FvH4_6g08910

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008869
Physical Location & Seq
Reverse (-)
5285018 .. 5287922
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g08910.t3

Sequence Viewer

Length: 861 bp
ATGAGTATGCTGACGCTGGCAAGACCATGGAGGCTCTTTATGCATGTGTACAGGATGATGTTGGAACAGGAACATTGTGCCCCCAATGCTTACACTTACAGTGTTATCATCAAAACATTGGCTGCAGATCCGGAGCCCAATTTTCTTGGGGTTGTTAAGGAATGTGTGATGGAAATGATGGGGAAGGGGATGCAGCCCAATGCCAAGACTTATACGGCCGTTCTTGAGGCGTATGCAGGGCATGCGAAGATGGAGGAGGGCAAGGAGTTTTTGGAGGAGATGAAAGCTAATGGGTTTGTGGCTGACAAGATGGCAATGAGGGCTATGAGACGTGCTCTTAGGGGTAAACGACCTCTGGTTAAAATCATCATCAACATTCTCTTTGATAACTCCGAAGACTATTCAGGTGACTCAGAAGAAGATGATGAAGATATAAAGAAGTCAGACAATTTGATGGAACGTTTTAACATCCCTCGCTATGTTGGCAACAAACGTGTCATTGAAGACGACCCAGAAAACCCCAAAGATTTGGAATGTGTCTACGACATGATGTGCAGGATGGAACCCATCGTCCACACTATCACCAGGATGGTAAACATGCTAAAGAAAGATGGATTCATGTCCCAAGCCCATGAGATCAGGTCTGAGTTTGACTTGGGGGGTGATATACCTCAAGTTGTGACTCATACAGGCGTCATGGAGATCTATGCCAAGAGTGATCGAGCCAAGGAGGCTCATAAGGTTTTTCGGCCCCTTTTATCCCTCGGAGTTGTCCCAAATGCATACACTTATAGTATTATCATCAAGGCACTTGCTAAAGACTCTGATTTCCTTGAAGATGCTAAGACATGCTGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

287

Amino Acids

32.83

Weight (kDa)

