Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004240

Physical Location & Seq

Genomic Coordinates Reverse (-)

5014383 .. 5019919

Gene Structure 5537 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1045000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1164 bp

ATGGAGATTCCAGATAAAATGGAGGCAGAGGCTGAAAGTCATATGATATCAGCTGCTGCATTTGTGGAAGGGGGAATACAAGAGGCTTATGGCGAGGCCTGCAGCATATGCCTTGAGGAGTTTTGTGATGGTGATCCTTCAGTGGTTACTTGTTGCAAGCATGAGTTTCACCTCCACTGCATCCTTGAGTGGTGCCAACGAAGCTCTCAATGTCCCATGTGTTGGCAACCCATTAGCTTGAAGGATCCTTGCAGCCAAGAATTACTGGAGGGAGTAGAAAGGGAGAGAAGAATTAGAGAGACCGCAAGAAATGCACCCATATTTCATCATCCCACCCTTGGCGATTTTCAATTTCAGCATTTACCTGTGGGTACTACCAATGCTGAACTTGAAGAGCGCATAATTCAGCACTTGGCTGCTGCTGCTTCTATGGGAAGGGCCCACCACCATGGTAGAAGGGAAGGCCATAGGAGCCGATTATCTGCTCGTGGTCATCCACACATCTCAGTACCTTCTACTCATTCTAGGTCATCTCCTCTTGGTCCTGTTCATGCCCCTGGAGGAGACACTGAACCAGCTGAAATAACTGTAGCAAGTCCATCTACCCCACTTACATCAGATGGAGACGAAACATCACGAATGAGCGCACAATATCCTTCTGTTCATACGGATGGAAATTCTTCCTCGCCATCTGGGTCTTCACGCATGCATAGAAATCGTCAAGGAAATTCCTCTAGCAATTTCTCTACTAGCCATGCCTCCTCTTTGAATCAGGACAGAGCAGGACCATCAGAACTTCACTCTCTTTCAGAGTCTCTGAAATCTAAGTTTAATTCTATGTCAATGAGGTACAAGGAATCGTTTTCGAGGAATACACGAGGATTGAAGGAGAGGCTGTTCTCTCGTAGCACTTCTATGTCGGAACTTAGTTCTGAAGTCCGTAGAGAGGTTAATGCTGGAATTGCCACCGTATCTCGAATGATGGAGCGCCTTGAGACAAGAGATAATAGTCCAGACAACCAGTCTTCTGGAGTGAATCAGACTGCGGATGGTTCTGCTGCAGAGCAGGGCAACCAGAACCGTGCAGAGATTCATGGAGAAAACCCTTTGAGTGACAACAATAGGCCTGGTACTACATGTGCCGCGGGTTCTGCTTCACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

388

Amino Acids

42.24

Weight (kDa)

6.05

Isoelectric Point (pI)

