MD14G1057700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014496
Physical Location & Seq
Reverse (-)
5955668 .. 5957746
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1057700.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGAAGCTATCACTACGACTACTCCTTGTACTGCTGCTGCTGCTGCTGCTGCTGCTGCATCCGCAAGTGTTCAACATGTGACCAAGAAGTCTTCCGACGAGCTGCTGAGGAAATTTGCGGATTCCGGGGACGAGGCTGAGGTTCCTGCGAGGAAACAGCTGGCGGTGAGGGTGTCGAAGCGTCGGAAAAGAAGCCGGGTGAACGGGGATGGGGATCAGCAGTGTGAGAGCCCGTTGAGTGGGAAGAGTGGGTTGGTGGAGAGGAGGTCTCTGCTTCCTGCTGGGACTAAAAGCAAGGCGTTGTTTAGGAAAATTGGGATTCATGGCGGCAGGGCTTCACACCTCAGGGCTAGGGATATCAGGAACAAGTCCCTCTTTGGCACAATTCACAAGACATGGAGAATGGCCATTGAAGGAGCTTCAAAGGTTTTCATGGAAAAGCATTACAACAGGCACAAGCGTTTGATCAATGACATTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

17.53

Weight (kDa)

10.68

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42110
fragaria_vesca FvH4_6g22320
malus_domestica MD12G1057100.v1.1 MD14G1057700.v1.1
prunus_persica Prupe.7G077000_v2.0.a1
pyrus_communis pycom12g05100 pycom14g04680
rosa_chinensis RchiOBHm_Chr3g0479681
rosa_laevigata RLG00000023548
rosa_multiflora Rmu_sc0003755.1_g000005
rosa_roxburghii Rroxscaffold_6G00402070
rosa_rugosa Rorug03G0177400
rosa_samantha Rh3AG228100 Rh3BG261300 Rh3CG258400 Rh3DG254600
rosa_wichuraiana Rw3G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
AciI CCGC 4 cut(s) 63, 119, 164, 327
AclWI GGATC 1 cut(s) 222
AcoI YGGCCR 1 cut(s) 405
AcsI RAATTY 1 cut(s) 113
AfaI GTAC 1 cut(s) 31
AfiI CCNNNNNNNGG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 3 cut(s) 74, 413, 423
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 4 cut(s) 8, 103, 160, 419
AluI AGCT 4 cut(s) 8, 103, 160, 419
Alw26I GTCTC 1 cut(s) 273
AlwI GGATC 1 cut(s) 222
AoxI GGCC 1 cut(s) 405
ApeKI GCWGC 9 cut(s) 35, 38, 41, 44, 47, 50, 53, 56, 103
ApoI RAATTY 1 cut(s) 113
AsuC2I CCSGG 2 cut(s) 127, 197
AsuHPI GGTGA 2 cut(s) 178, 211
AxyI CCTNAGG 1 cut(s) 344
BalI TGGCCA 1 cut(s) 407
BanII GRGCYC 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 84
BbvCI CCTCAGC 2 cut(s) 107, 138
BbvI GCAGC 9 cut(s) 22, 25, 28, 31, 34, 37, 40, 43, 90
BccI CCATC 1 cut(s) 203
BclI TGATCA 1 cut(s) 465
BcnI CCSGG 2 cut(s) 127, 197
BcoDI GTCTC 1 cut(s) 273
BfaI CTAG 2 cut(s) 351, 481
Bme1390I CCNGG 2 cut(s) 127, 197
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 2 cut(s) 127, 197
BmsI GCATC 1 cut(s) 68
BpiI GAAGAC 1 cut(s) 84
BplI GAGNNNNNCTC 2 cut(s) 253, 285
Bpu10I CCTNAGC 2 cut(s) 107, 138
BpuMI CCSGG 2 cut(s) 127, 197
BsaBI GATNNNNATC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 126
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 251, 281
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse21I CCTNAGG 1 cut(s) 344
Bse8I GATNNNNATC 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 2 cut(s) 59, 214
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMII CTCAG 3 cut(s) 98, 129, 358
BseRI GAGGAG 1 cut(s) 277
BseXI GCAGC 9 cut(s) 22, 25, 28, 31, 34, 37, 40, 43, 90
BseYI CCCAGC 1 cut(s) 281
BshFI GGCC 1 cut(s) 407
BsiSI CCGG 2 cut(s) 126, 196
BslFI GGGAC 3 cut(s) 143, 298, 355
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 1 cut(s) 273
BsmFI GGGAC 3 cut(s) 143, 298, 355
BsnI GGCC 1 cut(s) 407
Bso31I GGTCTC 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 2 cut(s) 214, 465
BspACI CCGC 4 cut(s) 63, 119, 164, 327
BspANI GGCC 1 cut(s) 407
BspCNI CTCAG 3 cut(s) 99, 130, 357
BspLI GGNNCC 1 cut(s) 144
BspPI GGATC 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 273
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 2 cut(s) 214, 465
Bst6I CTCTTC 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 3 cut(s) 107, 138, 344
BstF5I GGATG 2 cut(s) 59, 214
BstKTI GATC 2 cut(s) 217, 468
BstMAI GTCTC 1 cut(s) 273
BstMBI GATC 2 cut(s) 214, 465
