RLG00000025104

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008869
Physical Location & Seq
Forward (+)
42005255 .. 42007120
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025104

Sequence Viewer

Length: 1476 bp
ATGGCAAGCTCAAGCTCAAGCTCAAGGTCAAGGTCCAAAACGAAAACCAAAAACCAGACTATGATTAAGGTTAACAGCCCCCACATTGGTAGCATTGTGTTTGACAGTAGCAGCCTTCCTTCATCTCTTTGGGAAGACGGCTACAGGCCAGGCATCGTGGCTGGCCGAGGGGACCCGGAGGACTTGCCTGAAGTTTTCGACAAGGTGATGAACAGCCCTGTAGGCTTTATGAAGAATTTTGTGAAGATGTTTGATAACCTGTTAACAGACGGCTACAAGGACTTGGCTATGGAGATTGGGAATAGTCTTACGGAAGAGGGTGTTATACCCCAGATTGTGATCTTGACAGGGATTATTGATGAGTATGCTGAGGCTGGCAAGACCATGGAGGCTCTTTATGTGTACAGGATGATTTGGGAACATTGTGCCCTCAATGCTTACACTTACAGTGTTATCATCAAAGCACTAGCTGCGGATCCGGAGCCCAATTTTCTTGGGGTTGCCAAGGAATGTGTGATGGAGATGATGGAGAAGGGAATGCAGCCCAATGCCAAGACTTACACGGCAGTGCTTGAGGCGTTTGCAGGGCATGAGAAGATGGAGGAGGGCAAGATGTTTCTGCAGGAGATGAAAGCTAATGGGTTTGTGGCAGACAGGAAGGCAATGATGGCTATGAGAAATGTTCTCAAGAGTAAACGATCTCTGGTCAAAACCATCATTGAGATTCTGTTTGGATTGCTGTTCTTTACTAACTTTGACAAGTTGTTGGCTGTAGATAACTCGGAAGACTATTCAGGTGACTCAGAAGATGATGATGATGCTATAAAGAAGTCCAACAAAATGATGGAATATTTTAACTGCCCTCCTGATCGCTATATTGGCAACAAACACGTCATTGAGGACGACCCAGAAAACCCCAAAGATTTGGAACAAGTCTTCGACATAATGTGCATGACAGAACCCCTCATCCACACTTTCACCAGGATGGTGAACATGCTAAAAAAAGATGGATTTCTGTCCCAAGCTCAGGAGATAAGGGCTGAGTTTGACTTGGGAGGAGATATACCTGAAGTCGTGACCCACACCGGCGTCATTGAGATCTATGCCAAGAGTGATCAAGCCAAGGAGGCTCACAAGGTTTTCCTGCGCATGTTATCCCTCGGAGTCGCTCCAAATGCATACACTTATAGTGTTATCATTAAGGCACTTGCTAAAGACTCTAACTTCCTTGAAGATGCTAAGAAGTACTTGCTGGAAATGATGGATAAGGGAATGCAGCCCAATGCCAGGACATACACCCCTGTGTTCAAAGCCTTTGCCCGGCTGGAGGAGGATAATGTGAAGAAAGGGAAGGAGTTTCTGATGGAGATGAAAGCTAAGGGATTCAGGGCTAAAAAGAAGGCTGTGATGGAGGTTCTCAAGGGAAGAAAAGGACCAGTGGTTGAAACGGTTATCAGTATTCTCTTCGGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

492

Amino Acids

55.21

Weight (kDa)

