Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0005275

Physical Location & Seq

Genomic Coordinates Reverse (-)

9381451 .. 9382425

Gene Structure 975 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g15100.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 975 bp

ATGGCGCCAAACCTAGAGTGCCGCATGTACGAAGCAAAATACCCGCAGGTAGACGCGGCGGTGATGATCCAGGTCAACAGCATCGACGACGAGAATGGCGCCTACGTCTCCCTTCTCGAGTACAACAACATGGAGGGCTTTATTCCCGTGACAGAGCTAAGCAACCGCCGCCTCCGGAGCATTCACAAACACGTCAAGGTTGGCCGCATCGAGCCCTCCCTGGTTTTCAAGGTCGACGAAACCAAGGGCTACGTCGACTTGAGCAAGCGGAGGCTCTCCCCGGAGGACATCCAGGCCTGCGACGACAAGTACAACAAGAGCAAGCTCGTCCACTCCATCATGCGCCACGTCGCCGAGACTGTGAATATCGACTTGGAGGAGTTGTACGTGAACATTGCATGGCCGCTGTACAAGAAGTACGGCCATGCAGCTGAAGCTTTCAAAATAATTGCTACGGATTCCGATTTGGTTCTCGACTCCCTCGCCCCCGCCGTGTCGGAGGAGGTGAAGGAGTGTCTGATTAAGAACATCAGGAGAAGAATGCCGGTGCAACCACTGAGAATCCGAGCAGATATTGATGTGACATGTTATCACTTCGACGGCGTTGTTCATATTAAGGACGCGCTCCGGAGGGCGGAAGCTGCCGGGAACGATGAGTGTCCGGTGAAGGTTAAGCTGATTGCTCCACCGCGCTACGTTGTTACGACGCAGACTCTGGATAAGAAGCAAGGGATATCAATTTTGAGCGAAGCTATTAAAGCCTGCAAGGAAGCAATAGAGAAGAGCAAGGGGAAACTGGTAGTGAAGGAGGTACCGAGAGTGGTGAGTGAGGTGGAGGATAAGATGCATGCAGATCAGGAGAAGAATGAGGGGGTTATGAGTAGTGGTGATGAAGGTGAGGAAGAAGAAGAAGAAGACACAGGATTGGGAGACGTGGACGTGGAGAACGCAGGCCAGGGCATTATAGTTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000077 GO:0000278 GO:0002181 GO:0002183 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0005850 GO:0005851 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0010564 GO:0010948 GO:0019538 GO:0022402 GO:0031570 GO:0031571 GO:0032991 GO:0033290 GO:0033554 GO:0034641 GO:0034645 GO:0043021 GO:0043022 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043603 GO:0043604 GO:0043614 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0044877 GO:0045786 GO:0045930 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070993 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:1990904 GO:2000045 GO:2000134

KEGG Pathways

Metabolic & Signaling

ko03013 ko04138 ko04140 ko04141 ko04210 ko04932 ko05160 ko05162 ko05164 ko05168 map03013 map04138 map04140 map04141 map04210 map04932 map05160 map05162 map05164 map05168

Pfam Domains

Protein Families

EIF_2_alpha S1

Protein Analysis

325

Amino Acids

36.35

Weight (kDa)

5.13

Isoelectric Point (pI)

