MD17G1263400.v1.1

Belongs to the peroxisomal membrane protein PXMP2 4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011150
Physical Location & Seq
Forward (+)
32441045 .. 32444002
2958 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1263400.v1.1.491

Sequence Viewer

Length: 675 bp
ATGAATGCTCTTGGCGGTGGCGGCCATGGCGGCCTCTGGGGTTGGAACTACTCATCACCCAGAAAACCGAAGCAGCAAAGAAAACGCCCATTTGATTCCAAATCCCAGTCTTCCTCCAAATCTGAGGACGCAACCGGAAGAGCAGGTCACTGGTTTCCGTTAAAGCAAGCCGCCACGGCTGGCACACTGGCTCTGACGGGAGATACAATTGCCCAGCTCACACAGCGCTGGAGAAAAGCAGAGGCGGAAAACCAACAGGATGTAACACATGCTCTCCTCTCACACCATGATTGGCTTCGTGCACTGCGGATGACTTCCTATGGGTTTCTTTTGTACGGCCCTGGTTCTTATGCTTGGTACCAGTATCTTGATCATTCTTTGCCTGCGAAAACTGTAGAGAACCTACTGCTGAAGGTTTTGTTGAATCAAATTGTGCTTGGTCCATGTGTGATTGCTGTTGTTTTTGCATGGAACAATTTATGGCAAGGGAAACTCTCACAGCTTCCAGAGAAGTATCGAATAGATGCTCTTCCCACCTTACTTTATGGATTTAGGTTCTGGATACCTGTCAGTTTATTGAATTTCTGGGTGGTACCTCTTCAAGCTCGTGTAGCTTTCATGTCCGTGGGTTCAATTTTCTGGAACTTCTGCTTGTCTTCAACTATGAGCAAGTAG

Protein Analysis

225

Amino Acids

25.27

Weight (kDa)