5.64

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 356 - 405 1.4e-11 PPR repeat family
PPR_3 PF13812 356 - 403 9.9e-06 Pentatricopeptide repeat domain
TPR_24 PF23276 362 - 442 8.5e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 371 - 474 1.6e-09 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1148
AccIII TCCGGA 1 cut(s) 478
AciI CCGC 1 cut(s) 473
AclWI GGATC 2 cut(s) 470, 483
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 2 cut(s) 210, 1089
AcyI GRCGYC 1 cut(s) 1089
AfaI GTAC 2 cut(s) 404, 1247
AfiI CCNNNNNNNGG 5 cut(s) 86, 1027, 1287, 1320, 1327
AflIII ACRYGT 1 cut(s) 889
AgsI TTSAA 3 cut(s) 1232, 1309, 1445
AjiI CACGTC 1 cut(s) 892
AjnI CCWGG 3 cut(s) 148, 980, 1286
AleI CACNNNNGTG 2 cut(s) 566, 1301
AloI GAACNNNNNNTCC 2 cut(s) 951, 983
AluBI AGCT 7 cut(s) 9, 15, 21, 470, 635, 1025, 1376
AluI AGCT 7 cut(s) 9, 15, 21, 470, 635, 1025, 1376
AlwI GGATC 2 cut(s) 470, 483
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 2 cut(s) 146, 163
ApeKI GCWGC 4 cut(s) 111, 470, 541, 1276
ApoI RAATTY 1 cut(s) 235
AspLEI GCGC 1 cut(s) 1149
AspS9I GGNCC 3 cut(s) 33, 172, 1433
AsuC2I CCSGG 2 cut(s) 176, 1321
AsuHPI GGTGA 4 cut(s) 217, 809, 970, 1000
AvaII GGWCC 3 cut(s) 33, 172, 1433
BaeGI GKGCMC 1 cut(s) 430
BamHI GGATCC 1 cut(s) 475
BanII GRGCYC 1 cut(s) 486
BbsI GAAGAC 3 cut(s) 141, 792, 928
BbvCI CCTCAGC 1 cut(s) 369
BbvI GCAGC 4 cut(s) 123, 457, 553, 1288
BceAI ACGGC 3 cut(s) 154, 286, 579
BciT130I CCWGG 3 cut(s) 150, 982, 1288
BclI TGATCA 1 cut(s) 1114
BcnI CCSGG 2 cut(s) 176, 1321
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 4 cut(s) 142, 219, 620, 771
BglI GCCNNNNNGGC 2 cut(s) 222, 1127
BglII AGATCT 1 cut(s) 1098
BisI GCNGC 4 cut(s) 112, 471, 542, 1277
BlsI GCNGC 4 cut(s) 113, 472, 543, 1278
BmcAI AGTACT 1 cut(s) 1247
Bme1390I CCNGG 5 cut(s) 150, 176, 982, 1288, 1321
Bme18I GGWCC 3 cut(s) 33, 172, 1433
BmgBI CACGTC 1 cut(s) 892
BmgT120I GGNCC 3 cut(s) 33, 172, 1433
BmiI GGNNCC 4 cut(s) 173, 174, 477, 483
BmrFI CCNGG 5 cut(s) 150, 176, 982, 1288, 1321
BmsI GCATC 3 cut(s) 162, 808, 1225
BpiI GAAGAC 3 cut(s) 141, 792, 928
BpmI CTGGAG 1 cut(s) 1346
Bpu10I CCTNAGC 3 cut(s) 369, 1026, 1377
BpuEI CTTGAG 4 cut(s) 7, 593, 671, 1403
BpuMI CCSGG 2 cut(s) 176, 1321
BsaBI GATNNNNATC 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 1089
BsaJI CCNNGG 5 cut(s) 166, 384, 504, 1122, 1159
BsaWI WCCGGW 1 cut(s) 478
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 1027, 1287, 1320, 1327
Bse118I RCCGGY 1 cut(s) 1085
Bse1I ACTGG 1 cut(s) 1436
Bse3DI GCAATG 1 cut(s) 669
Bse8I GATNNNNATC 1 cut(s) 338
BseAI TCCGGA 1 cut(s) 478
BseBI CCWGG 3 cut(s) 150, 982, 1288
BseDI CCNNGG 5 cut(s) 166, 384, 504, 1122, 1159
BseGI GGATG 3 cut(s) 414, 966, 990
