pycom03g18060

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012406
Physical Location & Seq
Forward (+)
19123000 .. 19123701
702 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g18060.1

Sequence Viewer

Length: 702 bp
ATGAAGTTCACCGACTCACCAGTGATAGAGCTGCCGGTGCGCGACGCCCTCCTTTCCCTCCAACAAGACAACGGCTCCTTCCACGTCGGCACCTCCGTCTGGCCCTGCTCCTTAGTCCTCGTCAAGTTCGCCGAGCGCTGGGCCCCACCCAACCCCAACAACCCCTACTCCGCCCTCCTCGACTTCCACGGCAAGCGCGCCGTCGAGCTCGGCGCCGGATGCGGGGCTGCCGGCATGGGCCTGTACCTCCTCGGCCTGACCGACCTCGTCCTCACAGATATTCCCGCGGTCATGCCCGCACTGAAGCACAACCTCAAGCGCAACAAGCCGGTCCTGGGGAAAACGATCAAGCACGCCATCGTGCACTGGAACAAGGCGGCCGATCAGGCCAAGTCCCTGAGTCCGCCGTACGACGTCGTGGTGGCGACCGACGTTGTCTACATCGAGGAGACTGTGGGCCCGCTCGTGTCCACCATGGAGGCGCTTGTTAAGGACGACGGGGTTGTTTTGCTTGGGTACCAAGTGAGGTCCCCGGAAGCACACCAACTGTTCTGGGAAATGTGTGAGAGAGTTTTTTCGATCGAGAAAGTTCCTCATGAGGATTTGCACCCGAATTATGCGTACGAAGAGGCCAATGTGTTCGTCTTGCGCAAGAAACAAAGAGGAGAACTCGCTCATCAGATTAGTCCTCTTGAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

234

Amino Acids

25.77

Weight (kDa)

