FvH4_6g20900

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004240
Physical Location & Seq
Reverse (-)
14390785 .. 14395549
4765 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20900.t2

Sequence Viewer

Length: 1152 bp
ATGACGGAGGCAAAGAAGGATGAAAGTCAATTGATATCAGCTGCTGCTTTTGTTGAAGGGGGAATACAAGATGCATGTGGTGAAGCCTGTAGCATATGCCTTGAGGATTTTTGTGAAAGTGATCCTTCAGTGCTGACTACTTGCAAGCATGAGTTTCACTTCCACTGCATCCTTGAATGGTGCCAGAGAAGTTCCCAATGTCCGATGTGTTGGCAGGGCCTTAGCTTGAAGGATCCTTCCAGTCAAGAATTACTTGAGGCTGTAGAACGGGAGAGGACCATTAGGGCCACAGCAAGAAGTGCAACCATATTTCTTCATCCCACCCTCAGCAATTTTGAAGTGCAGCATTTAGCTGATGGTACTAATGATGCTGACCTTGAAGAGCGTTTCATCCAGCATTTGGCAGCTGCTGCATCAATGGGAAGGACTCACCACATTGGTAGAAGGGAAGGCCAGCGGAGCCAGTCATCTCTTCGTGGTCATCCACACTTCTCGGTCATTTCCACTCACCCCAGTGCACATTCTCTTGGTCATGTTTCTGCCTCGGGTGGGGAGAGTGAACCAGCTACCATAACTGCAGCAAGTCCATCTACCCCACTTACATCAGGTGGAGATGAATCATCACGATGGAGTTCACAAGTTTCTTCTGTTCAAACTGATGAATTTTCTTCCTCCACATCTAGGCCTACACTCATGCAACGAAATCGTCAAGGAATTCCCTCTACCGGCTGGAGCTCTGCCAGTCACTCCTCACCAATAAATCAGGAAACAGCAGGACCATCAGATCTTCAGTCTATTTCAGAGTCTCTGAAATCAAAATTTAATGCCATGTCAATGAAATACAAGGAGTCATTTTCAAAGAGTACAAGAGGATGGAAGGAGAGGCTGTTCTCTCGTAGCCCTTCTATGTCAGAACTTGGTTCTGAAGTCCGTAGAGAAGTCAATGCTGGAATCGCTAGTGTATCTCGAATTATAGAGAGCCTTGAGACCAGAGATAATAGTAAAGTTGGTCAGGATCGTGTAGCAAATCAAACAGCAGATGGTTCTACGACGGACCAGGGCAACCGGAACAGCTCTGAGAGTCATGGGAGGACTTTGACTGACAACAATAGGCCTGTGACACCTTGTGCTGTAGGTTCTGCTTCCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

384

Amino Acids

41.48

Weight (kDa)

5.93

Isoelectric Point (pI)

