FvH4_6g22490

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001598
Physical Location & Seq
Reverse (-)
16160442 .. 16166816
6375 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22490.t13

Sequence Viewer

Length: 1005 bp
ATGGAGGCCCAAATTCATTACCTAGAGCAAGAAGCATACTGTGCAGTCCTGCGTGCTTTTAAAGCTCAGTCTGATGCGATTACTTGGGAAAAGGAAAGTTTGATTACAGAACTTAGGAAAGAACTGAGAGTATCAGATGATGAACACAGAGAGCTTCTTGGAAGGGTTAACTCTGATGATATCATCAGTAAGATAAGAGAGTGGAGAACGACAGGGGGGCCTCAAGCTGCGAGGTTCGGTGCATCACAACCTGTTTATGATCTTGTATCCAGTCCTACTGTTTCAGCATCCCGAAAGAAACAGAAAACATCCCAATCGCAGGGGCAGGCTTTCCCTGGTTTGTCCTCGATGAAGTCTATGCAGTACCATTCTACAGTACCTTCTTTGAGTCGGCAGCTCACTAGCCGCAGTTCTACTGGTCCCCTTGCAATATCTGAACCTGCTGAAGCATCAAGATATGATTTAATCGGAAAGACAGTGTGGATGAGATGGCCTGCAGACAACAGCTTCTATGAGGCCCATATAACAGATTACAACCCTAATGATGGTCGACATGCTTTGATCTATGATAAAAATACAGCAAATGAGACATTTGAATGGGTAGATTTCAAAGAGATATCTCGTGAGGATATCCGTTGGGAAGGTGAGGAACTGGGGCTCTCTCATCAAGGGAACCATGGCAGCGGACAAGGACGTAGGGTAAAGAAATCAACAAATCATGGTGGTCCAGTTACTGGGAGAGGTCGAGGACATATAAAGCCTCAATCAAAGAAACAACTTCTTCCTTCACAAAATGGCCTAGCAAACAAGTTGCCAGATGATCTAGAATTAGTGAATACTGATTCTCTGGTAAAGGAGGTCGAGAGAGTTTTCAATGCAAGTAATCCAGATCCCTTTGAGCTTGAAAAAGCAAGGAAAATGCTGAAAGATCACGAACAAGCACTTGTTGATGCCATTGAAAGGCTTGCATACGCATCGGATGGTGAAAGTGCTGAGCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

335

Amino Acids

37.54

Weight (kDa)