5.98

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 17 - 188 9.7e-27 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44170
fragaria_vesca FvH4_6g26750
malus_domestica MD03G1234000.v1.1 MD11G1254400.v1.1
prunus_persica Prupe.4G174700_v2.0.a1
pyrus_communis pycom03g18060
rosa_chinensis RchiOBHm_Chr0c19g0500101
rosa_laevigata RLG00000005980 RLG00000018989 RLG00000035646
rosa_multiflora Rmu_sc0014193.1_g000003
rosa_roxburghii Rroxscaffold_2G00115700 Rroxscaffold_2G00116140
rosa_rugosa Rorug02G0277800
rosa_samantha Rh2AG328900 Rh2BG338800 Rh2CG315800 Rh2DG355500
rosa_wichuraiana Rw2G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 417
Acc16I TGCGCA 1 cut(s) 650
Acc65I GGTACC 1 cut(s) 516
AccB1I GGYRCC 3 cut(s) 89, 212, 516
AccBSI CCGCTC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 438
AccII CGCG 3 cut(s) 42, 198, 287
AciI CCGC 8 cut(s) 171, 222, 285, 287, 297, 377, 404, 461
AcoI YGGCCR 1 cut(s) 378
AcuI CTGAAG 1 cut(s) 323
AcyI GRCGYC 3 cut(s) 45, 213, 414
AfaI GTAC 4 cut(s) 245, 410, 518, 623
AfeI AGCGCT 1 cut(s) 137
AfiI CCNNNNNNNGG 4 cut(s) 99, 138, 222, 335
AgsI TTSAA 1 cut(s) 695
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 2 cut(s) 31, 208
AluI AGCT 2 cut(s) 31, 208
Alw21I GWGCWC 2 cut(s) 210, 366
Alw26I GTCTC 1 cut(s) 443
Alw44I GTGCAC 1 cut(s) 362
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 8 cut(s) 101, 141, 238, 253, 378, 387, 457, 630
ApaI GGGCCC 2 cut(s) 145, 461
ApaLI GTGCAC 1 cut(s) 362
ApeKI GCWGC 2 cut(s) 31, 227
Asp718I GGTACC 1 cut(s) 516
AspLEI GCGC 8 cut(s) 42, 138, 198, 200, 215, 321, 484, 651
AspS9I GGNCC 8 cut(s) 102, 141, 142, 238, 331, 457, 458, 528
AsuC2I CCSGG 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 9
AvaII GGWCC 2 cut(s) 331, 528
BaeGI GKGCMC 3 cut(s) 145, 366, 461
BanI GGYRCC 3 cut(s) 89, 212, 516
BanII GRGCYC 3 cut(s) 145, 210, 461
BauI CACGAG 1 cut(s) 464
Bbv12I GWGCWC 2 cut(s) 210, 366
BbvI GCAGC 2 cut(s) 18, 214
BccI CCATC 1 cut(s) 365
BceAI ACGGC 4 cut(s) 88, 185, 205, 391
BciT130I CCWGG 1 cut(s) 335
BcnI CCSGG 1 cut(s) 533
BcoDI GTCTC 1 cut(s) 443
BfoI RGCGCY 3 cut(s) 139, 216, 485
BglI GCCNNNNNGGC 1 cut(s) 386
BisI GCNGC 3 cut(s) 32, 228, 378
BlsI GCNGC 3 cut(s) 33, 229, 379
Bme1390I CCNGG 2 cut(s) 335, 533
Bme18I GGWCC 2 cut(s) 331, 528
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 8 cut(s) 102, 141, 142, 238, 331, 457, 458, 528
BmiI GGNNCC 8 cut(s) 76, 91, 143, 144, 214, 459, 518, 530
BmrFI CCNGG 2 cut(s) 335, 533
BmsI GCATC 1 cut(s) 209
BplI GAGNNNNNCTC 2 cut(s) 654, 686
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 533
BsaHI GRCGYC 3 cut(s) 45, 213, 414
BsaJI CCNNGG 6 cut(s) 187, 250, 285, 334, 474, 531
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 152, 182
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 138, 222, 335
Bse118I RCCGGY 3 cut(s) 34, 230, 328
Bse1I ACTGG 2 cut(s) 20, 371
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 6 cut(s) 187, 250, 285, 334, 474, 531