54.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	29 - 74	1.4e-07	RING-H2 zinc finger domain
zf-ANAPC11	PF12861	30 - 81	1.7e-06	Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2	PF13639	33 - 74	7.2e-09	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004240)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g20900 FvH4_6g20900 FvH4_6g20900 FvH4_6g20900 FvH4_6g20900 FvH4_6g20900 FvH4_6g20900
malus_domestica	MD12G1045000.v1.1 MD14G1043800.v1.1
prunus_persica	Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1 Prupe.7G090500_v2.0.a1
pyrus_communis	pycom12g03880 pycom14g03720
rosa_chinensis	RchiOBHm_Chr3g0477251
rosa_laevigata	RLG00000023734
rosa_multiflora	Rmu_co8521747.1_g000001 Rmu_sc0000348.1_g000004
rosa_roxburghii	Rroxscaffold_6G00404290 Rroxscaffold_6G00404770
rosa_rugosa	Rorug03G0159000.1 Rorug03G0159100 Rorug03G0159200 Rorug03G0159300
rosa_samantha	Rh3AG209600 Rh3BG243000 Rh3CG237100 Rh3DG236200
rosa_wichuraiana	Rw0G000250 Rw0G021740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	192
AccB7I	CCANNNNNTGG	1 cut(s)	222
AccII	CGCG	1 cut(s)	1145
AciI	CCGC	4 cut(s)	303, 1046, 1143, 1145
AclWI	GGATC	3 cut(s)	128, 239, 252
AcsI	RAATTY	2 cut(s)	676, 727
AcuI	CTGAAG	2 cut(s)	123, 954
AfaI	GTAC	4 cut(s)	373, 510, 851, 1132
AfiI	CCNNNNNNNGG	3 cut(s)	222, 338, 946
AflIII	ACRYGT	1 cut(s)	1136
AgsI	TTSAA	5 cut(s)	241, 350, 392, 769, 886
AhdI	GACNNNNNGTC	1 cut(s)	1021
AjnI	CCWGG	2 cut(s)	556, 1126
AloI	GAACNNNNNNTCC	2 cut(s)	881, 913
AluBI	AGCT	4 cut(s)	53, 204, 237, 578
AluI	AGCT	4 cut(s)	53, 204, 237, 578
Alw26I	GTCTC	5 cut(s)	293, 558, 618, 819, 989
AlwI	GGATC	3 cut(s)	128, 239, 252
AlwNI	CAGNNNCTG	2 cut(s)	32, 56
AoxI	GGCC	4 cut(s)	96, 438, 463, 1124
ApaI	GGGCCC	1 cut(s)	442
ApeKI	GCWGC	8 cut(s)	53, 56, 102, 252, 416, 419, 422, 1058
ApoI	RAATTY	2 cut(s)	676, 727
Asp700I	GAANNNNTTC	1 cut(s)	679
AspLEI	GCGC	3 cut(s)	399, 647, 990
AspS9I	GGNCC	4 cut(s)	438, 439, 542, 785
AsuHPI	GGTGA	2 cut(s)	143, 161
AvaII	GGWCC	2 cut(s)	542, 785
BaeGI	GKGCMC	1 cut(s)	442
BamHI	GGATCC	1 cut(s)	244
BanI	GGYRCC	1 cut(s)	192
BanII	GRGCYC	1 cut(s)	442
BarI	GAAGNNNNNNTAC	2 cut(s)	60, 92
BauI	CACGAG	2 cut(s)	486, 876
BbsI	GAAGAC	2 cut(s)	690, 1017
BbvI	GCAGC	8 cut(s)	40, 43, 114, 264, 403, 406, 409, 1045
BccI	CCATC	8 cut(s)	122, 607, 614, 665, 697, 796, 976, 1043