59.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S1	PF00575	15 - 90	2.8e-10	S1 RNA binding domain
EIF_2_alpha	PF07541	128 - 241	1.2e-33	Eukaryotic translation initiation factor 2 alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005275)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05470
fragaria_vesca	FvH4_6g15100 FvH4_7g25050
malus_domestica	MD01G1157300.v1.1 MD07G1226100.v1.1
prunus_persica	Prupe.2G255200_v2.0.a1
pyrus_communis	pycom01g17410 pycom07g20450
rosa_chinensis	RchiOBHm_Chr1g0370911 RchiOBHm_Chr3g0468371
rosa_laevigata	RLG00000024436 RLG00000026999
rosa_multiflora	Rmu_co7996168.1_g000001 Rmu_sc0001018.1_g000002 Rmu_sc0004353.1_g000010 Rmu_sc0014003.1_g000002
rosa_roxburghii	Rroxscaffold_4G00286130 Rroxscaffold_6G00412840
rosa_rugosa	Rorug01G0360200 Rorug03G0095000
rosa_samantha	Rh1AG370200 Rh1BG333800 Rh1CG347100 Rh1DG365000 Rh3AG142800 Rh3BG165200 Rh3DG164800
rosa_wichuraiana	Rw1G032580 Rw3G013490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	37
Acc65I	GGTACC	1 cut(s)	811
AccB1I	GGYRCC	3 cut(s)	4, 98, 811
AccI	GTMKAC	3 cut(s)	51, 234, 255
AccII	CGCG	3 cut(s)	56, 623, 691
AccIII	TCCGGA	2 cut(s)	174, 627
AclWI	GGATC	1 cut(s)	61
AcoI	YGGCCR	3 cut(s)	202, 401, 421
AcuI	CTGAAG	1 cut(s)	453
AcyI	GRCGYC	2 cut(s)	5, 99
AfaI	GTAC	7 cut(s)	29, 122, 311, 386, 410, 419, 813
AfiI	CCNNNNNNNGG	1 cut(s)	634
AflIII	ACRYGT	2 cut(s)	190, 584
AgsI	TTSAA	2 cut(s)	229, 442
AjiI	CACGTC	4 cut(s)	193, 349, 934, 940
AjnI	CCWGG	4 cut(s)	69, 219, 291, 954
AjuI	GAANNNNNNNTTGG	2 cut(s)	356, 388
AluBI	AGCT	7 cut(s)	157, 325, 431, 437, 641, 676, 752
AluI	AGCT	7 cut(s)	157, 325, 431, 437, 641, 676, 752
Alw26I	GTCTC	3 cut(s)	112, 350, 924
AlwI	GGATC	1 cut(s)	61
AlwNI	CAGNNNCTG	1 cut(s)	715
Ama87I	CYCGRG	1 cut(s)	116
Aor13HI	TCCGGA	2 cut(s)	174, 627
AoxI	GGCC	5 cut(s)	202, 294, 401, 421, 952
ApeKI	GCWGC	2 cut(s)	428, 641
Asp718I	GGTACC	1 cut(s)	811
AspLEI	GCGC	5 cut(s)	7, 101, 345, 625, 693
AsuC2I	CCSGG	2 cut(s)	281, 646
AsuHPI	GGTGA	6 cut(s)	73, 517, 676, 835, 899, 908
AvaI	CYCGRG	1 cut(s)	116
BanI	GGYRCC	3 cut(s)	4, 98, 811
BanII	GRGCYC	1 cut(s)	216
BbsI	GAAGAC	1 cut(s)	921
BbvI	GCAGC	2 cut(s)	440, 628
BccI	CCATC	1 cut(s)	344
BceAI	ACGGC	3 cut(s)	436, 476, 616
BciT130I	CCWGG	4 cut(s)	71, 221, 293, 956
BcnI	CCSGG	2 cut(s)	281, 646
BcoDI	GTCTC	3 cut(s)	112, 350, 924
