MD14G1059100.v1.1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001598
Physical Location & Seq
Reverse (-)
6082536 .. 6087165
4630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1059100.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGACTATGATGTCTCCGAAATCAGTGGCACTGATGATGATCTTCCTCCATCTCATCAAAACAGAATTCCTCGAGGGGGTAAAGTCTCAGGAAACGAAAGATCTGCTGTAGGTTCTATCTCATTTTCTAGGATGTATACAGACATGGAGGCTGAAATTCATCACCTTGAGCAAGAAGCATACTGTGCAGTCCTAAGGGCCTTTAAAGCGCAGTCTAATGCGATTACTTGGGAGAAGGAAGGTTTGATTACTGAACTTAGGAAAGAACTGAGAGTATCGGATGATGAACACAGAGAGCTTCTGACCAGGGTTAATTCTGAGGACATCATCGATAAGATAAGAGTGTGGAGAGACAAAGGTGGTCATCAAGCTGCTAGGCTTACTTCATCTCAGAATGTAAATGTATTTGAACTTTTATCTAGTCCTACTATTTCAGCGTCCCGAAAGAAACAGAAAACATCACAATCGCATGGTCAGCCATTCCCTGGTTTATCCTCAATGAAGTCCATGAAATACCCTTCAACAGGACCTGTTGGAAGTAGACAATTCACCCGCAGTTCTTCTGGTCCCTTTGTTGAAGCGGCTGAAGCAGAAACATCTGATTCATTAGTTGGAAGGAAAGTGTGGACAAGGTGGCCTGAAGACAACAGCTTCTATGAAGCCGTTATCACTTACTACAACCCGGCAGAGGGTCGACACGCTTTGGTCTATGACATACATACACCAAATGAGACATGGGAATGGGTTGATCTTAAAGAGATCTCTCCTGAGGATATCTGTTGGGAAGATCCGGGCCCATCAGGACGCAGGGCTAAGAAATCTACAAGCCATACCTCAAGAAAGCGAGGACCCACAAAGTCTCAATCAAAGAAAGAACTTACATCGCAAAATGGTATAGGGAAGGCGCCAGATGATTTAGAATTATTAAATACTGATACCCTGGTGAAGGAGGTGGAGAGAGTTTTCAATGCAAGCGAGCCAGATCCTTTTGAGCTCGAAAAAGCAAGGAAAATTCTGAAAGATCATGAACAGGCCCTTGTTGATGCCATTCAAAGGCTGGCATATGCATCAGATGGTGAAAGTGATAGAGAGCACACATTTCTGCACAGTCGATCAATGGATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

376

Amino Acids

42.25

Weight (kDa)

5.72

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 51 - 119 9.7e-28 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 11
AbsI CCTCGAGG 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 904
AccB7I CCANNNNNTGG 1 cut(s) 485
AccI GTMKAC 3 cut(s) 137, 541, 694
AciI CCGC 2 cut(s) 553, 581
AclWI GGATC 2 cut(s) 782, 977
AcsI RAATTY 3 cut(s) 66, 156, 1011
AcuI CTGAAG 2 cut(s) 606, 660
AcyI GRCGYC 1 cut(s) 905
AfiI CCNNNNNNNGG 7 cut(s) 77, 485, 524, 688, 689, 946, 1053
AgsI TTSAA 5 cut(s) 410, 522, 578, 967, 1052
AjnI CCWGG 3 cut(s) 305, 484, 939
AluBI AGCT 4 cut(s) 298, 371, 651, 994
AluI AGCT 4 cut(s) 298, 371, 651, 994
Alw21I GWGCWC 2 cut(s) 996, 1095
Alw26I GTCTC 5 cut(s) 19, 91, 345, 725, 864
AlwI GGATC 2 cut(s) 782, 977
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 4 cut(s) 198, 635, 793, 1032
ApaI GGGCCC 1 cut(s) 797
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 3 cut(s) 66, 156, 1011
AspLEI GCGC 2 cut(s) 211, 907
