Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0005600

Physical Location & Seq

Genomic Coordinates Reverse (-)

3785991 .. 3788876

Gene Structure 2886 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016085

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2886 bp

ATGGGAGCGAGGAACTTGGAGAAGATGGCTTCAATTGATGCACATCTGAGGCTTTTGGCACCAGGGAAGGTGTCTGAGGATGACAAACTGGTCGAGTATGATGCTTTGTTGTTGGATCGTTTCCTCGATATTCTTCAGGATTTACATGGGGAGGATCTCAGACAAACTGTTCAAGACTGTTATGAGCTATCCGCGGAATACGAAGGAAAGAAGGACCCTAAAAAGTTAGAGGAACTCGGAAATGTGTTAACGAGTTTGGATCCTGGTGACTCCATTGTTGTTGCGAAGTCTTTTTCCCACATGCTTAACTTGGCCAATTTGGCTGAAGAAGTTCAGATTGCATATCGGAGAAGGATAAAGTTGAAGAAGGGAGATTTTGTTGATGAAGCAAATGCCACTACGGAGTCGGACATTGAAGAGACGTTCAAGAAGCTTGTGGGGCAACTGAACAAGTCCCCACAACAAATTTTCGATGAGTTGAAGAACCAGACTGTAGATTTGGTTCTAACCGCACATCCTACTCAGTCTGTTCGCAGATCTCTGCTTCAAAAGCATGCTAGGGTAAGAGATTGTATGACGCAGTTGTATGCCAAGGATATTACACCTGATGATAAACAAGAACTCGATGAGGCTCTACAAAGGGAGATTCAAGCTGCATTTCGCACCGACGAAATCCGAAGGACTCCTCCAACCCCTCAAGATGAGATGAGGGCAGGAATGAGCTACTTTCATGAGACGATTTGGAAAGGTGTACCGAAATTCTTACGCCGAGTTGACACTGCCTTGAAGAGCATTGGGATAAACGAACGTGTTCCTTACAATGCCCCTCTCATTCAATTTTCTTCTTGGATGGGTGGGGATCGTGATGGAAATCCCAGGGTCACTCCAGAAGTTACACGTGATGTATGCTTATTGGCTAGAATGATGGCTGCTAACTTGTACTTCTCCCAGATAGAGGATCTCATGTTTGAGTTATCTATGTGGCGTTGCAACGATGAGCTTCTTGCTCGCGCTCATGAACTTTATAGGTCTTCAAGGAAGTATGCAAAACACTACATAGAGTTTTGGCAACAAATTCCTCCAAATGAACCATATCGTGTTATTCTTGGTGATGTGAGAGACAAGCTCTACAATACACGTGAACGTGCTCGTCAATTATTATCCAATGGAACCTCTGACATTCCCGAGGACACAACTTTTACTAATGTTGAACACTTCCTGGCGCCTCTCGAACTGTGTTACCGGTCACTGTGTTCCTGTGGTGACCAGCCAATTGCCGACGGGAGCCTTCTTGATTTCTTACGTCAAGTTTCTACTTTCGGACTTTCTCTTGTGAGACTAGACATACGGCAAGAATCAGAGAGGCACACTGATGTCATTGATGCTATTACAAAGCACCTGGGCCTCGGGTCTTATCGAGAATGGCCCGAGAAACAAAGGCAACAATGGCTCTTGTCTGAGCTCAAGGGAAAGCGCCCTCTTTTTGGCACTGATGTTCCCAAGACTGAAGAAATTTCTGAAGTATTGGAGACATTCAAAGTCATTTCAGAACTTCCCTCAGACAACTTTGGGGCCTATATTATATCAATGGCAACAGCCCCATCTGATGTACTTGCTGTTGAGCTTTTACAACGTGAATGCCGTGTGAAAACGCCACTAAGGGTTGTTCCATTGTTTGAGAAGCTAGCTGATTTAGAGGCTGCTCCTGCTGCTGTGGCTCGCCTCTTCTCTATGGATTGGTACAAAAACCGGATCAATGGGAAGCAAGAAGTCATGATAGGTTACTCGGATTCAGGAAAGGATGCTGGCCGTCTATCTGCAGCATGGCATCTATACAAGGCTCAAGAAGAGCTCGTAAAGGTTGCGAAACAATATGGAGTTAAGCTCACCATGTTTCATGGCCGAGGTGGAACAGTGGGAAGAGGAGGGGGACCAACTCATCTTGCTATATTGTCTCAGCCACCTGAGACGATTCATGGTTCGCTCCGTGTAACAGTTCAAGGTGAAGTTATTGAACGATCATTTGGAGAGGAGCACTTGTGTTTTAGAACACTAGAGCGTTTCACAGCAGCTACACTAGAGCATGGCATGAATCCCCCAGTTTCTCCCAAGCCAGAATGGCGTGCACTCATGGATGAGATGGCAGTTGTTGCTACGGAGGAATACCGTTCAATAGTTTTCCAGGAACCCCGTTTTGTTGAGTACTTTCGCCTTGCAACACCGGAGATGGAGTATGGTAGGATGAACATTGGGAGTCGTCCATCAAAGCGAAACCAAAGTGGAGGCATCGAATCACTTCGAGCAATCCCATGGATCTTTGCATGGACCCAGACAAGGTTTCACTTACCAGTGTGGCTTGGTTTTGGGGCAGCATTTAAGCAAGCCATAGAGAATGATGCAAAGAATCTTCAGATGCTTCGTGACATGTATAATCAGTGGCCTTTCTTCAGAGTCACCATTGATTTAATTGAGATGGTGTTTGCCAAGGGAGACCCAGGTATTACAGCTCTTTATGACAAGCTTCTGGTGTCACAAGACCTATGGCTATTTGGAAAGCGACTGAGGACCAACTATGAAGATACTAAACGCCTCCTACTCCAGGTTGCTGGGCACAGAGATCTTCTGGAAGGAGATCCTTACTTAAGGCAGAGACTTCTTCTTCGTGACTCGTATATTACAACACTAAATGTATGCCAAGCCTATACGCTGAAGCAGATCCGTGACCCCAACTATCTTGTTCAAGTAAGGTCATCAAAGGAACCGGCAAGCAAGCCAGCAGCAGAGCTCGTCAAGCTTAACCCGACAAGCGAATATGCTCCAGGTCTAGAGGACACACTTATTTTGACCATGAAGGGTATTGCTGCTGGGATGCAGAACACCGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004611 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0008152 GO:0008964 GO:0009506 GO:0009507 GO:0009536 GO:0015977 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0030054 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055044 GO:0071704 GO:0071944 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEPcase

