FvH4_6g26750

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012406
Physical Location & Seq
Reverse (-)
20441463 .. 20442825
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26750.t1

Sequence Viewer

Length: 675 bp
ATGAAGTTCACAGACTCGCCGGTGATAGAGCTCCCCGTCGGCGACGCCGTCCTCTCCTTCCAACAAGACAACGGCTCCCTCCACGTCGGCACCACCGTCTGGCCCTGCTCCTTAGTCCTCGTCAAGTTCGCCGAGCGCTGGGCCCCGCCCACCACCCACCCCCAAAACCCCTACTCCGACCTCCTTGATTTCCGCGCGAAACGCGCCGTCGAGCTCGGCGCCGGATGCGGAGTCGCCGCCATGGGGCTCTACCTCCTCGGCCTCACCGACGTCGTCATCACCGACATCCCCGCCGTGATGCCGGCGCTGAAGCGGAACCTCAAGCGCAACAAGCCCGTCCTCGGCAAGAACCTCAAGCACGCGATTCTCCACTGGAACAAGGCCGACCAGATCGCCGCCCTCAGCCCTCCGTACGACGTCGTTGTGGCCACCGACGTGGTTTACATCGAGGAGACGGTGGGCCCGCTGATCTCGACCATGGAGGCGATGGTGAAGGACGACGGCGTCGTTTTGCTCGGCTACCAATTGAGGTCGCCGGAGGCGCATAAGCTGTTCTGGGAAATGTGTGAAGGGGTTTTTTTTATCGAGAAAGTTCCGCACCAGGATTTGCACCCTGATTATGCGTATGAGGAGACCGACGTCTATATCTTGCGCAAGAAGAAACAGGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

225

Amino Acids

24.98

Weight (kDa)

