Rw2G026660

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012406
Physical Location & Seq
Reverse (-)
41374861 .. 41376401
1541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G026660.1

Sequence Viewer

Length: 807 bp
ATGAAAAACCTTATTGGCTTTTATCAAACTGGGCCTCTATTTCGGTTAAAGTCCGAAAATACAAATGTTGACGCTTTGCCTCATTTTTTTGGGCAAAACCCAGAATCTAAACAAATCGGCGGCAAAGGTATGAAGTTCACAGACTCGCCGGTGATAGAGCTCCCCGTCCGCGACGCCGTTCTCTCCTTCCAACAAGACAACGGCTCCCTCCATGTCGGCACCACCGTCTGGCCCTGCTCCTTAGTCCTCGTCAAGTTCGCCGAGCGCTGGGCCCCACCAAGCAACCACCAACCCCAAAACCCCTATTCCGACCTCCTCGACTTCCGCGCCAAGCGCGCCGTCGAGCTCGGCGCCGGATGCGGAGTCGCCGCCATGGGGCTTTACCTCCTCGGCCTGACCGACGTCGTCATCACCGACATCCCCGCCGTGATGCCGGCGCTGAAGCGAAACCTCAAGCGCAACAAACCGGTCCTCGGCAAGAACCTCAAGCACGCGATTCTCCACTGGAACAAGGCGGACCAGATCGCCGCGCTCAGCCCTCCGTACGACGTCGTTGTGGCGACCGATGTGGTTTATATTGAGGAGACCGTAGGACCCTTGATCTCCACCATGGAGGCGATGGTGAAGGACGACGGCGTCGTTTTGCTTGGGTACCAATTGAGGTCGCCGGAGGCGCATAAGCTCTTCTGGGAAATGTGTGAAGGGGTTTTTGTGATCGAGAAAGTTCCGCACCAGGATTTGCACCCGGATTATGCGTATGAGGAGACCGATGTCTATATCTTGCGCAAGAAGAAAAAGGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

268

Amino Acids

29.92

Weight (kDa)

