MD14G1043800.v1.1

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004240
Physical Location & Seq
Reverse (-)
4120861 .. 4126268
5408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1043800.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGGAGATTCCAAATAAAATGGAGGCAAAGGATGCTGAAAGTCATATGATATCAGCTGCTGCATTTGTGGAAGGGGGAATACAAGAGGATTATGGCGAAGCCTGCAGCATATGCCTTGATGAGTTTTGTGACAGTGATCCATCAGTGGTTACTTCTTGCAAGCATGAGTTTCACCTCCACTGCATCCTTGAGTGGTGCCAGCGAAGCTCTCAATGTCCCATGTGTTGGCAACCCATTAGCTTGAAGGATCCTTGCAGTCAAGAATTACTGGAGGGAGTAGAACGGGAGAGAAGATTTAGGGAGACAGCAAGAAATGCAACTATATTTCATCATCCCACCCTTGGCGATTTTCAATTTCAGCATTTACCTGTGGGTACTACCAATGCTGAACTTGAAGAGCGCATCATTCAGCACTTGGCCGCTGCTGCTTCTATGGGAAGGGCCCATCACCATGGTAGAAGGGAAAGCCATCGGAGCCGATCATCTGCTCGTGGTCATCCACACTTCTCGGTACCTTCTACACATCCTAGTGCATCTCCTCTTGGTCCTGTTCATGCCCCAGGAGGGGACGGTGAACCAGCTGAAATAACTGTAGCAAGTCCATCTACCCCACTTACTTCAGATGTGGATGAATCATCACAAAGGAGTGCACAATATCCTTCTGTTCAGACTGATGGAATTTCTTCCTCACCATCTGGGTCTTCACGCATGCATAGAAATCGTCAAGGAAATTCCTCTAGTCATTGCTCTACTAGCCATGCCTCATCGTTGGATCAGGACAGAGCAGGACCATCAGAACTTCACTCTCTTTCAGAGTCTCTGAAATCTAAGTTTAATTCCATGTCAATGAGGTACAAGGAATCATTTTCGAGGAGTACACGAGGATTGAAGGAGAGGCTGTTCTCCCGTAGCCCTTCTATGTCGGAACTCAGTTCTGAAGTTCGTAGAGAGGTTAATGCTGGAATTGCCACCGTATCTCGAATGATGGAGCGCCTTGAGACAAGAGACAATAGTACAGACAACCAGTCTTCTGTAGTGAATCAGAATCCAGATGGTTCTGCTGCAGAGCAGAGGAACCAGAACCGTGCAGAGATCCGTGGAGAAAACCCTTTGAGCGACAACAATAGGCCTGGTACTACTTGTGCCGCAGGTTCCACTTCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

389

Amino Acids

42.55

Weight (kDa)

6.09

Isoelectric Point (pI)

57.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 31 - 75 1.4e-08 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 31 - 82 2.1e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 34 - 75 9.1e-10 Ring finger domain
zf-C3HC4_2 PF13923 35 - 75 4.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 35 - 62 7.6e-07 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1139
Acc65I GGTACC 1 cut(s) 511
AccB1I GGYRCC 2 cut(s) 195, 511
AccB7I CCANNNNNTGG 1 cut(s) 225
AciI CCGC 2 cut(s) 420, 1146
AclWI GGATC 5 cut(s) 131, 242, 255, 780, 1087
AcoI YGGCCR 1 cut(s) 417
AcsI RAATTY 2 cut(s) 678, 730
AcuI CTGAAG 2 cut(s) 603, 957
AfaI GTAC 6 cut(s) 376, 513, 854, 877, 1015, 1135
AfiI CCNNNNNNNGG 4 cut(s) 225, 341, 564, 565
AgsI TTSAA 4 cut(s) 244, 353, 395, 889
AhdI GACNNNNNGTC 1 cut(s) 1024
AjnI CCWGG 2 cut(s) 559, 1129
AloI GAACNNNNNNTCC 2 cut(s) 884, 916
AluBI AGCT 4 cut(s) 56, 207, 240, 581
AluI AGCT 4 cut(s) 56, 207, 240, 581
