Rh3AG092200

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0008869
Physical Location & Seq
Reverse (-)
7548389 .. 7551206
2818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG092200.1

Sequence Viewer

Length: 1434 bp
ATGGCAAGCTCAAGCTCAAGGTCAAGGTCAAGGTCCAAAACCAAAAACAAAACCCAGACTACGATTAAGGTTAACAGCCCCCACGTTGGTAGCATTGTGTTTGACAGTAGCAGCCTTCCTTCATCTCTTTGGGAAGACGGCTACAGGCCAGGCATCGTGGCTGGCCCAGGGGACCCGGAGGACTTGCCTGAAGTTTTCGACAAGGTGATGAACAGCCCTGAAGGCTTTATGAAGAATTTTGTGAAGATGTTTGATAACCTGTTAACAGACGGCTACAAGGACTTGGCTATGGAGATTGGGAATAGTCTTACGGAAGAGGGTGTTATACCCCAGATTGTGGTCGTGACAGGGATTATTGATGAGTATGCTGAGGCTGGCAAGACCATGGAGGCTCTTTATGTGTTCAGGATGATTTGGGAACATTGTGTCCTCAATGCTTACACTTACAGTGTTATCATCCAAGCACTAGCTGCGGATCCGGAGCCCAATTTTCTTGGGGTTGCCAAGGAATGTGTGATGGAGATGATGGGGAAGGGAATGCAGCCCAATGCCAAGACTTACACCGCCGTGCTTGAGGCGTATGCAGGGCATGAGAAGATGGAGGAGGGCAAGATGTTTCTGCAGGAGATGAAAGCTAATGGGTTTGTGGCAGACAGGAAGGCAATGAGGGCTATGAGAAATGTTCTCAAGAGTAAACGATCTCTGGTCAAAACCATCATTGAGATTCTGTTTGATAACTCGGAAGACTATTCAGGTGACTCAGAAGATGATGATGATGCTATAAAGAAGTCCAACAAAATGATGGAATATTTTAAATGCCCTCCTGATGGCTATGTTGGCAGCAAACACGTCATTGAGGACGATCCGGAAAACCCCAAAGATTTGGAACGAGTCTTCGACATAATGTGCAAGACAGAACCCCTCATCCACACTTTCACCAGGATGGTGAACATGCTAAAAAAAGATGGATTTCTGTCCCAAGCCCAGGAGATAAGGGCTGAGTTTGACTTGGGAGGACATATACCTGAAGTCGTGACCCACACCGGCGTCATTGAGATCTATGCCAAGAGTGATCAAGCCAAGGAGGCTCACAAGGTTTTCCTGCGCATGTTATCCCTCGGAGTTGCTCCAAATGCATACACTTATAGTGTTATCATTAAGGCACTTGCTAAAGACTCTAACTTCCTTGAAGATGCTAAGAAGTACTTGCTGGAAATGATGGATAAGGGAATGCAGCCTAATGCCAGGACATACACCCCTGTGTTCAAAGCCTTTGCCAGGCTGGAGGAGGATAATGTGAAGAAAGGGAAGGAGTTTCTGATGGAGATGAAAGCCAAGGGATTCAGGGCTAAAAAGAAGGCTGTGATGGAGGTTGTCAAGGGAAGAAAAGGACTAGTGGTTGAAACCGTTATCAGTATTCTCTTCGGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

477

Amino Acids

53.5

Weight (kDa)

