pycom12g05100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014496
Physical Location & Seq
Reverse (-)
4973758 .. 4974839
1082 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05100.2

Sequence Viewer

Length: 459 bp
ATGGAATCCCTCACTACTGCAACTCCTACTACTCGTGCTAGTGTTCAGCATGTGACCAAGAAGTCTTCAGACGAGCTGCTGAGGAAATTTGCTGATTCCGGGGACGAGGCCGATGCCCCGGCGAGGAAACAGCTGGCGGTGAGGGTGTCGAAGCGCCGGAAAAGAAGCCGGGTGAACGGGGAAGGGGATCATCAGTGCGATAGTCCGTCGAACGGGAGGAGTACTGGATTGGTGGAGAGGAGGTCTCTGCTTCCTGCTGGGACTAAAAACAAGGCCTTGTTTAGGAAAATTGGGATTCATGGCGGCAGGGGCTCACACCTCAGGGCGACGGATATCAGGAACAAGTCCCTCTTCGGTTCAATTCGCAAGACATGGAGAAGGACCATTGAAGGAGCTTCAAAGGTTTTCATGGAAAAGCATTACAATAGGCACAAGCGTTTGATCAATGACATTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.06

Weight (kDa)

11.22

Isoelectric Point (pI)

60.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42110
fragaria_vesca FvH4_6g22320
malus_domestica MD12G1057100.v1.1 MD14G1057700.v1.1
prunus_persica Prupe.7G077000_v2.0.a1
pyrus_communis pycom12g05100 pycom14g04680
rosa_chinensis RchiOBHm_Chr3g0479681
rosa_laevigata RLG00000023548
rosa_multiflora Rmu_sc0003755.1_g000005
rosa_roxburghii Rroxscaffold_6G00402070
rosa_rugosa Rorug03G0177400
rosa_samantha Rh3AG228100 Rh3BG261300 Rh3CG258400 Rh3DG254600
rosa_wichuraiana Rw3G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
AciI CCGC 2 cut(s) 137, 303
AclWI GGATC 1 cut(s) 195
AcsI RAATTY 1 cut(s) 86
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 1 cut(s) 223
AfiI CCNNNNNNNGG 3 cut(s) 123, 212, 282
AgsI TTSAA 3 cut(s) 360, 389, 399
AluBI AGCT 3 cut(s) 76, 133, 395
AluI AGCT 3 cut(s) 76, 133, 395
Alw26I GTCTC 1 cut(s) 249
AlwI GGATC 1 cut(s) 195
AoxI GGCC 2 cut(s) 108, 273
ApeKI GCWGC 1 cut(s) 76
ApoI RAATTY 1 cut(s) 86
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 381
AsuC2I CCSGG 3 cut(s) 100, 119, 170
AsuHPI GGTGA 2 cut(s) 151, 184
AvaII GGWCC 1 cut(s) 381
AxyI CCTNAGG 1 cut(s) 320
BanII GRGCYC 1 cut(s) 314
BauI CACGAG 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 57
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 63
BcgI CGANNNNNNTGC 2 cut(s) 95, 129
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 3 cut(s) 100, 119, 170
BcoDI GTCTC 1 cut(s) 249
BfaI CTAG 2 cut(s) 39, 457
BfoI RGCGCY 1 cut(s) 157
BisI GCNGC 2 cut(s) 77, 304
BlsI GCNGC 2 cut(s) 78, 305
BmcAI AGTACT 1 cut(s) 223
Bme1390I CCNGG 3 cut(s) 100, 119, 170
Bme18I GGWCC 1 cut(s) 381
BmgT120I GGNCC 1 cut(s) 381
BmrFI CCNGG 3 cut(s) 100, 119, 170
BmsI GCATC 1 cut(s) 103
BpiI GAAGAC 1 cut(s) 57
BplI GAGNNNNNCTC 2 cut(s) 229, 261
Bpu10I CCTNAGC 1 cut(s) 80
BpuMI CCSGG 3 cut(s) 100, 119, 170
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 2 cut(s) 99, 117
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 227, 257
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 212, 282
Bse1I ACTGG 1 cut(s) 229
Bse21I CCTNAGG 1 cut(s) 320
BseDI CCNNGG 2 cut(s) 99, 117
BseLI CCNNNNNNNGG 3 cut(s) 123, 212, 282
BseMII CTCAG 2 cut(s) 71, 334
BseNI ACTGG 1 cut(s) 229
BseRI GAGGAG 2 cut(s) 232, 253
BseXI GCAGC 1 cut(s) 63
BseYI CCCAGC 1 cut(s) 257
BshFI GGCC 2 cut(s) 110, 275
BsiSI CCGG 4 cut(s) 99, 119, 157, 169
BslFI GGGAC 3 cut(s) 116, 274, 331
BslI CCNNNNNNNGG 3 cut(s) 123, 212, 282
BsmAI GTCTC 1 cut(s) 249
BsmFI GGGAC 3 cut(s) 116, 274, 331
BsnI GGCC 2 cut(s) 110, 275
Bso31I GGTCTC 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 2 cut(s) 187, 441
BspACI CCGC 2 cut(s) 137, 303
BspANI GGCC 2 cut(s) 110, 275
BspCNI CTCAG 2 cut(s) 72, 333
BspPI GGATC 1 cut(s) 195
BspTNI GGTCTC 1 cut(s) 249
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 99, 117
BssMI GATC 2 cut(s) 187, 441
BssSI CACGAG 1 cut(s) 33
Bst2BI CACGAG 1 cut(s) 33
Bst6I CTCTTC 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 2 cut(s) 80, 320
BstENI CCTNNNNNAGG 1 cut(s) 280
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 2 cut(s) 190, 444
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 2 cut(s) 187, 441
BstMWI GCNNNNNNNGC 1 cut(s) 309
