FvH4_6g27820

Belongs to the peroxisomal membrane protein PXMP2 4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011150
Physical Location & Seq
Reverse (-)
21429413 .. 21432463
3051 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27820.t2

Sequence Viewer

Length: 672 bp
ATGCATGCTTTGGGAGGTGGAGGTTATGGCGGGTTCTGGGGATGGGACTCAATACCAAAGAAACCCAAACAACAAAGAAAGCGATTCGATTCCAAATCCTCCAGTTCTGTCGAGGCCACCGGAGGACCCGGTTTCCGGTTCCCGTTAAAGCAAGCAGTGACGGCTGGCTCACTGGCTTTAACCGGAGACACTATTGCTCAGCTCAGAGAGCGCTGGAGAAAGGCCAGGACTTTAGACTCCAACCCCACCTCTCAGGATGTCACTCAAACTCTCCTCTCAGACCATGATTGGCTTCGTGCACTTCGGATGACTTCTTATGGGTTTCTGCTGTACGGCCCTGGCTCTTATGCTTGGTACCAGTATCTTGATCATTCTTTGCCAGGGAAAACTGTAGTGAACCTAATTCTGAAGGTTTTCTTAAACCAAGTTGTTCTTGGTCCATCTGTGATTGCTGTTGTTTTTGCATGGAACAATATATGGCAAGGGAAACTCTCACAGCTTTCGGAAAAGTACCGGAGAGATGCTCTTCCCACATTACTTTACGGATTTAGGTTTTGGATACCTGTCAGCTTATTAAACTTCTGGGTGGTGCCTCTTCAGGCCCGTGTAGCTTTCATGTGTGTGGGTTCAATATTCTGGAACTTCTGCTTGTCTTCGACAATGAGCAAGTAA

Protein Analysis

224

Amino Acids

25.12

Weight (kDa)

