MD09G1234300.v1.1

Belongs to the peroxisomal membrane protein PXMP2 4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011150
Physical Location & Seq
Forward (+)
29452100 .. 29455308
3209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1234300.v1.1.491

Sequence Viewer

Length: 681 bp
ATGAATGCTCTTGGCGGTGGCGGCTATGGCGGCCTCTGGGGTTGGAACTCCTCATCACCCAGAAAACCCAAGCAGCCAAGAAGACGCCCATTCGATTCCAAATCCCAGTCCTCCTCCAATTCCAATTCTGTGGACGCAACCGGAAGAGCCCGTCACTGGTTCCCCTTAATGCAAGCCGCCACGGCTGGCTCACTGGCTCTGACGGGAGATACAATTGCTCAGCTCACACAGCGCTGGAGAAAAGCAGAGGCGGAAAACCAACAGGATGTAAAACGTGCTCTCCTCTCAGACCATGATTGGCTTCGTGCCCTGCGGATGACTTCCTATGGGTTTCTTTTGTACGGCCCTGGTTCTTATGCCTGGTACCAGTATCTTGATCATTCTTTGCCTGCAAAAACTGTGGAGAACCTACTGCTGAAGGTTTTGTTAAATCAAATTGTGCTTGGTCCATGTGTGATTGCTGTTGTTTTTGCATGGAACAATTTATGGCAAGGGAAAGTGTCACAGCTTCCAGGGAAGTACCAAAGAGATGCTCTTCCCACTTTACTTTATGGGTTTAGGTTCTGGATACCTGTCACTTTTTTGAATTTCTGGGTGGTTCCTCTTCAAGCTCGTGTAGCTTTCATGTCTGTGGGTTCAATTTTTTGGAACTTCTGCTTGTCTTCAACTATGAGCAAGTAA

Protein Analysis

227

Amino Acids

25.47

Weight (kDa)

