MD11G1254400.v1.1

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012406
Physical Location & Seq
Forward (+)
36728170 .. 36728622
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1254400.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGGCCTGTACCTCCTTGGCCTGACCGACCTCGTCCGCGGTCATGCCCGCACTGAAGCACAACCTCAAGCGCAACAAGCTGTCCTGGGGAAAACGATCAAGCACGCCATCGTGCACTGGAACAAGCCGGCCGATCAGGCCAAGCCCCTGAGTCCTCCGTACGACGTCGTGATGGCGACCGACGTTGTGTACATCGAAGAGACTGTGGGCCCGCTTGTGTCCACCATGGAGGCGCTGGTTAAGGACGATGGAGTTGTTTTACTTGGGTACCAAGTGAGGTCCCTGGAAGCACACCAACTGTTCTGGGAAATGAGTGAGAGAGTTTTTTTGATCGAGAAAGATCCGCATGAGGATTTGCACCCCAATTATGCGTACGAAGAGGCCGATGTGTTCGTCTTGCGCAAGAAGCAAGAAGGAGGACTCTCTCATCAGATTACTCCTCCTGAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

151

Amino Acids

16.84

Weight (kDa)

5.14

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 23 - 105 2.6e-09 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44170
fragaria_vesca FvH4_6g26750
malus_domestica MD03G1234000.v1.1 MD11G1254400.v1.1
prunus_persica Prupe.4G174700_v2.0.a1
pyrus_communis pycom03g18060
rosa_chinensis RchiOBHm_Chr0c19g0500101
rosa_laevigata RLG00000005980 RLG00000018989 RLG00000035646
rosa_multiflora Rmu_sc0014193.1_g000003
rosa_roxburghii Rroxscaffold_2G00115700 Rroxscaffold_2G00116140
rosa_rugosa Rorug02G0277800
rosa_samantha Rh2AG328900 Rh2BG338800 Rh2CG315800 Rh2DG355500
rosa_wichuraiana Rw2G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
Acc16I TGCGCA 1 cut(s) 401
Acc65I GGTACC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 267
AccII CGCG 1 cut(s) 39
AciI CCGC 5 cut(s) 37, 39, 49, 212, 344
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 75
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 5 cut(s) 11, 161, 191, 269, 374
AjnI CCWGG 2 cut(s) 84, 282
AluBI AGCT 1 cut(s) 80
AluI AGCT 1 cut(s) 80
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 194
Alw44I GTGCAC 1 cut(s) 113
AlwI GGATC 1 cut(s) 335
AoxI GGCC 6 cut(s) 4, 19, 129, 138, 208, 381
ApaI GGGCCC 1 cut(s) 212
ApaLI GTGCAC 1 cut(s) 113
Asp718I GGTACC 1 cut(s) 267
AspLEI GCGC 3 cut(s) 73, 235, 402
AspS9I GGNCC 4 cut(s) 4, 208, 209, 279
AvaII GGWCC 1 cut(s) 279
BaeGI GKGCMC 2 cut(s) 117, 212
BaeI ACNNNNGTAYC 2 cut(s) 251, 284
BanI GGYRCC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 117
BccI CCATC 3 cut(s) 116, 166, 242
BciT130I CCWGG 2 cut(s) 86, 284
BcoDI GTCTC 1 cut(s) 194
BfoI RGCGCY 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 137
Bme1390I CCNGG 2 cut(s) 86, 284
Bme18I GGWCC 1 cut(s) 279
BmgT120I GGNCC 4 cut(s) 4, 208, 209, 279
BmiI GGNNCC 3 cut(s) 210, 269, 281
BmrFI CCNGG 2 cut(s) 86, 284
BpuEI CTTGAG 1 cut(s) 51
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 5 cut(s) 16, 37, 85, 225, 282
Bse118I RCCGGY 1 cut(s) 127
Bse1I ACTGG 1 cut(s) 122
BseBI CCWGG 2 cut(s) 86, 284
BseDI CCNNGG 5 cut(s) 16, 37, 85, 225, 282
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 429
BseSI GKGCMC 2 cut(s) 117, 212
BseX3I CGGCCG 1 cut(s) 129
Bsh1236I CGCG 1 cut(s) 39
Bsh1285I CGRYCG 2 cut(s) 132, 180
BshFI GGCC 6 cut(s) 6, 21, 131, 140, 210, 383
BshNI GGYRCC 1 cut(s) 267
BsiEI CGRYCG 2 cut(s) 132, 180