9.84

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpv17_PMP22 PF04117 160 - 219 2e-15 Mpv17 / PMP22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 134
Acc65I GGTACC 2 cut(s) 357, 592
AccB1I GGYRCC 2 cut(s) 357, 592
AciI CCGC 6 cut(s) 15, 21, 30, 171, 245, 307
AcoI YGGCCR 1 cut(s) 22
AcsI RAATTY 1 cut(s) 580
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 3 cut(s) 335, 359, 594
AfeI AGCGCT 1 cut(s) 227
AgsI TTSAA 5 cut(s) 424, 580, 602, 633, 660
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 4 cut(s) 217, 502, 605, 614
AluI AGCT 4 cut(s) 217, 502, 605, 614
Alw21I GWGCWC 1 cut(s) 304
Alw44I GTGCAC 1 cut(s) 300
Aor51HI AGCGCT 1 cut(s) 227
AoxI GGCC 3 cut(s) 22, 31, 337
ApaLI GTGCAC 1 cut(s) 300
ApeKI GCWGC 1 cut(s) 73
ApoI RAATTY 1 cut(s) 580
Asp718I GGTACC 2 cut(s) 357, 592
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 2 cut(s) 338, 440
AsuHPI GGTGA 1 cut(s) 48
AvaII GGWCC 1 cut(s) 440
BaeGI GKGCMC 1 cut(s) 304
BanI GGYRCC 2 cut(s) 357, 592
BauI CACGAG 1 cut(s) 606
BbsI GAAGAC 2 cut(s) 102, 648
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 85
BceAI ACGGC 2 cut(s) 192, 352
BciT130I CCWGG 1 cut(s) 342
BciVI GTATCC 1 cut(s) 555
BclI TGATCA 1 cut(s) 370
BfmI CTRYAG 1 cut(s) 393
BfoI RGCGCY 1 cut(s) 229
BfuAI ACCTGC 1 cut(s) 134
BfuI GTATCC 1 cut(s) 555
BglI GCCNNNNNGGC 2 cut(s) 30, 176
BisI GCNGC 4 cut(s) 22, 31, 74, 171
BlsI GCNGC 4 cut(s) 23, 32, 75, 172
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 2 cut(s) 338, 440
BmiI GGNNCC 2 cut(s) 359, 594
BmrFI CCNGG 1 cut(s) 342
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 1 cut(s) 514
BmuI ACTGGG 1 cut(s) 100
BpiI GAAGAC 2 cut(s) 102, 648
BpmI CTGGAG 1 cut(s) 250
BsaJI CCNNGG 4 cut(s) 25, 174, 340, 624
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bse1I ACTGG 4 cut(s) 106, 155, 192, 361
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 4 cut(s) 25, 174, 340, 624
BseGI GGATG 2 cut(s) 265, 315
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 4 cut(s) 106, 155, 192, 361
BseRI GAGGAG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 304
BseXI GCAGC 1 cut(s) 85
BseYI CCCAGC 1 cut(s) 213
BshFI GGCC 3 cut(s) 24, 33, 339
BshNI GGYRCC 2 cut(s) 357, 592
BsiHKAI GWGCWC 1 cut(s) 304
BsiSI CCGG 1 cut(s) 135
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 3 cut(s) 24, 33, 339
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 1 cut(s) 370
Bsp19I CCATGG 1 cut(s) 25
BspACI CCGC 6 cut(s) 15, 21, 30, 171, 245, 307
BspANI GGCC 3 cut(s) 24, 33, 339
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 359, 594
BspMI ACCTGC 1 cut(s) 134
BspQI GCTCTTC 2 cut(s) 133, 534
BspT107I GGYRCC 2 cut(s) 357, 592
BsrI ACTGG 4 cut(s) 106, 155, 192, 361
BssECI CCNNGG 4 cut(s) 25, 174, 340, 624
BssMI GATC 1 cut(s) 370
BssSI CACGAG 1 cut(s) 606
BssT1I CCWWGG 1 cut(s) 25
Bst2BI CACGAG 1 cut(s) 606
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 3 cut(s) 133, 534, 603
BstC8I GCNNGC 3 cut(s) 168, 181, 384
BstDEI CTNAG 1 cut(s) 123
BstDSI CCRYGG 3 cut(s) 25, 174, 624
BstF5I GGATG 2 cut(s) 265, 315
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 1 cut(s) 373
BstMBI GATC 1 cut(s) 370
BstMWI GCNNNNNNNGC 6 cut(s) 21, 27, 30, 176, 223, 611
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 393
BstSLI GKGCMC 1 cut(s) 304
BstV1I GCAGC 1 cut(s) 85
BstV2I GAAGAC 2 cut(s) 102, 648
BsuI GTATCC 1 cut(s) 555
BsuRI GGCC 3 cut(s) 24, 33, 339
BtgI CCRYGG 3 cut(s) 25, 174, 624
BtsCI GGATG 2 cut(s) 265, 315
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 3 cut(s) 148, 185, 302
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 3 cut(s) 168, 181, 384
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 2 cut(s) 338, 440
CseI GACGC 1 cut(s) 137
Csp6I GTAC 3 cut(s) 334, 358, 593
