RchiOBHm_Chr3g0460101

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0006132
Physical Location & Seq
Reverse (-)
8339613 .. 8340061
449 bp
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UTR
Exon/CDS
Intron
PRQ42664

Sequence Viewer

Length: 381 bp
ATGATTAAAGCAATGATGAAACGAACCCAGGAGGTGTTGTTTTTTCTGATTCTGGCTTTGTTCTACAAGGCCACCGGCATGAAGCCTTGCTCTAAGCCCCCGCAAGTCGACTGTGACGGCTTCTGTTTGAGCTGGAGACTGGCGGTGGAGGCCAACAATGTGCGTGGGTGGCGCACCGTGCCAACCCAGTGCTTGCACTATCTCGAAACATACATGATTGGAGGACAGTACGACCGCGACATAGAATTCATTGTTGGTGAAATTATGAGTTACGTAAACGGAATCGTTCCCTCGGATGATGGCATGGATGCATGGATCTTGGATGTGGACGACACTTGCATATCCAATGTCTTATACTACAAGGGAAAGAGATACGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

126

Amino Acids

14.45

Weight (kDa)

5.29

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 36 - 126 4.9e-24 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 237
AciI CCGC 3 cut(s) 101, 143, 235
AclWI GGATC 1 cut(s) 323
AcsI RAATTY 1 cut(s) 245
AfaI GTAC 1 cut(s) 230
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 2 cut(s) 237, 269
AluBI AGCT 1 cut(s) 132
AluI AGCT 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 1 cut(s) 323
AoxI GGCC 2 cut(s) 69, 150
ApoI RAATTY 1 cut(s) 245
AspLEI GCGC 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 269
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 130
Bme1390I CCNGG 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 29
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 1 cut(s) 298
BmuI ACTGGG 1 cut(s) 181
BpmI CTGGAG 1 cut(s) 154
BsaAI YACGTR 1 cut(s) 274
BsaJI CCNNGG 2 cut(s) 27, 291
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 2 cut(s) 144, 187
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 27, 291
BseGI GGATG 3 cut(s) 301, 313, 328
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 144, 187
Bsh1236I CGCG 1 cut(s) 237
Bsh1285I CGRYCG 1 cut(s) 235
BshFI GGCC 2 cut(s) 71, 152
BsiEI CGRYCG 1 cut(s) 235
BsiSI CCGG 1 cut(s) 75
BsmAI GTCTC 1 cut(s) 130
BsnI GGCC 2 cut(s) 71, 152
Bsp143I GATC 1 cut(s) 315
BspACI CCGC 3 cut(s) 101, 143, 235
BspANI GGCC 2 cut(s) 71, 152
BspFNI CGCG 1 cut(s) 237
BspPI GGATC 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 2 cut(s) 144, 187
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 27, 291
BssMI GATC 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 3 cut(s) 113, 178, 228
BstBAI YACGTR 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 194
BstDEI CTNAG 1 cut(s) 93
BstF5I GGATG 3 cut(s) 301, 313, 328
BstFNI CGCG 1 cut(s) 237
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 1 cut(s) 318
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 1 cut(s) 315
BstMCI CGRYCG 1 cut(s) 235
BstMWI GCNNNNNNNGC 3 cut(s) 149, 169, 178
BstNI CCWGG 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 27
BstSNI TACGTA 1 cut(s) 274
BstUI CGCG 1 cut(s) 237
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BsuRI GGCC 2 cut(s) 71, 152
BtsCI GGATG 3 cut(s) 301, 313, 328
BtsIMutI CAGTG 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 194
CfoI GCGC 1 cut(s) 174
Cfr10I RCCGGY 1 cut(s) 74
Csp6I GTAC 1 cut(s) 229
CspCI CAANNNNNGTGG 2 cut(s) 145, 180
CviAII CATG 4 cut(s) 79, 214, 304, 312
CviJI RGCY 7 cut(s) 56, 71, 85, 97, 120, 132, 152
CviKI_1 RGCY 7 cut(s) 56, 71, 85, 97, 120, 132, 152
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 1 cut(s) 93
DpnI GATC 1 cut(s) 317
DpnII GATC 1 cut(s) 315
Eco105I TACGTA 1 cut(s) 274
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 27
EcoT22I ATGCAT 1 cut(s) 313
FaeI CATG 4 cut(s) 82, 217, 307, 315
FaiI YATR 9 cut(s) 80, 211, 215, 242, 266, 305, 313, 341, 355
FatI CATG 4 cut(s) 78, 213, 303, 311
FauI CCCGC 1 cut(s) 108
FblI GTMKAC 1 cut(s) 108
FokI GGATG 3 cut(s) 308, 320, 335
GlaI GCGC 1 cut(s) 173
GsuI CTGGAG 1 cut(s) 154
HaeIII GGCC 2 cut(s) 71, 152
HapII CCGG 1 cut(s) 75
HhaI GCGC 1 cut(s) 174
Hin1II CATG 4 cut(s) 82, 217, 307, 315
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 2 cut(s) 49, 282
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 3 cut(s) 109, 277, 328
Hpy188I TCNGA 2 cut(s) 48, 295
Hpy188III TCNNGA 1 cut(s) 203
Hpy8I GTNNAC 3 cut(s) 109, 277, 328
HpyCH4III ACNGT 3 cut(s) 113, 178, 228
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 3 cut(s) 196, 311, 339
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 169, 178
HpyF3I CTNAG 1 cut(s) 93
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 4 cut(s) 82, 217, 307, 315
HspAI GCGC 1 cut(s) 172
Kzo9I GATC 1 cut(s) 315
LpnPI CCDG 7 cut(s) 14, 38, 41, 88, 118, 125, 200
LweI GCATC 1 cut(s) 298
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 2 cut(s) 113, 269
MalI GATC 1 cut(s) 317
MboI GATC 1 cut(s) 315
MflI RGATCY 1 cut(s) 315
MluCI AATT 2 cut(s) 245, 261
MnlI CCTC 4 cut(s) 25, 142, 215, 301
Mph1103I ATGCAT 1 cut(s) 313
MseI TTAA 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 77
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 3 cut(s) 149, 169, 178
NdeII GATC 1 cut(s) 315
NlaIII CATG 4 cut(s) 82, 217, 307, 315
NmuCI GTSAC 1 cut(s) 113
NsiI ATGCAT 1 cut(s) 313
PfeI GAWTC 2 cut(s) 49, 282
Ppu21I YACGTR 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PsuI RGATCY 1 cut(s) 315
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 77
SalI GTCGAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 6
Sau3AI GATC 1 cut(s) 315
ScrFI CCNGG 1 cut(s) 29
SetI ASST 3 cut(s) 36, 134, 276
SfaNI GCATC 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 77
SnaBI TACGTA 1 cut(s) 274
Sse9I AATT 2 cut(s) 245, 261
SsiI CCGC 3 cut(s) 101, 143, 235
StyD4I CCNGG 1 cut(s) 27
TaaI ACNGT 3 cut(s) 113, 178, 228
TaiI ACGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 108, 204
TasI AATT 2 cut(s) 245, 261
TfiI GAWTC 2 cut(s) 49, 282
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 1 cut(s) 194
TseFI GTSAC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 3 cut(s) 32, 95, 238
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 1 cut(s) 194
XapI RAATTY 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.