Prupe.4G174700_v2.0.a1

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012406
Physical Location & Seq
Reverse (-)
10340512 .. 10341222
711 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G174700.1

Sequence Viewer

Length: 669 bp
ATGAAGTTCACCGACTCACCCGTGATAGAGCTCCCGGTTCGAACCGCCGTCCTTTCCCTCCAACAGGACAACGGCTCCCTCCACGTCGGCACCTCCGTCTGGCCCTGTTCACTGGTTCTCGTCAAATTCGCCGAGCGCTGGGCCCCACCCAGCCCCGCTGCCCAAAACCCCTACGCCAACCTCCTCGACTTCCACGGCAAGCGAGCCGTGGAGCTCGGCGCCGGCTGCGGAGCCGCCGGCATGGGCCTCTATCTGCTTGGTTTGACAGACCTCGTCCTCACCGATATTCCCGCGGTCATGCCCGCACTGAAGCACAACCTCAAGCGCAACAAGTCCGTCCTGGGGAAGACGCTCAAGCACGCCATCGTGCACTGGAACAAGGCGGCGGATCAGGCCAAGTCCCTGAGCCCTCCGTACGACGTCGTTGTGGCGACGGACGTCGTTTACATAGAGGAGACTGTGGGCCCGCTCGTGTCCACCATGGAGGCGCTGGTGAAGGACGACGGCGTCGTTTTGCTCGGCTACCAGCTGAGGTCTCCGGAAGCACACCAACTGTTCTGGGAAATGTGTGAGAGGGATTATGCGTACGAAGAGGCTGATGTGTTTGTCTTGCGCAAGAAACAAAAAGGAGGGCTCACTCAGGATGCTGCTCCTGACTCTGCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

223

Amino Acids

24.14

Weight (kDa)