CviAII CATG 6 cut(s) 26, 269, 287, 444, 468, 619
CviQI GTAC 3 cut(s) 334, 358, 593
DdeI CTNAG 1 cut(s) 123
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
EaeI YGGCCR 1 cut(s) 22
Eam1104I CTCTTC 3 cut(s) 133, 534, 603
EarI CTCTTC 3 cut(s) 133, 534, 603
EciI GGCGGA 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 440
Eco47III AGCGCT 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 25
FaeI CATG 6 cut(s) 29, 272, 290, 447, 471, 622
FatI CATG 6 cut(s) 25, 268, 286, 443, 467, 618
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 4 cut(s) 22, 31, 74, 171
FokI GGATG 2 cut(s) 272, 322
Fsp4HI GCNGC 4 cut(s) 22, 31, 74, 171
GlaI GCGC 1 cut(s) 227
GluI GCNGC 4 cut(s) 22, 31, 74, 171
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 250
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 3 cut(s) 24, 33, 339
HapII CCGG 1 cut(s) 135
HgaI GACGC 1 cut(s) 137
HhaI GCGC 1 cut(s) 228
Hin1II CATG 6 cut(s) 29, 272, 290, 447, 471, 622
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 2 cut(s) 95, 424
HpaII CCGG 1 cut(s) 135
HphI GGTGA 1 cut(s) 48
Hpy166II GTNNAC 1 cut(s) 302
Hpy188I TCNGA 2 cut(s) 124, 195
Hpy188III TCNNGA 4 cut(s) 368, 506, 559, 640
Hpy8I GTNNAC 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4V TGCA 2 cut(s) 302, 467
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 27, 30, 176, 223, 611
HpyF3I CTNAG 1 cut(s) 123
Hsp92II CATG 6 cut(s) 29, 272, 290, 447, 471, 622
HspAI GCGC 1 cut(s) 226
KpnI GGTACC 2 cut(s) 361, 596
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 1 cut(s) 370
LguI GCTCTTC 2 cut(s) 133, 534
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 1 cut(s) 514
MaeIII GTNAC 2 cut(s) 146, 262
MalI GATC 1 cut(s) 372
MboI GATC 1 cut(s) 370
MboII GAAGA 5 cut(s) 102, 150, 521, 590, 648
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 5 cut(s) 207, 429, 475, 580, 633
MmeI TCCRAC 1 cut(s) 23
MnlI CCTC 6 cut(s) 44, 118, 124, 235, 287, 606
MseI TTAA 1 cut(s) 161
MslI CAYNNNNRTG 1 cut(s) 623
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 1 cut(s) 342
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 6 cut(s) 21, 27, 30, 176, 223, 611
NcoI CCATGG 1 cut(s) 25
NdeII GATC 1 cut(s) 370
NlaIII CATG 6 cut(s) 29, 272, 290, 447, 471, 622
NlaIV GGNNCC 2 cut(s) 359, 594
NmuCI GTSAC 1 cut(s) 146
NspI RCATGY 1 cut(s) 272
PciSI GCTCTTC 2 cut(s) 133, 534
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 95, 424
PkrI GCNGC 4 cut(s) 23, 32, 75, 172
Psp6I CCWGG 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 359, 594
PspPI GGNCC 2 cut(s) 338, 440
RsaI GTAC 3 cut(s) 335, 359, 594
RsaNI GTAC 3 cut(s) 334, 358, 593
RseI CAYNNNNRTG 1 cut(s) 623
SapI GCTCTTC 2 cut(s) 133, 534
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 4 cut(s) 22, 31, 74, 171
Sau3AI GATC 1 cut(s) 370
Sau96I GGNCC 2 cut(s) 338, 440
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 1 cut(s) 304
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 393
SfiI GGCCNNNNNGGCC 1 cut(s) 30
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 623
Sse9I AATT 5 cut(s) 207, 429, 475, 580, 633
SsiI CCGC 6 cut(s) 15, 21, 30, 171, 245, 307
StyD4I CCNGG 1 cut(s) 340
StyI CCWWGG 1 cut(s) 25
TaaI ACNGT 1 cut(s) 394
TaqI TCGA 1 cut(s) 517
TasI AATT 5 cut(s) 207, 429, 475, 580, 633
TauI GCSGC 3 cut(s) 24, 33, 173
TfiI GAWTC 2 cut(s) 95, 424
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 3 cut(s) 155, 192, 309
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 1 cut(s) 73
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 2 cut(s) 17, 607
TspGWI ACGGA 2 cut(s) 147, 613
TspRI CASTG 3 cut(s) 155, 192, 309
VneI GTGCAC 1 cut(s) 300
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 580
XceI RCATGY 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.