AspS9I GGNCC 7 cut(s) 198, 527, 566, 793, 794, 848, 1033
AsuC2I CCSGG 2 cut(s) 683, 792
AsuHPI GGTGA 4 cut(s) 155, 541, 955, 1088
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 3 cut(s) 527, 566, 848
AxyI CCTNAGG 2 cut(s) 194, 768
BaeGI GKGCMC 1 cut(s) 797
BaeI ACNNNNGTAYC 2 cut(s) 927, 960
BanI GGYRCC 1 cut(s) 904
BanII GRGCYC 2 cut(s) 797, 996
BbsI GAAGAC 1 cut(s) 648
Bbv12I GWGCWC 2 cut(s) 996, 1095
BbvI GCAGC 1 cut(s) 358
BccI CCATC 4 cut(s) 58, 805, 1067, 1115
BceAI ACGGC 1 cut(s) 647
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 3 cut(s) 307, 486, 941
BcnI CCSGG 2 cut(s) 683, 792
BcoDI GTCTC 5 cut(s) 19, 91, 345, 725, 864
BfaI CTAG 3 cut(s) 129, 375, 420
BfmI CTRYAG 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 908
BglII AGATCT 2 cut(s) 101, 759
BisI GCNGC 2 cut(s) 372, 582
BlsI GCNGC 2 cut(s) 373, 583
Bme1390I CCNGG 5 cut(s) 307, 486, 683, 792, 941
Bme18I GGWCC 3 cut(s) 527, 566, 848
BmeT110I CYCGRG 1 cut(s) 72
BmgT120I GGNCC 7 cut(s) 198, 527, 566, 793, 794, 848, 1033
BmiI GGNNCC 4 cut(s) 568, 795, 850, 906
BmrFI CCNGG 5 cut(s) 307, 486, 683, 792, 941
BmsI GCATC 2 cut(s) 1033, 1076
BpiI GAAGAC 1 cut(s) 648
BpuEI CTTGAG 2 cut(s) 188, 820
BpuMI CCSGG 2 cut(s) 683, 792
Bsa29I ATCGAT 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 905
BsaJI CCNNGG 3 cut(s) 306, 484, 939
Bsc4I CCNNNNNNNGG 7 cut(s) 77, 485, 524, 688, 689, 946, 1053
Bse21I CCTNAGG 2 cut(s) 194, 768
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 3 cut(s) 307, 486, 941
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 3 cut(s) 306, 484, 939
BseGI GGATG 3 cut(s) 138, 286, 1126
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 7 cut(s) 77, 485, 524, 688, 689, 946, 1053
BseMII CTCAG 5 cut(s) 102, 260, 309, 404, 759
BseSI GKGCMC 1 cut(s) 797
BseXI GCAGC 1 cut(s) 358
BsgI GTGCAG 2 cut(s) 207, 1088
BshFI GGCC 4 cut(s) 200, 637, 795, 1034
BshNI GGYRCC 1 cut(s) 904
BshVI ATCGAT 1 cut(s) 330
BsiHKAI GWGCWC 2 cut(s) 996, 1095
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 2 cut(s) 683, 791
BslFI GGGAC 2 cut(s) 424, 552
BslI CCNNNNNNNGG 7 cut(s) 77, 485, 524, 688, 689, 946, 1053
BsmAI GTCTC 5 cut(s) 19, 91, 345, 725, 864
BsmFI GGGAC 2 cut(s) 424, 552
BsnI GGCC 4 cut(s) 200, 637, 795, 1034
BsoBI CYCGRG 1 cut(s) 72
Bsp120I GGGCCC 1 cut(s) 793
Bsp1286I GDGCHC 3 cut(s) 797, 996, 1095
Bsp143I GATC 8 cut(s) 40, 101, 748, 759, 787, 982, 1021, 1112
BspACI CCGC 2 cut(s) 553, 581