55.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 48 - 92 1.3e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 51 - 92 7.7e-10 Ring finger domain
zf-RING_11 PF17123 52 - 79 9.4e-07 RING-like zinc finger
zf-C3HC4 PF00097 52 - 92 6.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 63 - 99 3e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 180
AccB7I CCANNNNNTGG 1 cut(s) 400
AciI CCGC 1 cut(s) 457
AclWI GGATC 4 cut(s) 116, 227, 240, 1023
AcsI RAATTY 3 cut(s) 662, 714, 818
AcuI CTGAAG 3 cut(s) 111, 773, 945
AdeI CACNNNGTG 1 cut(s) 1127
AfaI GTAC 2 cut(s) 361, 865
AfiI CCNNNNNNNGG 4 cut(s) 400, 549, 681, 725
AgsI TTSAA 7 cut(s) 56, 176, 229, 338, 380, 653, 858
AjnI CCWGG 1 cut(s) 1056
AleI CACNNNNGTG 1 cut(s) 513
AloI GAACNNNNNNTCC 2 cut(s) 872, 904
AluBI AGCT 7 cut(s) 41, 225, 353, 407, 566, 735, 1074
AluI AGCT 7 cut(s) 41, 225, 353, 407, 566, 735, 1074
Alw21I GWGCWC 2 cut(s) 520, 737
Alw26I GTCTC 2 cut(s) 810, 980
Alw44I GTGCAC 1 cut(s) 516
AlwI GGATC 4 cut(s) 116, 227, 240, 1023
AlwNI CAGNNNCTG 2 cut(s) 44, 410
Ama87I CYCGRG 1 cut(s) 544
AoxI GGCC 5 cut(s) 217, 285, 451, 683, 1112
ApaLI GTGCAC 1 cut(s) 516
ApeKI GCWGC 7 cut(s) 41, 44, 343, 404, 407, 410, 578
ApoI RAATTY 3 cut(s) 662, 714, 818
AspS9I GGNCC 5 cut(s) 217, 276, 285, 776, 1054
AsuHPI GGTGA 4 cut(s) 92, 422, 500, 744
AvaI CYCGRG 1 cut(s) 544
AvaII GGWCC 3 cut(s) 276, 776, 1054
BaeGI GKGCMC 1 cut(s) 520
BamHI GGATCC 1 cut(s) 232
BanI GGYRCC 1 cut(s) 180
BanII GRGCYC 1 cut(s) 737
BarI GAAGNNNNNNTAC 2 cut(s) 48, 80
Bbv12I GWGCWC 2 cut(s) 520, 737
BbvCI CCTCAGC 1 cut(s) 326
BbvI GCAGC 7 cut(s) 28, 31, 355, 394, 397, 416, 590
BccI CCATC 6 cut(s) 350, 595, 621, 787, 867, 1034
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 1 cut(s) 1058
BcoDI GTCTC 2 cut(s) 810, 980
BfaI CTAG 2 cut(s) 681, 957
BfmI CTRYAG 4 cut(s) 88, 261, 576, 1131
BglII AGATCT 1 cut(s) 784
BisI GCNGC 7 cut(s) 42, 45, 344, 405, 408, 411, 579
BlsI GCNGC 7 cut(s) 43, 46, 345, 406, 409, 412, 580
Bme1390I CCNGG 1 cut(s) 1058
Bme18I GGWCC 3 cut(s) 276, 776, 1054
BmeT110I CYCGRG 1 cut(s) 544
BmgT120I GGNCC 5 cut(s) 217, 276, 285, 776, 1054
BmiI GGNNCC 3 cut(s) 182, 234, 461
BmrFI CCNGG 1 cut(s) 1058
BmrI ACTGGG 1 cut(s) 507
BmsI GCATC 4 cut(s) 61, 177, 358, 422
BmuI ACTGGG 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 751
Bpu10I CCTNAGC 2 cut(s) 221, 326
BpuEI CTTGAG 3 cut(s) 122, 275, 1004
BsaI GGTCTC 1 cut(s) 980
BsaJI CCNNGG 2 cut(s) 543, 1057
BsaWI WCCGGW 1 cut(s) 1065
BsaXI ACNNNNNCTCC 2 cut(s) 872, 902