5.74

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 224 - 273 2.7e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 540
AccIII TCCGGA 1 cut(s) 130
AclI AACGTT 1 cut(s) 460
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 1 cut(s) 216
AcyI GRCGYC 1 cut(s) 693
AfaI GTAC 1 cut(s) 50
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 2 cut(s) 503, 836
AjiI CACGTC 1 cut(s) 332
AjnI CCWGG 1 cut(s) 584
AloI GAACNNNNNNTCC 2 cut(s) 555, 587
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 1 cut(s) 122
Aor13HI TCCGGA 1 cut(s) 130
AoxI GGCC 2 cut(s) 216, 749
ApeKI GCWGC 2 cut(s) 122, 193
AspS9I GGNCC 1 cut(s) 750
AsuHPI GGTGA 3 cut(s) 419, 574, 674
BaeGI GKGCMC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 2 cut(s) 402, 510
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 2 cut(s) 109, 205
BccI CCATC 9 cut(s) 163, 172, 244, 304, 448, 553, 575, 583, 605
BceAI ACGGC 2 cut(s) 203, 231
BciT130I CCWGG 1 cut(s) 586
BcoDI GTCTC 1 cut(s) 322
BfmI CTRYAG 1 cut(s) 123
BglI GCCNNNNNGGC 1 cut(s) 731
BglII AGATCT 1 cut(s) 702
BisI GCNGC 2 cut(s) 123, 194
BlsI GCNGC 2 cut(s) 124, 195
Bme1390I CCNGG 1 cut(s) 586
BmgBI CACGTC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 750
BmiI GGNNCC 3 cut(s) 135, 564, 752
BmrFI CCNGG 1 cut(s) 586
BmsI GCATC 2 cut(s) 180, 829
BpiI GAAGAC 2 cut(s) 402, 510
BplI GAGNNNNNCTC 2 cut(s) 319, 351
BpuEI CTTGAG 2 cut(s) 245, 657
BsaHI GRCGYC 1 cut(s) 693
BsaJI CCNNGG 3 cut(s) 26, 726, 763
BsaWI WCCGGW 1 cut(s) 130
Bse3DI GCAATG 1 cut(s) 321
BseAI TCCGGA 1 cut(s) 130
BseBI CCWGG 1 cut(s) 586
BseDI CCNNGG 3 cut(s) 26, 726, 763
BseGI GGATG 5 cut(s) 60, 195, 468, 564, 594
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 2 cut(s) 426, 636
BseRI GAGGAG 2 cut(s) 269, 290
BseSI GKGCMC 1 cut(s) 82
BseX3I CGGCCG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 109, 205
BsgI GTGCAG 1 cut(s) 574
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 218, 751
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 2 cut(s) 607, 758
BsmAI GTCTC 1 cut(s) 322
BsmBI CGTCTC 1 cut(s) 322
BsmFI GGGAC 2 cut(s) 607, 758
BsnI GGCC 2 cut(s) 218, 751
Bsp1286I GDGCHC 3 cut(s) 82, 138, 337
Bsp13I TCCGGA 1 cut(s) 130
Bsp1407I TGTACA 1 cut(s) 48
Bsp143I GATC 4 cut(s) 127, 636, 702, 718
Bsp19I CCATGG 1 cut(s) 26
BspANI GGCC 2 cut(s) 218, 751
BspCNI CTCAG 2 cut(s) 425, 637
BspEI TCCGGA 1 cut(s) 130
BspLI GGNNCC 3 cut(s) 135, 564, 752
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 321
BsrGI TGTACA 1 cut(s) 48
BssECI CCNNGG 3 cut(s) 26, 726, 763
BssMI GATC 4 cut(s) 127, 636, 702, 718
BssNI GRCGYC 1 cut(s) 693
BssT1I CCWWGG 2 cut(s) 26, 726
Bst2UI CCWGG 1 cut(s) 586
Bst4CI ACNGT 1 cut(s) 101
BstACI GRCGYC 1 cut(s) 693
BstAPI GCANNNNNTGC 1 cut(s) 242
BstAUI TGTACA 1 cut(s) 48
BstC8I GCNNGC 2 cut(s) 18, 243
BstDEI CTNAG 4 cut(s) 338, 412, 645, 843
BstDSI CCRYGG 1 cut(s) 26
BstF5I GGATG 5 cut(s) 60, 195, 468, 564, 594
BstKTI GATC 4 cut(s) 130, 639, 705, 721
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 4 cut(s) 127, 636, 702, 718
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 6 cut(s) 40, 86, 242, 320, 483, 731
BstNI CCWGG 1 cut(s) 586
BstNSI RCATGY 4 cut(s) 47, 245, 601, 852
BstSCI CCNGG 1 cut(s) 584
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 109, 205
BstV2I GAAGAC 2 cut(s) 402, 510
BstX2I RGATCY 2 cut(s) 127, 702
BstXI CCANNNNNNTGG 1 cut(s) 529
BstYI RGATCY 2 cut(s) 127, 702
BstZI CGGCCG 1 cut(s) 216
BsuRI GGCC 2 cut(s) 218, 751
BtgI CCRYGG 1 cut(s) 26
BtrI CACGTC 1 cut(s) 332
BtsCI GGATG 5 cut(s) 60, 195, 468, 564, 594
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 18, 243
Cfr13I GGNCC 1 cut(s) 750
CseI GACGC 2 cut(s) 22, 682
Csp6I GTAC 1 cut(s) 49
CviAII CATG 9 cut(s) 27, 44, 242, 547, 598, 619, 632, 697, 849
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 4 cut(s) 338, 412, 645, 843
DpnI GATC 4 cut(s) 129, 638, 704, 720
DpnII GATC 4 cut(s) 127, 636, 702, 718
EaeI YGGCCR 1 cut(s) 216
EagI CGGCCG 1 cut(s) 216
EclXI CGGCCG 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 26, 726