BspANI GGCC 4 cut(s) 200, 637, 795, 1034
BspCNI CTCAG 5 cut(s) 101, 261, 310, 403, 760
BspDI ATCGAT 1 cut(s) 330
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 4 cut(s) 568, 795, 850, 906
BspPI GGATC 2 cut(s) 782, 977
BspT107I GGYRCC 1 cut(s) 904
BssECI CCNNGG 3 cut(s) 306, 484, 939
BssMI GATC 8 cut(s) 40, 101, 748, 759, 787, 982, 1021, 1112
BssNAI GTATAC 1 cut(s) 138
BssNI GRCGYC 1 cut(s) 905
Bst1107I GTATAC 1 cut(s) 138
Bst2UI CCWGG 3 cut(s) 307, 486, 941
Bst4CI ACNGT 2 cut(s) 185, 1109
BstACI GRCGYC 1 cut(s) 905
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 3 cut(s) 973, 977, 1059
BstDEI CTNAG 8 cut(s) 88, 194, 257, 269, 318, 390, 768, 813
BstENI CCTNNNNNAGG 2 cut(s) 522, 944
BstF5I GGATG 3 cut(s) 138, 286, 1126
BstH2I RGCGCY 1 cut(s) 908
BstHHI GCGC 2 cut(s) 211, 907
BstKTI GATC 8 cut(s) 43, 104, 751, 762, 790, 985, 1024, 1115
BstMAI GTCTC 5 cut(s) 19, 91, 345, 725, 864
BstMBI GATC 8 cut(s) 40, 101, 748, 759, 787, 982, 1021, 1112
BstMWI GCNNNNNNNGC 4 cut(s) 185, 206, 475, 587
BstNI CCWGG 3 cut(s) 307, 486, 941
BstSCI CCNGG 5 cut(s) 305, 484, 681, 790, 939
BstSFI CTRYAG 1 cut(s) 108
BstSLI GKGCMC 1 cut(s) 797
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 648
BstX2I RGATCY 4 cut(s) 101, 759, 787, 982
BstYI RGATCY 4 cut(s) 101, 759, 787, 982
BstZ17I GTATAC 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 330
Bsu36I CCTNAGG 2 cut(s) 194, 768
BsuRI GGCC 4 cut(s) 200, 637, 795, 1034
BsuTUI ATCGAT 1 cut(s) 330
BtgZI GCGATG 1 cut(s) 867
BtsCI GGATG 3 cut(s) 138, 286, 1126
BtsIMutI CAGTG 2 cut(s) 30, 31
Cac8I GCNNGC 3 cut(s) 973, 977, 1059
CaiI CAGNNNCTG 1 cut(s) 530
CciI TCATGA 1 cut(s) 1024
CfoI GCGC 2 cut(s) 211, 907
Cfr13I GGNCC 7 cut(s) 198, 527, 566, 793, 794, 848, 1033
ClaI ATCGAT 1 cut(s) 330
CseI GACGC 2 cut(s) 426, 813
CviAII CATG 5 cut(s) 145, 470, 508, 735, 1025
DdeI CTNAG 8 cut(s) 88, 194, 257, 269, 318, 390, 768, 813
DinI GGCGCC 1 cut(s) 906
DpnI GATC 8 cut(s) 42, 103, 750, 761, 789, 984, 1023, 1114
DpnII GATC 8 cut(s) 40, 101, 748, 759, 787, 982, 1021, 1112
DraI TTTAAA 1 cut(s) 205
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
Ecl136II GAGCTC 1 cut(s) 994
Eco24I GRGCYC 2 cut(s) 797, 996
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 3 cut(s) 527, 566, 848
Eco53kI GAGCTC 1 cut(s) 994
Eco57I CTGAAG 2 cut(s) 606, 660
Eco81I CCTNAGG 2 cut(s) 194, 768
Eco88I CYCGRG 1 cut(s) 72
EcoICRI GAGCTC 1 cut(s) 994
EcoNI CCTNNNNNAGG 2 cut(s) 522, 944