5.63

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 16 - 188 4.7e-26 Lysine methyltransferase
Methyltransf_23 PF13489 63 - 185 3.5e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44170
fragaria_vesca FvH4_6g26750
malus_domestica MD03G1234000.v1.1 MD11G1254400.v1.1
prunus_persica Prupe.4G174700_v2.0.a1
pyrus_communis pycom03g18060
rosa_chinensis RchiOBHm_Chr0c19g0500101
rosa_laevigata RLG00000005980 RLG00000018989 RLG00000035646
rosa_multiflora Rmu_sc0014193.1_g000003
rosa_roxburghii Rroxscaffold_2G00115700 Rroxscaffold_2G00116140
rosa_rugosa Rorug02G0277800
rosa_samantha Rh2AG328900 Rh2BG338800 Rh2CG315800 Rh2DG355500
rosa_wichuraiana Rw2G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 503
AatII GACGTC 3 cut(s) 273, 420, 642
Acc16I TGCGCA 1 cut(s) 653
AccB1I GGYRCC 2 cut(s) 89, 218
AccB7I CCANNNNNTGG 1 cut(s) 99
AccII CGCG 4 cut(s) 195, 197, 204, 362
AciI CCGC 9 cut(s) 146, 193, 228, 237, 291, 313, 396, 464, 596
AcoI YGGCCR 1 cut(s) 426
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 6 cut(s) 45, 219, 270, 417, 504, 639
AfaI GTAC 1 cut(s) 413
AfeI AGCGCT 1 cut(s) 137
AfiI CCNNNNNNNGG 4 cut(s) 99, 138, 243, 341
AjiI CACGTC 2 cut(s) 85, 436
AjnI CCWGG 1 cut(s) 600
AleI CACNNNNGTG 1 cut(s) 434
AluBI AGCT 3 cut(s) 31, 214, 550
AluI AGCT 3 cut(s) 31, 214, 550
Alw21I GWGCWC 2 cut(s) 33, 216
Alw26I GTCTC 2 cut(s) 446, 626
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 6 cut(s) 101, 141, 259, 381, 426, 460
ApaI GGGCCC 2 cut(s) 145, 464
AspLEI GCGC 8 cut(s) 138, 197, 206, 221, 307, 327, 544, 654
AspS9I GGNCC 5 cut(s) 102, 141, 142, 460, 461
AsuHPI GGTGA 4 cut(s) 34, 256, 271, 502
BaeGI GKGCMC 2 cut(s) 145, 464
BalI TGGCCA 1 cut(s) 428
BanI GGYRCC 2 cut(s) 89, 218
BanII GRGCYC 5 cut(s) 33, 145, 216, 249, 464
Bbv12I GWGCWC 2 cut(s) 33, 216
BbvCI CCTCAGC 1 cut(s) 401
BccI CCATC 1 cut(s) 481
BceAI ACGGC 5 cut(s) 32, 88, 191, 278, 517
BciT130I CCWGG 1 cut(s) 602
BcoDI GTCTC 2 cut(s) 446, 626
BfoI RGCGCY 3 cut(s) 139, 222, 308
BisI GCNGC 2 cut(s) 237, 396
BlsI GCNGC 2 cut(s) 238, 397
Bme1390I CCNGG 1 cut(s) 602
BmgBI CACGTC 2 cut(s) 85, 436
BmgT120I GGNCC 5 cut(s) 102, 141, 142, 460, 461
BmiI GGNNCC 7 cut(s) 76, 91, 143, 144, 220, 317, 462
BmrFI CCNGG 1 cut(s) 602
BmsI GCATC 2 cut(s) 215, 288
BoxI GACNNNNGTC 1 cut(s) 638
Bpu10I CCTNAGC 1 cut(s) 401
BpuEI CTTGAG 2 cut(s) 305, 338
BsaHI GRCGYC 6 cut(s) 45, 219, 270, 417, 504, 639
BsaI GGTCTC 1 cut(s) 626
BsaJI CCNNGG 4 cut(s) 240, 256, 340, 477
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 158, 188
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 138, 243, 341
Bse118I RCCGGY 2 cut(s) 19, 301
Bse1I ACTGG 1 cut(s) 377
