Rh3DG256400

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001598
Physical Location & Seq
Reverse (-)
25329361 .. 25335169
5809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG256400.1

Sequence Viewer

Length: 1023 bp
ATGGAGGCCCAAATTCATCACCTAGAGCAAGAAGCATACTGTGCAGTCCTGCGTGCTTTCAAAGCCCAGTCTGATGCGATTACTTGGGAGAAGGAAGGTTTGATTACAGAACTTAGGAAAGAACTGAGAGTATCCGATGATGAACACAGAGAGCTTCTTGGCAGGGTTAACTCTGATGATATCATCAGTAAGATAAGAGAGTGGAGAACGGCAGGTGGGCATCAAGCTGCGAGATTCAGTGCATCCCAGCCTGTTTATGACCTTGTATCCAGTCCTACTGTTTCAGCATCCCGAAAGAAACAGAAAACATCCCAATCGCACCAGGGTCAGCCATTCCCTGGTTTATCCTCAATGAAGTCTATGCAGTACCCTTCTACAGGACCTTCTTTGAGTAGGCAGCTCACTAGCCGCAGTACTACTGGTCCCCTTGCCATAACTGAACCTGCCGAAGCAGCAACATATGATTTAATTGGAAAGAAAGTGTGGATGAGATGGCCTGCAGACAACAGCTTCTATGAAGCCCTTATAACAGACTACAACCCTAATGATGGTCGACATGCTTTGATCTACGATAAAAATACAGCAAATGAGACATTTGAATGGGTAGATTTCAAAGAGATATCTCGTGAGGATATCCGTTGGGAAGGTGAGGAACTGGGGCTATCTCATCAAGGGAACCATGGCAGTGGACAAGGACGTAGGGTTAAGAAATCTACGAACCATGGTGGTGTTGGTCCAGTTTCTGGGAGAGGTCGAGGACATATAAAGCCTCAATCAAAGAAACAACTTCTTCCTTCACAAAACGGCATAGGAAATAAGTTGCCAGATGATCTAGAATTATTGAATACTGATTCTCTGGTAAAGGAGGTGGAGAGAGTTTTCAATGCAAGTAATCCGGACCCCTTTGAGCTTGAAAAGGCAAGGAAAATGCTGAAAGATCACGAACAGGCACTTGTTGATGCCATTGAAAGGCTTGCATATGCATCCGATGGTGAAAGTGGTAATGGTTATATGTGTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

340

Amino Acids

37.96

Weight (kDa)