Bsc4I CCNNNNNNNGG 4 cut(s) 400, 549, 681, 725
Bse118I RCCGGY 1 cut(s) 725
Bse1I ACTGG 4 cut(s) 240, 463, 513, 741
BseBI CCWGG 1 cut(s) 1058
BseDI CCNNGG 2 cut(s) 543, 1057
BseGI GGATG 6 cut(s) 25, 168, 316, 390, 481, 878
BseLI CCNNNNNNNGG 4 cut(s) 400, 549, 681, 725
BseMII CTCAG 2 cut(s) 340, 1068
BseNI ACTGG 4 cut(s) 240, 463, 513, 741
BseRI GAGGAG 1 cut(s) 739
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 7 cut(s) 28, 31, 355, 394, 397, 416, 590
BsgI GTGCAG 1 cut(s) 362
BshFI GGCC 5 cut(s) 219, 287, 453, 685, 1114
BshNI GGYRCC 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 520, 737
BsiHKCI CYCGRG 1 cut(s) 544
BsiSI CCGG 2 cut(s) 726, 1066
BslI CCNNNNNNNGG 4 cut(s) 400, 549, 681, 725
BsmAI GTCTC 2 cut(s) 810, 980
BsnI GGCC 5 cut(s) 219, 287, 453, 685, 1114
Bso31I GGTCTC 1 cut(s) 980
BsoBI CYCGRG 1 cut(s) 544
Bsp1286I GDGCHC 2 cut(s) 520, 737
Bsp143I GATC 4 cut(s) 121, 232, 784, 1015
BspACI CCGC 1 cut(s) 457
BspANI GGCC 5 cut(s) 219, 287, 453, 685, 1114
BspCNI CTCAG 2 cut(s) 339, 1069
BspLI GGNNCC 3 cut(s) 182, 234, 461
BspMAI CTGCAG 1 cut(s) 580
BspPI GGATC 4 cut(s) 116, 227, 240, 1023
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 980
BsrFI RCCGGY 1 cut(s) 725
BsrI ACTGG 4 cut(s) 240, 463, 513, 741
BssAI RCCGGY 1 cut(s) 725
BssECI CCNNGG 2 cut(s) 543, 1057
BssMI GATC 4 cut(s) 121, 232, 784, 1015
Bst2UI CCWGG 1 cut(s) 1058
Bst6I CTCTTC 2 cut(s) 375, 477
BstAPI GCANNNNNTGC 2 cut(s) 299, 410
BstC8I GCNNGC 2 cut(s) 146, 455
BstDEI CTNAG 3 cut(s) 221, 326, 1077
BstF5I GGATG 6 cut(s) 25, 168, 316, 390, 481, 878
BstKTI GATC 4 cut(s) 124, 235, 787, 1018
BstMAI GTCTC 2 cut(s) 810, 980
BstMBI GATC 4 cut(s) 121, 232, 784, 1015
BstMWI GCNNNNNNNGC 4 cut(s) 299, 410, 459, 953
BstNI CCWGG 1 cut(s) 1058
BstNSI RCATGY 1 cut(s) 78
BstSCI CCNGG 1 cut(s) 1056
BstSFI CTRYAG 4 cut(s) 88, 261, 576, 1131
BstSLI GKGCMC 1 cut(s) 520
BstV1I GCAGC 7 cut(s) 28, 31, 355, 394, 397, 416, 590
BstX2I RGATCY 2 cut(s) 232, 784
BstYI RGATCY 2 cut(s) 232, 784
BsuRI GGCC 5 cut(s) 219, 287, 453, 685, 1114
BtsCI GGATG 6 cut(s) 25, 168, 316, 390, 481, 878
BtsI GCAGTG 1 cut(s) 163
BtsIMutI CAGTG 3 cut(s) 135, 163, 520
Cac8I GCNNGC 2 cut(s) 146, 455
CaiI CAGNNNCTG 2 cut(s) 44, 410
Cfr10I RCCGGY 1 cut(s) 725
Cfr13I GGNCC 5 cut(s) 217, 276, 285, 776, 1054
Csp6I GTAC 2 cut(s) 360, 864
CviAII CATG 6 cut(s) 75, 149, 533, 694, 829, 1085
CviQI GTAC 2 cut(s) 360, 864
DdeI CTNAG 3 cut(s) 221, 326, 1077
DpnI GATC 4 cut(s) 123, 234, 786, 1017
DpnII GATC 4 cut(s) 121, 232, 784, 1015