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 4 cut(s) 296, 822, 992, 999
Alw44I GTGCAC 1 cut(s) 648
AlwI GGATC 5 cut(s) 131, 242, 255, 780, 1087
AlwNI CAGNNNCTG 1 cut(s) 59
AoxI GGCC 3 cut(s) 417, 441, 1127
ApaI GGGCCC 1 cut(s) 445
ApaLI GTGCAC 1 cut(s) 648
ApeKI GCWGC 6 cut(s) 56, 59, 105, 422, 425, 1061
ApoI RAATTY 2 cut(s) 678, 730
Asp700I GAANNNNTTC 1 cut(s) 682
Asp718I GGTACC 1 cut(s) 511
AspLEI GCGC 2 cut(s) 402, 993
AspS9I GGNCC 4 cut(s) 441, 442, 545, 788
AsuHPI GGTGA 4 cut(s) 164, 440, 584, 681
AvaII GGWCC 2 cut(s) 545, 788
BaeGI GKGCMC 2 cut(s) 445, 652
BamHI GGATCC 1 cut(s) 247
BanI GGYRCC 2 cut(s) 195, 511
BanII GRGCYC 1 cut(s) 445
BarI GAAGNNNNNNTAC 2 cut(s) 63, 95
BauI CACGAG 2 cut(s) 489, 879
BbsI GAAGAC 2 cut(s) 693, 1020
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 6 cut(s) 43, 46, 117, 409, 412, 1048
BccI CCATC 9 cut(s) 148, 453, 477, 610, 668, 700, 799, 979, 1046
BcgI CGANNNNNNTGC 2 cut(s) 701, 735
BciT130I CCWGG 2 cut(s) 561, 1131
BcoDI GTCTC 4 cut(s) 296, 822, 992, 999
BfaI CTAG 3 cut(s) 528, 738, 753
BfmI CTRYAG 4 cut(s) 103, 591, 1032, 1062
BfoI RGCGCY 1 cut(s) 994
BfuAI ACCTGC 1 cut(s) 1139
BisI GCNGC 8 cut(s) 57, 60, 106, 420, 423, 426, 1062, 1146
BlsI GCNGC 8 cut(s) 58, 61, 107, 421, 424, 427, 1063, 1147
Bme1390I CCNGG 2 cut(s) 561, 1131
Bme18I GGWCC 2 cut(s) 545, 788
BmeRI GACNNNNNGTC 1 cut(s) 1024
BmgT120I GGNCC 4 cut(s) 441, 442, 545, 788
BmiI GGNNCC 7 cut(s) 197, 249, 443, 476, 513, 1076, 1153
BmrFI CCNGG 2 cut(s) 561, 1131
BmsI GCATC 4 cut(s) 22, 192, 411, 542
BpiI GAAGAC 2 cut(s) 693, 1020
BpmI CTGGAG 1 cut(s) 290
BpuEI CTTGAG 2 cut(s) 209, 1016
BsaJI CCNNGG 4 cut(s) 340, 451, 559, 1096
BsaXI ACNNNNNCTCC 2 cut(s) 884, 914
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 341, 564, 565
Bse1I ACTGG 2 cut(s) 273, 1024
Bse3DI GCAATG 1 cut(s) 742
BseBI CCWGG 2 cut(s) 561, 1131
BseDI CCNNGG 4 cut(s) 340, 451, 559, 1096
BseGI GGATG 6 cut(s) 37, 183, 331, 496, 523, 634
BseLI CCNNNNNNNGG 4 cut(s) 225, 341, 564, 565
BseMI GCAATG 1 cut(s) 742
BseMII CTCAG 1 cut(s) 943
BseNI ACTGG 2 cut(s) 273, 1024
BseRI GAGGAG 2 cut(s) 528, 886
BseSI GKGCMC 2 cut(s) 445, 652
BseXI GCAGC 6 cut(s) 43, 46, 117, 409, 412, 1048
BsgI GTGCAG 1 cut(s) 1107
BshFI GGCC 3 cut(s) 419, 443, 1129
BshNI GGYRCC 2 cut(s) 195, 511
BsiHKAI GWGCWC 1 cut(s) 652
BslFI GGGAC 2 cut(s) 201, 581
BslI CCNNNNNNNGG 4 cut(s) 225, 341, 564, 565
BsmAI GTCTC 4 cut(s) 296, 822, 992, 999
BsmFI GGGAC 2 cut(s) 201, 581
BsnI GGCC 3 cut(s) 419, 443, 1129
Bsp120I GGGCCC 1 cut(s) 441
Bsp1286I GDGCHC 2 cut(s) 445, 652
