Rorug03G0179500

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001598
Physical Location & Seq
Forward (+)
15356906 .. 15359569
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0179500.1

Sequence Viewer

Length: 2664 bp
ATGAACACCGTTATCCAACCCACCTTCGCCGCCTTACCTCCTCCGCTCTCTCACTACTACCCTCCAAAACCACCACCGCACTCCTCTCTTTCTCTCTCTCCCTCTAAACCCAAACCCAAAACCCCCCTCAACTTCTCCACCTCTCTCCCCACCCAATCCCTCCAACCCCTTTTCACTACTCCACACCCTCCCAATCCCAATGGCTCTCACCAAACCCACTCTCTCTTCTCCCATCTCCTCCATCTGCTCCGCCTCTCGTCCCGCCACGCCGACGCCGAGCTCGCCAGGGCCGTCCACGCCTCCGTTCTCAAGCTCGAAAACGACACCCATCTCGGCAACGCTATTGTTTCAGCTTATCTCAAGCTGGGTCTCGTTCCCGACGCTTTCCGGGTCTTTCAGAGCCTCCCATGCCCCAATGTGGTGTCTTTCACCGCCCTCGTTTCCGGTTTTGCCAAGTCCGGCCGGGAAGAGGAGGCGGTGGAGCTTTTCTGTAGGATGAGGAGGTCAGGTATTGAGCCTAACGAGTACAGCTTTGTTGCAATTTTGACTGCTTGTATTCGGGTTTTGGACCTCGAATTGGGTCTGCAGGTTCATGCCTTGGCGGCTAAGATGGGTTACTTGGATTGTGTTTTTGTTTCCAATGCGGTGATGGGTTTGTATGGGAAATGTGGGTGTTTGGATTATGCGCTTAAGGTGTTTGATGAAATGCCTCAAAGAGACATTGCGTCTTGGAATACTGTTATGGCGAGTCTAGCGAATGAGTTTATGTATGATCAAGTGTTTGAGTTGTTCTGTGAAGTGTTGCGAGGTGATGGGTGTGTAGCGGACTATATCACGCTTTCGACTGTTTTGACTGCTTGTACTGGTAGTAATGCTTTCGTGGAAGGGCAACAAGTTCACGCCTATGCTGTCAAGACTGGTTTGGAGGGCAATTTGAGTGTTGGCAATGCACTTATTGAGTTATATGGTGAGTGTGGGAGTGTAAAAGATGTGGCTGCTTTGTTCGAGAGGATGCCTGCGAGGGATGTTATTACTTGGACGGAGATGATTACTGCCTACATGGACTTCGGGTTGGTGGGGTTGGCTGTGGAGATGTTTGATCAGATGCCAGAGAGGAATTGTTTTTCTTATAATGCTCTAATGGCTGGCTTTTGTCGAAATGGGGAAGGTTTGAGGGCACTGGAATTATTTATGAGAATGGTGGAGGAGGGTATGGAGCTGACAGAATTCACTTTGACTAGTGTTGTTGGTGCTTGTGGATTGCTCATGGACTGCAAAACTAGTGAGCAAATTCATGGGTTTGTTGTCAAGTTTGGTTTTGATTCAAATGCTTGTATTGGAGCTGCGTTGCTTGATATGTGCACAAGGTGTGATCGAATGGGAGATGCCAAGAAACTGTTTCACCAGTGGCCAAGTGAGCAGGAAAAATCTATAATTTTGACTTCACTTATATGTGGGTATACTCGAACTGGGCAACTGGATGAGGCAATTTCTATTTTCCACCGATATCAATCAGAAGGAATAATGGTTGTGGATGAAGTTGCATCAACTTCACTACTTGGTCTTTGTGGAACTGTAGGGTATCATGAACTGGGGAAACAAATCCACTCGCATGCTATCAAGTATGGTTTTCTAGCTGATGTAGGGGTAGGAAATGCCACGATGAGCATGTACACGAAGTGCTGGAACATGGATGAGGGAATCAAAATATTCAGTATGATGTCCACTCGCGACATAGTTTCATGGAATGTTTTGCTGGCTGGTTATCTTCTGCATAGACAGGGTGATGAGGCCTTAGCGGTCTGGTCAAAGATGGAGAAGACTGGCATAAAACCAGATAAGATTACCTTTATTTTGATCATTTCAGCACACAGACATACTAACTCCAATTCTGTTGAAAATTGTCGAAGTTTGTTTCTCTCCATGAAAGCAGTTTATGACATTGAGCCCACTTCAGAGCATTTTGCCTCATTTATTGGTGTTTTAGGGTACTGGGGTCTGTTGGATGAGGCAGAGGATACTATCAGTAAGATGCCCTTTGAGCCTCAAGTTTCTGTTTGGCGAGCTTTGCTTGATAGCTGTAGAATCCGTATGAATGCAACTGTTGGAAAAAGGGTTGTGAAACGTATACTTGCGATGGAGCCCAAAGATCCATCTAGCTACATACTCATATCAAATCTGTATTCAGCATCTGGGAGATGGCATTGCTCTGAAATGGTGAGAGATGACATGAGAAAAAAGGGGTTCTGGAAGCACCCAGGTCGAAGTTGGTTCATTCATAACAACAAGATACATCCATTCTATGCAAGAGATAAATCCCATCCCCAGGCGAAAGACATATATAGCGGATTAGAAATACTTATTGTAGAATGCATGAAAGCTGGTTACGTTCCAGACACAAGCTTTGTTCTTCATGAAGTGGAAGAGCATCAGAAGAAGGATTTCTTGTACTATCACAGTGCAAAACTAGCAGCCACCTATGGGCTTCTGACCAACAAACCAGGAAAACCAATTCGAGTTGTGAAGAATATCCTTCTATGTGGAGACTGCCATACCTTCTTAAAGTACTTGTCCATTGTTGCCAAGAGAGCAATACATGTAAGAGATGCTTCCGGATTTCACTATTTTTCAAATGGTCAGTGCTCATGCAAAGATTACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

887

Amino Acids

98.95

Weight (kDa)

6.56

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 137 - 167 3.7e-07 PPR repeat
PPR_2 PF13041 138 - 186 3e-12 PPR repeat family
PPR_3 PF13812 138 - 185 2.7e-07 Pentatricopeptide repeat domain
PPR PF01535 141 - 171 4.8e-08 PPR repeat
PPR_2 PF13041 373 - 419 7.2e-09 PPR repeat family
PPR PF01535 375 - 404 2.9e-07 PPR repeat
PPR_2 PF13041 577 - 623 4.3e-11 PPR repeat family
E_motif PF20431 702 - 757 5.6e-14 E motif
DYW_deaminase PF14432 795 - 887 2.5e-34 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1131
Acc36I ACCTGC 1 cut(s) 577
AccBSI CCGCTC 1 cut(s) 46
AccI GTMKAC 2 cut(s) 1460, 2128
AccII CGCG 1 cut(s) 1731
AccIII TCCGGA 1 cut(s) 2612
AclWI GGATC 1 cut(s) 2144
AcoI YGGCCR 2 cut(s) 460, 1409
AcsI RAATTY 2 cut(s) 1226, 1290
AcuI CTGAAG 1 cut(s) 1938
AcyI GRCGYC 1 cut(s) 273
AdeI CACNNNGTG 2 cut(s) 1368, 1680
AfaI GTAC 6 cut(s) 527, 862, 1673, 1991, 2450, 2567
AfiI CCNNNNNNNGG 5 cut(s) 418, 469, 577, 2326, 2481
AflII CTTAAG 1 cut(s) 689
AflIII ACRYGT 1 cut(s) 2596
AgsI TTSAA 3 cut(s) 1326, 1898, 2631
AhdI GACNNNNNGTC 1 cut(s) 724
AhlI ACTAGT 2 cut(s) 1238, 1280
AjnI CCWGG 4 cut(s) 284, 2257, 2325, 2500
Alw21I GWGCWC 3 cut(s) 282, 1364, 2645
Alw26I GTCTC 3 cut(s) 374, 711, 2538
Alw44I GTGCAC 1 cut(s) 1360
AlwI GGATC 1 cut(s) 2144
AlwNI CAGNNNCTG 1 cut(s) 2192
Aor13HI TCCGGA 1 cut(s) 2612
AoxI GGCC 4 cut(s) 288, 460, 1409, 1791
ApaLI GTGCAC 1 cut(s) 1360
ApeKI GCWGC 3 cut(s) 995, 1343, 2471
ApoI RAATTY 2 cut(s) 1226, 1290
ArsI GACNNNNNNTTYG 2 cut(s) 2387, 2419
Asp700I GAANNNNTTC 1 cut(s) 2441
AspLEI GCGC 1 cut(s) 688
AspS9I GGNCC 2 cut(s) 288, 568
AsuC2I CCSGG 2 cut(s) 389, 464
AsuHPI GGTGA 8 cut(s) 200, 421, 658, 821, 980, 1394, 1796, 2230
AvaII GGWCC 1 cut(s) 568