5.85

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44170
fragaria_vesca FvH4_6g26750
malus_domestica MD03G1234000.v1.1 MD11G1254400.v1.1
prunus_persica Prupe.4G174700_v2.0.a1
pyrus_communis pycom03g18060
rosa_chinensis RchiOBHm_Chr0c19g0500101
rosa_laevigata RLG00000005980 RLG00000018989 RLG00000035646
rosa_multiflora Rmu_sc0014193.1_g000003
rosa_roxburghii Rroxscaffold_2G00115700 Rroxscaffold_2G00116140
rosa_rugosa Rorug02G0277800
rosa_samantha Rh2AG328900 Rh2BG338800 Rh2CG315800 Rh2DG355500
rosa_wichuraiana Rw2G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 506
AatII GACGTC 2 cut(s) 423, 441
Acc16I TGCGCA 1 cut(s) 614
AccB1I GGYRCC 2 cut(s) 89, 218
AccBSI CCGCTC 1 cut(s) 469
AccII CGCG 1 cut(s) 293
AccIII TCCGGA 1 cut(s) 538
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 4 cut(s) 219, 420, 438, 507
AfaI GTAC 2 cut(s) 416, 587
AfeI AGCGCT 1 cut(s) 137
AfiI CCNNNNNNNGG 4 cut(s) 64, 99, 138, 342
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 3 cut(s) 31, 214, 529
AluI AGCT 3 cut(s) 31, 214, 529
Alw21I GWGCWC 3 cut(s) 33, 216, 372
Alw26I GTCTC 2 cut(s) 449, 540
Alw44I GTGCAC 1 cut(s) 368
AlwI GGATC 1 cut(s) 396
Aor13HI TCCGGA 1 cut(s) 538
Aor51HI AGCGCT 1 cut(s) 137
AoxI GGCC 5 cut(s) 101, 141, 244, 393, 463
ApaI GGGCCC 2 cut(s) 145, 467
ApaLI GTGCAC 1 cut(s) 368
ApeKI GCWGC 3 cut(s) 158, 225, 647
ApoI RAATTY 1 cut(s) 125
AspLEI GCGC 5 cut(s) 138, 221, 327, 490, 615
AspS9I GGNCC 6 cut(s) 102, 141, 142, 244, 463, 464
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 3 cut(s) 9, 271, 505
AsuII TTCGAA 1 cut(s) 40
BaeGI GKGCMC 3 cut(s) 145, 372, 467
BanI GGYRCC 2 cut(s) 89, 218
BanII GRGCYC 6 cut(s) 33, 145, 216, 410, 467, 636
BauI CACGAG 1 cut(s) 470
BbsI GAAGAC 1 cut(s) 353
Bbv12I GWGCWC 3 cut(s) 33, 216, 372
BbvCI CCTCAGC 1 cut(s) 530
BbvI GCAGC 3 cut(s) 145, 212, 634
BccI CCATC 1 cut(s) 371
BceAI ACGGC 5 cut(s) 32, 88, 191, 211, 520
BciT130I CCWGG 1 cut(s) 341
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 449, 540
BfoI RGCGCY 3 cut(s) 139, 222, 491
BisI GCNGC 5 cut(s) 159, 226, 234, 384, 648
BlsI GCNGC 5 cut(s) 160, 227, 235, 385, 649
Bme1390I CCNGG 2 cut(s) 35, 341
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 6 cut(s) 102, 141, 142, 244, 463, 464
BmiI GGNNCC 7 cut(s) 76, 91, 143, 144, 220, 232, 465
BmrFI CCNGG 2 cut(s) 35, 341
BmsI GCATC 1 cut(s) 634
BoxI GACNNNNGTC 1 cut(s) 437
BpiI GAAGAC 1 cut(s) 353
Bpu10I CCTNAGC 2 cut(s) 404, 530
Bpu14I TTCGAA 1 cut(s) 40
BpuEI CTTGAG 2 cut(s) 305, 338
BpuMI CCSGG 1 cut(s) 35
BsaHI GRCGYC 4 cut(s) 219, 420, 438, 507
BsaI GGTCTC 1 cut(s) 540
BsaJI CCNNGG 5 cut(s) 193, 207, 291, 340, 480
BsaWI WCCGGW 1 cut(s) 538
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 99, 138, 342
Bse118I RCCGGY 2 cut(s) 221, 236
Bse1I ACTGG 2 cut(s) 117, 377
