Rh3BG263000

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001598
Physical Location & Seq
Reverse (-)
25469728 .. 25475365
5638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG263000.1

Sequence Viewer

Length: 1026 bp
ATGGAGGCCCAAATTCATCACCTAGAGCAAGAAGCATACTGTGCAGTCCTGCGTGCTTTCAAAGCTCAGTCTGATGCGATTACTTGGGAGAAGGAAGGTTTGATTACAGAACTTAGGAAAGAGCTGAGAGTATCCGATGATGAACACAGAGAGCTTCTTGGCAGGGTTAACTCTGATGATATCATCAGTAAGATAAGAGAGTGGAGAACGGCAGGTGGGCATCAAGCTGCGAGATTCAGTGCATCCCAGCCTGTTTATGACCTTGTATCCAGTCCTACTGTTTCAGCATCTCGAAAGAAACAGAAAACATCCCAATCGCAGGGTCAGCCATTCCCTGGTTTATCCTCAATGAAGTCTATGCAGTACCCTTCTACAGGACCTTCGTTGAGTAGGCAGCTCACTAGCCGCAGTACTACCGGTCCCCTTGCCATAACTGAACCTGCTGTAGCAGCAACATATGATTTAATTGGAAAAAAAGTGTGGATGAGATGGCCTGCAGACAACAGCTTCTATGAAGCCCTTATAACAGACTACAACCCTAATGATGGTCGACATGCTTTGATCTACGATAAAAATACAGCAAATGAGACATTTGAATGGGTAAATTTCAAAGAGATTTCTCGTGAGGATATCCGTTGGGAAGGTGAGGAACTGGGGCCATCTCATCAAGGGAACCATGGCAGCGGACAAGGACGTAGGGTTAAGAAATCTACGAACCATGGTGGTGTTGGTCCAGTTTCTGGGAGAGGTCGAGGACATATAAAGCCTCAATCAAAGAAACAACTTCTTCCTTCACAAAATGGCATAGGAAATAAGTTGCCAGATGATCTAGAATTATTGAATACTGATTCTCTGGTAAAGGAGGTGGAGAGAGTTTTCAATGCAAGTAATCCGGACCCCTTTGAGCTTGAAAAGGCAAGGAAAATGCTGAAAGATCACGAACAGGCACTTGTTGATGCCATTGAAAGGCTTGCATATGCATCGGATGGTGAAAGTGATGGAGAGCATGCATTTATGCATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

341

Amino Acids

38.03

Weight (kDa)

