Prupe.7G075700_v2.0.a1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001598
Physical Location & Seq
Forward (+)
11267637 .. 11274053
6417 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G075700.9

Sequence Viewer

Length: 1254 bp
ATGGACTATGATATCTCCGACAGCAGCGGTGAGAATAGTCTGAACCTTTACAATTATCTCGAATCTGGGTCACGCATCTCAAGGGATTTCGGTACTGATGATGATCTTCCTCCATCTCATCAAAACAGAATTCCAAGAGGGGCTCACGTCACAGCAAAAGAAAGATCTGCAGTAGGTTCTATCTCATTTTCTAGGATGTATACAGACATGGAGGCCCAAATTCATCACCTAGAGCAAGAAGCATACTGTGCAGTCCTGCGTGCTTTTAAAGCTCAGTCTGATGCGATTACTTGGGAGAAGGAAAGCTTGATTACCGAACTTAGGAAAGAGCTGAGAGTATCAGATGACGAACACAGAGAACTTCTGACCAAGGTTAATTCTGATGACATCATCAGTAAGATAAGAGAGTGGAGAAAAGGAGGCGGGCATCAAGCTGCTAGGCTTAGTGCATCTCAGCCTGTCTATGATCTATTACCCAGTCCTACTGTTTCTGCGTCGCGAAAGAAACAGAAAACATCTCAATCGCATGGTCAGCCATTCCCTGGTTTATCCTCAATGAAGTCCATGCAGTACCCGTCGACAGGACCTACTTTGAGTAGACAATTAACTGGCCGTAGCTCTTCTGGTCCCCTTGTTGGAAATGAACCTGTTGAAGCAGCAACATTTGATTCATTAATCGGAAGGAAAGTATGGACCAGATGGCCTGAAGACAACAGCTTCTATGAGGCCGTTATCACTGAATACAACCGCACTGAGGGTCGACATGCATTGGTCTATGATATAAATACAGCAAACGAGACATGGGAATGGGTTGATCTCAAGGAGATCTCTCGTGAGGATATCCGTTGGGAAGGTGAGGATCCGGGCCTATCTCATCGAGGGGGCCATGGCGGTGGGCAAGGAGGACGCAGGGTTAAGAAATCTTTGAACCATGGCGGTGTTACTCCAGGTGCTGGAAGAGGACGAGGACCCATAAAGCCCCAATCAAAAAAAGAAATTCATCTGTCACAAAATGGTGTAGGGAAGAAGATGCCAGATGATCTGGAATTATTAAATACTGATACTCTGGTAAAGGAGGTGGAGAGAGTTTTCAATGCAAATCATCCAGATCCTTTTGAGCTTGAAAAAGCAAGGAAAATGCTGAAAGATCACGAACAGGCCCTTGTTGATGCCATCGCAAGGCTTGCATATGCATCGGATGGCGAAAGTGATGGAGAGCATACGTTTCTGCATGGTCGATCAATGGATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

418

Amino Acids

46.5

Weight (kDa)

6.08

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 542, 953
AccI GTMKAC 4 cut(s) 200, 578, 598, 760
AccII CGCG 1 cut(s) 499
AciI CCGC 5 cut(s) 27, 423, 748, 891, 936
AclWI GGATC 3 cut(s) 854, 867, 1103
AcoI YGGCCR 1 cut(s) 610
AcsI RAATTY 3 cut(s) 129, 219, 996
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 2 cut(s) 94, 572
AfiI CCNNNNNNNGG 7 cut(s) 321, 542, 581, 635, 754, 953, 1179
AgsI TTSAA 4 cut(s) 653, 928, 1093, 1124
AjiI CACGTC 1 cut(s) 148
AjnI CCWGG 2 cut(s) 541, 946
AluBI AGCT 7 cut(s) 272, 306, 331, 434, 618, 717, 1120
AluI AGCT 7 cut(s) 272, 306, 331, 434, 618, 717, 1120
Alw26I GTCTC 1 cut(s) 791
AlwI GGATC 3 cut(s) 854, 867, 1103
AlwNI CAGNNNCTG 1 cut(s) 953
AoxI GGCC 7 cut(s) 213, 610, 701, 726, 865, 883, 1158
