Rorug03G0159300

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004240
Physical Location & Seq
Forward (+)
13210501 .. 13211132
632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0159300.1

Sequence Viewer

Length: 492 bp
ATGCTCCAACAGACCATTATTGACCTAGCACTAGCAGCTTCCCGTGCCATTCTCCCATCCAACTCCGGCATGTTCTATGTCCAACTAGGCATAGACACTCCGGCTATGCAAGCCAGCACCGCTTGTGATACCGTAAGCCCCATGACCCGGTTACCGGTTCCAGAGTGCGAGCTTTGCTTTCCTGTGGACGAGGGAGGGTCGACCCTCGAACCCAATCACTCTTCTGCGTTTACACTATTGAGCTCTGGCGACCCGATGTGCTCTCCTCCCCTCGGCATAAGCATTCCATTGTCCAACGGCTCGTGCGACTACAACCTTGGATATAGCAAGGGCTTTTTCGGCACGGACACACTCTCATACTCTGTTGATGATGCTGCTGCTTTCGAGGTTTCACTCAACTTCTCTATCGGTTGCGGCTTATTCAATCAATTGGCTTTCGGTGGTGACGACGAATCAGGGAATCCCATCTCCGGTGTGATGGGATGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

163

Amino Acids

17.01

Weight (kDa)

