Rh2AG328900

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012406
Physical Location & Seq
Reverse (-)
47294225 .. 47310872
16648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG328900.1

Sequence Viewer

Length: 711 bp
ATGAATGAATCTAAACAAAACGGCGGCAAAGGTATGAAGTTCACAGACTCGCCGGTGATAGAGCTCCCCGTCCGCGACGCCGTCCTCTCCTTCCAACAAGACAACGGCTCCCTCCATGTCGGCACCACCGTCTGGCCCTGCTCCTTAGTCCTCGTCAAGTTCGCCGAGCGCTGGGCCCCACCCAGCAACCACCAACCCCAAAACCCCTATTCCGACCTCCTCGATTTCCGCGCCAAGCGCGCCGTCGAGCTCGGCGCCGGATGCGGAGTCGCCGCCATGGGGCTTTACCTCCTCGGCCTGACCGACGTCGTCATCACCGACATCCCCGCCGTGATGCCGGCGCTGAAGCGAAACCTCAAGCGCAACAAACCGGTCCTCGGCAAGAACCTCAAGCACGCGATTCTCCACTGGAACAAGGCGGACCAGATCGCCGCGCTCAGCCCTCCGTACGACGTCGTTGTGGCGACCGATGTGGTTTATATTGAGGAGACCGTGGGACCCTTGATCTCCACCATGGAGGCGATGGTGAAGGACGACGGCGTCGTTTTGCTTGGGTACCAATTGAGGTCGCCGGAGGCGCATAAGCTCTTCTGGGAAATGTGTGAAGGGGTTTTTGTGATCGAGAAAGTTCCGCACCAGGATTTGCACCCGGATTATGCGTATGAGGAGACCGATGTCTATATCTTGCGCAAGAAGAACAAGGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

236

Amino Acids

26.28

Weight (kDa)

