Rh2BG338800

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012406
Physical Location & Seq
Reverse (-)
44907268 .. 44910628
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG338800.1

Sequence Viewer

Length: 678 bp
ATGAAGTTCACAGACTCGCCGGTGATAGAGCTCCCCGTCCGCGACGCCGTCCTCTCCTTCCAACAAGACAACGGCTCCCTCCATGTCGGCACCACCGTCTGGCCCTGCTCCTTAGTCCTCGTTAAGTTCGCCGAGCGCTGGGCCCCACCAAGCAACCACCAACCCCAAAACCCCTATTCCGACCTCCTCGACTTCCGCGCCAAGCGCGCCGTCGAGCTCGGCGCCGGATGCGGAGTCGCCGCCATGGGGCTTTACCTCCTCGGCCTGACCGACGTCGTCATCACCGACATCCCCGCCGTGATGCCGGCGCTGAAGCGAAACCTCAAGCGCAACAAACCGGTCCTCGGCAAGAACCTCAAGCACGCGATTCTCCACTGGAACAAGGCGGACCAGATCGCCGCGCTCAGCCCTCCGTACGACGTCGTTGTGGCGACCGATGTGGTTTATATTGAGGAGACCGTAGGACCCTTGATCTCCACCATGGAGGCGATGGTGAAGGACGACGGCGTCGTTTTGCTTGGGTACCAATTGAGGTCGCCGGAGGCGCATAAGCTCTTCTGGGAAATGTGTGAAGGGGTTTTTGTGATCGAGAAAGTTCCGCACCAGGATTTGCACCCGGATTATGCCTATGAGGAGACCGATGTCTATATCTTGCGCAAGAAGAAAAAGGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

225

Amino Acids

25.16

Weight (kDa)

