, 595, 3332
HphI GGTGA 1 cut(s) 977
HpyCH4IV ACGT 7 cut(s) 388, 1573, 1738, 1885, 1898, 2191, 3382
HpyF10VI GCNNNNNNNGC 8 cut(s) 635, 730, 1130, 2576, 2621, 2869, 3017, 3341
HpyF3I CTNAG 8 cut(s) 549, 651, 1338, 1527, 1779, 2055, 2690, 3231
HpySE526I ACGT 7 cut(s) 388, 1573, 1738, 1885, 1898, 2191, 3382
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 5, 199
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 2 cut(s) 100, 2251
Kzo9I GATC 7 cut(s) 100, 307, 1048, 2251, 2278, 2473, 2948
LguI GCTCTTC 1 cut(s) 2208
LmnI GCTCC 7 cut(s) 120, 857, 1299, 1425, 2546, 2686, 2904
Lsp1109I GCAGC 4 cut(s) 175, 650, 745, 2995
MaeI CTAG 3 cut(s) 2913, 3111, 3201
MaeII ACGT 7 cut(s) 388, 1573, 1738, 1885, 1898, 2191, 3382
MaeIII GTNAC 3 cut(s) 491, 3222, 3364
MalI GATC 7 cut(s) 102, 309, 1050, 2253, 2280, 2475, 2950
MboI GATC 7 cut(s) 100, 307, 1048, 2251, 2278, 2473, 2948
MfeI CAATTG 1 cut(s) 1920
MflI RGATCY 2 cut(s) 2473, 2948
MlsI TGGCCA 1 cut(s) 2802
MluNI TGGCCA 1 cut(s) 2802
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 4 cut(s) 1370, 2951, 3101, 3124
MmeI TCCRAC 5 cut(s) 1839, 1954, 2164, 2180, 3379
Mox20I TGGCCA 1 cut(s) 2802
MroXI GAANNNNTTC 2 cut(s) 993, 1222
MscI TGGCCA 1 cut(s) 2802
MslI CAYNNNNRTG 5 cut(s) 315, 742, 2312, 2412, 3290
Msp20I TGGCCA 1 cut(s) 2802
MspA1I CMGCKG 2 cut(s) 139, 2579
MspCI CTTAAG 1 cut(s) 2360
MspI CCGG 3 cut(s) 157, 595, 3332
MspR9I CCNGG 8 cut(s) 63, 1299, 1623, 2033, 2592, 2711, 2804, 2850
MunI CAATTG 1 cut(s) 1920
Mva1269I GAATGC 3 cut(s) 735, 1085, 2738
MvaI CCWGG 8 cut(s) 63, 1299, 1623, 2033, 2592, 2711, 2804, 2850
MwoI GCNNNNNNNGC 8 cut(s) 635, 730, 1130, 2576, 2621, 2869, 3017, 3341
NarI GGCGCC 1 cut(s) 5
NdeI CATATG 1 cut(s) 2409
NdeII GATC 7 cut(s) 100, 307, 1048, 2251, 2278, 2473, 2948
NlaIV GGNNCC 7 cut(s) 6, 1192, 1767, 2834, 2906, 2950, 3217
NmuCI GTSAC 1 cut(s) 3364
NsbI TGCGCA 1 cut(s) 200
NspI RCATGY 4 cut(s) 424, 975, 2350, 3312
PaeR7I CTCGAG 1 cut(s) 753
PasI CCCWGGG 2 cut(s) 1298, 2032
PceI AGGCCT 1 cut(s) 56
PciI ACATGT 2 cut(s) 420, 3308
PciSI GCTCTTC 1 cut(s) 2208
PctI GAATGC 3 cut(s) 735, 1085, 2738
PdmI GAANNNNTTC 2 cut(s) 993, 1222
PflMI CCANNNNNTGG 1 cut(s) 1097
PfoI TCCNGGA 2 cut(s) 61, 2709
PkrI GCNGC 4 cut(s) 190, 640, 735, 3010
PleI GAGTC 4 cut(s) 1370, 2950, 3101, 3124
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 4 cut(s) 1370, 2950, 3101, 3124
Ppu21I YACGTR 1 cut(s) 1886
PpuMI RGGWCCY 1 cut(s) 1191
PscI ACATGT 2 cut(s) 420, 3308
PshBI ATTAAT 1 cut(s) 2700
Psp124BI GAGCTC 2 cut(s) 722, 3073
