MD12G1192100.v1.1

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006132
Physical Location & Seq
Forward (+)
27402591 .. 27406811
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1192100.v1.1.491

Sequence Viewer

Length: 759 bp
ATGATGCAACGGACCTACGAGGCGTTTTTACTTCTGTTTCTGGCCTTACTCTCCAAGGCAACTAGTGTCAAGCCTTGCCCTAGGCCAAGCCTGCCGCAGGACGACAGCAGCTTTTGTTTGAGTTGGAGACTGGCGGTGGAGGCCAACAATCTGCGTGGGTGGCGCACGGTGCCAACTCAGTGCTTCCGCTACCTCGAAACTTACATGATTGGAGGTCAGTATGAACGGGACATCGATTTCATCCTTGGCCAAATCCTTAGCTATGCGAATGGAATAGCTTTATCTGATGATGGCATGGATGCTTGGATTTTGGACGTGGATGACACTTGCTTATCTAATCTCTTCTACTACAAGGGCAAGCGATACGGGTGCGATCCTTATGATCCATCCGGGTTCAAGGCATGGGCAATGACTGGAGGGTGCCCAGCAGTTCCTGGTATGCTGGGACTTTTTAGCAAGCTGGTGAATAGTGGGTTTAAGGTGATCATGCTCACAGGAAGAGATGAAGAAACCCTAGGCCAAATTACTGCCGCAAACTTGCATAACCAGGGATTTGTTGGTTACGAACGGCTAATTTTGAGGACTCCAGCTTACAAGGGGCAGAGCGCAGTTGTGTACAAATCAAACATTCGGAAGCAATTGGCAGAAGAAGGTTACAGAATTTGGGGAAATATAGGAGACCAGTGGACTGATCTTCAAGGAGACTATGTTGGCAACCGCACTTATAAGCTTCCAAACCCCATGTACTTTGTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

253

Amino Acids

28.45

Weight (kDa)

