Rorug03G0179400

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001598
Physical Location & Seq
Reverse (-)
15347781 .. 15348542
762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0179400.1

Sequence Viewer

Length: 762 bp
ATGCCAGCGTGCGTTTCTTTTTATCGCAATCGTCCTCCTCCCTACGAATTCAAAGACAACAAAGACCGTGAGAAGGACATACTTCTCATTAAATTCCTTATGGGTATACCCTGCGTCTTGTTATTAAGCTTCGCCGTGTACCAAAAGTTCTTGGAATACAAGATGAATCTTCAAAGACCGCAGTTTCAGATCGACTCCGCTTCGGTCTCCGGAGTGAGCGTCACCGGCTGCCAGTTAACTGCCACGTGGAACATCACCCTCCTCGCCGCAAACCCTAACCTGCACCTCGGCGTTGACTATCTGACCATCGACTCGTCCCTCTATCATGGAGACAAGAAAGACGTGCTCTACTGGGATGGGAGATCCAAGACGACATGCATTATCTCAATCGCAACCACGAGGTTGTGTAAGCCATTCTTCCTAGACAAAGGAGGGAACCGGACCACCCCTGTCAATTTCAAACTCGCAGTAGTGAATAAGGACGTCTGCTGTGGCTTGGCGAAGGAGATCTCAGAGGTAGCGGCTCGTGGTGGCAAAGTCAAATTCGGGCTGCGCATCAGGCTTTTCTTTTATTACAGAACTAAGAATTGGGGCCTGCGAGTTCTCCATGATGTTAGCTTCTTCTGTAATCGGGTCGAATTCGTGTTCCCTCCGAATAATAATCATGGCACTGGAATTTTCAAAGGTCACTCGCGGAAATGCCCAGATTCTTGCTCAACTCCCAATTGTATGCATAGTTGGTCCGTGACATCAAGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

253

Amino Acids

28.63

Weight (kDa)

