Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0001598

Physical Location & Seq

Genomic Coordinates Reverse (-)

4493146 .. 4497540

Gene Structure 4395 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom14g04800.6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGGACTATGATGTCTCCGAAATCAGTGGCACTGATGATGATCTTCCTCCATCTCATCAAAACAGAATTCCTCGAGGGGGTAAAGTCTCGGGAAACGAAAGATCTGCTGTAGGTTCTATCTCATTTTCTAGGATGTATACAGACATGGAGGCTGAAATTCATCACCTTGAGCAAGAAGCATACTGTGCAGTCCTAAGGGCGTTTAAAGCGCAGTCTAATGCGATTACTTGGGAGAAGGAAGGTTTGATTACTGAACTTAGGAAAGAACTGAGAGTATCGGATGATGAACACAGAGAGCTTCTGACCATGGTTAATTCTGAGGACATCATCGATAAGATAAGAGTGTGGAGAGACAAAGGTGGTCATCAAGCTGCTAGGCTTACTTCATCTCAGAATGTATATGAACTTTTACCTAGTCCTAGTATTTCAGCGTCCCGAAAGAAACAGAAAACATCACAATCGCATGGTCAGCCATTCCCTGGTTTATCCTCAATGAAGTCCATGAAATACCCTTCAACAGGACCTGTCGGAAGTAGACAATTCACCCGCAGTTCTTCTGGTCCCTTTGTTGAAGCGGCTGAAGCAGAAACGTCTGATTCATTAGTTGGAAGGAAAGTGTGGACAAGGTGGCCTGAAGACAACAGCTTCTATGAAGCCGTTATCACTTACTACAACCCGGCAGAGGGTCGACACGCTTTGGTCTACGACATACATACACCAAATGAGACATGGGAATGGGTTGATCTTAAAGAGATCTCTCCTGAGGATATCTGTTGGGAAGATCCGGGCCCATCAGGACGCAGGGCTAAGAAATCTACGAGCCATACCACAAGAAAGCGAGGACCCACAAAGTCTCAATCAAAGAAAGAACTTCCGTCGCAAAATGGTATAGGGAAGGCGCCAGATGATTTAGAATTATTAAATACCGATACCCTGGTGAAGGAGGTGGAGAGAGTTTTCAATGCAAGTGAGCCAGATCCTTTTGAGCTCGAAAAAGCAAGGAAAATTCTGAAAGATCACGAACAGGCCCTTGTTGATGCCATTCAAAGGCTGGCATATGCATCGGATGGTGAAAGTGATAGAGAGCACACATTTCTGCACAGTCGATCAATGGATGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ENT

Protein Analysis

374

Amino Acids

42.03

Weight (kDa)

5.64

Isoelectric Point (pI)