BstMWI GCNNNNNNNGC 7 cut(s) 41, 44, 47, 50, 53, 56, 62
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 125, 195
BstV1I GCAGC 9 cut(s) 22, 25, 28, 31, 34, 37, 40, 43, 90
BstV2I GAAGAC 1 cut(s) 84
Bsu36I CCTNAGG 1 cut(s) 344
BsuRI GGCC 1 cut(s) 407
BtsCI GGATG 2 cut(s) 59, 214
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 1 cut(s) 227
Cac8I GCNNGC 1 cut(s) 162
CseI GACGC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 30
CviAII CATG 4 cut(s) 77, 323, 396, 433
CviQI GTAC 1 cut(s) 30
DdeI CTNAG 3 cut(s) 107, 138, 344
DpnI GATC 2 cut(s) 216, 467
DpnII GATC 2 cut(s) 214, 465
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Eco24I GRGCYC 1 cut(s) 233
Eco31I GGTCTC 1 cut(s) 273
Eco32I GATATC 1 cut(s) 358
Eco81I CCTNAGG 1 cut(s) 344
EcoRV GATATC 1 cut(s) 358
EcoT38I GRGCYC 1 cut(s) 233
FaeI CATG 4 cut(s) 80, 326, 399, 436
FaiI YATR 4 cut(s) 78, 324, 397, 434
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 3 cut(s) 143, 298, 355
FatI CATG 4 cut(s) 76, 322, 395, 432
FbaI TGATCA 1 cut(s) 465
FokI GGATG 2 cut(s) 46, 221
FriOI GRGCYC 1 cut(s) 233
FspBI CTAG 2 cut(s) 351, 481
GsaI CCCAGC 1 cut(s) 285
HaeIII GGCC 1 cut(s) 407
HapII CCGG 2 cut(s) 126, 196
HgaI GACGC 1 cut(s) 170
Hin1II CATG 4 cut(s) 80, 326, 399, 436
HinfI GANTC 2 cut(s) 122, 319
HpaII CCGG 2 cut(s) 126, 196
HphI GGTGA 2 cut(s) 178, 211
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 2 cut(s) 97, 186
Hpy188III TCNNGA 1 cut(s) 361
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 2 cut(s) 101, 186
HpyAV CCTTC 1 cut(s) 407
HpyCH4V TGCA 1 cut(s) 59
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 44, 47, 50, 53, 56, 62
HpyF3I CTNAG 3 cut(s) 107, 138, 344
Hsp92II CATG 4 cut(s) 80, 326, 399, 436
Ksp22I TGATCA 1 cut(s) 465
Kzo9I GATC 2 cut(s) 214, 465
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 9 cut(s) 22, 25, 28, 31, 34, 37, 40, 43, 90
LweI GCATC 1 cut(s) 68
MaeI CTAG 2 cut(s) 351, 481
MaeIII GTNAC 1 cut(s) 79
MalI GATC 2 cut(s) 216, 467
MboI GATC 2 cut(s) 214, 465
MboII GAAGA 2 cut(s) 84, 256
MhlI GDGCHC 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 407
MluCI AATT 3 cut(s) 113, 312, 384
MluNI TGGCCA 1 cut(s) 407
MmeI TCCRAC 2 cut(s) 120, 164
MnlI CCTC 9 cut(s) 102, 127, 133, 144, 162, 255, 258, 353, 383
Mox20I TGGCCA 1 cut(s) 407
MscI TGGCCA 1 cut(s) 407
Msp20I TGGCCA 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 160
MspI CCGG 2 cut(s) 126, 196
MspR9I CCNGG 2 cut(s) 127, 197
MwoI GCNNNNNNNGC 7 cut(s) 41, 44, 47, 50, 53, 56, 62
NciI CCSGG 2 cut(s) 127, 197
NdeII GATC 2 cut(s) 214, 465
NlaIII CATG 4 cut(s) 80, 326, 399, 436
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 79
NspI RCATGY 1 cut(s) 80
PciI ACATGT 1 cut(s) 76
PfeI GAWTC 2 cut(s) 122, 319
PflFI GACNNNGTC 1 cut(s) 476
PscI ACATGT 1 cut(s) 76
PspFI CCCAGC 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 144
PsyI GACNNNGTC 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 160
RsaI GTAC 1 cut(s) 31
RsaNI GTAC 1 cut(s) 30
Sau3AI GATC 2 cut(s) 214, 465
ScrFI CCNGG 2 cut(s) 127, 197
SduI GDGCHC 1 cut(s) 233
SetI ASST 8 cut(s) 10, 105, 144, 162, 269, 345, 421, 429
SfaNI GCATC 1 cut(s) 68
Sse9I AATT 3 cut(s) 113, 312, 384
SsiI CCGC 4 cut(s) 63, 119, 164, 327
SspMI CTAG 2 cut(s) 351, 481
StyD4I CCNGG 2 cut(s) 125, 195
TaqI TCGA 1 cut(s) 176
TasI AATT 3 cut(s) 113, 312, 384
TatI WGTACW 1 cut(s) 29
TauI GCSGC 1 cut(s) 330
TfiI GAWTC 2 cut(s) 122, 319
TscAI CASTG 1 cut(s) 227
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 9 cut(s) 35, 38, 41, 44, 47, 50, 53, 56, 103
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 311, 421
TspRI CASTG 1 cut(s) 227
Tth111I GACNNNGTC 1 cut(s) 476
XapI RAATTY 1 cut(s) 113
XceI RCATGY 1 cut(s) 80
XspI CTAG 2 cut(s) 351, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.