Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0004240

Physical Location & Seq

Genomic Coordinates Reverse (-)

3733955 .. 3736682

Gene Structure 2728 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g03880.8

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1182 bp

ATGCGACTTTTCACAAACCATATTCCAGATAAAATGGAGGCAGAGGCTGAAAGTCATATGATATCAGCTGCTGCATTTGTGGAAGGGGGAATACAAGAGGCTTATGGCGAGGCCTGCAGCATATGCCTTGAGGAGTTTTGTGATGGTGATCCTTCAGTGGTTACTTGTTGCAAGCATGAGTTTCACCTCCACTGCATCCTTGAGTGGTGCCAGCGAAGCTCTCAATGTCCCATGTGTTGGCAACCCATTAGCTTGAAGGATCCTTGCAGCCAAGAATTACTGGAGGGAGTAGAAAGGGAGAGAAGATTTAGGGAGACAGCAAGAAATGCACCCATATTTCATCATCCCACCCTTGGCGATTTTCAATTTCAGCATTTACCTGTGGGTACTACCAATGCTGAACTTGAAGAGCGCATAATTCAGCACTTGGCTGCTGCTGCTTCTATGGGAAGGGCCCACCACCATGGTAGAAGGGAAGGCCATAGGAGCCGATCATCTGCTCGTGGTCATCCACACATCTCAGTACCTTCTACTCATTCTAGGTCATCTCCTCTTGGTCCTGTTCATGCCCCCGGAGGAGACAGTGAACCAGCTGAAATAACTGTAGCAAGTCCATCTACCCCACTTACATCAGATGGAGACGAAACATCACGAATGAGCACAACATATCCTTCTGTTCAGACAGATGGAATTTCTTCCTTGCCATCTGGGTCTTCAAGCATGCATAGAAATCGTCAAGGAAATTCCTCTAGCAATTTGAGCTCTACTAGCCATGCCTCCTCGTTGAATCAGGACAGAGCAGGACCATCAGAACTTCACTCTCTTTCAGAGTCTCTGAAATCTAAGTTTAATTCTATGTCAATGAGGTACAAGGAATCGTTTTCGAGGAGTACACGAGGATTGAAGGAGAGGCTGTTCTCTCGTAGCACTTCTATGTCGGAACTCAGTTCTGAAGTCCGTAGAGAGGTTAATGCTGGAATTGCCACCGTATCTCGAATGATGGAGCGCCTTGAGACAAGAGATAATAGTACGGACAACCAGTCTTCTGGAGTGAATCAGACTGCAGATGATTCTGCTGCAGAGCAGAACAACCAGAACCGTGCAGGGATTCGTGGAGAAAACCCTTTGAGTGACAACAATATGCCTGGTACTACATGTGCCACAGGTTCTGCTTCACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

394

Amino Acids

42.92

Weight (kDa)

6.09

Isoelectric Point (pI)