EcoO109I RGGNCCY 4 cut(s) 198, 527, 848, 1033
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 3 cut(s) 305, 484, 939
EcoRV GATATC 1 cut(s) 775
EcoT22I ATGCAT 1 cut(s) 1069
EcoT38I GRGCYC 2 cut(s) 797, 996
EgeI GGCGCC 1 cut(s) 906
EheI GGCGCC 1 cut(s) 906
FaeI CATG 5 cut(s) 148, 473, 511, 738, 1028
FaqI GGGAC 2 cut(s) 424, 552
FatI CATG 5 cut(s) 144, 469, 507, 734, 1024
FauI CCCGC 1 cut(s) 560
FauNDI CATATG 1 cut(s) 1063
FblI GTMKAC 3 cut(s) 137, 541, 694
Fnu4HI GCNGC 2 cut(s) 372, 582
FokI GGATG 2 cut(s) 145, 293
FriOI GRGCYC 2 cut(s) 797, 996
Fsp4HI GCNGC 2 cut(s) 372, 582
FspBI CTAG 3 cut(s) 129, 375, 420
GlaI GCGC 2 cut(s) 210, 906
GluI GCNGC 2 cut(s) 372, 582
HaeII RGCGCY 1 cut(s) 908
HaeIII GGCC 4 cut(s) 200, 637, 795, 1034
HapII CCGG 2 cut(s) 683, 791
HgaI GACGC 2 cut(s) 426, 813
HhaI GCGC 2 cut(s) 211, 907
Hin1I GRCGYC 1 cut(s) 905
Hin1II CATG 5 cut(s) 148, 473, 511, 738, 1028
Hin6I GCGC 2 cut(s) 209, 905
HinP1I GCGC 2 cut(s) 209, 905
HincII GTYRAC 1 cut(s) 695
HindII GTYRAC 1 cut(s) 695
HinfI GANTC 1 cut(s) 602
HpaII CCGG 2 cut(s) 683, 791
HphI GGTGA 4 cut(s) 155, 541, 955, 1088
Hpy166II GTNNAC 4 cut(s) 138, 542, 627, 695
Hpy188I TCNGA 8 cut(s) 19, 280, 303, 319, 393, 601, 1017, 1072
Hpy188III TCNNGA 6 cut(s) 90, 441, 767, 801, 837, 1025
Hpy8I GTNNAC 4 cut(s) 138, 542, 627, 695
HpyAV CCTTC 6 cut(s) 229, 233, 528, 609, 895, 940
HpyCH4III ACNGT 2 cut(s) 185, 1109
HpyCH4V TGCA 4 cut(s) 188, 971, 1067, 1105
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 206, 475, 587
HpyF3I CTNAG 8 cut(s) 88, 194, 257, 269, 318, 390, 768, 813
Hsp92I GRCGYC 1 cut(s) 905
Hsp92II CATG 5 cut(s) 148, 473, 511, 738, 1028
HspAI GCGC 2 cut(s) 209, 905
KasI GGCGCC 1 cut(s) 904
Kzo9I GATC 8 cut(s) 40, 101, 748, 759, 787, 982, 1021, 1112
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 2 cut(s) 1033, 1076
MaeI CTAG 3 cut(s) 129, 375, 420
MalI GATC 8 cut(s) 42, 103, 750, 761, 789, 984, 1023, 1114
MboI GATC 8 cut(s) 40, 101, 748, 759, 787, 982, 1021, 1112
MboII GAAGA 4 cut(s) 35, 552, 653, 797
MflI RGATCY 4 cut(s) 101, 759, 787, 982
MhlI GDGCHC 3 cut(s) 797, 996, 1095
MluCI AATT 6 cut(s) 66, 156, 313, 545, 920, 1011
Mly113I GGCGCC 1 cut(s) 905
MmeI TCCRAC 2 cut(s) 514, 592
Mph1103I ATGCAT 1 cut(s) 1069
MseI TTAA 4 cut(s) 204, 312, 753, 926
MslI CAYNNNNRTG 1 cut(s) 739
MspI CCGG 2 cut(s) 683, 791
MspR9I CCNGG 5 cut(s) 307, 486, 683, 792, 941
MvaI CCWGG 3 cut(s) 307, 486, 941