5.98

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 27 - 200 6.6e-26 Lysine methyltransferase
Methyltransf_23 PF13489 75 - 197 3.5e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44170
fragaria_vesca FvH4_6g26750
malus_domestica MD03G1234000.v1.1 MD11G1254400.v1.1
prunus_persica Prupe.4G174700_v2.0.a1
pyrus_communis pycom03g18060
rosa_chinensis RchiOBHm_Chr0c19g0500101
rosa_laevigata RLG00000005980 RLG00000018989 RLG00000035646
rosa_multiflora Rmu_sc0014193.1_g000003
rosa_roxburghii Rroxscaffold_2G00115700 Rroxscaffold_2G00116140
rosa_rugosa Rorug02G0277800
rosa_samantha Rh2AG328900 Rh2BG338800 Rh2CG315800 Rh2DG355500
rosa_wichuraiana Rw2G026660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 539
AatII GACGTC 2 cut(s) 309, 456
Acc16I TGCGCA 1 cut(s) 689
Acc65I GGTACC 1 cut(s) 555
AccB1I GGYRCC 3 cut(s) 122, 254, 555
AccB7I CCANNNNNTGG 1 cut(s) 132
AccII CGCG 5 cut(s) 75, 231, 240, 398, 434
AciI CCGC 9 cut(s) 24, 73, 229, 264, 273, 327, 419, 432, 632
AcuI CTGAAG 1 cut(s) 365
AcyI GRCGYC 5 cut(s) 78, 255, 306, 453, 540
AfaI GTAC 2 cut(s) 449, 557
AfeI AGCGCT 1 cut(s) 170
AfiI CCNNNNNNNGG 4 cut(s) 132, 171, 279, 377
AgeI ACCGGT 1 cut(s) 370
AjnI CCWGG 1 cut(s) 636
AluBI AGCT 3 cut(s) 64, 250, 586
AluI AGCT 3 cut(s) 64, 250, 586
Alw21I GWGCWC 2 cut(s) 66, 252
Alw26I GTCTC 2 cut(s) 482, 662
Aor51HI AGCGCT 1 cut(s) 170
AoxI GGCC 3 cut(s) 134, 174, 295
ApaI GGGCCC 1 cut(s) 178
AsiGI ACCGGT 1 cut(s) 370
Asp718I GGTACC 1 cut(s) 555
AspS9I GGNCC 6 cut(s) 135, 174, 175, 373, 421, 497
AsuC2I CCSGG 1 cut(s) 650
AsuHPI GGTGA 3 cut(s) 67, 307, 538
AvaII GGWCC 3 cut(s) 373, 421, 497
BaeGI GKGCMC 1 cut(s) 178
BanI GGYRCC 3 cut(s) 122, 254, 555
BanII GRGCYC 3 cut(s) 66, 178, 252
Bbv12I GWGCWC 2 cut(s) 66, 252
BccI CCATC 1 cut(s) 517
BceAI ACGGC 6 cut(s) 37, 65, 121, 227, 314, 553
BciT130I CCWGG 1 cut(s) 638
BcnI CCSGG 1 cut(s) 650
BcoDI GTCTC 2 cut(s) 482, 662
BfoI RGCGCY 3 cut(s) 172, 258, 344
BisI GCNGC 3 cut(s) 25, 273, 432
BlpI GCTNAGC 1 cut(s) 437
BlsI GCNGC 3 cut(s) 26, 274, 433
Bme1390I CCNGG 2 cut(s) 638, 650
Bme18I GGWCC 3 cut(s) 373, 421, 497
BmgT120I GGNCC 6 cut(s) 135, 174, 175, 373, 421, 497
BmiI GGNNCC 8 cut(s) 109, 124, 176, 177, 256, 498, 499, 557
BmrFI CCNGG 2 cut(s) 638, 650
BmsI GCATC 2 cut(s) 251, 324
BoxI GACNNNNGTC 2 cut(s) 305, 674
Bpu1102I GCTNAGC 1 cut(s) 437
BpuEI CTTGAG 2 cut(s) 341, 374
BpuMI CCSGG 1 cut(s) 650
BsaHI GRCGYC 5 cut(s) 78, 255, 306, 453, 540
BsaI GGTCTC 2 cut(s) 482, 662
BsaJI CCNNGG 5 cut(s) 276, 292, 376, 492, 513
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 171, 279, 377
Bse118I RCCGGY 3 cut(s) 52, 337, 370
Bse1I ACTGG 1 cut(s) 413
BseBI CCWGG 1 cut(s) 638
BseDI CCNNGG 5 cut(s) 276, 292, 376, 492, 513