BfaI	CTAG	1 cut(s)	14
BfoI	RGCGCY	2 cut(s)	8, 102
BfuAI	ACCTGC	1 cut(s)	37
BisI	GCNGC	7 cut(s)	22, 57, 169, 205, 404, 429, 642
BlpI	GCTNAGC	1 cut(s)	158
BlsI	GCNGC	7 cut(s)	23, 58, 170, 206, 405, 430, 643
Bme1390I	CCNGG	6 cut(s)	71, 221, 281, 293, 646, 956
BmeT110I	CYCGRG	1 cut(s)	116
BmgBI	CACGTC	4 cut(s)	193, 349, 934, 940
BmiI	GGNNCC	3 cut(s)	6, 100, 813
BmrFI	CCNGG	6 cut(s)	71, 221, 281, 293, 646, 956
BmsI	GCATC	3 cut(s)	90, 216, 834
BpiI	GAAGAC	1 cut(s)	921
Bpu1102I	GCTNAGC	1 cut(s)	158
BpuEI	CTTGAG	1 cut(s)	280
BpuMI	CCSGG	2 cut(s)	281, 646
BsaAI	YACGTR	1 cut(s)	388
BsaHI	GRCGYC	2 cut(s)	5, 99
BsaJI	CCNNGG	4 cut(s)	219, 243, 279, 955
BsaWI	WCCGGW	3 cut(s)	174, 627, 661
BsaXI	ACNNNNNCTCC	2 cut(s)	368, 398
Bsc4I	CCNNNNNNNGG	1 cut(s)	634
Bse118I	RCCGGY	1 cut(s)	544
Bse1I	ACTGG	1 cut(s)	801
Bse3DI	GCAATG	1 cut(s)	393
BseAI	TCCGGA	2 cut(s)	174, 627
BseBI	CCWGG	4 cut(s)	71, 221, 293, 956
BseDI	CCNNGG	4 cut(s)	219, 243, 279, 955
BseGI	GGATG	1 cut(s)	288
BseLI	CCNNNNNNNGG	1 cut(s)	634
BseMI	GCAATG	1 cut(s)	393
BseMII	CTCAG	1 cut(s)	548
BseNI	ACTGG	1 cut(s)	801
BseRI	GAGGAG	2 cut(s)	392, 515
BseXI	GCAGC	2 cut(s)	440, 628
Bsh1236I	CGCG	3 cut(s)	56, 623, 691
BshFI	GGCC	5 cut(s)	204, 296, 403, 423, 954
BshNI	GGYRCC	3 cut(s)	4, 98, 811
BsiHKCI	CYCGRG	1 cut(s)	116
BsiSI	CCGG	6 cut(s)	175, 281, 545, 628, 645, 662
BslI	CCNNNNNNNGG	1 cut(s)	634
BsmAI	GTCTC	3 cut(s)	112, 350, 924
BsmBI	CGTCTC	2 cut(s)	112, 924
BsmI	GAATGC	2 cut(s)	180, 546
BsnI	GGCC	5 cut(s)	204, 296, 403, 423, 954
BsoBI	CYCGRG	1 cut(s)	116
Bsp1286I	GDGCHC	1 cut(s)	216
Bsp13I	TCCGGA	2 cut(s)	174, 627
Bsp1407I	TGTACA	1 cut(s)	408
Bsp143I	GATC	2 cut(s)	66, 853
Bsp1720I	GCTNAGC	1 cut(s)	158
BspANI	GGCC	5 cut(s)	204, 296, 403, 423, 954
BspCNI	CTCAG	1 cut(s)	549
BspEI	TCCGGA	2 cut(s)	174, 627
BspFNI	CGCG	3 cut(s)	56, 623, 691
BspLI	GGNNCC	3 cut(s)	6, 100, 813
BspMI	ACCTGC	1 cut(s)	37
BspPI	GGATC	1 cut(s)	61
BspQI	GCTCTTC	1 cut(s)	776
BspT107I	GGYRCC	3 cut(s)	4, 98, 811
BsrDI	GCAATG	1 cut(s)	393
BsrFI	RCCGGY	1 cut(s)	544
BsrGI	TGTACA	1 cut(s)	408
BsrI	ACTGG	1 cut(s)	801
BssAI	RCCGGY	1 cut(s)	544
BssECI	CCNNGG	4 cut(s)	219, 243, 279, 955
BssMI	GATC	2 cut(s)	66, 853
BssNI	GRCGYC	2 cut(s)	5, 99
BssT1I	CCWWGG	1 cut(s)	243
Bst2UI	CCWGG	4 cut(s)	71, 221, 293, 956
Bst4CI	ACNGT	1 cut(s)	361
Bst6I	CTCTTC	1 cut(s)	776
BstACI	GRCGYC	2 cut(s)	5, 99