BseGI GGATG 1 cut(s) 224
BseLI CCNNNNNNNGG 4 cut(s) 99, 138, 222, 335
BseMII CTCAG 1 cut(s) 389
BseNI ACTGG 2 cut(s) 20, 371
BsePI GCGCGC 1 cut(s) 196
BseRI GAGGAG 4 cut(s) 167, 239, 461, 678
BseSI GKGCMC 3 cut(s) 145, 366, 461
BseX3I CGGCCG 1 cut(s) 378
BseXI GCAGC 2 cut(s) 18, 214
BseYI CCCAGC 1 cut(s) 138
Bsh1236I CGCG 3 cut(s) 42, 198, 287
Bsh1285I CGRYCG 3 cut(s) 381, 429, 582
BshFI GGCC 8 cut(s) 103, 143, 240, 255, 380, 389, 459, 632
BshNI GGYRCC 3 cut(s) 89, 212, 516
BsiEI CGRYCG 3 cut(s) 381, 429, 582
BsiHKAI GWGCWC 2 cut(s) 210, 366
BsiSI CCGG 5 cut(s) 35, 216, 231, 329, 533
BsiWI CGTACG 2 cut(s) 408, 621
BslFI GGGAC 2 cut(s) 379, 514
BslI CCNNNNNNNGG 4 cut(s) 99, 138, 222, 335
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 2 cut(s) 379, 514
BsnI GGCC 8 cut(s) 103, 143, 240, 255, 380, 389, 459, 632
Bsp120I GGGCCC 2 cut(s) 141, 457
Bsp1286I GDGCHC 4 cut(s) 145, 210, 366, 461
Bsp143I GATC 3 cut(s) 345, 382, 579
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 8 cut(s) 171, 222, 285, 287, 297, 377, 404, 461
BspANI GGCC 8 cut(s) 103, 143, 240, 255, 380, 389, 459, 632
BspCNI CTCAG 1 cut(s) 390
BspFNI CGCG 3 cut(s) 42, 198, 287
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 8 cut(s) 76, 91, 143, 144, 214, 459, 518, 530
BspT107I GGYRCC 3 cut(s) 89, 212, 516
BsrBI CCGCTC 1 cut(s) 463
BsrFI RCCGGY 3 cut(s) 34, 230, 328
BsrI ACTGG 2 cut(s) 20, 371
BssAI RCCGGY 3 cut(s) 34, 230, 328
BssECI CCNNGG 6 cut(s) 187, 250, 285, 334, 474, 531
BssHII GCGCGC 1 cut(s) 196
BssMI GATC 3 cut(s) 345, 382, 579
BssNI GRCGYC 3 cut(s) 45, 213, 414
BssSI CACGAG 1 cut(s) 464
BssT1I CCWWGG 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 464
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 2 cut(s) 454, 549
Bst6I CTCTTC 1 cut(s) 621
BstACI GRCGYC 3 cut(s) 45, 213, 414
BstC8I GCNNGC 6 cut(s) 194, 198, 232, 297, 354, 461
BstDEI CTNAG 2 cut(s) 112, 398
BstDSI CCRYGG 3 cut(s) 187, 285, 474
BstF5I GGATG 1 cut(s) 224
BstFNI CGCG 3 cut(s) 42, 198, 287
BstH2I RGCGCY 3 cut(s) 139, 216, 485
BstHHI GCGC 8 cut(s) 42, 138, 198, 200, 215, 321, 484, 651
BstKTI GATC 3 cut(s) 348, 385, 582
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 3 cut(s) 345, 382, 579
BstMCI CGRYCG 3 cut(s) 381, 429, 582
BstMWI GCNNNNNNNGC 4 cut(s) 37, 219, 325, 386
BstNI CCWGG 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 333, 531
BstSLI GKGCMC 3 cut(s) 145, 366, 461
BstUI CGCG 3 cut(s) 42, 198, 287
BstV1I GCAGC 2 cut(s) 18, 214
BstZI CGGCCG 1 cut(s) 378
BsuRI GGCC 8 cut(s) 103, 143, 240, 255, 380, 389, 459, 632
BtgI CCRYGG 3 cut(s) 187, 285, 474
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 224
BtsIMutI CAGTG 3 cut(s) 27, 299, 364
Cac8I GCNNGC 6 cut(s) 194, 198, 232, 297, 354, 461
CciI TCATGA 1 cut(s) 595
CfoI GCGC 8 cut(s) 42, 138, 198, 200, 215, 321, 484, 651
Cfr10I RCCGGY 3 cut(s) 34, 230, 328
Cfr13I GGNCC 8 cut(s) 102, 141, 142, 238, 331, 457, 458, 528
Cfr42I CCGCGG 1 cut(s) 288
CseI GACGC 1 cut(s) 53