Eco24I GRGCYC 1 cut(s) 138
Eco52I CGGCCG 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 584
EcoT14I CCWWGG 2 cut(s) 26, 726
EcoT22I ATGCAT 2 cut(s) 45, 784
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 2 cut(s) 26, 726
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 9 cut(s) 30, 47, 245, 550, 601, 622, 635, 700, 852
FaqI GGGAC 2 cut(s) 607, 758
FatI CATG 9 cut(s) 26, 43, 241, 546, 597, 618, 631, 696, 848
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 2 cut(s) 123, 194
FokI GGATG 5 cut(s) 67, 202, 455, 571, 601
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 123, 194
GluI GCNGC 2 cut(s) 123, 194
HaeIII GGCC 2 cut(s) 218, 751
HapII CCGG 1 cut(s) 131
HgaI GACGC 2 cut(s) 22, 682
Hin1I GRCGYC 1 cut(s) 693
Hin1II CATG 9 cut(s) 30, 47, 245, 550, 601, 622, 635, 700, 852
HinfI GANTC 4 cut(s) 410, 615, 682, 821
HpaII CCGG 1 cut(s) 131
HphI GGTGA 3 cut(s) 419, 574, 674
Hpy166II GTNNAC 5 cut(s) 49, 347, 541, 574, 595
Hpy188I TCNGA 6 cut(s) 394, 415, 445, 646, 767, 826
Hpy188III TCNNGA 2 cut(s) 131, 224
Hpy8I GTNNAC 5 cut(s) 49, 347, 541, 574, 595
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 3 cut(s) 331, 460, 493
HpyCH4V TGCA 6 cut(s) 43, 125, 193, 236, 555, 782
HpyF10VI GCNNNNNNNGC 6 cut(s) 40, 86, 242, 320, 483, 731
HpyF3I CTNAG 4 cut(s) 338, 412, 645, 843
HpySE526I ACGT 3 cut(s) 331, 460, 493
Hsp92I GRCGYC 1 cut(s) 693
Hsp92II CATG 9 cut(s) 30, 47, 245, 550, 601, 622, 635, 700, 852
Kpn2I TCCGGA 1 cut(s) 130
Kzo9I GATC 4 cut(s) 127, 636, 702, 718
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 2 cut(s) 109, 205
LweI GCATC 2 cut(s) 180, 829
MaeII ACGT 3 cut(s) 331, 460, 493
MaeIII GTNAC 2 cut(s) 407, 679
MalI GATC 4 cut(s) 129, 638, 704, 720
MboI GATC 4 cut(s) 127, 636, 702, 718
MboII GAAGA 7 cut(s) 259, 407, 428, 431, 440, 515, 848
MflI RGATCY 2 cut(s) 127, 702
MhlI GDGCHC 3 cut(s) 82, 138, 337
MluCI AATT 2 cut(s) 139, 448
MlyI GAGTC 3 cut(s) 404, 676, 815
MmeI TCCRAC 1 cut(s) 42
Mph1103I ATGCAT 2 cut(s) 45, 784
MroI TCCGGA 1 cut(s) 130
MseI TTAA 3 cut(s) 156, 360, 465
MslI CAYNNNNRTG 1 cut(s) 587
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 586
MvaI CCWGG 1 cut(s) 586
MwoI GCNNNNNNNGC 6 cut(s) 40, 86, 242, 320, 483, 731
NcoI CCATGG 1 cut(s) 26
NdeII GATC 4 cut(s) 127, 636, 702, 718
NlaIII CATG 9 cut(s) 30, 47, 245, 550, 601, 622, 635, 700, 852
NlaIV GGNNCC 3 cut(s) 135, 564, 752
NmuCI GTSAC 2 cut(s) 407, 679
NsiI ATGCAT 2 cut(s) 45, 784
NspI RCATGY 4 cut(s) 47, 245, 601, 852
PaeI GCATGC 1 cut(s) 245
PfeI GAWTC 1 cut(s) 615
PkrI GCNGC 2 cut(s) 124, 195
PleI GAGTC 3 cut(s) 404, 676, 815
PpsI GAGTC 3 cut(s) 404, 676, 815
Psp1406I AACGTT 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 584
PspGI CCWGG 1 cut(s) 584
PspN4I GGNNCC 3 cut(s) 135, 564, 752
PspPI GGNCC 1 cut(s) 750
PstI CTGCAG 1 cut(s) 127
PsuI RGATCY 2 cut(s) 127, 702
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 587
SaqAI TTAA 3 cut(s) 156, 360, 465
SatI GCNGC 2 cut(s) 123, 194
Sau3AI GATC 4 cut(s) 127, 636, 702, 718
Sau96I GGNCC 1 cut(s) 750
SchI GAGTC 3 cut(s) 404, 676, 815
ScrFI CCNGG 1 cut(s) 586
SduI GDGCHC 3 cut(s) 82, 138, 337
SetI ASST 9 cut(s) 289, 334, 355, 409, 463, 496, 644, 673, 744
SfaNI GCATC 2 cut(s) 180, 829
SfcI CTRYAG 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 2 cut(s) 224, 672
SmoI CTYRAG 2 cut(s) 224, 672
SphI GCATGC 1 cut(s) 245
Sse9I AATT 2 cut(s) 139, 448
StyD4I CCNGG 1 cut(s) 584
StyI CCWWGG 2 cut(s) 26, 726
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 3 cut(s) 334, 463, 496
TaqI TCGA 1 cut(s) 721
TasI AATT 2 cut(s) 139, 448
TatI WGTACW 1 cut(s) 48
TfiI GAWTC 1 cut(s) 615
Tru1I TTAA 3 cut(s) 156, 360, 465
Tru9I TTAA 3 cut(s) 156, 360, 465
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 2 cut(s) 407, 679
TseI GCWGC 2 cut(s) 122, 193
Tsp45I GTSAC 2 cut(s) 407, 679
TspDTI ATGAA 3 cut(s) 296, 441, 607
TspRI CASTG 1 cut(s) 106
XceI RCATGY 4 cut(s) 47, 245, 601, 852
XmiI GTMKAC 1 cut(s) 540
Zsp2I ATGCAT 2 cut(s) 45, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.