Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0005600

Physical Location & Seq

Genomic Coordinates Reverse (-)

20963360 .. 20969343

Gene Structure 5984 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g27390.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2886 bp

ATGGCTTCGAGGAATTTGGAGAAGATGGCTTCAATTGATGCGCAGCTGAGGCTGTTGGCACCGGGCAAGGTGTCTGAGGATGACAAACTCATCGAGTATGATGCTTTGTTGTTGGATCGCTTTCTTGACATTCTTCAGGATTTACATGGGGAGGATCTCAGAGAAACGGTTCAAGATTGTTATGAGCTATCCGCGGAATATGAAGGAAAGCAGGACCCTAAAAAGTTGGAGGAACTTGGAAATGTGTTTACAAGTTTGGATCCTGGTGACTCCATTGTTGTGGCCAAGTCCTTTTCCCACATGCTTAACTTGGCCAATTTGGCTGAAGAAGTCCAGATCGCCTATCGGAGAAGGATAAAGTTGAAGAAGGGAGATTTTGTTGATGAAGCAAATGCTACAACGGAGTCAGACATTGAAGAGACATTCAAGAAGCTTGTTGGGCAACTGAAGAAGTCCCCACAAGAAGTTTTTGATGAATTGAAGAACCAGACTATAGATTTGGTTCTAACCGCACATCCTACTCAGTCTGTTCGCAGATCTCTGCTGCAAAAGCATGCAAGGGTAAGAAATTGTCTGACACAGTTGTATGCCAAGGATATTACACCTGATGATAAACAAGAACTCGATGAGGCTCTACAAAGGGAGATTCAAGCTGCATTTCGCACTGATGAAATCCGAAGGACTCCTCCAACCCCTCAAGATGAGATGAGGGCAGGAATGAGCTACTTCCATGAGACAATTTGGAAAGGTGTGCCAAAATTCTTACGACGAGTTGACACTGCGTTGAAGAACATTGGGATAAATGAACGTGTTCCGTACAATGCCCCTCTCATTCAATTTTCTTCTTGGATGGGTGGGGATCGTGATGGAAATCCCAGGGTCACTCCAGAAGTTACAAGGGATGTATGCTTATTGGCTAGAATGATGGCTGCTAACTTGTATTTCTCCCAGATAGAGGATCTTATGTTTGAGTTATCTATGTGGCGTTGCAACGAAGAGCTTCTTGCTCGTGCTCATGAACTTCATAGGTCTTCAAGGAAGGATGCAAAACACTACATAGAGTTTTGGAAACAAATTCCTCCGAACGAGCCATATCGTGTTATTCTTGGTGGTGTGAGAGACAAGCTCTACAATACACGTGAACGTGCTCGTCAACTATTATCCAGTCTAACCTCTGACATTCCTGAGGATACAACTTTTACTAGTGTTGAACAGTTCCTGGAGCCTCTTGAACTGTGTTACCGGTCACTCTGTTCCTGTGGTGACAAGGCAATTGCTGATGGGAGCCTTCTTGATTTCTTACGGCAAGTTTCTACTTTCGGCCTTTCTCTTGTGAGACTAGACATACGGCAAGAATCAGACAGGCACACTGATGTCATTGATACTATCACAAAGCACCTGGGCCTTGGGTCTTATCGAGAATGGCCTGAGAAACAAAGGCAAGAATGGTTGTTGTCTGAGCTCAGTGGAAAGCGCCCTCTTTTTGGCGCTGATATTCCCAAAACCGAAGAAGTTTCTGATGTATTGGAGACATTCAAAGTAATATCAGAACTTCCCTCAGACAACTTTGGTGCCTATATCATATCAATGGCAACAGCCCCATCCGATGTACTTGCTGTTGAGCTTTTACAACGTGAATGCCATGTGAAAAAGCCACTAAGGGTTGTACCGTTGTTTGAGAAGCTTGCTGATTTAGAGGCTGCTCCTGCTGCTGTTGCTCGCCTCTTTTCTATAGATTGGTACAAAAACCGGATCAATGGGAAGCAAGAAGTCATGATAGGGTATTCAGATTCAGGAAAGGATGCTGGCCGTCTATCTGCAGCATGGCAGCTATACAAGGCTCAAGAAGAGCTCGTAAAGGTTGCAAAACAATATGGAGTTAAGCTAACCATGTTTCATGGCCGAGGTGGAACAGTTGGAAGAGGAGGGGGACCCACTCATCTTGCTATATTGTCTCAGCCACCTGAGACAATTCATGGTTCGCTCCGTGTGACCGTTCAAGGAGAAGTTATCGAACAATCATTTGGAGAGGAGCACTTGTGTTTTAGAACACTCCAGCGTTTCACAGCAGCTACACTAGAGCATGGCATGAATCCCCCAGTTTCACCAAAGCCAGAATGGCGTGCACTCATGGATGAGATGGCAGTTGTTGCTACAGAGGAATACCGTTCAATTGTTTTTCAGGAACCTCGTTTTGTTGAATACTTTCGCCTCGCAACACCAGAGATGGAGTATGGTCGGATGAACATTGGGAGTCGTCCATCAAAGCGAAAGCCAAGTGGAGGCATCGAATCACTTCGAGCAATCCCCTGGATCTTTGCATGGACCCAGACAAGATTTCATTTACCAGTGTGGCTTGGTTTTGGGGCAGCATTTAAGCAAGCCATAGAGAAGGATGCAAAGAATCTTCAGATGCTTCGGGAGATGTATAATCAGTGGCCGTTCTTCAGAGTCACCATTGATTTGATTGAGATGGTGTTTGCCAAGGGAGACCCAGGTATTGCAGCACTTTATGACAAGCTTCTGGTGTCAGAAGACCTATGGTCGTTTGGGGAGCGACTGAGGACCAACTATGAAGATACTAAACGCCTCCTACTCCAGGTTGCTGGGCACAAAGCTCTTCTGGAGGGAGATCCTTACTTGAGGCAGAGACTTGTTCTTCGTGACTCGTATATTACAGCACTTAATGTGTGCCAAGCCTATACACTGAAGCAGATCCGTGACCCCAACTACCTTGTTCAAGTAAGGCCGTCAAAGGAACCAGCAAGCAAGCCAGCAGCAGAGCTCGTCAATCTTAACCCAACAAGCGAATATGCTCCAGGTCTAGAGGACACACTTATTTTGACCATGAAGGGTATAGCTGCTGGAATGCAGAACACTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0008152 GO:0008964 GO:0009506 GO:0009507 GO:0009536 GO:0015977 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0030054 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055044 GO:0071704 GO:0071944 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

962

Amino Acids

109.41

Weight (kDa)

5.86

Isoelectric Point (pI)

45.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	34 - 961	0.0	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005600)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42600 AT2G42600 AT2G42600
fragaria_vesca	FvH4_6g27390 FvH4_6g27390 FvH4_6g27390 FvH4_6g33320
malus_domestica	MD09G1237900.v1.1 MD17G1230800.v1.1
prunus_persica	Prupe.3G118300_v2.0.a1
pyrus_communis	pycom09g15640 pycom17g23400
rosa_chinensis	RchiOBHm_Chr2g0090101 RchiOBHm_Chr2g0129661
rosa_laevigata	RLG00000016085 RLG00000019094
rosa_multiflora	Rmu_sc0008968.1_g000006 Rmu_sc0009263.1_g000005 Rmu_sc0020548.1_g000003
rosa_roxburghii	Rroxscaffold_2G00114470
rosa_rugosa	Rorug02G0005300 Rorug02G0288200
rosa_samantha	Rh2AG339500 Rh2BG348300 Rh2CG326900 Rh2DG366400
