RLG00000023526

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001598
Physical Location & Seq
Forward (+)
24931244 .. 24936764
5521 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023526

Sequence Viewer

Length: 1020 bp
ATGGAGGCCCAAATTCATCACCTAGAGCAAGAAGCATACTGTGCAGTCCTGCGTGCTTTCAAAGCTCAGTCTGATGCGATTACTTGGGAGAAGGAAGGTTTGATTACAGAACTTAGGAAAGAACTGAGAGTATCCGATGATGAACACAGAGAGCTTCTTGGCAGGGTTAACTCTGATGATATCATCAGTAAGATAAGAGAGTGGAGAACGGCAGGTGGGCATCAAGCTGCGAGATTCAGTGCATCCCAGCCTGTTTATGACCTTGTATCCAGTCCTACTGTTTCAGCATCCCGAAAGAAACAGAAAACATCCCAATCGCAGGGTCAGCCATTCCCTGTTTTATCCTCAATGAAGTCTATGCAGTACCCTTCTACAGGACCTTCGTTGAGTAGGCAGCTCACTAGCCGCAGTACTACTGGTCCCCTTGCCATAACTGAACCTGCTGAAGCAGCAACATATGATTTAATTGGAAAAAAAGTGTGGATGAGATGGCCTGCAGACAACAGCTTCTATGAGGCCCTTATAACAGACTACAACCCTAATGATGGTCGACATGCTTTGATCTACGATAAAAATACAGCAAATGAGACATTTGAATGGGTAGATTTCAAAGAGATATCTCGTGAGGATATCCGTTGGGAAGGTGAGGAACTGGGGCTATCTCATCAAGGGAACCATGGCAGTGGACAAGGACGTAGGGTTAAGAAATCTACGAACCATGGTGGTGTTGGTCCAGTTTCTGGGAGAGGTCGAGGACATATAAAGCCTCAATCAAAGAAACAACTTCTTCCTTCACAAAATGGCATAGGAAATAAGTTGCCAGATGATCTAGAATTATTGAATACTGATTCTCTGGTAAAGGAGGTGGAGAGAGTTTTCAATGCAAGTAATCCGGACCCCTTTGAGCTTGAAAAGGCAAGGAAAATGCTGAAAGATCACGAACAGGCACTTGTTGATGCCATTGAAAGGCTTGCATATGCATCGGATGGTGAAAGTGGTAATGGTTATATGTGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

340

Amino Acids

37.87

Weight (kDa)

