MD12G1058900.v1.1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001598
Physical Location & Seq
Forward (+)
6876878 .. 6881892
5015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1058900.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGGGTGACACAATTCTTGTACTATCAAGTATACCTTTATATGGAATAACTGCAGGCACTGATGATGATGACCTTCCTCCATCTCATCAAAACAGAATTCCTCAAGGGGGTCAAGTCTCAGGAAATAAAATATCTGCTGTAGATTCTATCTCATTTTCTAGGATGTATACAGACATGGAGGCACAAATTCATCACCTTGAGCAAGAAGCATACTGTGCGGTCCTACGGGCGTTTAAAGCGCAGTCAAATGCGATTACTTGGGAGAAGGAAGGTTTGATTACCGAACTTAGGAAAGAACTGAGAGTATCAGATGATGAACACAGAGAGCTTCTGACCAGGGTTAATTCTGAGGACATCATCGATAAGATAAGAGTGTGGAGAGACAAAGGTGGTCATCAAGCTGCCAGGCTTACTACATCTCAGAATGCATATGAACTTTTACCTAGTCCTACTATTTCAGCATCCCGAAAGAAACAAAAAACATCACAATCGCATGGTCAGCCATTCCCTGGTTTATCCTCAATGAAGTCCATGAAATACCCTTCAACAGGACCTGCTGGAAGTAGGCAATTCACCCGCAGTTCTTCTGGTCCCTTTGTCGAAGCAGCTGTTGCAGAAGCATCTGATTCATTAGTTGGAAGGAAAGTGTGGACAAGGTGGCCGGAAGACAACAGCTTCTATGAAGCCGTTATCACTTACTACAACCCGGCTGAGGGTCGACATGCTTTGGTCTATGACATACATACACCAAATGAGACGTGGGAATGGGTTGATCTCAAAGAGATCTCTCCTGAGGATATCTGTTGGGAAGATCCTGGCCCATCAGGACGCCGGGCTAAGAAATCTACTAGCCAGACCACAAGAAGGCGAGGACCCACAAAGTCTCAATCAAAGAAAGAACTTCCATCACAAAATGGTATAGGGAAGGTGTCAGATGATTTGGAATTATTAAATACTGACACCCTGGTAAAGGAGGTGGAGAGAGTTTTCAATGCAAGTGAGCCAGATCCCTTTGAGCTCGAAAAAGCAAGGAAAATGCTGAAAGATCACGAACAGGCCCTTGTTGACGCCATTCAAAGGCTGGCATATGCATCGGATGGTGAAAGTGATGGAGAGCACACGTTTCTGCACAGTCGGTCAATGGATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT

Protein Analysis

384

Amino Acids

42.83

Weight (kDa)