46.24

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001598)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12140 AT3G12140 AT3G12140 AT5G06780 AT5G06780
fragaria_vesca	FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490 FvH4_6g22490
malus_domestica	MD12G1058900.v1.1 MD14G1059100.v1.1
prunus_persica	Prupe.7G075700_v2.0.a1 Prupe.7G075700_v2.0.a1 Prupe.7G075700_v2.0.a1 Prupe.7G075700_v2.0.a1 Prupe.7G075700_v2.0.a1 Prupe.7G075700_v2.0.a1 Prupe.7G075700_v2.0.a1 Prupe.7G075700_v2.0.a1 Prupe.7G075700_v2.0.a1
pyrus_communis	pycom12g05200 pycom14g04800
rosa_chinensis	RchiOBHm_Chr3g0479901
rosa_laevigata	RLG00000023526
rosa_multiflora	Rmu_sc0003400.1_g000027
rosa_roxburghii	Rroxscaffold_6G00401910
rosa_rugosa	Rorug03G0179300 Rorug03G0179300 Rorug03G0179400 Rorug03G0179500
rosa_samantha	Rh3AG230400 Rh3BG263000 Rh3CG260600 Rh3DG256400
rosa_wichuraiana	Rw0G015230 Rw3G020750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	11
AbsI	CCTCGAGG	1 cut(s)	72
AccB1I	GGYRCC	1 cut(s)	898
AccB7I	CCANNNNNTGG	1 cut(s)	479
AccI	GTMKAC	4 cut(s)	137, 535, 688, 702
AciI	CCGC	2 cut(s)	547, 575
AclWI	GGATC	2 cut(s)	776, 971
AcsI	RAATTY	3 cut(s)	66, 156, 1005
AcuI	CTGAAG	2 cut(s)	600, 654
AcyI	GRCGYC	1 cut(s)	899
AfiI	CCNNNNNNNGG	7 cut(s)	77, 479, 518, 682, 683, 940, 1047
AgsI	TTSAA	4 cut(s)	516, 572, 961, 1046
AjnI	CCWGG	2 cut(s)	478, 933
AluBI	AGCT	4 cut(s)	298, 371, 645, 988
AluI	AGCT	4 cut(s)	298, 371, 645, 988
Alw21I	GWGCWC	2 cut(s)	990, 1089
Alw26I	GTCTC	5 cut(s)	19, 91, 345, 719, 858
AlwI	GGATC	2 cut(s)	776, 971
AlwNI	CAGNNNCTG	1 cut(s)	524
Ama87I	CYCGRG	2 cut(s)	72, 88
AoxI	GGCC	3 cut(s)	629, 787, 1026
ApaI	GGGCCC	1 cut(s)	791
ApeKI	GCWGC	1 cut(s)	371
ApoI	RAATTY	3 cut(s)	66, 156, 1005
AspLEI	GCGC	2 cut(s)	211, 901
AspS9I	GGNCC	6 cut(s)	521, 560, 787, 788, 842, 1027
AsuC2I	CCSGG	2 cut(s)	677, 786
AsuHPI	GGTGA	4 cut(s)	155, 535, 949, 1082
AvaI	CYCGRG	2 cut(s)	72, 88
AvaII	GGWCC	3 cut(s)	521, 560, 842
AxyI	CCTNAGG	2 cut(s)	194, 762
BaeGI	GKGCMC	1 cut(s)	791
BaeI	ACNNNNGTAYC	2 cut(s)	921, 954
BanI	GGYRCC	1 cut(s)	898
BanII	GRGCYC	2 cut(s)	791, 990
BbsI	GAAGAC	1 cut(s)	642
Bbv12I	GWGCWC	2 cut(s)	990, 1089
BbvI	GCAGC	1 cut(s)	358
BccI	CCATC	4 cut(s)	58, 799, 1061, 1109
BceAI	ACGGC	1 cut(s)	641
BcgI	CGANNNNNNTGC	4 cut(s)	86, 120, 1044, 1078
BciT130I	CCWGG	2 cut(s)	480, 935