10.0

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpv17_PMP22 PF04117 164 - 223 5.3e-16 Mpv17 / PMP22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 354
AccB1I GGYRCC 2 cut(s) 354, 589
AciI CCGC 1 cut(s) 30
AcuI CTGAAG 2 cut(s) 428, 581
AfaI GTAC 3 cut(s) 332, 356, 512
AfeI AGCGCT 1 cut(s) 212
AfiI CCNNNNNNNGG 1 cut(s) 135
AgsI TTSAA 1 cut(s) 630
AjnI CCWGG 3 cut(s) 224, 337, 379
AluBI AGCT 4 cut(s) 202, 499, 570, 611
AluI AGCT 4 cut(s) 202, 499, 570, 611
Alw21I GWGCWC 1 cut(s) 301
Alw26I GTCTC 1 cut(s) 180
Alw44I GTGCAC 1 cut(s) 297
Aor51HI AGCGCT 1 cut(s) 212
AoxI GGCC 4 cut(s) 114, 222, 334, 600
ApaLI GTGCAC 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 413
Asp718I GGTACC 1 cut(s) 354
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 4 cut(s) 125, 335, 437, 601
AsuC2I CCSGG 1 cut(s) 129
AvaII GGWCC 2 cut(s) 125, 437
BaeGI GKGCMC 1 cut(s) 301
BanI GGYRCC 2 cut(s) 354, 589
BbsI GAAGAC 1 cut(s) 645
Bbv12I GWGCWC 1 cut(s) 301
BccI CCATC 2 cut(s) 36, 448
BceAI ACGGC 2 cut(s) 177, 349
BciT130I CCWGG 3 cut(s) 226, 339, 381
BciVI GTATCC 1 cut(s) 552
BclI TGATCA 1 cut(s) 367
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 180
BfmI CTRYAG 1 cut(s) 390
BfoI RGCGCY 1 cut(s) 214
BfuI GTATCC 1 cut(s) 552
BlpI GCTNAGC 1 cut(s) 198
Bme1390I CCNGG 4 cut(s) 129, 226, 339, 381
Bme18I GGWCC 2 cut(s) 125, 437
BmgT120I GGNCC 4 cut(s) 125, 335, 437, 601
BmiI GGNNCC 4 cut(s) 127, 140, 356, 591
BmrFI CCNGG 4 cut(s) 129, 226, 339, 381
BmsI GCATC 1 cut(s) 511
BpiI GAAGAC 1 cut(s) 645
BplI GAGNNNNNCTC 2 cut(s) 508, 540
BpmI CTGGAG 2 cut(s) 85, 235
Bpu1102I GCTNAGC 1 cut(s) 198
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 337, 380
BsaWI WCCGGW 4 cut(s) 119, 135, 182, 513
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse1I ACTGG 3 cut(s) 102, 177, 358
BseBI CCWGG 3 cut(s) 226, 339, 381
BseDI CCNNGG 2 cut(s) 337, 380
BseGI GGATG 3 cut(s) 47, 262, 312
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMII CTCAG 4 cut(s) 212, 217, 266, 291
BseNI ACTGG 3 cut(s) 102, 177, 358
BseRI GAGGAG 1 cut(s) 263
BseSI GKGCMC 1 cut(s) 301
BshFI GGCC 4 cut(s) 116, 224, 336, 602
BshNI GGYRCC 2 cut(s) 354, 589
BsiHKAI GWGCWC 1 cut(s) 301
BsiSI CCGG 5 cut(s) 120, 129, 136, 183, 514
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 135
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 59
BsnI GGCC 4 cut(s) 116, 224, 336, 602
Bsp1286I GDGCHC 1 cut(s) 301
Bsp143I GATC 1 cut(s) 367
Bsp1720I GCTNAGC 1 cut(s) 198
BspACI CCGC 1 cut(s) 30
BspANI GGCC 4 cut(s) 116, 224, 336, 602
BspCNI CTCAG 4 cut(s) 211, 216, 265, 290
BspLI GGNNCC 4 cut(s) 127, 140, 356, 591
BspQI GCTCTTC 1 cut(s) 531
BspT107I GGYRCC 2 cut(s) 354, 589
BsrI ACTGG 3 cut(s) 102, 177, 358
BssECI CCNNGG 2 cut(s) 337, 380
BssMI GATC 1 cut(s) 367
Bst2UI CCWGG 3 cut(s) 226, 339, 381
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 2 cut(s) 531, 600
BstC8I GCNNGC 3 cut(s) 6, 153, 166
BstDEI CTNAG 4 cut(s) 198, 203, 252, 277
BstF5I GGATG 3 cut(s) 47, 262, 312
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 1 cut(s) 370
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 1 cut(s) 367
BstMWI GCNNNNNNNGC 3 cut(s) 161, 208, 608
BstNI CCWGG 3 cut(s) 226, 339, 381
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 4 cut(s) 127, 224, 337, 379
BstSFI CTRYAG 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 301
BstV2I GAAGAC 1 cut(s) 645
BsuI GTATCC 1 cut(s) 552
BsuRI GGCC 4 cut(s) 116, 224, 336, 602
BtsCI GGATG 3 cut(s) 47, 262, 312
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 162, 170
Cac8I GCNNGC 3 cut(s) 6, 153, 166
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 4 cut(s) 125, 335, 437, 601
Csp6I GTAC 3 cut(s) 331, 355, 511
CviAII CATG 4 cut(s) 5, 284, 465, 616
CviQI GTAC 3 cut(s) 331, 355, 511
DdeI CTNAG 4 cut(s) 198, 203, 252, 277