9.37

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 486
Acc16I TGCGCA 1 cut(s) 554
Acc36I ACCTGC 1 cut(s) 288
AccI GTMKAC 1 cut(s) 106
AccII CGCG 1 cut(s) 694
AccIII TCCGGA 1 cut(s) 209
AciI CCGC 5 cut(s) 179, 198, 267, 521, 694
AclWI GGATC 1 cut(s) 357
AcsI RAATTY 5 cut(s) 47, 92, 542, 638, 675
AcvI CACGTG 1 cut(s) 246
AcyI GRCGYC 1 cut(s) 483
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 4 cut(s) 52, 173, 460, 682
AjiI CACGTC 1 cut(s) 343
AjuI GAANNNNNNNTTGG 2 cut(s) 571, 603
AluBI AGCT 2 cut(s) 129, 618
AluI AGCT 2 cut(s) 129, 618
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 2 cut(s) 211, 324
AlwI GGATC 1 cut(s) 357
Aor13HI TCCGGA 1 cut(s) 209
AoxI GGCC 1 cut(s) 592
ApeKI GCWGC 2 cut(s) 228, 550
ApoI RAATTY 5 cut(s) 47, 92, 542, 638, 675
AspLEI GCGC 1 cut(s) 555
AspS9I GGNCC 3 cut(s) 441, 592, 741
AsuHPI GGTGA 2 cut(s) 214, 247
AvaII GGWCC 2 cut(s) 441, 741
BauI CACGAG 2 cut(s) 397, 525
BbrPI CACGTG 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 215, 537
BccI CCATC 2 cut(s) 314, 350
BceAI ACGGC 1 cut(s) 119
BcoDI GTCTC 2 cut(s) 211, 324
BfaI CTAG 1 cut(s) 422
BfuAI ACCTGC 1 cut(s) 288
BglII AGATCT 1 cut(s) 507
BisI GCNGC 4 cut(s) 229, 267, 522, 551
BlsI GCNGC 4 cut(s) 230, 268, 523, 552
Bme18I GGWCC 2 cut(s) 441, 741
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 3 cut(s) 441, 592, 741
BmiI GGNNCC 2 cut(s) 437, 593
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 1 cut(s) 564
BmuI ACTGGG 1 cut(s) 361
BsaAI YACGTR 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 211
BsaJI CCNNGG 1 cut(s) 286
BsaWI WCCGGW 2 cut(s) 209, 438
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 3 cut(s) 232, 356, 676
BseAI TCCGGA 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 525
BseNI ACTGG 3 cut(s) 232, 356, 676
BseRI GAGGAG 2 cut(s) 27, 251
BseXI GCAGC 2 cut(s) 215, 537
BsgI GTGCAG 1 cut(s) 266
Bsh1236I CGCG 1 cut(s) 694
BshFI GGCC 1 cut(s) 594
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 3 cut(s) 210, 225, 439
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 2 cut(s) 211, 324
BsmFI GGGAC 1 cut(s) 301
BsnI GGCC 1 cut(s) 594
Bso31I GGTCTC 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 348
Bsp13I TCCGGA 1 cut(s) 209
Bsp143I GATC 3 cut(s) 189, 362, 507
BspACI CCGC 5 cut(s) 179, 198, 267, 521, 694
BspANI GGCC 1 cut(s) 594
BspCNI CTCAG 1 cut(s) 524
BspEI TCCGGA 1 cut(s) 209
BspFNI CGCG 1 cut(s) 694
BspLI GGNNCC 2 cut(s) 437, 593
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 1 cut(s) 357
BspTNI GGTCTC 1 cut(s) 211
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 3 cut(s) 232, 356, 676
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 3 cut(s) 189, 362, 507
BssNAI GTATAC 1 cut(s) 107
BssNI GRCGYC 1 cut(s) 483
BssSI CACGAG 2 cut(s) 397, 525
Bst1107I GTATAC 1 cut(s) 107
Bst2BI CACGAG 2 cut(s) 397, 525
Bst4CI ACNGT 1 cut(s) 68
BstACI GRCGYC 1 cut(s) 483
BstBAI YACGTR 1 cut(s) 246
BstC8I GCNNGC 3 cut(s) 6, 10, 596
BstDEI CTNAG 2 cut(s) 511, 582
BstF5I GGATG 1 cut(s) 361
BstFNI CGCG 1 cut(s) 694
BstHHI GCGC 1 cut(s) 555
BstKTI GATC 3 cut(s) 192, 365, 510
BstMAI GTCTC 2 cut(s) 211, 324
BstMBI GATC 3 cut(s) 189, 362, 507
BstMWI GCNNNNNNNGC 2 cut(s) 225, 559
BstNSI RCATGY 1 cut(s) 378
BstUI CGCG 1 cut(s) 694
BstV1I GCAGC 2 cut(s) 215, 537
BstX2I RGATCY 2 cut(s) 362, 507
BstYI RGATCY 2 cut(s) 362, 507
BstZ17I GTATAC 1 cut(s) 107
BsuRI GGCC 1 cut(s) 594
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 669
BveI ACCTGC 1 cut(s) 288
Cac8I GCNNGC 3 cut(s) 6, 10, 596
CfoI GCGC 1 cut(s) 555
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 3 cut(s) 441, 592, 741
CseI GACGC 2 cut(s) 103, 208
Csp6I GTAC 1 cut(s) 139
CviAII CATG 4 cut(s) 326, 375, 608, 665
CviJI RGCY 9 cut(s) 129, 228, 412, 495, 524, 550, 562, 594, 618
CviKI_1 RGCY 9 cut(s) 129, 228, 412, 495, 524, 550, 562, 594, 618
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 2 cut(s) 511, 582
DpnI GATC 3 cut(s) 191, 364, 509
DpnII GATC 3 cut(s) 189, 362, 507
Eco31I GGTCTC 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 441, 741
Eco72I CACGTG 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 592