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 4 cut(s) 240, 256, 340, 477
BseGI GGATG 2 cut(s) 230, 285
BseLI CCNNNNNNNGG 4 cut(s) 99, 138, 243, 341
BseMII CTCAG 1 cut(s) 415
BseNI ACTGG 1 cut(s) 377
BseRI GAGGAG 3 cut(s) 245, 464, 644
BseSI GKGCMC 2 cut(s) 145, 464
BseYI CCCAGC 1 cut(s) 138
Bsh1236I CGCG 4 cut(s) 195, 197, 204, 362
BshFI GGCC 6 cut(s) 103, 143, 261, 383, 428, 462
BshNI GGYRCC 2 cut(s) 89, 218
BsiHKAI GWGCWC 2 cut(s) 33, 216
BsiSI CCGG 4 cut(s) 20, 222, 302, 536
BsiWI CGTACG 1 cut(s) 411
BslI CCNNNNNNNGG 4 cut(s) 99, 138, 243, 341
BsmAI GTCTC 2 cut(s) 446, 626
BsmBI CGTCTC 1 cut(s) 446
BsnI GGCC 6 cut(s) 103, 143, 261, 383, 428, 462
Bso31I GGTCTC 1 cut(s) 626
Bsp120I GGGCCC 2 cut(s) 141, 460
Bsp1286I GDGCHC 5 cut(s) 33, 145, 216, 249, 464
Bsp143I GATC 2 cut(s) 390, 468
Bsp19I CCATGG 2 cut(s) 240, 477
BspACI CCGC 9 cut(s) 146, 193, 228, 237, 291, 313, 396, 464, 596
BspANI GGCC 6 cut(s) 103, 143, 261, 383, 428, 462
BspCNI CTCAG 1 cut(s) 414
BspFNI CGCG 4 cut(s) 195, 197, 204, 362
BspLI GGNNCC 7 cut(s) 76, 91, 143, 144, 220, 317, 462
BspT107I GGYRCC 2 cut(s) 89, 218
BspTNI GGTCTC 1 cut(s) 626
BsrFI RCCGGY 2 cut(s) 19, 301
BsrI ACTGG 1 cut(s) 377
BssAI RCCGGY 2 cut(s) 19, 301
BssECI CCNNGG 4 cut(s) 240, 256, 340, 477
BssMI GATC 2 cut(s) 390, 468
BssNI GRCGYC 6 cut(s) 45, 219, 270, 417, 504, 639
BssT1I CCWWGG 2 cut(s) 240, 477
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 2 cut(s) 97, 457
BstACI GRCGYC 6 cut(s) 45, 219, 270, 417, 504, 639
BstC8I GCNNGC 3 cut(s) 303, 360, 464
BstDEI CTNAG 2 cut(s) 112, 401
BstDSI CCRYGG 2 cut(s) 240, 477
BstF5I GGATG 2 cut(s) 230, 285
BstFNI CGCG 4 cut(s) 195, 197, 204, 362
BstH2I RGCGCY 3 cut(s) 139, 222, 308
BstHHI GCGC 8 cut(s) 138, 197, 206, 221, 307, 327, 544, 654
BstKTI GATC 2 cut(s) 393, 471
BstMAI GTCTC 2 cut(s) 446, 626
BstMBI GATC 2 cut(s) 390, 468
BstMWI GCNNNNNNNGC 5 cut(s) 201, 203, 225, 331, 541
BstNI CCWGG 1 cut(s) 602
BstPAI GACNNNNGTC 1 cut(s) 638
BstSCI CCNGG 1 cut(s) 600
BstSLI GKGCMC 2 cut(s) 145, 464
BstUI CGCG 4 cut(s) 195, 197, 204, 362
BstXI CCANNNNNNTGG 1 cut(s) 436
BsuRI GGCC 6 cut(s) 103, 143, 261, 383, 428, 462
BtgI CCRYGG 2 cut(s) 240, 477
BtgZI GCGATG 1 cut(s) 500
BtrI CACGTC 2 cut(s) 85, 436
BtsCI GGATG 2 cut(s) 230, 285
BtsIMutI CAGTG 1 cut(s) 370
Cac8I GCNNGC 3 cut(s) 303, 360, 464
CfoI GCGC 8 cut(s) 138, 197, 206, 221, 307, 327, 544, 654
Cfr10I RCCGGY 2 cut(s) 19, 301
Cfr13I GGNCC 5 cut(s) 102, 141, 142, 460, 461
CseI GACGC 2 cut(s) 53, 493
Csp6I GTAC 1 cut(s) 412
CviAII CATG 2 cut(s) 241, 478
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 2 cut(s) 112, 401
DinI GGCGCC 1 cut(s) 220
