FvH4_6g09510

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006132
Physical Location & Seq
Reverse (-)
5665690 .. 5669009
3320 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09510.t1

Sequence Viewer

Length: 774 bp
ATGATTAGAACAATGATGAAACGAACCCAGGAGCTGCTGGTGTTTCTGGTTCTGGCTTTGTTCTACAAGGCCACCGGCATGAAGCAGCCTTGCTGTAAGCCCCCGCAACCGGCCGACTGTGGCAGCTTTTGTTTGAGCTGGAGACTGGCTGTGGAGGCCAACAATGTGCGTGGGTGGCGCACTGTGCCAACACAGTGCTTTCTCTATCTCCAAACATACATGACCGGAGGACAGTACGACCGCGACGTCGAATTCATTGTTGATGAAATTACGAGTTATGTGAACGGAATCGTTCCCTCGGATGACGGCAAGGATGCATGGATTTTGGATGTGGACGACACTTGCATATCCAATGTCTATTACTACAAGGGAAAGAGATACGGGTGTGACCCTTATGATCCATCTGCGTTCAAAGCATGGGCGATGACAGGAGGGTGTCCGGCAATTCCTGCTATGTTGGGATTGTTTATCAAGCTGGTGAATAGTGGATTTAAGGTTATTTTGCTTACCGGAAGAGGTGAAGAAACACTAGGTCAAGTCACTATTGAGAATCTGCATAATCAAGGATTTCTTGGTTATGAACGGCTCATTTTGAGGACGGCAGCTTACAAAGGGCAGAGCGCGGTGGAGTACAAGTCGGCGATTAGGAAGAAACTGGTGGAAGAAGGTTACAGGATATGGGGGAATATAGGAGATCAATGGAGTGACCTGCATGGAGATTATGTAGGCAACCGTACGTTTAAGCTTCCTAATCCCATGTACTTTGTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

258

Amino Acids

29.09

Weight (kDa)

8.03

Isoelectric Point (pI)