BseGI GGATG 2 cut(s) 266, 321
BseLI CCNNNNNNNGG 4 cut(s) 132, 171, 279, 377
BseMII CTCAG 1 cut(s) 451
BseNI ACTGG 1 cut(s) 413
BsePI GCGCGC 1 cut(s) 238
BseRI GAGGAG 4 cut(s) 209, 281, 500, 680
BseSI GKGCMC 1 cut(s) 178
BseYI CCCAGC 2 cut(s) 171, 182
Bsh1236I CGCG 5 cut(s) 75, 231, 240, 398, 434
Bsh1285I CGRYCG 1 cut(s) 468
BshFI GGCC 3 cut(s) 136, 176, 297
BshNI GGYRCC 3 cut(s) 122, 254, 555
BshTI ACCGGT 1 cut(s) 370
BsiEI CGRYCG 1 cut(s) 468
BsiHKAI GWGCWC 2 cut(s) 66, 252
BsiSI CCGG 6 cut(s) 53, 258, 338, 371, 572, 650
BsiWI CGTACG 1 cut(s) 447
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 4 cut(s) 132, 171, 279, 377
BsmAI GTCTC 2 cut(s) 482, 662
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 3 cut(s) 136, 176, 297
Bso31I GGTCTC 2 cut(s) 482, 662
Bsp120I GGGCCC 1 cut(s) 174
Bsp1286I GDGCHC 3 cut(s) 66, 178, 252
Bsp143I GATC 3 cut(s) 426, 504, 618
Bsp1720I GCTNAGC 1 cut(s) 437
Bsp19I CCATGG 2 cut(s) 276, 513
BspACI CCGC 9 cut(s) 24, 73, 229, 264, 273, 327, 419, 432, 632
BspANI GGCC 3 cut(s) 136, 176, 297
BspCNI CTCAG 1 cut(s) 450
BspFNI CGCG 5 cut(s) 75, 231, 240, 398, 434
BspLI GGNNCC 8 cut(s) 109, 124, 176, 177, 256, 498, 499, 557
BspQI GCTCTTC 1 cut(s) 593
BspT107I GGYRCC 3 cut(s) 122, 254, 555
BspTNI GGTCTC 2 cut(s) 482, 662
BsrFI RCCGGY 3 cut(s) 52, 337, 370
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 3 cut(s) 52, 337, 370
BssECI CCNNGG 5 cut(s) 276, 292, 376, 492, 513
BssHII GCGCGC 1 cut(s) 238
BssMI GATC 3 cut(s) 426, 504, 618
BssNI GRCGYC 5 cut(s) 78, 255, 306, 453, 540
BssT1I CCWWGG 2 cut(s) 276, 513
Bst2UI CCWGG 1 cut(s) 638
Bst4CI ACNGT 2 cut(s) 130, 493
Bst6I CTCTTC 1 cut(s) 593
BstACI GRCGYC 5 cut(s) 78, 255, 306, 453, 540
BstC8I GCNNGC 3 cut(s) 240, 339, 396
BstDEI CTNAG 2 cut(s) 145, 437
BstDSI CCRYGG 3 cut(s) 276, 492, 513
BstF5I GGATG 2 cut(s) 266, 321
BstFNI CGCG 5 cut(s) 75, 231, 240, 398, 434
BstH2I RGCGCY 3 cut(s) 172, 258, 344
BstKTI GATC 3 cut(s) 429, 507, 621
BstMAI GTCTC 2 cut(s) 482, 662
BstMBI GATC 3 cut(s) 426, 504, 618
BstMCI CGRYCG 1 cut(s) 468
BstMWI GCNNNNNNNGC 4 cut(s) 237, 239, 261, 577
BstNI CCWGG 1 cut(s) 638
BstPAI GACNNNNGTC 2 cut(s) 305, 674
BstSCI CCNGG 2 cut(s) 636, 648
BstSLI GKGCMC 1 cut(s) 178
BstUI CGCG 5 cut(s) 75, 231, 240, 398, 434
BsuRI GGCC 3 cut(s) 136, 176, 297
BtgI CCRYGG 3 cut(s) 276, 492, 513
BtgZI GCGATG 1 cut(s) 536
BtsCI GGATG 2 cut(s) 266, 321
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 3 cut(s) 240, 339, 396
Cfr10I RCCGGY 3 cut(s) 52, 337, 370
Cfr13I GGNCC 6 cut(s) 135, 174, 175, 373, 421, 497
CseI GACGC 2 cut(s) 86, 529
Csp6I GTAC 2 cut(s) 448, 556
CspAI ACCGGT 1 cut(s) 370
CviAII CATG 3 cut(s) 116, 277, 514