6.1

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 75 - 142 8.2e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 448
AccB7I CCANNNNNTGG 1 cut(s) 734
AccI GTMKAC 1 cut(s) 550
AciI CCGC 2 cut(s) 406, 684
AclWI GGATC 1 cut(s) 884
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 465
AfaI GTAC 2 cut(s) 365, 378
AfiI CCNNNNNNNGG 4 cut(s) 319, 545, 734, 960
AgsI TTSAA 5 cut(s) 596, 610, 874, 905, 959
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 6 cut(s) 65, 154, 227, 397, 507, 901
AluI AGCT 6 cut(s) 65, 154, 227, 397, 507, 901
Alw26I GTCTC 1 cut(s) 581
AlwI GGATC 1 cut(s) 884
AlwNI CAGNNNCTG 1 cut(s) 734
AoxI GGCC 5 cut(s) 6, 218, 491, 516, 796
ApeKI GCWGC 3 cut(s) 227, 394, 681
ApoI RAATTY 1 cut(s) 12
AspS9I GGNCC 5 cut(s) 7, 218, 419, 517, 725
AsuHPI GGTGA 2 cut(s) 656, 995
AvaII GGWCC 2 cut(s) 419, 725
BanII GRGCYC 1 cut(s) 660
BauI CACGAG 1 cut(s) 621
BbvI GCAGC 3 cut(s) 214, 406, 693
BccI CCATC 3 cut(s) 483, 539, 974
BcgI CGANNNNNNTGC 2 cut(s) 957, 991
BciT130I CCWGG 1 cut(s) 336
BciVI GTATCC 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 581
BfaI CTAG 4 cut(s) 23, 402, 800, 824
BfmI CTRYAG 2 cut(s) 372, 495
BfuAI ACCTGC 1 cut(s) 448
BfuI GTATCC 1 cut(s) 277
BisI GCNGC 4 cut(s) 228, 395, 406, 682
BlpI GCTNAGC 1 cut(s) 993
BlsI GCNGC 4 cut(s) 229, 396, 407, 683
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 2 cut(s) 419, 725
BmgT120I GGNCC 5 cut(s) 7, 218, 419, 517, 725
BmiI GGNNCC 3 cut(s) 219, 421, 674
BmrFI CCNGG 1 cut(s) 336
BmrI ACTGGG 2 cut(s) 662, 744
BmsI GCATC 6 cut(s) 64, 251, 296, 458, 940, 983
BmuI ACTGGG 2 cut(s) 662, 744
Bpu1102I GCTNAGC 1 cut(s) 993
BpuEI CTTGAG 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 334, 676
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 545, 734, 960
Bse1I ACTGG 5 cut(s) 270, 421, 657, 728, 739
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 334, 676
BseGI GGATG 4 cut(s) 287, 308, 489, 985
BseLI CCNNNNNNNGG 4 cut(s) 319, 545, 734, 960
BseMII CTCAG 3 cut(s) 80, 116, 984
BseNI ACTGG 5 cut(s) 270, 421, 657, 728, 739
BseXI GCAGC 3 cut(s) 214, 406, 693
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 5 cut(s) 8, 220, 493, 518, 798
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 4 cut(s) 319, 545, 734, 960
BsmAI GTCTC 1 cut(s) 581
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 5 cut(s) 8, 220, 493, 518, 798
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 5 cut(s) 259, 561, 820, 889, 928
Bsp1720I GCTNAGC 1 cut(s) 993
Bsp19I CCATGG 1 cut(s) 676
BspACI CCGC 2 cut(s) 406, 684
BspANI GGCC 5 cut(s) 8, 220, 493, 518, 798
BspCNI CTCAG 3 cut(s) 79, 117, 985
BspLI GGNNCC 3 cut(s) 219, 421, 674
BspMAI CTGCAG 1 cut(s) 499
BspMI ACCTGC 1 cut(s) 448
BspPI GGATC 1 cut(s) 884
BsrI ACTGG 5 cut(s) 270, 421, 657, 728, 739
BssECI CCNNGG 2 cut(s) 334, 676
BssMI GATC 5 cut(s) 259, 561, 820, 889, 928
BssSI CACGAG 1 cut(s) 621
BssT1I CCWWGG 1 cut(s) 676
Bst2BI CACGAG 1 cut(s) 621
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 4 cut(s) 41, 280, 376, 478
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 54, 327, 495, 966
BstDEI CTNAG 4 cut(s) 66, 113, 125, 993
BstDSI CCRYGG 1 cut(s) 676
BstF5I GGATG 4 cut(s) 287, 308, 489, 985
BstKTI GATC 5 cut(s) 262, 564, 823, 892, 931
BstMAI GTCTC 1 cut(s) 581
BstMBI GATC 5 cut(s) 259, 561, 820, 889, 928
BstMWI GCNNNNNNNGC 2 cut(s) 41, 62
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 1 cut(s) 557
BstSCI CCNGG 1 cut(s) 334
BstSFI CTRYAG 2 cut(s) 372, 495
BstV1I GCAGC 3 cut(s) 214, 406, 693
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
BsuI GTATCC 1 cut(s) 277
BsuRI GGCC 5 cut(s) 8, 220, 493, 518, 798
BtgI CCRYGG 1 cut(s) 676
BtsCI GGATG 4 cut(s) 287, 308, 489, 985
BtsIMutI CAGTG 1 cut(s) 483
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 4 cut(s) 54, 327, 495, 966
CaiI CAGNNNCTG 1 cut(s) 734
Cfr13I GGNCC 5 cut(s) 7, 218, 419, 517, 725
Csp6I GTAC 2 cut(s) 364, 377
CviAII CATG 3 cut(s) 554, 677, 719
CviQI GTAC 2 cut(s) 364, 377
DdeI CTNAG 4 cut(s) 66, 113, 125, 993