5.65

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 61 - 129 4.7e-28 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 562
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 3 cut(s) 31, 167, 718
AciI CCGC 2 cut(s) 218, 577
AclWI GGATC 2 cut(s) 806, 1001
AcoI YGGCCR 1 cut(s) 659
AcsI RAATTY 2 cut(s) 96, 186
AcyI GRCGYC 2 cut(s) 829, 1068
AfaI GTAC 1 cut(s) 21
AflIII ACRYGT 1 cut(s) 1119
AgsI TTSAA 3 cut(s) 546, 991, 1076
AjiI CACGTC 1 cut(s) 759
AjnI CCWGG 5 cut(s) 335, 404, 508, 814, 963
AluBI AGCT 5 cut(s) 328, 401, 608, 675, 1018
AluI AGCT 5 cut(s) 328, 401, 608, 675, 1018
Alw21I GWGCWC 2 cut(s) 1020, 1119
Alw26I GTCTC 4 cut(s) 121, 375, 749, 888
AlwI GGATC 2 cut(s) 806, 1001
AlwNI CAGNNNCTG 2 cut(s) 59, 554
AoxI GGCC 3 cut(s) 659, 817, 1056
ApeKI GCWGC 2 cut(s) 401, 605
ApoI RAATTY 2 cut(s) 96, 186
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 6 cut(s) 220, 551, 590, 818, 872, 1057
AsuC2I CCSGG 2 cut(s) 707, 833
AsuHPI GGTGA 4 cut(s) 17, 185, 565, 1112
AvaII GGWCC 4 cut(s) 220, 551, 590, 872
AxyI CCTNAGG 1 cut(s) 792
BanII GRGCYC 1 cut(s) 1020
BbsI GAAGAC 1 cut(s) 672
Bbv12I GWGCWC 2 cut(s) 1020, 1119
BbvCI CCTCAGC 1 cut(s) 711
BbvI GCAGC 2 cut(s) 388, 617
BccI CCATC 6 cut(s) 88, 829, 913, 1091, 1103, 1139
BceAI ACGGC 1 cut(s) 671
BcgI CGANNNNNNTGC 2 cut(s) 1074, 1108
BciT130I CCWGG 5 cut(s) 337, 406, 510, 816, 965
BcnI CCSGG 2 cut(s) 707, 833
BcoDI GTCTC 4 cut(s) 121, 375, 749, 888
BfaI CTAG 3 cut(s) 159, 444, 849
BfmI CTRYAG 2 cut(s) 51, 138
BfuAI ACCTGC 1 cut(s) 562
BglII AGATCT 1 cut(s) 783
BisI GCNGC 2 cut(s) 402, 606
BlsI GCNGC 2 cut(s) 403, 607
Bme1390I CCNGG 7 cut(s) 337, 406, 510, 707, 816, 833, 965
Bme18I GGWCC 4 cut(s) 220, 551, 590, 872
BmgBI CACGTC 1 cut(s) 759
BmgT120I GGNCC 6 cut(s) 220, 551, 590, 818, 872, 1057
BmiI GGNNCC 2 cut(s) 592, 874
BmrFI CCNGG 7 cut(s) 337, 406, 510, 707, 816, 833, 965
BmsI GCATC 3 cut(s) 470, 629, 1100
BpiI GAAGAC 1 cut(s) 672
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 2 cut(s) 87, 218
BpuMI CCSGG 2 cut(s) 707, 833
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 2 cut(s) 829, 1068
BsaJI CCNNGG 3 cut(s) 336, 508, 963
Bse21I CCTNAGG 1 cut(s) 792
BseBI CCWGG 5 cut(s) 337, 406, 510, 816, 965
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 3 cut(s) 336, 508, 963
BseGI GGATG 4 cut(s) 168, 461, 1102, 1150
BseMII CTCAG 6 cut(s) 132, 290, 339, 434, 702, 783
BseXI GCAGC 2 cut(s) 388, 617
BsgI GTGCAG 1 cut(s) 1112
BshFI GGCC 3 cut(s) 661, 819, 1058
BshVI ATCGAT 1 cut(s) 360
BsiHKAI GWGCWC 2 cut(s) 1020, 1119
BsiSI CCGG 3 cut(s) 662, 707, 832
BslFI GGGAC 1 cut(s) 576
BsmAI GTCTC 4 cut(s) 121, 375, 749, 888
BsmBI CGTCTC 1 cut(s) 749
BsmFI GGGAC 1 cut(s) 576
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 3 cut(s) 661, 819, 1058
Bsp1286I GDGCHC 2 cut(s) 1020, 1119
Bsp143I GATC 5 cut(s) 772, 783, 811, 1006, 1045
BspACI CCGC 2 cut(s) 218, 577
BspANI GGCC 3 cut(s) 661, 819, 1058
BspCNI CTCAG 6 cut(s) 131, 291, 340, 433, 703, 784
BspDI ATCGAT 1 cut(s) 360
BspLI GGNNCC 2 cut(s) 592, 874
BspMAI CTGCAG 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 2 cut(s) 806, 1001
BssECI CCNNGG 3 cut(s) 336, 508, 963
BssMI GATC 5 cut(s) 772, 783, 811, 1006, 1045
BssNAI GTATAC 2 cut(s) 32, 168
BssNI GRCGYC 2 cut(s) 829, 1068
Bst1107I GTATAC 2 cut(s) 32, 168
Bst2UI CCWGG 5 cut(s) 337, 406, 510, 816, 965
Bst4CI ACNGT 2 cut(s) 215, 1133
BstACI GRCGYC 2 cut(s) 829, 1068