4.05

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 25 - 143 1.2e-09 Xylanase inhibitor N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 200
AciI CCGC 2 cut(s) 120, 414
AfiI CCNNNNNNNGG 4 cut(s) 147, 154, 272, 470
AgeI ACCGGT 1 cut(s) 154
AgsI TTSAA 1 cut(s) 424
AluBI AGCT 3 cut(s) 38, 172, 243
AluI AGCT 3 cut(s) 38, 172, 243
Alw21I GWGCWC 2 cut(s) 245, 263
ApeKI GCWGC 3 cut(s) 35, 374, 377
AsiGI ACCGGT 1 cut(s) 154
AsuC2I CCSGG 1 cut(s) 148
AsuHPI GGTGA 1 cut(s) 455
BanII GRGCYC 1 cut(s) 245
BauI CACGAG 1 cut(s) 301
Bbv12I GWGCWC 2 cut(s) 245, 263
BbvI GCAGC 3 cut(s) 47, 361, 364
BccI CCATC 4 cut(s) 64, 472, 473, 477
BceAI ACGGC 1 cut(s) 313
BcnI CCSGG 1 cut(s) 148
BfaI CTAG 3 cut(s) 26, 32, 86
BisI GCNGC 4 cut(s) 36, 375, 378, 415
BlsI GCNGC 4 cut(s) 37, 376, 379, 416
Bme1390I CCNGG 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 1 cut(s) 148
BmsI GCATC 1 cut(s) 361
BpuMI CCSGG 1 cut(s) 148
BsaJI CCNNGG 2 cut(s) 271, 316
BsaWI WCCGGW 2 cut(s) 154, 470
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 154, 272, 470
Bse118I RCCGGY 1 cut(s) 154
BseDI CCNNGG 2 cut(s) 271, 316
BseGI GGATG 2 cut(s) 56, 488
BseLI CCNNNNNNNGG 4 cut(s) 147, 154, 272, 470
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 3 cut(s) 47, 361, 364
BshTI ACCGGT 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 245, 263
BsiSI CCGG 5 cut(s) 66, 101, 148, 155, 471
BslI CCNNNNNNNGG 4 cut(s) 147, 154, 272, 470
BsmI GAATGC 1 cut(s) 282
Bsp1286I GDGCHC 2 cut(s) 245, 263
BspACI CCGC 2 cut(s) 120, 414
BspLI GGNNCC 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 154
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 2 cut(s) 271, 316
BssSI CACGAG 1 cut(s) 301
BssT1I CCWWGG 1 cut(s) 316
Bst2BI CACGAG 1 cut(s) 301
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 1 cut(s) 226
BstC8I GCNNGC 3 cut(s) 111, 115, 170
BstEII GGTNACC 1 cut(s) 150
BstF5I GGATG 2 cut(s) 56, 488
BstMWI GCNNNNNNNGC 6 cut(s) 35, 44, 110, 119, 174, 339
BstNSI RCATGY 1 cut(s) 73
BstPI GGTNACC 1 cut(s) 150
BstSCI CCNGG 1 cut(s) 146
BstV1I GCAGC 3 cut(s) 47, 361, 364
BtsCI GGATG 2 cut(s) 56, 488
Cac8I GCNNGC 3 cut(s) 111, 115, 170
Cfr10I RCCGGY 1 cut(s) 154
CspAI ACCGGT 1 cut(s) 154
CviAII CATG 2 cut(s) 70, 142
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 243
Eco91I GGTNACC 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 243
EcoO65I GGTNACC 1 cut(s) 150
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 2 cut(s) 73, 145
FaiI YATR 8 cut(s) 71, 78, 92, 107, 143, 278, 324, 358
FatI CATG 2 cut(s) 69, 141
FblI GTMKAC 1 cut(s) 200
Fnu4HI GCNGC 4 cut(s) 36, 375, 378, 415
FokI GGATG 1 cut(s) 43
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 4 cut(s) 36, 375, 378, 415
FspBI CTAG 3 cut(s) 26, 32, 86
GluI GCNGC 4 cut(s) 36, 375, 378, 415
HapII CCGG 5 cut(s) 66, 101, 148, 155, 471
Hin1II CATG 2 cut(s) 73, 145
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HinfI GANTC 2 cut(s) 452, 460
HpaII CCGG 5 cut(s) 66, 101, 148, 155, 471
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 3 cut(s) 187, 201, 231
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 3 cut(s) 187, 201, 231
Hpy99I CGWCG 1 cut(s) 452
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4V TGCA 1 cut(s) 109
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 44, 110, 119, 174, 339
Hsp92II CATG 2 cut(s) 73, 145
LmnI GCTCC 1 cut(s) 9
Lsp1109I GCAGC 3 cut(s) 47, 361, 364
LweI GCATC 1 cut(s) 361
MaeI CTAG 3 cut(s) 26, 32, 86
MaeIII GTNAC 2 cut(s) 150, 443
MboII GAAGA 1 cut(s) 213
MfeI CAATTG 1 cut(s) 428
MhlI GDGCHC 2 cut(s) 245, 263
MluCI AATT 1 cut(s) 428
MmeI TCCRAC 4 cut(s) 31, 84, 106, 318
MnlI CCTC 6 cut(s) 184, 188, 215, 276, 281, 379
MspI CCGG 5 cut(s) 66, 101, 148, 155, 471
MspR9I CCNGG 1 cut(s) 148
MunI CAATTG 1 cut(s) 428
Mva1269I GAATGC 1 cut(s) 282
MwoI GCNNNNNNNGC 6 cut(s) 35, 44, 110, 119, 174, 339
NciI CCSGG 1 cut(s) 148
NlaIII CATG 2 cut(s) 73, 145
NlaIV GGNNCC 1 cut(s) 159
NmeAIII GCCGAG 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 443
NspI RCATGY 1 cut(s) 73
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 444
PctI GAATGC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 452, 460
PinAI ACCGGT 1 cut(s) 154
PkrI GCNGC 4 cut(s) 37, 376, 379, 416
Psp124BI GAGCTC 1 cut(s) 245
PspEI GGTNACC 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 159
SacI GAGCTC 1 cut(s) 245
SalI GTCGAC 1 cut(s) 199
SatI GCNGC 4 cut(s) 36, 375, 378, 415
ScrFI CCNGG 1 cut(s) 148
SduI GDGCHC 2 cut(s) 245, 263
SetI ASST 6 cut(s) 27, 40, 174, 245, 318, 390
SfaNI GCATC 1 cut(s) 361
Sse9I AATT 1 cut(s) 428
SsiI CCGC 2 cut(s) 120, 414
SspMI CTAG 3 cut(s) 26, 32, 86
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 146
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 1 cut(s) 133
TaqI TCGA 3 cut(s) 200, 207, 384
TasI AATT 1 cut(s) 428
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 2 cut(s) 452, 460
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 3 cut(s) 35, 374, 377
Tsp45I GTSAC 1 cut(s) 443
TspGWI ACGGA 1 cut(s) 359
XceI RCATGY 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 200
XspI CTAG 3 cut(s) 26, 32, 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.