MD04G1176900.v1.1

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006132
Physical Location & Seq
Forward (+)
26830626 .. 26833890
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1176900.v1.1.491

Sequence Viewer

Length: 759 bp
ATGATGCAGCGGACCCGCGAGGCGTTTGTTTTTCTGTTTCTGGCCTTACTCTCCAAGGCAACTAGTGTGAAGCCTTGCCCCAGGCCAAGCCTGCCGCAGTCCGACAGCAACTTCTGTTTGAGTTGGAGACTGGCGGTGGAGACCAACAATATGCGTCGGTGGCGCACGGTGCCAATTCAGTGCTTTCGCTACGTCGAAACTTACATGATCGGAGGTCAGTATGAACGGGACATCAATTTTATCGTTGGCCAAATTCTTAGCTATGCGATTGGAATAGCTTTATCTAATGATGGCATGGATGCTTGGATTTTGGACGTGGATGACACTTGCTTATCTAATCTCTTCTACTACAAGAGCAGGCGATACGGGTGCGATCCTTTTGATTCATCTGGGTTCAAGGCATGGGCAAGGACTGGAGGGTGCCCAGCAGTTCCTGGTATGCTGGGACTTTTTAGCAAGCTGGTGAATAGTGGGTTTAAGGTGTTCATGGTCACAGGAAGAGATGAAAAGACCCTAGGCCAAATTACTACCGCAAACTTGCATGATCAGGGATTTGTTGGTTATGAACGGCTAATTTTGAGGACTCCAGTTTACAAAGGGCAGAGCGCAGTTGTGTACAAATCAAACGTTCGGAAGCAATTGGTAGAACAAGGTTACAGAATTTGGGGAAATATAGGAGACCAGTGGACTGATCTTCAAGGAGATTGTGTTGGCAACCGCACTTTTAAGCTTCCAAATCCCATGTACTTTGTCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

253

Amino Acids

28.67

Weight (kDa)

9.15

Isoelectric Point (pI)