6.33

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 3 - 71 3.7e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AarI CACCTGC 1 cut(s) 203
Acc36I ACCTGC 2 cut(s) 203, 448
AccB7I CCANNNNNTGG 2 cut(s) 335, 740
AccI GTMKAC 1 cut(s) 550
AccIII TCCGGA 1 cut(s) 892
AciI CCGC 2 cut(s) 406, 684
AcsI RAATTY 2 cut(s) 12, 604
AfaI GTAC 2 cut(s) 365, 412
AfiI CCNNNNNNNGG 6 cut(s) 319, 335, 374, 545, 740, 966
AgeI ACCGGT 1 cut(s) 416
AgsI TTSAA 7 cut(s) 61, 596, 610, 841, 880, 911, 965
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 7 cut(s) 65, 124, 154, 227, 397, 507, 907
AluI AGCT 7 cut(s) 65, 124, 154, 227, 397, 507, 907
Alw26I GTCTC 1 cut(s) 581
AlwNI CAGNNNCTG 1 cut(s) 740
Aor13HI TCCGGA 1 cut(s) 892
AoxI GGCC 3 cut(s) 6, 491, 656
ApeKI GCWGC 4 cut(s) 227, 394, 449, 681
ApoI RAATTY 2 cut(s) 12, 604
AsiGI ACCGGT 1 cut(s) 416
AspS9I GGNCC 6 cut(s) 7, 377, 419, 656, 731, 895
AsuHPI GGTGA 3 cut(s) 11, 656, 1001
AvaII GGWCC 4 cut(s) 377, 419, 731, 895
BauI CACGAG 1 cut(s) 621
BbvI GCAGC 4 cut(s) 214, 406, 461, 693
BccI CCATC 5 cut(s) 483, 539, 667, 980, 992
BceAI ACGGC 1 cut(s) 225
BcgI CGANNNNNNTGC 2 cut(s) 963, 997
BciT130I CCWGG 1 cut(s) 336
BciVI GTATCC 2 cut(s) 142, 277
BcoDI GTCTC 1 cut(s) 581
BfaI CTAG 3 cut(s) 23, 402, 830
BfmI CTRYAG 3 cut(s) 372, 444, 495
BfuAI ACCTGC 2 cut(s) 203, 448
BfuI GTATCC 2 cut(s) 142, 277
BisI GCNGC 5 cut(s) 228, 395, 406, 450, 682
BlsI GCNGC 5 cut(s) 229, 396, 407, 451, 683
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 1 cut(s) 336
Bme18I GGWCC 4 cut(s) 377, 419, 731, 895
BmgT120I GGNCC 6 cut(s) 7, 377, 419, 656, 731, 895
BmiI GGNNCC 4 cut(s) 421, 657, 674, 897
BmrFI CCNGG 1 cut(s) 336
BmrI ACTGGG 1 cut(s) 662
BmsI GCATC 6 cut(s) 64, 229, 251, 296, 946, 989
BmuI ACTGGG 1 cut(s) 662
BsaJI CCNNGG 3 cut(s) 334, 676, 718
BsaWI WCCGGW 2 cut(s) 416, 892
Bsc4I CCNNNNNNNGG 6 cut(s) 319, 335, 374, 545, 740, 966
Bse118I RCCGGY 1 cut(s) 416
Bse1I ACTGG 3 cut(s) 270, 657, 734
BseAI TCCGGA 1 cut(s) 892
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 3 cut(s) 334, 676, 718
BseGI GGATG 4 cut(s) 242, 308, 489, 991
BseLI CCNNNNNNNGG 6 cut(s) 319, 335, 374, 545, 740, 966
BseMII CTCAG 2 cut(s) 80, 116
BseNI ACTGG 3 cut(s) 270, 657, 734
BseXI GCAGC 4 cut(s) 214, 406, 461, 693
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 3 cut(s) 8, 493, 658
BshTI ACCGGT 1 cut(s) 416
BsiSI CCGG 2 cut(s) 417, 893
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 6 cut(s) 319, 335, 374, 545, 740, 966
BsmAI GTCTC 1 cut(s) 581
BsmFI GGGAC 1 cut(s) 405
BsnI GGCC 3 cut(s) 8, 493, 658
Bsp13I TCCGGA 1 cut(s) 892
Bsp143I GATC 3 cut(s) 561, 826, 934
Bsp19I CCATGG 2 cut(s) 676, 718
BspACI CCGC 2 cut(s) 406, 684
BspANI GGCC 3 cut(s) 8, 493, 658
BspCNI CTCAG 2 cut(s) 79, 117
BspEI TCCGGA 1 cut(s) 892
BspLI GGNNCC 4 cut(s) 421, 657, 674, 897
BspMAI CTGCAG 1 cut(s) 499
BspMI ACCTGC 2 cut(s) 203, 448
BsrFI RCCGGY 1 cut(s) 416
BsrI ACTGG 3 cut(s) 270, 657, 734