ApeKI GCWGC 3 cut(s) 24, 434, 656
ApoI RAATTY 3 cut(s) 129, 219, 996
AseI ATTAAT 1 cut(s) 674
AspS9I GGNCC 8 cut(s) 214, 584, 626, 693, 865, 883, 968, 1159
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 3 cut(s) 41, 218, 866
AvaII GGWCC 4 cut(s) 584, 626, 693, 968
BaeI ACNNNNGTAYC 2 cut(s) 1053, 1086
BamHI GGATCC 1 cut(s) 859
BanII GRGCYC 1 cut(s) 145
BauI CACGAG 1 cut(s) 831
BbsI GAAGAC 1 cut(s) 714
BbvI GCAGC 3 cut(s) 36, 421, 668
BccI CCATC 6 cut(s) 121, 693, 1181, 1193, 1205, 1241
BceAI ACGGC 2 cut(s) 597, 713
BcgI CGANNNNNNTGC 2 cut(s) 1176, 1210
BciT130I CCWGG 2 cut(s) 543, 948
BcnI CCSGG 1 cut(s) 864
BcoDI GTCTC 1 cut(s) 791
BfaI CTAG 3 cut(s) 192, 230, 438
BfmI CTRYAG 1 cut(s) 168
BglII AGATCT 2 cut(s) 164, 825
BisI GCNGC 3 cut(s) 25, 435, 657
BlsI GCNGC 3 cut(s) 26, 436, 658
Bme1390I CCNGG 3 cut(s) 543, 864, 948
Bme18I GGWCC 4 cut(s) 584, 626, 693, 968
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 8 cut(s) 214, 584, 626, 693, 865, 883, 968, 1159
BmiI GGNNCC 4 cut(s) 628, 861, 884, 970
BmrFI CCNGG 3 cut(s) 543, 864, 948
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 7 cut(s) 84, 271, 436, 458, 1020, 1159, 1202
BmuI ACTGGG 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 714
BpmI CTGGAG 1 cut(s) 930
BpuEI CTTGAG 2 cut(s) 64, 803
BpuMI CCSGG 1 cut(s) 864
BsaBI GATNNNNATC 1 cut(s) 102
BsaJI CCNNGG 4 cut(s) 369, 541, 886, 931
Bsc4I CCNNNNNNNGG 7 cut(s) 321, 542, 581, 635, 754, 953, 1179
Bse1I ACTGG 2 cut(s) 477, 613
Bse8I GATNNNNATC 1 cut(s) 102
BseBI CCWGG 2 cut(s) 543, 948
BseDI CCNNGG 4 cut(s) 369, 541, 886, 931
BseGI GGATG 4 cut(s) 201, 1102, 1204, 1252
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 7 cut(s) 321, 542, 581, 635, 754, 953, 1179
BseMII CTCAG 4 cut(s) 287, 323, 467, 744
BseNI ACTGG 2 cut(s) 477, 613
BseXI GCAGC 3 cut(s) 36, 421, 668
BsgI GTGCAG 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 499
BshFI GGCC 7 cut(s) 215, 612, 703, 728, 867, 885, 1160
BsiSI CCGG 1 cut(s) 863
BslFI GGGAC 1 cut(s) 612
BslI CCNNNNNNNGG 7 cut(s) 321, 542, 581, 635, 754, 953, 1179
BsmAI GTCTC 1 cut(s) 791
BsmFI GGGAC 1 cut(s) 612
BsnI GGCC 7 cut(s) 215, 612, 703, 728, 867, 885, 1160
Bsp1286I GDGCHC 1 cut(s) 145
Bsp19I CCATGG 2 cut(s) 886, 931
Bsp68I TCGCGA 1 cut(s) 499
BspACI CCGC 5 cut(s) 27, 423, 748, 891, 936
BspANI GGCC 7 cut(s) 215, 612, 703, 728, 867, 885, 1160