Bsp143I GATC 5 cut(s) 136, 247, 479, 772, 1092
Bsp19I CCATGG 1 cut(s) 451
BspACI CCGC 2 cut(s) 420, 1146
BspANI GGCC 3 cut(s) 419, 443, 1129
BspCNI CTCAG 1 cut(s) 942
BspLI GGNNCC 7 cut(s) 197, 249, 443, 476, 513, 1076, 1153
BspMAI CTGCAG 2 cut(s) 107, 1066
BspMI ACCTGC 1 cut(s) 1139
BspPI GGATC 5 cut(s) 131, 242, 255, 780, 1087
BspQI GCTCTTC 1 cut(s) 390
BspT107I GGYRCC 2 cut(s) 195, 511
BsrDI GCAATG 1 cut(s) 742
BsrI ACTGG 2 cut(s) 273, 1024
BssECI CCNNGG 4 cut(s) 340, 451, 559, 1096
BssMI GATC 5 cut(s) 136, 247, 479, 772, 1092
BssSI CACGAG 2 cut(s) 489, 879
BssT1I CCWWGG 2 cut(s) 340, 451
Bst2BI CACGAG 2 cut(s) 489, 879
Bst2UI CCWGG 2 cut(s) 561, 1131
Bst4CI ACNGT 5 cut(s) 134, 572, 592, 973, 1085
Bst6I CTCTTC 1 cut(s) 390
BstAPI GCANNNNNTGC 3 cut(s) 32, 111, 314
BstC8I GCNNGC 4 cut(s) 103, 161, 200, 710
BstDEI CTNAG 2 cut(s) 828, 929
BstDSI CCRYGG 2 cut(s) 451, 1096
BstF5I GGATG 6 cut(s) 37, 183, 331, 496, 523, 634
BstH2I RGCGCY 1 cut(s) 994
BstHHI GCGC 2 cut(s) 402, 993
BstKTI GATC 5 cut(s) 139, 250, 482, 775, 1095
BstMAI GTCTC 4 cut(s) 296, 822, 992, 999
BstMBI GATC 5 cut(s) 136, 247, 479, 772, 1092
BstMWI GCNNNNNNNGC 9 cut(s) 32, 102, 111, 204, 314, 425, 474, 753, 965
BstNI CCWGG 2 cut(s) 561, 1131
BstNSI RCATGY 1 cut(s) 712
BstSCI CCNGG 2 cut(s) 559, 1129
BstSFI CTRYAG 4 cut(s) 103, 591, 1032, 1062
BstSLI GKGCMC 2 cut(s) 445, 652
BstV1I GCAGC 6 cut(s) 43, 46, 117, 409, 412, 1048
BstV2I GAAGAC 2 cut(s) 693, 1020
BstX2I RGATCY 2 cut(s) 247, 1092
BstXI CCANNNNNNTGG 1 cut(s) 452
BstYI RGATCY 2 cut(s) 247, 1092
BsuRI GGCC 3 cut(s) 419, 443, 1129
BtgI CCRYGG 2 cut(s) 451, 1096
BtsCI GGATG 6 cut(s) 37, 183, 331, 496, 523, 634
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 3 cut(s) 139, 150, 178
BveI ACCTGC 1 cut(s) 1139
Cac8I GCNNGC 4 cut(s) 103, 161, 200, 710
CaiI CAGNNNCTG 1 cut(s) 59
CfoI GCGC 2 cut(s) 402, 993
Cfr13I GGNCC 4 cut(s) 441, 442, 545, 788
Csp6I GTAC 6 cut(s) 375, 512, 853, 876, 1014, 1134
CviAII CATG 7 cut(s) 164, 220, 452, 554, 709, 758, 841
CviQI GTAC 6 cut(s) 375, 512, 853, 876, 1014, 1134
DdeI CTNAG 2 cut(s) 828, 929
DpnI GATC 5 cut(s) 138, 249, 481, 774, 1094
DpnII GATC 5 cut(s) 136, 247, 479, 772, 1092
DriI GACNNNNNGTC 1 cut(s) 1024
EaeI YGGCCR 1 cut(s) 417
Eam1104I CTCTTC 1 cut(s) 390
Eam1105I GACNNNNNGTC 1 cut(s) 1024
EarI CTCTTC 1 cut(s) 390
Eco130I CCWWGG 2 cut(s) 340, 451
Eco147I AGGCCT 1 cut(s) 1129
Eco24I GRGCYC 1 cut(s) 445
Eco32I GATATC 1 cut(s) 51
Eco47I GGWCC 2 cut(s) 545, 788
Eco57I CTGAAG 2 cut(s) 603, 957
