FvH4_6g22320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014496
Physical Location & Seq
Reverse (-)
15971234 .. 15973071
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22320.t1

Sequence Viewer

Length: 459 bp
ATGGACCCTCCTACTCTTACTCCTATTCTTACTACTGCTGCTGCTGCTGCTGCTGTTCAACATGTGACCAAGAGGTCTTCGGATGAGCTGCTCAGGAAATTTGCTGATTCCTGCAATGAGGCCGAGGCCTCAGCAAAGAAGAAGGCGATCAGGGCGCTTTCAAAACAAAGGAAAAGAAGCCGGAAAAGCGGTGAGTTTGATCAGAGTGAGAGTCTATCAGATGGCAAGGGTAGCAGCTTAGTGGAGAGGAGGTCACTGCTTCCTGTTGTTGCTGGGACTAAAAACAAGGGATTGCTGCTTAGGCAGCTTGGGGTTCATGGCAGGTCAAGGGATATCAGGAACAAGTCCCTCTTTGGTACCATTCACAAGACTTGGAGGAGGACCATAGAAGGGGCGTCAAAGGTTTTCATGGAGAAGCATTACAACAGGCACAGGCGTTTGATAAATGACATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.99

Weight (kDa)

10.83

Isoelectric Point (pI)

67.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42110
fragaria_vesca FvH4_6g22320
malus_domestica MD12G1057100.v1.1 MD14G1057700.v1.1
prunus_persica Prupe.7G077000_v2.0.a1
pyrus_communis pycom12g05100 pycom14g04680
rosa_chinensis RchiOBHm_Chr3g0479681
rosa_laevigata RLG00000023548
rosa_multiflora Rmu_sc0003755.1_g000005
rosa_roxburghii Rroxscaffold_6G00402070
rosa_rugosa Rorug03G0177400
rosa_samantha Rh3AG228100 Rh3BG261300 Rh3CG258400 Rh3DG254600
rosa_wichuraiana Rw3G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 73
Acc36I ACCTGC 1 cut(s) 312
Acc65I GGTACC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 356
AciI CCGC 1 cut(s) 189
AcsI RAATTY 1 cut(s) 98
AcyI GRCGYC 1 cut(s) 395
AfaI GTAC 1 cut(s) 358
AfiI CCNNNNNNNGG 1 cut(s) 390
AflIII ACRYGT 1 cut(s) 61
AgsI TTSAA 2 cut(s) 59, 162
AluBI AGCT 3 cut(s) 88, 237, 307
AluI AGCT 3 cut(s) 88, 237, 307
AoxI GGCC 2 cut(s) 120, 126
ApeKI GCWGC 9 cut(s) 38, 41, 44, 47, 50, 88, 234, 295, 304
ApoI RAATTY 1 cut(s) 98
Asp718I GGTACC 1 cut(s) 356
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 4, 381
AsuHPI GGTGA 1 cut(s) 203
AvaII GGWCC 2 cut(s) 4, 381
BanI GGYRCC 1 cut(s) 356
BbsI GAAGAC 1 cut(s) 69
BbvCI CCTCAGC 1 cut(s) 130
BbvI GCAGC 9 cut(s) 25, 28, 31, 34, 37, 75, 246, 282, 316
BccI CCATC 1 cut(s) 215
BclI TGATCA 1 cut(s) 199
BfoI RGCGCY 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 312
BisI GCNGC 9 cut(s) 39, 42, 45, 48, 51, 89, 235, 296, 305
BlsI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 90, 236, 297, 306
Bme18I GGWCC 2 cut(s) 4, 381
BmgT120I GGNCC 2 cut(s) 4, 381
BmiI GGNNCC 2 cut(s) 6, 358
BpiI GAAGAC 1 cut(s) 69
Bpu10I CCTNAGC 3 cut(s) 92, 130, 299
BsaHI GRCGYC 1 cut(s) 395
BsaJI CCNNGG 1 cut(s) 123
BsaXI ACNNNNNCTCC 3 cut(s) 34, 236, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 390
Bse3DI GCAATG 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 390
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 2 cut(s) 106, 144
BseRI GAGGAG 2 cut(s) 262, 391
BseXI GCAGC 9 cut(s) 25, 28, 31, 34, 37, 75, 246, 282, 316
BseYI CCCAGC 1 cut(s) 272
BshFI GGCC 2 cut(s) 122, 128
BshNI GGYRCC 1 cut(s) 356
BsiSI CCGG 1 cut(s) 181
BslFI GGGAC 2 cut(s) 289, 331
BslI CCNNNNNNNGG 1 cut(s) 390
BsmFI GGGAC 2 cut(s) 289, 331
BsnI GGCC 2 cut(s) 122, 128
Bsp143I GATC 2 cut(s) 147, 199
BspACI CCGC 1 cut(s) 189
BspANI GGCC 2 cut(s) 122, 128
BspCNI CTCAG 2 cut(s) 105, 143
BspLI GGNNCC 2 cut(s) 6, 358
BspMI ACCTGC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 2 cut(s) 147, 199
BssNI GRCGYC 1 cut(s) 395
BstACI GRCGYC 1 cut(s) 395
BstDEI CTNAG 4 cut(s) 92, 130, 238, 299
BstF5I GGATG 1 cut(s) 88
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 2 cut(s) 150, 202
BstMBI GATC 2 cut(s) 147, 199
BstMWI GCNNNNNNNGC 8 cut(s) 44, 47, 50, 152, 186, 231, 301, 304
