Rh3DG254600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0014496
Physical Location & Seq
Reverse (-)
25203443 .. 25205454
2012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG254600.1

Sequence Viewer

Length: 486 bp
ATGGACCCTCCTACTTCTATTCCTACTTCTGTTGCTGCTACTGCTACTGCTACTGCTACTGCTACTGCTACTGCTACTGCCTCTGCTAGTGTTCAACATGTGACCAAGAGGTCTTCAGATGAGCTGCTCAGGAAATTTGCTGATTCCTGCAATGAGGCCGAGGCCTCAGCGAGGAAGAAGGCGATCAGGGAGCTTTCGAAGCAAAGGAAAAAAAGCCGGAAAAGTGGTGAGTTTGATCAGGGTGAGAGTCCATCAAATGGCAAGAGTAGCAGCTTAGTGGAGAGGAGGTCACTGCTTCCTGTTGTTGCTGGGACTAAAAACAAGGGATTGCTGCTTAGGCAGCTTGGGGTTCATGGCAGGTCAAGGGATATCAGGAACAAGTCCCTTTTTGGTACCATTCACAAGACTTGGAGGAGGACCATAGAAGGGGCTTCAAAGATCTGGAGATTTGGAAATCTCTACAATCTCATCCTCCCAATAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

17.52

Weight (kDa)