BseAI TCCGGA 1 cut(s) 538
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 5 cut(s) 193, 207, 291, 340, 480
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 4 cut(s) 64, 99, 138, 342
BseMII CTCAG 3 cut(s) 395, 521, 653
BseNI ACTGG 2 cut(s) 117, 377
BseRI GAGGAG 2 cut(s) 173, 467
BseSI GKGCMC 3 cut(s) 145, 372, 467
BseXI GCAGC 3 cut(s) 145, 212, 634
BseYI CCCAGC 2 cut(s) 138, 149
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 5 cut(s) 103, 143, 246, 395, 465
BshNI GGYRCC 2 cut(s) 89, 218
BsiHKAI GWGCWC 3 cut(s) 33, 216, 372
BsiSI CCGG 4 cut(s) 35, 222, 237, 539
BsiWI CGTACG 2 cut(s) 414, 585
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 4 cut(s) 64, 99, 138, 342
BsmAI GTCTC 2 cut(s) 449, 540
BsmFI GGGAC 1 cut(s) 385
BsnI GGCC 5 cut(s) 103, 143, 246, 395, 465
Bso31I GGTCTC 1 cut(s) 540
Bsp119I TTCGAA 1 cut(s) 40
Bsp120I GGGCCC 2 cut(s) 141, 463
Bsp1286I GDGCHC 7 cut(s) 33, 145, 216, 372, 410, 467, 636
Bsp13I TCCGGA 1 cut(s) 538
Bsp143I GATC 1 cut(s) 388
Bsp19I CCATGG 1 cut(s) 480
BspANI GGCC 5 cut(s) 103, 143, 246, 395, 465
BspCNI CTCAG 3 cut(s) 396, 522, 652
BspEI TCCGGA 1 cut(s) 538
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 7 cut(s) 76, 91, 143, 144, 220, 232, 465
BspPI GGATC 1 cut(s) 396
BspT104I TTCGAA 1 cut(s) 40
BspT107I GGYRCC 2 cut(s) 89, 218
BspTNI GGTCTC 1 cut(s) 540
BsrBI CCGCTC 1 cut(s) 469
BsrFI RCCGGY 2 cut(s) 221, 236
BsrI ACTGG 2 cut(s) 117, 377
BssAI RCCGGY 2 cut(s) 221, 236
BssECI CCNNGG 5 cut(s) 193, 207, 291, 340, 480
BssMI GATC 1 cut(s) 388
BssNI GRCGYC 4 cut(s) 219, 420, 438, 507
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 1 cut(s) 480
Bst2BI CACGAG 1 cut(s) 470
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 2 cut(s) 460, 555
Bst6I CTCTTC 1 cut(s) 585
BstACI GRCGYC 4 cut(s) 219, 420, 438, 507
BstBI TTCGAA 1 cut(s) 40
BstC8I GCNNGC 7 cut(s) 200, 204, 223, 238, 303, 360, 467
BstDEI CTNAG 3 cut(s) 404, 530, 639
BstDSI CCRYGG 4 cut(s) 193, 207, 291, 480
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 1 cut(s) 649
BstFNI CGCG 1 cut(s) 293
BstH2I RGCGCY 3 cut(s) 139, 222, 491
BstHHI GCGC 5 cut(s) 138, 221, 327, 490, 615
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 2 cut(s) 449, 540
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 2 cut(s) 225, 392
BstNI CCWGG 1 cut(s) 341
BstPAI GACNNNNGTC 1 cut(s) 437
BstSCI CCNGG 2 cut(s) 33, 339
BstSLI GKGCMC 3 cut(s) 145, 372, 467
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 3 cut(s) 145, 212, 634
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 5 cut(s) 103, 143, 246, 395, 465
BtgI CCRYGG 4 cut(s) 193, 207, 291, 480
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 649
BtsIMutI CAGTG 3 cut(s) 110, 305, 370
Cac8I GCNNGC 7 cut(s) 200, 204, 223, 238, 303, 360, 467