BstAPI GCANNNNNTGC 2 cut(s) 215, 611
BstC8I GCNNGC 2 cut(s) 55, 1083
BstDEI CTNAG 8 cut(s) 118, 287, 299, 348, 420, 711, 792, 837
BstENI CCTNNNNNAGG 2 cut(s) 546, 968
BstF5I GGATG 4 cut(s) 168, 461, 1102, 1150
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 5 cut(s) 775, 786, 814, 1009, 1048
BstMAI GTCTC 4 cut(s) 121, 375, 749, 888
BstMBI GATC 5 cut(s) 772, 783, 811, 1006, 1045
BstMWI GCNNNNNNNGC 4 cut(s) 215, 236, 499, 611
BstNI CCWGG 5 cut(s) 337, 406, 510, 816, 965
BstNSI RCATGY 1 cut(s) 725
BstSCI CCNGG 7 cut(s) 335, 404, 508, 705, 814, 831, 963
BstSFI CTRYAG 2 cut(s) 51, 138
BstV1I GCAGC 2 cut(s) 388, 617
BstV2I GAAGAC 1 cut(s) 672
BstX2I RGATCY 3 cut(s) 783, 811, 1006
BstYI RGATCY 3 cut(s) 783, 811, 1006
BstZ17I GTATAC 2 cut(s) 32, 168
Bsu15I ATCGAT 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 3 cut(s) 661, 819, 1058
BsuTUI ATCGAT 1 cut(s) 360
BtrI CACGTC 1 cut(s) 759
BtsCI GGATG 4 cut(s) 168, 461, 1102, 1150
BtsIMutI CAGTG 1 cut(s) 57
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 2 cut(s) 55, 1083
CaiI CAGNNNCTG 2 cut(s) 59, 554
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 6 cut(s) 220, 551, 590, 818, 872, 1057
ClaI ATCGAT 1 cut(s) 360
CseI GACGC 2 cut(s) 837, 1076
Csp6I GTAC 1 cut(s) 20
CviAII CATG 4 cut(s) 175, 494, 532, 722
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 8 cut(s) 118, 287, 299, 348, 420, 711, 792, 837
DpnI GATC 5 cut(s) 774, 785, 813, 1008, 1047
DpnII GATC 5 cut(s) 772, 783, 811, 1006, 1045
DraI TTTAAA 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 659
Ecl136II GAGCTC 1 cut(s) 1018
Eco24I GRGCYC 1 cut(s) 1020
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 4 cut(s) 220, 551, 590, 872
Eco53kI GAGCTC 1 cut(s) 1018
Eco81I CCTNAGG 1 cut(s) 792
EcoICRI GAGCTC 1 cut(s) 1018
EcoNI CCTNNNNNAGG 2 cut(s) 546, 968
EcoO109I RGGNCCY 3 cut(s) 551, 872, 1057
EcoRI GAATTC 1 cut(s) 96
EcoRII CCWGG 5 cut(s) 335, 404, 508, 814, 963
EcoRV GATATC 1 cut(s) 799
EcoT22I ATGCAT 2 cut(s) 430, 1093
EcoT38I GRGCYC 1 cut(s) 1020
Esp3I CGTCTC 1 cut(s) 749
FaeI CATG 4 cut(s) 178, 497, 535, 725
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FaqI GGGAC 1 cut(s) 576
FatI CATG 4 cut(s) 174, 493, 531, 721
FauI CCCGC 1 cut(s) 584
FauNDI CATATG 2 cut(s) 430, 1087
FblI GTMKAC 3 cut(s) 31, 167, 718
Fnu4HI GCNGC 2 cut(s) 402, 606
FokI GGATG 3 cut(s) 175, 448, 1109
FriOI GRGCYC 1 cut(s) 1020
Fsp4HI GCNGC 2 cut(s) 402, 606
FspBI CTAG 3 cut(s) 159, 444, 849
GlaI GCGC 1 cut(s) 240
GluI GCNGC 2 cut(s) 402, 606
HaeIII GGCC 3 cut(s) 661, 819, 1058
HapII CCGG 3 cut(s) 662, 707, 832
HgaI GACGC 2 cut(s) 837, 1076
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 2 cut(s) 829, 1068
Hin1II CATG 4 cut(s) 178, 497, 535, 725
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 2 cut(s) 719, 1066
HindII GTYRAC 2 cut(s) 719, 1066
HinfI GANTC 2 cut(s) 143, 626
HpaII CCGG 3 cut(s) 662, 707, 832
HphI GGTGA 4 cut(s) 17, 185, 565, 1112
Hpy166II GTNNAC 5 cut(s) 32, 168, 651, 719, 1066
Hpy188I TCNGA 7 cut(s) 310, 333, 349, 423, 625, 934, 1096
Hpy188III TCNNGA 5 cut(s) 120, 465, 791, 825, 1049
Hpy8I GTNNAC 5 cut(s) 32, 168, 651, 719, 1066
HpyAV CCTTC 7 cut(s) 83, 259, 263, 552, 633, 858, 919
HpyCH4III ACNGT 2 cut(s) 215, 1133
HpyCH4IV ACGT 2 cut(s) 758, 1121
HpyCH4V TGCA 6 cut(s) 53, 428, 614, 995, 1091, 1129
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 236, 499, 611
HpyF3I CTNAG 8 cut(s) 118, 287, 299, 348, 420, 711, 792, 837