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 1 cut(s) 128
BsiWI CGTACG 2 cut(s) 159, 372
BslFI GGGAC 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 6 cut(s) 6, 21, 131, 140, 210, 383
Bsp120I GGGCCC 1 cut(s) 208
Bsp1286I GDGCHC 2 cut(s) 117, 212
Bsp1407I TGTACA 1 cut(s) 189
Bsp143I GATC 4 cut(s) 96, 133, 330, 340
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 5 cut(s) 37, 39, 49, 212, 344
BspANI GGCC 6 cut(s) 6, 21, 131, 140, 210, 383
BspCNI CTCAG 1 cut(s) 141
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 3 cut(s) 210, 269, 281
BspPI GGATC 1 cut(s) 335
BspT107I GGYRCC 1 cut(s) 267
BsrFI RCCGGY 1 cut(s) 127
BsrGI TGTACA 1 cut(s) 189
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 127
BssECI CCNNGG 5 cut(s) 16, 37, 85, 225, 282
BssMI GATC 4 cut(s) 96, 133, 330, 340
BssNI GRCGYC 1 cut(s) 165
BssT1I CCWWGG 2 cut(s) 16, 225
Bst2UI CCWGG 2 cut(s) 86, 284
Bst4CI ACNGT 2 cut(s) 205, 300
Bst6I CTCTTC 2 cut(s) 192, 372
BstACI GRCGYC 1 cut(s) 165
BstAUI TGTACA 1 cut(s) 189
BstC8I GCNNGC 4 cut(s) 49, 105, 129, 212
BstDEI CTNAG 1 cut(s) 149
BstDSI CCRYGG 2 cut(s) 37, 225
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 236
BstHHI GCGC 3 cut(s) 73, 235, 402
BstKTI GATC 4 cut(s) 99, 136, 333, 343
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 4 cut(s) 96, 133, 330, 340
BstMCI CGRYCG 2 cut(s) 132, 180
BstMWI GCNNNNNNNGC 3 cut(s) 77, 137, 406
BstNI CCWGG 2 cut(s) 86, 284
BstSCI CCNGG 2 cut(s) 84, 282
BstSLI GKGCMC 2 cut(s) 117, 212
BstUI CGCG 1 cut(s) 39
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 6 cut(s) 6, 21, 131, 140, 210, 383
BtgI CCRYGG 2 cut(s) 37, 225
BtsIMutI CAGTG 2 cut(s) 51, 115
Cac8I GCNNGC 4 cut(s) 49, 105, 129, 212
CfoI GCGC 3 cut(s) 73, 235, 402
Cfr10I RCCGGY 1 cut(s) 127
Cfr13I GGNCC 4 cut(s) 4, 208, 209, 279
Cfr42I CCGCGG 1 cut(s) 40
Csp6I GTAC 5 cut(s) 10, 160, 190, 268, 373
CviAII CATG 3 cut(s) 44, 226, 347
CviJI RGCY 9 cut(s) 6, 21, 80, 127, 131, 140, 145, 210, 383
CviKI_1 RGCY 9 cut(s) 6, 21, 80, 127, 131, 140, 145, 210, 383
CviQI GTAC 5 cut(s) 10, 160, 190, 268, 373
DdeI CTNAG 1 cut(s) 149
DpnI GATC 4 cut(s) 98, 135, 332, 342
DpnII GATC 4 cut(s) 96, 133, 330, 340
EaeI YGGCCR 1 cut(s) 129
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 192, 372
EarI CTCTTC 2 cut(s) 192, 372
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 16, 225
Eco24I GRGCYC 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 279
Eco52I CGGCCG 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 75
EcoO109I RGGNCCY 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 84, 282
EcoT14I CCWWGG 2 cut(s) 16, 225
EcoT38I GRGCYC 1 cut(s) 212
ErhI CCWWGG 2 cut(s) 16, 225
FaeI CATG 3 cut(s) 47, 229, 350
FaiI YATR 4 cut(s) 45, 227, 348, 369
FaqI GGGAC 1 cut(s) 265
FatI CATG 3 cut(s) 43, 225, 346
FauI CCCGC 2 cut(s) 56, 219
FriOI GRGCYC 1 cut(s) 212
FspI TGCGCA 1 cut(s) 401
GlaI GCGC 3 cut(s) 72, 234, 401
HaeII RGCGCY 1 cut(s) 236
HaeIII GGCC 6 cut(s) 6, 21, 131, 140, 210, 383
HapII CCGG 1 cut(s) 128
HhaI GCGC 3 cut(s) 73, 235, 402
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 3 cut(s) 47, 229, 350
