Rh3CG095900

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0008869
Physical Location & Seq
Reverse (-)
7528359 .. 7529995
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG095900.1

Sequence Viewer

Length: 1197 bp
ATGTTCGACAATTTGGGTACAGACGGCTACAGAGACTTGGCTATGGAGATTGGGGATCTATACGCTGACCACGGTGTTCTACTCCCGGTTGTGACCTTGACCGGCATCATTGATGAGTATGCCCAAGCCGGCAAAACTGCTGAGGCTCTCCATGTGTATCGGATAATGTTGGATTCTGAAGATTGTGCCCCCAATGCTTACACTTATAGTGTGATCATCAGGGCTCTGGCTGCAGACCCGGACCCCAATTTTCACGTTGTTGCCAAGGAGTTTGTGATGGAGATGATGGGGAAGGGATGGCAGCCGAATGCCAGCACATACACTGCTGTGTTTGAGGCGTTTGCAGGTCAGGAGAAGATGGAGGAGGCCAAGCAGTTTCTGGAGGAGATGAAAGCTAGTGGGTTTGTGCCTGACAAGAAAGCAATGAGGGCTTTCAGAAAGGTTCTAAAGGTTAAAGGGCCTGTTGTCAAAAGCATTTTCGATATTCTATTTGATAACTTTGATGACTATTCAGACGAGTCAGACGATGATGATGTTGTAAAGAAGTGCACCAAAGACTTTGACTGGTCGAAGTACCCCCCTGAGAGCTATGTTCCTTTCAGCAAGAAACAAGTCATCGAGGACGACCCAGAAAACCCCAAAGATTTGGAGCAAAAGTTCGACACAATGCACAGGAATGGAGCCCTCGTCAGCACTGTGACCAGGATGCTCAACATGCTAAAAAATGATGGATTCTTGTCCCAAGCCGAGGAGATCAGGACTGCGTTAGAAGTGGATCATAATCTGCCTGAAGTCGTGACTCATACTGGTGTCATTGAGATCTATGGCAGGCATGATGAGGCCAAGCATGCTCTCAAGGTGTTTTTGCGCATGTTATCCCTTGGAGTTTCCCCAAACGCATACACTTACAGTGTCCTCATTAAGGCACTCGCTAAAGACTCCAACTTTCTTGGTGATGCCAAGAAGTACTTACTGGAAATGATGGATAAGGGAATGCAGCCCAATGCCAGGACATACACCCCAGTATTCAAGGCCTTTGCCCGGCTAGAGCAGGATAATGTGAAGAAGGGGAAGGAGTTTCTTATGGAGATGGAAGCTATGGGGTTTAGGGCAAACAAGAAGGCTGTGATGGAGGTTCTCAAGGACAGAAAAGGGCCTGTTATTAAAACGGTCATCAAAATTCTCTTTGGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

398

Amino Acids

45.02

Weight (kDa)

5.46

Isoelectric Point (pI)