MwoI GCNNNNNNNGC 4 cut(s) 185, 206, 475, 587
NarI GGCGCC 1 cut(s) 905
NciI CCSGG 2 cut(s) 683, 792
NdeI CATATG 1 cut(s) 1063
NdeII GATC 8 cut(s) 40, 101, 748, 759, 787, 982, 1021, 1112
NlaIII CATG 5 cut(s) 148, 473, 511, 738, 1028
NlaIV GGNNCC 4 cut(s) 568, 795, 850, 906
NsiI ATGCAT 1 cut(s) 1069
PaeR7I CTCGAG 1 cut(s) 72
PagI TCATGA 1 cut(s) 1024
PfeI GAWTC 1 cut(s) 602
PflMI CCANNNNNTGG 1 cut(s) 485
PkrI GCNGC 2 cut(s) 373, 583
PluTI GGCGCC 1 cut(s) 908
PpuMI RGGWCCY 2 cut(s) 527, 848
Psp124BI GAGCTC 1 cut(s) 996
Psp5II RGGWCCY 2 cut(s) 527, 848
Psp6I CCWGG 3 cut(s) 305, 484, 939
PspGI CCWGG 3 cut(s) 305, 484, 939
PspN4I GGNNCC 4 cut(s) 568, 795, 850, 906
PspOMI GGGCCC 1 cut(s) 793
PspPI GGNCC 7 cut(s) 198, 527, 566, 793, 794, 848, 1033
PspPPI RGGWCCY 2 cut(s) 527, 848
PspXI VCTCGAGB 1 cut(s) 72
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 4 cut(s) 101, 759, 787, 982
RseI CAYNNNNRTG 1 cut(s) 739
SacI GAGCTC 1 cut(s) 996
SalI GTCGAC 1 cut(s) 693
SaqAI TTAA 4 cut(s) 204, 312, 753, 926
SatI GCNGC 2 cut(s) 372, 582
Sau3AI GATC 8 cut(s) 40, 101, 748, 759, 787, 982, 1021, 1112
Sau96I GGNCC 7 cut(s) 198, 527, 566, 793, 794, 848, 1033
ScrFI CCNGG 5 cut(s) 307, 486, 683, 792, 941
SduI GDGCHC 3 cut(s) 797, 996, 1095
SfaNI GCATC 2 cut(s) 1033, 1076
SfcI CTRYAG 1 cut(s) 108
SfoI GGCGCC 1 cut(s) 906
Sfr274I CTCGAG 1 cut(s) 72
SinI GGWCC 3 cut(s) 527, 566, 848
SlaI CTCGAG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 739
SmlI CTYRAG 3 cut(s) 72, 167, 835
SmoI CTYRAG 3 cut(s) 72, 167, 835
Sse9I AATT 6 cut(s) 66, 156, 313, 545, 920, 1011
SsiI CCGC 2 cut(s) 553, 581
SspDI GGCGCC 1 cut(s) 904
SspMI CTAG 3 cut(s) 129, 375, 420
SstI GAGCTC 1 cut(s) 996
StyD4I CCNGG 5 cut(s) 305, 484, 681, 790, 939
TaaI ACNGT 2 cut(s) 185, 1109
TaqI TCGA 5 cut(s) 73, 330, 694, 996, 1111
TasI AATT 6 cut(s) 66, 156, 313, 545, 920, 1011
TauI GCSGC 1 cut(s) 584
TfiI GAWTC 1 cut(s) 602
Tru1I TTAA 4 cut(s) 204, 312, 753, 926
Tru9I TTAA 4 cut(s) 204, 312, 753, 926
TscAI CASTG 2 cut(s) 31, 37
TseI GCWGC 1 cut(s) 371
TspDTI ATGAA 8 cut(s) 149, 300, 375, 515, 524, 594, 672, 1041
TspRI CASTG 2 cut(s) 31, 37
Van91I CCANNNNNTGG 1 cut(s) 485
VpaK11BI GGWCC 3 cut(s) 527, 566, 848
XagI CCTNNNNNAGG 2 cut(s) 522, 944
XapI RAATTY 3 cut(s) 66, 156, 1011
XcmI CCANNNNNNNNNTGG 2 cut(s) 732, 1054
XhoI CTCGAG 1 cut(s) 72
XmiI GTMKAC 3 cut(s) 137, 541, 694
XspI CTAG 3 cut(s) 129, 375, 420
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.