rosa_wichuraiana	Rw2G004560 Rw2G027850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	42
AccB1I	GGYRCC	2 cut(s)	58, 1571
AccII	CGCG	1 cut(s)	194
AciI	CCGC	3 cut(s)	192, 194, 510
AcoI	YGGCCR	5 cut(s)	282, 312, 1807, 1900, 2438
AcsI	RAATTY	3 cut(s)	13, 758, 1074
AcuI	CTGAAG	6 cut(s)	119, 345, 467, 2393, 2431, 2729
AcvI	CACGTG	1 cut(s)	1139
AfaI	GTAC	4 cut(s)	818, 1611, 1668, 1742
AfiI	CCNNNNNNNGG	4 cut(s)	955, 1484, 2282, 2599
AflIII	ACRYGT	2 cut(s)	808, 1136
AgeI	ACCGGT	1 cut(s)	1242
AhdI	GACNNNNNGTC	1 cut(s)	2542
AhlI	ACTAGT	1 cut(s)	1202
AjnI	CCWGG	8 cut(s)	262, 875, 1218, 1398, 2309, 2494, 2598, 2818
AjuI	GAANNNNNNNTTGG	2 cut(s)	2007, 2039
Alw21I	GWGCWC	7 cut(s)	1015, 1150, 1464, 1854, 2037, 2128, 2787
Alw26I	GTCTC	9 cut(s)	413, 728, 1113, 1330, 1523, 1959, 1961, 2484, 2644
Alw44I	GTGCAC	1 cut(s)	2124
AlwNI	CAGNNNCTG	1 cut(s)	1219
AoxI	GGCC	9 cut(s)	282, 312, 1321, 1402, 1424, 1807, 1900, 2438, 2747
ApaLI	GTGCAC	1 cut(s)	2124
ApoI	RAATTY	3 cut(s)	13, 758, 1074
ArsI	GACNNNNNNTTYG	4 cut(s)	481, 513, 2643, 2675
AsiGI	ACCGGT	1 cut(s)	1242
Asp700I	GAANNNNTTC	5 cut(s)	168, 810, 999, 2205, 2295
AspLEI	GCGC	3 cut(s)	43, 1476, 1490
AspS9I	GGNCC	5 cut(s)	214, 1402, 1931, 2325, 2565
AsuC2I	CCSGG	1 cut(s)	63
AsuHPI	GGTGA	4 cut(s)	278, 1274, 2097, 2446
AvaII	GGWCC	4 cut(s)	214, 1931, 2325, 2565
AxyI	CCTNAGG	1 cut(s)	1185
BaeGI	GKGCMC	2 cut(s)	2128, 2613
BalI	TGGCCA	2 cut(s)	284, 314
BamHI	GGATCC	1 cut(s)	259
BanI	GGYRCC	2 cut(s)	58, 1571
BanII	GRGCYC	3 cut(s)	1464, 1854, 2787
BauI	CACGAG	1 cut(s)	1008
BbrPI	CACGTG	1 cut(s)	1139
BbsI	GAAGAC	2 cut(s)	1023, 2541
Bbv12I	GWGCWC	7 cut(s)	1015, 1150, 1464, 1854, 2037, 2128, 2787
BbvCI	CCTCAGC	1 cut(s)	47
BceAI	ACGGC	5 cut(s)	1319, 1364, 1794, 2425, 2734
BcgI	CGANNNNNNTGC	4 cut(s)	83, 117, 1595, 1629
BciT130I	CCWGG	8 cut(s)	264, 877, 1220, 1400, 2311, 2496, 2600, 2820
BciVI	GTATCC	1 cut(s)	1183
BcnI	CCSGG	1 cut(s)	63
BcoDI	GTCTC	9 cut(s)	413, 728, 1113, 1330, 1523, 1959, 1961, 2484, 2644
BcuI	ACTAGT	1 cut(s)	1202
BfaI	CTAG	5 cut(s)	918, 1203, 1340, 2078, 2825
BfmI	CTRYAG	4 cut(s)	492, 1731, 1818, 2154
BfoI	RGCGCY	2 cut(s)	1477, 1491
BfuI	GTATCC	1 cut(s)	1183
BglI	GCCNNNNNGGC	2 cut(s)	320, 2119