6.24

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 3 - 71 3.7e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AarI CACCTGC 1 cut(s) 203
Acc36I ACCTGC 2 cut(s) 203, 448
AccB7I CCANNNNNTGG 1 cut(s) 740
AccI GTMKAC 1 cut(s) 550
AccIII TCCGGA 1 cut(s) 892
AciI CCGC 1 cut(s) 406
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 465
AfaI GTAC 3 cut(s) 365, 412, 1015
AfiI CCNNNNNNNGG 5 cut(s) 319, 374, 545, 740, 966
AgsI TTSAA 7 cut(s) 61, 596, 610, 841, 880, 911, 965
AluBI AGCT 6 cut(s) 65, 154, 227, 397, 507, 907
AluI AGCT 6 cut(s) 65, 154, 227, 397, 507, 907
Alw26I GTCTC 1 cut(s) 581
AlwNI CAGNNNCTG 1 cut(s) 740
Aor13HI TCCGGA 1 cut(s) 892
AoxI GGCC 3 cut(s) 6, 491, 516
ApeKI GCWGC 3 cut(s) 227, 394, 449
ApoI RAATTY 1 cut(s) 12
AspS9I GGNCC 6 cut(s) 7, 377, 419, 517, 731, 895
AsuHPI GGTGA 3 cut(s) 11, 656, 1001
AvaII GGWCC 4 cut(s) 377, 419, 731, 895
BauI CACGAG 1 cut(s) 621
BbvI GCAGC 3 cut(s) 214, 406, 461
BccI CCATC 3 cut(s) 483, 539, 980
BceAI ACGGC 1 cut(s) 225
BcgI CGANNNNNNTGC 2 cut(s) 963, 997
BciVI GTATCC 2 cut(s) 142, 277
BcoDI GTCTC 1 cut(s) 581
BfaI CTAG 3 cut(s) 23, 402, 830
BfmI CTRYAG 2 cut(s) 372, 495
BfuAI ACCTGC 2 cut(s) 203, 448
BfuI GTATCC 2 cut(s) 142, 277
BisI GCNGC 4 cut(s) 228, 395, 406, 450
BlsI GCNGC 4 cut(s) 229, 396, 407, 451
BmcAI AGTACT 1 cut(s) 412
Bme18I GGWCC 4 cut(s) 377, 419, 731, 895
BmgT120I GGNCC 6 cut(s) 7, 377, 419, 517, 731, 895
BmiI GGNNCC 3 cut(s) 421, 674, 897
BmrI ACTGGG 1 cut(s) 662
BmsI GCATC 6 cut(s) 64, 229, 251, 296, 946, 989
BmuI ACTGGG 1 cut(s) 662
BsaJI CCNNGG 2 cut(s) 676, 718
BsaWI WCCGGW 1 cut(s) 892
Bsc4I CCNNNNNNNGG 5 cut(s) 319, 374, 545, 740, 966
Bse1I ACTGG 4 cut(s) 270, 421, 657, 734
BseAI TCCGGA 1 cut(s) 892
BseDI CCNNGG 2 cut(s) 676, 718
BseGI GGATG 5 cut(s) 242, 287, 308, 489, 991
BseLI CCNNNNNNNGG 5 cut(s) 319, 374, 545, 740, 966
BseMII CTCAG 2 cut(s) 80, 116
BseNI ACTGG 4 cut(s) 270, 421, 657, 734
BseXI GCAGC 3 cut(s) 214, 406, 461
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 3 cut(s) 8, 493, 518
BsiSI CCGG 1 cut(s) 893
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 5 cut(s) 319, 374, 545, 740, 966
BsmAI GTCTC 1 cut(s) 581
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 3 cut(s) 8, 493, 518
Bsp13I TCCGGA 1 cut(s) 892
Bsp1407I TGTACA 1 cut(s) 1013
Bsp143I GATC 3 cut(s) 561, 826, 934
Bsp19I CCATGG 2 cut(s) 676, 718
BspACI CCGC 1 cut(s) 406
BspANI GGCC 3 cut(s) 8, 493, 518
BspCNI CTCAG 2 cut(s) 79, 117
BspEI TCCGGA 1 cut(s) 892
BspLI GGNNCC 3 cut(s) 421, 674, 897
BspMAI CTGCAG 1 cut(s) 499
BspMI ACCTGC 2 cut(s) 203, 448
BsrGI TGTACA 1 cut(s) 1013
BsrI ACTGG 4 cut(s) 270, 421, 657, 734
BssECI CCNNGG 2 cut(s) 676, 718