CfoI GCGC 5 cut(s) 138, 221, 327, 490, 615
Cfr10I RCCGGY 2 cut(s) 221, 236
Cfr13I GGNCC 6 cut(s) 102, 141, 142, 244, 463, 464
Cfr42I CCGCGG 1 cut(s) 294
CseI GACGC 2 cut(s) 358, 496
Csp6I GTAC 2 cut(s) 415, 586
CviAII CATG 3 cut(s) 241, 298, 481
CviQI GTAC 2 cut(s) 415, 586
DdeI CTNAG 3 cut(s) 404, 530, 639
DinI GGCGCC 1 cut(s) 220
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
DrdI GACNNNNNNGTC 1 cut(s) 506
DseDI GACNNNNNNGTC 1 cut(s) 506
Eam1104I CTCTTC 1 cut(s) 585
EarI CTCTTC 1 cut(s) 585
EciI GGCGGA 1 cut(s) 401
Ecl136II GAGCTC 2 cut(s) 31, 214
Eco130I CCWWGG 1 cut(s) 480
Eco24I GRGCYC 6 cut(s) 33, 145, 216, 410, 467, 636
Eco31I GGTCTC 1 cut(s) 540
Eco47III AGCGCT 1 cut(s) 137
Eco53kI GAGCTC 2 cut(s) 31, 214
Eco57I CTGAAG 1 cut(s) 329
EcoICRI GAGCTC 2 cut(s) 31, 214
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 480
EcoT38I GRGCYC 6 cut(s) 33, 145, 216, 410, 467, 636
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 480
FaeI CATG 3 cut(s) 244, 301, 484
FaiI YATR 5 cut(s) 242, 299, 449, 482, 582
FaqI GGGAC 1 cut(s) 385
FatI CATG 3 cut(s) 240, 297, 480
FauI CCCGC 4 cut(s) 163, 298, 310, 474
Fnu4HI GCNGC 5 cut(s) 159, 226, 234, 384, 648
FokI GGATG 1 cut(s) 656
FriOI GRGCYC 6 cut(s) 33, 145, 216, 410, 467, 636
Fsp4HI GCNGC 5 cut(s) 159, 226, 234, 384, 648
FspI TGCGCA 1 cut(s) 614
GlaI GCGC 5 cut(s) 137, 220, 326, 489, 614
GluI GCNGC 5 cut(s) 159, 226, 234, 384, 648
GsaI CCCAGC 2 cut(s) 142, 153
HaeII RGCGCY 3 cut(s) 139, 222, 491
HaeIII GGCC 5 cut(s) 103, 143, 246, 395, 465
HapII CCGG 4 cut(s) 35, 222, 237, 539
HgaI GACGC 2 cut(s) 358, 496
HhaI GCGC 5 cut(s) 138, 221, 327, 490, 615
Hin1I GRCGYC 4 cut(s) 219, 420, 438, 507
Hin1II CATG 3 cut(s) 244, 301, 484
Hin6I GCGC 5 cut(s) 136, 219, 325, 488, 613
HinP1I GCGC 5 cut(s) 136, 219, 325, 488, 613
HinfI GANTC 2 cut(s) 14, 656
HpaII CCGG 4 cut(s) 35, 222, 237, 539
HphI GGTGA 3 cut(s) 9, 271, 505
Hpy166II GTNNAC 5 cut(s) 9, 110, 370, 445, 477
Hpy188III TCNNGA 3 cut(s) 539, 641, 653
Hpy8I GTNNAC 5 cut(s) 9, 110, 370, 445, 477
Hpy99I CGWCG 7 cut(s) 89, 422, 425, 436, 443, 506, 512
HpyAV CCTTC 1 cut(s) 490
HpyCH4III ACNGT 2 cut(s) 460, 555
HpyCH4IV ACGT 3 cut(s) 84, 420, 438
HpyCH4V TGCA 1 cut(s) 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 225, 392
HpyF3I CTNAG 3 cut(s) 404, 530, 639
HpySE526I ACGT 3 cut(s) 84, 420, 438
Hsp92I GRCGYC 4 cut(s) 219, 420, 438, 507
Hsp92II CATG 3 cut(s) 244, 301, 484
HspAI GCGC 5 cut(s) 136, 219, 325, 488, 613
KasI GGCGCC 1 cut(s) 218
Kpn2I TCCGGA 1 cut(s) 538
KroI GCCGGC 2 cut(s) 221, 236
KroNI GCCGGC 2 cut(s) 223, 238
KspI CCGCGG 1 cut(s) 294
Kzo9I GATC 1 cut(s) 388
LmnI GCTCC 5 cut(s) 36, 80, 211, 230, 655
Lsp1109I GCAGC 3 cut(s) 145, 212, 634
LweI GCATC 1 cut(s) 634
MaeII ACGT 3 cut(s) 84, 420, 438