5.97

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 16 - 189 5.6e-26 Lysine methyltransferase
Methyltransf_23 PF13489 64 - 186 3.1e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44170
fragaria_vesca FvH4_6g26750
malus_domestica MD03G1234000.v1.1 MD11G1254400.v1.1
prunus_persica Prupe.4G174700_v2.0.a1
pyrus_communis pycom03g18060
rosa_chinensis RchiOBHm_Chr0c19g0500101
rosa_laevigata RLG00000005980 RLG00000018989 RLG00000035646
rosa_multiflora Rmu_sc0014193.1_g000003
rosa_roxburghii Rroxscaffold_2G00115700 Rroxscaffold_2G00116140
rosa_rugosa Rorug02G0277800
rosa_samantha Rh2AG328900 Rh2BG338800 Rh2CG315800 Rh2DG355500
rosa_wichuraiana Rw2G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 506
AatII GACGTC 2 cut(s) 276, 423
Acc16I TGCGCA 1 cut(s) 656
Acc65I GGTACC 1 cut(s) 522
AccB1I GGYRCC 3 cut(s) 89, 221, 522
AccB7I CCANNNNNTGG 1 cut(s) 99
AccII CGCG 5 cut(s) 42, 198, 207, 365, 401
AciI CCGC 8 cut(s) 40, 196, 231, 240, 294, 386, 399, 599
AcuI CTGAAG 1 cut(s) 332
AcyI GRCGYC 5 cut(s) 45, 222, 273, 420, 507
AfaI GTAC 2 cut(s) 416, 524
AfeI AGCGCT 1 cut(s) 137
AfiI CCNNNNNNNGG 4 cut(s) 99, 138, 246, 344
AgeI ACCGGT 1 cut(s) 337
AjnI CCWGG 1 cut(s) 603
AluBI AGCT 3 cut(s) 31, 217, 553
AluI AGCT 3 cut(s) 31, 217, 553
Alw21I GWGCWC 2 cut(s) 33, 219
Alw26I GTCTC 2 cut(s) 449, 629
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 3 cut(s) 101, 141, 262
ApaI GGGCCC 1 cut(s) 145
AsiGI ACCGGT 1 cut(s) 337
Asp718I GGTACC 1 cut(s) 522
AspS9I GGNCC 6 cut(s) 102, 141, 142, 340, 388, 464
AsuC2I CCSGG 1 cut(s) 617
AsuHPI GGTGA 3 cut(s) 34, 274, 505
AvaII GGWCC 3 cut(s) 340, 388, 464
BaeGI GKGCMC 1 cut(s) 145
BanI GGYRCC 3 cut(s) 89, 221, 522
BanII GRGCYC 3 cut(s) 33, 145, 219
Bbv12I GWGCWC 2 cut(s) 33, 219
BccI CCATC 1 cut(s) 484
BceAI ACGGC 5 cut(s) 32, 88, 194, 281, 520
BciT130I CCWGG 1 cut(s) 605
BcnI CCSGG 1 cut(s) 617
BcoDI GTCTC 2 cut(s) 449, 629
BfoI RGCGCY 3 cut(s) 139, 225, 311
BisI GCNGC 2 cut(s) 240, 399
BlpI GCTNAGC 1 cut(s) 404
BlsI GCNGC 2 cut(s) 241, 400
Bme1390I CCNGG 2 cut(s) 605, 617
Bme18I GGWCC 3 cut(s) 340, 388, 464
BmgT120I GGNCC 6 cut(s) 102, 141, 142, 340, 388, 464
BmiI GGNNCC 7 cut(s) 76, 91, 143, 144, 223, 466, 524
BmrFI CCNGG 2 cut(s) 605, 617
BmsI GCATC 2 cut(s) 218, 291
BoxI GACNNNNGTC 2 cut(s) 272, 641
Bpu1102I GCTNAGC 1 cut(s) 404
BpuEI CTTGAG 2 cut(s) 308, 341
BpuMI CCSGG 1 cut(s) 617
BsaHI GRCGYC 5 cut(s) 45, 222, 273, 420, 507
BsaI GGTCTC 2 cut(s) 449, 629
BsaJI CCNNGG 4 cut(s) 243, 259, 343, 480
BsaWI WCCGGW 1 cut(s) 337
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 138, 246, 344
Bse118I RCCGGY 3 cut(s) 19, 304, 337
Bse1I ACTGG 1 cut(s) 380
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 4 cut(s) 243, 259, 343, 480