28.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 41 - 256 8.4e-75 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
AatII GACGTC 1 cut(s) 249
Acc36I ACCTGC 1 cut(s) 717
AccII CGCG 2 cut(s) 243, 623
AciI CCGC 3 cut(s) 104, 241, 623
AclWI GGATC 1 cut(s) 392
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 1 cut(s) 251
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 4 cut(s) 236, 632, 736, 761
AfiI CCNNNNNNNGG 1 cut(s) 109
AgsI TTSAA 1 cut(s) 412
AjnI CCWGG 1 cut(s) 27
AluBI AGCT 6 cut(s) 34, 126, 138, 475, 605, 745
AluI AGCT 6 cut(s) 34, 126, 138, 475, 605, 745
Alw26I GTCTC 1 cut(s) 136
AlwI GGATC 1 cut(s) 392
AlwNI CAGNNNCTG 1 cut(s) 34
AoxI GGCC 3 cut(s) 69, 111, 156
ApeKI GCWGC 4 cut(s) 34, 85, 123, 602
ApoI RAATTY 1 cut(s) 251
AspLEI GCGC 2 cut(s) 180, 623
AsuHPI GGTGA 2 cut(s) 490, 530
BaeI ACNNNNGTAYC 2 cut(s) 370, 403
BbvI GCAGC 4 cut(s) 21, 97, 135, 614
BccI CCATC 1 cut(s) 409
BceAI ACGGC 3 cut(s) 322, 599, 615
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 136
BfaI CTAG 1 cut(s) 530
BfuAI ACCTGC 1 cut(s) 717
BisI GCNGC 4 cut(s) 35, 86, 124, 603
BlsI GCNGC 4 cut(s) 36, 87, 125, 604
Bme1390I CCNGG 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 1 cut(s) 304
BpmI CTGGAG 1 cut(s) 160
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 2 cut(s) 27, 297
BsaWI WCCGGW 2 cut(s) 224, 509
BsaXI ACNNNNNCTCC 4 cut(s) 620, 650, 708, 738
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse118I RCCGGY 2 cut(s) 74, 109
Bse1I ACTGG 2 cut(s) 150, 660
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 27, 297
BseGI GGATG 3 cut(s) 307, 319, 334
BseLI CCNNNNNNNGG 1 cut(s) 109
BseNI ACTGG 2 cut(s) 150, 660
BseX3I CGGCCG 1 cut(s) 111
BseXI GCAGC 4 cut(s) 21, 97, 135, 614
Bsh1236I CGCG 2 cut(s) 243, 623
Bsh1285I CGRYCG 2 cut(s) 114, 241
BshFI GGCC 3 cut(s) 71, 113, 158
BsiEI CGRYCG 2 cut(s) 114, 241
BsiSI CCGG 5 cut(s) 75, 110, 225, 440, 510
BsiWI CGTACG 1 cut(s) 734
BslI CCNNNNNNNGG 1 cut(s) 109
BsmAI GTCTC 1 cut(s) 136
BsnI GGCC 3 cut(s) 71, 113, 158
Bsp143I GATC 2 cut(s) 397, 694
BspACI CCGC 3 cut(s) 104, 241, 623
BspANI GGCC 3 cut(s) 71, 113, 158
BspFNI CGCG 2 cut(s) 243, 623
BspMI ACCTGC 1 cut(s) 717
BspPI GGATC 1 cut(s) 392
BsrFI RCCGGY 2 cut(s) 74, 109
BsrI ACTGG 2 cut(s) 150, 660
BssAI RCCGGY 2 cut(s) 74, 109
BssECI CCNNGG 2 cut(s) 27, 297
BssMI GATC 2 cut(s) 397, 694
BssNI GRCGYC 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 5 cut(s) 119, 184, 195, 234, 734
Bst6I CTCTTC 1 cut(s) 508
BstACI GRCGYC 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 449
BstF5I GGATG 3 cut(s) 307, 319, 334
BstFNI CGCG 2 cut(s) 243, 623
BstHHI GCGC 2 cut(s) 180, 623
BstKTI GATC 2 cut(s) 400, 697
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 2 cut(s) 397, 694
BstMCI CGRYCG 2 cut(s) 114, 241
BstMWI GCNNNNNNNGC 5 cut(s) 155, 175, 184, 413, 449
BstNI CCWGG 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 27
BstUI CGCG 2 cut(s) 243, 623
BstV1I GCAGC 4 cut(s) 21, 97, 135, 614
BstZI CGGCCG 1 cut(s) 111
BsuRI GGCC 3 cut(s) 71, 113, 158
BtgZI GCGATG 1 cut(s) 437
BtsCI GGATG 3 cut(s) 307, 319, 334
BtsIMutI CAGTG 2 cut(s) 180, 200
BveI ACCTGC 1 cut(s) 717
CaiI CAGNNNCTG 1 cut(s) 34
CfoI GCGC 2 cut(s) 180, 623
Cfr10I RCCGGY 2 cut(s) 74, 109
Csp6I GTAC 4 cut(s) 235, 631, 735, 760
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 6 cut(s) 79, 220, 318, 417, 713, 757
CviQI GTAC 4 cut(s) 235, 631, 735, 760
DpnI GATC 2 cut(s) 399, 696
DpnII GATC 2 cut(s) 397, 694
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 111