BstNSI RCATGY 1 cut(s) 65
BstV1I GCAGC 9 cut(s) 25, 28, 31, 34, 37, 75, 246, 282, 316
BstV2I GAAGAC 1 cut(s) 69
BsuRI GGCC 2 cut(s) 122, 128
BtsCI GGATG 1 cut(s) 88
BtsI GCAGTG 1 cut(s) 254
BtsIMutI CAGTG 1 cut(s) 254
BveI ACCTGC 1 cut(s) 312
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 4, 381
CseI GACGC 1 cut(s) 384
Csp6I GTAC 1 cut(s) 357
CviAII CATG 3 cut(s) 62, 317, 409
CviJI RGCY 6 cut(s) 88, 122, 128, 180, 237, 307
CviKI_1 RGCY 6 cut(s) 88, 122, 128, 180, 237, 307
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 4 cut(s) 92, 130, 238, 299
DpnI GATC 2 cut(s) 149, 201
DpnII GATC 2 cut(s) 147, 199
DrdI GACNNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 73
Eco147I AGGCCT 1 cut(s) 128
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 2 cut(s) 4, 381
EcoRV GATATC 1 cut(s) 334
FaeI CATG 3 cut(s) 65, 320, 412
FaiI YATR 4 cut(s) 63, 318, 386, 410
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 2 cut(s) 289, 331
FatI CATG 3 cut(s) 61, 316, 408
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 9 cut(s) 39, 42, 45, 48, 51, 89, 235, 296, 305
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 9 cut(s) 39, 42, 45, 48, 51, 89, 235, 296, 305
GlaI GCGC 1 cut(s) 156
GluI GCNGC 9 cut(s) 39, 42, 45, 48, 51, 89, 235, 296, 305
GsaI CCCAGC 1 cut(s) 276
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 2 cut(s) 122, 128
HapII CCGG 1 cut(s) 181
HgaI GACGC 1 cut(s) 384
HhaI GCGC 1 cut(s) 157
Hin1I GRCGYC 1 cut(s) 395
Hin1II CATG 3 cut(s) 65, 320, 412
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HinfI GANTC 2 cut(s) 107, 211
HpaII CCGG 1 cut(s) 181
HphI GGTGA 1 cut(s) 203
Hpy188I TCNGA 3 cut(s) 82, 204, 220
Hpy188III TCNNGA 2 cut(s) 94, 337
HpyAV CCTTC 2 cut(s) 136, 383
HpyCH4V TGCA 1 cut(s) 114
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 47, 50, 152, 186, 231, 301, 304
HpyF3I CTNAG 4 cut(s) 92, 130, 238, 299
Hsp92I GRCGYC 1 cut(s) 395
Hsp92II CATG 3 cut(s) 65, 320, 412
HspAI GCGC 1 cut(s) 155
KpnI GGTACC 1 cut(s) 360
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 2 cut(s) 147, 199
Lsp1109I GCAGC 9 cut(s) 25, 28, 31, 34, 37, 75, 246, 282, 316
MaeIII GTNAC 2 cut(s) 64, 252
MalI GATC 2 cut(s) 149, 201
MboI GATC 2 cut(s) 147, 199
MboII GAAGA 2 cut(s) 69, 151
MluCI AATT 1 cut(s) 98
MlyI GAGTC 1 cut(s) 220
MspI CCGG 1 cut(s) 181
MwoI GCNNNNNNNGC 8 cut(s) 44, 47, 50, 152, 186, 231, 301, 304
NdeII GATC 2 cut(s) 147, 199
NlaIII CATG 3 cut(s) 65, 320, 412
NlaIV GGNNCC 2 cut(s) 6, 358
NmeAIII GCCGAG 1 cut(s) 148
NmuCI GTSAC 2 cut(s) 64, 252
NspI RCATGY 1 cut(s) 65
PceI AGGCCT 1 cut(s) 128
PciI ACATGT 1 cut(s) 61
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 90, 236, 297, 306
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
PscI ACATGT 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 272
PspN4I GGNNCC 2 cut(s) 6, 358
PspPI GGNCC 2 cut(s) 4, 381
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
SatI GCNGC 9 cut(s) 39, 42, 45, 48, 51, 89, 235, 296, 305
Sau3AI GATC 2 cut(s) 147, 199
Sau96I GGNCC 2 cut(s) 4, 381
SchI GAGTC 1 cut(s) 220
SetI ASST 7 cut(s) 77, 90, 239, 254, 309, 326, 405
SinI GGWCC 2 cut(s) 4, 381
Sse9I AATT 1 cut(s) 98
SseBI AGGCCT 1 cut(s) 128
SsiI CCGC 1 cut(s) 189
StuI AGGCCT 1 cut(s) 128
TasI AATT 1 cut(s) 98
TfiI GAWTC 1 cut(s) 107
TscAI CASTG 1 cut(s) 261
TseFI GTSAC 2 cut(s) 64, 252
TseI GCWGC 9 cut(s) 38, 41, 44, 47, 50, 88, 234, 295, 304
Tsp45I GTSAC 2 cut(s) 64, 252
TspDTI ATGAA 2 cut(s) 305, 397
TspRI CASTG 1 cut(s) 261
VpaK11BI GGWCC 2 cut(s) 4, 381
XapI RAATTY 1 cut(s) 98
XceI RCATGY 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.