BspCNI CTCAG 4 cut(s) 286, 324, 466, 745
BspFNI CGCG 1 cut(s) 499
BspLI GGNNCC 4 cut(s) 628, 861, 884, 970
BspMAI CTGCAG 1 cut(s) 172
BspPI GGATC 3 cut(s) 854, 867, 1103
BspQI GCTCTTC 1 cut(s) 625
BsrI ACTGG 2 cut(s) 477, 613
BssECI CCNNGG 4 cut(s) 369, 541, 886, 931
BssNAI GTATAC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 831
BssT1I CCWWGG 3 cut(s) 369, 886, 931
Bst1107I GTATAC 1 cut(s) 201
Bst2BI CACGAG 1 cut(s) 831
Bst2UI CCWGG 2 cut(s) 543, 948
Bst4CI ACNGT 2 cut(s) 248, 487
Bst6I CTCTTC 2 cut(s) 625, 952
BstAPI GCANNNNNTGC 2 cut(s) 248, 1184
BstC8I GCNNGC 3 cut(s) 261, 425, 1185
BstDEI CTNAG 6 cut(s) 273, 320, 332, 443, 453, 753
BstDSI CCRYGG 2 cut(s) 886, 931
BstF5I GGATG 4 cut(s) 201, 1102, 1204, 1252
BstFNI CGCG 1 cut(s) 499
BstMAI GTCTC 1 cut(s) 791
BstMWI GCNNNNNNNGC 4 cut(s) 248, 269, 532, 1184
BstNI CCWGG 2 cut(s) 543, 948
BstNSI RCATGY 1 cut(s) 767
BstSCI CCNGG 3 cut(s) 541, 862, 946
BstSFI CTRYAG 1 cut(s) 168
BstUI CGCG 1 cut(s) 499
BstV1I GCAGC 3 cut(s) 36, 421, 668
BstV2I GAAGAC 1 cut(s) 714
BstX2I RGATCY 4 cut(s) 164, 825, 859, 1108
BstXI CCANNNNNNTGG 1 cut(s) 893
BstYI RGATCY 4 cut(s) 164, 825, 859, 1108
BstZ17I GTATAC 1 cut(s) 201
BsuRI GGCC 7 cut(s) 215, 612, 703, 728, 867, 885, 1160
BtgI CCRYGG 2 cut(s) 886, 931
BtgZI GCGATG 1 cut(s) 1159
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 4 cut(s) 201, 1102, 1204, 1252
BtsIMutI CAGTG 2 cut(s) 735, 750
BtuMI TCGCGA 1 cut(s) 499
Cac8I GCNNGC 3 cut(s) 261, 425, 1185
CaiI CAGNNNCTG 1 cut(s) 953
Cfr13I GGNCC 8 cut(s) 214, 584, 626, 693, 865, 883, 968, 1159
CseI GACGC 2 cut(s) 483, 915
Csp6I GTAC 2 cut(s) 93, 571
CviAII CATG 8 cut(s) 208, 527, 565, 764, 801, 887, 932, 1232
CviQI GTAC 2 cut(s) 93, 571
DdeI CTNAG 6 cut(s) 273, 320, 332, 443, 453, 753
DraI TTTAAA 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 610
Eam1104I CTCTTC 2 cut(s) 625, 952
EarI CTCTTC 2 cut(s) 625, 952
Eco130I CCWWGG 3 cut(s) 369, 886, 931
Eco24I GRGCYC 1 cut(s) 145
Eco32I GATATC 2 cut(s) 13, 841
Eco47I GGWCC 4 cut(s) 584, 626, 693, 968
Eco57I CTGAAG 1 cut(s) 726
EcoO109I RGGNCCY 3 cut(s) 584, 968, 1159
EcoRI GAATTC 1 cut(s) 129
EcoRII CCWGG 2 cut(s) 541, 946
EcoRV GATATC 2 cut(s) 13, 841
EcoT14I CCWWGG 3 cut(s) 369, 886, 931
EcoT22I ATGCAT 2 cut(s) 769, 1195
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 3 cut(s) 369, 886, 931