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 5 cut(s) 86, 1027, 1287, 1320, 1327
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 4 cut(s) 360, 816, 1032, 1040
BseNI ACTGG 1 cut(s) 1436
BseRI GAGGAG 3 cut(s) 617, 1071, 1343
BseSI GKGCMC 1 cut(s) 430
BseXI GCAGC 4 cut(s) 123, 457, 553, 1288
BshFI GGCC 2 cut(s) 148, 165
BsiSI CCGG 4 cut(s) 176, 479, 1086, 1321
BslFI GGGAC 2 cut(s) 185, 1003
BslI CCNNNNNNNGG 5 cut(s) 86, 1027, 1287, 1320, 1327
BsmFI GGGAC 2 cut(s) 185, 1003
BsmI GAATGC 2 cut(s) 543, 1278
BsnI GGCC 2 cut(s) 148, 165
Bsp1286I GDGCHC 2 cut(s) 430, 486
Bsp13I TCCGGA 1 cut(s) 478
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 6 cut(s) 339, 475, 698, 868, 1098, 1114
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 1 cut(s) 473
BspANI GGCC 2 cut(s) 148, 165
BspCNI CTCAG 4 cut(s) 361, 815, 1033, 1039
BspEI TCCGGA 1 cut(s) 478
BspLI GGNNCC 4 cut(s) 173, 174, 477, 483
BspMAI CTGCAG 1 cut(s) 624
BspPI GGATC 2 cut(s) 470, 483
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 1085
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 1 cut(s) 1436
BssAI RCCGGY 1 cut(s) 1085
BssECI CCNNGG 5 cut(s) 166, 384, 504, 1122, 1159
BssMI GATC 6 cut(s) 339, 475, 698, 868, 1098, 1114
BssNI GRCGYC 1 cut(s) 1089
BssT1I CCWWGG 3 cut(s) 384, 504, 1122
Bst2UI CCWGG 3 cut(s) 150, 982, 1288
Bst4CI ACNGT 3 cut(s) 107, 449, 1450
Bst6I CTCTTC 2 cut(s) 309, 1469
BstACI GRCGYC 1 cut(s) 1089
BstAPI GCANNNNNTGC 1 cut(s) 470
BstAUI TGTACA 1 cut(s) 402
BstC8I GCNNGC 3 cut(s) 7, 163, 376
BstDEI CTNAG 6 cut(s) 369, 802, 1026, 1041, 1239, 1377
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 3 cut(s) 414, 966, 990
BstHHI GCGC 1 cut(s) 1149
BstKTI GATC 6 cut(s) 342, 478, 701, 871, 1101, 1117
BstMBI GATC 6 cut(s) 339, 475, 698, 868, 1098, 1114
BstMWI GCNNNNNNNGC 7 cut(s) 222, 434, 470, 668, 879, 1127, 1175
BstNI CCWGG 3 cut(s) 150, 982, 1288
BstNSI RCATGY 2 cut(s) 997, 1153
BstSCI CCNGG 5 cut(s) 148, 174, 980, 1286, 1319
BstSFI CTRYAG 4 cut(s) 142, 219, 620, 771
BstSLI GKGCMC 1 cut(s) 430
BstV1I GCAGC 4 cut(s) 123, 457, 553, 1288
BstV2I GAAGAC 3 cut(s) 141, 792, 928
BstX2I RGATCY 2 cut(s) 475, 1098
BstXI CCANNNNNNTGG 1 cut(s) 925
BstYI RGATCY 2 cut(s) 475, 1098
BsuRI GGCC 2 cut(s) 148, 165
BtgI CCRYGG 1 cut(s) 384
BtrI CACGTC 1 cut(s) 892
BtsCI GGATG 3 cut(s) 414, 966, 990
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 3 cut(s) 454, 573, 1443
Cac8I GCNNGC 3 cut(s) 7, 163, 376
CfoI GCGC 1 cut(s) 1149
Cfr10I RCCGGY 1 cut(s) 1085
Cfr13I GGNCC 3 cut(s) 33, 172, 1433
CseI GACGC 1 cut(s) 1078
Csp6I GTAC 2 cut(s) 403, 1246
CviAII CATG 5 cut(s) 385, 590, 952, 994, 1150
CviQI GTAC 2 cut(s) 403, 1246
DdeI CTNAG 6 cut(s) 369, 802, 1026, 1041, 1239, 1377
DpnI GATC 6 cut(s) 341, 477, 700, 870, 1100, 1116
