Rroxscaffold_6G00404770

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004240
Physical Location & Seq
Forward (+)
27287535 .. 27292102
4568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404770.1

Sequence Viewer

Length: 1212 bp
ATGAATGGCTCTTCTTCTCTGAATTTCTTGACTACCAATTCTAATCCCTTAATGGAGGTTCCGGATATGACGGAGGCAAAGAAGGCTGAAACTCAATTGATATCAGCTGCTGCTTTTGTGGAAGGGGGAATACAAGATGCATGTGGTGAAGCCTGTAGCATATGCCTTGAGGATTTTTGTGAAAGTGATCCTTCAGTGCTGACTACTTGCAAGCATGAGTTTCACTTCCACTGCATCCTGGAATGGTGCCAGAGAAGTTCCCAATGTCCGATGTGTTGGCAAGCCCTTAGCCTGAAGGATCCTTCCAGTCAAGAATTACTGGAGGCAGTAGAACGGGAGAGGAGCATTAGGGCCACATCAAGAAATGCAGCTATATTCCATCATCCCACCCTTGGCGAATTTGAAGTGCAGCATTTACCTGTGGGTACTAATGATGCTGACCTTGAAGAGCGTATCATCCAGCACTTGGCAGCTGCTGCTTCAATGGGAAGGGCTCACCACATTGGTAGAAGGGAAGGCCAGAGGAACCGGTCATCTGTTCGTGGTCATCCACACTTCTCAGTTATTTCCACTCATCATAGTGCAGCTTCACTTGGTCATGTTTCTGCCTCGGGTGGGGAGAGTGAACCAGCTATCATAACTGCAGCAAGTCCATCTATCCCACTTACATCAGGTGGAAATGAATCATCACGATGGAGTTCACAAGTTTCTTCTGTTCAAACTGATGGGTTTTCTTCCTCCCCACCTGGGCCTACACTTATGCAAAGAAATCGTGTTCCCTCTAGTCGCTGGAGCTCTACTAGTCAGTCCTCACCAATGAATCAGGAAAGTGCAGGACCATCAGATCTTCAGTCTATTTCAGAGTCTCTGAAATCAAAATTCAATGCCATGTCAATGAAATATAAGGAGTCATTTTCAAAGAGTACAAGAGGCTGGAAGGAGAGGCTGTTCTCTCGTAGCACTTCTATGTCAGAACTTGGTTCCGAAGTCCGGAGAGAAGTCAATGCTGGAATTGCTAGTGTATCTCGAATTATAGAGCGCCTTGAGACCAGAGATAATAGTAGAGTTAACCAGGATCCTGTAGCAAATCCAACAGCAGATGATTCTTTGACTGACCAGGGAAACCAGAACAGCGCTGAGAGTCATGGAGGAAGTTTGACCAATAACAATAGGCCTGCAACAACTTGTGCTGCAGGTCCTGCTTCCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

403

Amino Acids

43.49

Weight (kDa)

5.93

Isoelectric Point (pI)