6.59

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 35 - 251 2.6e-73 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AccB1I GGYRCC 2 cut(s) 169, 420
AciI CCGC 5 cut(s) 95, 134, 187, 531, 718
AclWI GGATC 2 cut(s) 368, 377
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 1 cut(s) 660
AfaI GTAC 2 cut(s) 617, 746
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 2 cut(s) 397, 698
AhlI ACTAGT 1 cut(s) 62
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 2 cut(s) 433, 546
AluBI AGCT 6 cut(s) 111, 261, 278, 460, 590, 730
AluI AGCT 6 cut(s) 111, 261, 278, 460, 590, 730
Alw26I GTCTC 3 cut(s) 121, 672, 696
AlwI GGATC 2 cut(s) 368, 377
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 5 cut(s) 42, 83, 141, 247, 517
ApeKI GCWGC 1 cut(s) 108
ApoI RAATTY 1 cut(s) 660
AspA2I CCTAGG 2 cut(s) 80, 514
AspLEI GCGC 2 cut(s) 165, 608
AspS9I GGNCC 1 cut(s) 12
AsuC2I CCSGG 1 cut(s) 391
AsuHPI GGTGA 2 cut(s) 475, 493
AvaII GGWCC 1 cut(s) 12
AvrII CCTAGG 2 cut(s) 80, 514
BaeGI GKGCMC 1 cut(s) 425
BalI TGGCCA 1 cut(s) 249
BanI GGYRCC 2 cut(s) 169, 420
BbvI GCAGC 1 cut(s) 120
BccI CCATC 2 cut(s) 284, 394
BceAI ACGGC 1 cut(s) 584
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BciT130I CCWGG 2 cut(s) 435, 548
BclI TGATCA 1 cut(s) 483
BcnI CCSGG 1 cut(s) 391
BcoDI GTCTC 3 cut(s) 121, 672, 696
BcuI ACTAGT 1 cut(s) 62
BfaI CTAG 3 cut(s) 63, 81, 515
BisI GCNGC 3 cut(s) 95, 109, 531
BlnI CCTAGG 2 cut(s) 80, 514
BlsI GCNGC 3 cut(s) 96, 110, 532
Bme1390I CCNGG 3 cut(s) 391, 435, 548
Bme18I GGWCC 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 2 cut(s) 171, 422
BmrFI CCNGG 3 cut(s) 391, 435, 548
BmsI GCATC 1 cut(s) 289
BpmI CTGGAG 2 cut(s) 435, 570
Bpu10I CCTNAGC 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 391
Bsa29I ATCGAT 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 672
BsaJI CCNNGG 5 cut(s) 54, 80, 244, 514, 547
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 3 cut(s) 135, 418, 682
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 2 cut(s) 435, 548
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 5 cut(s) 54, 80, 244, 514, 547
BseGI GGATG 4 cut(s) 240, 304, 325, 386
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 3 cut(s) 135, 418, 682
BseSI GKGCMC 1 cut(s) 425
BseXI GCAGC 1 cut(s) 120
BseYI CCCAGC 2 cut(s) 424, 442
BshFI GGCC 5 cut(s) 44, 85, 143, 249, 519
BshNI GGYRCC 2 cut(s) 169, 420
BshVI ATCGAT 1 cut(s) 234
BsiSI CCGG 1 cut(s) 390
BslFI GGGAC 2 cut(s) 242, 459
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 3 cut(s) 121, 672, 696
BsmFI GGGAC 2 cut(s) 242, 459
BsnI GGCC 5 cut(s) 44, 85, 143, 249, 519
Bso31I GGTCTC 1 cut(s) 672
Bsp1286I GDGCHC 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 4 cut(s) 373, 382, 483, 691
BspACI CCGC 5 cut(s) 95, 134, 187, 531, 718
BspANI GGCC 5 cut(s) 44, 85, 143, 249, 519
BspCNI CTCAG 1 cut(s) 190
BspDI ATCGAT 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 171, 422
BspPI GGATC 2 cut(s) 368, 377
BspT107I GGYRCC 2 cut(s) 169, 420
BspTNI GGTCTC 1 cut(s) 672
BsrDI GCAATG 1 cut(s) 414
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 3 cut(s) 135, 418, 682
BssECI CCNNGG 5 cut(s) 54, 80, 244, 514, 547
BssMI GATC 4 cut(s) 373, 382, 483, 691
BssT1I CCWWGG 4 cut(s) 54, 80, 244, 514
Bst2UI CCWGG 2 cut(s) 435, 548
Bst4CI ACNGT 1 cut(s) 169
Bst6I CTCTTC 2 cut(s) 347, 493
BstAUI TGTACA 1 cut(s) 615
BstC8I GCNNGC 3 cut(s) 92, 359, 458
BstDEI CTNAG 2 cut(s) 177, 257
BstENI CCTNNNNNAGG 1 cut(s) 95
BstF5I GGATG 4 cut(s) 240, 304, 325, 386
BstHHI GCGC 2 cut(s) 165, 608
BstKTI GATC 4 cut(s) 376, 385, 486, 694
BstMAI GTCTC 3 cut(s) 121, 672, 696
BstMBI GATC 4 cut(s) 373, 382, 483, 691
BstMWI GCNNNNNNNGC 4 cut(s) 91, 140, 160, 169
BstNI CCWGG 2 cut(s) 435, 548
BstSCI CCNGG 3 cut(s) 389, 433, 546
BstSLI GKGCMC 1 cut(s) 425
BstV1I GCAGC 1 cut(s) 120
Bsu15I ATCGAT 1 cut(s) 234
BsuRI GGCC 5 cut(s) 44, 85, 143, 249, 519
BsuTUI ATCGAT 1 cut(s) 234
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 4 cut(s) 240, 304, 325, 386
BtsIMutI CAGTG 2 cut(s) 185, 689
Cac8I GCNNGC 3 cut(s) 92, 359, 458
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 2 cut(s) 165, 608
Cfr13I GGNCC 1 cut(s) 12
ClaI ATCGAT 1 cut(s) 234
Csp6I GTAC 2 cut(s) 616, 745