Hin6I GCGC 3 cut(s) 71, 233, 400
HinP1I GCGC 3 cut(s) 71, 233, 400
HinfI GANTC 3 cut(s) 151, 420, 446
HpaII CCGG 1 cut(s) 128
Hpy166II GTNNAC 3 cut(s) 115, 190, 222
Hpy188I TCNGA 1 cut(s) 432
Hpy188III TCNNGA 4 cut(s) 169, 334, 443, 450
Hpy8I GTNNAC 3 cut(s) 115, 190, 222
Hpy99I CGWCG 3 cut(s) 167, 170, 185
HpyAV CCTTC 1 cut(s) 407
HpyCH4III ACNGT 2 cut(s) 205, 300
HpyCH4IV ACGT 2 cut(s) 165, 183
HpyCH4V TGCA 2 cut(s) 115, 358
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 137, 406
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 2 cut(s) 165, 183
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 3 cut(s) 47, 229, 350
HspAI GCGC 3 cut(s) 71, 233, 400
KpnI GGTACC 1 cut(s) 271
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
KspI CCGCGG 1 cut(s) 40
Kzo9I GATC 4 cut(s) 96, 133, 330, 340
MaeII ACGT 2 cut(s) 165, 183
MalI GATC 4 cut(s) 98, 135, 332, 342
MboI GATC 4 cut(s) 96, 133, 330, 340
MboII GAAGA 2 cut(s) 209, 389
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 2 cut(s) 117, 212
MluCI AATT 1 cut(s) 364
MlyI GAGTC 2 cut(s) 160, 414
MroNI GCCGGC 1 cut(s) 127
MseI TTAA 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 39
MspI CCGG 1 cut(s) 128
MspR9I CCNGG 2 cut(s) 86, 284
MvaI CCWGG 2 cut(s) 86, 284
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 3 cut(s) 77, 137, 406
NaeI GCCGGC 1 cut(s) 129
NcoI CCATGG 1 cut(s) 225
NdeII GATC 4 cut(s) 96, 133, 330, 340
NgoMIV GCCGGC 1 cut(s) 127
NlaIII CATG 3 cut(s) 47, 229, 350
NlaIV GGNNCC 3 cut(s) 210, 269, 281
NsbI TGCGCA 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PdiI GCCGGC 1 cut(s) 129
PfeI GAWTC 1 cut(s) 446
Pfl23II CGTACG 2 cut(s) 159, 372
PflFI GACNNNGTC 1 cut(s) 32
PleI GAGTC 2 cut(s) 159, 414
PpsI GAGTC 2 cut(s) 159, 414
PpuMI RGGWCCY 1 cut(s) 279
Psp5II RGGWCCY 1 cut(s) 279
Psp6I CCWGG 2 cut(s) 84, 282
PspGI CCWGG 2 cut(s) 84, 282
PspLI CGTACG 2 cut(s) 159, 372
PspN4I GGNNCC 3 cut(s) 210, 269, 281
PspOMI GGGCCC 1 cut(s) 208
PspPI GGNCC 4 cut(s) 4, 208, 209, 279
PspPPI RGGWCCY 1 cut(s) 279
PsuI RGATCY 1 cut(s) 340
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 5 cut(s) 11, 161, 191, 269, 374
RsaNI GTAC 5 cut(s) 10, 160, 190, 268, 373
SacII CCGCGG 1 cut(s) 40
SaqAI TTAA 1 cut(s) 240
Sau3AI GATC 4 cut(s) 96, 133, 330, 340
Sau96I GGNCC 4 cut(s) 4, 208, 209, 279
SchI GAGTC 2 cut(s) 160, 414
ScrFI CCNGG 2 cut(s) 86, 284
SduI GDGCHC 2 cut(s) 117, 212
SetI ASST 7 cut(s) 15, 33, 67, 82, 168, 186, 281
SfiI GGCCNNNNNGGCC 1 cut(s) 137
Sfr303I CCGCGG 1 cut(s) 40
SgrBI CCGCGG 1 cut(s) 40
SinI GGWCC 1 cut(s) 279
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 1 cut(s) 364
SsiI CCGC 5 cut(s) 37, 39, 49, 212, 344
StyD4I CCNGG 2 cut(s) 84, 282
StyI CCWWGG 2 cut(s) 16, 225
TaaI ACNGT 2 cut(s) 205, 300
TaiI ACGT 2 cut(s) 168, 186
TaqI TCGA 2 cut(s) 195, 333
TaqII GACCGA 2 cut(s) 41, 194
TasI AATT 1 cut(s) 364
TatI WGTACW 1 cut(s) 189
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 2 cut(s) 58, 122
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 2 cut(s) 58, 122
Tth111I GACNNNGTC 1 cut(s) 32
VneI GTGCAC 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.