56.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 48 - 92 1.3e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 51 - 92 7.6e-10 Ring finger domain
zf-RING_11 PF17123 52 - 79 9.3e-07 RING-like zinc finger
zf-C3HC4 PF00097 52 - 92 6.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 63 - 99 1.5e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1184
AccB1I GGYRCC 1 cut(s) 246
AccB7I CCANNNNNTGG 1 cut(s) 466
AccIII TCCGGA 2 cut(s) 61, 990
AclWI GGATC 5 cut(s) 182, 293, 306, 1070, 1083
AcsI RAATTY 3 cut(s) 22, 398, 878
AcuI CTGAAG 3 cut(s) 177, 314, 833
AfaI GTAC 2 cut(s) 427, 925
AfeI AGCGCT 1 cut(s) 1135
AfiI CCNNNNNNNGG 5 cut(s) 392, 466, 615, 747, 990
AgeI ACCGGT 1 cut(s) 528
AgsI TTSAA 6 cut(s) 404, 446, 483, 719, 883, 918
AhlI ACTAGT 1 cut(s) 800
AjnI CCWGG 4 cut(s) 237, 745, 1071, 1116
AloI GAACNNNNNNTCC 2 cut(s) 932, 964
AluBI AGCT 6 cut(s) 107, 371, 473, 587, 632, 795
AluI AGCT 6 cut(s) 107, 371, 473, 587, 632, 795
Alw21I GWGCWC 1 cut(s) 797
Alw26I GTCTC 2 cut(s) 870, 1040
AlwI GGATC 5 cut(s) 182, 293, 306, 1070, 1083
AlwNI CAGNNNCTG 3 cut(s) 110, 476, 1199
Ama87I CYCGRG 1 cut(s) 610
Aor13HI TCCGGA 2 cut(s) 61, 990
Aor51HI AGCGCT 1 cut(s) 1135
AoxI GGCC 4 cut(s) 351, 517, 749, 1172
ApoI RAATTY 3 cut(s) 22, 398, 878
AsiGI ACCGGT 1 cut(s) 528
AspLEI GCGC 2 cut(s) 1041, 1136
AspS9I GGNCC 4 cut(s) 351, 749, 836, 1196
AsuHPI GGTGA 3 cut(s) 158, 488, 804
AvaI CYCGRG 1 cut(s) 610
AvaII GGWCC 2 cut(s) 836, 1196
BamHI GGATCC 2 cut(s) 298, 1075
BanI GGYRCC 1 cut(s) 246
BanII GRGCYC 2 cut(s) 496, 797
BarI GAAGNNNNNNTAC 2 cut(s) 114, 146
Bbv12I GWGCWC 1 cut(s) 797
BccI CCATC 5 cut(s) 387, 661, 687, 719, 847
BcgI CGANNNNNNTGC 2 cut(s) 752, 786
BciT130I CCWGG 4 cut(s) 239, 747, 1073, 1118
BcoDI GTCTC 2 cut(s) 870, 1040
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 3 cut(s) 783, 801, 1017
BfmI CTRYAG 4 cut(s) 154, 642, 1080, 1191
BfoI RGCGCY 2 cut(s) 1042, 1137
BfuAI ACCTGC 1 cut(s) 1184
BglII AGATCT 1 cut(s) 844
Bme1390I CCNGG 4 cut(s) 239, 747, 1073, 1118
Bme18I GGWCC 2 cut(s) 836, 1196
BmeT110I CYCGRG 1 cut(s) 610
BmgT120I GGNCC 4 cut(s) 351, 749, 836, 1196
BmiI GGNNCC 6 cut(s) 60, 248, 300, 527, 982, 1077
BmrFI CCNGG 4 cut(s) 239, 747, 1073, 1118
BmsI GCATC 3 cut(s) 127, 243, 424
BpmI CTGGAG 2 cut(s) 341, 811
Bpu10I CCTNAGC 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 188, 1064
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 4 cut(s) 391, 609, 746, 1117
BsaWI WCCGGW 3 cut(s) 61, 528, 990
BsaXI ACNNNNNCTCC 2 cut(s) 932, 962
Bsc4I CCNNNNNNNGG 5 cut(s) 392, 466, 615, 747, 990
Bse118I RCCGGY 1 cut(s) 528
Bse1I ACTGG 2 cut(s) 306, 324