FaeI CATG 8 cut(s) 211, 530, 568, 767, 804, 890, 935, 1235
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 1 cut(s) 612
FatI CATG 8 cut(s) 207, 526, 564, 763, 800, 886, 931, 1231
FauI CCCGC 1 cut(s) 416
FauNDI CATATG 1 cut(s) 1189
FblI GTMKAC 4 cut(s) 200, 578, 598, 760
Fnu4HI GCNGC 3 cut(s) 25, 435, 657
FokI GGATG 3 cut(s) 208, 1089, 1211
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 3 cut(s) 25, 435, 657
FspBI CTAG 3 cut(s) 192, 230, 438
GluI GCNGC 3 cut(s) 25, 435, 657
GsuI CTGGAG 1 cut(s) 930
HaeIII GGCC 7 cut(s) 215, 612, 703, 728, 867, 885, 1160
HapII CCGG 1 cut(s) 863
HgaI GACGC 2 cut(s) 483, 915
Hin1II CATG 8 cut(s) 211, 530, 568, 767, 804, 890, 935, 1235
HincII GTYRAC 2 cut(s) 579, 761
HindII GTYRAC 2 cut(s) 579, 761
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 2 cut(s) 62, 668
HpaII CCGG 1 cut(s) 863
HphI GGTGA 3 cut(s) 41, 218, 866
Hpy166II GTNNAC 4 cut(s) 201, 579, 599, 761
Hpy188I TCNGA 8 cut(s) 19, 42, 280, 343, 366, 382, 680, 1198
Hpy188III TCNNGA 6 cut(s) 59, 498, 833, 1043, 1106, 1151
Hpy8I GTNNAC 4 cut(s) 201, 579, 599, 761
Hpy99I CGWCG 2 cut(s) 499, 580
HpyAV CCTTC 3 cut(s) 292, 675, 845
HpyCH4III ACNGT 2 cut(s) 248, 487
HpyCH4IV ACGT 2 cut(s) 147, 1223
HpyCH4V TGCA 9 cut(s) 170, 251, 449, 568, 767, 1097, 1187, 1193, 1231
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 269, 532, 1184
HpyF3I CTNAG 6 cut(s) 273, 320, 332, 443, 453, 753
HpySE526I ACGT 2 cut(s) 147, 1223
Hsp92II CATG 8 cut(s) 211, 530, 568, 767, 804, 890, 935, 1235
LguI GCTCTTC 1 cut(s) 625
Lsp1109I GCAGC 3 cut(s) 36, 421, 668
LweI GCATC 7 cut(s) 84, 271, 436, 458, 1020, 1159, 1202
MaeI CTAG 3 cut(s) 192, 230, 438
MaeII ACGT 2 cut(s) 147, 1223
MaeIII GTNAC 4 cut(s) 69, 148, 940, 1005
MboII GAAGA 6 cut(s) 98, 612, 719, 969, 1036, 1039
MflI RGATCY 4 cut(s) 164, 825, 859, 1108
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 7 cut(s) 52, 129, 219, 376, 602, 996, 1046
MmeI TCCRAC 2 cut(s) 42, 616
Mph1103I ATGCAT 2 cut(s) 769, 1195
MseI TTAA 6 cut(s) 267, 375, 605, 674, 915, 1052
MslI CAYNNNNRTG 3 cut(s) 805, 891, 936
MspA1I CMGCKG 1 cut(s) 27
MspI CCGG 1 cut(s) 863
MspR9I CCNGG 3 cut(s) 543, 864, 948
MvaI CCWGG 2 cut(s) 543, 948
MvnI CGCG 1 cut(s) 499
MwoI GCNNNNNNNGC 4 cut(s) 248, 269, 532, 1184
NciI CCSGG 1 cut(s) 864
NcoI CCATGG 2 cut(s) 886, 931
NdeI CATATG 1 cut(s) 1189