BcgI	CGANNNNNNTGC	2 cut(s)	698, 732
BciT130I	CCWGG	2 cut(s)	558, 1128
BcoDI	GTCTC	5 cut(s)	293, 558, 618, 819, 989
BfaI	CTAG	3 cut(s)	525, 735, 750
BfmI	CTRYAG	3 cut(s)	100, 588, 1059
BfoI	RGCGCY	1 cut(s)	991
BisI	GCNGC	9 cut(s)	54, 57, 103, 253, 417, 420, 423, 1059, 1143
BlsI	GCNGC	9 cut(s)	55, 58, 104, 254, 418, 421, 424, 1060, 1144
Bme1390I	CCNGG	2 cut(s)	558, 1128
Bme18I	GGWCC	2 cut(s)	542, 785
BmeRI	GACNNNNNGTC	1 cut(s)	1021
BmgT120I	GGNCC	4 cut(s)	438, 439, 542, 785
BmiI	GGNNCC	4 cut(s)	194, 246, 440, 473
BmrFI	CCNGG	2 cut(s)	558, 1128
BmsI	GCATC	1 cut(s)	189
BpiI	GAAGAC	2 cut(s)	690, 1017
BpmI	CTGGAG	3 cut(s)	287, 579, 1050
BpuEI	CTTGAG	3 cut(s)	134, 206, 1013
BsaBI	GATNNNNATC	1 cut(s)	132
BsaI	GGTCTC	1 cut(s)	293
BsaJI	CCNNGG	4 cut(s)	337, 448, 556, 1143
BsaXI	ACNNNNNCTCC	2 cut(s)	881, 911
Bsc4I	CCNNNNNNNGG	3 cut(s)	222, 338, 946
Bse1I	ACTGG	2 cut(s)	270, 1021
Bse8I	GATNNNNATC	1 cut(s)	132
BseBI	CCWGG	2 cut(s)	558, 1128
BseDI	CCNNGG	4 cut(s)	337, 448, 556, 1143
BseGI	GGATG	5 cut(s)	180, 328, 493, 676, 1054
BseJI	GATNNNNATC	1 cut(s)	132
BseLI	CCNNNNNNNGG	3 cut(s)	222, 338, 946
BseMII	CTCAG	1 cut(s)	519
BseNI	ACTGG	2 cut(s)	270, 1021
BseRI	GAGGAG	4 cut(s)	131, 525, 576, 751
BseSI	GKGCMC	1 cut(s)	442
BseXI	GCAGC	8 cut(s)	40, 43, 114, 264, 403, 406, 409, 1045
BsgI	GTGCAG	1 cut(s)	1104
Bsh1236I	CGCG	1 cut(s)	1145
BshFI	GGCC	4 cut(s)	98, 440, 465, 1126
BshNI	GGYRCC	1 cut(s)	192
BslFI	GGGAC	1 cut(s)	198
BslI	CCNNNNNNNGG	3 cut(s)	222, 338, 946
BsmAI	GTCTC	5 cut(s)	293, 558, 618, 819, 989
BsmBI	CGTCTC	1 cut(s)	618
BsmFI	GGGAC	1 cut(s)	198
BsnI	GGCC	4 cut(s)	98, 440, 465, 1126
Bso31I	GGTCTC	1 cut(s)	293
Bsp120I	GGGCCC	1 cut(s)	438
Bsp1286I	GDGCHC	1 cut(s)	442
Bsp143I	GATC	2 cut(s)	133, 244
Bsp19I	CCATGG	1 cut(s)	448
BspACI	CCGC	4 cut(s)	303, 1046, 1143, 1145
BspANI	GGCC	4 cut(s)	98, 440, 465, 1126
BspCNI	CTCAG	1 cut(s)	518
BspFNI	CGCG	1 cut(s)	1145
BspLI	GGNNCC	4 cut(s)	194, 246, 440, 473
BspMAI	CTGCAG	2 cut(s)	104, 1063
BspPI	GGATC	3 cut(s)	128, 239, 252
BspQI	GCTCTTC	1 cut(s)	387
BspT107I	GGYRCC	1 cut(s)	192
BspTNI	GGTCTC	1 cut(s)	293
BsrI	ACTGG	2 cut(s)	270, 1021
BssECI	CCNNGG	4 cut(s)	337, 448, 556, 1143
BssMI	GATC	2 cut(s)	133, 244
BssSI	CACGAG	2 cut(s)	486, 876
BssT1I	CCWWGG	2 cut(s)	337, 448
Bst2BI	CACGAG	2 cut(s)	486, 876
Bst2UI	CCWGG	2 cut(s)	558, 1128