BseAI TCCGGA 2 cut(s) 61, 990
BseBI CCWGG 4 cut(s) 239, 747, 1073, 1118
BseDI CCNNGG 4 cut(s) 391, 609, 746, 1117
BseGI GGATG 4 cut(s) 234, 382, 456, 547
BseLI CCNNNNNNNGG 5 cut(s) 392, 466, 615, 747, 990
BseMII CTCAG 2 cut(s) 573, 1128
BseNI ACTGG 2 cut(s) 306, 324
BseRI GAGGAG 1 cut(s) 355
BsgI GTGCAG 3 cut(s) 428, 603, 852
BshFI GGCC 4 cut(s) 353, 519, 751, 1174
BshNI GGYRCC 1 cut(s) 246
BshTI ACCGGT 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 797
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 3 cut(s) 62, 529, 991
BslI CCNNNNNNNGG 5 cut(s) 392, 466, 615, 747, 990
BsmAI GTCTC 2 cut(s) 870, 1040
BsnI GGCC 4 cut(s) 353, 519, 751, 1174
Bso31I GGTCTC 1 cut(s) 1040
BsoBI CYCGRG 1 cut(s) 610
Bsp1286I GDGCHC 2 cut(s) 496, 797
Bsp13I TCCGGA 2 cut(s) 61, 990
Bsp143I GATC 4 cut(s) 187, 298, 844, 1075
BspANI GGCC 4 cut(s) 353, 519, 751, 1174
BspCNI CTCAG 2 cut(s) 572, 1129
BspEI TCCGGA 2 cut(s) 61, 990
BspLI GGNNCC 6 cut(s) 60, 248, 300, 527, 982, 1077
BspMAI CTGCAG 2 cut(s) 646, 1195
BspMI ACCTGC 1 cut(s) 1184
BspPI GGATC 5 cut(s) 182, 293, 306, 1070, 1083
BspQI GCTCTTC 2 cut(s) 16, 441
BspT107I GGYRCC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 1040
BsrFI RCCGGY 1 cut(s) 528
BsrI ACTGG 2 cut(s) 306, 324
BssAI RCCGGY 1 cut(s) 528
BssECI CCNNGG 4 cut(s) 391, 609, 746, 1117
BssMI GATC 4 cut(s) 187, 298, 844, 1075
BssT1I CCWWGG 1 cut(s) 391
Bst2UI CCWGG 4 cut(s) 239, 747, 1073, 1118
Bst6I CTCTTC 2 cut(s) 16, 441
BstAPI GCANNNNNTGC 2 cut(s) 476, 1199
BstC8I GCNNGC 3 cut(s) 212, 282, 1176
BstDEI CTNAG 3 cut(s) 287, 559, 1137
BstF5I GGATG 4 cut(s) 234, 382, 456, 547
BstH2I RGCGCY 2 cut(s) 1042, 1137
BstHHI GCGC 2 cut(s) 1041, 1136
BstKTI GATC 4 cut(s) 190, 301, 847, 1078
BstMAI GTCTC 2 cut(s) 870, 1040
BstMBI GATC 4 cut(s) 187, 298, 844, 1075
BstMWI GCNNNNNNNGC 4 cut(s) 83, 476, 1013, 1199
BstNI CCWGG 4 cut(s) 239, 747, 1073, 1118
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 4 cut(s) 237, 745, 1071, 1116
BstSFI CTRYAG 4 cut(s) 154, 642, 1080, 1191
BstX2I RGATCY 3 cut(s) 298, 844, 1075
BstYI RGATCY 3 cut(s) 298, 844, 1075
BsuRI GGCC 4 cut(s) 353, 519, 751, 1174
BtsCI GGATG 4 cut(s) 234, 382, 456, 547
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 2 cut(s) 201, 229
BveI ACCTGC 1 cut(s) 1184
Cac8I GCNNGC 3 cut(s) 212, 282, 1176
CaiI CAGNNNCTG 3 cut(s) 110, 476, 1199
CfoI GCGC 2 cut(s) 1041, 1136
Cfr10I RCCGGY 1 cut(s) 528
Cfr13I GGNCC 4 cut(s) 351, 749, 836, 1196
Csp6I GTAC 2 cut(s) 426, 924
CspAI ACCGGT 1 cut(s) 528
CviAII CATG 5 cut(s) 141, 215, 599, 889, 1145
CviQI GTAC 2 cut(s) 426, 924
DdeI CTNAG 3 cut(s) 287, 559, 1137