HpySE526I ACGT 2 cut(s) 758, 1121
Hsp92I GRCGYC 2 cut(s) 829, 1068
Hsp92II CATG 4 cut(s) 178, 497, 535, 725
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 5 cut(s) 772, 783, 811, 1006, 1045
Lsp1109I GCAGC 2 cut(s) 388, 617
LweI GCATC 3 cut(s) 470, 629, 1100
MaeI CTAG 3 cut(s) 159, 444, 849
MaeII ACGT 2 cut(s) 758, 1121
MaeIII GTNAC 1 cut(s) 5
MalI GATC 5 cut(s) 774, 785, 813, 1008, 1047
MboI GATC 5 cut(s) 772, 783, 811, 1006, 1045
MboII GAAGA 3 cut(s) 576, 677, 821
MflI RGATCY 3 cut(s) 783, 811, 1006
MhlI GDGCHC 2 cut(s) 1020, 1119
MluCI AATT 6 cut(s) 12, 96, 186, 343, 569, 944
MmeI TCCRAC 1 cut(s) 616
MnlI CCTC 9 cut(s) 87, 111, 172, 343, 529, 706, 787, 863, 967
Mph1103I ATGCAT 2 cut(s) 430, 1093
MseI TTAA 3 cut(s) 234, 342, 950
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 3 cut(s) 662, 707, 832
MspR9I CCNGG 7 cut(s) 337, 406, 510, 707, 816, 833, 965
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 5 cut(s) 337, 406, 510, 816, 965
MwoI GCNNNNNNNGC 4 cut(s) 215, 236, 499, 611
NciI CCSGG 2 cut(s) 707, 833
NdeI CATATG 2 cut(s) 430, 1087
NdeII GATC 5 cut(s) 772, 783, 811, 1006, 1045
NlaIII CATG 4 cut(s) 178, 497, 535, 725
NlaIV GGNNCC 2 cut(s) 592, 874
NmuCI GTSAC 1 cut(s) 5
NsiI ATGCAT 2 cut(s) 430, 1093
NspI RCATGY 1 cut(s) 725
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 2 cut(s) 143, 626
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 2 cut(s) 403, 607
PpuMI RGGWCCY 2 cut(s) 551, 872
Psp124BI GAGCTC 1 cut(s) 1020
Psp5II RGGWCCY 2 cut(s) 551, 872
Psp6I CCWGG 5 cut(s) 335, 404, 508, 814, 963
PspGI CCWGG 5 cut(s) 335, 404, 508, 814, 963
PspN4I GGNNCC 2 cut(s) 592, 874
PspPI GGNCC 6 cut(s) 220, 551, 590, 818, 872, 1057
PspPPI RGGWCCY 2 cut(s) 551, 872
PstI CTGCAG 1 cut(s) 55
PstNI CAGNNNCTG 2 cut(s) 59, 554
PsuI RGATCY 3 cut(s) 783, 811, 1006
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
SacI GAGCTC 1 cut(s) 1020
SalI GTCGAC 1 cut(s) 717
SaqAI TTAA 3 cut(s) 234, 342, 950
SatI GCNGC 2 cut(s) 402, 606
Sau3AI GATC 5 cut(s) 772, 783, 811, 1006, 1045
Sau96I GGNCC 6 cut(s) 220, 551, 590, 818, 872, 1057
ScrFI CCNGG 7 cut(s) 337, 406, 510, 707, 816, 833, 965
SduI GDGCHC 2 cut(s) 1020, 1119
SfaNI GCATC 3 cut(s) 470, 629, 1100
SfcI CTRYAG 2 cut(s) 51, 138
SinI GGWCC 4 cut(s) 220, 551, 590, 872
SmlI CTYRAG 2 cut(s) 102, 197
SmoI CTYRAG 2 cut(s) 102, 197
Sse9I AATT 6 cut(s) 12, 96, 186, 343, 569, 944
SsiI CCGC 2 cut(s) 218, 577
SspMI CTAG 3 cut(s) 159, 444, 849
SstI GAGCTC 1 cut(s) 1020
StyD4I CCNGG 7 cut(s) 335, 404, 508, 705, 814, 831, 963
TaaI ACNGT 2 cut(s) 215, 1133
TaiI ACGT 2 cut(s) 761, 1124
TaqI TCGA 4 cut(s) 360, 600, 718, 1020
TaqII GACCGA 1 cut(s) 1125
TasI AATT 6 cut(s) 12, 96, 186, 343, 569, 944
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 2 cut(s) 143, 626
Tru1I TTAA 3 cut(s) 234, 342, 950
Tru9I TTAA 3 cut(s) 234, 342, 950
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 2 cut(s) 401, 605
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 7 cut(s) 179, 330, 447, 539, 548, 618, 696
TspRI CASTG 1 cut(s) 64
Van91I CCANNNNNTGG 1 cut(s) 509
VpaK11BI GGWCC 4 cut(s) 220, 551, 590, 872
XagI CCTNNNNNAGG 2 cut(s) 546, 968
XapI RAATTY 2 cut(s) 96, 186
XceI RCATGY 1 cut(s) 725
XcmI CCANNNNNNNNNTGG 2 cut(s) 756, 1078
XmiI GTMKAC 3 cut(s) 31, 167, 718
XspI CTAG 3 cut(s) 159, 444, 849
Zsp2I ATGCAT 2 cut(s) 430, 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.