10.09

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mpv17_PMP22 PF04117 162 - 221 8.7e-16 Mpv17 / PMP22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 363
AccB1I GGYRCC 1 cut(s) 363
AciI CCGC 6 cut(s) 15, 21, 30, 177, 251, 313
AcsI RAATTY 1 cut(s) 586
AcuI CTGAAG 1 cut(s) 437
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 3 cut(s) 341, 365, 521
AfeI AGCGCT 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 156
AgsI TTSAA 4 cut(s) 586, 608, 639, 666
AjnI CCWGG 3 cut(s) 346, 359, 511
AluBI AGCT 4 cut(s) 223, 508, 611, 620
AluI AGCT 4 cut(s) 223, 508, 611, 620
Alw21I GWGCWC 1 cut(s) 280
Aor51HI AGCGCT 1 cut(s) 233
AoxI GGCC 2 cut(s) 31, 343
ApeKI GCWGC 1 cut(s) 73
ApoI RAATTY 1 cut(s) 586
Asp718I GGTACC 1 cut(s) 363
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 2 cut(s) 344, 446
AsuHPI GGTGA 1 cut(s) 48
AvaII GGWCC 1 cut(s) 446
BaeGI GKGCMC 1 cut(s) 310
BanI GGYRCC 1 cut(s) 363
BanII GRGCYC 1 cut(s) 151
BauI CACGAG 1 cut(s) 612
BbsI GAAGAC 2 cut(s) 88, 654
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 1 cut(s) 85
BceAI ACGGC 2 cut(s) 198, 358
BciT130I CCWGG 3 cut(s) 348, 361, 513
BciVI GTATCC 1 cut(s) 561
BclI TGATCA 1 cut(s) 376
BfoI RGCGCY 1 cut(s) 235
BfuI GTATCC 1 cut(s) 561
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 4 cut(s) 22, 31, 74, 177
BlpI GCTNAGC 1 cut(s) 219
BlsI GCNGC 4 cut(s) 23, 32, 75, 178
Bme1390I CCNGG 3 cut(s) 348, 361, 513
Bme18I GGWCC 1 cut(s) 446
BmgT120I GGNCC 2 cut(s) 344, 446
BmiI GGNNCC 3 cut(s) 161, 365, 600
BmrFI CCNGG 3 cut(s) 348, 361, 513
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 1 cut(s) 520
BmuI ACTGGG 1 cut(s) 100
BpiI GAAGAC 2 cut(s) 88, 654
BpmI CTGGAG 1 cut(s) 256
Bpu1102I GCTNAGC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 85
BsaJI CCNNGG 3 cut(s) 180, 346, 512
BsaWI WCCGGW 1 cut(s) 140
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 4 cut(s) 106, 161, 198, 367
BseBI CCWGG 3 cut(s) 348, 361, 513
BseDI CCNNGG 3 cut(s) 180, 346, 512
BseGI GGATG 2 cut(s) 271, 321
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMII CTCAG 2 cut(s) 233, 300
BseNI ACTGG 4 cut(s) 106, 161, 198, 367
BseRI GAGGAG 3 cut(s) 40, 103, 272
BseSI GKGCMC 1 cut(s) 310
BseXI GCAGC 1 cut(s) 85
BshFI GGCC 2 cut(s) 33, 345
BshNI GGYRCC 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 156
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 2 cut(s) 33, 345
Bsp1286I GDGCHC 3 cut(s) 151, 280, 310
Bsp143I GATC 1 cut(s) 376
Bsp1720I GCTNAGC 1 cut(s) 219
BspACI CCGC 6 cut(s) 15, 21, 30, 177, 251, 313
BspANI GGCC 2 cut(s) 33, 345
BspCNI CTCAG 2 cut(s) 232, 299
BspLI GGNNCC 3 cut(s) 161, 365, 600
BspQI GCTCTTC 2 cut(s) 139, 540
BspT107I GGYRCC 1 cut(s) 363
BsrI ACTGG 4 cut(s) 106, 161, 198, 367
BssECI CCNNGG 3 cut(s) 180, 346, 512
BssMI GATC 1 cut(s) 376
BssNI GRCGYC 1 cut(s) 85
BssSI CACGAG 1 cut(s) 612
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 3 cut(s) 348, 361, 513
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 3 cut(s) 139, 540, 609
BstACI GRCGYC 1 cut(s) 85
BstC8I GCNNGC 3 cut(s) 174, 187, 390
BstDEI CTNAG 2 cut(s) 219, 286
BstDSI CCRYGG 1 cut(s) 180
BstF5I GGATG 2 cut(s) 271, 321
BstH2I RGCGCY 1 cut(s) 235
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 1 cut(s) 379
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 6 cut(s) 21, 27, 30, 182, 229, 617
BstNI CCWGG 3 cut(s) 348, 361, 513
BstSCI CCNGG 3 cut(s) 346, 359, 511
BstSLI GKGCMC 1 cut(s) 310
BstV1I GCAGC 1 cut(s) 85
BstV2I GAAGAC 2 cut(s) 88, 654
BstXI CCANNNNNNTGG 1 cut(s) 130
BsuI GTATCC 1 cut(s) 561
BsuRI GGCC 2 cut(s) 33, 345
BtgI CCRYGG 1 cut(s) 180
BtsCI GGATG 2 cut(s) 271, 321
BtsIMutI CAGTG 2 cut(s) 154, 191
Cac8I GCNNGC 3 cut(s) 174, 187, 390
CfoI GCGC 1 cut(s) 234
Cfr13I GGNCC 2 cut(s) 344, 446
CseI GACGC 2 cut(s) 93, 143
Csp6I GTAC 3 cut(s) 340, 364, 520
CspCI CAANNNNNGTGG 2 cut(s) 381, 416
CviAII CATG 4 cut(s) 293, 450, 474, 625