Protein Analysis

962

Amino Acids

109.69

Weight (kDa)

6.05

Isoelectric Point (pI)

43.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEPcase	PF00311	34 - 961	0.0	Phosphoenolpyruvate carboxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005600)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42600 AT2G42600 AT2G42600
fragaria_vesca	FvH4_6g27390 FvH4_6g27390 FvH4_6g27390 FvH4_6g33320
malus_domestica	MD09G1237900.v1.1 MD17G1230800.v1.1
prunus_persica	Prupe.3G118300_v2.0.a1
pyrus_communis	pycom09g15640 pycom17g23400
rosa_chinensis	RchiOBHm_Chr2g0090101 RchiOBHm_Chr2g0129661
rosa_laevigata	RLG00000016085 RLG00000019094
rosa_multiflora	Rmu_sc0008968.1_g000006 Rmu_sc0009263.1_g000005 Rmu_sc0020548.1_g000003
rosa_roxburghii	Rroxscaffold_2G00114470
rosa_rugosa	Rorug02G0005300 Rorug02G0288200
rosa_samantha	Rh2AG339500 Rh2BG348300 Rh2CG326900 Rh2DG366400
rosa_wichuraiana	Rw2G004560 Rw2G027850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	89
AccB1I	GGYRCC	2 cut(s)	58, 1222
AccII	CGCG	2 cut(s)	194, 1011
AciI	CCGC	3 cut(s)	192, 194, 510
AcoI	YGGCCR	3 cut(s)	312, 1807, 1900
AcsI	RAATTY	4 cut(s)	465, 758, 1074, 1512
AcuI	CTGAAG	7 cut(s)	119, 345, 1527, 1539, 2393, 2431, 2729
AcvI	CACGTG	2 cut(s)	899, 1139
AcyI	GRCGYC	1 cut(s)	1223
AfaI	GTAC	5 cut(s)	753, 941, 1611, 1742, 2204
AfiI	CCNNNNNNNGG	4 cut(s)	955, 1484, 2334, 2599
AflII	CTTAAG	1 cut(s)	2641
AflIII	ACRYGT	4 cut(s)	808, 896, 1136, 2424
AgeI	ACCGGT	2 cut(s)	1242, 2879
AjnI	CCWGG	9 cut(s)	61, 262, 875, 1218, 1398, 2181, 2494, 2598, 2818
AjuI	GAANNNNNNNTTGG	2 cut(s)	2007, 2039
Alw21I	GWGCWC	6 cut(s)	1150, 1464, 1854, 2037, 2128, 2787
Alw26I	GTCTC	9 cut(s)	413, 728, 1113, 1330, 1523, 1959, 1961, 2484, 2644
Alw44I	GTGCAC	1 cut(s)	2124
Ama87I	CYCGRG	3 cut(s)	1184, 1406, 1427
AoxI	GGCC	7 cut(s)	312, 1402, 1424, 1572, 1807, 1900, 2438
ApaLI	GTGCAC	1 cut(s)	2124
ApeKI	GCWGC	9 cut(s)	653, 929, 1700, 1709, 1820, 2069, 2369, 2777, 2861
ApoI	RAATTY	4 cut(s)	465, 758, 1074, 1512
ArsI	GACNNNNNNTTYG	4 cut(s)	481, 513, 2643, 2675
AsiGI	ACCGGT	2 cut(s)	1242, 2879
Asp700I	GAANNNNTTC	3 cut(s)	330, 810, 2295
AspLEI	GCGC	3 cut(s)	1013, 1225, 1476
AspS9I	GGNCC	7 cut(s)	214, 1402, 1425, 1572, 1931, 2325, 2565
AsuHPI	GGTGA	6 cut(s)	278, 1121, 1274, 1879, 2015, 2446