BglII	AGATCT	1 cut(s)	536
Bme1390I	CCNGG	9 cut(s)	63, 264, 877, 1220, 1400, 2311, 2496, 2600, 2820
Bme18I	GGWCC	4 cut(s)	214, 1931, 2325, 2565
BmeRI	GACNNNNNGTC	1 cut(s)	2542
BmgT120I	GGNCC	5 cut(s)	214, 1402, 1931, 2325, 2565
BmrFI	CCNGG	9 cut(s)	63, 264, 877, 1220, 1400, 2311, 2496, 2600, 2820
BmrI	ACTGGG	1 cut(s)	2093
BmsI	GCATC	7 cut(s)	28, 91, 1033, 1792, 2295, 2386, 2403
BmuI	ACTGGG	1 cut(s)	2093
BpiI	GAAGAC	2 cut(s)	1023, 2541
BplI	GAGNNNNNCTC	2 cut(s)	1110, 1142
BpmI	CTGGAG	6 cut(s)	870, 1241, 2039, 2582, 2645, 2802
Bpu10I	CCTNAGC	1 cut(s)	47
BpuEI	CTTGAG	3 cut(s)	681, 1827, 2662
BpuMI	CCSGG	1 cut(s)	63
BsaAI	YACGTR	1 cut(s)	1139
BsaBI	GATNNNNATC	1 cut(s)	870
BsaI	GGTCTC	1 cut(s)	2484
BsaWI	WCCGGW	2 cut(s)	1242, 1749
BsaXI	ACNNNNNCTCC	4 cut(s)	363, 393, 2024, 2054
Bsc4I	CCNNNNNNNGG	4 cut(s)	955, 1484, 2282, 2599
Bse118I	RCCGGY	1 cut(s)	1242
Bse1I	ACTGG	4 cut(s)	1164, 2099, 2348, 2884
Bse21I	CCTNAGG	1 cut(s)	1185
Bse8I	GATNNNNATC	1 cut(s)	870
BseBI	CCWGG	8 cut(s)	264, 877, 1220, 1400, 2311, 2496, 2600, 2820
BseJI	GATNNNNATC	1 cut(s)	870
BseLI	CCNNNNNNNGG	4 cut(s)	955, 1484, 2282, 2599
BseNI	ACTGG	4 cut(s)	1164, 2099, 2348, 2884
BseRI	GAGGAG	3 cut(s)	675, 1938, 2045
BseSI	GKGCMC	2 cut(s)	2128, 2613
BseYI	CCCAGC	1 cut(s)	2606
Bsh1236I	CGCG	1 cut(s)	194
BshFI	GGCC	9 cut(s)	284, 314, 1323, 1404, 1426, 1809, 1902, 2440, 2749
BshNI	GGYRCC	2 cut(s)	58, 1571
BshTI	ACCGGT	1 cut(s)	1242
BsiHKAI	GWGCWC	7 cut(s)	1015, 1150, 1464, 1854, 2037, 2128, 2787
BsiSI	CCGG	3 cut(s)	62, 1243, 1750
BslFI	GGGAC	2 cut(s)	439, 1944
BslI	CCNNNNNNNGG	4 cut(s)	955, 1484, 2282, 2599
BsmAI	GTCTC	9 cut(s)	413, 728, 1113, 1330, 1523, 1959, 1961, 2484, 2644
BsmFI	GGGAC	2 cut(s)	439, 1944
BsmI	GAATGC	2 cut(s)	1643, 2874
BsnI	GGCC	9 cut(s)	284, 314, 1323, 1404, 1426, 1809, 1902, 2440, 2749
Bso31I	GGTCTC	1 cut(s)	2484
Bsp1286I	GDGCHC	8 cut(s)	1015, 1150, 1464, 1854, 2037, 2128, 2613, 2787
BspACI	CCGC	3 cut(s)	192, 194, 510
BspANI	GGCC	9 cut(s)	284, 314, 1323, 1404, 1426, 1809, 1902, 2440, 2749
BspFNI	CGCG	1 cut(s)	194
BspHI	TCATGA	2 cut(s)	1015, 1773
BspMAI	CTGCAG	1 cut(s)	1822
BspQI	GCTCTTC	3 cut(s)	990, 1842, 2625