NlaIII CATG 8 cut(s) 211, 530, 568, 767, 804, 890, 935, 1235
NlaIV GGNNCC 4 cut(s) 628, 861, 884, 970
NmuCI GTSAC 3 cut(s) 69, 148, 1005
NruI TCGCGA 1 cut(s) 499
NsiI ATGCAT 2 cut(s) 769, 1195
NspI RCATGY 1 cut(s) 767
PciSI GCTCTTC 1 cut(s) 625
PfeI GAWTC 2 cut(s) 62, 668
PflMI CCANNNNNTGG 2 cut(s) 542, 953
PkrI GCNGC 3 cut(s) 26, 436, 658
PpuMI RGGWCCY 2 cut(s) 584, 968
PshBI ATTAAT 1 cut(s) 674
Psp5II RGGWCCY 2 cut(s) 584, 968
Psp6I CCWGG 2 cut(s) 541, 946
PspGI CCWGG 2 cut(s) 541, 946
PspN4I GGNNCC 4 cut(s) 628, 861, 884, 970
PspPI GGNCC 8 cut(s) 214, 584, 626, 693, 865, 883, 968, 1159
PspPPI RGGWCCY 2 cut(s) 584, 968
PstI CTGCAG 1 cut(s) 172
PstNI CAGNNNCTG 1 cut(s) 953
PsuI RGATCY 4 cut(s) 164, 825, 859, 1108
RruI TCGCGA 1 cut(s) 499
RsaI GTAC 2 cut(s) 94, 572
RsaNI GTAC 2 cut(s) 93, 571
RseI CAYNNNNRTG 3 cut(s) 805, 891, 936
SalI GTCGAC 2 cut(s) 577, 759
SapI GCTCTTC 1 cut(s) 625
SaqAI TTAA 6 cut(s) 267, 375, 605, 674, 915, 1052
SatI GCNGC 3 cut(s) 25, 435, 657
Sau96I GGNCC 8 cut(s) 214, 584, 626, 693, 865, 883, 968, 1159
ScrFI CCNGG 3 cut(s) 543, 864, 948
SduI GDGCHC 1 cut(s) 145
SfaNI GCATC 7 cut(s) 84, 271, 436, 458, 1020, 1159, 1202
SfcI CTRYAG 1 cut(s) 168
SinI GGWCC 4 cut(s) 584, 626, 693, 968
SmiMI CAYNNNNRTG 3 cut(s) 805, 891, 936
SmlI CTYRAG 2 cut(s) 79, 818
SmoI CTYRAG 2 cut(s) 79, 818
Sse9I AATT 7 cut(s) 52, 129, 219, 376, 602, 996, 1046
SsiI CCGC 5 cut(s) 27, 423, 748, 891, 936
SspMI CTAG 3 cut(s) 192, 230, 438
StyD4I CCNGG 3 cut(s) 541, 862, 946
StyI CCWWGG 3 cut(s) 369, 886, 931
TaaI ACNGT 2 cut(s) 248, 487
TaiI ACGT 2 cut(s) 150, 1226
TaqI TCGA 5 cut(s) 60, 578, 760, 877, 1237
TasI AATT 7 cut(s) 52, 129, 219, 376, 602, 996, 1046
TfiI GAWTC 2 cut(s) 62, 668
Tru1I TTAA 6 cut(s) 267, 375, 605, 674, 915, 1052
Tru9I TTAA 6 cut(s) 267, 375, 605, 674, 915, 1052
TscAI CASTG 2 cut(s) 742, 757
TseFI GTSAC 3 cut(s) 69, 148, 1005
TseI GCWGC 3 cut(s) 24, 434, 656
Tsp45I GTSAC 3 cut(s) 69, 148, 1005
TspDTI ATGAA 5 cut(s) 212, 572, 657, 660, 989
TspGWI ACGGA 1 cut(s) 833
TspRI CASTG 2 cut(s) 742, 757
Van91I CCANNNNNTGG 2 cut(s) 542, 953
VpaK11BI GGWCC 4 cut(s) 584, 626, 693, 968
VspI ATTAAT 1 cut(s) 674
XapI RAATTY 3 cut(s) 129, 219, 996
XceI RCATGY 1 cut(s) 767
XmiI GTMKAC 4 cut(s) 200, 578, 598, 760
XspI CTAG 3 cut(s) 192, 230, 438
Zsp2I ATGCAT 2 cut(s) 769, 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.