56.44

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004240)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g20900 FvH4_6g20900 FvH4_6g20900 FvH4_6g20900 FvH4_6g20900 FvH4_6g20900 FvH4_6g20900
malus_domestica	MD12G1045000.v1.1 MD14G1043800.v1.1
prunus_persica	Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1
pyrus_communis	pycom12g03880 pycom14g03720
rosa_chinensis	RchiOBHm_Chr3g0477251
rosa_laevigata	RLG00000023734
rosa_multiflora	Rmu_co8521747.1_g000001 Rmu_sc0000348.1_g000004
rosa_roxburghii	Rroxscaffold_6G00404290 Rroxscaffold_6G00404770
rosa_rugosa	Rorug03G0159000.1 Rorug03G0159100 Rorug03G0159200 Rorug03G0159300
rosa_samantha	Rh3AG209600 Rh3BG243000 Rh3CG237100 Rh3DG236200
rosa_wichuraiana	Rw0G000250 Rw0G021740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	207
AccB7I	CCANNNNNTGG	1 cut(s)	237
AclWI	GGATC	3 cut(s)	143, 254, 267
AcsI	RAATTY	2 cut(s)	690, 742
AcuI	CTGAAG	2 cut(s)	138, 972
AfaI	GTAC	6 cut(s)	388, 525, 869, 892, 1030, 1150
AfiI	CCNNNNNNNGG	3 cut(s)	237, 353, 964
AflIII	ACRYGT	1 cut(s)	1154
AgsI	TTSAA	6 cut(s)	256, 365, 407, 717, 787, 904
AhdI	GACNNNNNGTC	1 cut(s)	1039
AjnI	CCWGG	1 cut(s)	1144
AloI	GAACNNNNNNTCC	2 cut(s)	899, 931
AluBI	AGCT	5 cut(s)	68, 219, 252, 593, 762
AluI	AGCT	5 cut(s)	68, 219, 252, 593, 762
Alw21I	GWGCWC	2 cut(s)	662, 764
Alw26I	GTCTC	5 cut(s)	308, 573, 633, 837, 1007
AlwI	GGATC	3 cut(s)	143, 254, 267
AlwNI	CAGNNNCTG	3 cut(s)	47, 71, 1169
AoxI	GGCC	3 cut(s)	111, 453, 478
ApaI	GGGCCC	1 cut(s)	457
ApeKI	GCWGC	8 cut(s)	68, 71, 117, 267, 431, 434, 437, 1076
ApoI	RAATTY	2 cut(s)	690, 742
Asp700I	GAANNNNTTC	1 cut(s)	694
AspLEI	GCGC	2 cut(s)	414, 1008
AspS9I	GGNCC	4 cut(s)	453, 454, 557, 803
AsuC2I	CCSGG	1 cut(s)	573
AsuHPI	GGTGA	2 cut(s)	158, 176
AvaII	GGWCC	2 cut(s)	557, 803
BaeGI	GKGCMC	1 cut(s)	457
BamHI	GGATCC	1 cut(s)	259
BanI	GGYRCC	1 cut(s)	207
BanII	GRGCYC	2 cut(s)	457, 764
BarI	GAAGNNNNNNTAC	2 cut(s)	75, 107
BauI	CACGAG	2 cut(s)	501, 894
BbsI	GAAGAC	2 cut(s)	705, 1035
Bbv12I	GWGCWC	2 cut(s)	662, 764
BbvI	GCAGC	8 cut(s)	55, 58, 129, 279, 418, 421, 424, 1063
BccI	CCATC	7 cut(s)	137, 622, 629, 680, 712, 814, 994
BcgI	CGANNNNNNTGC	2 cut(s)	713, 747
BciT130I	CCWGG	1 cut(s)	1146
BcnI	CCSGG	1 cut(s)	573