33.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 36 - 251 5.7e-75 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 169, 420
AccII CGCG 1 cut(s) 18
AciI CCGC 6 cut(s) 10, 16, 95, 134, 531, 718
AclI AACGTT 1 cut(s) 627
AclWI GGATC 1 cut(s) 368
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 2 cut(s) 252, 660
AfaI GTAC 2 cut(s) 617, 746
AgsI TTSAA 2 cut(s) 397, 698
AhlI ACTAGT 1 cut(s) 62
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 2 cut(s) 80, 433
AluBI AGCT 4 cut(s) 261, 278, 460, 730
AluI AGCT 4 cut(s) 261, 278, 460, 730
Alw26I GTCTC 3 cut(s) 121, 134, 672
AlwI GGATC 1 cut(s) 368
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 4 cut(s) 42, 83, 247, 517
ApeKI GCWGC 1 cut(s) 7
ApoI RAATTY 2 cut(s) 252, 660
AspA2I CCTAGG 1 cut(s) 514
AspLEI GCGC 2 cut(s) 165, 608
AspS9I GGNCC 1 cut(s) 12
AsuHPI GGTGA 1 cut(s) 475
AvaII GGWCC 1 cut(s) 12
AvrII CCTAGG 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 425
BalI TGGCCA 1 cut(s) 249
BanI GGYRCC 2 cut(s) 169, 420
BbvI GCAGC 1 cut(s) 19
BccI CCATC 1 cut(s) 284
BceAI ACGGC 1 cut(s) 584
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BciT130I CCWGG 2 cut(s) 82, 435
BclI TGATCA 1 cut(s) 544
BcoDI GTCTC 3 cut(s) 121, 134, 672
BcuI ACTAGT 1 cut(s) 62
BfaI CTAG 2 cut(s) 63, 515
BisI GCNGC 2 cut(s) 8, 95
BlnI CCTAGG 1 cut(s) 514
BlsI GCNGC 2 cut(s) 9, 96
Bme1390I CCNGG 2 cut(s) 82, 435
Bme18I GGWCC 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 3 cut(s) 14, 171, 422
BmrFI CCNGG 2 cut(s) 82, 435
BmsI GCATC 1 cut(s) 289
BpmI CTGGAG 2 cut(s) 435, 570
BsaI GGTCTC 2 cut(s) 134, 672
BsaJI CCNNGG 3 cut(s) 54, 80, 514
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bse1I ACTGG 4 cut(s) 135, 418, 587, 682
BseBI CCWGG 2 cut(s) 82, 435
BseDI CCNNGG 3 cut(s) 54, 80, 514
BseGI GGATG 2 cut(s) 304, 325
BseNI ACTGG 4 cut(s) 135, 418, 587, 682
BseSI GKGCMC 1 cut(s) 425
BseXI GCAGC 1 cut(s) 19
BseYI CCCAGC 2 cut(s) 424, 442
Bsh1236I CGCG 1 cut(s) 18
BshFI GGCC 4 cut(s) 44, 85, 249, 519
BshNI GGYRCC 2 cut(s) 169, 420
BslFI GGGAC 3 cut(s) 242, 459, 737
BsmAI GTCTC 3 cut(s) 121, 134, 672
BsmFI GGGAC 3 cut(s) 242, 459, 737
BsnI GGCC 4 cut(s) 44, 85, 249, 519
Bso31I GGTCTC 2 cut(s) 134, 672
Bsp1286I GDGCHC 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 4 cut(s) 207, 373, 544, 691
BspACI CCGC 6 cut(s) 10, 16, 95, 134, 531, 718
BspANI GGCC 4 cut(s) 44, 85, 249, 519
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 3 cut(s) 14, 171, 422
BspPI GGATC 1 cut(s) 368
BspT107I GGYRCC 2 cut(s) 169, 420
BspTNI GGTCTC 2 cut(s) 134, 672
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 4 cut(s) 135, 418, 587, 682
BssECI CCNNGG 3 cut(s) 54, 80, 514
BssMI GATC 4 cut(s) 207, 373, 544, 691
BssT1I CCWWGG 2 cut(s) 54, 514
Bst2UI CCWGG 2 cut(s) 82, 435
Bst4CI ACNGT 1 cut(s) 169
Bst6I CTCTTC 2 cut(s) 347, 493
BstAUI TGTACA 1 cut(s) 615
BstC8I GCNNGC 3 cut(s) 92, 359, 458
BstDEI CTNAG 1 cut(s) 257
BstF5I GGATG 2 cut(s) 304, 325
BstFNI CGCG 1 cut(s) 18
BstHHI GCGC 2 cut(s) 165, 608
BstKTI GATC 4 cut(s) 210, 376, 547, 694
BstMAI GTCTC 3 cut(s) 121, 134, 672
BstMBI GATC 4 cut(s) 207, 373, 544, 691
BstMWI GCNNNNNNNGC 3 cut(s) 91, 160, 169
BstNI CCWGG 2 cut(s) 82, 435
BstSCI CCNGG 2 cut(s) 80, 433
BstSLI GKGCMC 1 cut(s) 425
BstUI CGCG 1 cut(s) 18
BstV1I GCAGC 1 cut(s) 19
BsuRI GGCC 4 cut(s) 44, 85, 249, 519
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 2 cut(s) 304, 325
BtsIMutI CAGTG 2 cut(s) 185, 689
Cac8I GCNNGC 3 cut(s) 92, 359, 458
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 2 cut(s) 165, 608
Cfr13I GGNCC 1 cut(s) 12
CseI GACGC 1 cut(s) 143
Csp6I GTAC 2 cut(s) 616, 745
CviAII CATG 6 cut(s) 205, 295, 402, 487, 542, 742
CviQI GTAC 2 cut(s) 616, 745
DdeI CTNAG 1 cut(s) 257
DpnI GATC 4 cut(s) 209, 375, 546, 693
DpnII GATC 4 cut(s) 207, 373, 544, 691
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 2 cut(s) 347, 493
EarI CTCTTC 2 cut(s) 347, 493
Eco130I CCWWGG 2 cut(s) 54, 514