27.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 30 - 77 4e-09 PPR repeat family
PPR_long PF17177 38 - 141 4.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 102 - 139 1.1e-06 PPR repeat family
PPR_2 PF13041 263 - 312 1.8e-11 PPR repeat family
PPR_long PF17177 278 - 381 9.2e-10 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 869
Acc36I ACCTGC 1 cut(s) 335
AclWI GGATC 2 cut(s) 63, 783
AcsI RAATTY 1 cut(s) 1179
AcuI CTGAAG 2 cut(s) 198, 810
AfaI GTAC 3 cut(s) 19, 575, 968
AfiI CCNNNNNNNGG 4 cut(s) 748, 922, 1008, 1041
AgsI TTSAA 1 cut(s) 1030
AjnI CCWGG 2 cut(s) 701, 1007
AleI CACNNNNGTG 1 cut(s) 326
AloI GAACNNNNNNTCC 2 cut(s) 641, 673
AluBI AGCT 3 cut(s) 395, 588, 1097
AluI AGCT 3 cut(s) 395, 588, 1097
Alw21I GWGCWC 1 cut(s) 551
Alw26I GTCTC 1 cut(s) 27
Alw44I GTGCAC 1 cut(s) 547
AlwI GGATC 2 cut(s) 63, 783
AlwNI CAGNNNCTG 1 cut(s) 379
AoxI GGCC 5 cut(s) 366, 458, 840, 1032, 1154
ApaLI GTGCAC 1 cut(s) 547
ApeKI GCWGC 3 cut(s) 230, 301, 997
ApoI RAATTY 1 cut(s) 1179
AspLEI GCGC 1 cut(s) 870
AspS9I GGNCC 3 cut(s) 241, 458, 1154
AsuC2I CCSGG 3 cut(s) 86, 239, 1042
AsuHPI GGTGA 1 cut(s) 965
AvaII GGWCC 1 cut(s) 241
BaeGI GKGCMC 2 cut(s) 190, 551
BanII GRGCYC 2 cut(s) 226, 685
Bbv12I GWGCWC 1 cut(s) 551
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 217, 313, 1009
BccI CCATC 8 cut(s) 271, 280, 291, 352, 722, 976, 1084, 1123
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 2 cut(s) 703, 1009
BclI TGATCA 1 cut(s) 213
BcnI CCSGG 3 cut(s) 86, 239, 1042
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 2 cut(s) 396, 1046
BfmI CTRYAG 2 cut(s) 28, 231
BfuAI ACCTGC 1 cut(s) 335
BglII AGATCT 1 cut(s) 819
BisI GCNGC 3 cut(s) 231, 302, 998
BlsI GCNGC 3 cut(s) 232, 303, 999
BmcAI AGTACT 1 cut(s) 968
Bme1390I CCNGG 5 cut(s) 86, 239, 703, 1009, 1042
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 241, 458, 1154
BmiI GGNNCC 2 cut(s) 243, 682
BmrFI CCNGG 5 cut(s) 86, 239, 703, 1009, 1042
BmrI ACTGGG 1 cut(s) 1016
BmsI GCATC 3 cut(s) 114, 696, 946
BmuI ACTGGG 1 cut(s) 1016
BpmI CTGGAG 1 cut(s) 401
Bpu10I CCTNAGC 1 cut(s) 141
BpuEI CTTGAG 2 cut(s) 839, 1124
BpuMI CCSGG 3 cut(s) 86, 239, 1042
BsaBI GATNNNNATC 1 cut(s) 780
BsaJI CCNNGG 4 cut(s) 70, 264, 747, 880
BsaXI ACNNNNNCTCC 4 cut(s) 641, 671, 672, 702
Bsc4I CCNNNNNNNGG 4 cut(s) 748, 922, 1008, 1041
Bse118I RCCGGY 2 cut(s) 101, 128
Bse1I ACTGG 4 cut(s) 569, 811, 978, 1022
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 780
BseBI CCWGG 2 cut(s) 703, 1009
BseDI CCNNGG 4 cut(s) 70, 264, 747, 880