BaeGI GKGCMC 2 cut(s) 1180, 1364
BaeI ACNNNNGTAYC 2 cut(s) 1981, 2014
BalI TGGCCA 1 cut(s) 1411
BanII GRGCYC 3 cut(s) 282, 1950, 2145
BbsI GAAGAC 1 cut(s) 1826
Bbv12I GWGCWC 3 cut(s) 282, 1364, 2645
BbvI GCAGC 3 cut(s) 982, 1330, 2483
BceAI ACGGC 1 cut(s) 275
BcgI CGANNNNNNTGC 2 cut(s) 2243, 2277
BciT130I CCWGG 4 cut(s) 286, 2259, 2327, 2502
BciVI GTATCC 1 cut(s) 2011
BclI TGATCA 3 cut(s) 772, 1099, 1857
BcnI CCSGG 2 cut(s) 389, 464
BcoDI GTCTC 3 cut(s) 374, 711, 2538
BcuI ACTAGT 2 cut(s) 1238, 1280
BfaI CTAG 6 cut(s) 752, 1239, 1281, 1634, 2157, 2468
BfmI CTRYAG 4 cut(s) 490, 584, 1575, 2080
BfrI CTTAAG 1 cut(s) 689
BfuAI ACCTGC 1 cut(s) 577
BfuI GTATCC 1 cut(s) 2011
BglI GCCNNNNNGGC 1 cut(s) 602
BisI GCNGC 5 cut(s) 30, 603, 996, 1344, 2472
BlsI GCNGC 5 cut(s) 31, 604, 997, 1345, 2473
BmcAI AGTACT 1 cut(s) 2567
Bme1390I CCNGG 6 cut(s) 286, 389, 464, 2259, 2327, 2502
Bme18I GGWCC 1 cut(s) 568
BmeRI GACNNNNNGTC 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 288, 568
BmiI GGNNCC 1 cut(s) 2142
BmrFI CCNGG 6 cut(s) 286, 389, 464, 2259, 2327, 2502
BmrI ACTGGG 3 cut(s) 1479, 1601, 2002
BmsI GCATC 8 cut(s) 1002, 1095, 1375, 1553, 2022, 2198, 2437, 2596
BmuI ACTGGG 3 cut(s) 1479, 1601, 2002
BpiI GAAGAC 1 cut(s) 1826
Bpu10I CCTNAGC 1 cut(s) 1795
BpuEI CTTGAG 3 cut(s) 293, 344, 2031
BpuMI CCSGG 2 cut(s) 389, 464
BsaBI GATNNNNATC 1 cut(s) 1510
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 4 cut(s) 285, 597, 2257, 2325
BsaWI WCCGGW 2 cut(s) 443, 2612
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bsc4I CCNNNNNNNGG 5 cut(s) 418, 469, 577, 2326, 2481
Bse3DI GCAATG 3 cut(s) 720, 952, 2203
Bse8I GATNNNNATC 1 cut(s) 1510
BseAI TCCGGA 1 cut(s) 2612
BseBI CCWGG 4 cut(s) 286, 2259, 2327, 2502
BseDI CCNNGG 4 cut(s) 285, 597, 2257, 2325
BseGI GGATG 9 cut(s) 501, 1017, 1030, 1486, 1540, 1699, 2011, 2293, 2320
BseJI GATNNNNATC 1 cut(s) 1510
BseLI CCNNNNNNNGG 5 cut(s) 418, 469, 577, 2326, 2481
BseMI GCAATG 3 cut(s) 720, 952, 2203
BseRI GAGGAG 6 cut(s) 30, 73, 227, 485, 514, 1220
BseSI GKGCMC 2 cut(s) 1180, 1364
BseX3I CGGCCG 1 cut(s) 460
BseXI GCAGC 3 cut(s) 982, 1330, 2483
BseYI CCCAGC 1 cut(s) 364
Bsh1236I CGCG 1 cut(s) 1731
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 4 cut(s) 290, 462, 1411, 1793
BsiEI CGRYCG 1 cut(s) 463
BsiHKAI GWGCWC 3 cut(s) 282, 1364, 2645
BsiSI CCGG 5 cut(s) 388, 444, 459, 463, 2613