6.03

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 342 - 391 1.3e-11 PPR repeat family
PPR_3 PF13812 342 - 389 7.1e-06 Pentatricopeptide repeat domain
TPR_24 PF23276 348 - 428 8.7e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 356 - 452 2.6e-09 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1106
AccB7I CCANNNNNTGG 1 cut(s) 337
AccIII TCCGGA 2 cut(s) 478, 865
AciI CCGC 2 cut(s) 473, 564
AclWI GGATC 3 cut(s) 470, 483, 857
AcsI RAATTY 1 cut(s) 235
AcuI CTGAAG 3 cut(s) 210, 240, 1047
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 1 cut(s) 1205
AfiI CCNNNNNNNGG 5 cut(s) 86, 337, 827, 985, 1278
AflIII ACRYGT 1 cut(s) 847
AgsI TTSAA 3 cut(s) 1190, 1267, 1403
AhlI ACTAGT 1 cut(s) 1393
AjiI CACGTC 1 cut(s) 850
AjnI CCWGG 6 cut(s) 148, 166, 938, 984, 1244, 1277
AleI CACNNNNGTG 2 cut(s) 566, 1259
AloI GAACNNNNNNTCC 4 cut(s) 411, 443, 909, 941
AluBI AGCT 4 cut(s) 9, 15, 470, 635
AluI AGCT 4 cut(s) 9, 15, 470, 635
AlwI GGATC 3 cut(s) 470, 483, 857
Aor13HI TCCGGA 2 cut(s) 478, 865
AoxI GGCC 2 cut(s) 146, 163
ApeKI GCWGC 5 cut(s) 111, 470, 541, 840, 1234
ApoI RAATTY 1 cut(s) 235
AspLEI GCGC 1 cut(s) 1107
AspS9I GGNCC 3 cut(s) 33, 164, 172
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 4 cut(s) 217, 767, 928, 958
AvaII GGWCC 2 cut(s) 33, 172
BamHI GGATCC 1 cut(s) 475
BanII GRGCYC 1 cut(s) 486
BbsI GAAGAC 3 cut(s) 141, 750, 886
BbvCI CCTCAGC 1 cut(s) 369
BbvI GCAGC 5 cut(s) 123, 457, 553, 852, 1246
BceAI ACGGC 3 cut(s) 154, 286, 551
BciT130I CCWGG 6 cut(s) 150, 168, 940, 986, 1246, 1279
BclI TGATCA 1 cut(s) 1072
BcnI CCSGG 1 cut(s) 176
BcuI ACTAGT 1 cut(s) 1393
BfaI CTAG 2 cut(s) 467, 1394
BfmI CTRYAG 2 cut(s) 142, 620
BglI GCCNNNNNGGC 2 cut(s) 222, 1085
BglII AGATCT 1 cut(s) 1056
BisI GCNGC 5 cut(s) 112, 471, 542, 841, 1235
BlsI GCNGC 5 cut(s) 113, 472, 543, 842, 1236
BmcAI AGTACT 1 cut(s) 1205
Bme1390I CCNGG 7 cut(s) 150, 168, 176, 940, 986, 1246, 1279
Bme18I GGWCC 2 cut(s) 33, 172
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 3 cut(s) 33, 164, 172
BmiI GGNNCC 4 cut(s) 173, 174, 477, 483
BmrFI CCNGG 7 cut(s) 150, 168, 176, 940, 986, 1246, 1279
BmsI GCATC 3 cut(s) 162, 766, 1183
BpiI GAAGAC 3 cut(s) 141, 750, 886
BpmI CTGGAG 1 cut(s) 1304
Bpu10I CCTNAGC 1 cut(s) 369
BpuEI CTTGAG 2 cut(s) 593, 671
BpuMI CCSGG 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 1047
BsaJI CCNNGG 8 cut(s) 166, 167, 384, 504, 984, 1080, 1117, 1335
BsaWI WCCGGW 2 cut(s) 478, 865
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 337, 827, 985, 1278
Bse118I RCCGGY 1 cut(s) 1043
Bse3DI GCAATG 1 cut(s) 669
BseAI TCCGGA 2 cut(s) 478, 865
BseBI CCWGG 6 cut(s) 150, 168, 940, 986, 1246, 1279
BseDI CCNNGG 8 cut(s) 166, 167, 384, 504, 984, 1080, 1117, 1335