BstAUI	TGTACA	1 cut(s)	408
BstBAI	YACGTR	1 cut(s)	388
BstC8I	GCNNGC	6 cut(s)	266, 298, 323, 763, 849, 952
BstDEI	CTNAG	2 cut(s)	158, 557
BstF5I	GGATG	1 cut(s)	288
BstFNI	CGCG	3 cut(s)	56, 623, 691
BstH2I	RGCGCY	2 cut(s)	8, 102
BstHHI	GCGC	5 cut(s)	7, 101, 345, 625, 693
BstKTI	GATC	2 cut(s)	69, 856
BstMAI	GTCTC	3 cut(s)	112, 350, 924
BstMBI	GATC	2 cut(s)	66, 853
BstMWI	GCNNNNNNNGC	5 cut(s)	168, 177, 434, 641, 758
BstNI	CCWGG	4 cut(s)	71, 221, 293, 956
BstNSI	RCATGY	3 cut(s)	28, 588, 851
BstSCI	CCNGG	6 cut(s)	69, 219, 279, 291, 644, 954
BstUI	CGCG	3 cut(s)	56, 623, 691
BstV1I	GCAGC	2 cut(s)	440, 628
BstV2I	GAAGAC	1 cut(s)	921
BsuRI	GGCC	5 cut(s)	204, 296, 403, 423, 954
BtrI	CACGTC	4 cut(s)	193, 349, 934, 940
BtsCI	GGATG	1 cut(s)	288
BtsIMutI	CAGTG	1 cut(s)	554
BveI	ACCTGC	1 cut(s)	37
Cac8I	GCNNGC	6 cut(s)	266, 298, 323, 763, 849, 952
CaiI	CAGNNNCTG	1 cut(s)	715
CfoI	GCGC	5 cut(s)	7, 101, 345, 625, 693
Cfr10I	RCCGGY	1 cut(s)	544
CseI	GACGC	3 cut(s)	62, 629, 715
Csp6I	GTAC	7 cut(s)	28, 121, 310, 385, 409, 418, 812
CviAII	CATG	7 cut(s)	25, 130, 340, 399, 425, 585, 848
CviQI	GTAC	7 cut(s)	28, 121, 310, 385, 409, 418, 812
DdeI	CTNAG	2 cut(s)	158, 557
DinI	GGCGCC	2 cut(s)	6, 100
DpnI	GATC	2 cut(s)	68, 855
DpnII	GATC	2 cut(s)	66, 853
EaeI	YGGCCR	3 cut(s)	202, 401, 421
Eam1104I	CTCTTC	1 cut(s)	776
EarI	CTCTTC	1 cut(s)	776
EciI	GGCGGA	1 cut(s)	650
Eco130I	CCWWGG	1 cut(s)	243
Eco147I	AGGCCT	1 cut(s)	296
Eco24I	GRGCYC	1 cut(s)	216
Eco32I	GATATC	1 cut(s)	735
Eco57I	CTGAAG	1 cut(s)	453
Eco88I	CYCGRG	1 cut(s)	116
EcoRII	CCWGG	4 cut(s)	69, 219, 291, 954
EcoRV	GATATC	1 cut(s)	735
EcoT14I	CCWWGG	1 cut(s)	243
EcoT22I	ATGCAT	1 cut(s)	849
EcoT38I	GRGCYC	1 cut(s)	216
EgeI	GGCGCC	2 cut(s)	6, 100
EheI	GGCGCC	2 cut(s)	6, 100
ErhI	CCWWGG	1 cut(s)	243
Esp3I	CGTCTC	2 cut(s)	112, 924
FaeI	CATG	7 cut(s)	28, 133, 343, 402, 428, 588, 851
FatI	CATG	7 cut(s)	24, 129, 339, 398, 424, 584, 847
FauI	CCCGC	2 cut(s)	51, 496
FblI	GTMKAC	3 cut(s)	51, 234, 255
Fnu4HI	GCNGC	7 cut(s)	22, 57, 169, 205, 404, 429, 642
FokI	GGATG	1 cut(s)	275
FriOI	GRGCYC	1 cut(s)	216
Fsp4HI	GCNGC	7 cut(s)	22, 57, 169, 205, 404, 429, 642
FspBI	CTAG	1 cut(s)	14
GlaI	GCGC	5 cut(s)	6, 100, 344, 624, 692
GluI	GCNGC	7 cut(s)	22, 57, 169, 205, 404, 429, 642
HaeII	RGCGCY	2 cut(s)	8, 102
HaeIII	GGCC	5 cut(s)	204, 296, 403, 423, 954