EcoO109I RGGNCCY 1 cut(s) 441
EcoRII CCWGG 2 cut(s) 559, 1129
EcoRV GATATC 1 cut(s) 51
EcoT14I CCWWGG 2 cut(s) 340, 451
EcoT22I ATGCAT 1 cut(s) 714
EcoT38I GRGCYC 1 cut(s) 445
ErhI CCWWGG 2 cut(s) 340, 451
FaeI CATG 7 cut(s) 167, 223, 455, 557, 712, 761, 844
FaqI GGGAC 2 cut(s) 201, 581
FatI CATG 7 cut(s) 163, 219, 451, 553, 708, 757, 840
FauNDI CATATG 2 cut(s) 45, 110
Fnu4HI GCNGC 8 cut(s) 57, 60, 106, 420, 423, 426, 1062, 1146
FokI GGATG 6 cut(s) 44, 170, 318, 483, 510, 641
FriOI GRGCYC 1 cut(s) 445
Fsp4HI GCNGC 8 cut(s) 57, 60, 106, 420, 423, 426, 1062, 1146
FspBI CTAG 3 cut(s) 528, 738, 753
GlaI GCGC 2 cut(s) 401, 992
GluI GCNGC 8 cut(s) 57, 60, 106, 420, 423, 426, 1062, 1146
GsuI CTGGAG 1 cut(s) 290
HaeII RGCGCY 1 cut(s) 994
HaeIII GGCC 3 cut(s) 419, 443, 1129
HhaI GCGC 2 cut(s) 402, 993
Hin1II CATG 7 cut(s) 167, 223, 455, 557, 712, 761, 844
Hin6I GCGC 2 cut(s) 400, 991
HinP1I GCGC 2 cut(s) 400, 991
HinfI GANTC 6 cut(s) 7, 632, 815, 860, 1039, 1045
HphI GGTGA 4 cut(s) 164, 440, 584, 681
Hpy166II GTNNAC 3 cut(s) 575, 650, 878
Hpy188I TCNGA 9 cut(s) 474, 622, 669, 796, 814, 822, 925, 937, 1044
Hpy188III TCNNGA 4 cut(s) 260, 776, 978, 1049
Hpy8I GTNNAC 3 cut(s) 575, 650, 878
HpyAV CCTTC 8 cut(s) 65, 238, 432, 453, 525, 669, 883, 924
HpyCH4III ACNGT 5 cut(s) 134, 572, 592, 973, 1085
HpyF10VI GCNNNNNNNGC 9 cut(s) 32, 102, 111, 204, 314, 425, 474, 753, 965
HpyF3I CTNAG 2 cut(s) 828, 929
Hsp92II CATG 7 cut(s) 167, 223, 455, 557, 712, 761, 844
HspAI GCGC 2 cut(s) 400, 991
KpnI GGTACC 1 cut(s) 515
Kzo9I GATC 5 cut(s) 136, 247, 479, 772, 1092
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 2 cut(s) 474, 988
Lsp1109I GCAGC 6 cut(s) 43, 46, 117, 409, 412, 1048
LweI GCATC 4 cut(s) 22, 192, 411, 542
MaeI CTAG 3 cut(s) 528, 738, 753
MaeIII GTNAC 2 cut(s) 128, 148
MalI GATC 5 cut(s) 138, 249, 481, 774, 1094
MboI GATC 5 cut(s) 136, 247, 479, 772, 1092
MboII GAAGA 5 cut(s) 303, 407, 675, 693, 1020
MflI RGATCY 2 cut(s) 247, 1092
MhlI GDGCHC 2 cut(s) 445, 652
MluCI AATT 6 cut(s) 263, 353, 678, 730, 835, 963
MlyI GAGTC 1 cut(s) 824
MmeI TCCRAC 2 cut(s) 750, 903
Mph1103I ATGCAT 1 cut(s) 714
MroXI GAANNNNTTC 1 cut(s) 682
MseI TTAA 2 cut(s) 834, 954
MslI CAYNNNNRTG 3 cut(s) 450, 528, 845
MspA1I CMGCKG 3 cut(s) 56, 422, 581
MspR9I CCNGG 2 cut(s) 561, 1131
MvaI CCWGG 2 cut(s) 561, 1131
MwoI GCNNNNNNNGC 9 cut(s) 32, 102, 111, 204, 314, 425, 474, 753, 965
NcoI CCATGG 1 cut(s) 451
NdeI CATATG 2 cut(s) 45, 110
NdeII GATC 5 cut(s) 136, 247, 479, 772, 1092
NlaIII CATG 7 cut(s) 167, 223, 455, 557, 712, 761, 844