AsuNHI	GCTAGC	1 cut(s)	1684
AvaI	CYCGRG	3 cut(s)	1184, 1406, 1427
AvaII	GGWCC	4 cut(s)	214, 1931, 2325, 2565
BaeGI	GKGCMC	2 cut(s)	2128, 2613
BalI	TGGCCA	1 cut(s)	314
BamHI	GGATCC	1 cut(s)	259
BanI	GGYRCC	2 cut(s)	58, 1222
BanII	GRGCYC	3 cut(s)	1464, 1854, 2787
BbrPI	CACGTG	2 cut(s)	899, 1139
BbsI	GAAGAC	1 cut(s)	1023
Bbv12I	GWGCWC	6 cut(s)	1150, 1464, 1854, 2037, 2128, 2787
BbvI	GCAGC	9 cut(s)	640, 916, 1687, 1696, 1832, 2081, 2381, 2789, 2848
BccI	CCATC	9 cut(s)	19, 844, 860, 919, 1609, 2134, 2221, 2269, 2467
BceAI	ACGGC	3 cut(s)	1364, 1626, 1794
BcgI	CGANNNNNNTGC	2 cut(s)	83, 117
BciT130I	CCWGG	9 cut(s)	63, 264, 877, 1220, 1400, 2183, 2496, 2600, 2820
BcoDI	GTCTC	9 cut(s)	413, 728, 1113, 1330, 1523, 1959, 1961, 2484, 2644
BfaI	CTAG	7 cut(s)	558, 918, 1340, 1685, 2054, 2078, 2825
BfmI	CTRYAG	2 cut(s)	492, 1818
BfoI	RGCGCY	2 cut(s)	1226, 1477
BfrI	CTTAAG	1 cut(s)	2641
BglI	GCCNNNNNGGC	2 cut(s)	320, 2119
BglII	AGATCT	2 cut(s)	536, 2617
BisI	GCNGC	9 cut(s)	654, 930, 1701, 1710, 1821, 2070, 2370, 2778, 2862
BlsI	GCNGC	9 cut(s)	655, 931, 1702, 1711, 1822, 2071, 2371, 2779, 2863
BmcAI	AGTACT	1 cut(s)	2204
Bme1390I	CCNGG	9 cut(s)	63, 264, 877, 1220, 1400, 2183, 2496, 2600, 2820
Bme18I	GGWCC	4 cut(s)	214, 1931, 2325, 2565
BmeT110I	CYCGRG	3 cut(s)	1184, 1406, 1427
BmgT120I	GGNCC	7 cut(s)	214, 1402, 1425, 1572, 1931, 2325, 2565
BmrFI	CCNGG	9 cut(s)	63, 264, 877, 1220, 1400, 2183, 2496, 2600, 2820
BmrI	ACTGGG	1 cut(s)	2093
BmsI	GCATC	9 cut(s)	28, 91, 1372, 1792, 1837, 2295, 2386, 2403, 2859
BmtI	GCTAGC	1 cut(s)	1688
BmuI	ACTGGG	1 cut(s)	2093
BpiI	GAAGAC	1 cut(s)	1023
BplI	GAGNNNNNCTC	4 cut(s)	1110, 1142, 1869, 1901
BpmI	CTGGAG	3 cut(s)	870, 2582, 2802
BpuEI	CTTGAG	3 cut(s)	681, 1448, 1827
BsaAI	YACGTR	2 cut(s)	899, 1139
BsaBI	GATNNNNATC	2 cut(s)	42, 870
BsaHI	GRCGYC	1 cut(s)	1223
BsaI	GGTCTC	1 cut(s)	2484
BsaWI	WCCGGW	4 cut(s)	1242, 1749, 2221, 2879
BsaXI	ACNNNNNCTCC	6 cut(s)	363, 393, 2024, 2054, 2216, 2246
Bsc4I	CCNNNNNNNGG	4 cut(s)	955, 1484, 2334, 2599
Bse118I	RCCGGY	3 cut(s)	1242, 2761, 2879
Bse1I	ACTGG	3 cut(s)	93, 2099, 2348
Bse8I	GATNNNNATC	2 cut(s)	42, 870
BseBI	CCWGG	9 cut(s)	63, 264, 877, 1220, 1400, 2183, 2496, 2600, 2820
BseGI	GGATG	7 cut(s)	85, 514, 855, 1807, 2140, 2247, 2874
BseJI	GATNNNNATC	2 cut(s)	42, 870