6.45

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 59 - 232 9.8e-26 Lysine methyltransferase
Methyltransf_23 PF13489 107 - 229 4.9e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44170
fragaria_vesca FvH4_6g26750
malus_domestica MD03G1234000.v1.1 MD11G1254400.v1.1
prunus_persica Prupe.4G174700_v2.0.a1
pyrus_communis pycom03g18060
rosa_chinensis RchiOBHm_Chr0c19g0500101
rosa_laevigata RLG00000005980 RLG00000018989 RLG00000035646
rosa_multiflora Rmu_sc0014193.1_g000003
rosa_roxburghii Rroxscaffold_2G00115700 Rroxscaffold_2G00116140
rosa_rugosa Rorug02G0277800
rosa_samantha Rh2AG328900 Rh2BG338800 Rh2CG315800 Rh2DG355500
rosa_wichuraiana Rw2G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 635
AatII GACGTC 2 cut(s) 405, 552
Acc16I TGCGCA 1 cut(s) 785
Acc65I GGTACC 1 cut(s) 651
AccB1I GGYRCC 3 cut(s) 218, 350, 651
AccB7I CCANNNNNTGG 1 cut(s) 228
AccII CGCG 5 cut(s) 171, 327, 336, 494, 530
AciI CCGC 9 cut(s) 120, 169, 325, 360, 369, 423, 515, 528, 728
AcuI CTGAAG 1 cut(s) 461
AcyI GRCGYC 5 cut(s) 174, 351, 402, 549, 636
AfaI GTAC 2 cut(s) 545, 653
AfeI AGCGCT 1 cut(s) 266
AfiI CCNNNNNNNGG 4 cut(s) 228, 267, 375, 473
AgeI ACCGGT 1 cut(s) 466
AjnI CCWGG 1 cut(s) 732
AluBI AGCT 3 cut(s) 160, 346, 682
AluI AGCT 3 cut(s) 160, 346, 682
Alw21I GWGCWC 2 cut(s) 162, 348
Alw26I GTCTC 2 cut(s) 578, 758
Aor51HI AGCGCT 1 cut(s) 266
AoxI GGCC 4 cut(s) 32, 230, 270, 391
ApaI GGGCCC 1 cut(s) 274
AsiGI ACCGGT 1 cut(s) 466
Asp718I GGTACC 1 cut(s) 651
AspS9I GGNCC 7 cut(s) 32, 231, 270, 271, 469, 517, 593
AsuC2I CCSGG 1 cut(s) 746
AsuHPI GGTGA 3 cut(s) 163, 403, 634
AvaII GGWCC 3 cut(s) 469, 517, 593
BaeGI GKGCMC 1 cut(s) 274
BanI GGYRCC 3 cut(s) 218, 350, 651
BanII GRGCYC 3 cut(s) 162, 274, 348
Bbv12I GWGCWC 2 cut(s) 162, 348
BccI CCATC 1 cut(s) 613
BceAI ACGGC 5 cut(s) 161, 217, 323, 410, 649
BciT130I CCWGG 1 cut(s) 734
BcnI CCSGG 1 cut(s) 746
BcoDI GTCTC 2 cut(s) 578, 758
BfoI RGCGCY 3 cut(s) 268, 354, 440
BisI GCNGC 3 cut(s) 121, 369, 528
BlpI GCTNAGC 1 cut(s) 533
BlsI GCNGC 3 cut(s) 122, 370, 529
Bme1390I CCNGG 2 cut(s) 734, 746
Bme18I GGWCC 3 cut(s) 469, 517, 593
BmgT120I GGNCC 7 cut(s) 32, 231, 270, 271, 469, 517, 593
BmiI GGNNCC 7 cut(s) 205, 220, 272, 273, 352, 595, 653
BmrFI CCNGG 2 cut(s) 734, 746
BmrI ACTGGG 1 cut(s) 39
BmsI GCATC 2 cut(s) 347, 420
BmuI ACTGGG 1 cut(s) 39
BoxI GACNNNNGTC 2 cut(s) 401, 770
Bpu1102I GCTNAGC 1 cut(s) 533
BpuEI CTTGAG 2 cut(s) 437, 470
BpuMI CCSGG 1 cut(s) 746
BsaHI GRCGYC 5 cut(s) 174, 351, 402, 549, 636
BsaI GGTCTC 2 cut(s) 578, 758
BsaJI CCNNGG 4 cut(s) 372, 388, 472, 609
BsaWI WCCGGW 1 cut(s) 466
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 267, 375, 473
Bse118I RCCGGY 3 cut(s) 148, 433, 466