BseGI GGATG 4 cut(s) 414, 456, 924, 948
BseLI CCNNNNNNNGG 5 cut(s) 86, 337, 827, 985, 1278
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 3 cut(s) 360, 774, 990
BseRI GAGGAG 2 cut(s) 617, 1301
BseXI GCAGC 5 cut(s) 123, 457, 553, 852, 1246
BshFI GGCC 2 cut(s) 148, 165
BsiSI CCGG 4 cut(s) 176, 479, 866, 1044
BslFI GGGAC 2 cut(s) 185, 961
BslI CCNNNNNNNGG 5 cut(s) 86, 337, 827, 985, 1278
BsmFI GGGAC 2 cut(s) 185, 961
BsmI GAATGC 2 cut(s) 543, 1236
BsnI GGCC 2 cut(s) 148, 165
Bsp1286I GDGCHC 1 cut(s) 486
Bsp13I TCCGGA 2 cut(s) 478, 865
Bsp143I GATC 5 cut(s) 475, 698, 862, 1056, 1072
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 2 cut(s) 473, 564
BspANI GGCC 2 cut(s) 148, 165
BspCNI CTCAG 3 cut(s) 361, 773, 991
BspEI TCCGGA 2 cut(s) 478, 865
BspLI GGNNCC 4 cut(s) 173, 174, 477, 483
BspMAI CTGCAG 1 cut(s) 624
BspPI GGATC 3 cut(s) 470, 483, 857
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 1043
BssAI RCCGGY 1 cut(s) 1043
BssECI CCNNGG 8 cut(s) 166, 167, 384, 504, 984, 1080, 1117, 1335
BssMI GATC 5 cut(s) 475, 698, 862, 1056, 1072
BssNI GRCGYC 1 cut(s) 1047
BssT1I CCWWGG 4 cut(s) 384, 504, 1080, 1335
Bst2UI CCWGG 6 cut(s) 150, 168, 940, 986, 1246, 1279
Bst4CI ACNGT 3 cut(s) 107, 449, 1408
Bst6I CTCTTC 2 cut(s) 309, 1427
BstACI GRCGYC 1 cut(s) 1047
BstAPI GCANNNNNTGC 1 cut(s) 470
BstC8I GCNNGC 3 cut(s) 7, 163, 376
BstDEI CTNAG 4 cut(s) 369, 760, 999, 1197
BstDSI CCRYGG 1 cut(s) 384
BstENI CCTNNNNNAGG 1 cut(s) 1276
BstF5I GGATG 4 cut(s) 414, 456, 924, 948
BstHHI GCGC 1 cut(s) 1107
BstKTI GATC 5 cut(s) 478, 701, 865, 1059, 1075
BstMBI GATC 5 cut(s) 475, 698, 862, 1056, 1072
BstMWI GCNNNNNNNGC 6 cut(s) 222, 470, 668, 837, 1085, 1133
BstNI CCWGG 6 cut(s) 150, 168, 940, 986, 1246, 1279
BstNSI RCATGY 2 cut(s) 955, 1111
BstSCI CCNGG 7 cut(s) 148, 166, 174, 938, 984, 1244, 1277
BstSFI CTRYAG 2 cut(s) 142, 620
BstV1I GCAGC 5 cut(s) 123, 457, 553, 852, 1246
BstV2I GAAGAC 3 cut(s) 141, 750, 886
BstX2I RGATCY 2 cut(s) 475, 1056
BstXI CCANNNNNNTGG 1 cut(s) 883
BstYI RGATCY 2 cut(s) 475, 1056
BsuRI GGCC 2 cut(s) 148, 165
BtgI CCRYGG 1 cut(s) 384
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 4 cut(s) 414, 456, 924, 948
BtsIMutI CAGTG 1 cut(s) 454
Cac8I GCNNGC 3 cut(s) 7, 163, 376
CfoI GCGC 1 cut(s) 1107
Cfr10I RCCGGY 1 cut(s) 1043
Cfr13I GGNCC 3 cut(s) 33, 164, 172
CseI GACGC 1 cut(s) 1036
Csp6I GTAC 1 cut(s) 1204
CviAII CATG 4 cut(s) 385, 590, 952, 1108
CviQI GTAC 1 cut(s) 1204
DdeI CTNAG 4 cut(s) 369, 760, 999, 1197
DpnI GATC 5 cut(s) 477, 700, 864, 1058, 1074
DpnII GATC 5 cut(s) 475, 698, 862, 1056, 1072
DraI TTTAAA 1 cut(s) 814
Eam1104I CTCTTC 2 cut(s) 309, 1427
EarI CTCTTC 2 cut(s) 309, 1427
Eco130I CCWWGG 4 cut(s) 384, 504, 1080, 1335
Eco24I GRGCYC 1 cut(s) 486
Eco47I GGWCC 2 cut(s) 33, 172