Csp6I GTAC 4 cut(s) 244, 409, 517, 622
CviAII CATG 4 cut(s) 235, 292, 475, 596
CviQI GTAC 4 cut(s) 244, 409, 517, 622
DdeI CTNAG 2 cut(s) 112, 398
DinI GGCGCC 1 cut(s) 214
DpnI GATC 3 cut(s) 347, 384, 581
DpnII GATC 3 cut(s) 345, 382, 579
EaeI YGGCCR 1 cut(s) 378
EagI CGGCCG 1 cut(s) 378
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
EciI GGCGGA 2 cut(s) 160, 393
Ecl136II GAGCTC 1 cut(s) 208
EclXI CGGCCG 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 474
Eco24I GRGCYC 3 cut(s) 145, 210, 461
Eco47I GGWCC 2 cut(s) 331, 528
Eco47III AGCGCT 1 cut(s) 137
Eco52I CGGCCG 1 cut(s) 378
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 323
EcoICRI GAGCTC 1 cut(s) 208
EcoO109I RGGNCCY 2 cut(s) 142, 528
EcoRII CCWGG 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 474
EcoT38I GRGCYC 3 cut(s) 145, 210, 461
EgeI GGCGCC 1 cut(s) 214
EheI GGCGCC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 4 cut(s) 238, 295, 478, 599
FaiI YATR 5 cut(s) 236, 293, 476, 597, 618
FaqI GGGAC 2 cut(s) 379, 514
FatI CATG 4 cut(s) 234, 291, 474, 595
FauI CCCGC 4 cut(s) 215, 292, 304, 468
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 32, 228, 378
FokI GGATG 1 cut(s) 231
FriOI GRGCYC 3 cut(s) 145, 210, 461
Fsp4HI GCNGC 3 cut(s) 32, 228, 378
FspI TGCGCA 1 cut(s) 650
GlaI GCGC 8 cut(s) 41, 137, 197, 199, 214, 320, 483, 650
GluI GCNGC 3 cut(s) 32, 228, 378
GsaI CCCAGC 1 cut(s) 142
HaeII RGCGCY 3 cut(s) 139, 216, 485
HaeIII GGCC 8 cut(s) 103, 143, 240, 255, 380, 389, 459, 632
HapII CCGG 5 cut(s) 35, 216, 231, 329, 533
HgaI GACGC 1 cut(s) 53
HhaI GCGC 8 cut(s) 42, 138, 198, 200, 215, 321, 484, 651
Hin1I GRCGYC 3 cut(s) 45, 213, 414
Hin1II CATG 4 cut(s) 238, 295, 478, 599
Hin6I GCGC 8 cut(s) 40, 136, 196, 198, 213, 319, 482, 649
HinP1I GCGC 8 cut(s) 40, 136, 196, 198, 213, 319, 482, 649
HinfI GANTC 3 cut(s) 14, 400, 695
HpaII CCGG 5 cut(s) 35, 216, 231, 329, 533
HphI GGTGA 1 cut(s) 9
Hpy166II GTNNAC 4 cut(s) 9, 364, 439, 471
Hpy188I TCNGA 1 cut(s) 681
Hpy188III TCNNGA 4 cut(s) 583, 596, 692, 699
Hpy8I GTNNAC 4 cut(s) 9, 364, 439, 471
Hpy99I CGWCG 7 cut(s) 47, 89, 206, 416, 419, 434, 500
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 2 cut(s) 454, 549
HpyCH4IV ACGT 3 cut(s) 84, 414, 432
HpyCH4V TGCA 2 cut(s) 364, 607
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 219, 325, 386
HpyF3I CTNAG 2 cut(s) 112, 398
HpySE526I ACGT 3 cut(s) 84, 414, 432
Hsp92I GRCGYC 3 cut(s) 45, 213, 414
Hsp92II CATG 4 cut(s) 238, 295, 478, 599
HspAI GCGC 8 cut(s) 40, 136, 196, 198, 213, 319, 482, 649
KasI GGCGCC 1 cut(s) 212
KpnI GGTACC 1 cut(s) 520
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
KspI CCGCGG 1 cut(s) 288
Kzo9I GATC 3 cut(s) 345, 382, 579
LmnI GCTCC 2 cut(s) 80, 113
Lsp1109I GCAGC 2 cut(s) 18, 214
LweI GCATC 1 cut(s) 209
MaeII ACGT 3 cut(s) 84, 414, 432
MalI GATC 3 cut(s) 347, 384, 581
MbiI CCGCTC 1 cut(s) 463
MboI GATC 3 cut(s) 345, 382, 579
MboII GAAGA 1 cut(s) 638
MhlI GDGCHC 4 cut(s) 145, 210, 366, 461