BseGI GGATG 2 cut(s) 233, 288
BseLI CCNNNNNNNGG 4 cut(s) 99, 138, 246, 344
BseMII CTCAG 1 cut(s) 418
BseNI ACTGG 1 cut(s) 380
BsePI GCGCGC 1 cut(s) 205
BseRI GAGGAG 4 cut(s) 176, 248, 467, 647
BseSI GKGCMC 1 cut(s) 145
BseYI CCCAGC 1 cut(s) 138
Bsh1236I CGCG 5 cut(s) 42, 198, 207, 365, 401
Bsh1285I CGRYCG 1 cut(s) 435
BshFI GGCC 3 cut(s) 103, 143, 264
BshNI GGYRCC 3 cut(s) 89, 221, 522
BshTI ACCGGT 1 cut(s) 337
BsiEI CGRYCG 1 cut(s) 435
BsiHKAI GWGCWC 2 cut(s) 33, 219
BsiSI CCGG 6 cut(s) 20, 225, 305, 338, 539, 617
BsiWI CGTACG 1 cut(s) 414
BslI CCNNNNNNNGG 4 cut(s) 99, 138, 246, 344
BsmAI GTCTC 2 cut(s) 449, 629
BsnI GGCC 3 cut(s) 103, 143, 264
Bso31I GGTCTC 2 cut(s) 449, 629
Bsp120I GGGCCC 1 cut(s) 141
Bsp1286I GDGCHC 3 cut(s) 33, 145, 219
Bsp143I GATC 3 cut(s) 393, 471, 585
Bsp1720I GCTNAGC 1 cut(s) 404
Bsp19I CCATGG 2 cut(s) 243, 480
BspACI CCGC 8 cut(s) 40, 196, 231, 240, 294, 386, 399, 599
BspANI GGCC 3 cut(s) 103, 143, 264
BspCNI CTCAG 1 cut(s) 417
BspFNI CGCG 5 cut(s) 42, 198, 207, 365, 401
BspLI GGNNCC 7 cut(s) 76, 91, 143, 144, 223, 466, 524
BspQI GCTCTTC 1 cut(s) 560
BspT107I GGYRCC 3 cut(s) 89, 221, 522
BspTNI GGTCTC 2 cut(s) 449, 629
BsrFI RCCGGY 3 cut(s) 19, 304, 337
BsrI ACTGG 1 cut(s) 380
BssAI RCCGGY 3 cut(s) 19, 304, 337
BssECI CCNNGG 4 cut(s) 243, 259, 343, 480
BssHII GCGCGC 1 cut(s) 205
BssMI GATC 3 cut(s) 393, 471, 585
BssNI GRCGYC 5 cut(s) 45, 222, 273, 420, 507
BssT1I CCWWGG 2 cut(s) 243, 480
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 2 cut(s) 97, 460
Bst6I CTCTTC 1 cut(s) 560
BstACI GRCGYC 5 cut(s) 45, 222, 273, 420, 507
BstC8I GCNNGC 3 cut(s) 207, 306, 363
BstDEI CTNAG 2 cut(s) 112, 404
BstDSI CCRYGG 2 cut(s) 243, 480
BstF5I GGATG 2 cut(s) 233, 288
BstFNI CGCG 5 cut(s) 42, 198, 207, 365, 401
BstH2I RGCGCY 3 cut(s) 139, 225, 311
BstKTI GATC 3 cut(s) 396, 474, 588
BstMAI GTCTC 2 cut(s) 449, 629
BstMBI GATC 3 cut(s) 393, 471, 585
BstMCI CGRYCG 1 cut(s) 435
BstMWI GCNNNNNNNGC 4 cut(s) 204, 206, 228, 544
BstNI CCWGG 1 cut(s) 605
BstPAI GACNNNNGTC 2 cut(s) 272, 641
BstSCI CCNGG 2 cut(s) 603, 615
BstSLI GKGCMC 1 cut(s) 145
BstUI CGCG 5 cut(s) 42, 198, 207, 365, 401
BsuRI GGCC 3 cut(s) 103, 143, 264
BtgI CCRYGG 2 cut(s) 243, 480
BtgZI GCGATG 1 cut(s) 503
BtsCI GGATG 2 cut(s) 233, 288
BtsIMutI CAGTG 1 cut(s) 373
Cac8I GCNNGC 3 cut(s) 207, 306, 363
Cfr10I RCCGGY 3 cut(s) 19, 304, 337
Cfr13I GGNCC 6 cut(s) 102, 141, 142, 340, 388, 464
CseI GACGC 2 cut(s) 53, 496
Csp6I GTAC 2 cut(s) 415, 523
CspAI ACCGGT 1 cut(s) 337
CviAII CATG 3 cut(s) 83, 244, 481
CviJI RGCY 9 cut(s) 31, 75, 103, 143, 217, 250, 264, 408, 553
CviKI_1 RGCY 9 cut(s) 31, 75, 103, 143, 217, 250, 264, 408, 553