BslFI GGGAC 1 cut(s) 244
BslI CCNNNNNNNGG 5 cut(s) 418, 469, 577, 2326, 2481
BsmAI GTCTC 3 cut(s) 374, 711, 2538
BsmFI GGGAC 1 cut(s) 244
BsmI GAATGC 2 cut(s) 2101, 2375
BsnI GGCC 4 cut(s) 290, 462, 1411, 1793
Bso31I GGTCTC 1 cut(s) 374
Bsp1286I GDGCHC 6 cut(s) 282, 1180, 1364, 1950, 2145, 2645
Bsp13I TCCGGA 1 cut(s) 2612
Bsp1407I TGTACA 1 cut(s) 1671
Bsp143I GATC 5 cut(s) 772, 1099, 1372, 1857, 2149
Bsp68I TCGCGA 1 cut(s) 1731
BspANI GGCC 4 cut(s) 290, 462, 1411, 1793
BspEI TCCGGA 1 cut(s) 2612
BspFNI CGCG 1 cut(s) 1731
BspHI TCATGA 2 cut(s) 1585, 2413
BspLI GGNNCC 1 cut(s) 2142
BspMAI CTGCAG 1 cut(s) 588
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 1 cut(s) 2144
BspQI GCTCTTC 1 cut(s) 2418
BspTI CTTAAG 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 3 cut(s) 720, 952, 2203
BsrGI TGTACA 1 cut(s) 1671
BssECI CCNNGG 4 cut(s) 285, 597, 2257, 2325
BssMI GATC 5 cut(s) 772, 1099, 1372, 1857, 2149
BssNAI GTATAC 2 cut(s) 1461, 2129
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 1 cut(s) 597
Bst1107I GTATAC 2 cut(s) 1461, 2129
Bst2UI CCWGG 4 cut(s) 286, 2259, 2327, 2502
Bst4CI ACNGT 7 cut(s) 10, 739, 847, 1398, 1576, 2104, 2459
Bst6I CTCTTC 3 cut(s) 230, 462, 2418
BstACI GRCGYC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 689
BstAUI TGTACA 1 cut(s) 1671
BstC8I GCNNGC 6 cut(s) 282, 1017, 1147, 1614, 1758, 2064
BstDEI CTNAG 2 cut(s) 606, 1795
BstF5I GGATG 9 cut(s) 501, 1017, 1030, 1486, 1540, 1699, 2011, 2293, 2320
BstFNI CGCG 1 cut(s) 1731
BstHHI GCGC 1 cut(s) 688
BstKTI GATC 5 cut(s) 775, 1102, 1375, 1860, 2152
BstMAI GTCTC 3 cut(s) 374, 711, 2538
BstMBI GATC 5 cut(s) 772, 1099, 1372, 1857, 2149
BstMCI CGRYCG 1 cut(s) 463
BstNI CCWGG 4 cut(s) 286, 2259, 2327, 2502
BstNSI RCATGY 3 cut(s) 1616, 1672, 2600
BstSCI CCNGG 6 cut(s) 284, 387, 462, 2257, 2325, 2500
BstSFI CTRYAG 4 cut(s) 490, 584, 1575, 2080
BstSLI GKGCMC 2 cut(s) 1180, 1364
BstUI CGCG 1 cut(s) 1731
BstV1I GCAGC 3 cut(s) 982, 1330, 2483
BstV2I GAAGAC 1 cut(s) 1826
BstX2I RGATCY 1 cut(s) 2149
BstYI RGATCY 1 cut(s) 2149
BstZ17I GTATAC 2 cut(s) 1461, 2129
BstZI CGGCCG 1 cut(s) 460
BsuI GTATCC 1 cut(s) 2011
BsuRI GGCC 4 cut(s) 290, 462, 1411, 1793
BtgZI GCGATG 1 cut(s) 2150
BtsCI GGATG 9 cut(s) 501, 1017, 1030, 1486, 1540, 1699, 2011, 2293, 2320
BtsIMutI CAGTG 4 cut(s) 1178, 1412, 2464, 2645
BtuMI TCGCGA 1 cut(s) 1731