6.28

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 3 - 71 3.7e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
AarI CACCTGC 1 cut(s) 203
Acc36I ACCTGC 2 cut(s) 203, 451
AccB7I CCANNNNNTGG 2 cut(s) 338, 743
AccI GTMKAC 1 cut(s) 553
AccIII TCCGGA 1 cut(s) 895
AciI CCGC 1 cut(s) 409
AcsI RAATTY 1 cut(s) 12
AfaI GTAC 3 cut(s) 368, 415, 1018
AfiI CCNNNNNNNGG 5 cut(s) 338, 377, 548, 743, 969
AgsI TTSAA 7 cut(s) 61, 599, 613, 844, 883, 914, 968
AjnI CCWGG 2 cut(s) 321, 337
AluBI AGCT 5 cut(s) 154, 227, 400, 510, 910
AluI AGCT 5 cut(s) 154, 227, 400, 510, 910
Alw26I GTCTC 1 cut(s) 584
AlwNI CAGNNNCTG 1 cut(s) 743
Aor13HI TCCGGA 1 cut(s) 895
AoxI GGCC 2 cut(s) 6, 494
ApeKI GCWGC 3 cut(s) 227, 397, 452
ApoI RAATTY 1 cut(s) 12
AspS9I GGNCC 5 cut(s) 7, 380, 422, 734, 898
AsuHPI GGTGA 3 cut(s) 11, 659, 1004
AvaII GGWCC 4 cut(s) 380, 422, 734, 898
BauI CACGAG 1 cut(s) 624
BbvI GCAGC 3 cut(s) 214, 409, 464
BccI CCATC 3 cut(s) 486, 542, 983
BceAI ACGGC 2 cut(s) 225, 820
BciT130I CCWGG 2 cut(s) 323, 339
BciVI GTATCC 2 cut(s) 142, 277
BcoDI GTCTC 1 cut(s) 584
BfaI CTAG 3 cut(s) 23, 405, 833
BfmI CTRYAG 2 cut(s) 375, 498
BfuAI ACCTGC 2 cut(s) 203, 451
BfuI GTATCC 2 cut(s) 142, 277
BisI GCNGC 4 cut(s) 228, 398, 409, 453
BlsI GCNGC 4 cut(s) 229, 399, 410, 454
BmcAI AGTACT 1 cut(s) 415
Bme1390I CCNGG 2 cut(s) 323, 339
Bme18I GGWCC 4 cut(s) 380, 422, 734, 898
BmgT120I GGNCC 5 cut(s) 7, 380, 422, 734, 898
BmiI GGNNCC 3 cut(s) 424, 677, 900
BmrFI CCNGG 2 cut(s) 323, 339
BmrI ACTGGG 2 cut(s) 61, 665
BmsI GCATC 6 cut(s) 64, 229, 251, 296, 949, 992
BmuI ACTGGG 2 cut(s) 61, 665
BsaJI CCNNGG 4 cut(s) 322, 337, 679, 721
BsaWI WCCGGW 1 cut(s) 895
Bsc4I CCNNNNNNNGG 5 cut(s) 338, 377, 548, 743, 969
Bse1I ACTGG 5 cut(s) 67, 270, 424, 660, 737
BseAI TCCGGA 1 cut(s) 895
BseBI CCWGG 2 cut(s) 323, 339
BseDI CCNNGG 4 cut(s) 322, 337, 679, 721
BseGI GGATG 5 cut(s) 242, 287, 308, 492, 983
BseLI CCNNNNNNNGG 5 cut(s) 338, 377, 548, 743, 969
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 5 cut(s) 67, 270, 424, 660, 737
BseXI GCAGC 3 cut(s) 214, 409, 464
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 2 cut(s) 8, 496
BsiSI CCGG 1 cut(s) 896
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 5 cut(s) 338, 377, 548, 743, 969
BsmAI GTCTC 1 cut(s) 584
BsmFI GGGAC 1 cut(s) 408
BsnI GGCC 2 cut(s) 8, 496
Bsp13I TCCGGA 1 cut(s) 895
Bsp1407I TGTACA 1 cut(s) 1016
Bsp143I GATC 3 cut(s) 564, 829, 937
Bsp19I CCATGG 2 cut(s) 679, 721
BspACI CCGC 1 cut(s) 409
BspANI GGCC 2 cut(s) 8, 496
BspCNI CTCAG 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 895
BspLI GGNNCC 3 cut(s) 424, 677, 900
BspMAI CTGCAG 1 cut(s) 502
BspMI ACCTGC 2 cut(s) 203, 451
BsrGI TGTACA 1 cut(s) 1016
BsrI ACTGG 5 cut(s) 67, 270, 424, 660, 737
BssECI CCNNGG 4 cut(s) 322, 337, 679, 721
BssMI GATC 3 cut(s) 564, 829, 937