CviQI GTAC 2 cut(s) 415, 523
DdeI CTNAG 2 cut(s) 112, 404
DinI GGCGCC 1 cut(s) 223
DpnI GATC 3 cut(s) 395, 473, 587
DpnII GATC 3 cut(s) 393, 471, 585
DrdI GACNNNNNNGTC 1 cut(s) 506
DseDI GACNNNNNNGTC 1 cut(s) 506
Eam1104I CTCTTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 560
EciI GGCGGA 1 cut(s) 401
Ecl136II GAGCTC 2 cut(s) 31, 217
Eco130I CCWWGG 2 cut(s) 243, 480
Eco24I GRGCYC 3 cut(s) 33, 145, 219
Eco31I GGTCTC 2 cut(s) 449, 629
Eco47I GGWCC 3 cut(s) 340, 388, 464
Eco47III AGCGCT 1 cut(s) 137
Eco53kI GAGCTC 2 cut(s) 31, 217
Eco57I CTGAAG 1 cut(s) 332
EcoICRI GAGCTC 2 cut(s) 31, 217
EcoO109I RGGNCCY 2 cut(s) 142, 464
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 2 cut(s) 243, 480
EcoT38I GRGCYC 3 cut(s) 33, 145, 219
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 243, 480
FaeI CATG 3 cut(s) 86, 247, 484
FaiI YATR 8 cut(s) 84, 245, 447, 482, 549, 624, 630, 648
FatI CATG 3 cut(s) 82, 243, 480
FauI CCCGC 1 cut(s) 301
Fnu4HI GCNGC 2 cut(s) 240, 399
FokI GGATG 2 cut(s) 240, 275
FriOI GRGCYC 3 cut(s) 33, 145, 219
Fsp4HI GCNGC 2 cut(s) 240, 399
FspI TGCGCA 1 cut(s) 656
GluI GCNGC 2 cut(s) 240, 399
GsaI CCCAGC 1 cut(s) 142
HaeII RGCGCY 3 cut(s) 139, 225, 311
HaeIII GGCC 3 cut(s) 103, 143, 264
HapII CCGG 6 cut(s) 20, 225, 305, 338, 539, 617
HgaI GACGC 2 cut(s) 53, 496
Hin1I GRCGYC 5 cut(s) 45, 222, 273, 420, 507
Hin1II CATG 3 cut(s) 86, 247, 484
HinfI GANTC 3 cut(s) 14, 234, 367
HpaII CCGG 6 cut(s) 20, 225, 305, 338, 539, 617
HphI GGTGA 3 cut(s) 34, 274, 505
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 1 cut(s) 181
Hpy188III TCNNGA 1 cut(s) 589
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 8 cut(s) 47, 215, 275, 278, 422, 425, 506, 512
HpyAV CCTTC 3 cut(s) 67, 490, 566
HpyCH4III ACNGT 2 cut(s) 97, 460
HpyCH4IV ACGT 2 cut(s) 273, 420
HpyCH4V TGCA 1 cut(s) 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 204, 206, 228, 544
HpyF3I CTNAG 2 cut(s) 112, 404
HpySE526I ACGT 2 cut(s) 273, 420
Hsp92I GRCGYC 5 cut(s) 45, 222, 273, 420, 507
Hsp92II CATG 3 cut(s) 86, 247, 484
KasI GGCGCC 1 cut(s) 221
KpnI GGTACC 1 cut(s) 526
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Kzo9I GATC 3 cut(s) 393, 471, 585
LguI GCTCTTC 1 cut(s) 560
LmnI GCTCC 3 cut(s) 36, 80, 113
LweI GCATC 2 cut(s) 218, 291
MaeII ACGT 2 cut(s) 273, 420
MalI GATC 3 cut(s) 395, 473, 587
MboI GATC 3 cut(s) 393, 471, 585
MboII GAAGA 2 cut(s) 547, 673
MfeI CAATTG 1 cut(s) 527
MhlI GDGCHC 3 cut(s) 33, 145, 219
MluCI AATT 1 cut(s) 527
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 2 cut(s) 8, 243
MmeI TCCRAC 2 cut(s) 85, 204
MroNI GCCGGC 1 cut(s) 304
MseI TTAA 1 cut(s) 123
MspI CCGG 6 cut(s) 20, 225, 305, 338, 539, 617
MspR9I CCNGG 2 cut(s) 605, 617