BssMI GATC 3 cut(s) 561, 826, 934
BssSI CACGAG 1 cut(s) 621
BssT1I CCWWGG 2 cut(s) 676, 718
Bst2BI CACGAG 1 cut(s) 621
Bst4CI ACNGT 2 cut(s) 41, 280
BstAPI GCANNNNNTGC 1 cut(s) 41
BstAUI TGTACA 1 cut(s) 1013
BstC8I GCNNGC 3 cut(s) 54, 495, 972
BstDEI CTNAG 3 cut(s) 66, 113, 125
BstDSI CCRYGG 2 cut(s) 676, 718
BstENI CCTNNNNNAGG 1 cut(s) 372
BstF5I GGATG 5 cut(s) 242, 287, 308, 489, 991
BstKTI GATC 3 cut(s) 564, 829, 937
BstMAI GTCTC 1 cut(s) 581
BstMBI GATC 3 cut(s) 561, 826, 934
BstMWI GCNNNNNNNGC 4 cut(s) 41, 62, 325, 449
BstNSI RCATGY 1 cut(s) 557
BstSFI CTRYAG 2 cut(s) 372, 495
BstV1I GCAGC 3 cut(s) 214, 406, 461
BstXI CCANNNNNNTGG 1 cut(s) 683
BsuI GTATCC 2 cut(s) 142, 277
BsuRI GGCC 3 cut(s) 8, 493, 518
BtgI CCRYGG 2 cut(s) 676, 718
BtsCI GGATG 5 cut(s) 242, 287, 308, 489, 991
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 2 cut(s) 244, 688
BveI ACCTGC 2 cut(s) 203, 448
Cac8I GCNNGC 3 cut(s) 54, 495, 972
CaiI CAGNNNCTG 1 cut(s) 740
Cfr13I GGNCC 6 cut(s) 7, 377, 419, 517, 731, 895
Csp6I GTAC 3 cut(s) 364, 411, 1014
CviAII CATG 3 cut(s) 554, 677, 719
CviQI GTAC 3 cut(s) 364, 411, 1014
DdeI CTNAG 3 cut(s) 66, 113, 125
DpnI GATC 3 cut(s) 563, 828, 936
DpnII GATC 3 cut(s) 561, 826, 934
Eco130I CCWWGG 2 cut(s) 676, 718
Eco32I GATATC 3 cut(s) 181, 618, 631
Eco47I GGWCC 4 cut(s) 377, 419, 731, 895
Eco57I CTGAAG 1 cut(s) 465
EcoNI CCTNNNNNAGG 1 cut(s) 372
EcoO109I RGGNCCY 2 cut(s) 377, 517
EcoRV GATATC 3 cut(s) 181, 618, 631
EcoT14I CCWWGG 2 cut(s) 676, 718
EcoT22I ATGCAT 1 cut(s) 982
ErhI CCWWGG 2 cut(s) 676, 718
FaeI CATG 3 cut(s) 557, 680, 722
FaqI GGGAC 1 cut(s) 405
FatI CATG 3 cut(s) 553, 676, 718
FauNDI CATATG 2 cut(s) 457, 976
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 4 cut(s) 228, 395, 406, 450
FokI GGATG 5 cut(s) 229, 274, 295, 496, 998
Fsp4HI GCNGC 4 cut(s) 228, 395, 406, 450
FspBI CTAG 3 cut(s) 23, 402, 830
GluI GCNGC 4 cut(s) 228, 395, 406, 450
GsaI CCCAGC 1 cut(s) 250
HaeIII GGCC 3 cut(s) 8, 493, 518
HapII CCGG 1 cut(s) 893
Hin1II CATG 3 cut(s) 557, 680, 722
HincII GTYRAC 2 cut(s) 169, 551
HindII GTYRAC 2 cut(s) 169, 551
HinfI GANTC 2 cut(s) 234, 848
HpaI GTTAAC 1 cut(s) 169
HpaII CCGG 1 cut(s) 893
HphI GGTGA 3 cut(s) 11, 656, 1001
Hpy166II GTNNAC 4 cut(s) 169, 551, 686, 1014
Hpy188I TCNGA 4 cut(s) 73, 136, 175, 985
Hpy188III TCNNGA 5 cut(s) 291, 623, 830, 893, 938
Hpy8I GTNNAC 4 cut(s) 169, 551, 686, 1014
HpyAV CCTTC 6 cut(s) 85, 89, 378, 390, 635, 801
HpyCH4III ACNGT 2 cut(s) 41, 280
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 7 cut(s) 44, 242, 361, 497, 884, 974, 980
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 62, 325, 449
HpyF3I CTNAG 3 cut(s) 66, 113, 125