BcoDI	GTCTC	5 cut(s)	308, 573, 633, 837, 1007
BfaI	CTAG	3 cut(s)	540, 750, 768
BfmI	CTRYAG	4 cut(s)	115, 603, 1062, 1077
BfoI	RGCGCY	1 cut(s)	1009
BisI	GCNGC	8 cut(s)	69, 72, 118, 268, 432, 435, 438, 1077
BlsI	GCNGC	8 cut(s)	70, 73, 119, 269, 433, 436, 439, 1078
Bme1390I	CCNGG	2 cut(s)	573, 1146
Bme18I	GGWCC	2 cut(s)	557, 803
BmeRI	GACNNNNNGTC	1 cut(s)	1039
BmgT120I	GGNCC	4 cut(s)	453, 454, 557, 803
BmiI	GGNNCC	4 cut(s)	209, 261, 455, 488
BmrFI	CCNGG	2 cut(s)	573, 1146
BmsI	GCATC	1 cut(s)	204
BpiI	GAAGAC	2 cut(s)	705, 1035
BpmI	CTGGAG	2 cut(s)	302, 1068
BpuEI	CTTGAG	3 cut(s)	149, 221, 1031
BpuMI	CCSGG	1 cut(s)	573
BsaBI	GATNNNNATC	1 cut(s)	147
BsaJI	CCNNGG	3 cut(s)	352, 463, 571
BsaXI	ACNNNNNCTCC	2 cut(s)	899, 929
Bsc4I	CCNNNNNNNGG	3 cut(s)	237, 353, 964
Bse1I	ACTGG	2 cut(s)	285, 1039
Bse8I	GATNNNNATC	1 cut(s)	147
BseBI	CCWGG	1 cut(s)	1146
BseDI	CCNNGG	3 cut(s)	352, 463, 571
BseGI	GGATG	3 cut(s)	195, 343, 508
BseJI	GATNNNNATC	1 cut(s)	147
BseLI	CCNNNNNNNGG	3 cut(s)	237, 353, 964
BseMII	CTCAG	2 cut(s)	534, 958
BseNI	ACTGG	2 cut(s)	285, 1039
BseRI	GAGGAG	5 cut(s)	146, 540, 591, 769, 901
BseSI	GKGCMC	1 cut(s)	457
BseXI	GCAGC	8 cut(s)	55, 58, 129, 279, 418, 421, 424, 1063
BsgI	GTGCAG	1 cut(s)	1122
BshFI	GGCC	3 cut(s)	113, 455, 480
BshNI	GGYRCC	1 cut(s)	207
BsiHKAI	GWGCWC	2 cut(s)	662, 764
BsiSI	CCGG	1 cut(s)	573
BslFI	GGGAC	1 cut(s)	213
BslI	CCNNNNNNNGG	3 cut(s)	237, 353, 964
BsmAI	GTCTC	5 cut(s)	308, 573, 633, 837, 1007
BsmBI	CGTCTC	1 cut(s)	633
BsmFI	GGGAC	1 cut(s)	213
BsnI	GGCC	3 cut(s)	113, 455, 480
Bsp120I	GGGCCC	1 cut(s)	453
Bsp1286I	GDGCHC	3 cut(s)	457, 662, 764
Bsp143I	GATC	3 cut(s)	148, 259, 491
Bsp19I	CCATGG	1 cut(s)	463
BspANI	GGCC	3 cut(s)	113, 455, 480
BspCNI	CTCAG	2 cut(s)	533, 957
BspLI	GGNNCC	4 cut(s)	209, 261, 455, 488
BspMAI	CTGCAG	3 cut(s)	119, 1066, 1081
BspPI	GGATC	3 cut(s)	143, 254, 267
BspQI	GCTCTTC	1 cut(s)	402
BspT107I	GGYRCC	1 cut(s)	207
BsrI	ACTGG	2 cut(s)	285, 1039
BssECI	CCNNGG	3 cut(s)	352, 463, 571
BssMI	GATC	3 cut(s)	148, 259, 491
BssSI	CACGAG	2 cut(s)	501, 894
BssT1I	CCWWGG	2 cut(s)	352, 463
Bst2BI	CACGAG	2 cut(s)	501, 894
Bst2UI	CCWGG	1 cut(s)	1146
Bst4CI	ACNGT	4 cut(s)	584, 604, 988, 1100
Bst6I	CTCTTC	1 cut(s)	402
BstAPI	GCANNNNNTGC	2 cut(s)	123, 326
BstC8I	GCNNGC	4 cut(s)	115, 173, 212, 722