Bse1I ACTGG 2 cut(s) 34, 509
BseBI CCWGG 1 cut(s) 734
BseDI CCNNGG 4 cut(s) 372, 388, 472, 609
BseGI GGATG 2 cut(s) 362, 417
BseLI CCNNNNNNNGG 4 cut(s) 228, 267, 375, 473
BseMII CTCAG 1 cut(s) 547
BseNI ACTGG 2 cut(s) 34, 509
BsePI GCGCGC 1 cut(s) 334
BseRI GAGGAG 4 cut(s) 305, 377, 596, 776
BseSI GKGCMC 1 cut(s) 274
BseYI CCCAGC 1 cut(s) 267
Bsh1236I CGCG 5 cut(s) 171, 327, 336, 494, 530
Bsh1285I CGRYCG 1 cut(s) 564
BshFI GGCC 4 cut(s) 34, 232, 272, 393
BshNI GGYRCC 3 cut(s) 218, 350, 651
BshTI ACCGGT 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 564
BsiHKAI GWGCWC 2 cut(s) 162, 348
BsiSI CCGG 6 cut(s) 149, 354, 434, 467, 668, 746
BsiWI CGTACG 1 cut(s) 543
BslI CCNNNNNNNGG 4 cut(s) 228, 267, 375, 473
BsmAI GTCTC 2 cut(s) 578, 758
BsnI GGCC 4 cut(s) 34, 232, 272, 393
Bso31I GGTCTC 2 cut(s) 578, 758
Bsp120I GGGCCC 1 cut(s) 270
Bsp1286I GDGCHC 3 cut(s) 162, 274, 348
Bsp143I GATC 3 cut(s) 522, 600, 714
Bsp1720I GCTNAGC 1 cut(s) 533
Bsp19I CCATGG 2 cut(s) 372, 609
BspACI CCGC 9 cut(s) 120, 169, 325, 360, 369, 423, 515, 528, 728
BspANI GGCC 4 cut(s) 34, 232, 272, 393
BspCNI CTCAG 1 cut(s) 546
BspFNI CGCG 5 cut(s) 171, 327, 336, 494, 530
BspLI GGNNCC 7 cut(s) 205, 220, 272, 273, 352, 595, 653
BspQI GCTCTTC 1 cut(s) 689
BspT107I GGYRCC 3 cut(s) 218, 350, 651
BspTNI GGTCTC 2 cut(s) 578, 758
BsrFI RCCGGY 3 cut(s) 148, 433, 466
BsrI ACTGG 2 cut(s) 34, 509
BssAI RCCGGY 3 cut(s) 148, 433, 466
BssECI CCNNGG 4 cut(s) 372, 388, 472, 609
BssHII GCGCGC 1 cut(s) 334
BssMI GATC 3 cut(s) 522, 600, 714
BssNI GRCGYC 5 cut(s) 174, 351, 402, 549, 636
BssT1I CCWWGG 2 cut(s) 372, 609
Bst2UI CCWGG 1 cut(s) 734
Bst4CI ACNGT 2 cut(s) 226, 589
Bst6I CTCTTC 1 cut(s) 689
BstACI GRCGYC 5 cut(s) 174, 351, 402, 549, 636
BstC8I GCNNGC 3 cut(s) 336, 435, 492
BstDEI CTNAG 2 cut(s) 241, 533
BstDSI CCRYGG 2 cut(s) 372, 609
BstF5I GGATG 2 cut(s) 362, 417
BstFNI CGCG 5 cut(s) 171, 327, 336, 494, 530
BstH2I RGCGCY 3 cut(s) 268, 354, 440
BstKTI GATC 3 cut(s) 525, 603, 717
BstMAI GTCTC 2 cut(s) 578, 758
BstMBI GATC 3 cut(s) 522, 600, 714
BstMCI CGRYCG 1 cut(s) 564
BstMWI GCNNNNNNNGC 4 cut(s) 333, 335, 357, 673
BstNI CCWGG 1 cut(s) 734
BstPAI GACNNNNGTC 2 cut(s) 401, 770
BstSCI CCNGG 2 cut(s) 732, 744
BstSLI GKGCMC 1 cut(s) 274
BstUI CGCG 5 cut(s) 171, 327, 336, 494, 530
BsuRI GGCC 4 cut(s) 34, 232, 272, 393
BtgI CCRYGG 2 cut(s) 372, 609
BtgZI GCGATG 1 cut(s) 632
BtsCI GGATG 2 cut(s) 362, 417
BtsIMutI CAGTG 1 cut(s) 502
Cac8I GCNNGC 3 cut(s) 336, 435, 492
Cfr10I RCCGGY 3 cut(s) 148, 433, 466
Cfr13I GGNCC 7 cut(s) 32, 231, 270, 271, 469, 517, 593
CseI GACGC 3 cut(s) 80, 182, 625
Csp6I GTAC 2 cut(s) 544, 652
CspAI ACCGGT 1 cut(s) 466
CviAII CATG 3 cut(s) 212, 373, 610