CviQI GTAC 3 cut(s) 340, 364, 520
DdeI CTNAG 2 cut(s) 219, 286
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
Eam1104I CTCTTC 3 cut(s) 139, 540, 609
EarI CTCTTC 3 cut(s) 139, 540, 609
EciI GGCGGA 1 cut(s) 266
Eco24I GRGCYC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 446
Eco47III AGCGCT 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 437
EcoRII CCWGG 3 cut(s) 346, 359, 511
EcoT38I GRGCYC 1 cut(s) 151
FaeI CATG 4 cut(s) 296, 453, 477, 628
FatI CATG 4 cut(s) 292, 449, 473, 624
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 4 cut(s) 22, 31, 74, 177
FokI GGATG 2 cut(s) 278, 328
FriOI GRGCYC 1 cut(s) 151
Fsp4HI GCNGC 4 cut(s) 22, 31, 74, 177
GlaI GCGC 1 cut(s) 233
GluI GCNGC 4 cut(s) 22, 31, 74, 177
GsuI CTGGAG 1 cut(s) 256
HaeII RGCGCY 1 cut(s) 235
HaeIII GGCC 2 cut(s) 33, 345
HapII CCGG 1 cut(s) 141
HgaI GACGC 2 cut(s) 93, 143
HhaI GCGC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 85
Hin1II CATG 4 cut(s) 296, 453, 477, 628
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HinfI GANTC 1 cut(s) 95
HpaII CCGG 1 cut(s) 141
HphI GGTGA 1 cut(s) 48
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 2 cut(s) 201, 289
Hpy188III TCNNGA 2 cut(s) 374, 565
Hpy8I GTNNAC 1 cut(s) 133
HpyAV CCTTC 1 cut(s) 412
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 1 cut(s) 274
HpyCH4V TGCA 3 cut(s) 172, 392, 473
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 27, 30, 182, 229, 617
HpyF3I CTNAG 2 cut(s) 219, 286
HpySE526I ACGT 1 cut(s) 274
Hsp92I GRCGYC 1 cut(s) 85
Hsp92II CATG 4 cut(s) 296, 453, 477, 628
HspAI GCGC 1 cut(s) 232
KpnI GGTACC 1 cut(s) 367
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 1 cut(s) 376
LguI GCTCTTC 2 cut(s) 139, 540
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 1 cut(s) 520
MaeII ACGT 1 cut(s) 274
MaeIII GTNAC 3 cut(s) 152, 501, 574
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 5 cut(s) 93, 156, 527, 596, 654
MfeI CAATTG 1 cut(s) 213
MhlI GDGCHC 3 cut(s) 151, 280, 310
MluCI AATT 7 cut(s) 118, 124, 213, 435, 481, 586, 639
MmeI TCCRAC 1 cut(s) 23
MnlI CCTC 7 cut(s) 44, 61, 121, 124, 241, 293, 612
MseI TTAA 2 cut(s) 167, 428
MslI CAYNNNNRTG 1 cut(s) 629
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 3 cut(s) 348, 361, 513
MunI CAATTG 1 cut(s) 213
Mva1269I GAATGC 1 cut(s) 10
MvaI CCWGG 3 cut(s) 348, 361, 513
MwoI GCNNNNNNNGC 6 cut(s) 21, 27, 30, 182, 229, 617
NdeII GATC 1 cut(s) 376
NlaIII CATG 4 cut(s) 296, 453, 477, 628
NlaIV GGNNCC 3 cut(s) 161, 365, 600
NmuCI GTSAC 3 cut(s) 152, 501, 574
PciSI GCTCTTC 2 cut(s) 139, 540
PctI GAATGC 1 cut(s) 10
PfeI GAWTC 1 cut(s) 95
PkrI GCNGC 4 cut(s) 23, 32, 75, 178
Psp6I CCWGG 3 cut(s) 346, 359, 511
PspGI CCWGG 3 cut(s) 346, 359, 511
PspN4I GGNNCC 3 cut(s) 161, 365, 600
PspPI GGNCC 2 cut(s) 344, 446
RsaI GTAC 3 cut(s) 341, 365, 521
RsaNI GTAC 3 cut(s) 340, 364, 520
RseI CAYNNNNRTG 1 cut(s) 629
SapI GCTCTTC 2 cut(s) 139, 540
SaqAI TTAA 2 cut(s) 167, 428
SatI GCNGC 4 cut(s) 22, 31, 74, 177
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 2 cut(s) 344, 446
ScrFI CCNGG 3 cut(s) 348, 361, 513
SduI GDGCHC 3 cut(s) 151, 280, 310
SetI ASST 9 cut(s) 225, 277, 411, 423, 510, 563, 574, 613, 622
SfaNI GCATC 1 cut(s) 520
SinI GGWCC 1 cut(s) 446
SmiMI CAYNNNNRTG 1 cut(s) 629
Sse9I AATT 7 cut(s) 118, 124, 213, 435, 481, 586, 639
SsiI CCGC 6 cut(s) 15, 21, 30, 177, 251, 313
StyD4I CCNGG 3 cut(s) 346, 359, 511
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 1 cut(s) 277
TaqI TCGA 1 cut(s) 93
TasI AATT 7 cut(s) 118, 124, 213, 435, 481, 586, 639
TauI GCSGC 3 cut(s) 24, 33, 179
TfiI GAWTC 1 cut(s) 95
Tru1I TTAA 2 cut(s) 167, 428
Tru9I TTAA 2 cut(s) 167, 428
TscAI CASTG 2 cut(s) 161, 198
TseFI GTSAC 3 cut(s) 152, 501, 574
TseI GCWGC 1 cut(s) 73
Tsp45I GTSAC 3 cut(s) 152, 501, 574
TspDTI ATGAA 2 cut(s) 17, 613
TspRI CASTG 2 cut(s) 161, 198
VpaK11BI GGWCC 1 cut(s) 446
XapI RAATTY 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.