DpnII GATC 6 cut(s) 339, 475, 698, 868, 1098, 1114
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 309, 1469
EarI CTCTTC 2 cut(s) 309, 1469
Eco130I CCWWGG 3 cut(s) 384, 504, 1122
Eco24I GRGCYC 1 cut(s) 486
Eco47I GGWCC 3 cut(s) 33, 172, 1433
Eco57I CTGAAG 2 cut(s) 210, 1089
EcoO109I RGGNCCY 1 cut(s) 172
EcoRII CCWGG 3 cut(s) 148, 980, 1286
EcoT14I CCWWGG 3 cut(s) 384, 504, 1122
EcoT22I ATGCAT 1 cut(s) 1180
EcoT38I GRGCYC 1 cut(s) 486
ErhI CCWWGG 3 cut(s) 384, 504, 1122
FaeI CATG 5 cut(s) 388, 593, 955, 997, 1153
FalI AAGNNNNNCTT 2 cut(s) 1232, 1264
FaqI GGGAC 2 cut(s) 185, 1003
FatI CATG 5 cut(s) 384, 589, 951, 993, 1149
FbaI TGATCA 1 cut(s) 1114
Fnu4HI GCNGC 4 cut(s) 112, 471, 542, 1277
FokI GGATG 3 cut(s) 421, 953, 997
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 4 cut(s) 112, 471, 542, 1277
FspBI CTAG 1 cut(s) 467
FspI TGCGCA 1 cut(s) 1148
GlaI GCGC 1 cut(s) 1148
GluI GCNGC 4 cut(s) 112, 471, 542, 1277
GsuI CTGGAG 1 cut(s) 1346
HaeIII GGCC 2 cut(s) 148, 165
HapII CCGG 4 cut(s) 176, 479, 1086, 1321
HgaI GACGC 1 cut(s) 1078
HhaI GCGC 1 cut(s) 1149
Hin1I GRCGYC 1 cut(s) 1089
Hin1II CATG 5 cut(s) 388, 593, 955, 997, 1153
Hin6I GCGC 1 cut(s) 1147
HinP1I GCGC 1 cut(s) 1147
HincII GTYRAC 2 cut(s) 73, 264
HindII GTYRAC 2 cut(s) 73, 264
HinfI GANTC 5 cut(s) 724, 800, 1164, 1217, 1383
HpaI GTTAAC 2 cut(s) 73, 264
HpaII CCGG 4 cut(s) 176, 479, 1086, 1321
HphI GGTGA 4 cut(s) 217, 809, 970, 1000
Hpy166II GTNNAC 5 cut(s) 73, 264, 403, 695, 991
Hpy188I TCNGA 4 cut(s) 784, 805, 1163, 1362
Hpy188III TCNNGA 6 cut(s) 343, 479, 688, 866, 1028, 1075
Hpy8I GTNNAC 5 cut(s) 73, 264, 403, 695, 991
HpyAV CCTTC 6 cut(s) 125, 129, 526, 652, 1345, 1393
HpyCH4III ACNGT 3 cut(s) 107, 449, 1450
HpyCH4IV ACGT 1 cut(s) 891
HpyCH4V TGCA 6 cut(s) 541, 584, 622, 951, 1178, 1276
HpyF10VI GCNNNNNNNGC 7 cut(s) 222, 434, 470, 668, 879, 1127, 1175
HpyF3I CTNAG 6 cut(s) 369, 802, 1026, 1041, 1239, 1377
HpySE526I ACGT 1 cut(s) 891
Hsp92I GRCGYC 1 cut(s) 1089
Hsp92II CATG 5 cut(s) 388, 593, 955, 997, 1153
HspAI GCGC 1 cut(s) 1147
KflI GGGWCCC 1 cut(s) 172
Kpn2I TCCGGA 1 cut(s) 478
Ksp22I TGATCA 1 cut(s) 1114
KspAI GTTAAC 2 cut(s) 73, 264
Kzo9I GATC 6 cut(s) 339, 475, 698, 868, 1098, 1114
LmnI GCTCC 2 cut(s) 481, 1174
Lsp1109I GCAGC 4 cut(s) 123, 457, 553, 1288
LweI GCATC 3 cut(s) 162, 808, 1225
MaeI CTAG 1 cut(s) 467
MaeII ACGT 1 cut(s) 891
MaeIII GTNAC 2 cut(s) 797, 1075
MalI GATC 6 cut(s) 341, 477, 700, 870, 1100, 1116
MboI GATC 6 cut(s) 339, 475, 698, 868, 1098, 1114
MflI RGATCY 2 cut(s) 475, 1098
MhlI GDGCHC 2 cut(s) 430, 486
MluCI AATT 2 cut(s) 235, 487
MlyI GAGTC 3 cut(s) 794, 1173, 1211
MmeI TCCRAC 1 cut(s) 858
Mph1103I ATGCAT 1 cut(s) 1180
MroI TCCGGA 1 cut(s) 478
MseI TTAA 5 cut(s) 66, 72, 263, 855, 1200