BstDEI	CTNAG	3 cut(s)	520, 843, 944
BstDSI	CCRYGG	1 cut(s)	463
BstF5I	GGATG	3 cut(s)	195, 343, 508
BstH2I	RGCGCY	1 cut(s)	1009
BstHHI	GCGC	2 cut(s)	414, 1008
BstKTI	GATC	3 cut(s)	151, 262, 494
BstMAI	GTCTC	5 cut(s)	308, 573, 633, 837, 1007
BstMBI	GATC	3 cut(s)	148, 259, 491
BstMWI	GCNNNNNNNGC	9 cut(s)	114, 123, 216, 326, 437, 486, 759, 768, 980
BstNI	CCWGG	1 cut(s)	1146
BstNSI	RCATGY	2 cut(s)	724, 1158
BstSCI	CCNGG	2 cut(s)	571, 1144
BstSFI	CTRYAG	4 cut(s)	115, 603, 1062, 1077
BstSLI	GKGCMC	1 cut(s)	457
BstV1I	GCAGC	8 cut(s)	55, 58, 129, 279, 418, 421, 424, 1063
BstV2I	GAAGAC	2 cut(s)	705, 1035
BstX2I	RGATCY	1 cut(s)	259
BstXI	CCANNNNNNTGG	2 cut(s)	464, 1046
BstYI	RGATCY	1 cut(s)	259
BsuRI	GGCC	3 cut(s)	113, 455, 480
BtgI	CCRYGG	1 cut(s)	463
BtsCI	GGATG	3 cut(s)	195, 343, 508
BtsI	GCAGTG	1 cut(s)	190
BtsIMutI	CAGTG	3 cut(s)	162, 190, 589
Cac8I	GCNNGC	4 cut(s)	115, 173, 212, 722
CaiI	CAGNNNCTG	3 cut(s)	47, 71, 1169
CfoI	GCGC	2 cut(s)	414, 1008
Cfr13I	GGNCC	4 cut(s)	453, 454, 557, 803
Csp6I	GTAC	6 cut(s)	387, 524, 868, 891, 1029, 1149
CviAII	CATG	7 cut(s)	176, 232, 464, 566, 721, 773, 1155
CviQI	GTAC	6 cut(s)	387, 524, 868, 891, 1029, 1149
DdeI	CTNAG	3 cut(s)	520, 843, 944
DpnI	GATC	3 cut(s)	150, 261, 493
DpnII	GATC	3 cut(s)	148, 259, 491
DriI	GACNNNNNGTC	1 cut(s)	1039
Eam1104I	CTCTTC	1 cut(s)	402
Eam1105I	GACNNNNNGTC	1 cut(s)	1039
EarI	CTCTTC	1 cut(s)	402
Ecl136II	GAGCTC	1 cut(s)	762
Eco130I	CCWWGG	2 cut(s)	352, 463
Eco147I	AGGCCT	1 cut(s)	113
Eco24I	GRGCYC	2 cut(s)	457, 764
Eco32I	GATATC	1 cut(s)	63
Eco47I	GGWCC	2 cut(s)	557, 803
Eco53kI	GAGCTC	1 cut(s)	762
Eco57I	CTGAAG	2 cut(s)	138, 972
EcoICRI	GAGCTC	1 cut(s)	762
EcoO109I	RGGNCCY	1 cut(s)	453
EcoRII	CCWGG	1 cut(s)	1144
EcoRV	GATATC	1 cut(s)	63
EcoT14I	CCWWGG	2 cut(s)	352, 463
EcoT22I	ATGCAT	1 cut(s)	726
EcoT38I	GRGCYC	2 cut(s)	457, 764
ErhI	CCWWGG	2 cut(s)	352, 463
Esp3I	CGTCTC	1 cut(s)	633
FaeI	CATG	7 cut(s)	179, 235, 467, 569, 724, 776, 1158
FaqI	GGGAC	1 cut(s)	213
FatI	CATG	7 cut(s)	175, 231, 463, 565, 720, 772, 1154
FauNDI	CATATG	2 cut(s)	57, 122
Fnu4HI	GCNGC	8 cut(s)	69, 72, 118, 268, 432, 435, 438, 1077
FokI	GGATG	3 cut(s)	182, 330, 495
FriOI	GRGCYC	2 cut(s)	457, 764
Fsp4HI	GCNGC	8 cut(s)	69, 72, 118, 268, 432, 435, 438, 1077