BseLI	CCNNNNNNNGG	4 cut(s)	955, 1484, 2334, 2599
BseMII	CTCAG	9 cut(s)	38, 66, 172, 536, 1449, 1572, 1956, 1970, 2552
BseNI	ACTGG	3 cut(s)	93, 2099, 2348
BseRI	GAGGAG	3 cut(s)	675, 1938, 2045
BseSI	GKGCMC	2 cut(s)	2128, 2613
BseXI	GCAGC	9 cut(s)	640, 916, 1687, 1696, 1832, 2081, 2381, 2789, 2848
BseYI	CCCAGC	2 cut(s)	2606, 2864
Bsh1236I	CGCG	2 cut(s)	194, 1011
BshFI	GGCC	7 cut(s)	314, 1404, 1426, 1574, 1809, 1902, 2440
BshNI	GGYRCC	2 cut(s)	58, 1222
BshTI	ACCGGT	2 cut(s)	1242, 2879
BsiHKAI	GWGCWC	6 cut(s)	1150, 1464, 1854, 2037, 2128, 2787
BsiHKCI	CYCGRG	3 cut(s)	1184, 1406, 1427
BsiSI	CCGG	5 cut(s)	1243, 1750, 2222, 2762, 2880
BslFI	GGGAC	2 cut(s)	439, 1944
BslI	CCNNNNNNNGG	4 cut(s)	955, 1484, 2334, 2599
BsmAI	GTCTC	9 cut(s)	413, 728, 1113, 1330, 1523, 1959, 1961, 2484, 2644
BsmBI	CGTCTC	3 cut(s)	413, 728, 1961
BsmFI	GGGAC	2 cut(s)	439, 1944
BsmI	GAATGC	1 cut(s)	1643
BsnI	GGCC	7 cut(s)	314, 1404, 1426, 1574, 1809, 1902, 2440
Bso31I	GGTCTC	1 cut(s)	2484
BsoBI	CYCGRG	3 cut(s)	1184, 1406, 1427
Bsp1286I	GDGCHC	7 cut(s)	1150, 1464, 1854, 2037, 2128, 2613, 2787
Bsp19I	CCATGG	1 cut(s)	2309
BspACI	CCGC	3 cut(s)	192, 194, 510
BspANI	GGCC	7 cut(s)	314, 1404, 1426, 1574, 1809, 1902, 2440
BspCNI	CTCAG	9 cut(s)	39, 67, 171, 535, 1450, 1571, 1957, 1969, 2553
BspFNI	CGCG	2 cut(s)	194, 1011
BspHI	TCATGA	3 cut(s)	730, 1015, 1773
BspMAI	CTGCAG	1 cut(s)	1822
BspOI	GCTAGC	1 cut(s)	1688
BspQI	GCTCTTC	2 cut(s)	782, 1842
BspT107I	GGYRCC	2 cut(s)	58, 1222
BspTI	CTTAAG	1 cut(s)	2641
BspTNI	GGTCTC	1 cut(s)	2484
BsrFI	RCCGGY	3 cut(s)	1242, 2761, 2879
BsrI	ACTGG	3 cut(s)	93, 2099, 2348
BssAI	RCCGGY	3 cut(s)	1242, 2761, 2879
BssNI	GRCGYC	1 cut(s)	1223
BssT1I	CCWWGG	3 cut(s)	591, 2309, 2484
Bst2UI	CCWGG	9 cut(s)	63, 264, 877, 1220, 1400, 2183, 2496, 2600, 2820
Bst4CI	ACNGT	8 cut(s)	169, 179, 493, 1236, 1251, 1915, 1996, 2168
Bst6I	CTCTTC	5 cut(s)	411, 782, 1730, 1842, 1915
BstACI	GRCGYC	1 cut(s)	1223
BstAFI	CTTAAG	1 cut(s)	2641
BstAPI	GCANNNNNTGC	1 cut(s)	2150
BstBAI	YACGTR	2 cut(s)	899, 1139
BstDSI	CCRYGG	2 cut(s)	192, 2309
BstEII	GGTNACC	1 cut(s)	1262
BstENI	CCTNNNNNAGG	1 cut(s)	2597
BstF5I	GGATG	7 cut(s)	85, 514, 855, 1807, 2140, 2247, 2874
BstFNI	CGCG	2 cut(s)	194, 1011
BstH2I	RGCGCY	2 cut(s)	1226, 1477