Eco57I CTGAAG 3 cut(s) 210, 240, 1047
EcoNI CCTNNNNNAGG 1 cut(s) 1276
EcoO109I RGGNCCY 1 cut(s) 172
EcoRII CCWGG 6 cut(s) 148, 166, 938, 984, 1244, 1277
EcoT14I CCWWGG 4 cut(s) 384, 504, 1080, 1335
EcoT22I ATGCAT 1 cut(s) 1138
EcoT38I GRGCYC 1 cut(s) 486
ErhI CCWWGG 4 cut(s) 384, 504, 1080, 1335
FaeI CATG 4 cut(s) 388, 593, 955, 1111
FalI AAGNNNNNCTT 2 cut(s) 1190, 1222
FaqI GGGAC 2 cut(s) 185, 961
FatI CATG 4 cut(s) 384, 589, 951, 1107
FbaI TGATCA 1 cut(s) 1072
Fnu4HI GCNGC 5 cut(s) 112, 471, 542, 841, 1235
FokI GGATG 4 cut(s) 421, 443, 911, 955
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 5 cut(s) 112, 471, 542, 841, 1235
FspBI CTAG 2 cut(s) 467, 1394
FspI TGCGCA 1 cut(s) 1106
GlaI GCGC 1 cut(s) 1106
GluI GCNGC 5 cut(s) 112, 471, 542, 841, 1235
GsuI CTGGAG 1 cut(s) 1304
HaeIII GGCC 2 cut(s) 148, 165
HapII CCGG 4 cut(s) 176, 479, 866, 1044
HgaI GACGC 1 cut(s) 1036
HhaI GCGC 1 cut(s) 1107
Hin1I GRCGYC 1 cut(s) 1047
Hin1II CATG 4 cut(s) 388, 593, 955, 1111
Hin6I GCGC 1 cut(s) 1105
HinP1I GCGC 1 cut(s) 1105
HincII GTYRAC 2 cut(s) 73, 264
HindII GTYRAC 2 cut(s) 73, 264
HinfI GANTC 5 cut(s) 724, 758, 891, 1175, 1341
HpaI GTTAAC 2 cut(s) 73, 264
HpaII CCGG 4 cut(s) 176, 479, 866, 1044
HphI GGTGA 4 cut(s) 217, 767, 928, 958
Hpy166II GTNNAC 4 cut(s) 73, 264, 695, 949
Hpy188I TCNGA 4 cut(s) 742, 763, 1121, 1320
Hpy188III TCNNGA 7 cut(s) 343, 406, 479, 688, 824, 866, 1033
Hpy8I GTNNAC 4 cut(s) 73, 264, 695, 949
HpyAV CCTTC 7 cut(s) 125, 129, 215, 526, 652, 1303, 1351
HpyCH4III ACNGT 3 cut(s) 107, 449, 1408
HpyCH4IV ACGT 2 cut(s) 84, 849
HpyCH4V TGCA 6 cut(s) 541, 584, 622, 909, 1136, 1234
HpyF10VI GCNNNNNNNGC 6 cut(s) 222, 470, 668, 837, 1085, 1133
HpyF3I CTNAG 4 cut(s) 369, 760, 999, 1197
HpySE526I ACGT 2 cut(s) 84, 849
Hsp92I GRCGYC 1 cut(s) 1047
Hsp92II CATG 4 cut(s) 388, 593, 955, 1111
HspAI GCGC 1 cut(s) 1105
KflI GGGWCCC 1 cut(s) 172
Kpn2I TCCGGA 2 cut(s) 478, 865
Ksp22I TGATCA 1 cut(s) 1072
KspAI GTTAAC 2 cut(s) 73, 264
Kzo9I GATC 5 cut(s) 475, 698, 862, 1056, 1072
LmnI GCTCC 2 cut(s) 481, 1132
Lsp1109I GCAGC 5 cut(s) 123, 457, 553, 852, 1246
LweI GCATC 3 cut(s) 162, 766, 1183
MaeI CTAG 2 cut(s) 467, 1394
MaeII ACGT 2 cut(s) 84, 849
MaeIII GTNAC 3 cut(s) 343, 755, 1033
MalI GATC 5 cut(s) 477, 700, 864, 1058, 1074
MboI GATC 5 cut(s) 475, 698, 862, 1056, 1072
MflI RGATCY 2 cut(s) 475, 1056
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 2 cut(s) 235, 487
MlyI GAGTC 3 cut(s) 752, 900, 1169
MmeI TCCRAC 1 cut(s) 816
Mph1103I ATGCAT 1 cut(s) 1138
MroI TCCGGA 2 cut(s) 478, 865
MseI TTAA 5 cut(s) 66, 72, 263, 813, 1158
MslI CAYNNNNRTG 3 cut(s) 566, 941, 1259
MspI CCGG 4 cut(s) 176, 479, 866, 1044
MspR9I CCNGG 7 cut(s) 150, 168, 176, 940, 986, 1246, 1279