Psp5II RGGWCCY 1 cut(s) 1191
Psp6I CCWGG 8 cut(s) 61, 1297, 1621, 2031, 2590, 2709, 2802, 2848
PspEI GGTNACC 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 2477
PspGI CCWGG 8 cut(s) 61, 1297, 1621, 2031, 2590, 2709, 2802, 2848
PspN4I GGNNCC 7 cut(s) 6, 1192, 1767, 2834, 2906, 2950, 3217
PspPI GGNCC 4 cut(s) 110, 592, 1191, 3215
PspPPI RGGWCCY 1 cut(s) 1191
PspXI VCTCGAGB 1 cut(s) 753
PsrI GAACNNNNNNTAC 4 cut(s) 233, 265, 1327, 1359
PstI CTGCAG 2 cut(s) 1687, 1827
PstNI CAGNNNCTG 2 cut(s) 278, 3097
PsuI RGATCY 2 cut(s) 2473, 2948
RseI CAYNNNNRTG 5 cut(s) 315, 742, 2312, 2412, 3290
SacI GAGCTC 2 cut(s) 722, 3073
SalI GTCGAC 1 cut(s) 1692
SapI GCTCTTC 1 cut(s) 2208
SatI GCNGC 4 cut(s) 189, 639, 734, 3009
Sau3AI GATC 7 cut(s) 100, 307, 1048, 2251, 2278, 2473, 2948
Sau96I GGNCC 4 cut(s) 110, 592, 1191, 3215
ScaI AGTACT 2 cut(s) 1961, 2775
SchI GAGTC 4 cut(s) 1370, 2951, 3101, 3124
ScrFI CCNGG 8 cut(s) 63, 1299, 1623, 2033, 2592, 2711, 2804, 2850
SfcI CTRYAG 3 cut(s) 192, 1683, 1823
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 753
SinI GGWCC 3 cut(s) 110, 1191, 3215
SlaI CTCGAG 1 cut(s) 753
SmiI ATTTAAAT 1 cut(s) 2424
SmiMI CAYNNNNRTG 5 cut(s) 315, 742, 2312, 2412, 3290
SmlI CTYRAG 5 cut(s) 753, 2360, 2937, 3132, 3345
SmoI CTYRAG 5 cut(s) 753, 2360, 2937, 3132, 3345
SnaBI TACGTA 1 cut(s) 1886
SpeI ACTAGT 1 cut(s) 3200
SseBI AGGCCT 1 cut(s) 56
SsiI CCGC 7 cut(s) 113, 139, 1083, 1453, 2131, 2501, 2579
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 5 cut(s) 211, 542, 1495, 2374, 3088
SspMI CTAG 3 cut(s) 2913, 3111, 3201
SstI GAGCTC 2 cut(s) 722, 3073
StuI AGGCCT 1 cut(s) 56
StyD4I CCNGG 8 cut(s) 61, 1297, 1621, 2031, 2590, 2709, 2802, 2848
StyI CCWWGG 4 cut(s) 630, 1008, 1070, 1931
SwaI ATTTAAAT 1 cut(s) 2424
TaiI ACGT 7 cut(s) 391, 1576, 1741, 1888, 1901, 2194, 3385
TaqI TCGA 5 cut(s) 413, 754, 1333, 1379, 1693
TaqII GACCGA 1 cut(s) 1603
TatI WGTACW 5 cut(s) 1959, 2773, 2779, 3311, 3405
TseFI GTSAC 1 cut(s) 3364
TseI GCWGC 4 cut(s) 188, 638, 733, 3008
Tsp45I GTSAC 1 cut(s) 3364
TspGWI ACGGA 2 cut(s) 3169, 3251
Van91I CCANNNNNTGG 1 cut(s) 1097
Vha464I CTTAAG 1 cut(s) 2360
VneI GTGCAC 2 cut(s) 1894, 2568
VpaK11BI GGWCC 3 cut(s) 110, 1191, 3215
VspI ATTAAT 1 cut(s) 2700
XapI RAATTY 8 cut(s) 43, 1833, 2300, 2387, 2419, 2425, 2887, 3414
XceI RCATGY 4 cut(s) 424, 975, 2350, 3312
XhoI CTCGAG 1 cut(s) 753
XmiI GTMKAC 2 cut(s) 1693, 1735
XmnI GAANNNNTTC 2 cut(s) 993, 1222
XspI CTAG 3 cut(s) 2913, 3111, 3201
ZrmI AGTACT 2 cut(s) 1961, 2775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.