BstHHI	GCGC	3 cut(s)	1013, 1225, 1476
BstMAI	GTCTC	9 cut(s)	413, 728, 1113, 1330, 1523, 1959, 1961, 2484, 2644
BstNI	CCWGG	9 cut(s)	63, 264, 877, 1220, 1400, 2183, 2496, 2600, 2820
BstNSI	RCATGY	3 cut(s)	304, 557, 2428
BstPI	GGTNACC	1 cut(s)	1262
BstSCI	CCNGG	9 cut(s)	61, 262, 875, 1218, 1398, 2181, 2494, 2598, 2818
BstSFI	CTRYAG	2 cut(s)	492, 1818
BstSLI	GKGCMC	2 cut(s)	2128, 2613
BstUI	CGCG	2 cut(s)	194, 1011
BstV1I	GCAGC	9 cut(s)	640, 916, 1687, 1696, 1832, 2081, 2381, 2789, 2848
BstV2I	GAAGAC	1 cut(s)	1023
BstX2I	RGATCY	8 cut(s)	154, 259, 536, 958, 2313, 2617, 2632, 2715
BstXI	CCANNNNNNTGG	1 cut(s)	2606
BstYI	RGATCY	8 cut(s)	154, 259, 536, 958, 2313, 2617, 2632, 2715
BsuRI	GGCC	7 cut(s)	314, 1404, 1426, 1574, 1809, 1902, 2440
BtgI	CCRYGG	2 cut(s)	192, 2309
BtsCI	GGATG	7 cut(s)	85, 514, 855, 1807, 2140, 2247, 2874
BtsI	GCAGTG	1 cut(s)	777
BtsIMutI	CAGTG	7 cut(s)	777, 1247, 1368, 1488, 1920, 2355, 2441
CciI	TCATGA	3 cut(s)	730, 1015, 1773
CfoI	GCGC	3 cut(s)	1013, 1225, 1476
Cfr10I	RCCGGY	3 cut(s)	1242, 2761, 2879
Cfr13I	GGNCC	7 cut(s)	214, 1402, 1425, 1572, 1931, 2325, 2565
Cfr42I	CCGCGG	1 cut(s)	195
CseI	GACGC	1 cut(s)	586
Csp6I	GTAC	5 cut(s)	752, 940, 1610, 1741, 2203
CspAI	ACCGGT	2 cut(s)	1242, 2879
CviQI	GTAC	5 cut(s)	752, 940, 1610, 1741, 2203
DinI	GGCGCC	1 cut(s)	1224
DrdI	GACNNNNNNGTC	1 cut(s)	89
DseDI	GACNNNNNNGTC	1 cut(s)	89
EaeI	YGGCCR	3 cut(s)	312, 1807, 1900
Eam1104I	CTCTTC	5 cut(s)	411, 782, 1730, 1842, 1915
EarI	CTCTTC	5 cut(s)	411, 782, 1730, 1842, 1915
Ecl136II	GAGCTC	3 cut(s)	1462, 1852, 2785
Eco130I	CCWWGG	3 cut(s)	591, 2309, 2484
Eco24I	GRGCYC	3 cut(s)	1464, 1854, 2787
Eco31I	GGTCTC	1 cut(s)	2484
Eco47I	GGWCC	4 cut(s)	214, 1931, 2325, 2565
Eco53kI	GAGCTC	3 cut(s)	1462, 1852, 2785
Eco57I	CTGAAG	7 cut(s)	119, 345, 1527, 1539, 2393, 2431, 2729
Eco72I	CACGTG	2 cut(s)	899, 1139
Eco88I	CYCGRG	3 cut(s)	1184, 1406, 1427
Eco91I	GGTNACC	1 cut(s)	1262
EcoICRI	GAGCTC	3 cut(s)	1462, 1852, 2785
EcoNI	CCTNNNNNAGG	1 cut(s)	2597
EcoO109I	RGGNCCY	2 cut(s)	214, 1572
EcoO65I	GGTNACC	1 cut(s)	1262
EcoRII	CCWGG	9 cut(s)	61, 262, 875, 1218, 1398, 2181, 2494, 2598, 2818
EcoT14I	CCWWGG	3 cut(s)	591, 2309, 2484
EcoT38I	GRGCYC	3 cut(s)	1464, 1854, 2787
EgeI	GGCGCC	1 cut(s)	1224
EheI	GGCGCC	1 cut(s)	1224
ErhI	CCWWGG	3 cut(s)	591, 2309, 2484