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
Eam1104I CTCTTC 2 cut(s) 531, 600
EarI CTCTTC 2 cut(s) 531, 600
Eco47I GGWCC 2 cut(s) 125, 437
Eco47III AGCGCT 1 cut(s) 212
Eco57I CTGAAG 2 cut(s) 428, 581
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 3 cut(s) 224, 337, 379
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 4 cut(s) 8, 287, 468, 619
FaiI YATR 9 cut(s) 6, 27, 285, 318, 348, 466, 476, 478, 617
FalI AAGNNNNNCTT 4 cut(s) 401, 433, 417, 449
FaqI GGGAC 1 cut(s) 59
FatI CATG 4 cut(s) 4, 283, 464, 615
FauI CCCGC 1 cut(s) 23
FbaI TGATCA 1 cut(s) 367
FokI GGATG 3 cut(s) 54, 269, 319
GlaI GCGC 1 cut(s) 212
GsuI CTGGAG 2 cut(s) 85, 235
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 4 cut(s) 116, 224, 336, 602
HapII CCGG 5 cut(s) 120, 129, 136, 183, 514
HhaI GCGC 1 cut(s) 213
Hin1II CATG 4 cut(s) 8, 287, 468, 619
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HinfI GANTC 4 cut(s) 47, 84, 89, 236
HpaII CCGG 5 cut(s) 120, 129, 136, 183, 514
Hpy166II GTNNAC 2 cut(s) 299, 397
Hpy188I TCNGA 5 cut(s) 206, 280, 306, 408, 505
Hpy188III TCNNGA 3 cut(s) 254, 365, 637
Hpy8I GTNNAC 2 cut(s) 299, 397
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 3 cut(s) 4, 299, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 208, 608
HpyF3I CTNAG 4 cut(s) 198, 203, 252, 277
Hsp92II CATG 4 cut(s) 8, 287, 468, 619
HspAI GCGC 1 cut(s) 211
KpnI GGTACC 1 cut(s) 358
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 1 cut(s) 367
LguI GCTCTTC 1 cut(s) 531
LweI GCATC 1 cut(s) 511
MaeIII GTNAC 2 cut(s) 157, 259
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MboII GAAGA 3 cut(s) 518, 587, 645
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 1 cut(s) 402
MlyI GAGTC 2 cut(s) 41, 230
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 8 cut(s) 8, 14, 106, 109, 116, 259, 284, 603
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 413
MseI TTAA 4 cut(s) 146, 179, 419, 575
MslI CAYNNNNRTG 1 cut(s) 620
MspI CCGG 5 cut(s) 120, 129, 136, 183, 514
MspR9I CCNGG 4 cut(s) 129, 226, 339, 381
MvaI CCWGG 3 cut(s) 226, 339, 381
MwoI GCNNNNNNNGC 3 cut(s) 161, 208, 608
NciI CCSGG 1 cut(s) 129
NdeII GATC 1 cut(s) 367
NlaIII CATG 4 cut(s) 8, 287, 468, 619
NlaIV GGNNCC 4 cut(s) 127, 140, 356, 591
NmuCI GTSAC 2 cut(s) 157, 259
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 531
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 2 cut(s) 84, 89
PleI GAGTC 2 cut(s) 41, 230
PpsI GAGTC 2 cut(s) 41, 230
PpuMI RGGWCCY 1 cut(s) 125
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 3 cut(s) 224, 337, 379
PspGI CCWGG 3 cut(s) 224, 337, 379
PspN4I GGNNCC 4 cut(s) 127, 140, 356, 591
PspPI GGNCC 4 cut(s) 125, 335, 437, 601
PspPPI RGGWCCY 1 cut(s) 125
RsaI GTAC 3 cut(s) 332, 356, 512
RsaNI GTAC 3 cut(s) 331, 355, 511
RseI CAYNNNNRTG 1 cut(s) 620
SapI GCTCTTC 1 cut(s) 531
SaqAI TTAA 4 cut(s) 146, 179, 419, 575
Sau3AI GATC 1 cut(s) 367
Sau96I GGNCC 4 cut(s) 125, 335, 437, 601
SchI GAGTC 2 cut(s) 41, 230
ScrFI CCNGG 4 cut(s) 129, 226, 339, 381
SduI GDGCHC 1 cut(s) 301
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 2 cut(s) 125, 437
SmiMI CAYNNNNRTG 1 cut(s) 620
SphI GCATGC 1 cut(s) 8
Sse9I AATT 1 cut(s) 402
SsiI CCGC 1 cut(s) 30
SspI AATATT 1 cut(s) 633
StyD4I CCNGG 4 cut(s) 127, 224, 337, 379
TaaI ACNGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 87, 111, 656
TasI AATT 1 cut(s) 402
TfiI GAWTC 2 cut(s) 84, 89
Tru1I TTAA 4 cut(s) 146, 179, 419, 575
Tru9I TTAA 4 cut(s) 146, 179, 419, 575
TscAI CASTG 2 cut(s) 162, 177
TseFI GTSAC 2 cut(s) 157, 259
Tsp45I GTSAC 2 cut(s) 157, 259
TspDTI ATGAA 1 cut(s) 604
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 2 cut(s) 162, 177
VneI GTGCAC 1 cut(s) 297
VpaK11BI GGWCC 2 cut(s) 125, 437
XceI RCATGY 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 413
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.