CviJI RGCY 9 cut(s) 64, 108, 136, 176, 250, 283, 297, 441, 586
CviKI_1 RGCY 9 cut(s) 64, 108, 136, 176, 250, 283, 297, 441, 586
CviQI GTAC 2 cut(s) 448, 556
DdeI CTNAG 2 cut(s) 145, 437
DinI GGCGCC 1 cut(s) 256
DpnI GATC 3 cut(s) 428, 506, 620
DpnII GATC 3 cut(s) 426, 504, 618
DrdI GACNNNNNNGTC 1 cut(s) 539
DseDI GACNNNNNNGTC 1 cut(s) 539
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
EciI GGCGGA 1 cut(s) 434
Ecl136II GAGCTC 2 cut(s) 64, 250
Eco130I CCWWGG 2 cut(s) 276, 513
Eco24I GRGCYC 3 cut(s) 66, 178, 252
Eco31I GGTCTC 2 cut(s) 482, 662
Eco47I GGWCC 3 cut(s) 373, 421, 497
Eco47III AGCGCT 1 cut(s) 170
Eco53kI GAGCTC 2 cut(s) 64, 250
Eco57I CTGAAG 1 cut(s) 365
EcoICRI GAGCTC 2 cut(s) 64, 250
EcoO109I RGGNCCY 2 cut(s) 175, 497
EcoRII CCWGG 1 cut(s) 636
EcoT14I CCWWGG 2 cut(s) 276, 513
EcoT38I GRGCYC 3 cut(s) 66, 178, 252
EgeI GGCGCC 1 cut(s) 256
EheI GGCGCC 1 cut(s) 256
ErhI CCWWGG 2 cut(s) 276, 513
FaeI CATG 3 cut(s) 119, 280, 517
FaiI YATR 9 cut(s) 35, 117, 278, 480, 515, 582, 657, 663, 681
FaqI GGGAC 1 cut(s) 510
FatI CATG 3 cut(s) 115, 276, 513
FauI CCCGC 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 25, 273, 432
FokI GGATG 2 cut(s) 273, 308
FriOI GRGCYC 3 cut(s) 66, 178, 252
Fsp4HI GCNGC 3 cut(s) 25, 273, 432
FspI TGCGCA 1 cut(s) 689
GluI GCNGC 3 cut(s) 25, 273, 432
GsaI CCCAGC 2 cut(s) 175, 186
HaeII RGCGCY 3 cut(s) 172, 258, 344
HaeIII GGCC 3 cut(s) 136, 176, 297
HapII CCGG 6 cut(s) 53, 258, 338, 371, 572, 650
HgaI GACGC 2 cut(s) 86, 529
Hin1I GRCGYC 5 cut(s) 78, 255, 306, 453, 540
Hin1II CATG 3 cut(s) 119, 280, 517
HinfI GANTC 4 cut(s) 8, 47, 267, 400
HpaII CCGG 6 cut(s) 53, 258, 338, 371, 572, 650
HphI GGTGA 3 cut(s) 67, 307, 538
Hpy166II GTNNAC 1 cut(s) 42
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 1 cut(s) 622
Hpy8I GTNNAC 1 cut(s) 42
Hpy99I CGWCG 8 cut(s) 80, 248, 308, 311, 455, 458, 539, 545
HpyAV CCTTC 3 cut(s) 100, 523, 599
HpyCH4III ACNGT 2 cut(s) 130, 493
HpyCH4IV ACGT 2 cut(s) 306, 453
HpyCH4V TGCA 1 cut(s) 646
HpyF10VI GCNNNNNNNGC 4 cut(s) 237, 239, 261, 577
HpyF3I CTNAG 2 cut(s) 145, 437
HpySE526I ACGT 2 cut(s) 306, 453
Hsp92I GRCGYC 5 cut(s) 78, 255, 306, 453, 540
Hsp92II CATG 3 cut(s) 119, 280, 517
KasI GGCGCC 1 cut(s) 254
KflI GGGWCCC 1 cut(s) 497
KpnI GGTACC 1 cut(s) 559
KroI GCCGGC 1 cut(s) 337
KroNI GCCGGC 1 cut(s) 339
Kzo9I GATC 3 cut(s) 426, 504, 618
LguI GCTCTTC 1 cut(s) 593
LmnI GCTCC 3 cut(s) 69, 113, 146
LweI GCATC 2 cut(s) 251, 324
MaeII ACGT 2 cut(s) 306, 453
MalI GATC 3 cut(s) 428, 506, 620
MboI GATC 3 cut(s) 426, 504, 618
MboII GAAGA 2 cut(s) 580, 706
MfeI CAATTG 1 cut(s) 560
MhlI GDGCHC 3 cut(s) 66, 178, 252
MluCI AATT 1 cut(s) 560
Mly113I GGCGCC 1 cut(s) 255
MlyI GAGTC 2 cut(s) 41, 276