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 3 cut(s) 334, 676, 718
BssMI GATC 3 cut(s) 561, 826, 934
BssSI CACGAG 1 cut(s) 621
BssT1I CCWWGG 2 cut(s) 676, 718
Bst2BI CACGAG 1 cut(s) 621
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 2 cut(s) 41, 280
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 4 cut(s) 54, 495, 972, 1008
BstDEI CTNAG 3 cut(s) 66, 113, 125
BstDSI CCRYGG 2 cut(s) 676, 718
BstENI CCTNNNNNAGG 1 cut(s) 372
BstF5I GGATG 4 cut(s) 242, 308, 489, 991
BstKTI GATC 3 cut(s) 564, 829, 937
BstMAI GTCTC 1 cut(s) 581
BstMBI GATC 3 cut(s) 561, 826, 934
BstMWI GCNNNNNNNGC 4 cut(s) 41, 62, 325, 449
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 2 cut(s) 557, 1010
BstSCI CCNGG 1 cut(s) 334
BstSFI CTRYAG 3 cut(s) 372, 444, 495
BstV1I GCAGC 4 cut(s) 214, 406, 461, 693
BsuI GTATCC 2 cut(s) 142, 277
BsuRI GGCC 3 cut(s) 8, 493, 658
BtgI CCRYGG 2 cut(s) 676, 718
BtsCI GGATG 4 cut(s) 242, 308, 489, 991
BtsIMutI CAGTG 1 cut(s) 244
BveI ACCTGC 2 cut(s) 203, 448
Cac8I GCNNGC 4 cut(s) 54, 495, 972, 1008
CaiI CAGNNNCTG 1 cut(s) 740
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 6 cut(s) 7, 377, 419, 656, 731, 895
Csp6I GTAC 2 cut(s) 364, 411
CspAI ACCGGT 1 cut(s) 416
CviAII CATG 5 cut(s) 554, 677, 719, 1007, 1019
CviQI GTAC 2 cut(s) 364, 411
DdeI CTNAG 3 cut(s) 66, 113, 125
DpnI GATC 3 cut(s) 563, 828, 936
DpnII GATC 3 cut(s) 561, 826, 934
Eco130I CCWWGG 2 cut(s) 676, 718
Eco32I GATATC 2 cut(s) 181, 631
Eco47I GGWCC 4 cut(s) 377, 419, 731, 895
EcoNI CCTNNNNNAGG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 334
EcoRV GATATC 2 cut(s) 181, 631
EcoT14I CCWWGG 2 cut(s) 676, 718
EcoT22I ATGCAT 3 cut(s) 982, 1012, 1020
ErhI CCWWGG 2 cut(s) 676, 718
FaeI CATG 5 cut(s) 557, 680, 722, 1010, 1022
FaqI GGGAC 1 cut(s) 405
FatI CATG 5 cut(s) 553, 676, 718, 1006, 1018
FauNDI CATATG 2 cut(s) 457, 976
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 5 cut(s) 228, 395, 406, 450, 682
FokI GGATG 4 cut(s) 229, 295, 496, 998
Fsp4HI GCNGC 5 cut(s) 228, 395, 406, 450, 682
FspBI CTAG 3 cut(s) 23, 402, 830
GluI GCNGC 5 cut(s) 228, 395, 406, 450, 682
GsaI CCCAGC 1 cut(s) 250
HaeIII GGCC 3 cut(s) 8, 493, 658
HapII CCGG 2 cut(s) 417, 893
Hin1II CATG 5 cut(s) 557, 680, 722, 1010, 1022
HincII GTYRAC 2 cut(s) 169, 551
HindII GTYRAC 2 cut(s) 169, 551
HinfI GANTC 2 cut(s) 234, 848
HpaI GTTAAC 1 cut(s) 169
HpaII CCGG 2 cut(s) 417, 893
HphI GGTGA 3 cut(s) 11, 656, 1001
Hpy166II GTNNAC 2 cut(s) 169, 551
Hpy188I TCNGA 4 cut(s) 73, 136, 175, 985
Hpy188III TCNNGA 5 cut(s) 291, 623, 830, 893, 938
Hpy8I GTNNAC 2 cut(s) 169, 551
HpyAV CCTTC 6 cut(s) 85, 89, 378, 390, 635, 801
HpyCH4III ACNGT 2 cut(s) 41, 280
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 9 cut(s) 44, 242, 361, 497, 884, 974, 980, 1010, 1018
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 62, 325, 449
HpyF3I CTNAG 3 cut(s) 66, 113, 125
HpySE526I ACGT 1 cut(s) 694
Hsp92II CATG 5 cut(s) 557, 680, 722, 1010, 1022
Kpn2I TCCGGA 1 cut(s) 892
KspAI GTTAAC 1 cut(s) 169