DpnI GATC 5 cut(s) 261, 563, 822, 891, 930
DpnII GATC 5 cut(s) 259, 561, 820, 889, 928
DraI TTTAAA 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 676
Eco24I GRGCYC 1 cut(s) 660
Eco32I GATATC 3 cut(s) 181, 618, 631
Eco47I GGWCC 2 cut(s) 419, 725
Eco57I CTGAAG 1 cut(s) 465
EcoO109I RGGNCCY 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 334
EcoRV GATATC 3 cut(s) 181, 618, 631
EcoT14I CCWWGG 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 660
ErhI CCWWGG 1 cut(s) 676
FaeI CATG 3 cut(s) 557, 680, 722
FaqI GGGAC 1 cut(s) 405
FatI CATG 3 cut(s) 553, 676, 718
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 4 cut(s) 228, 395, 406, 682
FokI GGATG 4 cut(s) 274, 295, 496, 992
FriOI GRGCYC 1 cut(s) 660
Fsp4HI GCNGC 4 cut(s) 228, 395, 406, 682
FspBI CTAG 4 cut(s) 23, 402, 800, 824
GluI GCNGC 4 cut(s) 228, 395, 406, 682
HaeIII GGCC 5 cut(s) 8, 220, 493, 518, 798
Hin1II CATG 3 cut(s) 557, 680, 722
HincII GTYRAC 2 cut(s) 169, 551
HindII GTYRAC 2 cut(s) 169, 551
HinfI GANTC 2 cut(s) 388, 842
HpaI GTTAAC 1 cut(s) 169
HphI GGTGA 2 cut(s) 656, 995
Hpy166II GTNNAC 2 cut(s) 169, 551
Hpy188I TCNGA 6 cut(s) 73, 136, 175, 436, 470, 979
Hpy188III TCNNGA 7 cut(s) 291, 453, 623, 824, 862, 887, 932
Hpy8I GTNNAC 2 cut(s) 169, 551
HpyAV CCTTC 4 cut(s) 156, 390, 635, 795
HpyCH4III ACNGT 4 cut(s) 41, 280, 376, 478
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 7 cut(s) 44, 242, 361, 428, 497, 878, 968
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 62
HpyF3I CTNAG 4 cut(s) 66, 113, 125, 993
HpySE526I ACGT 1 cut(s) 694
Hsp92II CATG 3 cut(s) 557, 680, 722
KspAI GTTAAC 1 cut(s) 169
Kzo9I GATC 5 cut(s) 259, 561, 820, 889, 928
Lsp1109I GCAGC 3 cut(s) 214, 406, 693
LweI GCATC 6 cut(s) 64, 251, 296, 458, 940, 983
MaeI CTAG 4 cut(s) 23, 402, 800, 824
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 1 cut(s) 730
MalI GATC 5 cut(s) 261, 563, 822, 891, 930
MboI GATC 5 cut(s) 259, 561, 820, 889, 928
MboII GAAGA 1 cut(s) 773
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 660
MluCI AATT 2 cut(s) 12, 827
MlyI GAGTC 1 cut(s) 397
MseI TTAA 3 cut(s) 60, 168, 464
MslI CAYNNNNRTG 1 cut(s) 595
MspA1I CMGCKG 1 cut(s) 684
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 2 cut(s) 41, 62
NcoI CCATGG 1 cut(s) 676
NdeII GATC 5 cut(s) 259, 561, 820, 889, 928
NlaIII CATG 3 cut(s) 557, 680, 722
NlaIV GGNNCC 3 cut(s) 219, 421, 674
NspI RCATGY 1 cut(s) 557
PfeI GAWTC 1 cut(s) 842
PflMI CCANNNNNTGG 1 cut(s) 734
PkrI GCNGC 4 cut(s) 229, 396, 407, 683
PleI GAGTC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 3 cut(s) 219, 421, 674
PspPI GGNCC 5 cut(s) 7, 218, 419, 517, 725
PstI CTGCAG 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 2 cut(s) 365, 378
RsaNI GTAC 2 cut(s) 364, 377
RseI CAYNNNNRTG 1 cut(s) 595
SalI GTCGAC 1 cut(s) 549
SaqAI TTAA 3 cut(s) 60, 168, 464
SatI GCNGC 4 cut(s) 228, 395, 406, 682
Sau3AI GATC 5 cut(s) 259, 561, 820, 889, 928
Sau96I GGNCC 5 cut(s) 7, 218, 419, 517, 725
SchI GAGTC 1 cut(s) 397
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 660
SfaNI GCATC 6 cut(s) 64, 251, 296, 458, 940, 983
SfcI CTRYAG 2 cut(s) 372, 495
SinI GGWCC 2 cut(s) 419, 725
SmiMI CAYNNNNRTG 1 cut(s) 595
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
Sse9I AATT 2 cut(s) 12, 827
SsiI CCGC 2 cut(s) 406, 684
SspMI CTAG 4 cut(s) 23, 402, 800, 824
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 4 cut(s) 41, 280, 376, 478
TaiI ACGT 1 cut(s) 697
TaqI TCGA 4 cut(s) 347, 550, 745, 861
TasI AATT 2 cut(s) 12, 827
TauI GCSGC 1 cut(s) 408
TfiI GAWTC 1 cut(s) 842
Tru1I TTAA 3 cut(s) 60, 168, 464
Tru9I TTAA 3 cut(s) 60, 168, 464
TscAI CASTG 1 cut(s) 483
TseI GCWGC 3 cut(s) 227, 394, 681
TspDTI ATGAA 3 cut(s) 5, 156, 365
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 1 cut(s) 483
Van91I CCANNNNNTGG 1 cut(s) 734
VpaK11BI GGWCC 2 cut(s) 419, 725
XapI RAATTY 1 cut(s) 12
XbaI TCTAGA 1 cut(s) 823
XceI RCATGY 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 550
XspI CTAG 4 cut(s) 23, 402, 800, 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.