BseGI GGATG 2 cut(s) 302, 711
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 4 cut(s) 748, 922, 1008, 1041
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 2 cut(s) 132, 573
BseNI ACTGG 4 cut(s) 569, 811, 978, 1022
BseRI GAGGAG 3 cut(s) 377, 398, 764
BseSI GKGCMC 2 cut(s) 190, 551
BseXI GCAGC 3 cut(s) 217, 313, 1009
BshFI GGCC 5 cut(s) 368, 460, 842, 1034, 1156
BsiHKAI GWGCWC 1 cut(s) 551
BsiSI CCGG 5 cut(s) 86, 102, 129, 239, 1042
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 4 cut(s) 748, 922, 1008, 1041
BsmAI GTCTC 1 cut(s) 27
BsmFI GGGAC 1 cut(s) 724
BsmI GAATGC 2 cut(s) 313, 999
BsnI GGCC 5 cut(s) 368, 460, 842, 1034, 1156
Bsp1286I GDGCHC 4 cut(s) 190, 226, 551, 685
Bsp143I GATC 5 cut(s) 55, 213, 753, 775, 819
BspANI GGCC 5 cut(s) 368, 460, 842, 1034, 1156
BspCNI CTCAG 2 cut(s) 133, 574
BspLI GGNNCC 2 cut(s) 243, 682
BspMAI CTGCAG 1 cut(s) 235
BspMI ACCTGC 1 cut(s) 335
BspPI GGATC 2 cut(s) 63, 783
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 2 cut(s) 101, 128
BsrI ACTGG 4 cut(s) 569, 811, 978, 1022
BssAI RCCGGY 2 cut(s) 101, 128
BssECI CCNNGG 4 cut(s) 70, 264, 747, 880
BssMI GATC 5 cut(s) 55, 213, 753, 775, 819
BssT1I CCWWGG 2 cut(s) 264, 880
Bst2UI CCWGG 2 cut(s) 703, 1009
Bst4CI ACNGT 4 cut(s) 74, 697, 911, 1171
BstC8I GCNNGC 4 cut(s) 130, 313, 830, 849
BstDEI CTNAG 2 cut(s) 141, 582
BstDSI CCRYGG 1 cut(s) 70
BstENI CCTNNNNNAGG 1 cut(s) 920
BstF5I GGATG 2 cut(s) 302, 711
BstHHI GCGC 1 cut(s) 870
BstKTI GATC 5 cut(s) 58, 216, 756, 778, 822
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 5 cut(s) 55, 213, 753, 775, 819
BstMWI GCNNNNNNNGC 5 cut(s) 194, 230, 428, 715, 848
BstNI CCWGG 2 cut(s) 703, 1009
BstNSI RCATGY 3 cut(s) 718, 851, 874
BstSCI CCNGG 5 cut(s) 84, 237, 701, 1007, 1040
BstSFI CTRYAG 2 cut(s) 28, 231
BstSLI GKGCMC 2 cut(s) 190, 551
BstV1I GCAGC 3 cut(s) 217, 313, 1009
BstX2I RGATCY 2 cut(s) 55, 819
BstXI CCANNNNNNTGG 1 cut(s) 646
BstYI RGATCY 2 cut(s) 55, 819
BsuRI GGCC 5 cut(s) 368, 460, 842, 1034, 1156
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 2 cut(s) 302, 711
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 3 cut(s) 321, 693, 916
BveI ACCTGC 1 cut(s) 335
Cac8I GCNNGC 4 cut(s) 130, 313, 830, 849
CaiI CAGNNNCTG 1 cut(s) 379
CfoI GCGC 1 cut(s) 870
Cfr10I RCCGGY 2 cut(s) 101, 128
Cfr13I GGNCC 3 cut(s) 241, 458, 1154
Csp6I GTAC 3 cut(s) 18, 574, 967
CviAII CATG 5 cut(s) 152, 715, 833, 848, 871
CviQI GTAC 3 cut(s) 18, 574, 967
DdeI CTNAG 2 cut(s) 141, 582
DpnI GATC 5 cut(s) 57, 215, 755, 777, 821