HpySE526I ACGT 1 cut(s) 694
Hsp92II CATG 3 cut(s) 557, 680, 722
Kpn2I TCCGGA 1 cut(s) 892
KspAI GTTAAC 1 cut(s) 169
Kzo9I GATC 3 cut(s) 561, 826, 934
Lsp1109I GCAGC 3 cut(s) 214, 406, 461
LweI GCATC 6 cut(s) 64, 229, 251, 296, 946, 989
MaeI CTAG 3 cut(s) 23, 402, 830
MaeII ACGT 1 cut(s) 694
MalI GATC 3 cut(s) 563, 828, 936
MboI GATC 3 cut(s) 561, 826, 934
MboII GAAGA 1 cut(s) 779
MluCI AATT 3 cut(s) 12, 465, 833
MnlI CCTC 8 cut(s) 355, 508, 619, 640, 740, 746, 777, 856
Mph1103I ATGCAT 1 cut(s) 982
MroI TCCGGA 1 cut(s) 892
MseI TTAA 3 cut(s) 168, 464, 702
MslI CAYNNNNRTG 3 cut(s) 595, 681, 723
MspI CCGG 1 cut(s) 893
MwoI GCNNNNNNNGC 4 cut(s) 41, 62, 325, 449
NcoI CCATGG 2 cut(s) 676, 718
NdeI CATATG 2 cut(s) 457, 976
NdeII GATC 3 cut(s) 561, 826, 934
NlaIII CATG 3 cut(s) 557, 680, 722
NlaIV GGNNCC 3 cut(s) 421, 674, 897
NsiI ATGCAT 1 cut(s) 982
NspI RCATGY 1 cut(s) 557
PaqCI CACCTGC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 234, 848
PflMI CCANNNNNTGG 1 cut(s) 740
PkrI GCNGC 4 cut(s) 229, 396, 407, 451
PpuMI RGGWCCY 1 cut(s) 377
PsiI TTATAA 1 cut(s) 524
Psp5II RGGWCCY 1 cut(s) 377
PspFI CCCAGC 1 cut(s) 246
PspN4I GGNNCC 3 cut(s) 421, 674, 897
PspPI GGNCC 6 cut(s) 7, 377, 419, 517, 731, 895
PspPPI RGGWCCY 1 cut(s) 377
PstI CTGCAG 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 740
RsaI GTAC 3 cut(s) 365, 412, 1015
RsaNI GTAC 3 cut(s) 364, 411, 1014
RseI CAYNNNNRTG 3 cut(s) 595, 681, 723
SalI GTCGAC 1 cut(s) 549
SaqAI TTAA 3 cut(s) 168, 464, 702
SatI GCNGC 4 cut(s) 228, 395, 406, 450
Sau3AI GATC 3 cut(s) 561, 826, 934
Sau96I GGNCC 6 cut(s) 7, 377, 419, 517, 731, 895
ScaI AGTACT 1 cut(s) 412
SfaNI GCATC 6 cut(s) 64, 229, 251, 296, 946, 989
SfcI CTRYAG 2 cut(s) 372, 495
SinI GGWCC 4 cut(s) 377, 419, 731, 895
SmiMI CAYNNNNRTG 3 cut(s) 595, 681, 723
Sse9I AATT 3 cut(s) 12, 465, 833
SsiI CCGC 1 cut(s) 406
SspMI CTAG 3 cut(s) 23, 402, 830
StyI CCWWGG 2 cut(s) 676, 718
TaaI ACNGT 2 cut(s) 41, 280
TaiI ACGT 1 cut(s) 697
TaqI TCGA 2 cut(s) 550, 751
TasI AATT 3 cut(s) 12, 465, 833
TatI WGTACW 2 cut(s) 410, 1013
TauI GCSGC 1 cut(s) 408
TfiI GAWTC 2 cut(s) 234, 848
Tru1I TTAA 3 cut(s) 168, 464, 702
Tru9I TTAA 3 cut(s) 168, 464, 702
TscAI CASTG 2 cut(s) 244, 688
TseI GCWGC 3 cut(s) 227, 394, 449
TspDTI ATGAA 3 cut(s) 5, 156, 365
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 2 cut(s) 244, 688
Van91I CCANNNNNTGG 1 cut(s) 740
VpaK11BI GGWCC 4 cut(s) 377, 419, 731, 895
XagI CCTNNNNNAGG 1 cut(s) 372
XapI RAATTY 1 cut(s) 12
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 725
XmiI GTMKAC 1 cut(s) 550
XspI CTAG 3 cut(s) 23, 402, 830
ZrmI AGTACT 1 cut(s) 412
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.