Mva1269I GAATGC 2 cut(s) 543, 1236
MvaI CCWGG 6 cut(s) 150, 168, 940, 986, 1246, 1279
MwoI GCNNNNNNNGC 6 cut(s) 222, 470, 668, 837, 1085, 1133
NciI CCSGG 1 cut(s) 176
NcoI CCATGG 1 cut(s) 384
NdeII GATC 5 cut(s) 475, 698, 862, 1056, 1072
NlaIII CATG 4 cut(s) 388, 593, 955, 1111
NlaIV GGNNCC 4 cut(s) 173, 174, 477, 483
NmuCI GTSAC 3 cut(s) 343, 755, 1033
NsbI TGCGCA 1 cut(s) 1106
NsiI ATGCAT 1 cut(s) 1138
NspI RCATGY 2 cut(s) 955, 1111
OliI CACNNNNGTG 2 cut(s) 566, 1259
PasI CCCWGGG 1 cut(s) 167
PctI GAATGC 2 cut(s) 543, 1236
PfeI GAWTC 2 cut(s) 724, 1341
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 5 cut(s) 113, 472, 543, 842, 1236
PleI GAGTC 3 cut(s) 752, 899, 1169
PpsI GAGTC 3 cut(s) 752, 899, 1169
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 6 cut(s) 148, 166, 938, 984, 1244, 1277
PspGI CCWGG 6 cut(s) 148, 166, 938, 984, 1244, 1277
PspN4I GGNNCC 4 cut(s) 173, 174, 477, 483
PspPI GGNCC 3 cut(s) 33, 164, 172
PspPPI RGGWCCY 1 cut(s) 172
PstI CTGCAG 1 cut(s) 624
PsuI RGATCY 2 cut(s) 475, 1056
RsaI GTAC 1 cut(s) 1205
RsaNI GTAC 1 cut(s) 1204
RseI CAYNNNNRTG 3 cut(s) 566, 941, 1259
SaqAI TTAA 5 cut(s) 66, 72, 263, 813, 1158
SatI GCNGC 5 cut(s) 112, 471, 542, 841, 1235
Sau3AI GATC 5 cut(s) 475, 698, 862, 1056, 1072
Sau96I GGNCC 3 cut(s) 33, 164, 172
ScaI AGTACT 1 cut(s) 1205
SchI GAGTC 3 cut(s) 752, 900, 1169
ScrFI CCNGG 7 cut(s) 150, 168, 176, 940, 986, 1246, 1279
SduI GDGCHC 1 cut(s) 486
SfaNI GCATC 3 cut(s) 162, 766, 1183
SfcI CTRYAG 2 cut(s) 142, 620
SgrAI CRCCGGYG 1 cut(s) 1043
SinI GGWCC 2 cut(s) 33, 172
SmiMI CAYNNNNRTG 3 cut(s) 566, 941, 1259
SmlI CTYRAG 4 cut(s) 10, 16, 572, 686
SmoI CTYRAG 4 cut(s) 10, 16, 572, 686
SpeI ACTAGT 1 cut(s) 1393
Sse9I AATT 2 cut(s) 235, 487
SsiI CCGC 2 cut(s) 473, 564
SspI AATATT 1 cut(s) 809
SspMI CTAG 2 cut(s) 467, 1394
StyD4I CCNGG 7 cut(s) 148, 166, 174, 938, 984, 1244, 1277
StyI CCWWGG 4 cut(s) 384, 504, 1080, 1335
TaaI ACNGT 3 cut(s) 107, 449, 1408
TaiI ACGT 2 cut(s) 87, 852
TaqI TCGA 2 cut(s) 198, 897
TasI AATT 2 cut(s) 235, 487
TatI WGTACW 1 cut(s) 1203
TfiI GAWTC 2 cut(s) 724, 1341
Tru1I TTAA 5 cut(s) 66, 72, 263, 813, 1158
Tru9I TTAA 5 cut(s) 66, 72, 263, 813, 1158
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 3 cut(s) 343, 755, 1033
TseI GCWGC 5 cut(s) 111, 470, 541, 840, 1234
Tsp45I GTSAC 3 cut(s) 343, 755, 1033
TspDTI ATGAA 5 cut(s) 111, 224, 245, 644, 1343
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 1 cut(s) 454
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 2 cut(s) 33, 172
XagI CCTNNNNNAGG 1 cut(s) 1276
XapI RAATTY 1 cut(s) 235
XceI RCATGY 2 cut(s) 955, 1111
XcmI CCANNNNNNNNNTGG 1 cut(s) 799
XspI CTAG 2 cut(s) 467, 1394
ZrmI AGTACT 1 cut(s) 1205
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.