NlaIV GGNNCC 7 cut(s) 197, 249, 443, 476, 513, 1076, 1153
NmuCI GTSAC 1 cut(s) 128
NsiI ATGCAT 1 cut(s) 714
NspI RCATGY 1 cut(s) 712
PaeI GCATGC 1 cut(s) 712
PceI AGGCCT 1 cut(s) 1129
PciSI GCTCTTC 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 682
PfeI GAWTC 5 cut(s) 7, 632, 860, 1039, 1045
PflMI CCANNNNNTGG 1 cut(s) 225
PkrI GCNGC 8 cut(s) 58, 61, 107, 421, 424, 427, 1063, 1147
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
Psp6I CCWGG 2 cut(s) 559, 1129
PspGI CCWGG 2 cut(s) 559, 1129
PspN4I GGNNCC 7 cut(s) 197, 249, 443, 476, 513, 1076, 1153
PspOMI GGGCCC 1 cut(s) 441
PspPI GGNCC 4 cut(s) 441, 442, 545, 788
PstI CTGCAG 2 cut(s) 107, 1066
PstNI CAGNNNCTG 1 cut(s) 59
PsuI RGATCY 2 cut(s) 247, 1092
PvuII CAGCTG 2 cut(s) 56, 581
RsaI GTAC 6 cut(s) 376, 513, 854, 877, 1015, 1135
RsaNI GTAC 6 cut(s) 375, 512, 853, 876, 1014, 1134
RseI CAYNNNNRTG 3 cut(s) 450, 528, 845
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 2 cut(s) 834, 954
SatI GCNGC 8 cut(s) 57, 60, 106, 420, 423, 426, 1062, 1146
Sau3AI GATC 5 cut(s) 136, 247, 479, 772, 1092
Sau96I GGNCC 4 cut(s) 441, 442, 545, 788
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 2 cut(s) 561, 1131
SduI GDGCHC 2 cut(s) 445, 652
SfaNI GCATC 4 cut(s) 22, 192, 411, 542
SfcI CTRYAG 4 cut(s) 103, 591, 1032, 1062
SinI GGWCC 2 cut(s) 545, 788
SmiMI CAYNNNNRTG 3 cut(s) 450, 528, 845
SmlI CTYRAG 2 cut(s) 188, 995
SmoI CTYRAG 2 cut(s) 188, 995
SphI GCATGC 1 cut(s) 712
Sse9I AATT 6 cut(s) 263, 353, 678, 730, 835, 963
SseBI AGGCCT 1 cut(s) 1129
SsiI CCGC 2 cut(s) 420, 1146
SspMI CTAG 3 cut(s) 528, 738, 753
StuI AGGCCT 1 cut(s) 1129
StyD4I CCNGG 2 cut(s) 559, 1129
StyI CCWWGG 2 cut(s) 340, 451
TaaI ACNGT 5 cut(s) 134, 572, 592, 973, 1085
TaqI TCGA 2 cut(s) 869, 979
TasI AATT 6 cut(s) 263, 353, 678, 730, 835, 963
TatI WGTACW 2 cut(s) 875, 1013
TauI GCSGC 2 cut(s) 422, 1148
TfiI GAWTC 5 cut(s) 7, 632, 860, 1039, 1045
Tru1I TTAA 2 cut(s) 834, 954
Tru9I TTAA 2 cut(s) 834, 954
TscAI CASTG 3 cut(s) 139, 150, 185
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 6 cut(s) 56, 59, 105, 422, 425, 1061
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 3 cut(s) 317, 542, 645
TspGWI ACGGA 1 cut(s) 1085
TspRI CASTG 3 cut(s) 139, 150, 185
Van91I CCANNNNNTGG 1 cut(s) 225
VneI GTGCAC 1 cut(s) 648
VpaK11BI GGWCC 2 cut(s) 545, 788
XapI RAATTY 2 cut(s) 678, 730
XceI RCATGY 1 cut(s) 712
XmnI GAANNNNTTC 1 cut(s) 682
XspI CTAG 3 cut(s) 528, 738, 753
Zsp2I ATGCAT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.