CviQI GTAC 2 cut(s) 544, 652
DdeI CTNAG 2 cut(s) 241, 533
DinI GGCGCC 1 cut(s) 352
DpnI GATC 3 cut(s) 524, 602, 716
DpnII GATC 3 cut(s) 522, 600, 714
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
EciI GGCGGA 1 cut(s) 530
Ecl136II GAGCTC 2 cut(s) 160, 346
Eco130I CCWWGG 2 cut(s) 372, 609
Eco24I GRGCYC 3 cut(s) 162, 274, 348
Eco31I GGTCTC 2 cut(s) 578, 758
Eco47I GGWCC 3 cut(s) 469, 517, 593
Eco47III AGCGCT 1 cut(s) 266
Eco53kI GAGCTC 2 cut(s) 160, 346
Eco57I CTGAAG 1 cut(s) 461
EcoICRI GAGCTC 2 cut(s) 160, 346
EcoO109I RGGNCCY 2 cut(s) 271, 593
EcoRII CCWGG 1 cut(s) 732
EcoT14I CCWWGG 2 cut(s) 372, 609
EcoT38I GRGCYC 3 cut(s) 162, 274, 348
EgeI GGCGCC 1 cut(s) 352
EheI GGCGCC 1 cut(s) 352
ErhI CCWWGG 2 cut(s) 372, 609
FaeI CATG 3 cut(s) 215, 376, 613
FaiI YATR 9 cut(s) 131, 213, 374, 576, 611, 678, 753, 759, 777
FatI CATG 3 cut(s) 211, 372, 609
FauI CCCGC 1 cut(s) 430
Fnu4HI GCNGC 3 cut(s) 121, 369, 528
FokI GGATG 2 cut(s) 369, 404
FriOI GRGCYC 3 cut(s) 162, 274, 348
Fsp4HI GCNGC 3 cut(s) 121, 369, 528
FspI TGCGCA 1 cut(s) 785
GluI GCNGC 3 cut(s) 121, 369, 528
GsaI CCCAGC 1 cut(s) 271
HaeII RGCGCY 3 cut(s) 268, 354, 440
HaeIII GGCC 4 cut(s) 34, 232, 272, 393
HapII CCGG 6 cut(s) 149, 354, 434, 467, 668, 746
HgaI GACGC 3 cut(s) 80, 182, 625
Hin1I GRCGYC 5 cut(s) 174, 351, 402, 549, 636
Hin1II CATG 3 cut(s) 215, 376, 613
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 4 cut(s) 104, 143, 363, 496
HpaII CCGG 6 cut(s) 149, 354, 434, 467, 668, 746
HphI GGTGA 3 cut(s) 163, 403, 634
Hpy166II GTNNAC 2 cut(s) 70, 138
Hpy188I TCNGA 2 cut(s) 55, 310
Hpy188III TCNNGA 1 cut(s) 718
Hpy8I GTNNAC 2 cut(s) 70, 138
Hpy99I CGWCG 8 cut(s) 176, 344, 404, 407, 551, 554, 635, 641
HpyAV CCTTC 3 cut(s) 196, 619, 695
HpyCH4III ACNGT 2 cut(s) 226, 589
HpyCH4IV ACGT 2 cut(s) 402, 549
HpyCH4V TGCA 1 cut(s) 742
HpyF10VI GCNNNNNNNGC 4 cut(s) 333, 335, 357, 673
HpyF3I CTNAG 2 cut(s) 241, 533
HpySE526I ACGT 2 cut(s) 402, 549
Hsp92I GRCGYC 5 cut(s) 174, 351, 402, 549, 636
Hsp92II CATG 3 cut(s) 215, 376, 613
KasI GGCGCC 1 cut(s) 350
KpnI GGTACC 1 cut(s) 655
KroI GCCGGC 1 cut(s) 433
KroNI GCCGGC 1 cut(s) 435
Kzo9I GATC 3 cut(s) 522, 600, 714
LguI GCTCTTC 1 cut(s) 689
LmnI GCTCC 3 cut(s) 165, 209, 242
LweI GCATC 2 cut(s) 347, 420
MaeII ACGT 2 cut(s) 402, 549
MalI GATC 3 cut(s) 524, 602, 716
MboI GATC 3 cut(s) 522, 600, 714
MboII GAAGA 2 cut(s) 676, 802
MfeI CAATTG 1 cut(s) 656
MhlI GDGCHC 3 cut(s) 162, 274, 348
MluCI AATT 1 cut(s) 656
Mly113I GGCGCC 1 cut(s) 351
MlyI GAGTC 2 cut(s) 137, 372
MmeI TCCRAC 2 cut(s) 214, 333
MroNI GCCGGC 1 cut(s) 433
MseI TTAA 1 cut(s) 47
MspI CCGG 6 cut(s) 149, 354, 434, 467, 668, 746
MspR9I CCNGG 2 cut(s) 734, 746