HapII	CCGG	6 cut(s)	175, 281, 545, 628, 645, 662
HgaI	GACGC	3 cut(s)	62, 629, 715
HhaI	GCGC	5 cut(s)	7, 101, 345, 625, 693
Hin1I	GRCGYC	2 cut(s)	5, 99
Hin1II	CATG	7 cut(s)	28, 133, 343, 402, 428, 588, 851
Hin6I	GCGC	5 cut(s)	5, 99, 343, 623, 691
HinP1I	GCGC	5 cut(s)	5, 99, 343, 623, 691
HincII	GTYRAC	3 cut(s)	76, 235, 256
HindII	GTYRAC	3 cut(s)	76, 235, 256
HindIII	AAGCTT	1 cut(s)	435
HinfI	GANTC	4 cut(s)	458, 476, 561, 712
HpaII	CCGG	6 cut(s)	175, 281, 545, 628, 645, 662
HphI	GGTGA	6 cut(s)	73, 517, 676, 835, 899, 908
Hpy166II	GTNNAC	7 cut(s)	52, 76, 235, 256, 331, 391, 937
Hpy188I	TCNGA	4 cut(s)	463, 499, 519, 566
Hpy188III	TCNNGA	7 cut(s)	116, 175, 473, 532, 628, 716, 857
Hpy8I	GTNNAC	7 cut(s)	52, 76, 235, 256, 331, 391, 937
Hpy99I	CGWCG	8 cut(s)	89, 92, 239, 257, 305, 353, 602, 709
HpyAV	CCTTC	5 cut(s)	122, 502, 661, 799, 887
HpyCH4III	ACNGT	1 cut(s)	361
HpyCH4IV	ACGT	8 cut(s)	105, 192, 252, 348, 387, 696, 933, 939
HpyCH4V	TGCA	6 cut(s)	398, 428, 550, 765, 847, 851
HpyF10VI	GCNNNNNNNGC	5 cut(s)	168, 177, 434, 641, 758
HpyF3I	CTNAG	2 cut(s)	158, 557
HpySE526I	ACGT	8 cut(s)	105, 192, 252, 348, 387, 696, 933, 939
Hsp92I	GRCGYC	2 cut(s)	5, 99
Hsp92II	CATG	7 cut(s)	28, 133, 343, 402, 428, 588, 851
HspAI	GCGC	5 cut(s)	5, 99, 343, 623, 691
KasI	GGCGCC	2 cut(s)	4, 98
Kpn2I	TCCGGA	2 cut(s)	174, 627
KpnI	GGTACC	1 cut(s)	815
Kzo9I	GATC	2 cut(s)	66, 853
LguI	GCTCTTC	1 cut(s)	776
LmnI	GCTCC	3 cut(s)	177, 630, 688
Lsp1109I	GCAGC	2 cut(s)	440, 628
LweI	GCATC	3 cut(s)	90, 216, 834
MaeI	CTAG	1 cut(s)	14
MaeII	ACGT	8 cut(s)	105, 192, 252, 348, 387, 696, 933, 939
MaeIII	GTNAC	3 cut(s)	148, 580, 700
MalI	GATC	2 cut(s)	68, 855
MboI	GATC	2 cut(s)	66, 853
MboII	GAAGA	8 cut(s)	549, 793, 874, 914, 917, 920, 923, 926
MhlI	GDGCHC	1 cut(s)	216
MluCI	AATT	2 cut(s)	447, 738
Mly113I	GGCGCC	2 cut(s)	5, 99
MlyI	GAGTC	2 cut(s)	470, 706
MmeI	TCCRAC	1 cut(s)	477
Mph1103I	ATGCAT	1 cut(s)	849
MroI	TCCGGA	2 cut(s)	174, 627
MseI	TTAA	4 cut(s)	522, 615, 672, 756
MspA1I	CMGCKG	2 cut(s)	406, 431
MspI	CCGG	6 cut(s)	175, 281, 545, 628, 645, 662
MspR9I	CCNGG	6 cut(s)	71, 221, 281, 293, 646, 956
Mva1269I	GAATGC	2 cut(s)	180, 546
MvaI	CCWGG	4 cut(s)	71, 221, 293, 956
MvnI	CGCG	3 cut(s)	56, 623, 691
MwoI	GCNNNNNNNGC	5 cut(s)	168, 177, 434, 641, 758
NarI	GGCGCC	2 cut(s)	5, 99
NciI	CCSGG	2 cut(s)	281, 646
NdeII	GATC	2 cut(s)	66, 853