MslI CAYNNNNRTG 3 cut(s) 566, 983, 1301
MspI CCGG 4 cut(s) 176, 479, 1086, 1321
MspR9I CCNGG 5 cut(s) 150, 176, 982, 1288, 1321
Mva1269I GAATGC 2 cut(s) 543, 1278
MvaI CCWGG 3 cut(s) 150, 982, 1288
MwoI GCNNNNNNNGC 7 cut(s) 222, 434, 470, 668, 879, 1127, 1175
NciI CCSGG 2 cut(s) 176, 1321
NcoI CCATGG 1 cut(s) 384
NdeII GATC 6 cut(s) 339, 475, 698, 868, 1098, 1114
NlaIII CATG 5 cut(s) 388, 593, 955, 997, 1153
NlaIV GGNNCC 4 cut(s) 173, 174, 477, 483
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 2 cut(s) 797, 1075
NsbI TGCGCA 1 cut(s) 1148
NsiI ATGCAT 1 cut(s) 1180
NspI RCATGY 2 cut(s) 997, 1153
OliI CACNNNNGTG 2 cut(s) 566, 1301
PctI GAATGC 2 cut(s) 543, 1278
PfeI GAWTC 2 cut(s) 724, 1383
PkrI GCNGC 4 cut(s) 113, 472, 543, 1278
PleI GAGTC 3 cut(s) 794, 1172, 1211
PpsI GAGTC 3 cut(s) 794, 1172, 1211
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 3 cut(s) 148, 980, 1286
PspGI CCWGG 3 cut(s) 148, 980, 1286
PspN4I GGNNCC 4 cut(s) 173, 174, 477, 483
PspPI GGNCC 3 cut(s) 33, 172, 1433
PspPPI RGGWCCY 1 cut(s) 172
PstI CTGCAG 1 cut(s) 624
PsuI RGATCY 2 cut(s) 475, 1098
RsaI GTAC 2 cut(s) 404, 1247
RsaNI GTAC 2 cut(s) 403, 1246
RseI CAYNNNNRTG 3 cut(s) 566, 983, 1301
SaqAI TTAA 5 cut(s) 66, 72, 263, 855, 1200
SatI GCNGC 4 cut(s) 112, 471, 542, 1277
Sau3AI GATC 6 cut(s) 339, 475, 698, 868, 1098, 1114
Sau96I GGNCC 3 cut(s) 33, 172, 1433
ScaI AGTACT 1 cut(s) 1247
SchI GAGTC 3 cut(s) 794, 1173, 1211
ScrFI CCNGG 5 cut(s) 150, 176, 982, 1288, 1321
SduI GDGCHC 2 cut(s) 430, 486
SfaNI GCATC 3 cut(s) 162, 808, 1225
SfcI CTRYAG 4 cut(s) 142, 219, 620, 771
SgrAI CRCCGGYG 1 cut(s) 1085
SinI GGWCC 3 cut(s) 33, 172, 1433
SmiMI CAYNNNNRTG 3 cut(s) 566, 983, 1301
SmlI CTYRAG 6 cut(s) 10, 16, 22, 572, 686, 1418
SmoI CTYRAG 6 cut(s) 10, 16, 22, 572, 686, 1418
Sse9I AATT 2 cut(s) 235, 487
SsiI CCGC 1 cut(s) 473
SspI AATATT 1 cut(s) 851
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 5 cut(s) 148, 174, 980, 1286, 1319
StyI CCWWGG 3 cut(s) 384, 504, 1122
TaaI ACNGT 3 cut(s) 107, 449, 1450
TaiI ACGT 1 cut(s) 894
TaqI TCGA 2 cut(s) 198, 939
TasI AATT 2 cut(s) 235, 487
TatI WGTACW 2 cut(s) 402, 1245
TfiI GAWTC 2 cut(s) 724, 1383
Tru1I TTAA 5 cut(s) 66, 72, 263, 855, 1200
Tru9I TTAA 5 cut(s) 66, 72, 263, 855, 1200
TscAI CASTG 3 cut(s) 454, 573, 1443
TseFI GTSAC 2 cut(s) 797, 1075
TseI GCWGC 4 cut(s) 111, 470, 541, 1276
Tsp45I GTSAC 2 cut(s) 797, 1075
TspDTI ATGAA 5 cut(s) 111, 224, 245, 644, 1385
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 3 cut(s) 454, 573, 1443
VpaK11BI GGWCC 3 cut(s) 33, 172, 1433
XapI RAATTY 1 cut(s) 235
XceI RCATGY 2 cut(s) 997, 1153
XcmI CCANNNNNNNNNTGG 1 cut(s) 841
XspI CTAG 1 cut(s) 467
ZrmI AGTACT 1 cut(s) 1247
Zsp2I ATGCAT 1 cut(s) 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.