DpnI GATC 2 cut(s) 392, 470
DpnII GATC 2 cut(s) 390, 468
DrdI GACNNNNNNGTC 1 cut(s) 503
DseDI GACNNNNNNGTC 1 cut(s) 503
EaeI YGGCCR 1 cut(s) 426
Ecl136II GAGCTC 2 cut(s) 31, 214
Eco130I CCWWGG 2 cut(s) 240, 477
Eco24I GRGCYC 5 cut(s) 33, 145, 216, 249, 464
Eco31I GGTCTC 1 cut(s) 626
Eco47III AGCGCT 1 cut(s) 137
Eco53kI GAGCTC 2 cut(s) 31, 214
Eco57I CTGAAG 1 cut(s) 329
EcoICRI GAGCTC 2 cut(s) 31, 214
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 600
EcoT14I CCWWGG 2 cut(s) 240, 477
EcoT38I GRGCYC 5 cut(s) 33, 145, 216, 249, 464
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 2 cut(s) 240, 477
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 2 cut(s) 244, 481
FaiI YATR 6 cut(s) 242, 479, 546, 621, 627, 645
FatI CATG 2 cut(s) 240, 477
FauI CCCGC 3 cut(s) 153, 298, 471
Fnu4HI GCNGC 2 cut(s) 237, 396
FokI GGATG 2 cut(s) 237, 272
FriOI GRGCYC 5 cut(s) 33, 145, 216, 249, 464
Fsp4HI GCNGC 2 cut(s) 237, 396
FspI TGCGCA 1 cut(s) 653
GlaI GCGC 8 cut(s) 137, 196, 205, 220, 306, 326, 543, 653
GluI GCNGC 2 cut(s) 237, 396
GsaI CCCAGC 1 cut(s) 142
HaeII RGCGCY 3 cut(s) 139, 222, 308
HaeIII GGCC 6 cut(s) 103, 143, 261, 383, 428, 462
HapII CCGG 4 cut(s) 20, 222, 302, 536
HgaI GACGC 2 cut(s) 53, 493
HhaI GCGC 8 cut(s) 138, 197, 206, 221, 307, 327, 544, 654
Hin1I GRCGYC 6 cut(s) 45, 219, 270, 417, 504, 639
Hin1II CATG 2 cut(s) 244, 481
Hin6I GCGC 8 cut(s) 136, 195, 204, 219, 305, 325, 542, 652
HinP1I GCGC 8 cut(s) 136, 195, 204, 219, 305, 325, 542, 652
HinfI GANTC 3 cut(s) 14, 231, 364
HpaII CCGG 4 cut(s) 20, 222, 302, 536
HphI GGTGA 4 cut(s) 34, 256, 271, 502
Hpy166II GTNNAC 2 cut(s) 9, 442
Hpy188I TCNGA 2 cut(s) 178, 674
Hpy188III TCNNGA 2 cut(s) 472, 586
Hpy8I GTNNAC 2 cut(s) 9, 442
HpyAV CCTTC 3 cut(s) 67, 487, 563
HpyCH4III ACNGT 2 cut(s) 97, 457
HpyCH4IV ACGT 5 cut(s) 84, 270, 417, 435, 639
HpyCH4V TGCA 1 cut(s) 610
HpyF10VI GCNNNNNNNGC 5 cut(s) 201, 203, 225, 331, 541
HpyF3I CTNAG 2 cut(s) 112, 401
HpySE526I ACGT 5 cut(s) 84, 270, 417, 435, 639
Hsp92I GRCGYC 6 cut(s) 45, 219, 270, 417, 504, 639
Hsp92II CATG 2 cut(s) 244, 481
HspAI GCGC 8 cut(s) 136, 195, 204, 219, 305, 325, 542, 652
KasI GGCGCC 1 cut(s) 218
KroI GCCGGC 1 cut(s) 301
KroNI GCCGGC 1 cut(s) 303
Kzo9I GATC 2 cut(s) 390, 468
LmnI GCTCC 3 cut(s) 36, 80, 113
LweI GCATC 2 cut(s) 215, 288
MaeII ACGT 5 cut(s) 84, 270, 417, 435, 639
MalI GATC 2 cut(s) 392, 470
MboI GATC 2 cut(s) 390, 468
MboII GAAGA 1 cut(s) 670
MfeI CAATTG 1 cut(s) 524
MhlI GDGCHC 5 cut(s) 33, 145, 216, 249, 464
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 1 cut(s) 524
MluNI TGGCCA 1 cut(s) 428
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 2 cut(s) 8, 240