Esp3I	CGTCTC	3 cut(s)	413, 728, 1961
FaqI	GGGAC	2 cut(s)	439, 1944
Fnu4HI	GCNGC	9 cut(s)	654, 930, 1701, 1710, 1821, 2070, 2370, 2778, 2862
FokI	GGATG	7 cut(s)	92, 501, 862, 1814, 2147, 2254, 2881
FriOI	GRGCYC	3 cut(s)	1464, 1854, 2787
Fsp4HI	GCNGC	9 cut(s)	654, 930, 1701, 1710, 1821, 2070, 2370, 2778, 2862
FspBI	CTAG	7 cut(s)	558, 918, 1340, 1685, 2054, 2078, 2825
GlaI	GCGC	3 cut(s)	1012, 1224, 1475
GluI	GCNGC	9 cut(s)	654, 930, 1701, 1710, 1821, 2070, 2370, 2778, 2862
GsaI	CCCAGC	2 cut(s)	2610, 2868
GsuI	CTGGAG	3 cut(s)	870, 2582, 2802
HaeII	RGCGCY	2 cut(s)	1226, 1477
HaeIII	GGCC	7 cut(s)	314, 1404, 1426, 1574, 1809, 1902, 2440
HapII	CCGG	5 cut(s)	1243, 1750, 2222, 2762, 2880
HgaI	GACGC	1 cut(s)	586
HhaI	GCGC	3 cut(s)	1013, 1225, 1476
Hin1I	GRCGYC	1 cut(s)	1223
Hin6I	GCGC	3 cut(s)	1011, 1223, 1474
HinP1I	GCGC	3 cut(s)	1011, 1223, 1474
HincII	GTYRAC	2 cut(s)	249, 775
HindII	GTYRAC	2 cut(s)	249, 775
HindIII	AAGCTT	3 cut(s)	431, 2519, 2792
HpaI	GTTAAC	1 cut(s)	249
HpaII	CCGG	5 cut(s)	1243, 1750, 2222, 2762, 2880
HphI	GGTGA	6 cut(s)	278, 1121, 1274, 1879, 2015, 2446
Hpy166II	GTNNAC	5 cut(s)	249, 752, 775, 1142, 2126
Hpy8I	GTNNAC	5 cut(s)	249, 752, 775, 1142, 2126
Hpy99I	CGWCG	2 cut(s)	671, 1283
HpyAV	CCTTC	9 cut(s)	61, 197, 205, 345, 361, 672, 1298, 2621, 2845
HpyCH4III	ACNGT	8 cut(s)	169, 179, 493, 1236, 1251, 1915, 1996, 2168
HpyCH4IV	ACGT	7 cut(s)	422, 808, 898, 1138, 1144, 1303, 1633
HpySE526I	ACGT	7 cut(s)	422, 808, 898, 1138, 1144, 1303, 1633
Hsp92I	GRCGYC	1 cut(s)	1223
HspAI	GCGC	3 cut(s)	1011, 1223, 1474
KasI	GGCGCC	1 cut(s)	1222
KspAI	GTTAAC	1 cut(s)	249
KspI	CCGCGG	1 cut(s)	195
LguI	GCTCTTC	2 cut(s)	782, 1842
LmnI	GCTCC	6 cut(s)	5, 1284, 1708, 1989, 2032, 2821
Lsp1109I	GCAGC	9 cut(s)	640, 916, 1687, 1696, 1832, 2081, 2381, 2789, 2848
LweI	GCATC	9 cut(s)	28, 91, 1372, 1792, 1837, 2295, 2386, 2403, 2859
MaeI	CTAG	7 cut(s)	558, 918, 1340, 1685, 2054, 2078, 2825
MaeII	ACGT	7 cut(s)	422, 808, 898, 1138, 1144, 1303, 1633
MfeI	CAATTG	2 cut(s)	33, 1272
MflI	RGATCY	8 cut(s)	154, 259, 536, 958, 2313, 2617, 2632, 2715
MhlI	GDGCHC	7 cut(s)	1150, 1464, 1854, 2037, 2128, 2613, 2787
MlsI	TGGCCA	1 cut(s)	314
MluNI	TGGCCA	1 cut(s)	314
Mly113I	GGCGCC	1 cut(s)	1223
MlyI	GAGTC	6 cut(s)	263, 413, 676, 2263, 2460, 2660