Eco31I GGTCTC 2 cut(s) 134, 672
Eco47I GGWCC 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 80, 433
EcoT14I CCWWGG 2 cut(s) 54, 514
ErhI CCWWGG 2 cut(s) 54, 514
FaeI CATG 6 cut(s) 208, 298, 405, 490, 545, 745
FaqI GGGAC 3 cut(s) 242, 459, 737
FatI CATG 6 cut(s) 204, 294, 401, 486, 541, 741
FauI CCCGC 1 cut(s) 23
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 8, 95
FokI GGATG 2 cut(s) 311, 332
Fsp4HI GCNGC 2 cut(s) 8, 95
FspBI CTAG 2 cut(s) 63, 515
GlaI GCGC 2 cut(s) 164, 607
GluI GCNGC 2 cut(s) 8, 95
GsaI CCCAGC 2 cut(s) 428, 446
GsuI CTGGAG 2 cut(s) 435, 570
HaeIII GGCC 4 cut(s) 44, 85, 249, 519
HgaI GACGC 1 cut(s) 143
HhaI GCGC 2 cut(s) 165, 608
Hin1II CATG 6 cut(s) 208, 298, 405, 490, 545, 745
Hin6I GCGC 2 cut(s) 163, 606
HinP1I GCGC 2 cut(s) 163, 606
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 2 cut(s) 383, 583
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 3 cut(s) 592, 616, 687
Hpy188I TCNGA 3 cut(s) 103, 212, 633
Hpy8I GTNNAC 3 cut(s) 592, 616, 687
Hpy99I CGWCG 2 cut(s) 159, 197
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 3 cut(s) 192, 315, 627
HpyCH4V TGCA 2 cut(s) 7, 541
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 160, 169
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 3 cut(s) 192, 315, 627
Hsp92II CATG 6 cut(s) 208, 298, 405, 490, 545, 745
HspAI GCGC 2 cut(s) 163, 606
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 4 cut(s) 207, 373, 544, 691
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 1 cut(s) 289
MaeI CTAG 2 cut(s) 63, 515
MaeII ACGT 3 cut(s) 192, 315, 627
MaeIII GTNAC 2 cut(s) 490, 653
MalI GATC 4 cut(s) 209, 375, 546, 693
MboI GATC 4 cut(s) 207, 373, 544, 691
MboII GAAGA 3 cut(s) 334, 510, 686
MfeI CAATTG 1 cut(s) 638
MhlI GDGCHC 1 cut(s) 425
MlsI TGGCCA 1 cut(s) 249
MluCI AATT 7 cut(s) 174, 235, 252, 522, 573, 638, 660
MluNI TGGCCA 1 cut(s) 249
MlyI GAGTC 1 cut(s) 577
MmeI TCCRAC 2 cut(s) 104, 126
MnlI CCTC 4 cut(s) 13, 206, 410, 573
Mox20I TGGCCA 1 cut(s) 249
MscI TGGCCA 1 cut(s) 249
MseI TTAA 3 cut(s) 477, 726, 757
Msp20I TGGCCA 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 10
MspR9I CCNGG 2 cut(s) 82, 435
MunI CAATTG 1 cut(s) 638
MvaI CCWGG 2 cut(s) 82, 435
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 3 cut(s) 91, 160, 169
NdeII GATC 4 cut(s) 207, 373, 544, 691
NlaIII CATG 6 cut(s) 208, 298, 405, 490, 545, 745
NlaIV GGNNCC 3 cut(s) 14, 171, 422
NmuCI GTSAC 1 cut(s) 490
PfeI GAWTC 1 cut(s) 383
PkrI GCNGC 2 cut(s) 9, 96
PleI GAGTC 1 cut(s) 577
PpsI GAGTC 1 cut(s) 577
Psp1406I AACGTT 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 80, 433
PspFI CCCAGC 2 cut(s) 424, 442
PspGI CCWGG 2 cut(s) 80, 433
PspN4I GGNNCC 3 cut(s) 14, 171, 422
PspPI GGNCC 1 cut(s) 12
PstNI CAGNNNCTG 1 cut(s) 434
RsaI GTAC 2 cut(s) 617, 746
RsaNI GTAC 2 cut(s) 616, 745
SaqAI TTAA 3 cut(s) 477, 726, 757
SatI GCNGC 2 cut(s) 8, 95
Sau3AI GATC 4 cut(s) 207, 373, 544, 691
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 1 cut(s) 577
ScrFI CCNGG 2 cut(s) 82, 435
SduI GDGCHC 1 cut(s) 425
SfaNI GCATC 1 cut(s) 289
SinI GGWCC 1 cut(s) 12
SpeI ACTAGT 1 cut(s) 62
Sse9I AATT 7 cut(s) 174, 235, 252, 522, 573, 638, 660
SsiI CCGC 6 cut(s) 10, 16, 95, 134, 531, 718
SspMI CTAG 2 cut(s) 63, 515
StyD4I CCNGG 2 cut(s) 80, 433
StyI CCWWGG 2 cut(s) 54, 514
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 3 cut(s) 195, 318, 630
TaqI TCGA 1 cut(s) 195
TasI AATT 7 cut(s) 174, 235, 252, 522, 573, 638, 660
TatI WGTACW 2 cut(s) 615, 744
TauI GCSGC 1 cut(s) 97
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 3 cut(s) 477, 726, 757
Tru9I TTAA 3 cut(s) 477, 726, 757
TscAI CASTG 2 cut(s) 185, 689
TseFI GTSAC 1 cut(s) 490
TseI GCWGC 1 cut(s) 7
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 5 cut(s) 237, 375, 475, 519, 579
TspRI CASTG 2 cut(s) 185, 689
VpaK11BI GGWCC 1 cut(s) 12
XapI RAATTY 2 cut(s) 252, 660
XmaJI CCTAGG 1 cut(s) 514
XspI CTAG 2 cut(s) 63, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.