Bst4CI	ACNGT	3 cut(s)	589, 970, 1082
Bst6I	CTCTTC	1 cut(s)	387
BstAPI	GCANNNNNTGC	2 cut(s)	108, 311
BstC8I	GCNNGC	3 cut(s)	100, 158, 707
BstDEI	CTNAG	3 cut(s)	505, 825, 926
BstDSI	CCRYGG	2 cut(s)	448, 1143
BstF5I	GGATG	5 cut(s)	180, 328, 493, 676, 1054
BstFNI	CGCG	1 cut(s)	1145
BstH2I	RGCGCY	1 cut(s)	991
BstHHI	GCGC	3 cut(s)	399, 647, 990
BstKTI	GATC	2 cut(s)	136, 247
BstMAI	GTCTC	5 cut(s)	293, 558, 618, 819, 989
BstMBI	GATC	2 cut(s)	133, 244
BstMWI	GCNNNNNNNGC	8 cut(s)	99, 108, 201, 311, 422, 471, 962, 1151
BstNI	CCWGG	2 cut(s)	558, 1128
BstNSI	RCATGY	2 cut(s)	709, 1140
BstSCI	CCNGG	2 cut(s)	556, 1126
BstSFI	CTRYAG	3 cut(s)	100, 588, 1059
BstSLI	GKGCMC	1 cut(s)	442
BstUI	CGCG	1 cut(s)	1145
BstV1I	GCAGC	8 cut(s)	40, 43, 114, 264, 403, 406, 409, 1045
BstV2I	GAAGAC	2 cut(s)	690, 1017
BstX2I	RGATCY	1 cut(s)	244
BstXI	CCANNNNNNTGG	2 cut(s)	449, 1028
BstYI	RGATCY	1 cut(s)	244
BsuRI	GGCC	4 cut(s)	98, 440, 465, 1126
BtgI	CCRYGG	2 cut(s)	448, 1143
BtsCI	GGATG	5 cut(s)	180, 328, 493, 676, 1054
BtsI	GCAGTG	1 cut(s)	175
BtsIMutI	CAGTG	3 cut(s)	147, 175, 567
Cac8I	GCNNGC	3 cut(s)	100, 158, 707
CaiI	CAGNNNCTG	2 cut(s)	32, 56
CfoI	GCGC	3 cut(s)	399, 647, 990
Cfr13I	GGNCC	4 cut(s)	438, 439, 542, 785
Cfr42I	CCGCGG	1 cut(s)	1146
Csp6I	GTAC	4 cut(s)	372, 509, 850, 1131
CviAII	CATG	8 cut(s)	161, 217, 449, 551, 706, 755, 1094, 1137
CviQI	GTAC	4 cut(s)	372, 509, 850, 1131
DdeI	CTNAG	3 cut(s)	505, 825, 926
DpnI	GATC	2 cut(s)	135, 246
DpnII	GATC	2 cut(s)	133, 244
DriI	GACNNNNNGTC	1 cut(s)	1021
Eam1104I	CTCTTC	1 cut(s)	387
Eam1105I	GACNNNNNGTC	1 cut(s)	1021
EarI	CTCTTC	1 cut(s)	387
Eco130I	CCWWGG	2 cut(s)	337, 448
Eco147I	AGGCCT	2 cut(s)	98, 1126
Eco24I	GRGCYC	1 cut(s)	442
Eco31I	GGTCTC	1 cut(s)	293
Eco32I	GATATC	1 cut(s)	48
Eco47I	GGWCC	2 cut(s)	542, 785
Eco57I	CTGAAG	2 cut(s)	123, 954
EcoO109I	RGGNCCY	1 cut(s)	438
EcoRII	CCWGG	2 cut(s)	556, 1126
EcoRV	GATATC	1 cut(s)	48
EcoT14I	CCWWGG	2 cut(s)	337, 448
EcoT22I	ATGCAT	1 cut(s)	711
EcoT38I	GRGCYC	1 cut(s)	442
ErhI	CCWWGG	2 cut(s)	337, 448
Esp3I	CGTCTC	1 cut(s)	618
FaeI	CATG	8 cut(s)	164, 220, 452, 554, 709, 758, 1097, 1140
FaqI	GGGAC	1 cut(s)	198
FatI	CATG	8 cut(s)	160, 216, 448, 550, 705, 754, 1093, 1136
FauI	CCCGC	1 cut(s)	1138
FauNDI	CATATG	2 cut(s)	42, 107
Fnu4HI	GCNGC	9 cut(s)	54, 57, 103, 253, 417, 420, 423, 1059, 1143