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 6 cut(s) 282, 1017, 1147, 1614, 1758, 2064
CaiI CAGNNNCTG 1 cut(s) 2192
CciI TCATGA 2 cut(s) 1585, 2413
CfoI GCGC 1 cut(s) 688
Cfr13I GGNCC 2 cut(s) 288, 568
CseI GACGC 3 cut(s) 281, 389, 714
Csp6I GTAC 6 cut(s) 526, 861, 1672, 1990, 2449, 2566
CviQI GTAC 6 cut(s) 526, 861, 1672, 1990, 2449, 2566
DdeI CTNAG 2 cut(s) 606, 1795
DpnI GATC 5 cut(s) 774, 1101, 1374, 1859, 2151
DpnII GATC 5 cut(s) 772, 1099, 1372, 1857, 2149
DraIII CACNNNGTG 2 cut(s) 1368, 1680
DriI GACNNNNNGTC 1 cut(s) 724
EaeI YGGCCR 2 cut(s) 460, 1409
EagI CGGCCG 1 cut(s) 460
Eam1104I CTCTTC 3 cut(s) 230, 462, 2418
Eam1105I GACNNNNNGTC 1 cut(s) 724
EarI CTCTTC 3 cut(s) 230, 462, 2418
EciI GGCGGA 1 cut(s) 239
Ecl136II GAGCTC 1 cut(s) 280
EclXI CGGCCG 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 597
Eco147I AGGCCT 1 cut(s) 1793
Eco24I GRGCYC 3 cut(s) 282, 1950, 2145
Eco31I GGTCTC 1 cut(s) 374
Eco32I GATATC 1 cut(s) 1508
Eco47I GGWCC 1 cut(s) 568
Eco52I CGGCCG 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 1938
EcoICRI GAGCTC 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 1226
EcoRII CCWGG 4 cut(s) 284, 2257, 2325, 2500
EcoRV GATATC 1 cut(s) 1508
EcoT14I CCWWGG 1 cut(s) 597
EcoT22I ATGCAT 1 cut(s) 2375
EcoT38I GRGCYC 3 cut(s) 282, 1950, 2145
ErhI CCWWGG 1 cut(s) 597
FalI AAGNNNNNCTT 8 cut(s) 1832, 1864, 2021, 2053, 2429, 2461, 2593, 2625
FaqI GGGAC 1 cut(s) 244
FauI CCCGC 1 cut(s) 269
FbaI TGATCA 3 cut(s) 772, 1099, 1857
FblI GTMKAC 2 cut(s) 1460, 2128
Fnu4HI GCNGC 5 cut(s) 30, 603, 996, 1344, 2472
FokI GGATG 9 cut(s) 508, 1024, 1037, 1493, 1547, 1706, 2018, 2280, 2307
FriOI GRGCYC 3 cut(s) 282, 1950, 2145
Fsp4HI GCNGC 5 cut(s) 30, 603, 996, 1344, 2472
FspBI CTAG 6 cut(s) 752, 1239, 1281, 1634, 2157, 2468
GlaI GCGC 1 cut(s) 687
GluI GCNGC 5 cut(s) 30, 603, 996, 1344, 2472
GsaI CCCAGC 1 cut(s) 368
HaeIII GGCC 4 cut(s) 290, 462, 1411, 1793
HapII CCGG 5 cut(s) 388, 444, 459, 463, 2613
HgaI GACGC 3 cut(s) 281, 389, 714
HhaI GCGC 1 cut(s) 688
Hin1I GRCGYC 1 cut(s) 273
Hin6I GCGC 1 cut(s) 686
HinP1I GCGC 1 cut(s) 686
HindIII AAGCTT 1 cut(s) 2401
HinfI GANTC 4 cut(s) 748, 1322, 1701, 2085
HpaII CCGG 5 cut(s) 388, 444, 459, 463, 2613
HphI GGTGA 8 cut(s) 200, 421, 658, 821, 980, 1394, 1796, 2230
Hpy166II GTNNAC 7 cut(s) 295, 898, 1362, 1461, 1674, 1725, 2129