MunI CAATTG 1 cut(s) 656
MvaI CCWGG 1 cut(s) 734
MvnI CGCG 5 cut(s) 171, 327, 336, 494, 530
MwoI GCNNNNNNNGC 4 cut(s) 333, 335, 357, 673
NaeI GCCGGC 1 cut(s) 435
NarI GGCGCC 1 cut(s) 351
NciI CCSGG 1 cut(s) 746
NcoI CCATGG 2 cut(s) 372, 609
NdeII GATC 3 cut(s) 522, 600, 714
NgoMIV GCCGGC 1 cut(s) 433
NlaIII CATG 3 cut(s) 215, 376, 613
NlaIV GGNNCC 7 cut(s) 205, 220, 272, 273, 352, 595, 653
NmeAIII GCCGAG 4 cut(s) 286, 327, 369, 453
NsbI TGCGCA 1 cut(s) 785
PauI GCGCGC 1 cut(s) 334
PciSI GCTCTTC 1 cut(s) 689
PcsI WCGNNNNNNNCGW 4 cut(s) 222, 396, 411, 636
PdiI GCCGGC 1 cut(s) 435
PfeI GAWTC 2 cut(s) 104, 496
Pfl23II CGTACG 1 cut(s) 543
PflFI GACNNNGTC 2 cut(s) 404, 635
PflMI CCANNNNNTGG 1 cut(s) 228
PinAI ACCGGT 1 cut(s) 466
PkrI GCNGC 3 cut(s) 122, 370, 529
PleI GAGTC 2 cut(s) 137, 371
PluTI GGCGCC 1 cut(s) 354
PpsI GAGTC 2 cut(s) 137, 371
PpuMI RGGWCCY 1 cut(s) 593
PshAI GACNNNNGTC 2 cut(s) 401, 770
Psp124BI GAGCTC 2 cut(s) 162, 348
Psp5II RGGWCCY 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 732
PspFI CCCAGC 1 cut(s) 267
PspGI CCWGG 1 cut(s) 732
PspLI CGTACG 1 cut(s) 543
PspN4I GGNNCC 7 cut(s) 205, 220, 272, 273, 352, 595, 653
PspOMI GGGCCC 1 cut(s) 270
PspPI GGNCC 7 cut(s) 32, 231, 270, 271, 469, 517, 593
PspPPI RGGWCCY 1 cut(s) 593
PsyI GACNNNGTC 2 cut(s) 404, 635
PteI GCGCGC 1 cut(s) 334
RsaI GTAC 2 cut(s) 545, 653
RsaNI GTAC 2 cut(s) 544, 652
SacI GAGCTC 2 cut(s) 162, 348
SapI GCTCTTC 1 cut(s) 689
SaqAI TTAA 1 cut(s) 47
SatI GCNGC 3 cut(s) 121, 369, 528
Sau3AI GATC 3 cut(s) 522, 600, 714
Sau96I GGNCC 7 cut(s) 32, 231, 270, 271, 469, 517, 593
SchI GAGTC 2 cut(s) 137, 372
ScrFI CCNGG 2 cut(s) 734, 746
SduI GDGCHC 3 cut(s) 162, 274, 348
SfaNI GCATC 2 cut(s) 347, 420
SfoI GGCGCC 1 cut(s) 352
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 3 cut(s) 469, 517, 593
SmlI CTYRAG 2 cut(s) 452, 485
SmoI CTYRAG 2 cut(s) 452, 485
Sse9I AATT 1 cut(s) 656
SsiI CCGC 9 cut(s) 120, 169, 325, 360, 369, 423, 515, 528, 728
SspDI GGCGCC 1 cut(s) 350
SstI GAGCTC 2 cut(s) 162, 348
StyD4I CCNGG 2 cut(s) 732, 744
StyI CCWWGG 2 cut(s) 372, 609
TaaI ACNGT 2 cut(s) 226, 589
TaiI ACGT 2 cut(s) 405, 552
TaqI TCGA 3 cut(s) 318, 342, 717
TaqII GACCGA 3 cut(s) 413, 578, 782
TasI AATT 1 cut(s) 656
TauI GCSGC 3 cut(s) 123, 371, 530
TfiI GAWTC 2 cut(s) 104, 496
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TscAI CASTG 1 cut(s) 509
TspDTI ATGAA 2 cut(s) 17, 146
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 1 cut(s) 509
Tth111I GACNNNGTC 2 cut(s) 404, 635
Van91I CCANNNNNTGG 1 cut(s) 228
VpaK11BI GGWCC 3 cut(s) 469, 517, 593
XcmI CCANNNNNNNNNTGG 1 cut(s) 616
ZraI GACGTC 2 cut(s) 403, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.