CspCI CAANNNNNGTGG 2 cut(s) 136, 171
CviAII CATG 5 cut(s) 205, 295, 402, 487, 742
CviQI GTAC 2 cut(s) 616, 745
DdeI CTNAG 2 cut(s) 177, 257
DpnI GATC 4 cut(s) 375, 384, 485, 693
DpnII GATC 4 cut(s) 373, 382, 483, 691
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 2 cut(s) 347, 493
EarI CTCTTC 2 cut(s) 347, 493
Eco130I CCWWGG 4 cut(s) 54, 80, 244, 514
Eco31I GGTCTC 1 cut(s) 672
Eco47I GGWCC 1 cut(s) 12
EcoNI CCTNNNNNAGG 1 cut(s) 95
EcoRII CCWGG 2 cut(s) 433, 546
EcoT14I CCWWGG 4 cut(s) 54, 80, 244, 514
ErhI CCWWGG 4 cut(s) 54, 80, 244, 514
FaeI CATG 5 cut(s) 208, 298, 405, 490, 745
FaqI GGGAC 2 cut(s) 242, 459
FatI CATG 5 cut(s) 204, 294, 401, 486, 741
FbaI TGATCA 1 cut(s) 483
Fnu4HI GCNGC 3 cut(s) 95, 109, 531
FokI GGATG 4 cut(s) 227, 311, 332, 373
Fsp4HI GCNGC 3 cut(s) 95, 109, 531
FspBI CTAG 3 cut(s) 63, 81, 515
GlaI GCGC 2 cut(s) 164, 607
GluI GCNGC 3 cut(s) 95, 109, 531
GsaI CCCAGC 2 cut(s) 428, 446
GsuI CTGGAG 2 cut(s) 435, 570
HaeIII GGCC 5 cut(s) 44, 85, 143, 249, 519
HapII CCGG 1 cut(s) 390
HhaI GCGC 2 cut(s) 165, 608
Hin1II CATG 5 cut(s) 208, 298, 405, 490, 745
Hin6I GCGC 2 cut(s) 163, 606
HinP1I GCGC 2 cut(s) 163, 606
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 1 cut(s) 583
HpaII CCGG 1 cut(s) 390
HphI GGTGA 2 cut(s) 475, 493
Hpy166II GTNNAC 2 cut(s) 616, 687
Hpy188I TCNGA 2 cut(s) 286, 633
Hpy8I GTNNAC 2 cut(s) 616, 687
HpyAV CCTTC 1 cut(s) 644
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 2 cut(s) 7, 541
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 140, 160, 169
HpyF3I CTNAG 2 cut(s) 177, 257
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 5 cut(s) 208, 298, 405, 490, 745
HspAI GCGC 2 cut(s) 163, 606
Ksp22I TGATCA 1 cut(s) 483
Kzo9I GATC 4 cut(s) 373, 382, 483, 691
Lsp1109I GCAGC 1 cut(s) 120
LweI GCATC 1 cut(s) 289
MaeI CTAG 3 cut(s) 63, 81, 515
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 2 cut(s) 560, 653
MalI GATC 4 cut(s) 375, 384, 485, 693
MboI GATC 4 cut(s) 373, 382, 483, 691
MboII GAAGA 5 cut(s) 334, 510, 518, 659, 686
MfeI CAATTG 1 cut(s) 638
MhlI GDGCHC 1 cut(s) 425
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 4 cut(s) 522, 573, 638, 660
MluNI TGGCCA 1 cut(s) 249
MlyI GAGTC 1 cut(s) 577
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 6 cut(s) 13, 133, 203, 206, 410, 573
Mox20I TGGCCA 1 cut(s) 249
MscI TGGCCA 1 cut(s) 249
MseI TTAA 2 cut(s) 477, 757
Msp20I TGGCCA 1 cut(s) 249
MspI CCGG 1 cut(s) 390
MspR9I CCNGG 3 cut(s) 391, 435, 548
MunI CAATTG 1 cut(s) 638
MvaI CCWGG 2 cut(s) 435, 548
MwoI GCNNNNNNNGC 4 cut(s) 91, 140, 160, 169
NciI CCSGG 1 cut(s) 391
NdeII GATC 4 cut(s) 373, 382, 483, 691
NlaIII CATG 5 cut(s) 208, 298, 405, 490, 745
NlaIV GGNNCC 2 cut(s) 171, 422
PkrI GCNGC 3 cut(s) 96, 110, 532
PleI GAGTC 1 cut(s) 577
PpsI GAGTC 1 cut(s) 577
PsiI TTATAA 1 cut(s) 726
Psp6I CCWGG 2 cut(s) 433, 546
PspFI CCCAGC 2 cut(s) 424, 442
PspGI CCWGG 2 cut(s) 433, 546
PspN4I GGNNCC 2 cut(s) 171, 422
PspPI GGNCC 1 cut(s) 12
PstNI CAGNNNCTG 1 cut(s) 434
RsaI GTAC 2 cut(s) 617, 746
RsaNI GTAC 2 cut(s) 616, 745
SaqAI TTAA 2 cut(s) 477, 757
SatI GCNGC 3 cut(s) 95, 109, 531
Sau3AI GATC 4 cut(s) 373, 382, 483, 691
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 1 cut(s) 577
ScrFI CCNGG 3 cut(s) 391, 435, 548
SduI GDGCHC 1 cut(s) 425
SfaNI GCATC 1 cut(s) 289
SinI GGWCC 1 cut(s) 12
SpeI ACTAGT 1 cut(s) 62
Sse9I AATT 4 cut(s) 522, 573, 638, 660
SsiI CCGC 5 cut(s) 95, 134, 187, 531, 718
SspMI CTAG 3 cut(s) 63, 81, 515
StyD4I CCNGG 3 cut(s) 389, 433, 546
StyI CCWWGG 4 cut(s) 54, 80, 244, 514
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 1 cut(s) 318
TaqI TCGA 2 cut(s) 195, 234
TasI AATT 4 cut(s) 522, 573, 638, 660
TatI WGTACW 2 cut(s) 615, 744
TauI GCSGC 2 cut(s) 97, 533
Tru1I TTAA 2 cut(s) 477, 757
Tru9I TTAA 2 cut(s) 477, 757
TscAI CASTG 2 cut(s) 185, 689
TseI GCWGC 1 cut(s) 108
TspDTI ATGAA 3 cut(s) 229, 237, 519
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 2 cut(s) 185, 689
VpaK11BI GGWCC 1 cut(s) 12
XagI CCTNNNNNAGG 1 cut(s) 95
XapI RAATTY 1 cut(s) 660
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XmaJI CCTAGG 2 cut(s) 80, 514
XspI CTAG 3 cut(s) 63, 81, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.