BcnI	CCSGG	2 cut(s)	677, 786
BcoDI	GTCTC	5 cut(s)	19, 91, 345, 719, 858
BfaI	CTAG	4 cut(s)	129, 375, 414, 420
BfmI	CTRYAG	1 cut(s)	108
BfoI	RGCGCY	1 cut(s)	902
BglII	AGATCT	2 cut(s)	101, 753
BisI	GCNGC	2 cut(s)	372, 576
BlsI	GCNGC	2 cut(s)	373, 577
Bme1390I	CCNGG	4 cut(s)	480, 677, 786, 935
Bme18I	GGWCC	3 cut(s)	521, 560, 842
BmeT110I	CYCGRG	2 cut(s)	72, 88
BmgT120I	GGNCC	6 cut(s)	521, 560, 787, 788, 842, 1027
BmiI	GGNNCC	4 cut(s)	562, 789, 844, 900
BmrFI	CCNGG	4 cut(s)	480, 677, 786, 935
BmsI	GCATC	2 cut(s)	1027, 1070
BpiI	GAAGAC	1 cut(s)	642
BpuEI	CTTGAG	1 cut(s)	188
BpuMI	CCSGG	2 cut(s)	677, 786
Bsa29I	ATCGAT	1 cut(s)	330
BsaBI	GATNNNNATC	1 cut(s)	39
BsaHI	GRCGYC	1 cut(s)	899
BsaJI	CCNNGG	3 cut(s)	306, 478, 933
Bsc4I	CCNNNNNNNGG	7 cut(s)	77, 479, 518, 682, 683, 940, 1047
Bse21I	CCTNAGG	2 cut(s)	194, 762
Bse8I	GATNNNNATC	1 cut(s)	39
BseBI	CCWGG	2 cut(s)	480, 935
BseCI	ATCGAT	1 cut(s)	330
BseDI	CCNNGG	3 cut(s)	306, 478, 933
BseGI	GGATG	4 cut(s)	138, 286, 1072, 1120
BseJI	GATNNNNATC	1 cut(s)	39
BseLI	CCNNNNNNNGG	7 cut(s)	77, 479, 518, 682, 683, 940, 1047
BseMII	CTCAG	4 cut(s)	260, 309, 404, 753
BseSI	GKGCMC	1 cut(s)	791
BseXI	GCAGC	1 cut(s)	358
BsgI	GTGCAG	2 cut(s)	207, 1082
BshFI	GGCC	3 cut(s)	631, 789, 1028
BshNI	GGYRCC	1 cut(s)	898
BshVI	ATCGAT	1 cut(s)	330
BsiHKAI	GWGCWC	2 cut(s)	990, 1089
BsiHKCI	CYCGRG	2 cut(s)	72, 88
BsiSI	CCGG	2 cut(s)	677, 785
BslFI	GGGAC	2 cut(s)	418, 546
BslI	CCNNNNNNNGG	7 cut(s)	77, 479, 518, 682, 683, 940, 1047
BsmAI	GTCTC	5 cut(s)	19, 91, 345, 719, 858
BsmFI	GGGAC	2 cut(s)	418, 546
BsnI	GGCC	3 cut(s)	631, 789, 1028
BsoBI	CYCGRG	2 cut(s)	72, 88
Bsp120I	GGGCCC	1 cut(s)	787
Bsp1286I	GDGCHC	3 cut(s)	791, 990, 1089
Bsp143I	GATC	8 cut(s)	40, 101, 742, 753, 781, 976, 1015, 1106
Bsp19I	CCATGG	1 cut(s)	306
BspACI	CCGC	2 cut(s)	547, 575
BspANI	GGCC	3 cut(s)	631, 789, 1028
BspCNI	CTCAG	4 cut(s)	261, 310, 403, 754
BspDI	ATCGAT	1 cut(s)	330
BspLI	GGNNCC	4 cut(s)	562, 789, 844, 900
BspPI	GGATC	2 cut(s)	776, 971
BspT107I	GGYRCC	1 cut(s)	898
BssECI	CCNNGG	3 cut(s)	306, 478, 933
BssMI	GATC	8 cut(s)	40, 101, 742, 753, 781, 976, 1015, 1106
BssNAI	GTATAC	1 cut(s)	138
BssNI	GRCGYC	1 cut(s)	899
BssT1I	CCWWGG	1 cut(s)	306
Bst1107I	GTATAC	1 cut(s)	138
Bst2UI	CCWGG	2 cut(s)	480, 935
Bst4CI	ACNGT	2 cut(s)	185, 1103
BstACI	GRCGYC	1 cut(s)	899
