MD12G1057100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014496
Physical Location & Seq
Reverse (-)
6531951 .. 6533864
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1057100.v1.1.491

Sequence Viewer

Length: 519 bp
ATGCTCTCTCTCTCTCTCTCTCTCTTTCTCTCTCTAATTTTACAATTTTACAAATTTACAATGGAATCTCTCACTACTGCAACTCCTACTACTTGTGCTAGTGTCCAACATGTGACCAAGAAGTCTTCAGACGAGCTGCTGAGGAAATTTGCCGATTCTGGGGACGAGGCCGATGCTCCGGCGAGCAAACGGATGGTGGTGAGGGTGTCGAAGCGCCGGAAAAGAAGCCGGGTGAACGGGGAAGGGGATCATCAGTGTGAGAGTCCGTCGAATGGGAAGACTACTGGATTGGTGGAGAGGAGGTCTCTGCTTCCTGCTGGGACTAAAAACAAGGCCTTGTTTAGGAAAATTGGGATTCATGGCGGCAGGGGTTCACACCTCAGGGCGAGGGATATCAGGAACAAGTCCCTCTTCCGTTCAATTCACAAGACATGGAGAAGGACCATTGAAGGAGCTTCAAAGGTTTTCATGGAAAAGCATTACAATAGGCACAAGCGTTTGATCAATGACATTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.49

Weight (kDa)

10.85

Isoelectric Point (pI)