EcoRI GAATTC 2 cut(s) 47, 638
EcoT22I ATGCAT 2 cut(s) 380, 735
FaeI CATG 4 cut(s) 329, 378, 611, 668
FaiI YATR 9 cut(s) 80, 101, 107, 327, 376, 609, 666, 731, 735
FalI AAGNNNNNCTT 2 cut(s) 401, 433
FaqI GGGAC 1 cut(s) 301
FatI CATG 4 cut(s) 325, 374, 607, 664
FblI GTMKAC 1 cut(s) 106
Fnu4HI GCNGC 4 cut(s) 229, 267, 522, 551
FokI GGATG 1 cut(s) 368
Fsp4HI GCNGC 4 cut(s) 229, 267, 522, 551
FspBI CTAG 1 cut(s) 422
FspI TGCGCA 1 cut(s) 554
GlaI GCGC 1 cut(s) 554
GluI GCNGC 4 cut(s) 229, 267, 522, 551
HaeIII GGCC 1 cut(s) 594
HapII CCGG 3 cut(s) 210, 225, 439
HgaI GACGC 2 cut(s) 103, 208
HhaI GCGC 1 cut(s) 555
Hin1I GRCGYC 1 cut(s) 483
Hin1II CATG 4 cut(s) 329, 378, 611, 668
Hin6I GCGC 1 cut(s) 553
HinP1I GCGC 1 cut(s) 553
HincII GTYRAC 2 cut(s) 237, 295
HindII GTYRAC 2 cut(s) 237, 295
HindIII AAGCTT 1 cut(s) 127
HinfI GANTC 4 cut(s) 166, 194, 311, 707
HpaI GTTAAC 1 cut(s) 237
HpaII CCGG 3 cut(s) 210, 225, 439
HphI GGTGA 2 cut(s) 214, 247
Hpy166II GTNNAC 4 cut(s) 107, 139, 237, 295
Hpy188I TCNGA 4 cut(s) 189, 303, 514, 654
Hpy188III TCNNGA 1 cut(s) 210
Hpy8I GTNNAC 4 cut(s) 107, 139, 237, 295
HpyAV CCTTC 2 cut(s) 67, 496
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4IV ACGT 3 cut(s) 245, 342, 483
HpyCH4V TGCA 3 cut(s) 283, 378, 733
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 559
HpyF3I CTNAG 2 cut(s) 511, 582
HpySE526I ACGT 3 cut(s) 245, 342, 483
Hsp92I GRCGYC 1 cut(s) 483
Hsp92II CATG 4 cut(s) 329, 378, 611, 668
HspAI GCGC 1 cut(s) 553
Kpn2I TCCGGA 1 cut(s) 209
KspAI GTTAAC 1 cut(s) 237
Kzo9I GATC 3 cut(s) 189, 362, 507
Lsp1109I GCAGC 2 cut(s) 215, 537
LweI GCATC 1 cut(s) 564
MaeI CTAG 1 cut(s) 422
MaeII ACGT 3 cut(s) 245, 342, 483
MaeIII GTNAC 3 cut(s) 220, 686, 745
MalI GATC 3 cut(s) 191, 364, 509
MboI GATC 3 cut(s) 189, 362, 507
MboII GAAGA 3 cut(s) 161, 409, 613
MfeI CAATTG 1 cut(s) 724
MflI RGATCY 2 cut(s) 362, 507
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 8 cut(s) 47, 92, 454, 542, 586, 638, 675, 724
MlyI GAGTC 2 cut(s) 188, 305
Mph1103I ATGCAT 2 cut(s) 380, 735
MroI TCCGGA 1 cut(s) 209
MseI TTAA 3 cut(s) 90, 125, 236
MspI CCGG 3 cut(s) 210, 225, 439
MunI CAATTG 1 cut(s) 724
MvnI CGCG 1 cut(s) 694
MwoI GCNNNNNNNGC 2 cut(s) 225, 559
NdeII GATC 3 cut(s) 189, 362, 507
NlaIII CATG 4 cut(s) 329, 378, 611, 668
NlaIV GGNNCC 2 cut(s) 437, 593
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 3 cut(s) 220, 686, 745
NsbI TGCGCA 1 cut(s) 554
NsiI ATGCAT 2 cut(s) 380, 735
NspI RCATGY 1 cut(s) 378
PfeI GAWTC 2 cut(s) 166, 707
PkrI GCNGC 4 cut(s) 230, 268, 523, 552
PleI GAGTC 2 cut(s) 188, 305
PmaCI CACGTG 1 cut(s) 246
PmlI CACGTG 1 cut(s) 246
PpsI GAGTC 2 cut(s) 188, 305
Ppu21I YACGTR 1 cut(s) 246
PspCI CACGTG 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 437, 593
PspPI GGNCC 3 cut(s) 441, 592, 741
PsrI GAACNNNNNNTAC 2 cut(s) 131, 163
PsuI RGATCY 2 cut(s) 362, 507
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SaqAI TTAA 3 cut(s) 90, 125, 236
SatI GCNGC 4 cut(s) 229, 267, 522, 551
Sau3AI GATC 3 cut(s) 189, 362, 507
Sau96I GGNCC 3 cut(s) 441, 592, 741
SchI GAGTC 2 cut(s) 188, 305
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 1 cut(s) 564
SinI GGWCC 2 cut(s) 441, 741
Sse9I AATT 8 cut(s) 47, 92, 454, 542, 586, 638, 675, 724
SsiI CCGC 5 cut(s) 179, 198, 267, 521, 694
SspMI CTAG 1 cut(s) 422
TaaI ACNGT 1 cut(s) 68
TaiI ACGT 3 cut(s) 248, 345, 486
TaqI TCGA 3 cut(s) 192, 309, 636
TaqII GACCGA 1 cut(s) 193
TasI AATT 8 cut(s) 47, 92, 454, 542, 586, 638, 675, 724
TauI GCSGC 2 cut(s) 269, 524
TfiI GAWTC 2 cut(s) 166, 707
Tru1I TTAA 3 cut(s) 90, 125, 236
Tru9I TTAA 3 cut(s) 90, 125, 236
TscAI CASTG 1 cut(s) 676
TseFI GTSAC 3 cut(s) 220, 686, 745
TseI GCWGC 2 cut(s) 228, 550
Tsp45I GTSAC 3 cut(s) 220, 686, 745
TspDTI ATGAA 1 cut(s) 179
TspGWI ACGGA 1 cut(s) 733
TspRI CASTG 1 cut(s) 676
VpaK11BI GGWCC 2 cut(s) 441, 741
XapI RAATTY 5 cut(s) 47, 92, 542, 638, 675
XceI RCATGY 1 cut(s) 378
XmiI GTMKAC 1 cut(s) 106
XspI CTAG 1 cut(s) 422
ZraI GACGTC 1 cut(s) 484
Zsp2I ATGCAT 2 cut(s) 380, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.