DpnI GATC 4 cut(s) 189, 300, 846, 1077
DpnII GATC 4 cut(s) 187, 298, 844, 1075
Eam1104I CTCTTC 2 cut(s) 16, 441
EarI CTCTTC 2 cut(s) 16, 441
Ecl136II GAGCTC 1 cut(s) 795
Eco130I CCWWGG 1 cut(s) 391
Eco147I AGGCCT 1 cut(s) 1174
Eco24I GRGCYC 2 cut(s) 496, 797
Eco31I GGTCTC 1 cut(s) 1040
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 2 cut(s) 836, 1196
Eco47III AGCGCT 1 cut(s) 1135
Eco53kI GAGCTC 1 cut(s) 795
Eco57I CTGAAG 3 cut(s) 177, 314, 833
Eco88I CYCGRG 1 cut(s) 610
EcoICRI GAGCTC 1 cut(s) 795
EcoO109I RGGNCCY 1 cut(s) 1196
EcoRII CCWGG 4 cut(s) 237, 745, 1071, 1116
EcoRV GATATC 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 391
EcoT22I ATGCAT 1 cut(s) 142
EcoT38I GRGCYC 2 cut(s) 496, 797
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 5 cut(s) 144, 218, 602, 892, 1148
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FatI CATG 5 cut(s) 140, 214, 598, 888, 1144
FauNDI CATATG 1 cut(s) 161
FokI GGATG 4 cut(s) 221, 369, 443, 534
FriOI GRGCYC 2 cut(s) 496, 797
FspBI CTAG 3 cut(s) 783, 801, 1017
GlaI GCGC 2 cut(s) 1040, 1135
GsuI CTGGAG 2 cut(s) 341, 811
HaeII RGCGCY 2 cut(s) 1042, 1137
HaeIII GGCC 4 cut(s) 353, 519, 751, 1174
HapII CCGG 3 cut(s) 62, 529, 991
HhaI GCGC 2 cut(s) 1041, 1136
Hin1II CATG 5 cut(s) 144, 218, 602, 892, 1148
Hin6I GCGC 2 cut(s) 1039, 1134
HinP1I GCGC 2 cut(s) 1039, 1134
HincII GTYRAC 1 cut(s) 1069
HindII GTYRAC 1 cut(s) 1069
HinfI GANTC 6 cut(s) 683, 820, 863, 908, 1103, 1141
HpaI GTTAAC 1 cut(s) 1069
HpaII CCGG 3 cut(s) 62, 529, 991
HphI GGTGA 3 cut(s) 158, 488, 804
Hpy166II GTNNAC 3 cut(s) 626, 701, 1069
Hpy188I TCNGA 7 cut(s) 21, 270, 844, 862, 870, 973, 985
Hpy188III TCNNGA 8 cut(s) 28, 62, 311, 360, 690, 824, 991, 1026
Hpy8I GTNNAC 3 cut(s) 626, 701, 1069
HpyAV CCTTC 9 cut(s) 76, 116, 201, 289, 312, 483, 504, 509, 931
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 476, 1013, 1199
HpyF3I CTNAG 3 cut(s) 287, 559, 1137
Hsp92II CATG 5 cut(s) 144, 218, 602, 892, 1148
HspAI GCGC 2 cut(s) 1039, 1134
Kpn2I TCCGGA 2 cut(s) 61, 990
KspAI GTTAAC 1 cut(s) 1069
Kzo9I GATC 4 cut(s) 187, 298, 844, 1075
LguI GCTCTTC 2 cut(s) 16, 441
LmnI GCTCC 2 cut(s) 342, 792
LweI GCATC 3 cut(s) 127, 243, 424
MaeI CTAG 3 cut(s) 783, 801, 1017
MalI GATC 4 cut(s) 189, 300, 846, 1077
MboI GATC 4 cut(s) 187, 298, 844, 1075
MboII GAAGA 6 cut(s) 3, 6, 458, 702, 726, 839
MfeI CAATTG 2 cut(s) 95, 1207
MflI RGATCY 3 cut(s) 298, 844, 1075
MhlI GDGCHC 2 cut(s) 496, 797
MluCI AATT 9 cut(s) 22, 37, 95, 314, 398, 878, 1011, 1029, 1207
MlyI GAGTC 3 cut(s) 872, 917, 1150
MmeI TCCRAC 1 cut(s) 1115
Mph1103I ATGCAT 1 cut(s) 142
MroI TCCGGA 2 cut(s) 61, 990