NlaIII	CATG	7 cut(s)	28, 133, 343, 402, 428, 588, 851
NlaIV	GGNNCC	3 cut(s)	6, 100, 813
NmeAIII	GCCGAG	1 cut(s)	379
NmuCI	GTSAC	2 cut(s)	148, 580
NsiI	ATGCAT	1 cut(s)	849
NspI	RCATGY	3 cut(s)	28, 588, 851
PaeI	GCATGC	1 cut(s)	851
PaeR7I	CTCGAG	1 cut(s)	116
PceI	AGGCCT	1 cut(s)	296
PciI	ACATGT	1 cut(s)	584
PciSI	GCTCTTC	1 cut(s)	776
PcsI	WCGNNNNNNNCGW	1 cut(s)	489
PctI	GAATGC	2 cut(s)	180, 546
PfeI	GAWTC	2 cut(s)	458, 561
PkrI	GCNGC	7 cut(s)	23, 58, 170, 206, 405, 430, 643
PleI	GAGTC	2 cut(s)	470, 706
PluTI	GGCGCC	2 cut(s)	8, 102
PpsI	GAGTC	2 cut(s)	470, 706
Ppu21I	YACGTR	1 cut(s)	388
PscI	ACATGT	1 cut(s)	584
Psp6I	CCWGG	4 cut(s)	69, 219, 291, 954
PspGI	CCWGG	4 cut(s)	69, 219, 291, 954
PspN4I	GGNNCC	3 cut(s)	6, 100, 813
PstNI	CAGNNNCTG	1 cut(s)	715
PvuII	CAGCTG	1 cut(s)	431
RsaI	GTAC	7 cut(s)	29, 122, 311, 386, 410, 419, 813
RsaNI	GTAC	7 cut(s)	28, 121, 310, 385, 409, 418, 812
SalI	GTCGAC	2 cut(s)	233, 254
SapI	GCTCTTC	1 cut(s)	776
SaqAI	TTAA	4 cut(s)	522, 615, 672, 756
SatI	GCNGC	7 cut(s)	22, 57, 169, 205, 404, 429, 642
Sau3AI	GATC	2 cut(s)	66, 853
SchI	GAGTC	2 cut(s)	470, 706
ScrFI	CCNGG	6 cut(s)	71, 221, 281, 293, 646, 956
SduI	GDGCHC	1 cut(s)	216
SfaNI	GCATC	3 cut(s)	90, 216, 834
SfoI	GGCGCC	2 cut(s)	6, 100
Sfr274I	CTCGAG	1 cut(s)	116
SlaI	CTCGAG	1 cut(s)	116
SmlI	CTYRAG	2 cut(s)	116, 259
SmoI	CTYRAG	2 cut(s)	116, 259
SphI	GCATGC	1 cut(s)	851
Sse9I	AATT	2 cut(s)	447, 738
SseBI	AGGCCT	1 cut(s)	296
SspDI	GGCGCC	2 cut(s)	4, 98
SspMI	CTAG	1 cut(s)	14
StuI	AGGCCT	1 cut(s)	296
StyD4I	CCNGG	6 cut(s)	69, 219, 279, 291, 644, 954
StyI	CCWWGG	1 cut(s)	243
TaaI	ACNGT	1 cut(s)	361
TaiI	ACGT	8 cut(s)	108, 195, 255, 351, 390, 699, 936, 942
TaqI	TCGA	8 cut(s)	84, 117, 210, 234, 255, 369, 474, 597
TasI	AATT	2 cut(s)	447, 738
TatI	WGTACW	3 cut(s)	120, 309, 408
TauI	GCSGC	5 cut(s)	24, 59, 171, 207, 406
TfiI	GAWTC	2 cut(s)	458, 561
Tru1I	TTAA	4 cut(s)	522, 615, 672, 756
Tru9I	TTAA	4 cut(s)	522, 615, 672, 756
TscAI	CASTG	1 cut(s)	561
TseFI	GTSAC	2 cut(s)	148, 580
TseI	GCWGC	2 cut(s)	428, 641
Tsp45I	GTSAC	2 cut(s)	148, 580
TspDTI	ATGAA	2 cut(s)	599, 906
TspGWI	ACGGA	1 cut(s)	470
TspRI	CASTG	1 cut(s)	561
XceI	RCATGY	3 cut(s)	28, 588, 851
XhoI	CTCGAG	1 cut(s)	116
XmiI	GTMKAC	3 cut(s)	51, 234, 255
XspI	CTAG	1 cut(s)	14
Zsp2I	ATGCAT	1 cut(s)	849

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.