MmeI TCCRAC 2 cut(s) 85, 201
Mox20I TGGCCA 1 cut(s) 428
MroNI GCCGGC 1 cut(s) 301
MscI TGGCCA 1 cut(s) 428
MslI CAYNNNNRTG 1 cut(s) 434
Msp20I TGGCCA 1 cut(s) 428
MspA1I CMGCKG 1 cut(s) 466
MspI CCGG 4 cut(s) 20, 222, 302, 536
MspR9I CCNGG 1 cut(s) 602
MunI CAATTG 1 cut(s) 524
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 4 cut(s) 195, 197, 204, 362
MwoI GCNNNNNNNGC 5 cut(s) 201, 203, 225, 331, 541
NaeI GCCGGC 1 cut(s) 303
NarI GGCGCC 1 cut(s) 219
NcoI CCATGG 2 cut(s) 240, 477
NdeII GATC 2 cut(s) 390, 468
NgoMIV GCCGGC 1 cut(s) 301
NlaIII CATG 2 cut(s) 244, 481
NlaIV GGNNCC 7 cut(s) 76, 91, 143, 144, 220, 317, 462
NmeAIII GCCGAG 5 cut(s) 157, 195, 237, 321, 495
NsbI TGCGCA 1 cut(s) 653
OliI CACNNNNGTG 1 cut(s) 434
PcsI WCGNNNNNNNCGW 5 cut(s) 45, 93, 264, 279, 504
PdiI GCCGGC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 364
Pfl23II CGTACG 1 cut(s) 411
PflFI GACNNNGTC 3 cut(s) 47, 272, 503
PflMI CCANNNNNTGG 1 cut(s) 99
PkrI GCNGC 2 cut(s) 238, 397
PleI GAGTC 2 cut(s) 8, 239
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 2 cut(s) 8, 239
PshAI GACNNNNGTC 1 cut(s) 638
Psp124BI GAGCTC 2 cut(s) 33, 216
Psp6I CCWGG 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 138
PspGI CCWGG 1 cut(s) 600
PspLI CGTACG 1 cut(s) 411
PspN4I GGNNCC 7 cut(s) 76, 91, 143, 144, 220, 317, 462
PspOMI GGGCCC 2 cut(s) 141, 460
PspPI GGNCC 5 cut(s) 102, 141, 142, 460, 461
PsyI GACNNNGTC 3 cut(s) 47, 272, 503
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 1 cut(s) 434
SacI GAGCTC 2 cut(s) 33, 216
SatI GCNGC 2 cut(s) 237, 396
Sau3AI GATC 2 cut(s) 390, 468
Sau96I GGNCC 5 cut(s) 102, 141, 142, 460, 461
SchI GAGTC 2 cut(s) 8, 240
ScrFI CCNGG 1 cut(s) 602
SduI GDGCHC 5 cut(s) 33, 145, 216, 249, 464
SfaNI GCATC 2 cut(s) 215, 288
SfoI GGCGCC 1 cut(s) 220
SgrAI CRCCGGYG 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 2 cut(s) 320, 353
SmoI CTYRAG 2 cut(s) 320, 353
Sse9I AATT 1 cut(s) 524
SsiI CCGC 9 cut(s) 146, 193, 228, 237, 291, 313, 396, 464, 596
SspDI GGCGCC 1 cut(s) 218
SstI GAGCTC 2 cut(s) 33, 216
StyD4I CCNGG 1 cut(s) 600
StyI CCWWGG 2 cut(s) 240, 477
TaaI ACNGT 2 cut(s) 97, 457
TaiI ACGT 5 cut(s) 87, 273, 420, 438, 642
TaqI TCGA 4 cut(s) 210, 447, 473, 585
TaqII GACCGA 1 cut(s) 650
TasI AATT 1 cut(s) 524
TauI GCSGC 2 cut(s) 239, 398
TfiI GAWTC 1 cut(s) 364
TscAI CASTG 1 cut(s) 377
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 1 cut(s) 377
Tth111I GACNNNGTC 3 cut(s) 47, 272, 503
Van91I CCANNNNNTGG 1 cut(s) 99
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
ZraI GACGTC 3 cut(s) 271, 418, 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.