Hpy188I TCNGA 7 cut(s) 399, 1104, 1516, 1957, 2212, 2433, 2490
Hpy188III TCNNGA 9 cut(s) 377, 913, 1006, 1586, 1730, 2248, 2393, 2414, 2613
Hpy8I GTNNAC 7 cut(s) 295, 898, 1362, 1461, 1674, 1725, 2129
Hpy99I CGWCG 2 cut(s) 275, 383
HpyAV CCTTC 7 cut(s) 34, 878, 1160, 1511, 2431, 2543, 2566
HpyCH4III ACNGT 7 cut(s) 10, 739, 847, 1398, 1576, 2104, 2459
HpyCH4IV ACGT 2 cut(s) 2125, 2388
HpyF3I CTNAG 2 cut(s) 606, 1795
HpySE526I ACGT 2 cut(s) 2125, 2388
Hsp92I GRCGYC 1 cut(s) 273
HspAI GCGC 1 cut(s) 686
Kpn2I TCCGGA 1 cut(s) 2612
Ksp22I TGATCA 3 cut(s) 772, 1099, 1857
Kzo9I GATC 5 cut(s) 772, 1099, 1372, 1857, 2149
LguI GCTCTTC 1 cut(s) 2418
LmnI GCTCC 5 cut(s) 252, 481, 1216, 1340, 2140
Lsp1109I GCAGC 3 cut(s) 982, 1330, 2483
LweI GCATC 8 cut(s) 1002, 1095, 1375, 1553, 2022, 2198, 2437, 2596
MaeI CTAG 6 cut(s) 752, 1239, 1281, 1634, 2157, 2468
MaeII ACGT 2 cut(s) 2125, 2388
MaeIII GTNAC 2 cut(s) 614, 2384
MalI GATC 5 cut(s) 774, 1101, 1374, 1859, 2151
MbiI CCGCTC 1 cut(s) 46
MboI GATC 5 cut(s) 772, 1099, 1372, 1857, 2149
MboII GAAGA 8 cut(s) 217, 479, 1760, 1831, 2402, 2435, 2446, 2536
MflI RGATCY 1 cut(s) 2149
MhlI GDGCHC 6 cut(s) 282, 1180, 1364, 1950, 2145, 2645
MlsI TGGCCA 1 cut(s) 1411
MluNI TGGCCA 1 cut(s) 1411
MlyI GAGTC 1 cut(s) 757
MmeI TCCRAC 4 cut(s) 40, 187, 1983, 2086
Mox20I TGGCCA 1 cut(s) 1411
Mph1103I ATGCAT 1 cut(s) 2375
MroI TCCGGA 1 cut(s) 2612
MroXI GAANNNNTTC 1 cut(s) 2441
MscI TGGCCA 1 cut(s) 1411
MseI TTAA 2 cut(s) 690, 2561
MslI CAYNNNNRTG 3 cut(s) 903, 1450, 1611
Msp20I TGGCCA 1 cut(s) 1411
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 5 cut(s) 388, 444, 459, 463, 2613
MspR9I CCNGG 6 cut(s) 286, 389, 464, 2259, 2327, 2502
Mva1269I GAATGC 2 cut(s) 2101, 2375
MvaI CCWGG 4 cut(s) 286, 2259, 2327, 2502
MvnI CGCG 1 cut(s) 1731
NciI CCSGG 2 cut(s) 389, 464
NdeII GATC 5 cut(s) 772, 1099, 1372, 1857, 2149
NlaIV GGNNCC 1 cut(s) 2142
NmeAIII GCCGAG 2 cut(s) 301, 312
NruI TCGCGA 1 cut(s) 1731
NsiI ATGCAT 1 cut(s) 2375
NspI RCATGY 3 cut(s) 1616, 1672, 2600
PaeI GCATGC 1 cut(s) 1616
PagI TCATGA 2 cut(s) 1585, 2413
PceI AGGCCT 1 cut(s) 1793
PciI ACATGT 1 cut(s) 2596
PciSI GCTCTTC 1 cut(s) 2418
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 288
PctI GAATGC 2 cut(s) 2101, 2375
PdmI GAANNNNTTC 1 cut(s) 2441
PfeI GAWTC 3 cut(s) 1322, 1701, 2085
PkrI GCNGC 5 cut(s) 31, 604, 997, 1345, 2473