MseI TTAA 2 cut(s) 50, 1068
MslI CAYNNNNRTG 4 cut(s) 579, 691, 893, 965
MspA1I CMGCKG 2 cut(s) 107, 473
MspI CCGG 3 cut(s) 62, 529, 991
MspR9I CCNGG 4 cut(s) 239, 747, 1073, 1118
MunI CAATTG 2 cut(s) 95, 1207
MvaI CCWGG 4 cut(s) 239, 747, 1073, 1118
MwoI GCNNNNNNNGC 4 cut(s) 83, 476, 1013, 1199
NdeI CATATG 1 cut(s) 161
NdeII GATC 4 cut(s) 187, 298, 844, 1075
NlaIII CATG 5 cut(s) 144, 218, 602, 892, 1148
NlaIV GGNNCC 6 cut(s) 60, 248, 300, 527, 982, 1077
NsiI ATGCAT 1 cut(s) 142
NspI RCATGY 1 cut(s) 144
PceI AGGCCT 1 cut(s) 1174
PciSI GCTCTTC 2 cut(s) 16, 441
PfeI GAWTC 3 cut(s) 683, 820, 1103
PflMI CCANNNNNTGG 1 cut(s) 466
PfoI TCCNGGA 1 cut(s) 237
PinAI ACCGGT 1 cut(s) 528
PleI GAGTC 3 cut(s) 871, 916, 1149
PpsI GAGTC 3 cut(s) 871, 916, 1149
PpuMI RGGWCCY 1 cut(s) 1196
Psp124BI GAGCTC 1 cut(s) 797
Psp5II RGGWCCY 1 cut(s) 1196
Psp6I CCWGG 4 cut(s) 237, 745, 1071, 1116
PspGI CCWGG 4 cut(s) 237, 745, 1071, 1116
PspN4I GGNNCC 6 cut(s) 60, 248, 300, 527, 982, 1077
PspPI GGNCC 4 cut(s) 351, 749, 836, 1196
PspPPI RGGWCCY 1 cut(s) 1196
PstI CTGCAG 2 cut(s) 646, 1195
PstNI CAGNNNCTG 3 cut(s) 110, 476, 1199
PsuI RGATCY 3 cut(s) 298, 844, 1075
PvuII CAGCTG 2 cut(s) 107, 473
RsaI GTAC 2 cut(s) 427, 925
RsaNI GTAC 2 cut(s) 426, 924
RseI CAYNNNNRTG 4 cut(s) 579, 691, 893, 965
SacI GAGCTC 1 cut(s) 797
SapI GCTCTTC 2 cut(s) 16, 441
SaqAI TTAA 2 cut(s) 50, 1068
Sau3AI GATC 4 cut(s) 187, 298, 844, 1075
Sau96I GGNCC 4 cut(s) 351, 749, 836, 1196
SchI GAGTC 3 cut(s) 872, 917, 1150
ScrFI CCNGG 4 cut(s) 239, 747, 1073, 1118
SduI GDGCHC 2 cut(s) 496, 797
SfaNI GCATC 3 cut(s) 127, 243, 424
SfcI CTRYAG 4 cut(s) 154, 642, 1080, 1191
SinI GGWCC 2 cut(s) 836, 1196
SmiMI CAYNNNNRTG 4 cut(s) 579, 691, 893, 965
SmlI CTYRAG 2 cut(s) 167, 1043
SmoI CTYRAG 2 cut(s) 167, 1043
SpeI ACTAGT 1 cut(s) 800
Sse9I AATT 9 cut(s) 22, 37, 95, 314, 398, 878, 1011, 1029, 1207
SseBI AGGCCT 1 cut(s) 1174
SspMI CTAG 3 cut(s) 783, 801, 1017
SstI GAGCTC 1 cut(s) 797
StuI AGGCCT 1 cut(s) 1174
StyD4I CCNGG 4 cut(s) 237, 745, 1071, 1116
StyI CCWWGG 1 cut(s) 391
TaqI TCGA 1 cut(s) 1027
TasI AATT 9 cut(s) 22, 37, 95, 314, 398, 878, 1011, 1029, 1207
TatI WGTACW 1 cut(s) 923
TfiI GAWTC 3 cut(s) 683, 820, 1103
Tru1I TTAA 2 cut(s) 50, 1068
Tru9I TTAA 2 cut(s) 50, 1068
TscAI CASTG 2 cut(s) 201, 236
TspDTI ATGAA 4 cut(s) 17, 696, 833, 911
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 2 cut(s) 201, 236
Van91I CCANNNNNTGG 1 cut(s) 466
VpaK11BI GGWCC 2 cut(s) 836, 1196
XapI RAATTY 3 cut(s) 22, 398, 878
XceI RCATGY 1 cut(s) 144
XspI CTAG 3 cut(s) 783, 801, 1017
Zsp2I ATGCAT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.