BspT107I	GGYRCC	2 cut(s)	58, 1571
BspTNI	GGTCTC	1 cut(s)	2484
BsrFI	RCCGGY	1 cut(s)	1242
BsrI	ACTGG	4 cut(s)	1164, 2099, 2348, 2884
BssAI	RCCGGY	1 cut(s)	1242
BssSI	CACGAG	1 cut(s)	1008
BssT1I	CCWWGG	3 cut(s)	591, 1405, 2484
Bst2BI	CACGAG	1 cut(s)	1008
Bst2UI	CCWGG	8 cut(s)	264, 877, 1220, 1400, 2311, 2496, 2600, 2820
Bst4CI	ACNGT	8 cut(s)	169, 582, 1215, 1236, 1671, 1915, 1996, 2168
Bst6I	CTCTTC	5 cut(s)	411, 990, 1842, 1915, 2625
BstAPI	GCANNNNNTGC	1 cut(s)	2150
BstBAI	YACGTR	1 cut(s)	1139
BstC8I	GCNNGC	9 cut(s)	555, 1686, 1720, 1807, 2124, 2382, 2767, 2771, 2775
BstDSI	CCRYGG	1 cut(s)	192
BstENI	CCTNNNNNAGG	1 cut(s)	2597
BstFNI	CGCG	1 cut(s)	194
BstH2I	RGCGCY	2 cut(s)	1477, 1491
BstHHI	GCGC	3 cut(s)	43, 1476, 1490
BstMAI	GTCTC	9 cut(s)	413, 728, 1113, 1330, 1523, 1959, 1961, 2484, 2644
BstMWI	GCNNNNNNNGC	9 cut(s)	49, 320, 439, 550, 1706, 1709, 1715, 2119, 2150
BstNI	CCWGG	8 cut(s)	264, 877, 1220, 1400, 2311, 2496, 2600, 2820
BstNSI	RCATGY	2 cut(s)	304, 557
BstSCI	CCNGG	9 cut(s)	61, 262, 875, 1218, 1398, 2309, 2494, 2598, 2818
BstSFI	CTRYAG	4 cut(s)	492, 1731, 1818, 2154
BstSLI	GKGCMC	2 cut(s)	2128, 2613
BstUI	CGCG	1 cut(s)	194
BstV2I	GAAGAC	2 cut(s)	1023, 2541
BstX2I	RGATCY	7 cut(s)	154, 259, 536, 958, 2313, 2632, 2715
BstXI	CCANNNNNNTGG	2 cut(s)	280, 2606
BstYI	RGATCY	7 cut(s)	154, 259, 536, 958, 2313, 2632, 2715
Bsu36I	CCTNAGG	1 cut(s)	1185
BsuI	GTATCC	1 cut(s)	1183
BsuRI	GGCC	9 cut(s)	284, 314, 1323, 1404, 1426, 1809, 1902, 2440, 2749
BtgI	CCRYGG	1 cut(s)	192
BtsI	GCAGTG	1 cut(s)	777
BtsIMutI	CAGTG	8 cut(s)	663, 777, 1368, 1471, 2355, 2441, 2705, 2877
Cac8I	GCNNGC	9 cut(s)	555, 1686, 1720, 1807, 2124, 2382, 2767, 2771, 2775
CaiI	CAGNNNCTG	1 cut(s)	1219
CciI	TCATGA	2 cut(s)	1015, 1773
CfoI	GCGC	3 cut(s)	43, 1476, 1490
Cfr10I	RCCGGY	1 cut(s)	1242
Cfr13I	GGNCC	5 cut(s)	214, 1402, 1931, 2325, 2565
Cfr42I	CCGCGG	1 cut(s)	195
Csp6I	GTAC	4 cut(s)	817, 1610, 1667, 1741
CspAI	ACCGGT	1 cut(s)	1242
CspCI	CAANNNNNGTGG	2 cut(s)	1924, 1959
CviQI	GTAC	4 cut(s)	817, 1610, 1667, 1741
DriI	GACNNNNNGTC	1 cut(s)	2542
EaeI	YGGCCR	5 cut(s)	282, 312, 1807, 1900, 2438
Eam1104I	CTCTTC	5 cut(s)	411, 990, 1842, 1915, 2625
Eam1105I	GACNNNNNGTC	1 cut(s)	2542