FspBI	CTAG	3 cut(s)	540, 750, 768
GlaI	GCGC	2 cut(s)	413, 1007
GluI	GCNGC	8 cut(s)	69, 72, 118, 268, 432, 435, 438, 1077
GsuI	CTGGAG	2 cut(s)	302, 1068
HaeII	RGCGCY	1 cut(s)	1009
HaeIII	GGCC	3 cut(s)	113, 455, 480
HapII	CCGG	1 cut(s)	573
HhaI	GCGC	2 cut(s)	414, 1008
Hin1II	CATG	7 cut(s)	179, 235, 467, 569, 724, 776, 1158
Hin6I	GCGC	2 cut(s)	412, 1006
HinP1I	GCGC	2 cut(s)	412, 1006
HinfI	GANTC	6 cut(s)	787, 830, 875, 1054, 1070, 1108
HpaII	CCGG	1 cut(s)	573
HphI	GGTGA	2 cut(s)	158, 176
Hpy166II	GTNNAC	2 cut(s)	587, 893
Hpy188I	TCNGA	8 cut(s)	634, 681, 811, 829, 837, 940, 952, 1059
Hpy188III	TCNNGA	5 cut(s)	26, 651, 791, 993, 1047
Hpy8I	GTNNAC	2 cut(s)	587, 893
HpyAV	CCTTC	9 cut(s)	77, 162, 250, 444, 465, 470, 537, 681, 898
HpyCH4III	ACNGT	4 cut(s)	584, 604, 988, 1100
HpyF10VI	GCNNNNNNNGC	9 cut(s)	114, 123, 216, 326, 437, 486, 759, 768, 980
HpyF3I	CTNAG	3 cut(s)	520, 843, 944
Hsp92II	CATG	7 cut(s)	179, 235, 467, 569, 724, 776, 1158
HspAI	GCGC	2 cut(s)	412, 1006
Kzo9I	GATC	3 cut(s)	148, 259, 491
LguI	GCTCTTC	1 cut(s)	402
LmnI	GCTCC	2 cut(s)	486, 1003
Lsp1109I	GCAGC	8 cut(s)	55, 58, 129, 279, 418, 421, 424, 1063
LweI	GCATC	1 cut(s)	204
MaeI	CTAG	3 cut(s)	540, 750, 768
MaeIII	GTNAC	2 cut(s)	160, 1130
MalI	GATC	3 cut(s)	150, 261, 493
MboI	GATC	3 cut(s)	148, 259, 491
MboII	GAAGA	5 cut(s)	315, 419, 687, 705, 1035
MflI	RGATCY	1 cut(s)	259
MhlI	GDGCHC	3 cut(s)	457, 662, 764
MluCI	AATT	8 cut(s)	275, 365, 417, 690, 742, 754, 850, 978
MlyI	GAGTC	1 cut(s)	839
MmeI	TCCRAC	1 cut(s)	918
Mph1103I	ATGCAT	1 cut(s)	726
MroXI	GAANNNNTTC	1 cut(s)	694
MseI	TTAA	2 cut(s)	849, 969
MslI	CAYNNNNRTG	2 cut(s)	462, 932
MspA1I	CMGCKG	2 cut(s)	68, 593
MspI	CCGG	1 cut(s)	573
MspR9I	CCNGG	2 cut(s)	573, 1146
MvaI	CCWGG	1 cut(s)	1146
MwoI	GCNNNNNNNGC	9 cut(s)	114, 123, 216, 326, 437, 486, 759, 768, 980
NciI	CCSGG	1 cut(s)	573
NcoI	CCATGG	1 cut(s)	463
NdeI	CATATG	2 cut(s)	57, 122
NdeII	GATC	3 cut(s)	148, 259, 491
NlaIII	CATG	7 cut(s)	179, 235, 467, 569, 724, 776, 1158
NlaIV	GGNNCC	4 cut(s)	209, 261, 455, 488
NmuCI	GTSAC	1 cut(s)	1130
NsiI	ATGCAT	1 cut(s)	726
NspI	RCATGY	2 cut(s)	724, 1158
PaeI	GCATGC	1 cut(s)	724
PceI	AGGCCT	1 cut(s)	113
PciI	ACATGT	1 cut(s)	1154
PciSI	GCTCTTC	1 cut(s)	402