MunI CAATTG 1 cut(s) 527
MvaI CCWGG 1 cut(s) 605
MvnI CGCG 5 cut(s) 42, 198, 207, 365, 401
MwoI GCNNNNNNNGC 4 cut(s) 204, 206, 228, 544
NaeI GCCGGC 1 cut(s) 306
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 1 cut(s) 617
NcoI CCATGG 2 cut(s) 243, 480
NdeII GATC 3 cut(s) 393, 471, 585
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 3 cut(s) 86, 247, 484
NlaIV GGNNCC 7 cut(s) 76, 91, 143, 144, 223, 466, 524
NmeAIII GCCGAG 4 cut(s) 157, 198, 240, 324
NsbI TGCGCA 1 cut(s) 656
PauI GCGCGC 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 560
PcsI WCGNNNNNNNCGW 4 cut(s) 93, 267, 282, 507
PdiI GCCGGC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 367
Pfl23II CGTACG 1 cut(s) 414
PflFI GACNNNGTC 3 cut(s) 47, 275, 506
PflMI CCANNNNNTGG 1 cut(s) 99
PinAI ACCGGT 1 cut(s) 337
PkrI GCNGC 2 cut(s) 241, 400
PleI GAGTC 2 cut(s) 8, 242
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 2 cut(s) 8, 242
PpuMI RGGWCCY 1 cut(s) 464
PshAI GACNNNNGTC 2 cut(s) 272, 641
Psp124BI GAGCTC 2 cut(s) 33, 219
Psp5II RGGWCCY 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 603
PspFI CCCAGC 1 cut(s) 138
PspGI CCWGG 1 cut(s) 603
PspLI CGTACG 1 cut(s) 414
PspN4I GGNNCC 7 cut(s) 76, 91, 143, 144, 223, 466, 524
PspOMI GGGCCC 1 cut(s) 141
PspPI GGNCC 6 cut(s) 102, 141, 142, 340, 388, 464
PspPPI RGGWCCY 1 cut(s) 464
PsyI GACNNNGTC 3 cut(s) 47, 275, 506
PteI GCGCGC 1 cut(s) 205
RsaI GTAC 2 cut(s) 416, 524
RsaNI GTAC 2 cut(s) 415, 523
SacI GAGCTC 2 cut(s) 33, 219
SapI GCTCTTC 1 cut(s) 560
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 2 cut(s) 240, 399
Sau3AI GATC 3 cut(s) 393, 471, 585
Sau96I GGNCC 6 cut(s) 102, 141, 142, 340, 388, 464
SchI GAGTC 2 cut(s) 8, 243
ScrFI CCNGG 2 cut(s) 605, 617
SduI GDGCHC 3 cut(s) 33, 145, 219
SfaNI GCATC 2 cut(s) 218, 291
SfoI GGCGCC 1 cut(s) 223
SgrAI CRCCGGYG 1 cut(s) 19
SinI GGWCC 3 cut(s) 340, 388, 464
SmlI CTYRAG 2 cut(s) 323, 356
SmoI CTYRAG 2 cut(s) 323, 356
Sse9I AATT 1 cut(s) 527
SsiI CCGC 8 cut(s) 40, 196, 231, 240, 294, 386, 399, 599
SspDI GGCGCC 1 cut(s) 221
SstI GAGCTC 2 cut(s) 33, 219
StyD4I CCNGG 2 cut(s) 603, 615
StyI CCWWGG 2 cut(s) 243, 480
TaaI ACNGT 2 cut(s) 97, 460
TaiI ACGT 2 cut(s) 276, 423
TaqI TCGA 3 cut(s) 189, 213, 588
TaqII GACCGA 3 cut(s) 284, 449, 653
TasI AATT 1 cut(s) 527
TauI GCSGC 2 cut(s) 242, 401
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 1 cut(s) 380
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 1 cut(s) 380
Tth111I GACNNNGTC 3 cut(s) 47, 275, 506
Van91I CCANNNNNTGG 1 cut(s) 99
VpaK11BI GGWCC 3 cut(s) 340, 388, 464
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
ZraI GACGTC 2 cut(s) 274, 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.