EarI	CTCTTC	5 cut(s)	411, 990, 1842, 1915, 2625
Ecl136II	GAGCTC	3 cut(s)	1462, 1852, 2785
Eco130I	CCWWGG	3 cut(s)	591, 1405, 2484
Eco24I	GRGCYC	3 cut(s)	1464, 1854, 2787
Eco31I	GGTCTC	1 cut(s)	2484
Eco47I	GGWCC	4 cut(s)	214, 1931, 2325, 2565
Eco53kI	GAGCTC	3 cut(s)	1462, 1852, 2785
Eco57I	CTGAAG	6 cut(s)	119, 345, 467, 2393, 2431, 2729
Eco72I	CACGTG	1 cut(s)	1139
Eco81I	CCTNAGG	1 cut(s)	1185
EcoICRI	GAGCTC	3 cut(s)	1462, 1852, 2785
EcoNI	CCTNNNNNAGG	1 cut(s)	2597
EcoO109I	RGGNCCY	2 cut(s)	214, 1931
EcoRII	CCWGG	8 cut(s)	262, 875, 1218, 1398, 2309, 2494, 2598, 2818
EcoT14I	CCWWGG	3 cut(s)	591, 1405, 2484
EcoT38I	GRGCYC	3 cut(s)	1464, 1854, 2787
ErhI	CCWWGG	3 cut(s)	591, 1405, 2484
FalI	AAGNNNNNCTT	2 cut(s)	987, 1019
FaqI	GGGAC	2 cut(s)	439, 1944
FriOI	GRGCYC	3 cut(s)	1464, 1854, 2787
FspBI	CTAG	5 cut(s)	918, 1203, 1340, 2078, 2825
FspI	TGCGCA	1 cut(s)	42
GlaI	GCGC	3 cut(s)	42, 1475, 1489
GsaI	CCCAGC	1 cut(s)	2610
GsuI	CTGGAG	6 cut(s)	870, 1241, 2039, 2582, 2645, 2802
HaeII	RGCGCY	2 cut(s)	1477, 1491
HaeIII	GGCC	9 cut(s)	284, 314, 1323, 1404, 1426, 1809, 1902, 2440, 2749
HapII	CCGG	3 cut(s)	62, 1243, 1750
HhaI	GCGC	3 cut(s)	43, 1476, 1490
Hin6I	GCGC	3 cut(s)	41, 1474, 1488
HinP1I	GCGC	3 cut(s)	41, 1474, 1488
HincII	GTYRAC	2 cut(s)	775, 1154
HindII	GTYRAC	2 cut(s)	775, 1154
HindIII	AAGCTT	3 cut(s)	431, 1682, 2519
HpaII	CCGG	3 cut(s)	62, 1243, 1750
HphI	GGTGA	4 cut(s)	278, 1274, 2097, 2446
Hpy166II	GTNNAC	5 cut(s)	249, 775, 1142, 1154, 2126
Hpy8I	GTNNAC	5 cut(s)	249, 775, 1142, 1154, 2126
Hpy99I	CGWCG	1 cut(s)	771
HpyAV	CCTTC	8 cut(s)	197, 345, 361, 672, 1033, 1298, 2386, 2845
HpyCH4III	ACNGT	8 cut(s)	169, 582, 1215, 1236, 1671, 1915, 1996, 2168
HpyCH4IV	ACGT	4 cut(s)	808, 1138, 1144, 1633
HpyF10VI	GCNNNNNNNGC	9 cut(s)	49, 320, 439, 550, 1706, 1709, 1715, 2119, 2150
HpySE526I	ACGT	4 cut(s)	808, 1138, 1144, 1633
HspAI	GCGC	3 cut(s)	41, 1474, 1488
KflI	GGGWCCC	1 cut(s)	1931
KspI	CCGCGG	1 cut(s)	195
LguI	GCTCTTC	3 cut(s)	990, 1842, 2625
LmnI	GCTCC	7 cut(s)	1222, 1284, 1708, 1989, 2032, 2554, 2821
LweI	GCATC	7 cut(s)	28, 91, 1033, 1792, 2295, 2386, 2403
MaeI	CTAG	5 cut(s)	918, 1203, 1340, 2078, 2825