Kzo9I GATC 3 cut(s) 561, 826, 934
Lsp1109I GCAGC 4 cut(s) 214, 406, 461, 693
LweI GCATC 6 cut(s) 64, 229, 251, 296, 946, 989
MaeI CTAG 3 cut(s) 23, 402, 830
MaeII ACGT 1 cut(s) 694
MalI GATC 3 cut(s) 563, 828, 936
MboI GATC 3 cut(s) 561, 826, 934
MboII GAAGA 1 cut(s) 779
MluCI AATT 4 cut(s) 12, 465, 604, 833
MnlI CCTC 7 cut(s) 355, 619, 640, 740, 746, 777, 856
Mph1103I ATGCAT 3 cut(s) 982, 1012, 1020
MroI TCCGGA 1 cut(s) 892
MseI TTAA 3 cut(s) 168, 464, 702
MslI CAYNNNNRTG 2 cut(s) 595, 723
MspA1I CMGCKG 1 cut(s) 684
MspI CCGG 2 cut(s) 417, 893
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 4 cut(s) 41, 62, 325, 449
NcoI CCATGG 2 cut(s) 676, 718
NdeI CATATG 2 cut(s) 457, 976
NdeII GATC 3 cut(s) 561, 826, 934
NlaIII CATG 5 cut(s) 557, 680, 722, 1010, 1022
NlaIV GGNNCC 4 cut(s) 421, 657, 674, 897
NsiI ATGCAT 3 cut(s) 982, 1012, 1020
NspI RCATGY 2 cut(s) 557, 1010
PaeI GCATGC 1 cut(s) 1010
PaqCI CACCTGC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 234, 848
PflMI CCANNNNNTGG 2 cut(s) 335, 740
PinAI ACCGGT 1 cut(s) 416
PkrI GCNGC 5 cut(s) 229, 396, 407, 451, 683
PpuMI RGGWCCY 1 cut(s) 377
PsiI TTATAA 1 cut(s) 524
Psp5II RGGWCCY 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 334
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 4 cut(s) 421, 657, 674, 897
PspPI GGNCC 6 cut(s) 7, 377, 419, 656, 731, 895
PspPPI RGGWCCY 1 cut(s) 377
PstI CTGCAG 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 740
RsaI GTAC 2 cut(s) 365, 412
RsaNI GTAC 2 cut(s) 364, 411
RseI CAYNNNNRTG 2 cut(s) 595, 723
SalI GTCGAC 1 cut(s) 549
SaqAI TTAA 3 cut(s) 168, 464, 702
SatI GCNGC 5 cut(s) 228, 395, 406, 450, 682
Sau3AI GATC 3 cut(s) 561, 826, 934
Sau96I GGNCC 6 cut(s) 7, 377, 419, 656, 731, 895
ScaI AGTACT 1 cut(s) 412
ScrFI CCNGG 1 cut(s) 336
SfaNI GCATC 6 cut(s) 64, 229, 251, 296, 946, 989
SfcI CTRYAG 3 cut(s) 372, 444, 495
SinI GGWCC 4 cut(s) 377, 419, 731, 895
SmiMI CAYNNNNRTG 2 cut(s) 595, 723
SphI GCATGC 1 cut(s) 1010
Sse9I AATT 4 cut(s) 12, 465, 604, 833
SsiI CCGC 2 cut(s) 406, 684
SspMI CTAG 3 cut(s) 23, 402, 830
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 2 cut(s) 676, 718
TaaI ACNGT 2 cut(s) 41, 280
TaiI ACGT 1 cut(s) 697
TaqI TCGA 3 cut(s) 292, 550, 751
TasI AATT 4 cut(s) 12, 465, 604, 833
TatI WGTACW 1 cut(s) 410
TauI GCSGC 1 cut(s) 408
TfiI GAWTC 2 cut(s) 234, 848
Tru1I TTAA 3 cut(s) 168, 464, 702
Tru9I TTAA 3 cut(s) 168, 464, 702
TscAI CASTG 1 cut(s) 244
TseI GCWGC 4 cut(s) 227, 394, 449, 681
TspDTI ATGAA 4 cut(s) 5, 156, 365, 528
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 1 cut(s) 244
Van91I CCANNNNNTGG 2 cut(s) 335, 740
VpaK11BI GGWCC 4 cut(s) 377, 419, 731, 895
XagI CCTNNNNNAGG 1 cut(s) 372
XapI RAATTY 2 cut(s) 12, 604
XbaI TCTAGA 1 cut(s) 829
XceI RCATGY 2 cut(s) 557, 1010
XcmI CCANNNNNNNNNTGG 1 cut(s) 725
XmiI GTMKAC 1 cut(s) 550
XspI CTAG 3 cut(s) 23, 402, 830
ZrmI AGTACT 1 cut(s) 412
Zsp2I ATGCAT 3 cut(s) 982, 1012, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.