MalI GATC 1 cut(s) 390
MbiI CCGCTC 1 cut(s) 469
MboI GATC 1 cut(s) 388
MboII GAAGA 2 cut(s) 358, 602
MhlI GDGCHC 7 cut(s) 33, 145, 216, 372, 410, 467, 636
MluCI AATT 1 cut(s) 125
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 2 cut(s) 8, 650
MmeI TCCRAC 1 cut(s) 85
MroI TCCGGA 1 cut(s) 538
MroNI GCCGGC 2 cut(s) 221, 236
MspA1I CMGCKG 3 cut(s) 158, 293, 529
MspI CCGG 4 cut(s) 35, 222, 237, 539
MspR9I CCNGG 2 cut(s) 35, 341
MvaI CCWGG 1 cut(s) 341
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 2 cut(s) 225, 392
NaeI GCCGGC 2 cut(s) 223, 238
NarI GGCGCC 1 cut(s) 219
NciI CCSGG 1 cut(s) 35
NcoI CCATGG 1 cut(s) 480
NdeII GATC 1 cut(s) 388
NgoMIV GCCGGC 2 cut(s) 221, 236
NlaIII CATG 3 cut(s) 244, 301, 484
NlaIV GGNNCC 7 cut(s) 76, 91, 143, 144, 220, 232, 465
NmeAIII GCCGAG 3 cut(s) 157, 195, 498
NsbI TGCGCA 1 cut(s) 614
NspV TTCGAA 1 cut(s) 40
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 279, 507
PdiI GCCGGC 2 cut(s) 223, 238
Pfl23II CGTACG 2 cut(s) 414, 585
PflFI GACNNNGTC 2 cut(s) 272, 506
PkrI GCNGC 5 cut(s) 160, 227, 235, 385, 649
PleI GAGTC 2 cut(s) 8, 650
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 2 cut(s) 8, 650
PshAI GACNNNNGTC 1 cut(s) 437
Psp124BI GAGCTC 2 cut(s) 33, 216
Psp6I CCWGG 1 cut(s) 339
PspFI CCCAGC 2 cut(s) 138, 149
PspGI CCWGG 1 cut(s) 339
PspLI CGTACG 2 cut(s) 414, 585
PspN4I GGNNCC 7 cut(s) 76, 91, 143, 144, 220, 232, 465
PspOMI GGGCCC 2 cut(s) 141, 463
PspPI GGNCC 6 cut(s) 102, 141, 142, 244, 463, 464
PsyI GACNNNGTC 2 cut(s) 272, 506
PvuII CAGCTG 1 cut(s) 529
RsaI GTAC 2 cut(s) 416, 587
RsaNI GTAC 2 cut(s) 415, 586
SacI GAGCTC 2 cut(s) 33, 216
SacII CCGCGG 1 cut(s) 294
SatI GCNGC 5 cut(s) 159, 226, 234, 384, 648
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 6 cut(s) 102, 141, 142, 244, 463, 464
SchI GAGTC 2 cut(s) 8, 650
ScrFI CCNGG 2 cut(s) 35, 341
SduI GDGCHC 7 cut(s) 33, 145, 216, 372, 410, 467, 636
SfaNI GCATC 1 cut(s) 634
SfoI GGCGCC 1 cut(s) 220
Sfr303I CCGCGG 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 40
SgrBI CCGCGG 1 cut(s) 294
SmlI CTYRAG 2 cut(s) 320, 353
SmoI CTYRAG 2 cut(s) 320, 353
Sse9I AATT 1 cut(s) 125
SspDI GGCGCC 1 cut(s) 218
SstI GAGCTC 2 cut(s) 33, 216
StyD4I CCNGG 2 cut(s) 33, 339
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 2 cut(s) 460, 555
TaiI ACGT 3 cut(s) 87, 423, 441
TaqI TCGA 2 cut(s) 40, 186
TasI AATT 1 cut(s) 125
TauI GCSGC 2 cut(s) 236, 386
TscAI CASTG 3 cut(s) 117, 312, 377
TseI GCWGC 3 cut(s) 158, 225, 647
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 4 cut(s) 85, 325, 402, 449
TspRI CASTG 3 cut(s) 117, 312, 377
Tth111I GACNNNGTC 2 cut(s) 272, 506
VneI GTGCAC 1 cut(s) 368
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
ZraI GACGTC 2 cut(s) 421, 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.