MaeII	ACGT	4 cut(s)	808, 1138, 1144, 1633
MfeI	CAATTG	3 cut(s)	33, 1272, 2172
MflI	RGATCY	7 cut(s)	154, 259, 536, 958, 2313, 2632, 2715
MhlI	GDGCHC	8 cut(s)	1015, 1150, 1464, 1854, 2037, 2128, 2613, 2787
MlsI	TGGCCA	2 cut(s)	284, 314
MluNI	TGGCCA	2 cut(s)	284, 314
MlyI	GAGTC	6 cut(s)	263, 413, 676, 2263, 2460, 2660
MmeI	TCCRAC	5 cut(s)	93, 207, 713, 1897, 2219
Mox20I	TGGCCA	2 cut(s)	284, 314
MroXI	GAANNNNTTC	5 cut(s)	168, 810, 999, 2205, 2295
MscI	TGGCCA	2 cut(s)	284, 314
MseI	TTAA	5 cut(s)	306, 1881, 2376, 2685, 2796
MslI	CAYNNNNRTG	3 cut(s)	278, 1371, 1586
Msp20I	TGGCCA	2 cut(s)	284, 314
MspA1I	CMGCKG	2 cut(s)	46, 194
MspI	CCGG	3 cut(s)	62, 1243, 1750
MspR9I	CCNGG	9 cut(s)	63, 264, 877, 1220, 1400, 2311, 2496, 2600, 2820
MunI	CAATTG	3 cut(s)	33, 1272, 2172
Mva1269I	GAATGC	2 cut(s)	1643, 2874
MvaI	CCWGG	8 cut(s)	264, 877, 1220, 1400, 2311, 2496, 2600, 2820
MvnI	CGCG	1 cut(s)	194
MwoI	GCNNNNNNNGC	9 cut(s)	49, 320, 439, 550, 1706, 1709, 1715, 2119, 2150
NciI	CCSGG	1 cut(s)	63
NmeAIII	GCCGAG	1 cut(s)	1928
NmuCI	GTSAC	8 cut(s)	266, 880, 1245, 1262, 1990, 2452, 2663, 2720
NsbI	TGCGCA	1 cut(s)	42
NspI	RCATGY	2 cut(s)	304, 557
PaeI	GCATGC	1 cut(s)	557
PagI	TCATGA	2 cut(s)	1015, 1773
PasI	CCCWGGG	1 cut(s)	876
PciSI	GCTCTTC	3 cut(s)	990, 1842, 2625
PctI	GAATGC	2 cut(s)	1643, 2874
PdmI	GAANNNNTTC	5 cut(s)	168, 810, 999, 2205, 2295
PfeI	GAWTC	6 cut(s)	646, 1355, 1790, 2092, 2291, 2404
PfoI	TCCNGGA	1 cut(s)	1218
PinAI	ACCGGT	1 cut(s)	1242
PleI	GAGTC	6 cut(s)	263, 412, 676, 2262, 2459, 2660
PmaCI	CACGTG	1 cut(s)	1139
PmlI	CACGTG	1 cut(s)	1139
PpsI	GAGTC	6 cut(s)	263, 412, 676, 2262, 2459, 2660
Ppu21I	YACGTR	1 cut(s)	1139
PpuMI	RGGWCCY	2 cut(s)	214, 1931
Psp124BI	GAGCTC	3 cut(s)	1464, 1854, 2787
Psp5II	RGGWCCY	2 cut(s)	214, 1931
Psp6I	CCWGG	8 cut(s)	262, 875, 1218, 1398, 2309, 2494, 2598, 2818
PspCI	CACGTG	1 cut(s)	1139
PspFI	CCCAGC	1 cut(s)	2606
PspGI	CCWGG	8 cut(s)	262, 875, 1218, 1398, 2309, 2494, 2598, 2818
PspPI	GGNCC	5 cut(s)	214, 1402, 1931, 2325, 2565
PspPPI	RGGWCCY	2 cut(s)	214, 1931
PstI	CTGCAG	1 cut(s)	1822
PstNI	CAGNNNCTG	1 cut(s)	1219
PsuI	RGATCY	7 cut(s)	154, 259, 536, 958, 2313, 2632, 2715
PvuII	CAGCTG	1 cut(s)	46