BstNSI RCATGY 1 cut(s) 53
BstSCI CCNGG 3 cut(s) 98, 117, 168
BstV1I GCAGC 1 cut(s) 63
BstV2I GAAGAC 1 cut(s) 57
Bsu36I CCTNAGG 1 cut(s) 320
BsuRI GGCC 2 cut(s) 110, 275
BtsIMutI CAGTG 1 cut(s) 200
Cac8I GCNNGC 1 cut(s) 135
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 381
Csp6I GTAC 1 cut(s) 222
CviAII CATG 4 cut(s) 50, 299, 372, 409
CviJI RGCY 7 cut(s) 76, 110, 133, 168, 275, 312, 395
CviKI_1 RGCY 7 cut(s) 76, 110, 133, 168, 275, 312, 395
CviQI GTAC 1 cut(s) 222
DdeI CTNAG 2 cut(s) 80, 320
DpnI GATC 2 cut(s) 189, 443
DpnII GATC 2 cut(s) 187, 441
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
Eco147I AGGCCT 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 314
Eco31I GGTCTC 1 cut(s) 249
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 381
Eco57I CTGAAG 1 cut(s) 51
Eco81I CCTNAGG 1 cut(s) 320
EcoNI CCTNNNNNAGG 1 cut(s) 280
EcoRV GATATC 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 314
FaeI CATG 4 cut(s) 53, 302, 375, 412
FaiI YATR 4 cut(s) 51, 300, 373, 410
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 3 cut(s) 116, 274, 331
FatI CATG 4 cut(s) 49, 298, 371, 408
FbaI TGATCA 1 cut(s) 441
Fnu4HI GCNGC 2 cut(s) 77, 304
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 2 cut(s) 77, 304
FspBI CTAG 2 cut(s) 39, 457
GlaI GCGC 1 cut(s) 155
GluI GCNGC 2 cut(s) 77, 304
GsaI CCCAGC 1 cut(s) 261
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 2 cut(s) 110, 275
HapII CCGG 4 cut(s) 99, 119, 157, 169
HhaI GCGC 1 cut(s) 156
Hin1II CATG 4 cut(s) 53, 302, 375, 412
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HinfI GANTC 3 cut(s) 5, 95, 295
HpaII CCGG 4 cut(s) 99, 119, 157, 169
HphI GGTGA 2 cut(s) 151, 184
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 1 cut(s) 337
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 2 cut(s) 211, 331
HpyAV CCTTC 3 cut(s) 176, 372, 383
HpyCH4V TGCA 1 cut(s) 20
HpyF10VI GCNNNNNNNGC 1 cut(s) 309
HpyF3I CTNAG 2 cut(s) 80, 320
Hsp92II CATG 4 cut(s) 53, 302, 375, 412
HspAI GCGC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 2 cut(s) 187, 441
LmnI GCTCC 1 cut(s) 392
Lsp1109I GCAGC 1 cut(s) 63
LweI GCATC 1 cut(s) 103
MaeI CTAG 2 cut(s) 39, 457
MaeIII GTNAC 1 cut(s) 52
MalI GATC 2 cut(s) 189, 443
MboI GATC 2 cut(s) 187, 441
MboII GAAGA 2 cut(s) 57, 343
MhlI GDGCHC 1 cut(s) 314
MluCI AATT 3 cut(s) 86, 288, 360
MspA1I CMGCKG 1 cut(s) 133
MspI CCGG 4 cut(s) 99, 119, 157, 169
MspR9I CCNGG 3 cut(s) 100, 119, 170
MwoI GCNNNNNNNGC 1 cut(s) 309
NciI CCSGG 3 cut(s) 100, 119, 170
NdeII GATC 2 cut(s) 187, 441
NlaIII CATG 4 cut(s) 53, 302, 375, 412
NmuCI GTSAC 1 cut(s) 52
NspI RCATGY 1 cut(s) 53
PceI AGGCCT 1 cut(s) 275
PfeI GAWTC 3 cut(s) 5, 95, 295
PflFI GACNNNGTC 1 cut(s) 452
PkrI GCNGC 2 cut(s) 78, 305
PspFI CCCAGC 1 cut(s) 257
PspPI GGNCC 1 cut(s) 381
PsyI GACNNNGTC 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 133
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
SatI GCNGC 2 cut(s) 77, 304
Sau3AI GATC 2 cut(s) 187, 441
Sau96I GGNCC 1 cut(s) 381
ScaI AGTACT 1 cut(s) 223
ScrFI CCNGG 3 cut(s) 100, 119, 170
SduI GDGCHC 1 cut(s) 314
SetI ASST 6 cut(s) 78, 135, 245, 321, 397, 405
SfaNI GCATC 1 cut(s) 103
SinI GGWCC 1 cut(s) 381
Sse9I AATT 3 cut(s) 86, 288, 360
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 2 cut(s) 137, 303
SspMI CTAG 2 cut(s) 39, 457
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 3 cut(s) 98, 117, 168
TaqI TCGA 2 cut(s) 149, 209
TasI AATT 3 cut(s) 86, 288, 360
TatI WGTACW 1 cut(s) 221
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 3 cut(s) 5, 95, 295
TscAI CASTG 1 cut(s) 200
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 76
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 287, 397
TspGWI ACGGA 2 cut(s) 195, 344
TspRI CASTG 1 cut(s) 200
Tth111I GACNNNGTC 1 cut(s) 452
VpaK11BI GGWCC 1 cut(s) 381
XagI CCTNNNNNAGG 1 cut(s) 280
XapI RAATTY 1 cut(s) 86
XceI RCATGY 1 cut(s) 53
XspI CTAG 2 cut(s) 39, 457
ZrmI AGTACT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.