PleI GAGTC 1 cut(s) 756
PpsI GAGTC 1 cut(s) 756
PscI ACATGT 1 cut(s) 2596
PsiI TTATAA 1 cut(s) 1131
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 4 cut(s) 284, 2257, 2325, 2500
PspFI CCCAGC 1 cut(s) 364
PspGI CCWGG 4 cut(s) 284, 2257, 2325, 2500
PspN4I GGNNCC 1 cut(s) 2142
PspPI GGNCC 2 cut(s) 288, 568
PstI CTGCAG 1 cut(s) 588
PstNI CAGNNNCTG 1 cut(s) 2192
PsuI RGATCY 1 cut(s) 2149
RruI TCGCGA 1 cut(s) 1731
RsaI GTAC 6 cut(s) 527, 862, 1673, 1991, 2450, 2567
RsaNI GTAC 6 cut(s) 526, 861, 1672, 1990, 2449, 2566
RseI CAYNNNNRTG 3 cut(s) 903, 1450, 1611
SacI GAGCTC 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 2418
SaqAI TTAA 2 cut(s) 690, 2561
SatI GCNGC 5 cut(s) 30, 603, 996, 1344, 2472
Sau3AI GATC 5 cut(s) 772, 1099, 1372, 1857, 2149
Sau96I GGNCC 2 cut(s) 288, 568
ScaI AGTACT 1 cut(s) 2567
SchI GAGTC 1 cut(s) 757
ScrFI CCNGG 6 cut(s) 286, 389, 464, 2259, 2327, 2502
SduI GDGCHC 6 cut(s) 282, 1180, 1364, 1950, 2145, 2645
SfaNI GCATC 8 cut(s) 1002, 1095, 1375, 1553, 2022, 2198, 2437, 2596
SfcI CTRYAG 4 cut(s) 490, 584, 1575, 2080
SinI GGWCC 1 cut(s) 568
SmiMI CAYNNNNRTG 3 cut(s) 903, 1450, 1611
SmlI CTYRAG 4 cut(s) 308, 359, 689, 2046
SmoI CTYRAG 4 cut(s) 308, 359, 689, 2046
SpeI ACTAGT 2 cut(s) 1238, 1280
SphI GCATGC 1 cut(s) 1616
SseBI AGGCCT 1 cut(s) 1793
SspI AATATT 1 cut(s) 1710
SspMI CTAG 6 cut(s) 752, 1239, 1281, 1634, 2157, 2468
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 1 cut(s) 1793
StyD4I CCNGG 6 cut(s) 284, 387, 462, 2257, 2325, 2500
StyI CCWWGG 1 cut(s) 597
TaaI ACNGT 7 cut(s) 10, 739, 847, 1398, 1576, 2104, 2459
TaiI ACGT 2 cut(s) 2128, 2391
TatI WGTACW 5 cut(s) 525, 860, 1671, 2448, 2565
TauI GCSGC 2 cut(s) 32, 605
TfiI GAWTC 3 cut(s) 1322, 1701, 2085
Tru1I TTAA 2 cut(s) 690, 2561
Tru9I TTAA 2 cut(s) 690, 2561
TscAI CASTG 4 cut(s) 1185, 1412, 2464, 2645
TseI GCWGC 3 cut(s) 995, 1343, 2471
TspGWI ACGGA 3 cut(s) 292, 1055, 2078
TspRI CASTG 4 cut(s) 1185, 1412, 2464, 2645
Vha464I CTTAAG 1 cut(s) 689
VneI GTGCAC 1 cut(s) 1360
VpaK11BI GGWCC 1 cut(s) 568
XapI RAATTY 2 cut(s) 1226, 1290
XceI RCATGY 3 cut(s) 1616, 1672, 2600
XcmI CCANNNNNNNNNTGG 2 cut(s) 646, 2265
XmiI GTMKAC 2 cut(s) 1460, 2128
XmnI GAANNNNTTC 1 cut(s) 2441
XspI CTAG 6 cut(s) 752, 1239, 1281, 1634, 2157, 2468
ZrmI AGTACT 1 cut(s) 2567
Zsp2I ATGCAT 1 cut(s) 2375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.