RsaI	GTAC	4 cut(s)	818, 1611, 1668, 1742
RsaNI	GTAC	4 cut(s)	817, 1610, 1667, 1741
RseI	CAYNNNNRTG	3 cut(s)	278, 1371, 1586
SacI	GAGCTC	3 cut(s)	1464, 1854, 2787
SacII	CCGCGG	1 cut(s)	195
SapI	GCTCTTC	3 cut(s)	990, 1842, 2625
SaqAI	TTAA	5 cut(s)	306, 1881, 2376, 2685, 2796
Sau96I	GGNCC	5 cut(s)	214, 1402, 1931, 2325, 2565
SchI	GAGTC	6 cut(s)	263, 413, 676, 2263, 2460, 2660
ScrFI	CCNGG	9 cut(s)	63, 264, 877, 1220, 1400, 2311, 2496, 2600, 2820
SduI	GDGCHC	8 cut(s)	1015, 1150, 1464, 1854, 2037, 2128, 2613, 2787
SfaNI	GCATC	7 cut(s)	28, 91, 1033, 1792, 2295, 2386, 2403
SfcI	CTRYAG	4 cut(s)	492, 1731, 1818, 2154
Sfr303I	CCGCGG	1 cut(s)	195
SgrBI	CCGCGG	1 cut(s)	195
SinI	GGWCC	4 cut(s)	214, 1931, 2325, 2565
SmiMI	CAYNNNNRTG	3 cut(s)	278, 1371, 1586
SmlI	CTYRAG	3 cut(s)	696, 1842, 2641
SmoI	CTYRAG	3 cut(s)	696, 1842, 2641
SpeI	ACTAGT	1 cut(s)	1202
SphI	GCATGC	1 cut(s)	557
SsiI	CCGC	3 cut(s)	192, 194, 510
SspMI	CTAG	5 cut(s)	918, 1203, 1340, 2078, 2825
SstI	GAGCTC	3 cut(s)	1464, 1854, 2787
StyD4I	CCNGG	9 cut(s)	61, 262, 875, 1218, 1398, 2309, 2494, 2598, 2818
StyI	CCWWGG	3 cut(s)	591, 1405, 2484
TaaI	ACNGT	8 cut(s)	169, 582, 1215, 1236, 1671, 1915, 1996, 2168
TaiI	ACGT	4 cut(s)	811, 1141, 1147, 1636
TaqI	TCGA	7 cut(s)	8, 93, 624, 1417, 2013, 2289, 2299
TatI	WGTACW	1 cut(s)	1609
TfiI	GAWTC	6 cut(s)	646, 1355, 1790, 2092, 2291, 2404
Tru1I	TTAA	5 cut(s)	306, 1881, 2376, 2685, 2796
Tru9I	TTAA	5 cut(s)	306, 1881, 2376, 2685, 2796
TscAI	CASTG	8 cut(s)	670, 784, 1375, 1471, 2355, 2441, 2712, 2884
TseFI	GTSAC	8 cut(s)	266, 880, 1245, 1262, 1990, 2452, 2663, 2720
Tsp45I	GTSAC	8 cut(s)	266, 880, 1245, 1262, 1990, 2452, 2663, 2720
TspGWI	ACGGA	4 cut(s)	416, 804, 1976, 2708
TspRI	CASTG	8 cut(s)	670, 784, 1375, 1471, 2355, 2441, 2712, 2884
VneI	GTGCAC	1 cut(s)	2124
VpaK11BI	GGWCC	4 cut(s)	214, 1931, 2325, 2565
XagI	CCTNNNNNAGG	1 cut(s)	2597
XapI	RAATTY	3 cut(s)	13, 758, 1074
XbaI	TCTAGA	1 cut(s)	2824
XceI	RCATGY	2 cut(s)	304, 557
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2115
XmnI	GAANNNNTTC	5 cut(s)	168, 810, 999, 2205, 2295
XspI	CTAG	5 cut(s)	918, 1203, 1340, 2078, 2825

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.