BssSI CACGAG 1 cut(s) 624
BssT1I CCWWGG 2 cut(s) 679, 721
Bst2BI CACGAG 1 cut(s) 624
Bst2UI CCWGG 2 cut(s) 323, 339
Bst4CI ACNGT 2 cut(s) 41, 280
BstAPI GCANNNNNTGC 1 cut(s) 41
BstAUI TGTACA 1 cut(s) 1016
BstC8I GCNNGC 3 cut(s) 54, 498, 975
BstDEI CTNAG 2 cut(s) 113, 125
BstDSI CCRYGG 2 cut(s) 679, 721
BstENI CCTNNNNNAGG 1 cut(s) 375
BstF5I GGATG 5 cut(s) 242, 287, 308, 492, 983
BstKTI GATC 3 cut(s) 567, 832, 940
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 3 cut(s) 564, 829, 937
BstMWI GCNNNNNNNGC 3 cut(s) 41, 62, 452
BstNI CCWGG 2 cut(s) 323, 339
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 2 cut(s) 321, 337
BstSFI CTRYAG 2 cut(s) 375, 498
BstV1I GCAGC 3 cut(s) 214, 409, 464
BstXI CCANNNNNNTGG 1 cut(s) 686
BsuI GTATCC 2 cut(s) 142, 277
BsuRI GGCC 2 cut(s) 8, 496
BtgI CCRYGG 2 cut(s) 679, 721
BtsCI GGATG 5 cut(s) 242, 287, 308, 492, 983
BtsI GCAGTG 1 cut(s) 691
BtsIMutI CAGTG 2 cut(s) 244, 691
BveI ACCTGC 2 cut(s) 203, 451
Cac8I GCNNGC 3 cut(s) 54, 498, 975
CaiI CAGNNNCTG 1 cut(s) 743
Cfr13I GGNCC 5 cut(s) 7, 380, 422, 734, 898
Csp6I GTAC 3 cut(s) 367, 414, 1017
CviAII CATG 3 cut(s) 557, 680, 722
CviQI GTAC 3 cut(s) 367, 414, 1017
DdeI CTNAG 2 cut(s) 113, 125
DpnI GATC 3 cut(s) 566, 831, 939
DpnII GATC 3 cut(s) 564, 829, 937
Eco130I CCWWGG 2 cut(s) 679, 721
Eco32I GATATC 3 cut(s) 181, 621, 634
Eco47I GGWCC 4 cut(s) 380, 422, 734, 898
EcoNI CCTNNNNNAGG 1 cut(s) 375
EcoO109I RGGNCCY 1 cut(s) 380
EcoRII CCWGG 2 cut(s) 321, 337
EcoRV GATATC 3 cut(s) 181, 621, 634
EcoT14I CCWWGG 2 cut(s) 679, 721
EcoT22I ATGCAT 1 cut(s) 985
ErhI CCWWGG 2 cut(s) 679, 721
FaeI CATG 3 cut(s) 560, 683, 725
FaqI GGGAC 1 cut(s) 408
FatI CATG 3 cut(s) 556, 679, 721
FauNDI CATATG 2 cut(s) 460, 979
FblI GTMKAC 1 cut(s) 553
Fnu4HI GCNGC 4 cut(s) 228, 398, 409, 453
FokI GGATG 5 cut(s) 229, 274, 295, 499, 970
Fsp4HI GCNGC 4 cut(s) 228, 398, 409, 453
FspBI CTAG 3 cut(s) 23, 405, 833
GluI GCNGC 4 cut(s) 228, 398, 409, 453
GsaI CCCAGC 1 cut(s) 250
HaeIII GGCC 2 cut(s) 8, 496
HapII CCGG 1 cut(s) 896
Hin1II CATG 3 cut(s) 560, 683, 725
HincII GTYRAC 2 cut(s) 169, 554
HindII GTYRAC 2 cut(s) 169, 554
HinfI GANTC 2 cut(s) 234, 851
HpaI GTTAAC 1 cut(s) 169
HpaII CCGG 1 cut(s) 896
HphI GGTGA 3 cut(s) 11, 659, 1004
Hpy166II GTNNAC 4 cut(s) 169, 554, 689, 1017
Hpy188I TCNGA 4 cut(s) 73, 136, 175, 988
Hpy188III TCNNGA 5 cut(s) 291, 626, 833, 896, 941
Hpy8I GTNNAC 4 cut(s) 169, 554, 689, 1017
HpyAV CCTTC 6 cut(s) 85, 89, 381, 393, 638, 804
HpyCH4III ACNGT 2 cut(s) 41, 280
HpyCH4IV ACGT 1 cut(s) 697
HpyCH4V TGCA 7 cut(s) 44, 242, 364, 500, 887, 977, 983
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 62, 452
HpyF3I CTNAG 2 cut(s) 113, 125
HpySE526I ACGT 1 cut(s) 697
Hsp92II CATG 3 cut(s) 560, 683, 725
Kpn2I TCCGGA 1 cut(s) 895
KspAI GTTAAC 1 cut(s) 169