BstAPI	GCANNNNNTGC	1 cut(s)	185
BstC8I	GCNNGC	1 cut(s)	1053
BstDEI	CTNAG	7 cut(s)	194, 257, 269, 318, 390, 762, 807
BstDSI	CCRYGG	1 cut(s)	306
BstENI	CCTNNNNNAGG	2 cut(s)	516, 938
BstF5I	GGATG	4 cut(s)	138, 286, 1072, 1120
BstH2I	RGCGCY	1 cut(s)	902
BstHHI	GCGC	2 cut(s)	211, 901
BstKTI	GATC	8 cut(s)	43, 104, 745, 756, 784, 979, 1018, 1109
BstMAI	GTCTC	5 cut(s)	19, 91, 345, 719, 858
BstMBI	GATC	8 cut(s)	40, 101, 742, 753, 781, 976, 1015, 1106
BstMWI	GCNNNNNNNGC	4 cut(s)	185, 206, 469, 581
BstNI	CCWGG	2 cut(s)	480, 935
BstSCI	CCNGG	4 cut(s)	478, 675, 784, 933
BstSFI	CTRYAG	1 cut(s)	108
BstSLI	GKGCMC	1 cut(s)	791
BstV1I	GCAGC	1 cut(s)	358
BstV2I	GAAGAC	1 cut(s)	642
BstX2I	RGATCY	4 cut(s)	101, 753, 781, 976
BstYI	RGATCY	4 cut(s)	101, 753, 781, 976
BstZ17I	GTATAC	1 cut(s)	138
Bsu15I	ATCGAT	1 cut(s)	330
Bsu36I	CCTNAGG	2 cut(s)	194, 762
BsuRI	GGCC	3 cut(s)	631, 789, 1028
BsuTUI	ATCGAT	1 cut(s)	330
BtgI	CCRYGG	1 cut(s)	306
BtsCI	GGATG	4 cut(s)	138, 286, 1072, 1120
BtsIMutI	CAGTG	2 cut(s)	30, 31
Cac8I	GCNNGC	1 cut(s)	1053
CaiI	CAGNNNCTG	1 cut(s)	524
CfoI	GCGC	2 cut(s)	211, 901
Cfr13I	GGNCC	6 cut(s)	521, 560, 787, 788, 842, 1027
ClaI	ATCGAT	1 cut(s)	330
CseI	GACGC	2 cut(s)	420, 807
CviAII	CATG	5 cut(s)	145, 307, 464, 502, 729
DdeI	CTNAG	7 cut(s)	194, 257, 269, 318, 390, 762, 807
DinI	GGCGCC	1 cut(s)	900
DpnI	GATC	8 cut(s)	42, 103, 744, 755, 783, 978, 1017, 1108
DpnII	GATC	8 cut(s)	40, 101, 742, 753, 781, 976, 1015, 1106
DraI	TTTAAA	1 cut(s)	205
DrdI	GACNNNNNNGTC	1 cut(s)	11
DseDI	GACNNNNNNGTC	1 cut(s)	11
Ecl136II	GAGCTC	1 cut(s)	988
Eco130I	CCWWGG	1 cut(s)	306
Eco24I	GRGCYC	2 cut(s)	791, 990
Eco32I	GATATC	1 cut(s)	769
Eco47I	GGWCC	3 cut(s)	521, 560, 842
Eco53kI	GAGCTC	1 cut(s)	988
Eco57I	CTGAAG	2 cut(s)	600, 654
Eco81I	CCTNAGG	2 cut(s)	194, 762
Eco88I	CYCGRG	2 cut(s)	72, 88
EcoICRI	GAGCTC	1 cut(s)	988
EcoNI	CCTNNNNNAGG	2 cut(s)	516, 938
EcoO109I	RGGNCCY	3 cut(s)	521, 842, 1027
EcoRI	GAATTC	1 cut(s)	66
EcoRII	CCWGG	2 cut(s)	478, 933
EcoRV	GATATC	1 cut(s)	769
EcoT14I	CCWWGG	1 cut(s)	306
EcoT22I	ATGCAT	1 cut(s)	1063
EcoT38I	GRGCYC	2 cut(s)	791, 990
EgeI	GGCGCC	1 cut(s)	900
EheI	GGCGCC	1 cut(s)	900
ErhI	CCWWGG	1 cut(s)	306
FaeI	CATG	5 cut(s)	148, 310, 467, 505, 732
FaqI	GGGAC	2 cut(s)	418, 546
FatI	CATG	5 cut(s)	144, 306, 463, 501, 728
FauI	CCCGC	1 cut(s)	554