FokI	GGATG	5 cut(s)	167, 315, 480, 683, 1061
FriOI	GRGCYC	1 cut(s)	442
Fsp4HI	GCNGC	9 cut(s)	54, 57, 103, 253, 417, 420, 423, 1059, 1143
FspBI	CTAG	3 cut(s)	525, 735, 750
GlaI	GCGC	3 cut(s)	398, 646, 989
GluI	GCNGC	9 cut(s)	54, 57, 103, 253, 417, 420, 423, 1059, 1143
GsuI	CTGGAG	3 cut(s)	287, 579, 1050
HaeII	RGCGCY	1 cut(s)	991
HaeIII	GGCC	4 cut(s)	98, 440, 465, 1126
HhaI	GCGC	3 cut(s)	399, 647, 990
Hin1II	CATG	8 cut(s)	164, 220, 452, 554, 709, 758, 1097, 1140
Hin6I	GCGC	3 cut(s)	397, 645, 988
HinP1I	GCGC	3 cut(s)	397, 645, 988
HinfI	GANTC	6 cut(s)	7, 769, 812, 857, 1036, 1090
HphI	GGTGA	2 cut(s)	143, 161
Hpy188I	TCNGA	7 cut(s)	619, 793, 811, 819, 922, 934, 1041
Hpy188III	TCNNGA	6 cut(s)	11, 636, 773, 975, 1013, 1029
HpyAV	CCTTC	9 cut(s)	62, 147, 235, 429, 450, 455, 522, 666, 880
HpyCH4III	ACNGT	3 cut(s)	589, 970, 1082
HpyCH4V	TGCA	9 cut(s)	59, 102, 156, 180, 252, 314, 709, 1061, 1085
HpyF10VI	GCNNNNNNNGC	8 cut(s)	99, 108, 201, 311, 422, 471, 962, 1151
HpyF3I	CTNAG	3 cut(s)	505, 825, 926
Hsp92II	CATG	8 cut(s)	164, 220, 452, 554, 709, 758, 1097, 1140
HspAI	GCGC	3 cut(s)	397, 645, 988
KspI	CCGCGG	1 cut(s)	1146
Kzo9I	GATC	2 cut(s)	133, 244
LguI	GCTCTTC	1 cut(s)	387
LmnI	GCTCC	2 cut(s)	471, 985
Lsp1109I	GCAGC	8 cut(s)	40, 43, 114, 264, 403, 406, 409, 1045
LweI	GCATC	1 cut(s)	189
MaeI	CTAG	3 cut(s)	525, 735, 750
MaeIII	GTNAC	2 cut(s)	145, 1112
MalI	GATC	2 cut(s)	135, 246
MboI	GATC	2 cut(s)	133, 244
MboII	GAAGA	5 cut(s)	300, 404, 672, 690, 1017
MflI	RGATCY	1 cut(s)	244
MhlI	GDGCHC	1 cut(s)	442
MluCI	AATT	9 cut(s)	260, 291, 350, 402, 676, 727, 739, 832, 960
MlyI	GAGTC	1 cut(s)	821
MmeI	TCCRAC	1 cut(s)	900
Mph1103I	ATGCAT	1 cut(s)	711
MroXI	GAANNNNTTC	1 cut(s)	679
MseI	TTAA	2 cut(s)	831, 951
MslI	CAYNNNNRTG	3 cut(s)	447, 669, 914
MspA1I	CMGCKG	3 cut(s)	53, 578, 1145
MspR9I	CCNGG	2 cut(s)	558, 1128
MvaI	CCWGG	2 cut(s)	558, 1128
MvnI	CGCG	1 cut(s)	1145
MwoI	GCNNNNNNNGC	8 cut(s)	99, 108, 201, 311, 422, 471, 962, 1151
NcoI	CCATGG	1 cut(s)	448
NdeI	CATATG	2 cut(s)	42, 107
NdeII	GATC	2 cut(s)	133, 244
NlaIII	CATG	8 cut(s)	164, 220, 452, 554, 709, 758, 1097, 1140
NlaIV	GGNNCC	4 cut(s)	194, 246, 440, 473
NmuCI	GTSAC	1 cut(s)	1112
NsiI	ATGCAT	1 cut(s)	711
NspI	RCATGY	2 cut(s)	709, 1140
PaeI	GCATGC	1 cut(s)	709
PceI	AGGCCT	2 cut(s)	98, 1126
PciI	ACATGT	1 cut(s)	1136