DpnII GATC 5 cut(s) 55, 213, 753, 775, 819
Eco130I CCWWGG 2 cut(s) 264, 880
Eco147I AGGCCT 1 cut(s) 1034
Eco24I GRGCYC 2 cut(s) 226, 685
Eco47I GGWCC 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 198, 810
EcoNI CCTNNNNNAGG 1 cut(s) 920
EcoO109I RGGNCCY 2 cut(s) 458, 1154
EcoRII CCWGG 2 cut(s) 701, 1007
EcoT14I CCWWGG 2 cut(s) 264, 880
EcoT38I GRGCYC 2 cut(s) 226, 685
ErhI CCWWGG 2 cut(s) 264, 880
FaeI CATG 5 cut(s) 155, 718, 836, 851, 874
FalI AAGNNNNNCTT 2 cut(s) 953, 985
FaqI GGGAC 1 cut(s) 724
FatI CATG 5 cut(s) 151, 714, 832, 847, 870
FbaI TGATCA 1 cut(s) 213
Fnu4HI GCNGC 3 cut(s) 231, 302, 998
FokI GGATG 2 cut(s) 309, 718
FriOI GRGCYC 2 cut(s) 226, 685
Fsp4HI GCNGC 3 cut(s) 231, 302, 998
FspBI CTAG 2 cut(s) 396, 1046
FspI TGCGCA 1 cut(s) 869
GlaI GCGC 1 cut(s) 869
GluI GCNGC 3 cut(s) 231, 302, 998
GsuI CTGGAG 1 cut(s) 401
HaeIII GGCC 5 cut(s) 368, 460, 842, 1034, 1156
HapII CCGG 5 cut(s) 86, 102, 129, 239, 1042
HhaI GCGC 1 cut(s) 870
Hin1II CATG 5 cut(s) 155, 718, 836, 851, 874
Hin6I GCGC 1 cut(s) 868
HinP1I GCGC 1 cut(s) 868
HinfI GANTC 5 cut(s) 173, 518, 732, 799, 938
HpaII CCGG 5 cut(s) 86, 102, 129, 239, 1042
HphI GGTGA 1 cut(s) 965
Hpy166II GTNNAC 1 cut(s) 549
Hpy188I TCNGA 5 cut(s) 162, 178, 437, 514, 523
Hpy188III TCNNGA 4 cut(s) 350, 380, 757, 796
Hpy8I GTNNAC 1 cut(s) 549
HpyAV CCTTC 4 cut(s) 286, 1060, 1066, 1114
HpyCH4III ACNGT 4 cut(s) 74, 697, 911, 1171
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 5 cut(s) 233, 344, 549, 670, 997
HpyF10VI GCNNNNNNNGC 5 cut(s) 194, 230, 428, 715, 848
HpyF3I CTNAG 2 cut(s) 141, 582
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 5 cut(s) 155, 718, 836, 851, 874
HspAI GCGC 1 cut(s) 868
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 5 cut(s) 55, 213, 753, 775, 819
LmnI GCTCC 2 cut(s) 649, 680
Lsp1109I GCAGC 3 cut(s) 217, 313, 1009
LweI GCATC 3 cut(s) 114, 696, 946
MaeI CTAG 2 cut(s) 396, 1046
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 91, 697, 796
MalI GATC 5 cut(s) 57, 215, 755, 777, 821
MboI GATC 5 cut(s) 55, 213, 753, 775, 819
MboII GAAGA 3 cut(s) 191, 367, 1075
MflI RGATCY 2 cut(s) 55, 819
MhlI GDGCHC 4 cut(s) 190, 226, 551, 685
MluCI AATT 3 cut(s) 10, 247, 1179
MlyI GAGTC 3 cut(s) 527, 793, 932
MmeI TCCRAC 2 cut(s) 150, 966
MroNI GCCGGC 1 cut(s) 128
MseI TTAA 3 cut(s) 453, 921, 1164
MslI CAYNNNNRTG 3 cut(s) 326, 675, 807
MspI CCGG 5 cut(s) 86, 102, 129, 239, 1042
MspR9I CCNGG 5 cut(s) 86, 239, 703, 1009, 1042
Mva1269I GAATGC 2 cut(s) 313, 999