FauNDI	CATATG	1 cut(s)	1057
FblI	GTMKAC	4 cut(s)	137, 535, 688, 702
Fnu4HI	GCNGC	2 cut(s)	372, 576
FokI	GGATG	3 cut(s)	145, 293, 1079
FriOI	GRGCYC	2 cut(s)	791, 990
Fsp4HI	GCNGC	2 cut(s)	372, 576
FspBI	CTAG	4 cut(s)	129, 375, 414, 420
GlaI	GCGC	2 cut(s)	210, 900
GluI	GCNGC	2 cut(s)	372, 576
HaeII	RGCGCY	1 cut(s)	902
HaeIII	GGCC	3 cut(s)	631, 789, 1028
HapII	CCGG	2 cut(s)	677, 785
HgaI	GACGC	2 cut(s)	420, 807
HhaI	GCGC	2 cut(s)	211, 901
Hin1I	GRCGYC	1 cut(s)	899
Hin1II	CATG	5 cut(s)	148, 310, 467, 505, 732
Hin6I	GCGC	2 cut(s)	209, 899
HinP1I	GCGC	2 cut(s)	209, 899
HincII	GTYRAC	1 cut(s)	689
HindII	GTYRAC	1 cut(s)	689
HinfI	GANTC	1 cut(s)	596
HpaII	CCGG	2 cut(s)	677, 785
HphI	GGTGA	4 cut(s)	155, 535, 949, 1082
Hpy166II	GTNNAC	5 cut(s)	138, 536, 621, 689, 703
Hpy188I	TCNGA	9 cut(s)	19, 280, 303, 319, 393, 530, 595, 1011, 1066
Hpy188III	TCNNGA	5 cut(s)	90, 435, 761, 795, 1019
Hpy8I	GTNNAC	5 cut(s)	138, 536, 621, 689, 703
Hpy99I	CGWCG	1 cut(s)	880
HpyAV	CCTTC	6 cut(s)	229, 233, 522, 603, 889, 934
HpyCH4III	ACNGT	2 cut(s)	185, 1103
HpyCH4IV	ACGT	1 cut(s)	590
HpyCH4V	TGCA	4 cut(s)	188, 965, 1061, 1099
HpyF10VI	GCNNNNNNNGC	4 cut(s)	185, 206, 469, 581
HpyF3I	CTNAG	7 cut(s)	194, 257, 269, 318, 390, 762, 807
HpySE526I	ACGT	1 cut(s)	590
Hsp92I	GRCGYC	1 cut(s)	899
Hsp92II	CATG	5 cut(s)	148, 310, 467, 505, 732
HspAI	GCGC	2 cut(s)	209, 899
KasI	GGCGCC	1 cut(s)	898
Kzo9I	GATC	8 cut(s)	40, 101, 742, 753, 781, 976, 1015, 1106
Lsp1109I	GCAGC	1 cut(s)	358
LweI	GCATC	2 cut(s)	1027, 1070
MaeI	CTAG	4 cut(s)	129, 375, 414, 420
MaeII	ACGT	1 cut(s)	590
MalI	GATC	8 cut(s)	42, 103, 744, 755, 783, 978, 1017, 1108
MboI	GATC	8 cut(s)	40, 101, 742, 753, 781, 976, 1015, 1106
MboII	GAAGA	4 cut(s)	35, 546, 647, 791
MflI	RGATCY	4 cut(s)	101, 753, 781, 976
MhlI	GDGCHC	3 cut(s)	791, 990, 1089
MluCI	AATT	6 cut(s)	66, 156, 313, 539, 914, 1005
Mly113I	GGCGCC	1 cut(s)	899
MmeI	TCCRAC	2 cut(s)	508, 586
Mph1103I	ATGCAT	1 cut(s)	1063
MseI	TTAA	4 cut(s)	204, 312, 747, 920
MslI	CAYNNNNRTG	1 cut(s)	733
MspI	CCGG	2 cut(s)	677, 785
MspR9I	CCNGG	4 cut(s)	480, 677, 786, 935
MvaI	CCWGG	2 cut(s)	480, 935
MwoI	GCNNNNNNNGC	4 cut(s)	185, 206, 469, 581
NarI	GGCGCC	1 cut(s)	899
NciI	CCSGG	2 cut(s)	677, 786
NcoI	CCATGG	1 cut(s)	306
NdeI	CATATG	1 cut(s)	1057
NdeII	GATC	8 cut(s)	40, 101, 742, 753, 781, 976, 1015, 1106