MmeI TCCRAC 2 cut(s) 118, 237
MroNI GCCGGC 1 cut(s) 337
MspI CCGG 6 cut(s) 53, 258, 338, 371, 572, 650
MspR9I CCNGG 2 cut(s) 638, 650
MunI CAATTG 1 cut(s) 560
MvaI CCWGG 1 cut(s) 638
MvnI CGCG 5 cut(s) 75, 231, 240, 398, 434
MwoI GCNNNNNNNGC 4 cut(s) 237, 239, 261, 577
NaeI GCCGGC 1 cut(s) 339
NarI GGCGCC 1 cut(s) 255
NciI CCSGG 1 cut(s) 650
NcoI CCATGG 2 cut(s) 276, 513
NdeII GATC 3 cut(s) 426, 504, 618
NgoMIV GCCGGC 1 cut(s) 337
NlaIII CATG 3 cut(s) 119, 280, 517
NlaIV GGNNCC 8 cut(s) 109, 124, 176, 177, 256, 498, 499, 557
NmeAIII GCCGAG 4 cut(s) 190, 231, 273, 357
NsbI TGCGCA 1 cut(s) 689
PauI GCGCGC 1 cut(s) 238
PciSI GCTCTTC 1 cut(s) 593
PcsI WCGNNNNNNNCGW 4 cut(s) 126, 300, 315, 540
PdiI GCCGGC 1 cut(s) 339
PfeI GAWTC 2 cut(s) 8, 400
Pfl23II CGTACG 1 cut(s) 447
PflFI GACNNNGTC 3 cut(s) 80, 308, 539
PflMI CCANNNNNTGG 1 cut(s) 132
PinAI ACCGGT 1 cut(s) 370
PkrI GCNGC 3 cut(s) 26, 274, 433
PleI GAGTC 2 cut(s) 41, 275
PluTI GGCGCC 1 cut(s) 258
PpsI GAGTC 2 cut(s) 41, 275
PpuMI RGGWCCY 1 cut(s) 497
PshAI GACNNNNGTC 2 cut(s) 305, 674
Psp124BI GAGCTC 2 cut(s) 66, 252
Psp5II RGGWCCY 1 cut(s) 497
Psp6I CCWGG 1 cut(s) 636
PspFI CCCAGC 2 cut(s) 171, 182
PspGI CCWGG 1 cut(s) 636
PspLI CGTACG 1 cut(s) 447
PspN4I GGNNCC 8 cut(s) 109, 124, 176, 177, 256, 498, 499, 557
PspOMI GGGCCC 1 cut(s) 174
PspPI GGNCC 6 cut(s) 135, 174, 175, 373, 421, 497
PspPPI RGGWCCY 1 cut(s) 497
PsyI GACNNNGTC 3 cut(s) 80, 308, 539
PteI GCGCGC 1 cut(s) 238
RsaI GTAC 2 cut(s) 449, 557
RsaNI GTAC 2 cut(s) 448, 556
SacI GAGCTC 2 cut(s) 66, 252
SapI GCTCTTC 1 cut(s) 593
SatI GCNGC 3 cut(s) 25, 273, 432
Sau3AI GATC 3 cut(s) 426, 504, 618
Sau96I GGNCC 6 cut(s) 135, 174, 175, 373, 421, 497
SchI GAGTC 2 cut(s) 41, 276
ScrFI CCNGG 2 cut(s) 638, 650
SduI GDGCHC 3 cut(s) 66, 178, 252
SfaNI GCATC 2 cut(s) 251, 324
SfoI GGCGCC 1 cut(s) 256
SgrAI CRCCGGYG 1 cut(s) 52
SinI GGWCC 3 cut(s) 373, 421, 497
SmlI CTYRAG 2 cut(s) 356, 389
SmoI CTYRAG 2 cut(s) 356, 389
Sse9I AATT 1 cut(s) 560
SsiI CCGC 9 cut(s) 24, 73, 229, 264, 273, 327, 419, 432, 632
SspDI GGCGCC 1 cut(s) 254
SstI GAGCTC 2 cut(s) 66, 252
StyD4I CCNGG 2 cut(s) 636, 648
StyI CCWWGG 2 cut(s) 276, 513
TaaI ACNGT 2 cut(s) 130, 493
TaiI ACGT 2 cut(s) 309, 456
TaqI TCGA 3 cut(s) 222, 246, 621
TaqII GACCGA 3 cut(s) 317, 482, 686
TasI AATT 1 cut(s) 560
TauI GCSGC 3 cut(s) 27, 275, 434
TfiI GAWTC 2 cut(s) 8, 400
TscAI CASTG 1 cut(s) 413
TspDTI ATGAA 3 cut(s) 17, 21, 50
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 1 cut(s) 413
Tth111I GACNNNGTC 3 cut(s) 80, 308, 539
Van91I CCANNNNNTGG 1 cut(s) 132
VpaK11BI GGWCC 3 cut(s) 373, 421, 497
XcmI CCANNNNNNNNNTGG 1 cut(s) 520
ZraI GACGTC 2 cut(s) 307, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.