MluCI AATT 1 cut(s) 613
Mly113I GGCGCC 1 cut(s) 213
MlyI GAGTC 2 cut(s) 8, 409
MmeI TCCRAC 1 cut(s) 85
MroNI GCCGGC 1 cut(s) 230
MseI TTAA 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 5 cut(s) 35, 216, 231, 329, 533
MspR9I CCNGG 2 cut(s) 335, 533
MvaI CCWGG 1 cut(s) 335
MvnI CGCG 3 cut(s) 42, 198, 287
MwoI GCNNNNNNNGC 4 cut(s) 37, 219, 325, 386
NaeI GCCGGC 1 cut(s) 232
NarI GGCGCC 1 cut(s) 213
NciI CCSGG 1 cut(s) 533
NcoI CCATGG 1 cut(s) 474
NdeII GATC 3 cut(s) 345, 382, 579
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 4 cut(s) 238, 295, 478, 599
NlaIV GGNNCC 8 cut(s) 76, 91, 143, 144, 214, 459, 518, 530
NmeAIII GCCGAG 3 cut(s) 157, 189, 231
NsbI TGCGCA 1 cut(s) 650
PagI TCATGA 1 cut(s) 595
PauI GCGCGC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 258
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 1 cut(s) 695
Pfl23II CGTACG 2 cut(s) 408, 621
PflFI GACNNNGTC 2 cut(s) 266, 434
PkrI GCNGC 3 cut(s) 33, 229, 379
Ple19I CGATCG 1 cut(s) 582
PleI GAGTC 2 cut(s) 8, 408
PluTI GGCGCC 1 cut(s) 216
PpsI GAGTC 2 cut(s) 8, 408
PpuMI RGGWCCY 1 cut(s) 528
Psp124BI GAGCTC 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 528
Psp6I CCWGG 1 cut(s) 333
PspFI CCCAGC 1 cut(s) 138
PspGI CCWGG 1 cut(s) 333
PspLI CGTACG 2 cut(s) 408, 621
PspN4I GGNNCC 8 cut(s) 76, 91, 143, 144, 214, 459, 518, 530
PspOMI GGGCCC 2 cut(s) 141, 457
PspPI GGNCC 8 cut(s) 102, 141, 142, 238, 331, 457, 458, 528
PspPPI RGGWCCY 1 cut(s) 528
PsyI GACNNNGTC 2 cut(s) 266, 434
PteI GCGCGC 1 cut(s) 196
PvuI CGATCG 1 cut(s) 582
RsaI GTAC 4 cut(s) 245, 410, 518, 623
RsaNI GTAC 4 cut(s) 244, 409, 517, 622
SacI GAGCTC 1 cut(s) 210
SacII CCGCGG 1 cut(s) 288
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 3 cut(s) 32, 228, 378
Sau3AI GATC 3 cut(s) 345, 382, 579
Sau96I GGNCC 8 cut(s) 102, 141, 142, 238, 331, 457, 458, 528
SchI GAGTC 2 cut(s) 8, 409
ScrFI CCNGG 2 cut(s) 335, 533
SduI GDGCHC 4 cut(s) 145, 210, 366, 461
SfaNI GCATC 1 cut(s) 209
SfiI GGCCNNNNNGGCC 1 cut(s) 386
SfoI GGCGCC 1 cut(s) 214
Sfr303I CCGCGG 1 cut(s) 288
SgrBI CCGCGG 1 cut(s) 288
SinI GGWCC 2 cut(s) 331, 528
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 1 cut(s) 613
SsiI CCGC 8 cut(s) 171, 222, 285, 287, 297, 377, 404, 461
SspDI GGCGCC 1 cut(s) 212
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 2 cut(s) 333, 531
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 2 cut(s) 454, 549
TaiI ACGT 3 cut(s) 87, 417, 435
TaqI TCGA 5 cut(s) 180, 204, 444, 578, 582
TaqII GACCGA 2 cut(s) 275, 443
TasI AATT 1 cut(s) 613
TauI GCSGC 1 cut(s) 380
TfiI GAWTC 1 cut(s) 695
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 3 cut(s) 27, 306, 371
TseI GCWGC 2 cut(s) 31, 227
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 3 cut(s) 27, 306, 371
Tth111I GACNNNGTC 2 cut(s) 266, 434
VneI GTGCAC 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 331, 528
XmiI GTMKAC 1 cut(s) 438
ZraI GACGTC 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.