MvaI CCWGG 2 cut(s) 703, 1009
MwoI GCNNNNNNNGC 5 cut(s) 194, 230, 428, 715, 848
NaeI GCCGGC 1 cut(s) 130
NciI CCSGG 3 cut(s) 86, 239, 1042
NdeII GATC 5 cut(s) 55, 213, 753, 775, 819
NgoMIV GCCGGC 1 cut(s) 128
NlaIII CATG 5 cut(s) 155, 718, 836, 851, 874
NlaIV GGNNCC 2 cut(s) 243, 682
NmeAIII GCCGAG 1 cut(s) 772
NmuCI GTSAC 3 cut(s) 91, 697, 796
NsbI TGCGCA 1 cut(s) 869
NspI RCATGY 3 cut(s) 718, 851, 874
OliI CACNNNNGTG 1 cut(s) 326
PaeI GCATGC 1 cut(s) 851
PceI AGGCCT 1 cut(s) 1034
PcsI WCGNNNNNNNCGW 1 cut(s) 522
PctI GAATGC 2 cut(s) 313, 999
PdiI GCCGGC 1 cut(s) 130
PfeI GAWTC 2 cut(s) 173, 732
PkrI GCNGC 3 cut(s) 232, 303, 999
PleI GAGTC 3 cut(s) 526, 793, 932
PpsI GAGTC 3 cut(s) 526, 793, 932
Psp6I CCWGG 2 cut(s) 701, 1007
PspGI CCWGG 2 cut(s) 701, 1007
PspN4I GGNNCC 2 cut(s) 243, 682
PspPI GGNCC 3 cut(s) 241, 458, 1154
PstI CTGCAG 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 379
PsuI RGATCY 2 cut(s) 55, 819
RsaI GTAC 3 cut(s) 19, 575, 968
RsaNI GTAC 3 cut(s) 18, 574, 967
RseI CAYNNNNRTG 3 cut(s) 326, 675, 807
SaqAI TTAA 3 cut(s) 453, 921, 1164
SatI GCNGC 3 cut(s) 231, 302, 998
Sau3AI GATC 5 cut(s) 55, 213, 753, 775, 819
Sau96I GGNCC 3 cut(s) 241, 458, 1154
ScaI AGTACT 1 cut(s) 968
SchI GAGTC 3 cut(s) 527, 793, 932
ScrFI CCNGG 5 cut(s) 86, 239, 703, 1009, 1042
SduI GDGCHC 4 cut(s) 190, 226, 551, 685
SfaNI GCATC 3 cut(s) 114, 696, 946
SfcI CTRYAG 2 cut(s) 28, 231
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 3 cut(s) 326, 675, 807
SmlI CTYRAG 2 cut(s) 854, 1139
SmoI CTYRAG 2 cut(s) 854, 1139
SphI GCATGC 1 cut(s) 851
Sse9I AATT 3 cut(s) 10, 247, 1179
SseBI AGGCCT 1 cut(s) 1034
SspMI CTAG 2 cut(s) 396, 1046
StuI AGGCCT 1 cut(s) 1034
StyD4I CCNGG 5 cut(s) 84, 237, 701, 1007, 1040
StyI CCWWGG 2 cut(s) 264, 880
TaaI ACNGT 4 cut(s) 74, 697, 911, 1171
TaiI ACGT 1 cut(s) 258
TaqI TCGA 5 cut(s) 6, 480, 569, 618, 660
TasI AATT 3 cut(s) 10, 247, 1179
TatI WGTACW 1 cut(s) 966
TfiI GAWTC 2 cut(s) 173, 732
Tru1I TTAA 3 cut(s) 453, 921, 1164
Tru9I TTAA 3 cut(s) 453, 921, 1164
TscAI CASTG 3 cut(s) 328, 700, 916
TseFI GTSAC 3 cut(s) 91, 697, 796
TseI GCWGC 3 cut(s) 230, 301, 997
Tsp45I GTSAC 3 cut(s) 91, 697, 796
TspDTI ATGAA 1 cut(s) 404
TspRI CASTG 3 cut(s) 328, 700, 916
VneI GTGCAC 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 241
XagI CCTNNNNNAGG 1 cut(s) 920
XapI RAATTY 1 cut(s) 1179
XceI RCATGY 3 cut(s) 718, 851, 874
XcmI CCANNNNNNNNNTGG 1 cut(s) 376
XspI CTAG 2 cut(s) 396, 1046
ZrmI AGTACT 1 cut(s) 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.