NlaIII	CATG	5 cut(s)	148, 310, 467, 505, 732
NlaIV	GGNNCC	4 cut(s)	562, 789, 844, 900
NsiI	ATGCAT	1 cut(s)	1063
PaeR7I	CTCGAG	1 cut(s)	72
PfeI	GAWTC	1 cut(s)	596
PflMI	CCANNNNNTGG	1 cut(s)	479
PkrI	GCNGC	2 cut(s)	373, 577
PluTI	GGCGCC	1 cut(s)	902
PpuMI	RGGWCCY	2 cut(s)	521, 842
Psp124BI	GAGCTC	1 cut(s)	990
Psp5II	RGGWCCY	2 cut(s)	521, 842
Psp6I	CCWGG	2 cut(s)	478, 933
PspGI	CCWGG	2 cut(s)	478, 933
PspN4I	GGNNCC	4 cut(s)	562, 789, 844, 900
PspOMI	GGGCCC	1 cut(s)	787
PspPI	GGNCC	6 cut(s)	521, 560, 787, 788, 842, 1027
PspPPI	RGGWCCY	2 cut(s)	521, 842
PspXI	VCTCGAGB	1 cut(s)	72
PstNI	CAGNNNCTG	1 cut(s)	524
PsuI	RGATCY	4 cut(s)	101, 753, 781, 976
RseI	CAYNNNNRTG	1 cut(s)	733
SacI	GAGCTC	1 cut(s)	990
SalI	GTCGAC	1 cut(s)	687
SaqAI	TTAA	4 cut(s)	204, 312, 747, 920
SatI	GCNGC	2 cut(s)	372, 576
Sau3AI	GATC	8 cut(s)	40, 101, 742, 753, 781, 976, 1015, 1106
Sau96I	GGNCC	6 cut(s)	521, 560, 787, 788, 842, 1027
ScrFI	CCNGG	4 cut(s)	480, 677, 786, 935
SduI	GDGCHC	3 cut(s)	791, 990, 1089
SfaNI	GCATC	2 cut(s)	1027, 1070
SfcI	CTRYAG	1 cut(s)	108
SfoI	GGCGCC	1 cut(s)	900
Sfr274I	CTCGAG	1 cut(s)	72
SinI	GGWCC	3 cut(s)	521, 560, 842
SlaI	CTCGAG	1 cut(s)	72
SmiMI	CAYNNNNRTG	1 cut(s)	733
SmlI	CTYRAG	2 cut(s)	72, 167
SmoI	CTYRAG	2 cut(s)	72, 167
Sse9I	AATT	6 cut(s)	66, 156, 313, 539, 914, 1005
SsiI	CCGC	2 cut(s)	547, 575
SspDI	GGCGCC	1 cut(s)	898
SspMI	CTAG	4 cut(s)	129, 375, 414, 420
SstI	GAGCTC	1 cut(s)	990
StyD4I	CCNGG	4 cut(s)	478, 675, 784, 933
StyI	CCWWGG	1 cut(s)	306
TaaI	ACNGT	2 cut(s)	185, 1103
TaiI	ACGT	1 cut(s)	593
TaqI	TCGA	5 cut(s)	73, 330, 688, 990, 1105
TasI	AATT	6 cut(s)	66, 156, 313, 539, 914, 1005
TauI	GCSGC	1 cut(s)	578
TfiI	GAWTC	1 cut(s)	596
Tru1I	TTAA	4 cut(s)	204, 312, 747, 920
Tru9I	TTAA	4 cut(s)	204, 312, 747, 920
TscAI	CASTG	2 cut(s)	31, 37
TseI	GCWGC	1 cut(s)	371
TspDTI	ATGAA	8 cut(s)	149, 300, 375, 417, 509, 518, 588, 666
TspGWI	ACGGA	1 cut(s)	864
TspRI	CASTG	2 cut(s)	31, 37
Van91I	CCANNNNNTGG	1 cut(s)	479
VpaK11BI	GGWCC	3 cut(s)	521, 560, 842
XagI	CCTNNNNNAGG	2 cut(s)	516, 938
XapI	RAATTY	3 cut(s)	66, 156, 1005
XcmI	CCANNNNNNNNNTGG	2 cut(s)	726, 1048
XhoI	CTCGAG	1 cut(s)	72
XmiI	GTMKAC	4 cut(s)	137, 535, 688, 702
XspI	CTAG	4 cut(s)	129, 375, 414, 420
Zsp2I	ATGCAT	1 cut(s)	1063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.