60.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42110
fragaria_vesca FvH4_6g22320
malus_domestica MD12G1057100.v1.1 MD14G1057700.v1.1
prunus_persica Prupe.7G077000_v2.0.a1
pyrus_communis pycom12g05100 pycom14g04680
rosa_chinensis RchiOBHm_Chr3g0479681
rosa_laevigata RLG00000023548
rosa_multiflora Rmu_sc0003755.1_g000005
rosa_roxburghii Rroxscaffold_6G00402070
rosa_rugosa Rorug03G0177400
rosa_samantha Rh3AG228100 Rh3BG261300 Rh3CG258400 Rh3DG254600
rosa_wichuraiana Rw3G020410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 121
AciI CCGC 1 cut(s) 363
AclWI GGATC 1 cut(s) 255
AcsI RAATTY 2 cut(s) 53, 146
AcuI CTGAAG 1 cut(s) 111
AfiI CCNNNNNNNGG 3 cut(s) 159, 272, 342
AflIII ACRYGT 1 cut(s) 109
AgsI TTSAA 3 cut(s) 420, 449, 459
AluBI AGCT 2 cut(s) 136, 455
AluI AGCT 2 cut(s) 136, 455
Alw26I GTCTC 1 cut(s) 309
AlwI GGATC 1 cut(s) 255
AoxI GGCC 2 cut(s) 168, 333
ApeKI GCWGC 1 cut(s) 136
ApoI RAATTY 2 cut(s) 53, 146
AspLEI GCGC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 441
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 211, 244
AvaII GGWCC 1 cut(s) 441
AxyI CCTNAGG 1 cut(s) 380
BbsI GAAGAC 2 cut(s) 117, 284
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 1 cut(s) 123
BccI CCATC 1 cut(s) 187
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BclI TGATCA 1 cut(s) 501
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 309
BfaI CTAG 2 cut(s) 99, 517
BfoI RGCGCY 1 cut(s) 217
BisI GCNGC 2 cut(s) 137, 364
BlsI GCNGC 2 cut(s) 138, 365
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 1 cut(s) 441
BmgT120I GGNCC 1 cut(s) 441
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 163
BpiI GAAGAC 2 cut(s) 117, 284
BplI GAGNNNNNCTC 2 cut(s) 289, 321
Bpu10I CCTNAGC 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 230
BsaI GGTCTC 1 cut(s) 309
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 287, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 272, 342
Bse1I ACTGG 1 cut(s) 289
Bse21I CCTNAGG 1 cut(s) 380
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 3 cut(s) 159, 272, 342
BseMII CTCAG 2 cut(s) 131, 394
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 313
BseXI GCAGC 1 cut(s) 123
BseYI CCCAGC 1 cut(s) 317
BshFI GGCC 2 cut(s) 170, 335
BsiSI CCGG 3 cut(s) 179, 217, 229
BslFI GGGAC 3 cut(s) 176, 334, 391
BslI CCNNNNNNNGG 3 cut(s) 159, 272, 342
BsmAI GTCTC 1 cut(s) 309
BsmFI GGGAC 3 cut(s) 176, 334, 391
BsnI GGCC 2 cut(s) 170, 335
Bso31I GGTCTC 1 cut(s) 309
Bsp143I GATC 2 cut(s) 247, 501
BspACI CCGC 1 cut(s) 363
BspANI GGCC 2 cut(s) 170, 335
BspCNI CTCAG 2 cut(s) 132, 393
BspPI GGATC 1 cut(s) 255
BspTNI GGTCTC 1 cut(s) 309
BsrI ACTGG 1 cut(s) 289
BssMI GATC 2 cut(s) 247, 501
Bst6I CTCTTC 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 2 cut(s) 140, 380
BstENI CCTNNNNNAGG 1 cut(s) 340
BstF5I GGATG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 1 cut(s) 216
BstKTI GATC 2 cut(s) 250, 504
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 2 cut(s) 247, 501
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 123
BstV2I GAAGAC 2 cut(s) 117, 284
Bsu36I CCTNAGG 1 cut(s) 380
BsuRI GGCC 2 cut(s) 170, 335
BtsCI GGATG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 260
Cac8I GCNNGC 1 cut(s) 184
CfoI GCGC 1 cut(s) 216
Cfr13I GGNCC 1 cut(s) 441
CviAII CATG 4 cut(s) 110, 359, 432, 469
CviJI RGCY 5 cut(s) 136, 170, 228, 335, 455
CviKI_1 RGCY 5 cut(s) 136, 170, 228, 335, 455
DdeI CTNAG 2 cut(s) 140, 380
DpnI GATC 2 cut(s) 249, 503
DpnII GATC 2 cut(s) 247, 501
DrdI GACNNNNNNGTC 1 cut(s) 121
DseDI GACNNNNNNGTC 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
Eco147I AGGCCT 1 cut(s) 335
Eco31I GGTCTC 1 cut(s) 309
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 111
Eco81I CCTNAGG 1 cut(s) 380
EcoNI CCTNNNNNAGG 1 cut(s) 340
EcoRV GATATC 1 cut(s) 394
FaeI CATG 4 cut(s) 113, 362, 435, 472
FaiI YATR 4 cut(s) 111, 360, 433, 470
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 3 cut(s) 176, 334, 391
FatI CATG 4 cut(s) 109, 358, 431, 468
FbaI TGATCA 1 cut(s) 501
Fnu4HI GCNGC 2 cut(s) 137, 364
FokI GGATG 1 cut(s) 205
Fsp4HI GCNGC 2 cut(s) 137, 364
FspBI CTAG 2 cut(s) 99, 517
GlaI GCGC 1 cut(s) 215
GluI GCNGC 2 cut(s) 137, 364
GsaI CCCAGC 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 2 cut(s) 170, 335
HapII CCGG 3 cut(s) 179, 217, 229
HhaI GCGC 1 cut(s) 216
Hin1II CATG 4 cut(s) 113, 362, 435, 472
Hin6I GCGC 1 cut(s) 214
HinP1I GCGC 1 cut(s) 214
HinfI GANTC 4 cut(s) 65, 155, 262, 355
HpaII CCGG 3 cut(s) 179, 217, 229
HphI GGTGA 2 cut(s) 211, 244
Hpy166II GTNNAC 2 cut(s) 235, 374
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 1 cut(s) 397
Hpy8I GTNNAC 2 cut(s) 235, 374
Hpy99I CGWCG 1 cut(s) 271
HpyAV CCTTC 3 cut(s) 236, 432, 443
HpyCH4V TGCA 1 cut(s) 80
HpyF3I CTNAG 2 cut(s) 140, 380
Hsp92II CATG 4 cut(s) 113, 362, 435, 472
HspAI GCGC 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 501
Kzo9I GATC 2 cut(s) 247, 501
LmnI GCTCC 2 cut(s) 181, 452
Lsp1109I GCAGC 1 cut(s) 123
LweI GCATC 1 cut(s) 163
MaeI CTAG 2 cut(s) 99, 517
MaeIII GTNAC 1 cut(s) 112
MalI GATC 2 cut(s) 249, 503
MboI GATC 2 cut(s) 247, 501
MboII GAAGA 3 cut(s) 117, 289, 403
MluCI AATT 6 cut(s) 36, 44, 53, 146, 348, 420
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 8 cut(s) 135, 160, 195, 291, 294, 381, 389, 419
MslI CAYNNNNRTG 1 cut(s) 255
MspI CCGG 3 cut(s) 179, 217, 229
MspR9I CCNGG 1 cut(s) 230
NciI CCSGG 1 cut(s) 230
NdeII GATC 2 cut(s) 247, 501
NlaIII CATG 4 cut(s) 113, 362, 435, 472
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 1 cut(s) 113
PceI AGGCCT 1 cut(s) 335
PciI ACATGT 1 cut(s) 109
PfeI GAWTC 3 cut(s) 65, 155, 355
PflFI GACNNNGTC 1 cut(s) 512
PkrI GCNGC 2 cut(s) 138, 365
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PscI ACATGT 1 cut(s) 109
PspFI CCCAGC 1 cut(s) 317
PspPI GGNCC 1 cut(s) 441
PsyI GACNNNGTC 1 cut(s) 512
RseI CAYNNNNRTG 1 cut(s) 255
SatI GCNGC 2 cut(s) 137, 364
Sau3AI GATC 2 cut(s) 247, 501
Sau96I GGNCC 1 cut(s) 441
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 1 cut(s) 230
SetI ASST 5 cut(s) 138, 305, 381, 457, 465
SfaNI GCATC 1 cut(s) 163
SinI GGWCC 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 6 cut(s) 36, 44, 53, 146, 348, 420
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 1 cut(s) 363
SspMI CTAG 2 cut(s) 99, 517
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 1 cut(s) 228
TaqI TCGA 2 cut(s) 209, 269
TasI AATT 6 cut(s) 36, 44, 53, 146, 348, 420
TauI GCSGC 1 cut(s) 366
TfiI GAWTC 3 cut(s) 65, 155, 355
TscAI CASTG 1 cut(s) 260
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 2 cut(s) 347, 457
TspGWI ACGGA 3 cut(s) 205, 255, 404
TspRI CASTG 1 cut(s) 260
Tth111I GACNNNGTC 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 441
XagI CCTNNNNNAGG 1 cut(s) 340
XapI RAATTY 2 cut(s) 53, 146
XceI RCATGY 1 cut(s) 113
XspI CTAG 2 cut(s) 99, 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.