10.83

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42110
fragaria_vesca FvH4_6g22320
malus_domestica MD12G1057100.v1.1 MD14G1057700.v1.1
prunus_persica Prupe.7G077000_v2.0.a1
pyrus_communis pycom12g05100 pycom14g04680
rosa_chinensis RchiOBHm_Chr3g0479681
rosa_laevigata RLG00000023548
rosa_multiflora Rmu_sc0003755.1_g000005
rosa_roxburghii Rroxscaffold_6G00402070
rosa_rugosa Rorug03G0177400
rosa_samantha Rh3AG228100 Rh3BG261300 Rh3CG258400 Rh3DG254600
rosa_wichuraiana Rw3G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 109
Acc36I ACCTGC 1 cut(s) 348
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 392
AccB7I CCANNNNNTGG 1 cut(s) 257
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 99
AfaI GTAC 1 cut(s) 394
AfiI CCNNNNNNNGG 3 cut(s) 171, 257, 426
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 2 cut(s) 95, 435
AluBI AGCT 4 cut(s) 124, 193, 273, 343
AluI AGCT 4 cut(s) 124, 193, 273, 343
AoxI GGCC 2 cut(s) 156, 162
ApeKI GCWGC 5 cut(s) 35, 124, 270, 331, 340
ApoI RAATTY 1 cut(s) 134
Asp718I GGTACC 1 cut(s) 392
AspS9I GGNCC 2 cut(s) 4, 417
AsuHPI GGTGA 2 cut(s) 239, 254
AsuII TTCGAA 1 cut(s) 197
AvaII GGWCC 2 cut(s) 4, 417
BanI GGYRCC 1 cut(s) 392
BbsI GAAGAC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 166
BbvI GCAGC 5 cut(s) 22, 111, 282, 318, 352
BccI CCATC 1 cut(s) 259
BclI TGATCA 1 cut(s) 235
BfaI CTAG 1 cut(s) 87
BfuAI ACCTGC 1 cut(s) 348
BglII AGATCT 1 cut(s) 438
BisI GCNGC 5 cut(s) 36, 125, 271, 332, 341
BlsI GCNGC 5 cut(s) 37, 126, 272, 333, 342
Bme18I GGWCC 2 cut(s) 4, 417
BmgT120I GGNCC 2 cut(s) 4, 417
BmiI GGNNCC 2 cut(s) 6, 394
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 1 cut(s) 463
Bpu10I CCTNAGC 3 cut(s) 128, 166, 335
Bpu14I TTCGAA 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 257, 426
Bse3DI GCAATG 1 cut(s) 157
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 1 cut(s) 468
BseLI CCNNNNNNNGG 3 cut(s) 171, 257, 426
BseMI GCAATG 1 cut(s) 157
BseMII CTCAG 2 cut(s) 142, 180
BseRI GAGGAG 2 cut(s) 298, 427
BseXI GCAGC 5 cut(s) 22, 111, 282, 318, 352
BseYI CCCAGC 1 cut(s) 308
BshFI GGCC 2 cut(s) 158, 164
BshNI GGYRCC 1 cut(s) 392
BsiSI CCGG 1 cut(s) 217
BslFI GGGAC 2 cut(s) 325, 367
BslI CCNNNNNNNGG 3 cut(s) 171, 257, 426
BsmFI GGGAC 2 cut(s) 325, 367
BsnI GGCC 2 cut(s) 158, 164
Bsp119I TTCGAA 1 cut(s) 197
Bsp143I GATC 3 cut(s) 183, 235, 438
BspANI GGCC 2 cut(s) 158, 164
BspCNI CTCAG 2 cut(s) 141, 179
BspLI GGNNCC 2 cut(s) 6, 394
BspMI ACCTGC 1 cut(s) 348
BspT104I TTCGAA 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 157
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 3 cut(s) 183, 235, 438
BstBI TTCGAA 1 cut(s) 197
BstDEI CTNAG 4 cut(s) 128, 166, 274, 335
BstENI CCTNNNNNAGG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 468
BstKTI GATC 3 cut(s) 186, 238, 441
BstMBI GATC 3 cut(s) 183, 235, 438
BstMWI GCNNNNNNNGC 5 cut(s) 41, 199, 267, 337, 340
BstNSI RCATGY 1 cut(s) 101
BstV1I GCAGC 5 cut(s) 22, 111, 282, 318, 352
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BsuRI GGCC 2 cut(s) 158, 164
BtsCI GGATG 1 cut(s) 468
BtsI GCAGTG 1 cut(s) 290
BtsIMutI CAGTG 1 cut(s) 290
BveI ACCTGC 1 cut(s) 348
Cfr13I GGNCC 2 cut(s) 4, 417
Csp6I GTAC 1 cut(s) 393
CviAII CATG 2 cut(s) 98, 353
CviJI RGCY 8 cut(s) 124, 158, 164, 193, 216, 273, 343, 431
CviKI_1 RGCY 8 cut(s) 124, 158, 164, 193, 216, 273, 343, 431
CviQI GTAC 1 cut(s) 393
DdeI CTNAG 4 cut(s) 128, 166, 274, 335
DpnI GATC 3 cut(s) 185, 237, 440
DpnII GATC 3 cut(s) 183, 235, 438
DrdI GACNNNNNNGTC 1 cut(s) 109
DseDI GACNNNNNNGTC 1 cut(s) 109
Eco147I AGGCCT 1 cut(s) 164
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 2 cut(s) 4, 417
Eco57I CTGAAG 1 cut(s) 99
EcoNI CCTNNNNNAGG 1 cut(s) 169
EcoRV GATATC 1 cut(s) 370
FaeI CATG 2 cut(s) 101, 356
FaiI YATR 3 cut(s) 99, 354, 422
FaqI GGGAC 2 cut(s) 325, 367
FatI CATG 2 cut(s) 97, 352
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 5 cut(s) 36, 125, 271, 332, 341
FokI GGATG 1 cut(s) 455
Fsp4HI GCNGC 5 cut(s) 36, 125, 271, 332, 341
FspBI CTAG 1 cut(s) 87
GluI GCNGC 5 cut(s) 36, 125, 271, 332, 341
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 1 cut(s) 463
HaeIII GGCC 2 cut(s) 158, 164
HapII CCGG 1 cut(s) 217
Hin1II CATG 2 cut(s) 101, 356
HinfI GANTC 2 cut(s) 143, 247
HpaII CCGG 1 cut(s) 217
HphI GGTGA 2 cut(s) 239, 254
Hpy188I TCNGA 1 cut(s) 118
Hpy188III TCNNGA 3 cut(s) 130, 373, 442
HpyAV CCTTC 2 cut(s) 172, 419
HpyCH4V TGCA 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 199, 267, 337, 340
HpyF3I CTNAG 4 cut(s) 128, 166, 274, 335
Hsp92II CATG 2 cut(s) 101, 356
KpnI GGTACC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 3 cut(s) 183, 235, 438
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 5 cut(s) 22, 111, 282, 318, 352
MaeI CTAG 1 cut(s) 87
MaeIII GTNAC 2 cut(s) 100, 288
MalI GATC 3 cut(s) 185, 237, 440
MboI GATC 3 cut(s) 183, 235, 438
MboII GAAGA 2 cut(s) 105, 187
MflI RGATCY 1 cut(s) 438
MluCI AATT 1 cut(s) 134
MlyI GAGTC 1 cut(s) 256
MspI CCGG 1 cut(s) 217
MwoI GCNNNNNNNGC 5 cut(s) 41, 199, 267, 337, 340
NdeII GATC 3 cut(s) 183, 235, 438
NlaIII CATG 2 cut(s) 101, 356
NlaIV GGNNCC 2 cut(s) 6, 394
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 2 cut(s) 100, 288
NspI RCATGY 1 cut(s) 101
NspV TTCGAA 1 cut(s) 197
PceI AGGCCT 1 cut(s) 164
PciI ACATGT 1 cut(s) 97
PfeI GAWTC 1 cut(s) 143
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 5 cut(s) 37, 126, 272, 333, 342
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PscI ACATGT 1 cut(s) 97
PspFI CCCAGC 1 cut(s) 308
PspN4I GGNNCC 2 cut(s) 6, 394
PspPI GGNCC 2 cut(s) 4, 417
PsuI RGATCY 1 cut(s) 438
RsaI GTAC 1 cut(s) 394
RsaNI GTAC 1 cut(s) 393
SatI GCNGC 5 cut(s) 36, 125, 271, 332, 341
Sau3AI GATC 3 cut(s) 183, 235, 438
Sau96I GGNCC 2 cut(s) 4, 417
SchI GAGTC 1 cut(s) 256
SetI ASST 7 cut(s) 113, 126, 195, 275, 290, 345, 362
SfuI TTCGAA 1 cut(s) 197
SinI GGWCC 2 cut(s) 4, 417
Sse9I AATT 1 cut(s) 134
SseBI AGGCCT 1 cut(s) 164
SspMI CTAG 1 cut(s) 87
StuI AGGCCT 1 cut(s) 164
TaqI TCGA 1 cut(s) 197
TasI AATT 1 cut(s) 134
TfiI GAWTC 1 cut(s) 143
TscAI CASTG 1 cut(s) 297
TseFI GTSAC 2 cut(s) 100, 288
TseI GCWGC 5 cut(s) 35, 124, 270, 331, 340
Tsp45I GTSAC 2 cut(s) 100, 288
TspDTI ATGAA 1 cut(s) 341
TspRI CASTG 1 cut(s) 297
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 4, 417
XagI CCTNNNNNAGG 1 cut(s) 169
XapI RAATTY 1 cut(s) 134
XceI RCATGY 1 cut(s) 101
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.