Kzo9I GATC 3 cut(s) 564, 829, 937
Lsp1109I GCAGC 3 cut(s) 214, 409, 464
LweI GCATC 6 cut(s) 64, 229, 251, 296, 949, 992
MaeI CTAG 3 cut(s) 23, 405, 833
MaeII ACGT 1 cut(s) 697
MalI GATC 3 cut(s) 566, 831, 939
MboI GATC 3 cut(s) 564, 829, 937
MboII GAAGA 1 cut(s) 782
MluCI AATT 3 cut(s) 12, 468, 836
MnlI CCTC 7 cut(s) 358, 622, 643, 743, 749, 780, 859
Mph1103I ATGCAT 1 cut(s) 985
MroI TCCGGA 1 cut(s) 895
MseI TTAA 3 cut(s) 168, 467, 705
MslI CAYNNNNRTG 3 cut(s) 598, 684, 726
MspI CCGG 1 cut(s) 896
MspR9I CCNGG 2 cut(s) 323, 339
MvaI CCWGG 2 cut(s) 323, 339
MwoI GCNNNNNNNGC 3 cut(s) 41, 62, 452
NcoI CCATGG 2 cut(s) 679, 721
NdeI CATATG 2 cut(s) 460, 979
NdeII GATC 3 cut(s) 564, 829, 937
NlaIII CATG 3 cut(s) 560, 683, 725
NlaIV GGNNCC 3 cut(s) 424, 677, 900
NsiI ATGCAT 1 cut(s) 985
NspI RCATGY 1 cut(s) 560
PaqCI CACCTGC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 234, 851
PflMI CCANNNNNTGG 2 cut(s) 338, 743
PkrI GCNGC 4 cut(s) 229, 399, 410, 454
PpuMI RGGWCCY 1 cut(s) 380
PsiI TTATAA 1 cut(s) 527
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 2 cut(s) 321, 337
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 2 cut(s) 321, 337
PspN4I GGNNCC 3 cut(s) 424, 677, 900
PspPI GGNCC 5 cut(s) 7, 380, 422, 734, 898
PspPPI RGGWCCY 1 cut(s) 380
PstI CTGCAG 1 cut(s) 502
PstNI CAGNNNCTG 1 cut(s) 743
RsaI GTAC 3 cut(s) 368, 415, 1018
RsaNI GTAC 3 cut(s) 367, 414, 1017
RseI CAYNNNNRTG 3 cut(s) 598, 684, 726
SalI GTCGAC 1 cut(s) 552
SaqAI TTAA 3 cut(s) 168, 467, 705
SatI GCNGC 4 cut(s) 228, 398, 409, 453
Sau3AI GATC 3 cut(s) 564, 829, 937
Sau96I GGNCC 5 cut(s) 7, 380, 422, 734, 898
ScaI AGTACT 1 cut(s) 415
ScrFI CCNGG 2 cut(s) 323, 339
SfaNI GCATC 6 cut(s) 64, 229, 251, 296, 949, 992
SfcI CTRYAG 2 cut(s) 375, 498
SinI GGWCC 4 cut(s) 380, 422, 734, 898
SmiMI CAYNNNNRTG 3 cut(s) 598, 684, 726
Sse9I AATT 3 cut(s) 12, 468, 836
SsiI CCGC 1 cut(s) 409
SspMI CTAG 3 cut(s) 23, 405, 833
StyD4I CCNGG 2 cut(s) 321, 337
StyI CCWWGG 2 cut(s) 679, 721
TaaI ACNGT 2 cut(s) 41, 280
TaiI ACGT 1 cut(s) 700
TaqI TCGA 2 cut(s) 553, 754
TasI AATT 3 cut(s) 12, 468, 836
TatI WGTACW 2 cut(s) 413, 1016
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 2 cut(s) 234, 851
Tru1I TTAA 3 cut(s) 168, 467, 705
Tru9I TTAA 3 cut(s) 168, 467, 705
TscAI CASTG 2 cut(s) 244, 691
TseI GCWGC 3 cut(s) 227, 397, 452
TspDTI ATGAA 4 cut(s) 5, 156, 368, 531
TspGWI ACGGA 1 cut(s) 626
TspRI CASTG 2 cut(s) 244, 691
Van91I CCANNNNNTGG 2 cut(s) 338, 743
VpaK11BI GGWCC 4 cut(s) 380, 422, 734, 898
XagI CCTNNNNNAGG 1 cut(s) 375
XapI RAATTY 1 cut(s) 12
XbaI TCTAGA 1 cut(s) 832
XceI RCATGY 1 cut(s) 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 728
XmiI GTMKAC 1 cut(s) 553
XspI CTAG 3 cut(s) 23, 405, 833
ZrmI AGTACT 1 cut(s) 415
Zsp2I ATGCAT 1 cut(s) 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.