DraIII CACNNNGTG 1 cut(s) 1127
Eam1104I CTCTTC 2 cut(s) 375, 477
EarI CTCTTC 2 cut(s) 375, 477
Ecl136II GAGCTC 1 cut(s) 735
Eco147I AGGCCT 2 cut(s) 685, 1114
Eco24I GRGCYC 1 cut(s) 737
Eco31I GGTCTC 1 cut(s) 980
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 3 cut(s) 276, 776, 1054
Eco53kI GAGCTC 1 cut(s) 735
Eco57I CTGAAG 3 cut(s) 111, 773, 945
Eco88I CYCGRG 1 cut(s) 544
EcoICRI GAGCTC 1 cut(s) 735
EcoO109I RGGNCCY 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 1 cut(s) 1056
EcoRV GATATC 1 cut(s) 36
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 1 cut(s) 737
FaeI CATG 6 cut(s) 78, 152, 536, 697, 832, 1088
FalI AAGNNNNNCTT 4 cut(s) 109, 141, 237, 269
FatI CATG 6 cut(s) 74, 148, 532, 693, 828, 1084
FauNDI CATATG 1 cut(s) 95
Fnu4HI GCNGC 7 cut(s) 42, 45, 344, 405, 408, 411, 579
FokI GGATG 6 cut(s) 32, 155, 303, 377, 468, 885
FriOI GRGCYC 1 cut(s) 737
Fsp4HI GCNGC 7 cut(s) 42, 45, 344, 405, 408, 411, 579
FspBI CTAG 2 cut(s) 681, 957
GluI GCNGC 7 cut(s) 42, 45, 344, 405, 408, 411, 579
GsuI CTGGAG 1 cut(s) 751
HaeIII GGCC 5 cut(s) 219, 287, 453, 685, 1114
HapII CCGG 2 cut(s) 726, 1066
Hin1II CATG 6 cut(s) 78, 152, 536, 697, 832, 1088
HinfI GANTC 6 cut(s) 427, 617, 803, 848, 951, 1081
HpaII CCGG 2 cut(s) 726, 1066
HphI GGTGA 4 cut(s) 92, 422, 500, 744
Hpy166II GTNNAC 3 cut(s) 518, 560, 635
Hpy188I TCNGA 7 cut(s) 204, 784, 802, 810, 913, 925, 1078
Hpy188III TCNNGA 5 cut(s) 245, 624, 764, 966, 1013
Hpy8I GTNNAC 3 cut(s) 518, 560, 635
Hpy99I CGWCG 1 cut(s) 1054
HpyCH4V TGCA 9 cut(s) 74, 144, 168, 302, 343, 413, 518, 578, 697
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 410, 459, 953
HpyF3I CTNAG 3 cut(s) 221, 326, 1077
Hsp92II CATG 6 cut(s) 78, 152, 536, 697, 832, 1088
Kzo9I GATC 4 cut(s) 121, 232, 784, 1015
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 2 cut(s) 459, 732
Lsp1109I GCAGC 7 cut(s) 28, 31, 355, 394, 397, 416, 590
LweI GCATC 4 cut(s) 61, 177, 358, 422
MaeI CTAG 2 cut(s) 681, 957
MaeIII GTNAC 2 cut(s) 743, 1117
MalI GATC 4 cut(s) 123, 234, 786, 1017
MboI GATC 4 cut(s) 121, 232, 784, 1015
MboII GAAGA 6 cut(s) 305, 392, 464, 636, 660, 779
MfeI CAATTG 2 cut(s) 29, 1147
MflI RGATCY 2 cut(s) 232, 784
MhlI GDGCHC 2 cut(s) 520, 737
MluCI AATT 8 cut(s) 29, 248, 331, 662, 714, 818, 969, 1147
MlyI GAGTC 4 cut(s) 421, 812, 857, 1090
Mph1103I ATGCAT 1 cut(s) 76
MseI TTAA 1 cut(s) 822
MslI CAYNNNNRTG 3 cut(s) 513, 625, 833
MspA1I CMGCKG 3 cut(s) 41, 407, 457
MspI CCGG 2 cut(s) 726, 1066
MspR9I CCNGG 1 cut(s) 1058