MmeI	TCCRAC	3 cut(s)	93, 387, 713
Mox20I	TGGCCA	1 cut(s)	314
MroXI	GAANNNNTTC	3 cut(s)	330, 810, 2295
MscI	TGGCCA	1 cut(s)	314
MseI	TTAA	7 cut(s)	248, 306, 1881, 2376, 2465, 2642, 2796
MslI	CAYNNNNRTG	1 cut(s)	1371
Msp20I	TGGCCA	1 cut(s)	314
MspA1I	CMGCKG	1 cut(s)	194
MspCI	CTTAAG	1 cut(s)	2641
MspI	CCGG	5 cut(s)	1243, 1750, 2222, 2762, 2880
MspR9I	CCNGG	9 cut(s)	63, 264, 877, 1220, 1400, 2183, 2496, 2600, 2820
MunI	CAATTG	2 cut(s)	33, 1272
Mva1269I	GAATGC	1 cut(s)	1643
MvaI	CCWGG	9 cut(s)	63, 264, 877, 1220, 1400, 2183, 2496, 2600, 2820
MvnI	CGCG	2 cut(s)	194, 1011
NarI	GGCGCC	1 cut(s)	1223
NcoI	CCATGG	1 cut(s)	2309
NheI	GCTAGC	1 cut(s)	1684
NmeAIII	GCCGAG	2 cut(s)	794, 1928
NmuCI	GTSAC	9 cut(s)	266, 880, 1245, 1262, 2420, 2452, 2529, 2663, 2720
NspI	RCATGY	3 cut(s)	304, 557, 2428
PaeI	GCATGC	1 cut(s)	557
PagI	TCATGA	3 cut(s)	730, 1015, 1773
PasI	CCCWGGG	1 cut(s)	876
PciI	ACATGT	1 cut(s)	2424
PciSI	GCTCTTC	2 cut(s)	782, 1842
PcsI	WCGNNNNNNNCGW	1 cut(s)	1639
PctI	GAATGC	1 cut(s)	1643
PdmI	GAANNNNTTC	3 cut(s)	330, 810, 2295
PfeI	GAWTC	7 cut(s)	646, 1355, 1790, 1972, 2092, 2291, 2404
PfoI	TCCNGGA	1 cut(s)	2181
PinAI	ACCGGT	2 cut(s)	1242, 2879
PkrI	GCNGC	9 cut(s)	655, 931, 1702, 1711, 1822, 2071, 2371, 2779, 2863
PleI	GAGTC	6 cut(s)	263, 412, 676, 2262, 2459, 2660
PluTI	GGCGCC	1 cut(s)	1226
PmaCI	CACGTG	2 cut(s)	899, 1139
PmlI	CACGTG	2 cut(s)	899, 1139
PpsI	GAGTC	6 cut(s)	263, 412, 676, 2262, 2459, 2660
Ppu21I	YACGTR	2 cut(s)	899, 1139
PpuMI	RGGWCCY	1 cut(s)	214
PscI	ACATGT	1 cut(s)	2424
Psp124BI	GAGCTC	3 cut(s)	1464, 1854, 2787
Psp5II	RGGWCCY	1 cut(s)	214
Psp6I	CCWGG	9 cut(s)	61, 262, 875, 1218, 1398, 2181, 2494, 2598, 2818
PspCI	CACGTG	2 cut(s)	899, 1139
PspEI	GGTNACC	1 cut(s)	1262
PspFI	CCCAGC	2 cut(s)	2606, 2864
PspGI	CCWGG	9 cut(s)	61, 262, 875, 1218, 1398, 2181, 2494, 2598, 2818
PspPI	GGNCC	7 cut(s)	214, 1402, 1425, 1572, 1931, 2325, 2565
PspPPI	RGGWCCY	1 cut(s)	214
PsrI	GAACNNNNNNTAC	2 cut(s)	486, 518
PstI	CTGCAG	1 cut(s)	1822
PsuI	RGATCY	8 cut(s)	154, 259, 536, 958, 2313, 2617, 2632, 2715
RsaI	GTAC	5 cut(s)	753, 941, 1611, 1742, 2204
RsaNI	GTAC	5 cut(s)	752, 940, 1610, 1741, 2203
RseI	CAYNNNNRTG	1 cut(s)	1371
SacI	GAGCTC	3 cut(s)	1464, 1854, 2787
SacII	CCGCGG	1 cut(s)	195