PdmI	GAANNNNTTC	1 cut(s)	694
PfeI	GAWTC	5 cut(s)	787, 875, 1054, 1070, 1108
PflMI	CCANNNNNTGG	1 cut(s)	237
PkrI	GCNGC	8 cut(s)	70, 73, 119, 269, 433, 436, 439, 1078
PleI	GAGTC	1 cut(s)	838
PpsI	GAGTC	1 cut(s)	838
PscI	ACATGT	1 cut(s)	1154
Psp124BI	GAGCTC	1 cut(s)	764
Psp6I	CCWGG	1 cut(s)	1144
PspGI	CCWGG	1 cut(s)	1144
PspN4I	GGNNCC	4 cut(s)	209, 261, 455, 488
PspOMI	GGGCCC	1 cut(s)	453
PspPI	GGNCC	4 cut(s)	453, 454, 557, 803
PstI	CTGCAG	3 cut(s)	119, 1066, 1081
PstNI	CAGNNNCTG	3 cut(s)	47, 71, 1169
PsuI	RGATCY	1 cut(s)	259
PvuII	CAGCTG	2 cut(s)	68, 593
RsaI	GTAC	6 cut(s)	388, 525, 869, 892, 1030, 1150
RsaNI	GTAC	6 cut(s)	387, 524, 868, 891, 1029, 1149
RseI	CAYNNNNRTG	2 cut(s)	462, 932
SacI	GAGCTC	1 cut(s)	764
SapI	GCTCTTC	1 cut(s)	402
SaqAI	TTAA	2 cut(s)	849, 969
SatI	GCNGC	8 cut(s)	69, 72, 118, 268, 432, 435, 438, 1077
Sau3AI	GATC	3 cut(s)	148, 259, 491
Sau96I	GGNCC	4 cut(s)	453, 454, 557, 803
SchI	GAGTC	1 cut(s)	839
ScrFI	CCNGG	2 cut(s)	573, 1146
SduI	GDGCHC	3 cut(s)	457, 662, 764
SfaNI	GCATC	1 cut(s)	204
SfcI	CTRYAG	4 cut(s)	115, 603, 1062, 1077
SinI	GGWCC	2 cut(s)	557, 803
SmiMI	CAYNNNNRTG	2 cut(s)	462, 932
SmlI	CTYRAG	3 cut(s)	128, 200, 1010
SmoI	CTYRAG	3 cut(s)	128, 200, 1010
SphI	GCATGC	1 cut(s)	724
Sse9I	AATT	8 cut(s)	275, 365, 417, 690, 742, 754, 850, 978
SseBI	AGGCCT	1 cut(s)	113
SspMI	CTAG	3 cut(s)	540, 750, 768
SstI	GAGCTC	1 cut(s)	764
StuI	AGGCCT	1 cut(s)	113
StyD4I	CCNGG	2 cut(s)	571, 1144
StyI	CCWWGG	2 cut(s)	352, 463
TaaI	ACNGT	4 cut(s)	584, 604, 988, 1100
TaqI	TCGA	2 cut(s)	884, 994
TasI	AATT	8 cut(s)	275, 365, 417, 690, 742, 754, 850, 978
TatI	WGTACW	1 cut(s)	890
TfiI	GAWTC	5 cut(s)	787, 875, 1054, 1070, 1108
Tru1I	TTAA	2 cut(s)	849, 969
Tru9I	TTAA	2 cut(s)	849, 969
TscAI	CASTG	3 cut(s)	162, 197, 589
TseFI	GTSAC	1 cut(s)	1130
TseI	GCWGC	8 cut(s)	68, 71, 117, 267, 431, 434, 437, 1076
Tsp45I	GTSAC	1 cut(s)	1130
TspDTI	ATGAA	2 cut(s)	329, 554
TspGWI	ACGGA	2 cut(s)	947, 1046
TspRI	CASTG	3 cut(s)	162, 197, 589
Van91I	CCANNNNNTGG	1 cut(s)	237
VpaK11BI	GGWCC	2 cut(s)	557, 803
XapI	RAATTY	2 cut(s)	690, 742
XceI	RCATGY	2 cut(s)	724, 1158
XmnI	GAANNNNTTC	1 cut(s)	694
XspI	CTAG	3 cut(s)	540, 750, 768
Zsp2I	ATGCAT	1 cut(s)	726

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.