MunI CAATTG 2 cut(s) 29, 1147
MvaI CCWGG 1 cut(s) 1058
MwoI GCNNNNNNNGC 4 cut(s) 299, 410, 459, 953
NdeI CATATG 1 cut(s) 95
NdeII GATC 4 cut(s) 121, 232, 784, 1015
NlaIII CATG 6 cut(s) 78, 152, 536, 697, 832, 1088
NlaIV GGNNCC 3 cut(s) 182, 234, 461
NmuCI GTSAC 2 cut(s) 743, 1117
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 1 cut(s) 78
OliI CACNNNNGTG 1 cut(s) 513
PceI AGGCCT 2 cut(s) 685, 1114
PciSI GCTCTTC 1 cut(s) 375
PfeI GAWTC 2 cut(s) 617, 951
PflMI CCANNNNNTGG 1 cut(s) 400
PkrI GCNGC 7 cut(s) 43, 46, 345, 406, 409, 412, 580
PleI GAGTC 4 cut(s) 421, 811, 856, 1089
PpsI GAGTC 4 cut(s) 421, 811, 856, 1089
Psp124BI GAGCTC 1 cut(s) 737
Psp6I CCWGG 1 cut(s) 1056
PspGI CCWGG 1 cut(s) 1056
PspN4I GGNNCC 3 cut(s) 182, 234, 461
PspPI GGNCC 5 cut(s) 217, 276, 285, 776, 1054
PstI CTGCAG 1 cut(s) 580
PstNI CAGNNNCTG 2 cut(s) 44, 410
PsuI RGATCY 2 cut(s) 232, 784
PvuII CAGCTG 2 cut(s) 41, 407
RsaI GTAC 2 cut(s) 361, 865
RsaNI GTAC 2 cut(s) 360, 864
RseI CAYNNNNRTG 3 cut(s) 513, 625, 833
SacI GAGCTC 1 cut(s) 737
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 1 cut(s) 822
SatI GCNGC 7 cut(s) 42, 45, 344, 405, 408, 411, 579
Sau3AI GATC 4 cut(s) 121, 232, 784, 1015
Sau96I GGNCC 5 cut(s) 217, 276, 285, 776, 1054
SchI GAGTC 4 cut(s) 421, 812, 857, 1090
ScrFI CCNGG 1 cut(s) 1058
SduI GDGCHC 2 cut(s) 520, 737
SfaNI GCATC 4 cut(s) 61, 177, 358, 422
SfcI CTRYAG 4 cut(s) 88, 261, 576, 1131
SinI GGWCC 3 cut(s) 276, 776, 1054
SmiMI CAYNNNNRTG 3 cut(s) 513, 625, 833
SmlI CTYRAG 3 cut(s) 101, 254, 983
SmoI CTYRAG 3 cut(s) 101, 254, 983
Sse9I AATT 8 cut(s) 29, 248, 331, 662, 714, 818, 969, 1147
SseBI AGGCCT 2 cut(s) 685, 1114
SsiI CCGC 1 cut(s) 457
SspMI CTAG 2 cut(s) 681, 957
SstI GAGCTC 1 cut(s) 737
StuI AGGCCT 2 cut(s) 685, 1114
StyD4I CCNGG 1 cut(s) 1056
TaqI TCGA 1 cut(s) 967
TaqII GACCGA 1 cut(s) 484
TasI AATT 8 cut(s) 29, 248, 331, 662, 714, 818, 969, 1147
TatI WGTACW 1 cut(s) 863
TfiI GAWTC 2 cut(s) 617, 951
Tru1I TTAA 1 cut(s) 822
Tru9I TTAA 1 cut(s) 822
TscAI CASTG 3 cut(s) 135, 170, 520
TseFI GTSAC 2 cut(s) 743, 1117
TseI GCWGC 7 cut(s) 41, 44, 343, 404, 407, 410, 578
Tsp45I GTSAC 2 cut(s) 743, 1117
TspDTI ATGAA 6 cut(s) 36, 305, 379, 630, 675, 851
TspGWI ACGGA 3 cut(s) 20, 920, 1067
TspRI CASTG 3 cut(s) 135, 170, 520
Van91I CCANNNNNTGG 1 cut(s) 400
VneI GTGCAC 1 cut(s) 516
VpaK11BI GGWCC 3 cut(s) 276, 776, 1054
XapI RAATTY 3 cut(s) 662, 714, 818
XceI RCATGY 1 cut(s) 78
XspI CTAG 2 cut(s) 681, 957
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.