EagI CGGCCG 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 508
EclXI CGGCCG 1 cut(s) 111
Eco52I CGGCCG 1 cut(s) 111
EcoRI GAATTC 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 27
EcoT22I ATGCAT 1 cut(s) 319
FaeI CATG 6 cut(s) 82, 223, 321, 420, 716, 760
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FatI CATG 6 cut(s) 78, 219, 317, 416, 712, 756
FauI CCCGC 1 cut(s) 111
Fnu4HI GCNGC 4 cut(s) 35, 86, 124, 603
FokI GGATG 3 cut(s) 314, 326, 341
Fsp4HI GCNGC 4 cut(s) 35, 86, 124, 603
FspBI CTAG 1 cut(s) 530
GlaI GCGC 2 cut(s) 179, 622
GluI GCNGC 4 cut(s) 35, 86, 124, 603
GsuI CTGGAG 1 cut(s) 160
HaeIII GGCC 3 cut(s) 71, 113, 158
HapII CCGG 5 cut(s) 75, 110, 225, 440, 510
HhaI GCGC 2 cut(s) 180, 623
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 6 cut(s) 82, 223, 321, 420, 716, 760
Hin6I GCGC 2 cut(s) 178, 621
HinP1I GCGC 2 cut(s) 178, 621
HindIII AAGCTT 1 cut(s) 743
HinfI GANTC 2 cut(s) 288, 550
HpaII CCGG 5 cut(s) 75, 110, 225, 440, 510
HphI GGTGA 2 cut(s) 490, 530
Hpy166II GTNNAC 2 cut(s) 283, 334
Hpy188I TCNGA 1 cut(s) 301
Hpy8I GTNNAC 2 cut(s) 283, 334
Hpy99I CGWCG 2 cut(s) 248, 251
HpyAV CCTTC 1 cut(s) 659
HpyCH4III ACNGT 5 cut(s) 119, 184, 195, 234, 734
HpyCH4IV ACGT 2 cut(s) 246, 737
HpyCH4V TGCA 4 cut(s) 317, 345, 556, 712
HpyF10VI GCNNNNNNNGC 5 cut(s) 155, 175, 184, 413, 449
HpySE526I ACGT 2 cut(s) 246, 737
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 6 cut(s) 82, 223, 321, 420, 716, 760
HspAI GCGC 2 cut(s) 178, 621
Kzo9I GATC 2 cut(s) 397, 694
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 4 cut(s) 21, 97, 135, 614
LweI GCATC 1 cut(s) 304
MaeI CTAG 1 cut(s) 530
MaeII ACGT 2 cut(s) 246, 737
MaeIII GTNAC 4 cut(s) 386, 538, 668, 704
MalI GATC 2 cut(s) 399, 696
MboI GATC 2 cut(s) 397, 694
MboII GAAGA 4 cut(s) 525, 533, 661, 674
MluCI AATT 3 cut(s) 251, 267, 444
MnlI CCTC 6 cut(s) 148, 221, 307, 425, 509, 588
Mph1103I ATGCAT 1 cut(s) 319
MseI TTAA 3 cut(s) 492, 741, 772
MslI CAYNNNNRTG 1 cut(s) 77
MspI CCGG 5 cut(s) 75, 110, 225, 440, 510
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 2 cut(s) 243, 623
MwoI GCNNNNNNNGC 5 cut(s) 155, 175, 184, 413, 449
NdeII GATC 2 cut(s) 397, 694
NlaIII CATG 6 cut(s) 82, 223, 321, 420, 716, 760
NmuCI GTSAC 3 cut(s) 386, 538, 704
NsiI ATGCAT 1 cut(s) 319
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PfeI GAWTC 2 cut(s) 288, 550
Pfl23II CGTACG 1 cut(s) 734
PkrI GCNGC 4 cut(s) 36, 87, 125, 604
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspLI CGTACG 1 cut(s) 734
PstNI CAGNNNCTG 1 cut(s) 34
RsaI GTAC 4 cut(s) 236, 632, 736, 761
RsaNI GTAC 4 cut(s) 235, 631, 735, 760
RseI CAYNNNNRTG 1 cut(s) 77
SaqAI TTAA 3 cut(s) 492, 741, 772
SatI GCNGC 4 cut(s) 35, 86, 124, 603
Sau3AI GATC 2 cut(s) 397, 694
ScrFI CCNGG 1 cut(s) 29
SfaNI GCATC 1 cut(s) 304
SmiMI CAYNNNNRTG 1 cut(s) 77
Sse9I AATT 3 cut(s) 251, 267, 444
SsiI CCGC 3 cut(s) 104, 241, 623
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 1 cut(s) 27
TaaI ACNGT 5 cut(s) 119, 184, 195, 234, 734
TaiI ACGT 2 cut(s) 249, 740
TaqI TCGA 1 cut(s) 249
TasI AATT 3 cut(s) 251, 267, 444
TatI WGTACW 2 cut(s) 630, 759
TfiI GAWTC 2 cut(s) 288, 550
Tru1I TTAA 3 cut(s) 492, 741, 772
Tru9I TTAA 3 cut(s) 492, 741, 772
TscAI CASTG 2 cut(s) 187, 200
TseFI GTSAC 3 cut(s) 386, 538, 704
TseI GCWGC 4 cut(s) 34, 85, 123, 602
Tsp45I GTSAC 3 cut(s) 386, 538, 704
TspDTI ATGAA 5 cut(s) 32, 95, 244, 279, 594
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 2 cut(s) 187, 200
XapI RAATTY 1 cut(s) 251
XspI CTAG 1 cut(s) 530
ZraI GACGTC 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.