PciSI	GCTCTTC	1 cut(s)	387
PdmI	GAANNNNTTC	1 cut(s)	679
PfeI	GAWTC	5 cut(s)	7, 769, 857, 1036, 1090
PflMI	CCANNNNNTGG	1 cut(s)	222
PkrI	GCNGC	9 cut(s)	55, 58, 104, 254, 418, 421, 424, 1060, 1144
PleI	GAGTC	1 cut(s)	820
PpsI	GAGTC	1 cut(s)	820
PscI	ACATGT	1 cut(s)	1136
Psp6I	CCWGG	2 cut(s)	556, 1126
PspGI	CCWGG	2 cut(s)	556, 1126
PspN4I	GGNNCC	4 cut(s)	194, 246, 440, 473
PspOMI	GGGCCC	1 cut(s)	438
PspPI	GGNCC	4 cut(s)	438, 439, 542, 785
PstI	CTGCAG	2 cut(s)	104, 1063
PstNI	CAGNNNCTG	2 cut(s)	32, 56
PsuI	RGATCY	1 cut(s)	244
PvuII	CAGCTG	2 cut(s)	53, 578
RsaI	GTAC	4 cut(s)	373, 510, 851, 1132
RsaNI	GTAC	4 cut(s)	372, 509, 850, 1131
RseI	CAYNNNNRTG	3 cut(s)	447, 669, 914
SacII	CCGCGG	1 cut(s)	1146
SapI	GCTCTTC	1 cut(s)	387
SaqAI	TTAA	2 cut(s)	831, 951
SatI	GCNGC	9 cut(s)	54, 57, 103, 253, 417, 420, 423, 1059, 1143
Sau3AI	GATC	2 cut(s)	133, 244
Sau96I	GGNCC	4 cut(s)	438, 439, 542, 785
SchI	GAGTC	1 cut(s)	821
ScrFI	CCNGG	2 cut(s)	558, 1128
SduI	GDGCHC	1 cut(s)	442
SfaNI	GCATC	1 cut(s)	189
SfcI	CTRYAG	3 cut(s)	100, 588, 1059
Sfr303I	CCGCGG	1 cut(s)	1146
SgrBI	CCGCGG	1 cut(s)	1146
SinI	GGWCC	2 cut(s)	542, 785
SmiMI	CAYNNNNRTG	3 cut(s)	447, 669, 914
SmlI	CTYRAG	3 cut(s)	113, 185, 992
SmoI	CTYRAG	3 cut(s)	113, 185, 992
SphI	GCATGC	1 cut(s)	709
Sse9I	AATT	9 cut(s)	260, 291, 350, 402, 676, 727, 739, 832, 960
SseBI	AGGCCT	2 cut(s)	98, 1126
SsiI	CCGC	4 cut(s)	303, 1046, 1143, 1145
SspMI	CTAG	3 cut(s)	525, 735, 750
StuI	AGGCCT	2 cut(s)	98, 1126
StyD4I	CCNGG	2 cut(s)	556, 1126
StyI	CCWWGG	2 cut(s)	337, 448
TaaI	ACNGT	3 cut(s)	589, 970, 1082
TaqI	TCGA	2 cut(s)	866, 976
TasI	AATT	9 cut(s)	260, 291, 350, 402, 676, 727, 739, 832, 960
TauI	GCSGC	1 cut(s)	1145
TfiI	GAWTC	5 cut(s)	7, 769, 857, 1036, 1090
Tru1I	TTAA	2 cut(s)	831, 951
Tru9I	TTAA	2 cut(s)	831, 951
TscAI	CASTG	3 cut(s)	147, 182, 574
TseFI	GTSAC	1 cut(s)	1112
TseI	GCWGC	8 cut(s)	53, 56, 102, 252, 416, 419, 422, 1058
Tsp45I	GTSAC	1 cut(s)	1112
TspDTI	ATGAA	4 cut(s)	314, 539, 653, 1082
TspGWI	ACGGA	2 cut(s)	683, 929
TspRI	CASTG	3 cut(s)	147, 182, 574
Van91I	CCANNNNNTGG	1 cut(s)	222
VpaK11BI	GGWCC	2 cut(s)	542, 785
XapI	RAATTY	2 cut(s)	676, 727
XceI	RCATGY	2 cut(s)	709, 1140
XmnI	GAANNNNTTC	1 cut(s)	679
XspI	CTAG	3 cut(s)	525, 735, 750
Zsp2I	ATGCAT	1 cut(s)	711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.