SapI	GCTCTTC	2 cut(s)	782, 1842
SaqAI	TTAA	7 cut(s)	248, 306, 1881, 2376, 2465, 2642, 2796
SatI	GCNGC	9 cut(s)	654, 930, 1701, 1710, 1821, 2070, 2370, 2778, 2862
Sau96I	GGNCC	7 cut(s)	214, 1402, 1425, 1572, 1931, 2325, 2565
ScaI	AGTACT	1 cut(s)	2204
SchI	GAGTC	6 cut(s)	263, 413, 676, 2263, 2460, 2660
ScrFI	CCNGG	9 cut(s)	63, 264, 877, 1220, 1400, 2183, 2496, 2600, 2820
SduI	GDGCHC	7 cut(s)	1150, 1464, 1854, 2037, 2128, 2613, 2787
SfaNI	GCATC	9 cut(s)	28, 91, 1372, 1792, 1837, 2295, 2386, 2403, 2859
SfcI	CTRYAG	2 cut(s)	492, 1818
SfoI	GGCGCC	1 cut(s)	1224
Sfr303I	CCGCGG	1 cut(s)	195
SgrBI	CCGCGG	1 cut(s)	195
SinI	GGWCC	4 cut(s)	214, 1931, 2325, 2565
SmiMI	CAYNNNNRTG	1 cut(s)	1371
SmlI	CTYRAG	4 cut(s)	696, 1463, 1842, 2641
SmoI	CTYRAG	4 cut(s)	696, 1463, 1842, 2641
SphI	GCATGC	1 cut(s)	557
SsiI	CCGC	3 cut(s)	192, 194, 510
SspDI	GGCGCC	1 cut(s)	1222
SspMI	CTAG	7 cut(s)	558, 918, 1340, 1685, 2054, 2078, 2825
SstI	GAGCTC	3 cut(s)	1464, 1854, 2787
StyD4I	CCNGG	9 cut(s)	61, 262, 875, 1218, 1398, 2181, 2494, 2598, 2818
StyI	CCWWGG	3 cut(s)	591, 2309, 2484
TaaI	ACNGT	8 cut(s)	169, 179, 493, 1236, 1251, 1915, 1996, 2168
TaiI	ACGT	7 cut(s)	425, 811, 901, 1141, 1147, 1306, 1636
TaqI	TCGA	8 cut(s)	93, 126, 471, 624, 1230, 1417, 2289, 2299
TatI	WGTACW	3 cut(s)	939, 1609, 2202
TfiI	GAWTC	7 cut(s)	646, 1355, 1790, 1972, 2092, 2291, 2404
Tru1I	TTAA	7 cut(s)	248, 306, 1881, 2376, 2465, 2642, 2796
Tru9I	TTAA	7 cut(s)	248, 306, 1881, 2376, 2465, 2642, 2796
TscAI	CASTG	7 cut(s)	784, 1254, 1375, 1495, 1920, 2355, 2441
TseFI	GTSAC	9 cut(s)	266, 880, 1245, 1262, 2420, 2452, 2529, 2663, 2720
TseI	GCWGC	9 cut(s)	653, 929, 1700, 1709, 1820, 2069, 2369, 2777, 2861
Tsp45I	GTSAC	9 cut(s)	266, 880, 1245, 1262, 2420, 2452, 2529, 2663, 2720
TspGWI	ACGGA	4 cut(s)	416, 1976, 2171, 2708
TspRI	CASTG	7 cut(s)	784, 1254, 1375, 1495, 1920, 2355, 2441
Vha464I	CTTAAG	1 cut(s)	2641
VneI	GTGCAC	1 cut(s)	2124
VpaK11BI	GGWCC	4 cut(s)	214, 1931, 2325, 2565
XagI	CCTNNNNNAGG	1 cut(s)	2597
XapI	RAATTY	4 cut(s)	465, 758, 1074, 1512
XbaI	TCTAGA	1 cut(s)	2824
XceI	RCATGY	3 cut(s)	304, 557, 2428
XmnI	GAANNNNTTC	3 cut(s)	330, 810, 2295
XspI	CTAG	7 cut(s)	558, 918, 1340, 1685, 2054, 2078, 2825
ZrmI	AGTACT	1 cut(s)	2204

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.